CN105112308B - An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting - Google Patents
An a kind of beading are red mould and its in dendrobium candidum growth-promoting and the application in drought resisting Download PDFInfo
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- CN105112308B CN105112308B CN201510623045.9A CN201510623045A CN105112308B CN 105112308 B CN105112308 B CN 105112308B CN 201510623045 A CN201510623045 A CN 201510623045A CN 105112308 B CN105112308 B CN 105112308B
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- beading
- dendrobium candidum
- red mould
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Abstract
The invention discloses a kind of beading gibberella, its Classification And Nomenclature is that an Ascomycotina Gibberella beading are red mould (Gibberella moniliformis), bacterial strain number is JSNL003, it has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCC No.11309, and preservation date is September in 2015 11.The red mould JSNL003 of a beading of the present invention is notable in raising Growth of Dendrobium candidum speed, drought-resistant ability etc. action effect；And can manually cultivate, reproduction speed is fast, is convenient for large-scale production, has good development prospect.
Description
Technical field
The invention belongs to microbial technology field, and in particular to an a kind of beading are red mould and its in dendrobium candidum growth-promoting, drought resisting
In application.
Background technology
Dendrobium candidum is the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family Dendrobium, has immunological regulation, anti-aging and other effects, is
Extremely rare rare traditional Chinese medicine described in China's pharmacopeia, it is the required raw material element of some Chinese patent drugs, health products.Mycorhiza is soil
The homobium with specific modality 26S Proteasome Structure and Function that middle mycorrhizal fungi is formed with root system of plant, is generally existing in nature
A kind of symbiosis.Orchid is stronger to the dependence of mycorhiza, mycorrhizal fungi and the conditional formation homobium of root system of plant,
It is being physiologically a mutually coordinated entirety, and during common evolutionary, is forming specific morphological feature.Due to orchid family
Plant and fungi have natural symbiosis, sprout the healthy growth to seedling from its seed, and nothing does not need the participation of fungi, this
So that the artificial cultivation of dendrobium candidum is more difficult, and the artificial growth speed of growth is slow, and average product is low, and disease occurs more
Seriously.At present, demand causes dendrobium officinale resource to be on the verge of exhaustion, is classified as two by country mainly by excavation wild resource
The medicinal plant that class is laid special stress on protecting.The correlation of mycorrhizal fungi and host plant is mutually beneficial, mycorrhizal fungi in most cases
Nutriment necessary to growth is obtained out of plant, and plant is with symbiotic effects by being combined, also can be preferably from soil
Obtain required nutrient and moisture etc..In recent years, have many scholars the mycorhiza structure of Dendrobium Sw, mycorrhizal fungi it is various
Property, mycorrhizal fungi have carried out numerous studies to Effect study of Dendrobium Sw etc., to make full use of the excellent spy of mycorhiza
Property carry out dendrobium candidum high-efficiency artificial cultivation lay a good foundation.
Mycorrhizal fungi has certain effect to growing for plant.Such as the tissue culturing seedling of dendrobium candidum is carried out manually
After being inoculated with Epulorhiza (Epulorhiza) bacterial strain 60d, connect vaccine mean fresh growth rate and gone out 84.8% than control height of seedling；Iron
It is average after skin dendrobe tissue culture seedling inoculation Orchidaceous rhizoctonias Cymbidium faberi kanran and Cymbidium sinense
Fresh weight growth rate has reached the level of pole significant difference, inoculation Cymbidium goeringii and Cymbidium
Goeringii tissue-cultured seedling has reached the level of significant difference；The endogenetic fungus of Mycena can promote dendrobium candidum seedling
Grow；GN 09, GN 10 and the bacterial strains of GN 43 and the ring grass isolated from pleione bulbocodioides (Pleione bulbocodioides)
After the stem of noble dendrobium (Dendrobium loddigesii Rolfe) tissue-cultured seedling symbiosis culture, the fresh weight growth rate of tissue-cultured seedling compares respectively
According to improving 69.8%, 61.9% and 81.7%, significantly with great application value.Pacify from preserving the ecological environment with food
Full angle is set out, and amount of application of the dendrobium candidum as rare Chinese medicine, as far as possible chemical fertilizer, can greatly improve product product
Matter, reach the purpose of safe edible, this also complies with the strategy of sustainable development of agricultural.
The content of the invention
The technical problems to be solved by the invention be to provide it is a kind of to dendrobium candidum growth-promoting, degeneration-resistant action effect significantly
Mycorrhizal fungi --- an Ascomycotina Gibberella beading are red mould (Gibberella moniliformis).
The present invention also technical problems to be solved are to provide above-mentioned fungi and are improving Growth of Dendrobium candidum speed, drought-resistant ability
In application.
In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
An a kind of beading are red mould, and its Classification And Nomenclature is the red mould (Gibberella of an Ascomycotina Gibberella beading
Moniliformis), bacterial strain number is JSNL003, has been preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life
Thing center (CGMCC), deposit number are CGMCC No.11309, and preservation date is September in 2015 11.Preservation address is Beijing
No. 3 Institute of Microorganism, Academia Sinica of institute of city Chaoyang District North Star West Road 1, postcode 100101.
Above-mentioned bacterial strains JSNL003 is that in August, 2012 upgrowth situation in Jurong City of Jiangsu Province dendrobium candidum plant area is preferable
Plant on separation obtain, the bacterial strain has significant growth-promoting functions to dendrobium candidum, can also be obviously improved dendrobium candidum
Drought-resistant ability.
Red mould (Gibberella moniliformis) JSNL003 of beading colony characteristicses are as follows：
Morphological observation shows：Cultivated on PDA plate, mycelia canescence, loose, bacterium colony is uneven, positive and negative
Two sides is canescence, colony edge full edge.Amplification ITS area's complete sequences are simultaneously sequenced, and PCR expands obtained 16sRNA total orders
Row such as SEQ ID No：Shown in 1.
The above-mentioned red mould application in Growth of Dendrobium candidum is promoted of a beading is also within protection scope of the present invention.
The above-mentioned red mould application in the drought-resistant ability for improving dendrobium candidum of a beading is also within protection scope of the present invention.
Specifically application process is：The red mould JSNL003 of a beading is prepared into solid fungicide, is placed in the cultivation of dendrobium candidum seedling
Stromal surface, the solid fungicide inoculum concentration of every clump of dendrobium candidum seedling is 5g, and after microbial inoculum, Relative water content maintains
More than 60% 10 day, to ensure that mycelia survives in the substrate.
Wherein, described solid fungicide is prepared as follows obtaining：
Be external source nitrogen source using cotton seed hulls bacteria residue as culture matrix, ammonium sulfate, glucose be external source carbon source, be prepared into solid training
Base is supported, is sterilized, red mould (Gibberella moniliformis) JSNL003 of a beading is inoculated with aseptic condition, in 27 DEG C, 12h light
According to being cultivated 20~25 days under the conditions of+12h dark, pH6.5, cultured microbial inoculum is cut into 1cm3The block of size, is used for
The inoculation of dendrobium candidum plant bush mycorrhiza agent.
Beneficial effect：Compared with prior art, it is an advantage of the invention that：
(1) growth-promotings and degeneration-resistant work of red mould (Gibberella moniliformis) JSNL003 of a beading to dendrobium candidum
With effect more significantly, there is certain effect to the use for reducing chemical fertilizer and agricultural chemicals, and ensure the important of edible safety
Means.
(2) red mould (Gibberella moniliformis) the JSNL003 condition of culture of a beading is simple, easily preserves, and is easy to
Industrialized production, there is good development prospect.
Brief description of the drawings
Fig. 1 is JSNL003 bacterium colony culture photos.
Fig. 2 is growth-promoting effects of the JSNL003 to dendrobium candidum plant.
Embodiment
According to following embodiments, the present invention may be better understood.It is however, as it will be easily appreciated by one skilled in the art that real
Apply the content described by example and be merely to illustrate the present invention, without should be also without limitation on sheet described in detail in claims
Invention.
Embodiment 1：
Red mould (Gibberella moniliformis) JSNL003 of a beading makees to the growth-promoting and drought-resistant ability of dendrobium candidum
Use effect analysis.
Red mould (Gibberella moniliformis) JSNL003 of a mycorrhizal fungi beading is prepared into solid fungicide.Specifically
Preparation method is：Be external source nitrogen source using cotton seed hulls bacteria residue as culture matrix, ammonium sulfate, glucose be external source carbon source, be prepared into solid
Body culture matrix loads in autoclave bags, is sterilized 20 minutes under 121 DEG C and 101.3 kPas of condition of high voltage.Treat culture medium
Aseptically Arbuscular Mycorrhizal Fungi fungi JSNL003 after matter cooling, is then manually cultivated.Specific condition of culture is 27 DEG C,
12h illumination+12h is dark, pH6.5, cultivates 20~25 days.Cultured microbial inoculum is processed into 1cm3The block of size, place
In the dendrobium candidum seedling cultivation matrix surface planted then.Cultivation of dendrobium officinale selects bore 15cm nutritive cube, Mei Geying
Support pot culture and train 1 clump of (5 plants) dendrobium candidum.Growing way basically identical dendrobium candidum plant is selected during inoculation, and carries out height of seedling and ground
The measure in footpath.The solid fungicide inoculum concentration of every clump of seedlings of Dendrobium officinale is 5g.After microbial inoculum, Relative water content maintains
More than 60% 10 day, it is ensured that mycelia survives in the substrate.6 months after inoculation, the Mycorrhizal degree inspection of dendrobium candidum root system is carried out
Survey, it is ensured that red mould (Gibberella moniliformis) JSNL003 of a mycorrhizal fungi beading of inoculation with dendrobium candidum root
With reference to foring mycorhiza.12 months after inoculation, carry out dendrobium candidum Mycorrhizal plant growth-promoting and drought resisting effect analysis (table 1,
Table 2).With non-Arbuscular Mycorrhizal Fungi microbial inoculum, the dendrobium candidum plant for being only inoculated with equivalent cotton seed hulls bacteria residue is adjoining tree (CK).
The drought stress processing of dendrobium candidum plant：The Mycorrhizal dendrobium candidum of different disposal is chosen, is uniformly watered,
The soil moisture content of every basin plant is reached saturation, then coerced in warm lab simulation natural drought, moisture is constantly consumed,
Coerce the measure that 10d, 20d, 30d carry out wilting degree, wilted percent.Warm indoor temperature is 30-35 DEG C, and relative air humidity is
50-60%.Every kind of 60 plants of processing, does 3 repetitions.
Under drought stress conditions, the degree of suffering from drought of each processing dendrobium candidum is divided into 5 grades according to following standard：0：It is strong
Health；Ⅰ：A small number of blades are wilted；Ⅱ：Half blade is wilted；Ⅲ：Blades more than half are wilted；Ⅳ：Whole strain is wilted.System
Count the wilted percent and wilting degree of each processing plant.
Wilted percent (%)=(wilting symptom strain number/processing strain number occur) × 100%
Wilting degree=[∑ (wilting series × strain number) × 100]/(wilting highest series × total strain number)
Growth-promoting effects of the JSNL003 of table 1 to dendrobium candidum plant
Action effects of the JSNL003 of table 2 to dendrobium candidum plant drought-resistant ability
Claims (7)
1. an a kind of beading are red mould, its Classification And Nomenclature is the red mould (Gibberella of an Ascomycotina Gibberella beading
Moniliformis), bacterial strain number is JSNL003, has been preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life
Thing center, deposit number are CGMCC No.11309, and preservation date is September in 2015 11.
2. the red mould application in Growth of Dendrobium candidum is promoted of a beading described in claim 1.
3. application according to claim 2 a, it is characterised in that beading are red mould (Gibberella moniliformis)
JSNL003 is prepared into solid fungicide, is placed in dendrobium candidum seedling cultivation matrix surface, the solid fungicide of every clump of dendrobium candidum seedling
Inoculum concentration is 5g, and after microbial inoculum, Relative water content maintains more than 60% 10 day, with ensure mycelia in the substrate into
It is living.
4. application according to claim 3, it is characterised in that described solid fungicide is prepared as follows obtaining：With
Cotton seed hulls bacteria residue is culture matrix, ammonium sulfate is external source nitrogen source, glucose is external source carbon source, is prepared into solid medium, sterilizing,
Under aseptic condition be inoculated with red mould (Gibberella moniliformis) JSNL003 of a beading, 27 DEG C, 12h illumination+12h it is black
Secretly, cultivated 20~25 days under the conditions of pH6.5, cultured microbial inoculum is cut into 1cm3The block of size, for dendrobium candidum
The inoculation of plant bush mycorrhiza agent.
5. the red mould application in the drought-resistant ability for improving dendrobium candidum of a beading described in claim 1.
6. application according to claim 5 a, it is characterised in that beading are red mould (Gibberella moniliformis)
JSNL003 is prepared into solid fungicide, is placed in dendrobium candidum seedling cultivation matrix surface, the solid fungicide of every clump of dendrobium candidum seedling
Inoculum concentration is 5g, and after microbial inoculum, Relative water content maintains more than 60% 10 day, with ensure mycelia in the substrate into
It is living.
7. application according to claim 6, it is characterised in that described solid fungicide is prepared as follows obtaining：With
Cotton seed hulls bacteria residue is culture matrix, ammonium sulfate is external source nitrogen source, glucose is external source carbon source, is prepared into solid medium, sterilizing,
Under aseptic condition be inoculated with red mould (Gibberella moniliformis) JSNL003 of a beading, 27 DEG C, 12h illumination+12h it is black
Secretly, cultivated 20~25 days under the conditions of pH6.5, cultured microbial inoculum is cut into 1cm3The block of size, for dendrobium candidum
The inoculation of plant bush mycorrhiza agent.
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| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
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| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
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