CN101953261A - Application of jewel orchid endophytic fungi to jewel orchid seedlings - Google Patents
Application of jewel orchid endophytic fungi to jewel orchid seedlings Download PDFInfo
- Publication number
- CN101953261A CN101953261A CN2009102100732A CN200910210073A CN101953261A CN 101953261 A CN101953261 A CN 101953261A CN 2009102100732 A CN2009102100732 A CN 2009102100732A CN 200910210073 A CN200910210073 A CN 200910210073A CN 101953261 A CN101953261 A CN 101953261A
- Authority
- CN
- China
- Prior art keywords
- rhizoctonia
- terminal bud
- roxburgh anoectochilus
- anoectochilus terminal
- gxsh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to application of endophytic fungi, in particular to the application of symbiotic cultivation of jewel orchid endophytic fungi and jewel orchid tissue culturing seedlings. The endophytic fungi are Rhizoctonia spp. and are separated from the root part of the wild jewel orchid by a tissue separation method. The Rhizoctonia spp. is collected in China General Microbiological Culture Collection Center (CGMCC for short), and the address is Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing. The Rhizoctonia spp. infects the jewel orchid tissue culturing seedlings to form symbiotic seedlings, so that the application can improve stress resistance of the jewel orchid tissue culturing seedlings, improve survival rate and shorten growth cycle. Therefore, the production cost is reduced and industrialized production is realized.
Description
Technical field:
The present invention relates to a kind of application of endogenetic fungus, specially refer to the application of a kind of roxburgh anoectochilus terminal bud endogenetic fungus in roxburgh anoectochilus terminal bud group cultivation seedling.
Background technology:
Roxburgh anoectochilus terminal bud belongs to the herbaceos perennial that the orchid family is opened the lip Cymbidium, is the wider a kind of traditional valuable ingredient of traditional Chinese medicine of use among the people, is used for the treatment of pulmonary tuberculosis, cough with lung heat, rheumatic arthritis etc. more.In recent years, roxburgh anoectochilus terminal bud was used for the treatment of the obtained effects of difficult and complicated illness such as hypertension, diabetes and tumour, caused the attention of the world of medicine day by day, and especially in Taiwan, roxburgh anoectochilus terminal bud has the title of " king of Chinese medicine ".
Yet roxburgh anoectochilus terminal bud is very rare in the natural world resource, and wherein reason is very harsh and be subjected to natural world small animal and people's the destruction to the requirement of ecotope except it, and main is that the seed of roxburgh anoectochilus terminal bud is very little, does not have complete embryo for its growth.Under field conditions (factors), need infecting for it provides nutrition of endogenetic fungus just can develop into seedling, this also is universal characteristics of orchid.Endogenetic fungus provides the nutriment except can be orchid, can also promote its absorption to mineral matter, produce required vitamin of growth and development of plants and plant hormone substance, strengthen stress resistance of plant and premunition (being published in " Shanghai Agricultural journal " the 2005.21st (3) phase " function of orchid endogenetic fungus and application prospect [J] ") with reference to Zhou Deping etc.This shows, adopt the method for roxburgh anoectochilus terminal bud tissue cultivating seedling and mycorrhizal fungi symbiosis, advantages such as can promoting plant growing, enhancing stress resistance of plant and premunition are arranged, can overcome present tissue cultivating seedling poor growth, weak, the more high shortcomings of lethality of resistance.At present, aspect anoectochilus symbiosis, abroad do not report as yet, domestic Guo adopts the little mushroom of the stem of noble dendrobium (Mycenadendrobii) along magnitude, Mycen osmundicola (Mycena osmundicola), Mycena orchidicola (Mycenaorchidicola), Mycena anoectochila) (Mycena anoectochila) and anoectochilus symbiosis (number of patent application: 02100779.9; The patent No.: ZL 200510117703.3), effect unsatisfactory (high slightly, Guo is along magnitude " middle medicinal herbs " the 2002.33rd (6) interim " three kinds of endogenetic fungus are to the research of dendrobium candidum, roxburgh anoectochilus terminal bud growth effect ").
Summary of the invention:
The object of the present invention is to provide a kind ofly can form symbiotic relation and promote it to grow, improve its reproduction rate, survival rate, and strengthen the application of the roxburgh anoectochilus terminal bud endogenetic fungus of its resistance with roxburgh anoectochilus terminal bud.
The application of a kind of roxburgh anoectochilus terminal bud endogenetic fungus in roxburgh anoectochilus terminal bud is grown seedlings, this endogenetic fungus are rhizoctonia (Rhizoctonia spp.).
Described rhizoctonia (Rhizoctonia spp.) is rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6; These three bacterial strains all have been preserved in China Microbial Culture Preservation Commission common micro-organisms center, the address is a Datun Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, its deposit number and date are respectively JSH-1:CGMCCNO.3074, preservation date on May 20th, 2009; GXSH-5:CGMCC NO.3273, preservation date on September 7th, 2009; GXSH-6:CGMCC NO.3274, preservation date on September 7th, 2009.
Described rhizoctonia (Rhizoctonia sp.) JSH-1 adopts tissue isolation from the separating obtained mycorrhizal fungi of wild roxburgh anoectochilus terminal bud root.
Wherein, described rhizoctonia (Rhizoctonia sp.) JSH-1 solid culture is characterized as (Fig. 1, Fig. 2): 28 ℃ of cultivations on potato dextrose agar (PDA) medium, mycelia initial stage milk yellow, the later stage fawn is the homogeneous radiation shape to outgrowth, and growth rate is 2.5～3.0cm/d, aerial hyphae is more, form the donut that does not wait at interval, bacterium colony begins to occur oily yellowish-brown sclerotium after 6 days, is distributed in the donut place more.
Described rhizoctonia (Rhizoctonia sp.) JSH-1 morphological feature is (Fig. 3):
1). do not form any spore in asexual generation, the mycelia branch is hung at its origin place and is contracted, and barrier film is arranged, no clamp connection, rectangular or the acute angle of mycelia branch has sclerotium, and hyphal diameter is 4～7 μ m, most cells have two cell nucleus, and individual cells nuclear is arranged individually, and cell nucleus is rounded.
2). sexual generation, do not find.
Other two strain bacterial strain rhizoctonia (Rhizoctonia sp.) GXSH-5 of the present invention and rhizoctonia (Rhizoctonia sp.) GXSH-6 and rhizoctonia (Rhizoctonia sp.) JSH-1 have following common trait: asexual generation, do not form any spore, the mycelia branch is hung at its origin place and is contracted, barrier film is arranged, no clamp connection, the rectangular or acute angle of mycelia branch, diameter is 4～7 μ m, most cells have 3 or above cell nucleus, and sclerotium is arranged.
Anoectochilus symbiosis seedling is cultivated in described rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6 and the symbiosis of roxburgh anoectochilus terminal bud tissue cultivating seedling.
The mode that described symbiosis is cultivated is with the whole strain of roxburgh anoectochilus terminal bud group training seedling and described rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6 co-incubation.
Above-mentioned co-incubation is to carry out in symbiotic culture medium, under the aseptic condition; Perhaps also can be in cultivation medium, carry out under the open condition.
When rhizoctonia of the present invention (Rhizoctonia spp.) is used in roxburgh anoectochilus terminal bud group cultivation seedling, cultivate rhizoctonia (Rhizoctonia spp.) earlier, cultivate with the mode and the symbiosis of roxburgh anoectochilus terminal bud group training seedling of mycelia agar block or microbial inoculum then, concrete operations are as follows:
1). the making of mycelia agar block and microbial inoculum
Mycelia agar block: adopt the PDA medium, rhizoctonia (Rhizoctoniaspp.) after the activation is inoculated on the culture medium flat plate, 28 ℃ of dark culturing 2～4 days, (diameter is 0.5～1cm) or with the mycelia agar block of the transfer needle of calcination block hitting or the picking length of side 0.4～0.7cm on colony edge with sterilized card punch.
Microbial inoculum: rice bran or wheat bran and water are prepared burden according to (1: 1)～(2: 1) weight ratio, regulate water content to 55～60% (possess globule appearance but do not flow down) with PDB with hand-tight, put into the white disk that is used for sterilizing, four layers of gauze of surface coverage, 121 ℃ of sterilization 1h, rhizoctonia (Rhizoctonia spp.) the mycelia agar block of length and width 0.3～0.8cm is inserted in the cooling back, cultivates 6～10 days for dark 28 ℃, rubs with the hands broken before using.
2). rhizoctonia (Rhizoctonia spp.) and anoectochilus symbiosis type of rearing
Mode a: receive whole strain of aseptic roxburgh anoectochilus terminal bud group training seedling and rhizoctonia (Rhizoctonia spp.) mycelia agar block on the symbiotic culture medium simultaneously, each bottle inserts three strain groups training seedling, under the pure culture condition, cultivated 2～3 months for 20～25 ℃, intensity of illumination is 800～3000lx, 12 hours every days.
Mode b: be 1～5% microbial inoculum and matrix mixing with weight ratio, place plansifter, flowerpot or other some hard holder basins, follow and plant 15～60 days roxburgh anoectochilus terminal bud group of hardening training seedling, every strain spacing is 4～7cm, intensity of illumination is 500～4000lx, relative moisture is 75%～95%, and temperature is 20～30 ℃, cultivates and gathers after 5～7 months.
Adopt rhizoctonia of the present invention (Rhizoctonia spp.) to infect the roxburgh anoectochilus terminal bud tissue cultivating seedling and form symbiosis seedling, can make the roxburgh anoectochilus terminal bud tissue cultivating seedling strengthen resistance, improve survival rate, shorten growth cycle, thereby reduce its production cost, realize industrialization production.
Description of drawings:
Fig. 1 is that rhizoctonia (Rhizoctonia sp.) JSH-1 cultivates 2 days colonial morphology on the PDA medium;
Fig. 2 is the sclerotium form that rhizoctonia (Rhizoctonia sp.) JSH-1 cultivated on the PDA medium 7 days;
Fig. 3 is rhizoctonia (Rhizoctonia sp.) JSH-1 individual morphology and safranin O-KOH cell nucleus dyeing, 400 *;
State when Fig. 4 rigidly connects kind for roxburgh anoectochilus terminal bud;
Fig. 5 is the roxburgh anoectochilus terminal bud contrast seedling growth conditions of having grown 60 days;
Fig. 6 is the anoectochilus symbiosis seedling growth conditions of having grown 60 days;
The hyphal knot that Fig. 7 has withered away in the cortex parenchyma cell for field planting, 100 *;
The hyphal knot that Fig. 8 does not wither away in the cortex parenchyma cell for field planting, 400 *.
Embodiment:
The present invention is further described below in conjunction with specific embodiments and the drawings.
Embodiment one:
Earlier change rhizoctonia (Rhizoctonia sp.) JSH-1 over to the PDA flat board behind the slant tube actication of culture of low-temperature preservation, 28 ℃ of dark culturing two days are standby.
(diameter is 0.5～1cm) or with the mycelia agar block of the transfer needle of calcination block hitting or the picking length of side 0.4～0.7cm on colony edge with sterilized card punch.
Choosing highly is the more sturdy Fujian roxburgh anoectochilus terminal bud group training seedling of 5 ± 0.5cm, receives on the symbiotic culture medium in whole strain mode, and every bottle graft is gone into 3 young plants, and picking rhizoctonia (Rhizoctonia sp.) JSH-1 mycelia agar block places between the 3 strain tissue cultivating seedling; Above-mentioned all operations process is all operated on aseptic super-clean bench.
Condition of culture: temperature: 25 ℃; Intensity of illumination: 1500lx, light application time: 12h/d; Incubation time: 80 days.
Interpretation of result:
1. the roxburgh anoectochilus terminal bud tissue cultivating seedling connects upgrowth situation behind the bacterium
Cultivate the initial stage altogether at tissue cultivating seedling and rhizoctonia (Rhizoctonia sp.) JSH-1, the leaf look turns to be yellow slightly, and plant strain growth is not obvious, and about 20 days, plant base portion stipes begins to occur protocorm.Afterwards, plant strain growth is rapid, and stem is chap gradually, and blade is dark green gradually, and aerial root is more and more flourishing, have in addition be inserted in the medium.And control group, plant strain growth is thin and weak, and here blade some roll up, never the appearance (Fig. 4～6) of protocorm.
2. connect the variation of roxburgh anoectochilus terminal bud tissue cultivating seedling amount of growth (fresh weight and plant height) behind the bacterium
Carry out the mensuration of amount of growth and adopt tissue isolation heavily to separate former bacterial strain with the contrast seedling having cultivated trimestral anoectochilus symbiosis seedling: average plant height before average plant height growth rate (%)=(average plant height before the average plant height in processing back-processing) * 100/ processing; Average fresh weight before average fresh weight growth rate (%)=handle (handling the preceding average fresh weight of the average fresh weight in back-processing) * 100/.The result is as shown in table 1:
Table 1. bacterial strain JSH-1 is to the influence of Fujian roxburgh anoectochilus terminal bud group training growth of seedling situation
(annotate: data are three repetition mean values, adopt the Tukey method in the DPS statistical software, are illustrated in significant difference on 0.01 level between different lowercase marks.)
Embodiment two:
At first make microbial inoculum, optimum preparation method is: rice bran or wheat bran and water are prepared burden according to 1: 1 weight ratio, regulate water content to 50%～60% (possess globule appearance but do not flow down) with PDB with hand-tight, put into sterilized white disk, four layers of gauze of surface coverage, 121 ℃ of sterilization 1h, rhizoctonia (Rhizoctonia sp.) the JSH-1 mycelia agar block of length and width 0.5cm is inserted in the cooling back, cultivated 8 days for dark 28 ℃, rub with the hands broken before using.
The microbial inoculum of making is evenly mixed with cultivation matrix according to 3% weight ratio, and cultivation matrix is the mixture of thin peat and perlite (3: 1).The cultivation matrix that then will be mixed with microbial inoculum places plansifter, and thickness is 3cm, 30 days roxburgh anoectochilus terminal bud tissue cultivating seedling of hardening on the kind, and every strain spacing is 5cm, and intensity of illumination is controlled between 800～3500lx, and relative moisture is 85～90%, and temperature is 22～28 ℃.
Interpretation of result: plant and just observe the appearance that there is protocorm in symbiosis seedling stem after 5 days, protocorm growth rate is up to 93.79% after 27 days.Symbiosis seedling growth during this time is rapid, and new after two months linear leaf is 2～3, and average maximum width of blade is 1.25cm, and the contrast open-birth is long slow, and new linear leaf is 1～2, and average maximum width of blade is 0.85cm.Randomly draw a few strain symbiosis seedlings its protocorm is carried out the free-hand section observation, in its cross section cortex parenchyma cell, all find a large amount of hyphal knots, show the successfully foundation (Fig. 7, Fig. 8) of roxburgh anoectochilus terminal bud-endogenetic fungus syntaxial system.
Embodiment three, embodiment four all test, analyze according to embodiment one, embodiment two described Step By Conditions respectively with rhizoctonia (Rhizoctonia sp.) GXSH-5;
Embodiment five, embodiment six all test, analyze according to embodiment one, embodiment two described Step By Conditions respectively with rhizoctonia (Rhizoctonia sp.) GXSH-6;
Above-mentioned experimental result proves that this two bacterial strain GXSH-5, GXSH-6 can infect the roxburgh anoectochilus terminal bud tissue cultivating seedling equally and form symbiosis seedling, and tangible growth promoting function is arranged.
Enlighten in sum and according to technology of the present invention, adopt rhizoctonia of the present invention (Rhizoctonia sp.) and other rhizoctonias to infect the roxburgh anoectochilus terminal bud tissue cultivating seedling and all can form the mycorhiza symbiosis seedling, all can make the roxburgh anoectochilus terminal bud tissue cultivating seedling strengthen resistance, improve survival rate, shorten growth cycle, thereby reduce its production cost, realize industrialization production.
The rDNA-ITS base sequence of rhizoctonia of the present invention (Rhizoctonia sp.) JSH-1 is as follows:
1 tccgtaggtg?aacctgcgga?aggatcatta?ttgaatttta?atgaggagtt?ggttgttgct
61 ggccttggga?tttttttaac?taattattcc?aggcacttgt?gcacaccttc?tctttcatcc
121?acacacacct?gtgcacttgt?gagacagact?gtggggactt?tattgaaccc?cgtctgtcta
181?cttaatttac?acacactcta?tttaatttaa?actgaatgta?attgatgtaa?cgcatctaat
241?actaagtttc?aacaacggat?ctcttggctc?tcgcatcgat?gaagaacgca?gcgaaatgcg
301?ataagtaatg?tgaattgcag?aattcagtga?atcatcgaat?ctttgaacgc?accttgcgct
361?ccttggtatt?ccttggagca?tgcctgtttg?agtatcatga?aatcttcaaa?gtaaaccttt
421?tgttaattca?atgggttctt?tactttggta?ttggaggtct?ttgcagtctc?acaactgctc
481?ctctttgttc?attagctgga?tctcagtgtt?atgcttggtt?ccactcggcg?tgataagtat
541?ctatcgctga?ggacacctgt?aaaaaggtgg?ccaaggtaaa?tgcagatgaa?ccgcttctaa
601?cagtccattg?acttggacaa?ataaatattt?atgatctgat?ctcaaatcag?gtaggactac
661?ccgctgaact?taagcatatc?aataagcgga?gga
The rDNA-ITS base sequence of described rhizoctonia (Rhizoctonia sp.) GXSH-5 is as follows:
1 tccgtaggtg?aacctgcgga?aggatcatta?atgaattaat?gtggagttgg?ttgtagctgg
61 tccgctggat?tttttaaaat?aaattccttt?ggacatgtgc?acgccttctc?tttcatccac
121?acacacctgt?gcacctgtga?ggcagttgtt?ggggacttta?ttgtaccctc?tctgtctact
181?taatttacac?aaactcaatt?tattttaaaa?cgaatgtaat?tgatgtaacg?catctaatac
241?taagtttcaa?caacggatct?cttggctctc?gcatcgatga?agaacgcagc?gaaatgcgat
301?aagtaatgtg?aattgcagaa?ttcagtgaat?catcgaatct?ttgaacgcac?cttgcgctcc
361?ttggtattcc?ttggagcatg?cctgtttgag?tatcatgaaa?tcttcaaagt?caatcttttt
421?gttaattcaa?ttggttgtgc?tttggtattg?gaggtctttt?gcagcttcac?acctgctcct
481?ctttgttcat?tagctggatc?tcagtgttat?gcttggttcc?actcggcgtg?ataagttatc
541?tatcgctgag?gacacttttt?ttaaaaaaag?gtggccaagg?taaatgcaga?tgaaccgctt
601?ccaatagtcc?atttgcttgg?acaataataa?tttttatgat?ctgatctcaa?atcaggtagg
661?actacccgct?gaacttaagc?atatcaataa?gcggagga
The rDNA-ITS base sequence of described rhizoctonia (Rhizoctonia sp.) GXSH-6 is as follows:
1 tccgtaggtg?aacctgcgga?aggatcatta?atgaatttaa?tgtggagttg?gttgtagctg
61 gtccgctgga?tttctttttt?taaaaaaaat?aaattcctct?gggcatgtgc?acgccttctc
121?tttcatccac?acacacctgt?gcacctgtga?ggcagatgtg?ggcacttgat?ctgtgcccct
181?ctgtctactt?aattcacaca?aactcaattt?atcttaaaat?gaatgtaatt?gatgtaacgc
241?atctaatact?aagtttcaac?aacggatctc?ttggctctcg?catcgatgaa?gaacgcagcg
301?aaatgcgata?agtaatgtga?attgcagaat?tcagtgaatc?atcgaatctt?tgaacgcacc
361?ttgcgctcct?tggtattcct?tggagcatgc?ctgtttgagt?atcatgaaat?cttcaaagtc
421?aatcttttgt?taattcaact?ggttgtgctt?tggtattgga?ggtctttgca?gcttcacacc
481?tgctcctctt?tgttcattag?ctggatctca?gtgttatgct?tggttccact?cggcgtgata
541?agttatctat?cgctgaggac?accgtaaaag?gtggccaagg?taaacacaga?ttgaaccgct
601?tctaatagtc?catttacttg?gacaacatat?ttatgatttg?atctcaaatc?aggtaggact
661?acccgctgaa?cttaagcata?tcaataagcg?gagga
Claims (6)
1. the application of roxburgh anoectochilus terminal bud endogenetic fungus in roxburgh anoectochilus terminal bud is grown seedlings is characterized in that: this endogenetic fungus is rhizoctonia (Rhizoctonia spp.).
2. the application of roxburgh anoectochilus terminal bud endogenetic fungus according to claim 1 in roxburgh anoectochilus terminal bud group cultivation seedling, it is characterized in that: described rhizoctonia (Rhizoctonia spp.) is rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6; These three bacterial strains all have been preserved in China Microbial Culture Preservation Commission common micro-organisms center, and its deposit number and date are respectively JSH-1:CGMCC NO.3074, preservation date on May 20th, 2009; GXSH-5:CGMCC NO.3273, preservation date on September 7th, 2009; GXSH-6:CGMCC NO.3274, preservation date on September 7th, 2009.
3. the application of roxburgh anoectochilus terminal bud endogenetic fungus according to claim 2 in roxburgh anoectochilus terminal bud group cultivation seedling, it is characterized in that: the rDNA-ITS gene order of described rhizoctonia (Rhizoctonia sp.) JSH-1, GXSH-5, GXSH-6 is respectively:
JSH-1：
1 tccgtaggtg?aacctgcgga?aggatcatta?ttgaatttta?atgaggagtt?ggttgttgct
61 ggccttggga?tttttttaac?taattattcc?aggcacttgt?gcacaccttc?tctttcatcc
121?acacacacct?gtgcacttgt?gagacagact?gtggggactt?tattgaaccc?cgtctgtcta
181?cttaatttac?acacactcta?tttaatttaa?actgaatgta?attgatgtaa?cgcatctaat
241?actaagtttc?aacaacggat?ctcttggctc?tcgcatcgat?gaagaacgca?gcgaaatgcg
301?ataagtaatg?tgaattgcag?aattcagtga?atcatcgaat?ctttgaacgc?accttgcgct
361?ccttggtatt?ccttggagca?tgcctgtttg?agtatcatga?aatcttcaaa?gtaaaccttt
421?tgttaattca?atgggttctt?tactttggta?ttggaggtct?ttgcagtctc?acaactgctc
481?ctctttgttc?attagctgga?tctcagtgtt?atgcttggtt?ccactcggcg?tgataagtat
541?ctatcgctga?ggacacctgt?aaaaaggtgg?ccaaggtaaa?tgcagatgaa?ccgcttctaa
601?cagtccattg?acttggacaa?ataaatattt?atgatctgat?ctcaaatcag?gtaggactac
661?ccgctgaact?taagcatatc?aataagcgga?gga
GXSH-5：
1 tccgtaggtg?aacctgcgga?aggatcatta?atgaattaat?gtggagttgg?ttgtagctgg
61 tccgctggat?tttttaaaat?aaattccttt?ggacatgtgc?acgccttctc?tttcatccac
121?acacacctgt?gcacctgtga?ggcagttgtt?ggggacttta?ttgtaccctc?tctgtctact
181?taatttacac?aaactcaatt?tattttaaaa?cgaatgtaat?tgatgtaacg?catctaatac
241?taagtttcaa?caacggatct?cttggctctc?gcatcgatga?agaacgcagc?gaaatgcgat
301?aagtaatgtg?aattgcagaa?ttcagtgaat?catcgaatct?ttgaacgcac?cttgcgctcc
361?ttggtattcc?ttggagcatg?cctgtttgag?tatcatgaaa?tcttcaaagt?caatcttttt
421?gttaattcaa?ttggttgtgc?tttggtattg?gaggtctttt?gcagcttcac?acctgctcct
481?ctttgttcat?tagctggatc?tcagtgttat?gcttggttcc?actcggcgtg?ataagttatc
541?tatcgctgag?gacacttttt?ttaaaaaaag?gtggccaagg?taaatgcaga?tgaaccgctt
601?ccaatagtcc?atttgcttgg?acaataataa?tttttatgat?ctgatctcaa?atcaggtagg
661?actacccgct?gaacttaagc?atatcaataa?gcggagga
GXSH-6：
1 tccgtaggtg?aacctgcgga?aggatcatta?atgaatttaa?tgtggagttg?gttgtagctg
61 gtccgctgga?tttctttttt?taaaaaaaat?aaattcctct?gggcatgtgc?acgccttctc
121?tttcatccac?acacacctgt?gcacctgtga?ggcagatgtg?ggcacttgat?ctgtgcccct
181?ctgtctactt?aattcacaca?aactcaattt?atcttaaaat?gaatgtaatt?gatgtaacgc
241?atctaatact?aagtttcaac?aacggatctc?ttggctctcg?catcgatgaa?gaacgcagcg
301?aaatgcgata?agtaatgtga?attgcagaat?tcagtgaatc?atcgaatctt?tgaacgcacc
361?ttgcgctcct?tggtattcct?tggagcatgc?ctgtttgagt?atcatgaaat?cttcaaagtc
421?aatcttttgt?taattcaact?ggttgtgctt?tggtattgga?ggtctttgca?gcttcacacc
481?tgctcctctt?tgttcattag?ctggatctca?gtgttatgct?tggttccact?cggcgtgata
541?agttatctat?cgctgaggac?accgtaaaag?gtggccaagg?taaacacaga?ttgaaccgct
601?tctaatagtc?catttacttg?gacaacatat?ttatgatttg?atctcaaatc?aggtaggact
661?acccgctgaa?cttaagcata?tcaataagcg gagga
4. the application of roxburgh anoectochilus terminal bud endogenetic fungus according to claim 2 in roxburgh anoectochilus terminal bud group cultivation seedling, it is characterized in that: anoectochilus symbiosis seedling is cultivated in described rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6 and the symbiosis of roxburgh anoectochilus terminal bud tissue cultivating seedling.
5. the application of roxburgh anoectochilus terminal bud endogenetic fungus according to claim 4 in roxburgh anoectochilus terminal bud group cultivation seedling is characterized in that: the mode that described symbiosis is cultivated is for putting in order strain and described rhizoctonia (Rhizoctonia sp.) JSH-1 or GXSH-5 or GXSH-6 co-incubation with roxburgh anoectochilus terminal bud group training seedling.
6. cultivate application in the seedling according to the described roxburgh anoectochilus terminal bud endogenetic fungus of claim 5 in the roxburgh anoectochilus terminal bud group, it is characterized in that: described co-incubation is to carry out in symbiotic culture medium, under the condition that is hedged off from the outer world; Perhaps in cultivation medium, carry out under the open condition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102100732A CN101953261B (en) | 2009-11-04 | 2009-11-04 | Application of jewel orchid endophytic fungi to jewel orchid seedlings |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102100732A CN101953261B (en) | 2009-11-04 | 2009-11-04 | Application of jewel orchid endophytic fungi to jewel orchid seedlings |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101953261A true CN101953261A (en) | 2011-01-26 |
| CN101953261B CN101953261B (en) | 2012-05-30 |
Family
ID=43481060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102100732A Expired - Fee Related CN101953261B (en) | 2009-11-04 | 2009-11-04 | Application of jewel orchid endophytic fungi to jewel orchid seedlings |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101953261B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202227A (en) * | 2013-03-22 | 2013-07-17 | 赖新权 | Seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus |
| CN104137727A (en) * | 2014-07-28 | 2014-11-12 | 厦门加晟生物科技有限公司 | Planting method of anoectochilus formosanus |
| CN113025496A (en) * | 2019-12-24 | 2021-06-25 | 中国医学科学院药用植物研究所 | Fungus for inducing anoectochilus formosanus seeds to germinate into seedlings |
| CN116076344A (en) * | 2022-10-18 | 2023-05-09 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting differentiation, mycorrhizal breeding and cultivation of anoectochilus formosanus protocorm by utilizing rhizoctonia cerealis |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433670A (en) * | 2002-01-25 | 2003-08-06 | 中国医学科学院药用植物研究所 | Application technology of mycorrhizal fungi in Anoectochilus blume cultivation |
| CN100496223C (en) * | 2005-11-07 | 2009-06-10 | 中国医学科学院药用植物研究所 | Application of mycorrhizal fungi in large-scale cultivation of gold thread lotus |
-
2009
- 2009-11-04 CN CN2009102100732A patent/CN101953261B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202227A (en) * | 2013-03-22 | 2013-07-17 | 赖新权 | Seedling breeding and rapid propagation cultivation method for traditional Chinese herbal medicine anoectochilus formosanus |
| CN104137727A (en) * | 2014-07-28 | 2014-11-12 | 厦门加晟生物科技有限公司 | Planting method of anoectochilus formosanus |
| CN104137727B (en) * | 2014-07-28 | 2016-03-30 | 厦门加晟生物科技有限公司 | A kind of implantation methods of roxburgh anoectochilus terminal bud |
| CN113025496A (en) * | 2019-12-24 | 2021-06-25 | 中国医学科学院药用植物研究所 | Fungus for inducing anoectochilus formosanus seeds to germinate into seedlings |
| CN113025496B (en) * | 2019-12-24 | 2022-03-15 | 中国医学科学院药用植物研究所 | Fungus for inducing anoectochilus formosanus seeds to germinate into seedlings |
| CN116076344A (en) * | 2022-10-18 | 2023-05-09 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting differentiation, mycorrhizal breeding and cultivation of anoectochilus formosanus protocorm by utilizing rhizoctonia cerealis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101953261B (en) | 2012-05-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104560827B (en) | A kind of biocontrol actinomycetes bacterial strain for preventing and treating tobacco bacterial wilt and its application | |
| CN101565676A (en) | Three fungus strains for promoting germination of arethusa seeds | |
| CN107058160B (en) | One plant of peanut rhizosphere bacillus amyloliquefaciens and its application | |
| CN102037856B (en) | Simple cordyceps militaris strain rejuvenation method | |
| CN105274030B (en) | A kind of rhizobium and application thereof | |
| CN105062920B (en) | A kind of how chromogenic streptomycete bacterial strain and its application | |
| US20220369648A1 (en) | Endophytic falciphora oryzae fo-r20 and its application | |
| CN104195056B (en) | A kind of two type umbrellas are mould and application in Herba Dendrobii growth-promoting, drought resisting | |
| CN110423707A (en) | Bacillus subtilis strain NH-6 and its application | |
| CN105132293B (en) | A kind of Alternaria tenuissima and its application in dendrobium candidum growth-promoting, drought resisting | |
| CN101914456A (en) | Fungus for promoting orchid seeds to germinate | |
| CN102108339B (en) | Bacillus megaterium with capability of inducing stress resistance of soybeans and application thereof | |
| CN104195054B (en) | Mould and the application in dendrobium candidum growth-promoting and drought resisting of a kind of two type umbrellas | |
| CN101953261B (en) | Application of jewel orchid endophytic fungi to jewel orchid seedlings | |
| CN106520630B (en) | A kind of bacillus megaterium QBJP-F6 and its application | |
| CN109749953B (en) | Bacillus cereus, microbial inoculum and preparation method and application thereof | |
| CN103087947A (en) | Pseudoxanthomonas sp. ZKB4-4 and application thereof | |
| CN105439657B (en) | A kind of preparation method of the special biologic organic fertilizer of resisting repeated stubbles of strawberry | |
| CN104845892A (en) | R.vinctus and application thereof in promoting aquilaria plants to produce agilawood | |
| CN104762216A (en) | Fungus strain capable of resisting salt stress, and breeding method and application thereof | |
| CN101855994B (en) | Production method of anoectochilus symbiosis seedling | |
| CN107475133B (en) | A kind of pale purple purple spore bacterium and its application in promotion bletilla striata growth | |
| CN110713956B (en) | Lysine bacillus S12 and application thereof | |
| CN104403976A (en) | Antagonistic actinomycete for preventing and controlling continuously cropped strawberry root rot and preparation method of biocontrol agent of antagonistic actinomycete | |
| CN101914457B (en) | Fungus for promoting germination of bletilla striata seeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120530 Termination date: 20151104 |
|
| EXPY | Termination of patent right or utility model |