CN105112309A - Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale - Google Patents
Umbelopsis nana and application thereof in promoting growth of Dendrobium officinale Download PDFInfo
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- CN105112309A CN105112309A CN201510623700.0A CN201510623700A CN105112309A CN 105112309 A CN105112309 A CN 105112309A CN 201510623700 A CN201510623700 A CN 201510623700A CN 105112309 A CN105112309 A CN 105112309A
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Abstract
The invention discloses Umbelopsis nana, classified under Umbelopsis in Mucorales in Zygomycotina, under a bacterial number JSNL001-4, already collected in china general microbiological culture collection center under CGMCC No.11308 on 11th Sep., 2015. The Umbelopsis nana JSNL001-4 is capable of promoting growth of Dendrobium officinale, has evident effect, is suitable for artificial cultivation, is quick to breed and convenient to produce on a large scale and has a promising development and application prospect.
Description
Technical field
The invention belongs to microbial technology field, it is mould and promoting the application in Growth of Dendrobium candidum to be specifically related to a kind of short and small umbrella.
Background technology
Herba Dendrobii is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, and research shows, from its seed germination to the healthy growth of plant, invariably needs the participation of symbiotic effects.Because dendrobium officinale resource is on the verge of exhaustion, at present, the market requirement of Herba Dendrobii is mainly by artificial culture, but artificial growth Growth of Dendrobium candidum speed is slow, and mean yield is low.Mycorhiza is the symbiote with specific modality structure and function that in soil, mycorrhizal fungi and root system of plant are formed, and is the ubiquitous a kind of commensalism of occurring in nature.Orchid is comparatively strong to the dependency of mycorhiza, mycorrhizal fungi and the conditional formation symbiote of root system of plant, physiology is the entirety mutually coordinated, and in the process of common evolutionary, forms specific morphological specificity.The mutual relationship of mycorrhizal fungi and host plant is in most cases mutually beneficial, and mycorrhizal fungi obtains the required nutritive substance of growth in plant materials, and plant is by being combined with symbiotic effects, also can better obtain required nutrient and moisture etc. from soil.Appropriate application mycorhiza resource, improves the resistance against diseases of Herba Dendrobii, reduces the amount of application of chemical pesticide to a certain extent, and this also meets the strategy of sustainable development of agricultural.Therefore, bush mycorrhiza agent correlative study has become an important directions of Herba Dendrobii industry.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of mycorrhizal fungi to Herba Dendrobii growth promoting function Be very effective---the short and small umbrella of the mould genus of Zygomycotina mucorales umbrella shape mould (Umbelopsisnana).
The technical problem that the present invention also will solve is to provide above-mentioned fungi and is promoting the application in Growth of Dendrobium candidum.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of short and small umbrella is mould, its Classification And Nomenclature is the short and small umbrella of the mould genus of Zygomycotina mucorales umbrella shape mould (Umbelopsisnana), bacterial strain number is JSNL001-4, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number is CGMCCNo.11308, preservation date is on September 11st, 2015, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101.
Above-mentioned bacterial strains JSNL001-4 the good plant of upgrowth situation is separated to obtain in August, 2011 in Jurong City of Jiangsu Province Herba Dendrobii plants district, and this bacterial strain is remarkable to Herba Dendrobii growth promoting function.
The colony characteristics of short and small umbrella mould (Umbelopsisnana) JSNL001-4 is as follows:
Morphological observation shows: cultivate on PDA flat board, and mycelia is fine and close, invests substrate surface; Front is the full edge in white colony edge; Reverse side white is extremely faint yellow, slightly pigment deposition (Fig. 1).Amplification ITS district complete sequence also checks order, and the 16sRNA complete sequence that pcr amplification obtains is as shown in SEQIDNo:1.
Above-mentioned short and small umbrella is mould is promoting that the application in Growth of Dendrobium candidum is also within protection scope of the present invention.
Concrete application method is: mould for short and small umbrella (Umbelopsisnana) JSNL001-4 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
Wherein, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose is for external source carbon source, be prepared into solid medium, sterilizing, inoculate short and small umbrella mould (Umbelopsisnana) JSNL001-4,26 DEG C, cultivate 25 ~ 30 days under dark, the pH6.5 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) short and small umbrella mould (Umbelopsisnana) JSNL001-4 is comparatively remarkable to Herba Dendrobii growth-promoting functions effect, has certain effect to the use reducing chemical fertilizer, is also the important means ensureing edible safety.
(2) short and small umbrella mould (Umbelopsisnana) JSNL001-4 culture condition simply, is easily preserved, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
Fig. 1 is that JSNL001-4 bacterium colony cultivates photo.
Fig. 2 is the growth-promoting capability analysis of JSNL001-4 bacterium Mycorrhizal Herba Dendrobii.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
Mould for short and small for mycorrhizal fungi umbrella (Umbelopsisnana) JSNL001-4 is prepared into solid fungicide.Concrete preparation method is: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose for external source carbon source, be prepared into solid culture matrix and load in autoclaving bag, sterilizing 20 minutes under the condition of high voltage of 121 DEG C and 101.3 kPas.Aseptically Arbuscular Mycorrhizal Fungi fungi JSNL001-4 after culture medium cooling, then carries out artificial culture.Concrete culture condition is 26 DEG C, 12h illumination+12h is dark, pH6.5, cultivates 25 ~ 30 days.Cultured microbial inoculum is processed into 1cm
3the block of size, is positioned over the Herba Dendrobii seedling cultivation matrix surface of planting then.Cultivation of dendrobium officinale selects the nutrition pot of bore 15cm, and each nutrition pot cultivates 1 clump of (5 strain) Herba Dendrobii.Select the Herba Dendrobii plant that growing way is basically identical during inoculation, and carry out the mensuration of height of seedling and leading thread.The solid fungicide inoculum size of every clump of seedlings of Dendrobium officinale is 5g.After using microbial inoculum, Relative water content maintains more than 60% 10 day, guarantees that mycelia survives in the substrate.Inoculate latter 6 months, carry out the Mycorrhizal degree detecting of Herba Dendrobii root system, guarantee that mycorrhizal fungi two type umbrella mould (Umbelopsisdimorpha) JSNL001-4 inoculated is combined with Herba Dendrobii root, define mycorhiza.Inoculate latter 12 months, carry out the growth-promoting effect analysis (table 1, Fig. 2) of Herba Dendrobii Mycorrhizal plant.With non-Arbuscular Mycorrhizal Fungi microbial inoculum, the Herba Dendrobii plant only inoculating equivalent cotton seed hulls bacterium slag is adjoining tree (CK).
Table 1JSNL001-4 is to the growth-promoting effect of Herba Dendrobii plant
Claims (4)
1. a short and small umbrella is mould, its Classification And Nomenclature is the short and small umbrella of the mould genus of Zygomycotina mucorales umbrella shape mould (Umbelopsisnana), bacterial strain number is JSNL001-4, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCCNo.11308, and preservation date is on September 11st, 2015.
2. short and small umbrella according to claim 1 is mould is promoting the application in Growth of Dendrobium candidum.
3. application according to claim 2, it is characterized in that: mould for short and small umbrella (Umbelopsisnana) JSNL001-4 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
4. application according to claim 3, it is characterized in that, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose is for external source carbon source, be prepared into solid medium, sterilizing, inoculate short and small umbrella mould (Umbelopsisnana) JSNL001-4,26 DEG C, cultivate 25 ~ 30 days under dark, the pH6.5 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109819868A (en) * | 2019-04-09 | 2019-05-31 | 中国热带农业科学院环境与植物保护研究所 | A kind of dendrobium officinale culture medium and preparation method thereof |
| CN117887590A (en) * | 2024-02-29 | 2024-04-16 | 云南省农业科学院生物技术与种质资源研究所 | South China matsutake growth promoting fungi and the application thereof |
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| CN103881928A (en) * | 2014-03-13 | 2014-06-25 | 浙江农林大学 | Application of mortierella mycorrhiza fungi to culture of tissue culture seedling of dendrobium officinale |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109819868A (en) * | 2019-04-09 | 2019-05-31 | 中国热带农业科学院环境与植物保护研究所 | A kind of dendrobium officinale culture medium and preparation method thereof |
| CN117887590A (en) * | 2024-02-29 | 2024-04-16 | 云南省农业科学院生物技术与种质资源研究所 | South China matsutake growth promoting fungi and the application thereof |
| CN117887590B (en) * | 2024-02-29 | 2024-05-17 | 云南省农业科学院生物技术与种质资源研究所 | Nanhua tricholoma matsutake growth promoting bacterium and application thereof |
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Application publication date: 20151202 |