CN104541979B - A kind of Pleurotus nebrodensis and its cultural method with short production cycle - Google Patents
A kind of Pleurotus nebrodensis and its cultural method with short production cycle Download PDFInfo
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- CN104541979B CN104541979B CN201510006886.5A CN201510006886A CN104541979B CN 104541979 B CN104541979 B CN 104541979B CN 201510006886 A CN201510006886 A CN 201510006886A CN 104541979 B CN104541979 B CN 104541979B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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Abstract
The invention discloses a kind of Pleurotus nebrodensis with short production cycle for belonging to edible mushroom field；Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) bacterial strain middle peasant Bai Ling 2 of the present invention, on December 12nd, 2014 in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, its deposit number is CGMCC No.10192.The invention also discloses the middle peasant Bai Ling cultural methods of No. 2, including parent species breeding, Primary spawn, cultigen culture, management of producing mushroom and harvesting etc..The invention provides a kind of new Pleurotus nebrodensis bacterial strain, the species of Pleurotus nebrodensis is enriched；Secondly, No. 2 cultivation periods of middle peasant Bai Ling of the present invention are short, only 62~72 days, and cultivation bacterial strain more main than existing Pleurotus nebrodensis shortens about 1 month；In addition, the commodity value of the work song entities of middle peasant Bai Ling 2 of the present invention is high, cap is scalloped shaped, and color is pure white, and quality is hard, is adapted to factory culture.
Description
Technical field
The invention belongs to edible mushroom field, and in particular to a kind of Pleurotus nebrodensis with short production cycle, and the Pleurotus nebrodensis cultivation
Culture method.
Background technology
Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis, also known as asafoetida mushroom, the mutation of pleurotus eryngii asafoetide,
The white mutation of Pleurotus eryngii var. nebrodensis, pleurotus eryngii, wing abalone mushroom, beautiful snow Pleurotus ferulae, the white ganoderma lucidum in Western Paradise, the refreshing mushroom in Tianshan Mountains etc.) belong to Pleurotus
(Pleurotus), Chinese scientific name is Pleurotus nebrodensis (or by Classification system Pleurotus eryngii var.tuoliensis
Literal translate and be：Pleurotus eryngii Tuoli mutation).Pleurotus nebrodensis sporophore plumpness is pure white, quality is fine and smooth tasty and refreshing, nutritious, is a kind of pole
For precious large edible bacterium.Pleurotus nebrodensis facultative parasitism is on the basal part of stem and root of half withered Ferula sinkiangensis, wild resource pole
It is rare.
" Pleurotus nebrodensis " title buys asafoetida mushroom bacterium bag from Beijing Jinxin Edible Fungi Co., Ltd. in 1997 from Xinjiang, in north
Wherein literature name is set to Pleurotus nebrodensis by capital large area fruiting success, classification of fungi scholar fourth of the twelve Earthly Branches Mr. morning mist, and Classification system is set to
Pleurotus nebrodensis, and be recorded in《Macrofungi From China》(fourth of the twelve Earthly Branches morning mist is edited, Henan science tech publishing house,
2000).But research later finds that the Pleurotus nebrodensis of China is different from European really Pleurotus nebrodensis,
The Pleurotus nebrodensis scientific name of China should be Pleurotus eryngii var.tuoliensis (.mycoscience such as Kawai,
2008,49:75-79；The plant genetic resources journals such as Huang Chenyang, 2011,12 (5):825-827,823).And obtained authority
International mycology name database (http://www.indexfungorum.org/) recognize, i.e. the scientific name of Pleurotus nebrodensis is
Pleurotus eryngii var.tuoliensis。
Pleurotus nebrodensis and pleurotus eryngii (Pleurotus eryngii var.eryngii) are mutually of the same race on taxology
Different mutation under (Pleurotus eryngii), fructification mouthfeel is close.But, due to being rushed by Pleurotus eryngii industrial cultivation
Hit, Cultivation of Pleurotus nebrodensis amount is fewer and feweri.Causing the main cause of this change has two：Pleurotus nebrodensis biological efficiency only has 40%
~60%, pleurotus eryngii biological efficiency 70~80%；, it is necessary to after-ripening 1~2 month after Pleurotus nebrodensis rod is covered with, therefore cultivation period
It is very long, it is 90~120 days, pleurotus eryngii bacterium rod does not need long-time after-ripening, cultivation period only has 50~60 days.But Pleurotus nebrodensis is sold
Valency is only higher by 10~20% than pleurotus eryngii.Therefore, the pleurotus eryngii that the cycle is short, yield is high turns into the favorite of edible fungus industrial enterprise,
Cycle length, the Pleurotus nebrodensis yielded poorly are gradually eliminated.Change the situation of Cultivation of Pleurotus nebrodensis gradually atrophy, seed selection growth cycle is short
Or the high Pleurotus nebrodensis of yield turns into key, for factory culture, the cycle is short then even more important, and it means workshop
Turnover is fast, power consumption is few, simultaneously because the cycle is short, Compost moisture content loss is less in incubation, and the yield of fruiting then can
It is higher.
The content of the invention
It is a kind of with short production cycle white present invention aims at providing for the shortcoming of existing Cultivation of Pleurotus nebrodensis cycle length
Clever mushroom.
Another object of the present invention is the cultural method for providing above-mentioned Pleurotus nebrodensis.
The purpose of the present invention is realized by following technical solution：
A kind of Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) bacterial strain middle peasant Bai Ling 2 of the present invention,
On December 12nd, 2014, in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, (preservation address was：
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode 100101), its deposit number is
CGMCC No.10192。
Above-mentioned Pleurotus nebrodensis bacterial strain middle peasant Bai Ling 2 cultural method, comprises the following steps：
(1), parent species are bred：By 1:80~100 weight is than ratio by 0.3~0.5cm of diameter middle peasant Bai Ling 2
Strain is inoculated on mother culture media, and 10~12 are cultivated under the conditions of 22~28 DEG C, relative air humidity are 40~60%, lucifuge
My god, obtain No. 2 parent species of middle peasant Bai Ling；
(2), Primary spawn：According to percentage by weight for 3~5% ratio by the middle peasant Bai Ling 2 of gained in step (1)
Parent species are inoculated on cultivation compost, and 28~35 are cultivated under the conditions of 22~28 DEG C, relative air humidity are 40~60%, lucifuge
My god, obtain No. 2 original seeds of middle peasant Bai Ling；
(3), cultigen culture：According to percentage by weight for 3~5% ratio by the middle peasant Bai Ling 2 of gained in step (2)
Number original seed is inoculated on cultivation compost, under the conditions of 22~28 DEG C, relative air humidity are 40~60%, lucifuge culture 28~
35 days, obtain No. 2 cultigens of middle peasant Bai Ling；
(4), cooling inoculation：According to percentage by weight for 5~8% ratio by the middle peasant Bai Ling 2 of gained in step (3)
Cultigen is inoculated on cultivation compost, 22~28 DEG C, relative air humidity be 40~60%, culture 30 under the conditions of lucifuge~
40 days, mycelia covered with compost；
(5), mycelium stimulation：Sack is opened, the mycoderma on charge level is removed with sterilization spatula, pine pricks suitable for reading, then by bacterium bag pine
On bundle, continue to cultivate 3~5 days, until mycelia recovers；
(6), low temperature stimulation：Bacterium bag, which is placed under 1~3 DEG C of low temperature, to stimulate 7~14 days；Or greenhouse temperature is at 0~15 DEG C,
10~15 DEG C of day and night temperature is kept, is stimulated 10~15 days；
(7), management of producing mushroom and harvesting：Occur after former base, in 5~18 DEG C (agriculture formula cultivations) or 12~14 DEG C of (industrial formulas
Cultivation), relative air humidity is 75~85%, the lux of illumination 500~800, well-ventilated, environment CO2≤1000×10-6Bar
Cultivated 20~30 days under part；When fructification cap is sufficiently spread out, but still there is micro, slight crimping, fructification is harvested when spore does not discharge.
Strain described in above-mentioned cultural method step (1) refers to mycelia.
The raw material constituent and its part by weight of mother culture media described in above-mentioned cultural method step (1) be：Go
180~300g of skin potato ball (taking juice), 15~20g of glucose, 0.5~3g of potassium dihydrogen phosphate, 0.5~3g of magnesium sulfate, agar
15~25g, water 1000ml, pH value are natural.
The raw material constituent and its weight of cultivation compost described in above-mentioned cultural method step (2), (3) or (4)
Percentage is：Cotton seed hulls 75~81%, wheat bran 8~15%, corn flour 2~8%, lime 1~3%；Again using material-water ratio as 1：1.8
~2.3 ratio adds water.
The constituent and its percentage by weight of cultivation compost described in above-mentioned cultural method be preferably：Cotton seed hulls
81%, wheat bran 12.5%, corn flour 3.5%, lime 3%, material-water ratio 1：1.86, i.e. water content are 65%.
Temperature described in above-mentioned cultural method step (1), (2), (3) or (4) is preferably 24~26 DEG C, further preferably
For 25 DEG C.
Relative air humidity described in above-mentioned cultural method step (1), (2), (3) or (4) is preferably 60%.
Inoculation method described in above-mentioned cultural method step (4) is first to extract the hollow plastics that bacterium bag center is previously inserted
Rod, cultigen is accessed in the hole extracted and left after hollow plastic stick.
The preparation method of mother culture media described in above-mentioned cultural method step (1), including will proportionally remove the peel horse
Bell potato wedge (diameter about 1cm) is placed in 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice；Add into gained juice
Enter and be less than 400ml water, then add glucose, potassium dihydrogen phosphate, magnesium sulfate, agar, be settled to 1000ml, small fire adds while stirring
Heat, is completely dissolved to above composition, packing test tube (specification is 20mm × 200mm), 121 DEG C of sterilizing 30min；Inclined-plane is put, it is standby.
Agar used can be agar strip or agar powder.
The preparation method of cultivation compost described in above-mentioned cultural method step (2) or (3) is：Proportionally by each original
Expect component mixing, stir；Water is added according still further to material-water ratio, is stirred, bottle/bag is filled, cultivation compost is gone out using high pressure
Bacterium, 150min is kept at 125 DEG C.
The preparation method of cultivation compost described in above-mentioned cultural method step (4) is：Proportionally by each raw material group
Divide mixing, stir；Water is added according still further to material-water ratio, is stirred, cultivating bag uses polypropylene or Polythene Bag, cultivating bag
General 5~6 of thickness, bacterium bag folding footpath 17cm, long 35cm；The cultivation compost prepared is fitted into bag, it is desirable to elastic appropriateness,
Install in the intraoral hole for turning over centre of back pkt., and insert hollow bar, be placed in disinfection basket, that is, sterilized.Typically adopt
With normal-pressure sterilization, out of steamer after upper air, kept for 12~14 hours, be inoculated with after cooling at 100 DEG C.
Middle peasant Bai Ling 2 of the present invention separation domestication process：
In May, 2009, the present inventor's Gobi desert near the brick field of Tacheng area Yumin County, which is collected, colonizes in Xinjiang
The wild Pleurotus nebrodensis sporophore of asafoetide root, mycelia (strain), detection mycelia rDNA-ITS are obtained by fruit body tissue separation
Size and sequence, are identified as Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis), by isolated strain
In 2009-2011 cultivations, fructification good to mushroom shape every year carries out tissue separation, in difcoPDA culture mediums
(constituent of BDdifcoPDA culture mediums is its ratio:BDdifcoPDA powder 39g, distilled water 1000ml) on cultivate, from
In filter out that mycelial growth rate is fast, robust growth, the bacterial strain of bacterium colony rounding, the bacterial strain cultivated as next year.2012、2013
Year experiment in cultivation finds that No. 1433 bacterial strain fruitings performance filtered out for 2011 is excellent, is named as middle peasant Bai Ling 2.
The advantages of the present invention：(1) the invention provides a kind of new Pleurotus nebrodensis bacterial strain, bacterial strain mycelia life
Long speed is fast, covers with after bacterium bag through thermal stimulation with regard to that can form former base；(2) No. 2 cultivation periods of middle peasant Bai Ling of the present invention are short, and it is planted
The training cycle only has 62~72 days, and cultivation bacterial strain more main than existing Pleurotus nebrodensis shortens 1 month or so.And existing miscellaneous No. 13 of China, middle peasant
Wing Bao, KH2, the Cultivation of Pleurotus nebrodensis cycle of middle peasant No. 1 are respectively 90-120 days, more than 120 days, 90-120 days, 100-110 days.
(2) middle peasant Bai Ling 2 of the present invention fructification commodity value is higher, and its cap is scalloped shaped, and color is pure white, and quality is hard, fits
Close factory culture.
Brief description of the drawings：
Fig. 1 middle peasant Bai Ling 2 fructification photo.
Fig. 2 use the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis bacterial strains of primer P4 amplifications.Wherein M is Marker, and 1 is China miscellaneous 13,2
It is KH2 for middle peasant wing Bao, 3,4 be middle peasant No. 1, and 5 be middle peasant Bai Ling 2.
Fig. 3 use the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis bacterial strains of primer P24 amplifications；Wherein M is Marker, and 1 is China miscellaneous 13,
2 be middle peasant wing Bao, and 3 be KH2, and 4 be middle peasant No. 1, and 5 be middle peasant Bai Ling 2.
The IGS2 that Fig. 4 middle peasant Bai Ling 2 is through the restriction enzyme digestion and electrophoresis collection of illustrative plates of Hap II；Wherein 1 is middle peasant No. 1, and 2 be middle peasant Bai Ling 2
Number.
The IGS2 that Fig. 5 middle peasant Bai Ling 2 is through the restriction enzyme digestion and electrophoresis collection of illustrative plates of Rsa I；Wherein 1 is middle peasant No. 1, and 2 be middle peasant Bai Ling 2
Number.
Embodiment：
Below by way of example, the invention will be further described, but the present invention is not limited in any way.
1 middle peasant Bai Ling of embodiment 2 taxonomic identification：
(1) middle peasant Bai Ling 2 morphological feature and taxonomic identification：
No. 2 former base fasciations of middle peasant Bai Ling, white, cap is white when ripe, mussel shape, and quality is hard, 11-14.5 lis of major diameter
Rice, average 13.7 centimetres, 7.6-12.2 centimetres of minor axis, average 11.8 centimetres, 4-7.6 centimetres of thickness is average 6.3 centimetres；Stem
Wilfully, it is white, wait thick substantially, quality is hard, and surface is smooth, stem averagely grows 3.5 centimetres, averagely thick 3.6 centimetres；Lamella is in milky white
Color, there is a small amount of reticulate pattern, marshalling.Cap length-width ratio about 1.2:1, the length-width ratio about 1 of stem:1, the ratio between cap length and stem length
About 3.6:1.Fructification fruiting is neat (see Fig. 1).According to《Macrofungi From China》(fourth of the twelve Earthly Branches morning mist is edited, and Henan science and technology is published
Society, publishes for 2000) photo and individual features of page 66 about Pleurotus nebrodensis describe, middle peasant Bai Ling 2 and Pleurotus nebrodensis form
Feature is consistent, and therefore, middle peasant Bai Ling 2 belongs to Pleurotus nebrodensis (Pleurotus nebrodensis) in classification, the white spirit introduced as technical background
The Classification system change of mushroom, its correct Classification system is Pleurotus eryngii var.tuoliensis.
(2) the ISSR collection of illustrative plates of 4 Pleurotus nebrodensis bacterial strains with being assert by country contrasts identification and analysis
(1), test strain：Control strain：The Pleurotus nebrodensis bacterial strain assert by country only has 4, i.e. China miscellaneous No. 13, middle peasant
Wing Bao, KH2 and middle peasant No. 1；Bacterial strain of the present invention：Middle peasant Bai Ling 2
(2), test method：Using the DNA of bacterial strain as template, ISSR amplifications are carried out by primer of P4 or P24 respectively, ISSR is obtained
Amplified production；Described primer sequence is as follows respectively：
P4：5’-GGATGCAACACACACACAC-3’(SEQ ID No.1)
P24：5’-CACGAGAGAGAGAGAGA-3’(SEQ ID No.2)
ISSR amplification systems are：The μ L of dNTP (2.5mM) 1.5, primer (10 μm of ol) 1 μ L, Ex TaqTMArchaeal dna polymerase
0.5U, 10 × Ex Taq PCR buffer solutions 2 μ L, the μ L of DNA profiling 4, use ddH2O polishings are to 20 μ L；ISSR reaction conditions：94℃
4min；94 DEG C of 50s, 55 DEG C of 50s, 72 DEG C of 2min, totally 35 circulations；72 DEG C of polishing 7min.By amplified production in 1.0% agarose
Electrophoresis detection is carried out on gel, ISSR amplifications are detected.
As a result the ISSR collection of illustrative plates (see Fig. 2) expanded by primer of P4 and the ISSR collection of illustrative plates expanded by primer of P24 (see Fig. 3)
In it can be seen that there is notable difference band, explanation with China miscellaneous 13, middle peasant's wing Bao, KH2, middle peasant No. 1 in middle peasant Bai Ling 2
Middle peasant Bai Ling 2 is a kind of new Pleurotus nebrodensis (Pleurotuseryngii var.tuoliensis) bacterial strain.
(3) using IGS2-RFLP collection of illustrative plates to No. 2 progress identification and analysis of middle peasant Bai Ling
Test strain：Middle peasant Bai Ling 2 and middle peasant No. 1
Test method：(1), DNA is extracted：No. 2 DNA of middle peasant Bai Ling are extracted according to a conventional method.
(2), primer：InvSR1R and 5SRNAR.
InvSR1R：5’-ACTGGCAGAATCAACCAGGTA-3’(SEQ ID No.3)
5SRNAR：5’-ACCGCATCCCGTCTGAT-3’(SEQ ID No.4)
(3), restriction enzyme：HapⅡ、RsaⅠ.
IGS2-PCR amplification systems：Each 2.5 μ L, Ex Taq of dNTP (2.5mM each) 4 μ L, InvSR1R, 5SRNARTM
Archaeal dna polymerase 1.25U, 1 × PCR buffer solution, DNA profiling 25ng uses ddH2O polishings are to 50 μ L.IGS2-RFLP reaction conditions：
94 DEG C of 1min, 60 DEG C of 1min, 72 DEG C of 3min, totally 35 circulations；72 DEG C, 7min.Digestion system：Restriction enzyme (10U/ μ L)
1 μ L, the 1 μ L of μ L, 10 × buffer of PCR primer 6, with water polishing to 10 μ L.It is placed in digestion 4 hours at 37 DEG C.1.2% agarose
Gel electrophoresis, detects endonuclease reaction result under ultraviolet gel imaging system.Data processing analyzes soft using Quantity One
Part.
As a result middle peasant Bai Ling 2 IGS2 through the digestions of Hap II (see Fig. 4) generation size be 609bp, 1087bp,
1487bp, 1675bp 4 fragments；It is 454bp, 644bp, 1051bp, 1640bp through the digestions of Rsa I (see Fig. 5) generation size
4 fragments.Find that middle peasant Bai Ling 2 banding pattern is different from other Pleurotus nebrodensis bacterial strains by contrast, explanation is a kind of new
Pleurotus nebrodensis bacterial strain.
The Pleurotus nebrodensis middle peasant Bai Ling of the present invention of embodiment 22 experiment in cultivation
(1) breeding of parent species：By 1:80 weight than ratio by No. 2 strains of middle peasant Bai Ling of 0.3~0.5cm sizes (
On December 12nd, 2014, in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation, (preservation address was：
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode 100101), its deposit number is
CGMCC No10192) it is inoculated on mother culture media, cultivated 12 days under the conditions of 25 DEG C, relative air humidity 60%, lucifuge,
Test tube slant is covered with, No. 2 parent species of middle peasant Bai Ling are obtained；The preparation method of wherein mother culture media is：By peeled potatoes stripping and slicing
(diameter about 1cm) 200g, is placed in about 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice；Add into gained juice
Enter and be less than 400ml cold water, then add glucose 20g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.5g, agar powder 15g, be settled to 1000ml,
Stirring is to agar powder without conglomeration, and small fire is heated and stirred simultaneously, is completely dissolved to above composition, dispenses test tube, 121 DEG C of sterilizings
30min；Inclined-plane is put, mother culture media is obtained.
(2) Primary spawn：Middle peasant's No. 2 parent species of Bai Ling obtained by step (1) are connect according to percentage by weight 3%-5% ratio
Plant on cultivation compost, cultivated 30 days under the conditions of 25 DEG C, relative air humidity 60%, lucifuge, obtain No. 2 originals of middle peasant Bai Ling
Kind；Described cultivation compost is prepared as follows：According to percentage by weight ratio by cotton seed hulls 81%, wheat bran
12.5%, corn flour 3.5%, lime 3% is mixed, and is stirred；According still further to material-water ratio 1：1.86 part by weight adds water, stirs
Mix uniform, fill bacterium bag, bacterium bag specification is 17cm × 33cm, thick 4 Polypropylene Bag, pack height 10cm, rubber band tying is used
Autoclaving, 150min is kept at 125 DEG C.After bacterium bag sterilizing is finished, spreading for cooling is taken out.
(3) cultigen culture：Middle peasant's No. 2 original seeds of Bai Ling obtained by step (2) are connect for 5% ratio according to percentage by weight
Kind in cultivation compost (its constituent and its same step of ratio (2)), 25 DEG C, relative air humidity 60%, lucifuge bar
Cultivated 28 days under part, obtain No. 2 cultigens of middle peasant Bai Ling.
(4) preparation of compost, is cultivated：With reference to step (2) prepare cultivation compost, pack, bacterium bag specification be 17cm ×
35cm, thick 4 Polypropylene Bag, expect high 16cm or so, and the long 13cm of centre insertion edible mushroom plastic hollow rod uses diameter
The 3.5cm collars and the sealing of supporting tampon lid；Sterilized, kept for 14 hours at 100 DEG C using normal pressure bacterium.
(5), cooling inoculation：Sterilized after cooling chamber is cleaned up, bacterium bag obtained by step (4) is put into cooling chamber to temperature
It is reduced to normal temperature.Aseptically, the edible mushroom hollow plastic stick in bacterium bag is taken out, according to the ratio of percentage by weight 5%,
In the hole that No. 2 cultigens of middle peasant Bai Ling are inoculated into bacterium bag center, sealed with the collar and supporting tampon lid；In 25 DEG C, air
Relative humidity 60%, half-light, well-ventilated, culture covers with bacterium bag in 30 days.
(6), mycelium stimulation：Sack is opened, the mycoderma of 3 square centimeters of sizes of charge level is removed with sterilization spatula, pine pricks suitable for reading, so
Bacterium bag pine is pricked afterwards, continues to cultivate 5 days, until mycelia recovers.
(7), low temperature stimulation：Bacterium bag is put into low temperature stimulation 7 days under the conditions of 1 DEG C of freezer.
(8), management of producing mushroom and harvesting：The bacterium bag that will be handled by step (7), is transferred to mushroom room, controls temperature at 12 DEG C,
Relative air humidity 85%, the lux of illumination 500~800, well-ventilated, environment CO2≤1000×10-6Under the conditions of cultivate 28
My god；When fructification cap is sufficiently spread out, but still there is micro, slight crimping, fructification is harvested when spore does not discharge；From fructification during harvesting
Root is cut off, and is cased after being wrapped up with tin foil.
From the foregoing, No. 2 cultivation periods of middle peasant Bai Ling of the present invention are short, and its cultivation period is only 62-72 days, than existing
Pleurotus nebrodensis main breed shortens 1 month or so, and such as miscellaneous No. 13 of China, middle peasant's wing Bao, KH2, the cultivation period of middle peasant No. 1 are respectively
90-120 days.Secondly, middle peasant Bai Ling 2 of the present invention fructification (see Fig. 1) commodity value is higher, and its cap is scalloped shaped,
Color is pure white, and quality is hard, and stem length only has 3.5cm, bacterial context average thickness 6.2cm, average list mushroom weight 191g or so, equivalent to biology
Efficiency 38% is learned, is adapted to factory culture.
Claims (1)
1. a kind of Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) bacterial strain middle peasant Bai Ling 2, in 2014
December 12, its deposit number was CGMCC in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation
No.10192。
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| CN115093976B (en) * | 2022-06-27 | 2023-05-09 | 山东省农业科学院 | White beech mushroom ZHSjg-byg and cultivation method thereof |
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