CN104195054B - Mould and the application in dendrobium candidum growth-promoting and drought resisting of a kind of two type umbrellas - Google Patents
Mould and the application in dendrobium candidum growth-promoting and drought resisting of a kind of two type umbrellas Download PDFInfo
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- CN104195054B CN104195054B CN201410457973.8A CN201410457973A CN104195054B CN 104195054 B CN104195054 B CN 104195054B CN 201410457973 A CN201410457973 A CN 201410457973A CN 104195054 B CN104195054 B CN 104195054B
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Abstract
The invention discloses a kind of two type umbrellas mould, does is its Classification And Nomenclature the mould (Umbelopsis of the mould genus two type umbrella of Zygomycotina Mucoales umbrella shape? dimorpha), bacterial strain number is JSNL001-1, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, does is deposit number CGMCC? No.9563, preservation date is on August 15th, 2014. The mould JSNL001-1 of the present invention's two type umbrella is remarkable at aspect action effects such as raising Growth of Dendrobium candidum speed, drought-resistant abilities; And can manually cultivate, reproduction speed is fast, be convenient to carry out large-scale production, there is good development prospect.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of two type umbrellas mould and in dendrobium candidum growth-promoting, drought resistingIn application.
Background technology
Dendrobium candidum is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, is mainly distributed in southwest, south of the River each province, has and exempts fromThe effects such as epidemic disease regulates, delays senility, are the very rare rare traditional Chinese medicines of recording in China's pharmacopeia, are some Chinese patent drugs, health careThe essential raw material element of product, has the laudatory title of " China celestial grass ", " gold in medicine ". Because orchid and fungi have natural symbiosisRelation, the healthy growth from its seed germination to seedling, needs the participation of fungi invariably, and this artificial cultivation that makes dendrobium candidum isFor difficulty, and the artificial growth speed of growth is slow, and average product is low, and disease occurs comparatively serious. At present, demand is mainly by excavatingWild resource, causes wild dendrobium candidum resource to be on the verge of exhaustion, is classified as by country the medicinal plant that two classes are laid special stress on protecting. MycorhizaBeing the homobium with specific modality 26S Proteasome Structure and Function that in soil, mycorrhizal fungi and root system of plant form, is that occurring in nature is generalA kind of symbiosis existing. Orchid is stronger to the dependence of mycorhiza, the conditional formation of mycorrhizal fungi and root system of plantHomobium is an entirety of mutually coordinating, and in common process of evolving, forms specific morphological feature on physiology.The correlation of mycorrhizal fungi and host plant is in most cases mutually beneficial, and mycorrhizal fungi is essential from obtaining growth in plantNutriment, and plant is by being combined with symbiosis fungi, also can better from soil, obtain required nutrient and moisture etc.In recent years, have many scholars the diversity of the mycorhiza structure of Dendrobium Sw, mycorrhizal fungi, mycorrhizal fungi to Dendrobium SwThe aspect such as effect research carried out large quantity research, carry out the high-efficiency artificial of dendrobium candidum for making full use of the good characteristic of mycorhizaCultivation is laid a good foundation.
Mycorrhizal fungi has certain effect to growing of plant. As the tissue culturing seedling of dendrobium candidum is carried out manuallyAfter inoculation Epulorhiza (Epulorhiza) bacterial strain 60d, connect vaccine mean fresh growth rate and gone out 84.8% than contrast height of seedling; IronAfter skin dendrobe tissue culture seedling inoculation Orchidaceous rhizoctonias Cymbidiumfaberikanran and Cymbidiumsinense, averageFresh weight growth rate has all reached the level of utmost point significant difference, inoculation Cymbidiumgoeringii and CymbidiumThe group training seedling of goeringii has reached the level of significant difference; The endogenetic fungus of Mycena can promote dendrobium candidum seedlingGrow; From the isolated GN09 of pleione bulbocodioides (Pleionebulbocodioides), GN10 and GN43 bacterial strain and ring grassAfter the symbiosis of the stem of noble dendrobium (DendrobiumloddigesiiRolfe) group training seedling is cultivated, the fresh weight growth rate of group training seedling is compared respectivelyAccording to having improved 69.8%, 61.9% and 81.7%, there is significantly great using value.
From preserving the ecological environment and the angle of food security, dendrobium candidum, as rare Chinese medicine, reduces agriculture as far as possibleThe amount of application of medicine, chemical fertilizer. Can greatly improve product quality, reach the object of safe edible, this also meet agricultural canSustainable development strategy.
Summary of the invention
Technical problem to be solved by this invention is to provide one to dendrobium candidum growth-promoting, degeneration-resistant action effect significantlyMycorrhizal fungi---the mould genus two type umbrellas of Zygomycotina Mucoales umbrella shape mould (Umbelopsisdimorpha).
The technical problem that the present invention also will solve is to provide above-mentioned fungi and is improving Growth of Dendrobium candidum speed, drought-resistant abilityIn application.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of two type umbrellas are mould, and its Classification And Nomenclature is the mould (Umbelopsis of the mould genus two type umbrella of Zygomycotina Mucoales umbrella shapeDimorpha), bacterial strain number is JSNL001-1, has now been preserved in China Committee for Culture Collection of Microorganisms's common micro-organismsCenter (CGMCC), deposit number is CGMCCNo.9563, preservation date is on August 15th, 2014. Preservation address is BeijingNo. 1, North Star West Road, Chaoyang District No. 3 Institute of Microorganism, Academia Sinica of institute, postcode 100101.
Above-mentioned bacterial strains JSNL001-1 is that in October, 2010, upgrowth situation was in Yueqing City of Zhejiang Province dendrobium candidum is planted districtOn good plant, separation obtains, and this bacterial strain has significant growth-promoting functions to dendrobium candidum, also can obviously improve dendrobium candidumDrought-resistant ability.
The colony characteristics of two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1 is as follows:
Morphological observation shows: on PDA puts down a rebellion, cultivate, white mycelium matter is close, crawls or is close to base thing surface;Bacterium colony is flat, and tow sides are white, the full edge of colony edge. But under culture conditions, be difficult for producing spore. Amplification ITS district is completeSequence also checks order, and the 16sRNA complete sequence that pcr amplification obtains is as shown in SEQIDNo:1.
The mould application in promotion Growth of Dendrobium candidum of above-mentioned two type umbrellas is also within protection scope of the present invention.
Application in the mould drought-resistant ability improving dendrobium candidum of above-mentioned two type umbrellas is also within protection scope of the present invention.
Concrete application process is: mould two type umbrellas (Umbelopsisdimorpha) JSNL001-1 is prepared into solid bacteriumAgent, is placed in dendrobium candidum seedling cultivation matrix surface, and the solid fungicide inoculum concentration of every clump of dendrobium candidum seedling is 5g, uses microbial inoculumAfter, Relative water content maintains more than 60% 10 days, to guarantee that mycelia survives in the substrate.
Wherein, described solid fungicide prepares as follows: taking cotton seed hulls bacterium slag as culture matrix, ammonium nitrate asExternal source nitrogenous source, sucrose are external source carbon source, are prepared into solid medium, and sterilizing is inoculated two type umbrellas mould under aseptic condition(Umbelopsisdimorpha) JSNL001-1 cultivates 25～35 under 25 DEG C, 12h illumination+12h dark, pH6.5 conditionMy god, cultured microbial inoculum is cut into 1cm3The block of size, for the inoculation of dendrobium candidum plant bush mycorrhiza agent.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) the two type umbrella growth-promoting of mould (Umbelopsisdimorpha) JSNL001-1 to dendrobium candidum and degeneration-resistant effectEffect is all comparatively remarkable, and the use that reduces chemical fertilizer and agricultural chemicals is had to certain effect, is also the important hand that ensures edible safetySection.
(2) two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1 condition of culture simply, easily preserve, be easy to workIndustryization is produced, and has good development prospect.
Brief description of the drawings
Fig. 1 is that JSNL001-1 bacterium colony is cultivated photo.
Fig. 2 is the growth-promoting effect of JSNL001-1 to dendrobium candidum plant.
Detailed description of the invention
According to following embodiment, the present invention may be better understood. But, those skilled in the art will readily understand, realExecute routine described content only for the present invention is described, and should also can not limit in claims described in detailInvention.
Embodiment 1:
Growth-promoting and the drought resisting of two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1 to dendrobium candidum can act on effectFruit is analyzed.
Mycorrhizal fungi two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1 is prepared into solid fungicide. Concrete systemStandby mode is: taking cotton seed hulls bacterium slag as culture matrix, ammonium nitrate is as external source nitrogenous source, sucrose are external source carbon source, is prepared into solid trainingFoster matrix packs in autoclaving bag, sterilizing 20 minutes under the condition of high voltage of 121 DEG C and 101.3 kPas. Treat that culture matrix is coldBut after under aseptic condition Arbuscular Mycorrhizal Fungi fungi JSNL001-1, then manually cultivate. Concrete condition of culture is 25 DEG C, 12hIllumination+12h dark, pH6.5, cultivate 25～35 days. Cultured microbial inoculum is processed into 1cm3The block of size, is positioned over and works asThe dendrobium candidum seedling cultivation matrix surface of year plantation. Cultivation dendrobium candidum is selected the nutritive cube of bore 15cm, each nutritive cubeCultivate 1 clump of (5 strain) dendrobium candidum. When inoculation, select the basically identical dendrobium candidum plant of growing way, and carry out height of seedling and leading threadMeasure. The solid fungicide inoculum concentration of every clump of seedlings of Dendrobium officinale is 5g. Use after microbial inoculum, Relative water content maintains 60%Above 10 days, guarantee that mycelia survives in the substrate. Inoculate latter 6 months, carry out the Mycorrhizal degree detecting of dendrobium candidum root system, reallyMycorrhizal fungi two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1 that protects inoculation is combined with dendrobium candidum root, shapeBecome mycorhiza. Inoculate latter 12 months, carry out growth-promoting and the drought resisting effect analysis (table 1, table 2) of dendrobium candidum Mycorrhizal plant. WithNot Arbuscular Mycorrhizal Fungi microbial inoculum, the dendrobium candidum plant that only inoculates equivalent cotton seed hulls bacterium slag is adjoining tree (CK).
The drought stress processing of dendrobium candidum plant: choose the Mycorrhizal dendrobium candidum of different disposal, unified watering,The soil moisture content of every basin plant is reached capacity, then coerce in warm lab simulation natural drought, moisture is constantly consumed,Coerce the mensuration of 10d, 20d, 30d degree of wilting, wilted percent. Temperature indoor temperature is 30-35 DEG C, and relative air humidity is50-60%. Process 50 strains, do 3 repetitions for every kind.
Under drought stress condition, according to following standard, the degree of suffering from drought of each processing dendrobium candidum is divided into 5 grades: 0: strongHealth; I: wilting appears in minority blade; II: wilting appears in half blade; III: blades more than half are wilted; IV: whole strain is wilted. SystemCount wilted percent and the wilting degree of each processing plant.
Wilted percent (%)=(occurring wilting symptom strain number/processing strain number) × 100%
Wilting degree=[∑ (wilting progression × strain number) × 100]/(the highest number of wilting × total strain number)
The growth-promoting effect of table 1JSNL001-1 to dendrobium candidum plant
The action effect of table 2JSNL001-1 to dendrobium candidum plant drought-resistant ability
Claims (7)
1. a type umbrella is mould, and its Classification And Nomenclature is the mould (Umbelopsis of the mould genus two type umbrella of Zygomycotina Mucoales umbrella shapeDimorpha), bacterial strain number is JSNL001-1, has now been preserved in China Committee for Culture Collection of Microorganisms's common micro-organismsCenter, deposit number is CGMCCNo.9563, preservation date is on August 15th, 2014.
2. the mould application promoting in Growth of Dendrobium candidum of two type umbrellas claimed in claim 1.
3. application according to claim 2, is characterized in that, by mould two type umbrellas (Umbelopsisdimorpha)JSNL001-1 is prepared into solid fungicide, is placed in dendrobium candidum seedling cultivation matrix surface, the solid bacterium of every clump of dendrobium candidum seedlingAgent inoculum concentration is 5g, uses after microbial inoculum, and Relative water content maintains more than 60% 10 days, to guarantee that mycelia becomes in the substrateLive.
4. application according to claim 3, is characterized in that, described solid fungicide prepares as follows: withCotton seed hulls bacterium slag is that culture matrix, ammonium nitrate are that external source nitrogenous source, sucrose are external source carbon source, is prepared into solid medium, sterilizing, nothingUnder bacterium condition, inoculate two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1,25 DEG C, 12h illumination+12h dark,Under pH6.5 condition, cultivate 25～35 days, cultured microbial inoculum is cut into 1cm3The block of size, for dendrobium candidum plantThe inoculation of bush mycorrhiza agent.
5. the application in the mould drought-resistant ability improving dendrobium candidum of two type umbrellas claimed in claim 1.
6. application according to claim 5, is characterized in that, by mould two type umbrellas (Umbelopsisdimorpha)JSNL001-1 is prepared into solid fungicide, is placed in dendrobium candidum seedling cultivation matrix surface, the solid bacterium of every clump of dendrobium candidum seedlingAgent inoculum concentration is 5g, uses after microbial inoculum, and Relative water content maintains more than 60% 10 days, to guarantee that mycelia becomes in the substrateLive.
7. application according to claim 6, is characterized in that, described solid fungicide prepares as follows: withCotton seed hulls bacterium slag is that culture matrix, ammonium nitrate are that external source nitrogenous source, sucrose are external source carbon source, is prepared into solid medium, sterilizing, nothingUnder bacterium condition, inoculate two type umbrellas mould (Umbelopsisdimorpha) JSNL001-1,25 DEG C, 12h illumination+12h dark,Under pH6.5 condition, cultivate 25～35 days, cultured microbial inoculum is cut into 1cm3The block of size, for dendrobium candidum plantThe inoculation of bush mycorrhiza agent.
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| CN105039173B (en) * | 2015-07-08 | 2017-11-03 | 西北大学 | Raw mortierella sp and its application in a kind of serrate clubmoss herb |
| CN105112308B (en) * | 2015-09-25 | 2018-03-09 | 江苏农林职业技术学院 | An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting |
| CN105132293B (en) * | 2015-09-25 | 2018-03-09 | 江苏农林职业技术学院 | A kind of Alternaria tenuissima and its application in dendrobium candidum growth-promoting, drought resisting |
| CN105219656B (en) * | 2015-09-25 | 2018-03-30 | 江苏农林职业技术学院 | A kind of Fusarium oxysporum and its application in Growth of Dendrobium candidum is promoted |
| CN117887590B (en) * | 2024-02-29 | 2024-05-17 | 云南省农业科学院生物技术与种质资源研究所 | Nanhua tricholoma matsutake growth promoting bacterium and application thereof |
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| WO2010015024A1 (en) * | 2008-08-05 | 2010-02-11 | Hexima Limited | Plant anti-pathogen systems |
| CN103555609A (en) * | 2013-09-23 | 2014-02-05 | 云南瑞升埃比特生物科技有限公司 | Biocontrol bacillus with effects of resisting diseases and promoting growth and application |
| WO2014078900A1 (en) * | 2012-11-23 | 2014-05-30 | Hexima Limited | Anti-pathogenic methods |
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| CN101307298A (en) * | 2008-01-30 | 2008-11-19 | 珠海市农业科学研究中心 | Broad-spectrum antifungal bacillus licheniformis and uses thereof |
| WO2010015024A1 (en) * | 2008-08-05 | 2010-02-11 | Hexima Limited | Plant anti-pathogen systems |
| WO2014078900A1 (en) * | 2012-11-23 | 2014-05-30 | Hexima Limited | Anti-pathogenic methods |
| CN103555609A (en) * | 2013-09-23 | 2014-02-05 | 云南瑞升埃比特生物科技有限公司 | Biocontrol bacillus with effects of resisting diseases and promoting growth and application |
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