CN105106251A - Method for preparing wall-broken ganoderma lucidum spore powder capsules - Google Patents
Method for preparing wall-broken ganoderma lucidum spore powder capsules Download PDFInfo
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- CN105106251A CN105106251A CN201510589788.9A CN201510589788A CN105106251A CN 105106251 A CN105106251 A CN 105106251A CN 201510589788 A CN201510589788 A CN 201510589788A CN 105106251 A CN105106251 A CN 105106251A
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Abstract
The invention discloses a method for preparing wall-broken ganoderma lucidum spore powder capsules. First trichoderma viride is cultivated, obtained metabolites are utilized to process collected ganoderma lucidum spore powder, diluted hydrochloric acid is added and then intermittent oscillation processing is performed, so that the spore wall is loosened, the treated spore powder undergoes washing, drying, mechanical crushing and ultraviolet sterilization, and the wall-broken ganoderma lucidum spore powder capsules are obtained. Compared with the case that the wall-broken rate byconventional physical and mechanical crushing is low and it is difficult to achieve the satisfactory wall-broken effects, the method is simple to operate, the mechanical wall breaking is adopted after treatment, so that the wall-broken rate of obtained ganoderma lucidum spores is over 99% and even reaches 100%, and accordingly nutritional ingredients in the ganoderma lucidum spore powder can be released better to be directly absorbed by the human intestines and stomach.
Description
Technical field
The invention belongs to Medicines and Health Product processing technique field, be specifically related to a kind of preparation method of Ganoderma spore powder with cellular wall broken capsule.
Background technology
Ganoderma is considered as the excellent tonic product of strengthening by means of tonics, invigorating the spleen and replenishing QI by ancient Chinese medicine doctor, and modern medicine research also proves, the multiple effects such as Ganoderma has antioxidation, antiinflammatory, antitumor, protects the liver, immunomodulating.To be hit by a bullet extremely small avette sexual cell---the Ganoderma spore shot out from Ganoderma lamella, owing to having the whole hereditary material of Ganoderma, to be considered to again the elite of having condensed Ganoderma.But Ganoderma spore outer surround by hard chitin fiber, human body is difficult to fully absorb, and directly ediblely easily causes waste, and therefore, the Ganoderma spore after breaking cellular wall is more suitable for human body the intestines and stomach and directly absorbs.
At present, wall-breaking method for Ganoderma spore powder is mainly divided into Mechanical Method, Physical, chemical method and biologic enzymolysis method, although chemical method and biologic enzymolysis method can make the sporoderm-broken rate of Ganoderma spore significantly improve, but its long action time, easily to remain, and after Mechanical Method and Physical process, sporoderm-broken rate of spore is not high, is difficult to reach satisfied effect.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of Ganoderma spore powder with cellular wall broken capsule, it can significantly improve the shell-broken effect of Ganoderma spore powder, the sporoderm-broken rate of gained Ganoderma spore is made to reach more than 99%, even 100%, thus make the nutritional labeling in Ganoderma spore powder discharge and directly to be absorbed by human body the intestines and stomach.
For achieving the above object, the present invention adopts following technical scheme:
A preparation method for Ganoderma spore powder with cellular wall broken capsule, it specifically comprises the steps:
1) in PDA fluid medium, inoculate Hypocrea virens, cultivate 5-8 days in 30-35 DEG C of bottom fermentation, centrifugal, get supernatant;
2) in the spore powder gathered, add its volume 1.2-1.5 water doubly, then add spore powder weight 0.5-0.8% step 1) gained supernatant, place 8-10h after mixing, period slowly stirs once;
3) in step 2) add the dilute hydrochloric acid that mass concentration is 10% in gained feed liquid, at 35-38 DEG C, carry out intermittent oscillation process after mixing, then slow sucking filtration, the spore powder stayed on filter membrane is washed with water 2-3 time;
4) the spore powder dry 5-8h at 50-80 DEG C will cleaned, more dried spore powder is crossed 120-200 mesh sieve after Mechanical Crushing, incapsulate after ultraviolet disinfection, refill bottle closure.
In step 3), every 1000mL feed liquid adds 10-15mL dilute hydrochloric acid.
The process of intermittent oscillation described in step 3) stops 20s, circular treatment 10-20min after vibration 40s.
remarkable advantage of the present invention is:hypocrea virens is one of cellulase-producing the highest active bacterial strain, and it also can produce the various active enzymes such as chitinase, utilizes its metabolite to process Ganoderma spore powder after being cultivated, and can damage the rigid shell of the outer quilt of Ganoderma spore; Spore powder after the process of Hypocrea virens metabolite is added dilute hydrochloric acid and coordinate intermittent oscillation process, the environment that similar human stomach is wriggled can be formed, the active force utilizing it to produce can make conidial cell wall structure loose further, this spore is adopted Mechanical Crushing process further, can effectively improve Mechanical Crushing efficiency, the sporoderm-broken rate of gained Ganoderma spore is made to reach more than 99%, even 100%, thus make the nutritional labeling in Ganoderma spore powder discharge and directly to be absorbed by human body the intestines and stomach, compared with the sporoderm-broken rate of tradition more than 70%, be significantly improved.
Detailed description of the invention
More being convenient to make content of the present invention understand, below in conjunction with detailed description of the invention, technical solutions according to the invention are described further, but the present invention being not limited only to this.
Consisting of of PDA fluid medium used: Rhizoma Solani tuber osi 200g, glucose 20g, distilled water is settled to 100mL.
Embodiment 1
A preparation method for Ganoderma spore powder with cellular wall broken capsule, it specifically comprises the steps:
1) in PDA fluid medium, inoculate Hypocrea virens, cultivate 5 days in 35 DEG C of bottom fermentations, centrifugal, get supernatant;
2) in the spore powder gathered, add the water of its volume 1.5 times, then add spore powder weight 0.5% step 1) gained supernatant, place 8h after mixing, period slowly stirs once;
3) amount of 10mL, mass concentration 10% dilute hydrochloric acid is added by every 1000mL feed liquid, in step 2) dilute hydrochloric acid that adds in gained feed liquid, at 38 DEG C, intermittent oscillation process is carried out after mixing, namely 20s is stopped after vibration 40s, circular treatment 10min, then slow sucking filtration, washes the spore powder stayed on filter membrane with water 3 times;
4) the spore powder dry 5h at 80 DEG C will cleaned, more dried spore powder is crossed 120 mesh sieves after Mechanical Crushing, incapsulate after ultraviolet disinfection, refill bottle closure.
The sporoderm-broken rate of gained Ganoderma spore powder with cellular wall broken is 99.2%.
Embodiment 2
A preparation method for Ganoderma spore powder with cellular wall broken capsule, it specifically comprises the steps:
1) in PDA fluid medium, inoculate Hypocrea virens, cultivate 6 days in 32 DEG C of bottom fermentations, centrifugal, get supernatant;
2) in the spore powder gathered, add the water of its volume 1.3 times, then add spore powder weight 0.6% step 1) gained supernatant, place 9h after mixing, period slowly stirs once;
3) amount of 12mL, mass concentration 10% dilute hydrochloric acid is added by every 1000mL feed liquid, in step 2) dilute hydrochloric acid that adds in gained feed liquid, at 32 DEG C, intermittent oscillation process is carried out after mixing, namely 20s is stopped after vibration 40s, circular treatment 15min, then slow sucking filtration, washes the spore powder stayed on filter membrane with water 3 times;
4) the spore powder dry 6h at 60 DEG C will cleaned, more dried spore powder is crossed 150 mesh sieves after Mechanical Crushing, incapsulate after ultraviolet disinfection, refill bottle closure.
The sporoderm-broken rate of gained Ganoderma spore powder with cellular wall broken is 99.6%.
Embodiment 3
A preparation method for Ganoderma spore powder with cellular wall broken capsule, it specifically comprises the steps:
1) in PDA fluid medium, inoculate Hypocrea virens, cultivate 8 days in 30 DEG C of bottom fermentations, centrifugal, get supernatant;
2) in the spore powder gathered, add the water of its volume 1.2 times, then add spore powder weight 0.8% step 1) gained supernatant, place 10h after mixing, period slowly stirs once;
3) amount of 15mL, mass concentration 10% dilute hydrochloric acid is added by every 1000mL feed liquid, in step 2) dilute hydrochloric acid that adds in gained feed liquid, at 35 DEG C, intermittent oscillation process is carried out after mixing, namely 20s is stopped after vibration 40s, circular treatment 20min, then slow sucking filtration, washes the spore powder stayed on filter membrane with water 2 times;
4) the spore powder dry 5h at 50 DEG C will cleaned, more dried spore powder is crossed 200 mesh sieves after Mechanical Crushing, incapsulate after ultraviolet disinfection, refill bottle closure.
The sporoderm-broken rate of gained Ganoderma spore powder with cellular wall broken is 100%.
1. by embodiment 1-3 gained Ganoderma spore powder with cellular wall broken capsule in 37-40 DEG C, deposit 3 months under relative humidity 75% condition, then carry out moisture, heavy metal and seed borne fungi, the results are shown in Table 1.
Table 1 testing result
From table 1, Ganoderma spore powder with cellular wall broken capsule stable in properties of the present invention, meets state health standards.
2. the Ganoderma spore powder with cellular wall broken directly adopting mechanical activation comminution crusher machine to obtain and the embodiment of the present invention 1 Ganoderma spore powder with cellular wall broken are carried out immune performance test, its method of testing is: by the healthy SD rat 100 of body weight 70-90g, male and female half and half, be divided into 5 groups at random, often organize 20.5 groups of rats are set to respectively blank group, mechanical breaking-wall method matched group (0.667g/kg), low dose group (0.333g/kg), middle dosage group (0.667g/kg), high dose group (1.000g/kg), according to dosage respectively gastric infusion is carried out to rat, once a day, successive administration is after 20 days, measure rat body weight, spleen weight and thymic weight, calculate spleen/weight ratio and thymus/weight ratio, and serum hemolysin is measured.Result of calculation is in table 2.
Table 2 Ganoderma spore powder on the impact of Rats Organs and Tissues/weight ratio, serum hemolysin (
)
From table 2, after process of the present invention, gained Ganoderma spore powder with cellular wall broken and traditional mechanical are pulverized compared with the Ganoderma spore powder with cellular wall broken that obtains, and its immunoregulation effect improves significantly; And after process of the present invention gained Ganoderma spore powder with cellular wall broken under high dose condition to rat still avirulence, product safety of the present invention is described.
3. the Ganoderma spore powder with cellular wall broken directly adopting mechanical activation comminution crusher machine to obtain and embodiment 1 Ganoderma spore powder with cellular wall broken are carried out tumor suppression experiment, its method of testing is: by the healthy SD rat 100 of body weight 70-90g, male and female half and half, be divided into 5 groups at random, often organize 20.5 groups of rats are set to respectively blank group, mechanical breaking-wall method matched group (0.667g/kg), low dose group (0.333g/kg), middle dosage group (0.667g/kg), high dose group (1.000g/kg), according to dosage respectively gastric infusion is carried out to rat, once a day, blank group gives equivalent distilled water, successive administration is after 20 days, inoculation S-180 sarcoma cell, then continues administration 10 days.Respectively at experiment before, within 10 days, record rat body weight before tumor inoculation with after tumor inoculation, the results are shown in Table 3.
Table 3 Ganoderma spore powder on the impact of rat body weight (
)
From table 3, compared with pulverizing with traditional mechanical the Ganoderma spore powder with cellular wall broken that obtains, after process of the present invention, the tumor inhibition effect of gained Ganoderma spore powder with cellular wall broken is remarkable.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (3)
1. a preparation method for Ganoderma spore powder with cellular wall broken capsule, is characterized in that: specifically comprise the steps:
1) in PDA fluid medium, inoculate Hypocrea virens, cultivate 5-8 days in 30-35 DEG C of bottom fermentation, centrifugal, get supernatant;
2) in the spore powder gathered, add its volume 1.2-1.5 water doubly, then add spore powder weight 0.5-0.8% step 1) gained supernatant, place 8-10h after mixing, period slowly stirs once;
3) in step 2) add the dilute hydrochloric acid that mass concentration is 10% in gained feed liquid, at 35-38 DEG C, carry out intermittent oscillation process after mixing, then slow sucking filtration, the spore powder stayed on filter membrane is washed with water 2-3 time;
4) the spore powder dry 5-8h at 50-80 DEG C will cleaned, more dried spore powder is crossed 120-200 mesh sieve after Mechanical Crushing, incapsulate after ultraviolet disinfection, refill bottle closure.
2. the preparation method of Ganoderma spore powder with cellular wall broken capsule according to claim 1, is characterized in that: in step 3), every 1000mL feed liquid adds 10-15mL dilute hydrochloric acid.
3. the preparation method of Ganoderma spore powder with cellular wall broken capsule according to claim 1, is characterized in that: the process of intermittent oscillation described in step 3) stops 20s, circular treatment 10-20min after vibration 40s.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105601071A (en) * | 2016-01-28 | 2016-05-25 | 耿春茂 | Complex enzyme preparation for wall breaking of sludge |
| CN111705093A (en) * | 2020-06-29 | 2020-09-25 | 天津科技大学 | Method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with green trichoderma |
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| CN1302856A (en) * | 2000-12-25 | 2001-07-11 | 中山大学 | Process for extracting intracapsular lipid matter from ganoderma ectosporium |
| EP1092765A3 (en) * | 1999-10-12 | 2004-01-02 | Xin Liu | Germination-activated red Ganoderma lucidum spores and method for producing the same |
| CN104107197A (en) * | 2014-06-18 | 2014-10-22 | 无锡飞凤生物科技有限公司 | Ultralow temperature wall-breaking method of ganoderma lucidum spore powder |
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Patent Citations (3)
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|---|---|---|---|---|
| EP1092765A3 (en) * | 1999-10-12 | 2004-01-02 | Xin Liu | Germination-activated red Ganoderma lucidum spores and method for producing the same |
| CN1302856A (en) * | 2000-12-25 | 2001-07-11 | 中山大学 | Process for extracting intracapsular lipid matter from ganoderma ectosporium |
| CN104107197A (en) * | 2014-06-18 | 2014-10-22 | 无锡飞凤生物科技有限公司 | Ultralow temperature wall-breaking method of ganoderma lucidum spore powder |
Non-Patent Citations (1)
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| 徐小静等主编: "《生物技术原理与实验》", 31 July 2006, 中央民族大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105601071A (en) * | 2016-01-28 | 2016-05-25 | 耿春茂 | Complex enzyme preparation for wall breaking of sludge |
| CN111705093A (en) * | 2020-06-29 | 2020-09-25 | 天津科技大学 | Method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with green trichoderma |
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