CN105219656A - A kind of Fusarium oxysporum and the application in promotion Growth of Dendrobium candidum thereof - Google Patents
A kind of Fusarium oxysporum and the application in promotion Growth of Dendrobium candidum thereof Download PDFInfo
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- CN105219656A CN105219656A CN201510624601.4A CN201510624601A CN105219656A CN 105219656 A CN105219656 A CN 105219656A CN 201510624601 A CN201510624601 A CN 201510624601A CN 105219656 A CN105219656 A CN 105219656A
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Abstract
The invention discloses a kind of Fusarium oxysporum, does is its Classification And Nomenclature fusarium oxysporum (Fusarium? oxysporum), bacterial strain number is JSNL004, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, does is deposit number CGMCC? No.11310, preservation date is on September 10th, 2015.Fusarium oxysporum JSNL004 of the present invention action effect in raising Growth of Dendrobium candidum speed etc. is remarkable; And can artificial culture, reproduction speed is fast, is convenient to carry out large-scale production, has good development prospect.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of Fusarium oxysporum and the application in Herba Dendrobii is disease-resistant thereof.
Background technology
Herba Dendrobii is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, and research shows, from its seed germination to the healthy growth of seedling, invariably needs the participation of symbiotic effects.Because dendrobium officinale resource is on the verge of exhaustion, at present, the market requirement of Herba Dendrobii is mainly by artificial culture, but artificial growth Growth of Dendrobium candidum speed is slow, and mean yield is low.Mycorhiza is the symbiote with specific modality structure and function that in soil, mycorrhizal fungi and root system of plant are formed, and is the ubiquitous a kind of commensalism of occurring in nature.Orchid is comparatively strong to the dependency of mycorhiza, mycorrhizal fungi and the conditional formation symbiote of root system of plant, physiology is the entirety mutually coordinated, and in the process of common evolutionary, forms specific morphological specificity.The mutual relationship of mycorrhizal fungi and host plant is in most cases mutually beneficial, and mycorrhizal fungi obtains the required nutritive substance of growth in plant materials, and plant is by being combined with symbiotic effects, also can better obtain required nutrient and moisture etc. from soil.Appropriate application mycorhiza resource, improves the resistance against diseases of Herba Dendrobii, reduces the amount of application of chemical pesticide to a certain extent, and this also meets the strategy of sustainable development of agricultural.Therefore, bush mycorrhiza agent correlative study has become an important directions of Herba Dendrobii industry.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of to Herba Dendrobii growth-promoting functions Be very effective ground mycorrhizal fungi---Ascomycotina Fusarium Fusarium oxysporum (Fusariumoxysporum) JSNL004.
The technical problem that the present invention also will solve is to provide above-mentioned fungi and is improving the application in Growth of Dendrobium candidum speed.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of Fusarium oxysporum, its Classification And Nomenclature is fusarium oxysporum (Fusariumoxysporum), bacterial strain number is JSNL004, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), deposit number is CGMCCNo.11310, and preservation date is on September 10th, 2015.Preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101.
Above-mentioned bacterial strains JSNL004 the good plant of upgrowth situation is separated to obtain in August, 2011 in Yueqing City of Zhejiang Province Herba Dendrobii plants district, and this bacterial strain is remarkable to Herba Dendrobii growth-promoting functions.
The colony characteristics of Fusarium oxysporum (Fusariumoxysporum) JSNL004 is as follows:
Morphological observation shows: after cultivating on PDA puts down a rebellion, visual inspection colonial morphology, finds that its mycelium is fine hair shape, and slightly with milk yellow pigment (Figure 1A); Microconidium unit cell, avette, and be born on single raw bottle stalk, bottle of being everlasting stalk top is polymerized to (Figure 1B).Amplification ITS district complete sequence also checks order, and the 16sRNA complete sequence that pcr amplification obtains is as shown in SEQIDNo:1.
Above-mentioned Fusarium oxysporum to the application of Herba Dendrobii growth-promoting functions also within protection scope of the present invention.
Concrete application method is: Fusarium oxysporum (Fusariumoxysporum) JSNL004 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
Wherein, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose is for external source carbon source, be prepared into solid medium, sterilizing, inoculation Fusarium oxysporum (Fusariumoxysporum) JSNL004,27 DEG C, cultivate 25 ~ 30 days under dark, the pH6-8 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
Beneficial effect: compared with prior art, advantage of the present invention is:
(1) Fusarium oxysporum (Fusariumoxysporum) JSNL004 is comparatively remarkable to Herba Dendrobii growth-promoting functions effect.
(2) Fusarium oxysporum (Fusariumoxysporum) JSNL004 culture condition simply, is easily preserved, and is easy to suitability for industrialized production, has good development prospect.
Accompanying drawing explanation
Fig. 1 is the morphological specificity of Herba Dendrobii mycorrhizal fungi JSNL004.
Fig. 2 is the growth-promoting capability analysis of JSNL004 bacterium to Herba Dendrobii.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1:
Mycorrhizal fungi Fusarium oxysporum (Fusariumoxysporum) JSNL004 is prepared into solid fungicide.Concrete preparation method is: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose for external source carbon source, be prepared into solid culture matrix and load in autoclaving bag, sterilizing 20 minutes under the condition of high voltage of 121 DEG C and 101.3 kPas.Aseptically Arbuscular Mycorrhizal Fungi fungi JSNL004 after culture medium cooling, then carries out artificial culture.Concrete culture condition is 26 DEG C, 12h illumination+12h is dark, pH6.5, cultivates 25 ~ 30 days.Cultured microbial inoculum is processed into 1cm
3the block of size, is positioned over the Herba Dendrobii seedling cultivation matrix surface of planting then.Cultivation of dendrobium officinale selects the nutrition pot of bore 15cm, and each nutrition pot cultivates 1 clump of (5 strain) Herba Dendrobii.Select the Herba Dendrobii plant that growing way is basically identical during inoculation, and carry out the mensuration of height of seedling and leading thread.The solid fungicide inoculum size of every clump of seedlings of Dendrobium officinale is 5g.After using microbial inoculum, Relative water content maintains more than 60% 10 day, guarantees that mycelia survives in the substrate.Inoculate latter 6 months, carry out the Mycorrhizal degree detecting of Herba Dendrobii root system, guarantee that mycorrhizal fungi Fusarium oxysporum (Fusariumoxysporum) JSNL004 inoculated is combined with Herba Dendrobii root, define mycorhiza.Inoculate latter 12 months, carry out the growth-promoting effect analysis (table 1, Fig. 2) of Herba Dendrobii Mycorrhizal plant.With non-Arbuscular Mycorrhizal Fungi microbial inoculum, the Herba Dendrobii plant only inoculating equivalent cotton seed hulls bacterium slag is adjoining tree (CK).
Table 1JSNL004 is to the growth-promoting effect of Herba Dendrobii plant
Claims (4)
1. a Fusarium oxysporum, its Classification And Nomenclature is fusarium oxysporum (Fusariumoxysporum), bacterial strain number is JSNL004, now be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is CGMCCNo.11310, and preservation date is on September 10th, 2015.
2. Fusarium oxysporum according to claim 1 is promoting the application in Growth of Dendrobium candidum.
3. application according to claim 2, it is characterized in that: Fusarium oxysporum (Fusariumoxysporum) JSNL004 is prepared into solid fungicide, be placed in Herba Dendrobii seedling cultivation matrix surface, the solid fungicide inoculum size of every clump of Herba Dendrobii seedling is 5g, after using microbial inoculum, Relative water content maintains more than 60% 10 day, to guarantee that mycelia survives in the substrate.
4. application according to claim 3, it is characterized in that, described solid fungicide prepares as follows: with cotton seed hulls bacterium slag be culture medium, ammonium nitrate is external source nitrogenous source, sucrose is for external source carbon source, be prepared into solid medium, sterilizing, inoculation Fusarium oxysporum (Fusariumoxysporum) JSNL004,27 DEG C, cultivate 25 ~ 30 days under dark, the pH6-8 condition of 12h illumination+12h, cultured microbial inoculum is cut into 1cm
3the block of size, for the inoculation of Herba Dendrobii plant bush mycorrhiza agent.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967624A (en) * | 2017-05-19 | 2017-07-21 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum and its application in officinal dendrobium stem plantation |
| CN106978359A (en) * | 2017-05-19 | 2017-07-25 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in the anti-root rot of dendrobium candidum |
| CN106978358A (en) * | 2017-05-19 | 2017-07-25 | 江苏农林职业技术学院 | One plant promotion Growth of Dendrobium candidum Fusarium oxysporum and its application |
| CN107475134A (en) * | 2017-09-30 | 2017-12-15 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in dendrobium candidum southern blight is prevented and treated |
| CN109182144A (en) * | 2018-09-29 | 2019-01-11 | 江苏农林职业技术学院 | The fungal elicitor and the preparation method and application thereof for promoting purple dendrobium seed to sprout |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108342330B (en) * | 2018-05-09 | 2020-08-28 | 中国科学院天津工业生物技术研究所 | Trichoderma longibrachiatum with broad-spectrum antibacterial performance and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
| CN104195055A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
-
2015
- 2015-09-25 CN CN201510624601.4A patent/CN105219656B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101921718A (en) * | 2010-03-16 | 2010-12-22 | 浙江理工大学 | Dendrobium candidum endophyte with growth promotion function and use thereof |
| CN103981102A (en) * | 2014-05-23 | 2014-08-13 | 广西壮族自治区农业科学院微生物研究所 | DSE (Dark septate endophyte) bacterial strain 24L-4 and application thereof in dendrobium officinale production |
| CN104195054A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application of Umbelopsis dimorpha in promoting growth and improving drought resistance of dendrobium officinale |
| CN104195055A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Fusarium oxysporum and application thereof in resisting leaf spot disease of dendrobium officinale |
| CN104195056A (en) * | 2014-09-10 | 2014-12-10 | 江苏农林职业技术学院 | Umbelopsis dimorpha and application thereof in growth promotion and drought resistance of dendrobium officinale |
Non-Patent Citations (1)
| Title |
|---|
| 赵昕梅 等: "铁皮石斛内生真菌的分离鉴定及其促宿主生长作用", 《河南农业科学》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967624A (en) * | 2017-05-19 | 2017-07-21 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum and its application in officinal dendrobium stem plantation |
| CN106978359A (en) * | 2017-05-19 | 2017-07-25 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in the anti-root rot of dendrobium candidum |
| CN106978358A (en) * | 2017-05-19 | 2017-07-25 | 江苏农林职业技术学院 | One plant promotion Growth of Dendrobium candidum Fusarium oxysporum and its application |
| CN106967624B (en) * | 2017-05-19 | 2019-09-10 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum and its application in officinal dendrobium stem plantation |
| CN106978358B (en) * | 2017-05-19 | 2019-09-10 | 江苏农林职业技术学院 | One plant of Fusarium oxysporum for promoting Growth of Dendrobium candidum and its application |
| CN106978359B (en) * | 2017-05-19 | 2019-09-20 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in the anti-root rot of dendrobium candidum |
| CN107475134A (en) * | 2017-09-30 | 2017-12-15 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in dendrobium candidum southern blight is prevented and treated |
| CN107475134B (en) * | 2017-09-30 | 2019-09-20 | 江苏农林职业技术学院 | One plant of Fusarium solani and its application in prevention and treatment dendrobium candidum southern blight |
| CN109182144A (en) * | 2018-09-29 | 2019-01-11 | 江苏农林职业技术学院 | The fungal elicitor and the preparation method and application thereof for promoting purple dendrobium seed to sprout |
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