CN105219656B - A kind of Fusarium oxysporum and its application in Growth of Dendrobium candidum is promoted - Google Patents
A kind of Fusarium oxysporum and its application in Growth of Dendrobium candidum is promoted Download PDFInfo
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- CN105219656B CN105219656B CN201510624601.4A CN201510624601A CN105219656B CN 105219656 B CN105219656 B CN 105219656B CN 201510624601 A CN201510624601 A CN 201510624601A CN 105219656 B CN105219656 B CN 105219656B
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Abstract
The invention discloses a kind of Fusarium oxysporum, its Classification And Nomenclature is fusarium oxysporum (Fusarium oxysporum), bacterial strain number is JSNL004, it has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.11310, and preservation date is September in 2015 10.Fusarium oxysporum JSNL004 of the present invention is notable in raising Growth of Dendrobium candidum speed etc. action effect；And can manually cultivate, reproduction speed is fast, is convenient for large-scale production, has good development prospect.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of Fusarium oxysporum and its in dendrobium candidum is disease-resistant
Using.
Background technology
Dendrobium candidum is the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family Dendrobium, and research shows, is sprouted from its seed to the strong of seedling
Kang Shengchang, without the participation for not needing symbiotic effects.Because dendrobium officinale resource is on the verge of exhaustion, at present, the city of dendrobium candidum
Field demand is mainly by artificial cultivation, but artificial growth Growth of Dendrobium candidum speed is slow, and average product is low.Mycorhiza is mycorhiza in soil
The homobium with specific modality 26S Proteasome Structure and Function that fungi is formed with root system of plant, is that one kind of generally existing in nature is total to
Raw phenomenon.Orchid is stronger to the dependence of mycorhiza, mycorrhizal fungi and the conditional formation homobium of root system of plant, in physiology
On be a mutually coordinated entirety, and during common evolutionary, form specific morphological feature.Mycorrhizal fungi and host
The correlation of plant is mutually beneficial in most cases, and mycorrhizal fungi obtains nutriment necessary to growth out of plant, and
Plant also can preferably obtain required nutrient and moisture etc. by being combined with symbiotic effects from soil.Rationally utilize mycorhiza
Resource, improve the resistance against diseases of dendrobium candidum, to a certain extent reduce chemical pesticide amount of application, this also comply with agricultural can
Sustainable development strategy.Therefore, bush mycorrhiza agent correlative study has turned into an important directions of dendrobium candidum industry.
The content of the invention
It is true that the technical problems to be solved by the invention are to provide a kind of to dendrobium candidum growth-promoting functions significant effect mycorhiza
Bacterium --- Ascomycotina Fusarium fusarium oxysporum (Fusarium oxysporum) JSNL004.
The present invention also technical problems to be solved are to provide application of the above-mentioned fungi in Growth of Dendrobium candidum speed is improved.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
A kind of Fusarium oxysporum, its Classification And Nomenclature are fusarium oxysporum (Fusarium oxysporum), and bacterial strain number is
JSNL004, China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number have been preserved in it
For CGMCC No.11310, preservation date is September in 2015 10.Preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Number Institute of Microorganism, Academia Sinica, postcode 100101.
Above-mentioned bacterial strains JSNL004 is that in August, 2011 upgrowth situation in Yueqing City of Zhejiang Province dendrobium candidum plant area is preferable
Plant on separation obtain, the bacterial strain is notable to dendrobium candidum growth-promoting functions.
Fusarium oxysporum (Fusarium oxysporum) JSNL004 colony characteristicses are as follows：
Morphological observation shows：After being cultivated on PDA puts down a rebellion, colonial morphology is visually observed, it is found that its mycelium is in
Villiform, and slightly carry milk yellow pigment (Figure 1A)；Microconidia unit cell, it is avette, it is born on single raw bottle stalk, often in bottle
Stalk top is polymerized to (Figure 1B).Amplification ITS area's complete sequences are simultaneously sequenced, and PCR expands obtained 16sRNA complete sequences such as SEQ
ID No：Shown in 1.
Application of the above-mentioned Fusarium oxysporum to dendrobium candidum growth-promoting functions is also within protection scope of the present invention.
Specifically application process is：Fusarium oxysporum (Fusarium oxysporum) JSNL004 is prepared into solid fungicide,
Dendrobium candidum seedling cultivation matrix surface is placed in, the solid fungicide inoculum concentration of every clump of dendrobium candidum seedling is 5g, after microbial inoculum,
Relative water content maintains more than 60% 10 day, to ensure that mycelia survives in the substrate.
Wherein, described solid fungicide is prepared as follows obtaining：It is by culture matrix, ammonium nitrate of cotton seed hulls bacteria residue
External source nitrogen source, sucrose are external source carbon source, are prepared into solid medium, sterilizing, inoculation fusarium oxysporum (Fusarium
Oxysporum) JSNL004, cultivated 25~30 days under the conditions of 27 DEG C, 12h illumination+12h dark, pH6-8, by cultured bacterium
Agent cuts into 1cm3The block of size, the inoculation for dendrobium candidum plant bush mycorrhiza agent.
Beneficial effect：Compared with prior art, it is an advantage of the invention that：
(1) fusarium oxysporum (Fusarium oxysporum) JSNL004 is more notable to dendrobium candidum growth-promoting functions effect.
(2) fusarium oxysporum (Fusarium oxysporum) JSNL004 condition of culture is simple, easily preserves, and is easy to industry
Metaplasia is produced, and has good development prospect.
Brief description of the drawings
Fig. 1 is dendrobium candidum mycorrhizal fungi JSNL004 morphological feature.
Fig. 2 is growth-promoting capability analysis of the JSNL004 bacterium to dendrobium candidum.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Embodiment 1：
Mycorrhizal fungi fusarium oxysporum (Fusarium oxysporum) JSNL004 is prepared into solid fungicide.It is specific to prepare
Mode is：Be external source nitrogen source using cotton seed hulls bacteria residue as culture matrix, ammonium nitrate, sucrose be external source carbon source, be prepared into solid culture
Matrix loads in autoclave bags, is sterilized 20 minutes under 121 DEG C and 101.3 kPas of condition of high voltage.Treat that culture matrix cools down
Aseptically Arbuscular Mycorrhizal Fungi fungi JSNL004 afterwards, is then manually cultivated.Specific condition of culture is 26 DEG C, 12h illumination
+ 12h is dark, pH6.5, cultivates 25~30 days.Cultured microbial inoculum is processed into 1cm3The block of size, is positioned over and plants then
The dendrobium candidum seedling cultivation matrix surface of kind.Cultivation of dendrobium officinale selects bore 15cm nutritive cube, each nutritive cube cultivation 1
Clump (5 plants) dendrobium candidum.Growing way basically identical dendrobium candidum plant is selected during inoculation, and carries out the measure of height of seedling and ground diameter.
The solid fungicide inoculum concentration of every clump of seedlings of Dendrobium officinale is 5g.After microbial inoculum, Relative water content maintains more than 60%
10 days, it is ensured that mycelia survives in the substrate.6 months after inoculation, the Mycorrhizal degree detecting of dendrobium candidum root system is carried out, it is ensured that connect
Mycorrhizal fungi fusarium oxysporum (Fusarium oxysporum) JSNL004 of kind is combined with dendrobium candidum root, forms bacterium
Root.12 months after inoculation, the growth-promoting effect analysis (table 1, Fig. 2) of dendrobium candidum Mycorrhizal plant is carried out.With non-Arbuscular Mycorrhizal Fungi bacterium
Agent, the dendrobium candidum plant for being only inoculated with equivalent cotton seed hulls bacteria residue is adjoining tree (CK).
Growth-promoting effects of the JSNL004 of table 1 to dendrobium candidum plant
Claims (4)
1. a kind of Fusarium oxysporum, its Classification And Nomenclature is fusarium oxysporum (Fusarium oxysporum), and bacterial strain number is
JSNL004, China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC have been preserved in it
No.11310, preservation date are September in 2015 10.
2. application of the Fusarium oxysporum in Growth of Dendrobium candidum is promoted described in claim 1.
3. application according to claim 2, it is characterised in that：By fusarium oxysporum (Fusarium oxysporum)
JSNL004 is prepared into solid fungicide, is placed in dendrobium candidum seedling cultivation matrix surface, the solid fungicide of every clump of dendrobium candidum seedling
Inoculum concentration is 5g, and after microbial inoculum, Relative water content maintains more than 60% 10 day, with ensure mycelia in the substrate into
It is living.
4. application according to claim 3, it is characterised in that described solid fungicide is prepared as follows obtaining：With
Cotton seed hulls bacteria residue is culture matrix, ammonium nitrate is external source nitrogen source, sucrose is external source carbon source, is prepared into solid medium, sterilizing, is connect
Kind fusarium oxysporum (Fusarium oxysporum) JSNL004, cultivated under the conditions of 27 DEG C, 12h illumination+12h dark, pH6-8
25~30 days, cultured microbial inoculum is cut into 1cm3The block of size, the inoculation for dendrobium candidum plant bush mycorrhiza agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342330A (en) * | 2018-05-09 | 2018-07-31 | 中国科学院天津工业生物技术研究所 | One plant of long shoot trichoderma and its application with broad spectrum antibacterial performance |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106978359B (en) * | 2017-05-19 | 2019-09-20 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in the anti-root rot of dendrobium candidum |
| CN106967624B (en) * | 2017-05-19 | 2019-09-10 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum and its application in officinal dendrobium stem plantation |
| CN106978358B (en) * | 2017-05-19 | 2019-09-10 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum for promoting Growth of Dendrobium candidum and its application |
| CN107475134B (en) * | 2017-09-30 | 2019-09-20 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in prevention and treatment dendrobium candidum southern blight |
| CN109182144A (en) * | 2018-09-29 | 2019-01-11 | 江苏农林职业技术学院 | The fungal elicitor and the preparation method and application thereof for promoting purple dendrobium seed to sprout |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
| CN104195055A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
-
2015
- 2015-09-25 CN CN201510624601.4A patent/CN105219656B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
| CN104195055A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
Non-Patent Citations (1)
| Title |
|---|
| 铁皮石斛内生真菌的分离鉴定及其促宿主生长作用;赵昕梅 等;《河南农业科学》;20120630;第41卷(第6期);第103页2.2部分，第104页表2 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342330A (en) * | 2018-05-09 | 2018-07-31 | 中国科学院天津工业生物技术研究所 | One plant of long shoot trichoderma and its application with broad spectrum antibacterial performance |
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