CN104541979A - Pleurotus nebrodensis with short production cycle and cultivation method thereof - Google Patents
Pleurotus nebrodensis with short production cycle and cultivation method thereof Download PDFInfo
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- CN104541979A CN104541979A CN201510006886.5A CN201510006886A CN104541979A CN 104541979 A CN104541979 A CN 104541979A CN 201510006886 A CN201510006886 A CN 201510006886A CN 104541979 A CN104541979 A CN 104541979A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
Abstract
The invention discloses pleurotus nebrodensis with short production cycle, and belongs to the field of edible mushrooms. The pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) strain Zhongnong pleurotus nebrodensis No.2 is stored with number of CGMCC No.10192 in China General Microbiological Culture Collection Center on Deceber 12, 2014. The invention also discloses a cultivation method of Zhongnong pleurotus nebrodensis No.2. The method comprises the steps of breeding mother strain; cultivating stock culture; cultivating cultispecies; managing growth of pleurotus nebrodensis; picking. According to the pleurotus nebrodensis with short production cycle, the novel pleurotus nebrodensi strain is provided, and the species of pleurotus nebrodensi are enriched; in addition, the cultivation period of Zhongnong pleurotus nebrodensis No.2 is short and is 62 to 72 days only, which is about 1 months less than that of the existing main cultivating pleurotus nebrodensis strain; the Zhongnong pleurotus nebrodensis No.2 entity is high in commodity value; the cap is scallop shaped, white, hard and suitable for industrial cultivation.
Description
Technical field
The invention belongs to edible mushrooms field, be specifically related to a kind of Pleurotus nebrodensis with short production cycle, and the cultivating method of this Pleurotus nebrodensis.
Background technology
Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis, have another name called asafoetida mushroom, the mutation of pleurotus eryngii asafoetide, Pleurotus eryngii var. nebrodensis, the mutation of pleurotus eryngii white, wing abalone mushroom, beautiful snow Resina Ferulae mushroom, the white glossy ganoderma in Western Paradise, Tianshan Mountains god mushroom etc.) belong to pleurotus (Pleurotus), Chinese formal name used at school is Pleurotus nebrodensis (or be: the mutation of pleurotus eryngii Tuoli) by Classification system Pleurotus eryngii var.tuoliensis literal translation.Pleurotus nebrodensis sporophore plumpness is pure white, quality is fine and smooth tasty and refreshing, nutritious, is a kind of very precious large edible bacterium.Pleurotus nebrodensis facultative parasitism is on the basal part of stem and root of half withered Ferula sinkiangensisK.M.Shen, and wild resource is extremely rare.
" Pleurotus nebrodensis " title buys asafoetida mushroom bacterium bag from Beijing Jinxin Edible Fungi Co., Ltd. in 1997 from Xinjiang, in the success of Beijing big area fruiting, classification of fungi scholar fourth of the twelve Earthly Branches Mr. morning mist wherein literature name will be decided to be Pleurotus nebrodensis, Classification system is decided to be Pleurotus nebrodensis, and (fourth of the twelve Earthly Branches morning mist is edited to be recorded in " Macrofungi From China ", Henan science tech publishing house, 2000).But the Pleurotus nebrodensis that the Pleurotus nebrodensis of the China of research discovery is afterwards real from Europe is different, the Pleurotus nebrodensis formal name used at school of China should be the Pleurotus eryngii var.tuoliensis (.mycoscience such as Kawai, 2008,49:75-79; Huang Chenyang etc. plant genetic resources journal, 2011,12 (5): 825-827,823).And obtained authority international mycology name database (
http:// www.indexfungorum.org/) admit, namely the formal name used at school of Pleurotus nebrodensis is Pleurotus eryngii var.tuoliensis.
Pleurotus nebrodensis is the different mutation under (Pleurotus eryngii) mutually of the same race on taxonomy from Pleurotus eryngii (Pleurotus eryngii var.eryngii), and sporophore mouthfeel is close.But owing to impacting by Pleurotus eryngii industrial cultivation, Cultivation of Pleurotus nebrodensis amount is fewer and feweri.The major cause of this change is caused to have two: Pleurotus nebrodensis biological efficiency only has 40% ~ 60%, Pleurotus eryngii biological efficiency 70 ~ 80%; After Pleurotus nebrodensis rod covers with, need after-ripening 1 ~ 2 month, therefore cultivation period being very long, is 90 ~ 120 days, and Pleurotus eryngii bacterium rod does not need long-time after-ripening, and cultivation period only has 50 ~ 60 days.But Pleurotus nebrodensis price is only higher by 10 ~ 20% than Pleurotus eryngii.Therefore, the Pleurotus eryngii that the cycle is short, output is high becomes the favorite of edible fungus industrial enterprise, and the cycle, Pleurotus nebrodensis that is long, that yield poorly was eliminated gradually.Change the situation of Cultivation of Pleurotus nebrodensis atrophy gradually, the Pleurotus nebrodensis that seed selection growth cycle is short or output is high becomes crucial, for factory culture, cycle is short then even more important, it means that production plant turnover is fast, power consumption is few, simultaneously because the cycle is short, in culturing process, Compost moisture content loss is less, and the output of fruiting then can be higher.
Summary of the invention
For the shortcoming that the existing Cultivation of Pleurotus nebrodensis cycle is long, the object of the invention is to provide a kind of Pleurotus nebrodensis with short production cycle.
Another object of the present invention is the cultivating method providing above-mentioned Pleurotus nebrodensis.
Object of the present invention realizes by following technical solution:
A kind of Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) No. 2, bacterial strain middle peasant Bai Ling of the present invention, on December 12nd, 2014, in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, (preservation address was: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica, postcode 100101), its deposit number is CGMCC No.10192.
The cultivating method of above-mentioned No. 2, Pleurotus nebrodensis bacterial strain middle peasant Bai Ling, comprises the steps:
(1), femalely plant breeding: in the weight ratio ratio of 1:80 ~ 100 by middle peasant Bai Ling No. 2 strain inoculation of diameter 0.3 ~ 0.5cm on mother culture media, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 10 ~ 12 days under lucifuge condition, obtain No. 2, middle peasant Bai Ling mother and plant;
(2), Primary spawn: according to weight percent be 3 ~ 5% ratio No. 2, the middle peasant Bai Ling of gained in step (1) mother planted be inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 2 original seeds;
(3), cultivar is cultivated: according to weight percent be 3 ~ 5% ratio middle peasant Bai Ling No. 2 original seeds of gained in step (2) are inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 2 cultivars;
(4), cooling inoculation: according to weight percent be 5 ~ 8% ratio middle peasant Bai Ling No. 2 cultivars of gained in step (3) are inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 30 ~ 40 days under lucifuge condition, mycelia covers with culture material;
(5), mycelium stimulation: opened by sack, with the mycoderma on sterilization spatula removing charge level, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 3 ~ 5 days, until mycelia recovers;
(6), low temperature stimulation: stimulate 7 ~ 14 days under bacterium bag being placed in 1 ~ 3 DEG C of low temperature; Or greenhouse temperature is 0 ~ 15 DEG C time, keeps day and night temperature 10 ~ 15 DEG C, stimulate 10 ~ 15 days;
(7), management of producing mushroom and gathering: after there is former base, in 5 ~ 18 DEG C (agriculture formula cultivations) or 12 ~ 14 DEG C (industrial formula cultivation), relative air humidity is 75 ~ 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 20 ~ 30 days under condition; When sporophore cap fully launches, but still have slight crimping, sporophore of gathering when spore does not discharge.
Bacterial classification described in above-mentioned cultivating method step (1) refers to mycelia.
Raw material moiety and the part by weight thereof of the mother culture media described in above-mentioned cultivating method step (1) are: peeled potatoes block 180 ~ 300g (getting juice), glucose 15 ~ 20g, potassium primary phosphate 0.5 ~ 3g, magnesium sulfate 0.5 ~ 3g, agar 15 ~ 25g, water 1000ml, pH value nature.
Raw material moiety and the weight percent thereof of above-mentioned cultivating method step (2), (3) or the cultivation culture material described in (4) are: cotton seed hulls 75 ~ 81%, wheat bran 8 ~ 15%, Semen Maydis powder 2 ~ 8%, lime 1 ~ 3%; Be that the ratio of 1:1.8 ~ 2.3 adds water again with material-water ratio.
The moiety of the cultivation culture material described in above-mentioned cultivating method and weight percent thereof are preferably: cotton seed hulls 81%, wheat bran 12.5%, Semen Maydis powder 3.5%, lime 3%, material-water ratio 1:1.86, namely water content is 65%.
Above-mentioned cultivating method step (1), (2), (3) or the temperature described in (4) are preferably 24 ~ 26 DEG C, more preferably 25 DEG C.
Above-mentioned cultivating method step (1), (2), (3) or the relative air humidity described in (4) are preferably 60%.
Inoculation method described in above-mentioned cultivating method step (4) is the hollow plastic stick of first extracting the insertion in advance of bacterium bag central authorities, in the hole stayed after hollow plastic stick is extracted in cultivar access.
The preparation method of the mother culture media described in above-mentioned cultivating method step (1), comprises and proportionally peeled potatoes block (diameter is about 1cm) is placed in 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice; Add in gained juice and be less than 400ml water, then add glucose, potassium primary phosphate, magnesium sulfate, agar, be settled to 1000ml, little fire heating while stirring, dissolve completely to above composition, packing test tube (specification is 20mm × 200mm), 121 DEG C of sterilizing 30min; Pendulum inclined-plane, for subsequent use.Agar used can be agar strip or agar powder.
The preparation method of above-mentioned cultivating method step (2) or the cultivation culture material described in (3) is: proportionally mixed by each feed composition, stir; Add water according to material-water ratio again, stir, dress bottle/bag, cultivation culture material adopts autoclaving, keeps 150min at 125 DEG C.
The preparation method of the cultivation culture material described in above-mentioned cultivating method step (4) is: proportionally mixed by each feed composition, stir; Add water according to material-water ratio again, stir, cultivating bag adopts polypropylene or polyethylene bag, general 5 ~ 6 of cultivating bag thickness, bacterium bag folding footpath 17cm, long 35cm; The cultivation culture material prepared is loaded in bag, requires degree of tightness appropriateness, install in the hole in the middle of the press-in of rear sack varus, and insert hollow bar, put in disinfection basket, namely carry out sterilizing.General employing normal-pressure sterilization, in steamer after air, keeps 12 ~ 14 hours, inoculates after cooling at 100 DEG C.
The separation domestication process of No. 2, middle peasant Bai Ling of the present invention:
In May, 2009, the present inventor's Gobi desert near the brick field of Yumin County, Tacheng area collects the wild Pleurotus nebrodensis sporophore colonizing in Ferula sinkiangensisK.M.Shen root, mycelia (bacterial classification) is obtained by fruit body tissue separation, detect mycelia rDNA-ITS size and sequence, identify that it is Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis), cultivate being separated the bacterial classification obtained in 2009-2011, the annual sporophore good to mushroom shape carries out separate tissue, all in difcoPDA substratum (moiety i.e. its ratio of BDdifcoPDA substratum: BDdifcoPDA powder 39g, distilled water 1000ml) upper cultivation, therefrom filter out mycelial growth rate fast, robust growth, the bacterial strain of bacterium colony rounding, as the bacterial strain of next year cultivation.2012, experiment in cultivation in 2013 finds that No. 1433 bacterial strain fruiting performances filtered out for 2011 are excellent, No. 2, called after middle peasant Bai Ling.
Advantage of the present invention and beneficial effect: (1) the invention provides a kind of new Pleurotus nebrodensis bacterial strain, this bacterial strain mycelial growth rate is fast, just can form former base after covering with bacterium bag through thermal stimulation; (2) middle peasant Bai Ling of the present invention No. 2 cultivation period are short, and its cultivation period only has 62 ~ 72 days, shorten about 1 month than the main cultivation bacterial strain of existing Pleurotus nebrodensis.And existing China mix No. 13, middle peasant's wing Bao, KH2, middle peasant No. 1 the Cultivation of Pleurotus nebrodensis cycle be respectively 90-120 days, more than 120 days, 90-120 days, 100-110 days.(2) the sporophore commodity value of No. 2, middle peasant Bai Ling of the present invention is higher, and its cap is scalloped shaped, and color is pure white, and quality is hard, is applicable to factory culture.
Accompanying drawing illustrates:
Fig. 1. the sporophore photo of No. 2, middle peasant Bai Ling.
Fig. 2. adopt the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis bacterial strains of primer P4 amplification.Wherein M is Marker, and 1 be China assorted 13,2 is middle peasant's wing Bao, and 3 is KH2, and 4 is middle peasant No. 1, and 5 is No. 2, middle peasant Bai Ling.
Fig. 3. adopt the ISSR collection of illustrative plates of 5 Pleurotus nebrodensis bacterial strains of primer P24 amplification; Wherein M is Marker, and 1 be China assorted 13,2 is middle peasant's wing Bao, and 3 is KH2, and 4 is middle peasant No. 1, and 5 is No. 2, middle peasant Bai Ling.
Fig. 4. the IGS2 that No. 2, middle peasant Bai Ling is through Hap II restriction enzyme digestion and electrophoresis collection of illustrative plates; Wherein 1 is middle peasant No. 1, and 2 is No. 2, middle peasant Bai Ling.
Fig. 5. the IGS2 that No. 2, middle peasant Bai Ling is through Rsa I restriction enzyme digestion and electrophoresis collection of illustrative plates; Wherein 1 is middle peasant No. 1, and 2 is No. 2, middle peasant Bai Ling.
Embodiment:
Below by way of example, the invention will be further described, but do not constitute any limitation the present invention.
The taxonomic identification of No. 2, embodiment 1 middle peasant Bai Ling:
(1) morphological specificity of No. 2, middle peasant Bai Ling and taxonomic identification:
The former base cluster of No. 2, middle peasant Bai Ling is raw, white, and time ripe, cap is in white, mussel shape, and quality is hard, major diameter 11-14.5 centimetre, average 13.7 centimetres, minor axis 7.6-12.2 centimetre, average 11.8 centimetres, thickness 4-7.6 centimetre, average 6.3 centimetres; Stem is wilfully, white, and substantially wait thick, quality is hard, smooth surface, and stem on average grows 3.5 centimetres, on average thick 3.6 centimetres; Lamella is creamy white, and has a small amount of reticulate pattern, marshalling.Cap long-width ratio is about 1.2:1, and the long-width ratio of stem is about 1:1, and cap ratio that is long and stem length is about 3.6:1.Sporophore fruiting neat (see Fig. 1).According to " Macrofungi From China ", (fourth of the twelve Earthly Branches morning mist is edited, Henan science tech publishing house, within 2000, publish) the 66th page of photo about Pleurotus nebrodensis and individual features describe, No. 2, middle peasant Bai Ling is consistent with the morphological specificity of Pleurotus nebrodensis, therefore, No. 2, middle peasant Bai Ling belongs to Pleurotus nebrodensis in classification, and as the Classification system change of the Pleurotus nebrodensis that technical background is introduced, its correct Classification system is Pleurotus eryngii var.tuoliensis.
(2) identification and analysis is contrasted with the ISSR collection of illustrative plates of 4 the Pleurotus nebrodensis bacterial strains assert by country
(1), test strain: control strain: the Pleurotus nebrodensis bacterial strain assert by country only has 4, i.e. assorted No. 13 of China, middle peasant's wing Bao, KH2 and middle peasant No. 1; Bacterial strain of the present invention: No. 2, middle peasant Bai Ling
(2), test method: with the DNA of bacterial strain for template, carry out ISSR amplification with P4 or P24 for primer respectively, obtain ISSR amplified production; Described primer sequence is as follows respectively:
P4：5’-GGATGCAACACACACACAC-3’(SEQ ID No.1)
P24：5’-CACGAGAGAGAGAGAGA-3’(SEQ ID No.2)
ISSR amplification system is: dNTP (2.5mM) 1.5 μ L, primer (10 μm of ol) 1 μ L, Ex Taq
tMarchaeal dna polymerase 0.5U, 10 × Ex Taq PCR damping fluid 2 μ L, DNA profiling 4 μ L, uses ddH
2o polishing to 20 μ L; ISSR reaction conditions: 94 DEG C of 4min; 94 DEG C of 50s, 55 DEG C of 50s, 72 DEG C of 2min, totally 35 circulations; 72 DEG C of polishing 7min.Amplified production is carried out electrophoresis detection on 1.0% sepharose, detects ISSR amplification.
Result with P4 be primer amplification ISSR collection of illustrative plates (see Fig. 2) and with P24 be primer amplification ISSR collection of illustrative plates (see Fig. 3) in all can find out that No. 2, middle peasant Bai Ling and China are assorted 13, all there is notable difference band in middle peasant's wing Bao, KH2, middle peasant No. 1, illustrates that No. 2, middle peasant Bai Ling is a kind of new Pleurotus nebrodensis (Pleurotuseryngii var.tuoliensis) bacterial strain.
(3) IGS2-RFLP collection of illustrative plates is utilized to carry out identification and analysis to No. 2, middle peasant Bai Ling
Test strain: No. 2, middle peasant Bai Ling and middle peasant No. 1
Test method: (1), DNA extraction: extract middle peasant Bai Ling No. 2 DNA according to a conventional method.
(2), primer: InvSR1R and 5SRNAR.
InvSR1R：5’-ACTGGCAGAATCAACCAGGTA-3’(SEQ ID No.3)
5SRNAR：5’-ACCGCATCCCGTCTGAT-3’(SEQ ID No.4)
(3), restriction enzyme: Hap II, Rsa I.
IGS2-PCR amplification system: dNTP (2.5mM each) 4 μ L, InvSR1R, 5SRNAR each 2.5 μ L, Ex Taq
tMarchaeal dna polymerase 1.25U, 1 × PCR damping fluid, DNA profiling 25ng, uses ddH
2o polishing to 50 μ L.IGS2-RFLP reaction conditions: 94 DEG C of 1min, 60 DEG C of 1min, 72 DEG C of 3min, totally 35 circulations; 72 DEG C, 7min.Enzyme cuts system: restriction enzyme (10U/ μ L) 1 μ L, PCR primer 6 μ L, 10 × buffer 1 μ L, with water polishing to 10 μ L.At being placed in 37 DEG C, enzyme cuts 4 hours.1.2% agarose gel electrophoresis, detects endonuclease reaction result under ultraviolet gel imaging system.Data processing adopts Quantity One analysis software.
The IGS2 of No. 2, result middle peasant Bai Ling cuts (see Fig. 4) through Hap II enzyme and generates 4 fragments that size is 609bp, 1087bp, 1487bp, 1675bp; Cut (see Fig. 5) through Rsa I enzyme and generate 4 fragments that size is 454bp, 644bp, 1051bp, 1640bp.Find that the banding pattern of No. 2, middle peasant Bai Ling is all different from other Pleurotus nebrodensis bacterial strains through contrast, explanation is a kind of new Pleurotus nebrodensis bacterial strain.
The experiment in cultivation of No. 2, embodiment 2 Pleurotus nebrodensis middle peasant of the present invention Bai Ling
(1) female breeding of planting: ((preservation address was: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms on December 12nd, 2014 by middle peasant Bai Ling No. 2 bacterial classifications of 0.3 ~ 0.5cm size in the weight ratio ratio of 1:80, postcode 100101), its deposit number is CGMCC No10192) be inoculated on mother culture media, 25 DEG C, relative air humidity 60%, to cultivate 12 days under lucifuge condition, cover with test tube slant, obtain No. 2, middle peasant Bai Ling mother and plant; Wherein the preparation method of mother culture media is: by peeled potatoes stripping and slicing (diameter is about 1cm) 200g, be placed in about 600ml water, boiling water boiling 30min, 4 layers of filtered through gauze, taking juice; Add in gained juice and be less than 400ml cold water, then add glucose 20g, potassium primary phosphate 2g, magnesium sulfate 0.5g, agar powder 15g, be settled to 1000ml, be stirred to agar powder without conglomeration, little fire heating is also stirred simultaneously, dissolves completely to above composition, packing test tube, 121 DEG C of sterilizing 30min; Pendulum inclined-plane, obtains mother culture media.
(2) Primary spawn: No. 2, step (1) gained middle peasant Bai Ling mother kind is inoculated on cultivation culture material according to the ratio of weight percent 3%-5%, 25 DEG C, relative air humidity 60%, to cultivate 30 days under lucifuge condition, obtain middle peasant Bai Ling No. 2 original seeds; Described cultivation culture material is prepared as follows: according to weight percent ratio by cotton seed hulls 81%, wheat bran 12.5%, Semen Maydis powder 3.5%, and lime 3% mixes, and stirs; Add water according to the part by weight of material-water ratio 1:1.86 again, stir, dress bacterium bag, bacterium bag specification is 17cm × 33cm, the Polypropylene Bag of thick 4, and pack height 10cm, rubber band tying, adopts autoclaving, keeps 150min at 125 DEG C.After the sterilizing of bacterium bag, take out spreading for cooling.
(3) cultivar is cultivated: according to weight percent be 5% ratio step (2) gained middle peasant Bai Ling No. 2 original seeds are inoculated in cultivation culture material (its moiety and the same step of ratio (2) thereof), 25 DEG C, relative air humidity 60%, to cultivate 28 days under lucifuge condition, obtain middle peasant Bai Ling No. 2 cultivars.
(4) preparation of culture material, is cultivated: prepare cultivation culture material with reference to step (2), pack, bacterium bag specification is 17cm × 35cm, the Polypropylene Bag of thick 4, expect high about 16cm, the middle edible mushrooms plastic hollow rod inserting long 13cm, with the diameter 3.5cm collar and supporting tampon cap seal mouth; Adopt the sterilizing of normal pressure bacterium, keep 14 hours at 100 DEG C.
(5), cooling inoculation: cooling room is cleaned up rear sterilization, step (4) gained bacterium bag is put into cooling room and is reduced to normal temperature to temperature.Aseptically, take out the edible mushrooms hollow plastic stick in bacterium bag, according to the ratio of weight percent 5%, middle peasant Bai Ling No. 2 cultivars are inoculated in the hole of bacterium bag central authorities, with the collar and supporting tampon cap seal mouth; At 25 DEG C, relative air humidity 60%, half-light, well-ventilated, cultivates and covers with bacterium bag in 30 days.
(6), mycelium stimulation: opened by sack, with the mycoderma of sterilization spatula removing charge level 3 square centimeters of sizes, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 5 days, until mycelia recovers.
(7), low temperature stimulation: bacterium bag to be put under freezer 1 DEG C of condition low temperature stimulation 7 days.
(8), management of producing mushroom and gathering: the bacterium bag that will process through step (7), proceeds to mushroom room, control temperature at 12 DEG C, relative air humidity 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 28 days under condition; When sporophore cap fully launches, but still have slight crimping, sporophore of gathering when spore does not discharge; Cut off from sporophore root when gathering, with vanning after tin foil parcel.
From the foregoing, middle peasant Bai Ling of the present invention No. 2 cultivation period are short, and its cultivation period is only 62-72 days, shorten about 1 month than existing Pleurotus nebrodensis main breed, as China mix No. 13, middle peasant's wing Bao, KH2, middle peasant No. 1 cultivation period be respectively 90-120 days.Secondly, sporophore (see Fig. 1) commodity value of No. 2, middle peasant Bai Ling of the present invention is higher, its cap is scalloped shaped, color is pure white, and quality is hard, and stem is long only has 3.5cm, the average thick 6.2cm of bacterial context, heavy about the 191g of average single mushroom, is equivalent to biological efficiency 38%, is applicable to factory culture.
Claims (10)
1. Pleurotus nebrodensis (Pleurotus eryngii var.tuoliensis) No. 2, a bacterial strain middle peasant Bai Ling, on December 12nd, 2014 in the center preservation of China Committee for Culture Collection of Microorganisms's common micro-organisms, its deposit number is CGMCC No.10192.
2. the cultivating method of No. 2, Pleurotus nebrodensis bacterial strain middle peasant Bai Ling according to claim 1, is characterized in that comprising the steps:
(1), in the weight ratio ratio of 1:80 ~ 100 by middle peasant Bai Ling No. 2 strain inoculation of diameter 0.3 ~ 0.5cm on mother culture media, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 10 ~ 12 days under lucifuge condition, obtain No. 2, middle peasant Bai Ling mother and plant;
(2), according to weight percent be 3 ~ 5% ratio No. 2, the middle peasant Bai Ling of gained in step (1) mother planted be inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 2 original seeds;
(3), according to weight percent be 3 ~ 5% ratio middle peasant Bai Ling No. 2 original seeds of gained in step (2) are inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 28 ~ 35 days under lucifuge condition, obtains middle peasant Bai Ling No. 2 cultivars;
(4), according to weight percent be 5 ~ 8% ratio middle peasant Bai Ling No. 2 cultivars of gained in step (3) are inoculated on cultivation culture material, 22 ~ 28 DEG C, relative air humidity is 40 ~ 60%, cultivate 30 ~ 40 days under lucifuge condition, mycelia covers with culture material;
(5), by sack open, with the mycoderma on sterilization spatula removing charge level, pine is pricked suitable for reading, is then pricked by bacterium bag pine, continues cultivation 3 ~ 5 days, until mycelia recovers;
(6) stimulate 7 ~ 14 days under, bacterium bag being placed in 1 ~ 3 DEG C of low temperature; Or greenhouse temperature is 0 ~ 15 DEG C time, keeps day and night temperature 10 ~ 15 DEG C, stimulate 10 ~ 15 days;
(7), there is former base after, be 5 ~ 18 DEG C (agriculture formula cultivations) or 12 ~ 14 DEG C (industrial formula cultivation) in temperature, relative air humidity is 75 ~ 85%, illumination 500 ~ 800 lux, well-ventilated, environment CO
2≤ 1000 × 10
-6cultivate 20 ~ 30 days under condition; When sporophore cap fully launches, but still have slight crimping, sporophore of gathering when spore does not discharge.
3., according to cultivating method according to claim 2, it is characterized in that the bacterial classification described in its step (1) refers to mycelia.
4. according to cultivating method according to claim 2, it is characterized in that raw material moiety and the part by weight thereof of the mother culture media described in its step (1) are: peeled potatoes block 180 ~ 300g, glucose 15 ~ 20g, potassium primary phosphate 0.5 ~ 3g, magnesium sulfate 0.5 ~ 3g, agar 15 ~ 25g, water 1000ml, pH value nature.
5. according to cultivating method according to claim 2, it is characterized in that its step (2), the raw material moiety of (3) or the cultivation culture material described in (4) and weight percent thereof are: cotton seed hulls 75 ~ 81%, wheat bran 8 ~ 15%, Semen Maydis powder 2 ~ 8%, lime 1 ~ 3%; Be that the ratio of 1:1.8 ~ 2.3 adds water again with material-water ratio.
6., according to cultivating method according to claim 5, it is characterized in that the moiety of described cultivation culture material and weight percent thereof are: cotton seed hulls 81%, wheat bran 12.5%, Semen Maydis powder 3.5%, lime 3%, material-water ratio 1:1.86, namely water content is 65%.
7., according to cultivating method according to claim 2, it is characterized in that its step (1), (2), (3) or the temperature described in (4) are 24 ~ 26 DEG C.
8., according to cultivating method according to claim 7, it is characterized in that described temperature is 25 DEG C.
9., according to cultivating method according to claim 2, it is characterized in that its step (1), (2), (3) or the relative air humidity described in (4) are 60%.
10., according to cultivating method according to claim 2, it is characterized in that the inoculation method described in its step (4) is the hollow plastic stick of first extracting the insertion in advance of bacterium bag central authorities, in the hole stayed after hollow plastic stick is extracted in cultivar access.
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| CN115093976A (en) * | 2022-06-27 | 2022-09-23 | 山东省农业科学院 | Hypsizygus marmoreus ZHSjg-byg and cultivation method thereof |
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| CN115093976B (en) * | 2022-06-27 | 2023-05-09 | 山东省农业科学院 | White beech mushroom ZHSjg-byg and cultivation method thereof |
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