CN105106251B - A kind of preparation method of ganoderma spove powder capsule - Google Patents
A kind of preparation method of ganoderma spove powder capsule Download PDFInfo
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- CN105106251B CN105106251B CN201510589788.9A CN201510589788A CN105106251B CN 105106251 B CN105106251 B CN 105106251B CN 201510589788 A CN201510589788 A CN 201510589788A CN 105106251 B CN105106251 B CN 105106251B
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Abstract
The invention discloses a kind of preparation methods of ganoderma spove powder capsule, it first cultivates trichoderma viride, then the lucidum spore powder of collection is handled with gained metabolite, again plus the laggard oscillation treatment of in the ranks having a rest of dilute hydrochloric acid, keep conidial cell wall loose, the ganoderma spove powder capsule is made after washing, drying, Mechanical Crushing, ultraviolet disinfection in conidia powder that treated.It is low with conventional physical, Mechanical Crushing sporoderm-broken rate, the shell-broken effect for being often difficult to reach satisfied is compared, the present invention is easy to operate, use mechanical breaking-wall method again after processing, the sporoderm-broken rate of gained Reishi sporule can be made to reach 99% or more, even 100%, it is directly absorbed by human body stomach to enable the nutritional ingredient in lucidum spore powder preferably to discharge.
Description
Technical field
The invention belongs to Medicines and Health Product processing technique fields, and in particular to a kind of preparation of ganoderma spove powder capsule
Method.
Background technology
Ganoderma lucidum by ancient Chinese medicine doctor be considered as strengthening by means of tonics, tonifying middle-Jiao and Qi excellent tonic product, modern medicine research also turn out, ganoderma lucidum
With a variety of effects such as anti-oxidant, anti-inflammatory, antitumor, liver protection, immunological regulation.What is ejected from glossy ganoderma lamella is extremely small
Avette reproduction cell --- Reishi sporule, due to ganoderma lucidum whole inhereditary material, and be considered as the essence for having agglomerated ganoderma lucidum
China.But Reishi sporule is outer to be surrounded by hard chitin fiber, and human body is difficult to fully absorb, directly edible to easily cause waste,
Therefore, the Reishi sporule after broken wall is more suitable for human body stomach and directly absorbs.
Currently, being broadly divided into Mechanical Method, Physical, chemical method and biological enzymolysis for the wall-breaking method of lucidum spore powder
Method, although chemical method and biologic enzymolysis method can be such that the sporoderm-broken rate of Reishi sporule significantly improves, its long action time, easily residual,
And sporoderm-broken rate of spore is not high after Mechanical Method and Physical processing, it is difficult to achieve the effect that satisfied.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of ganoderma spove powder capsule, are remarkably improved ganoderma lucidum
The shell-broken effect of conidia powder makes the sporoderm-broken rate of gained Reishi sporule reach 99% or more or even 100%, to make lucidum spore powder
In nutritional ingredient can preferably discharge and directly be absorbed by human body stomach.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of preparation method of ganoderma spove powder capsule, specifically comprises the following steps：
1）Trichoderma viride is inoculated in PDA liquid medium, fermented and cultured 5-8 days at 30-35 DEG C, centrifugation takes
Clear liquid；
2）The water of 1.2-1.5 times of its volume is added in the conidia powder gathered, conidia powder weight 0.5- is then added
0.8% step 1）Gained supernatant places 8-10h after mixing, is during which slowly stirred primary；
3）In step 2）Mass concentration is added in gained feed liquid for 10% dilute hydrochloric acid, into the ranks at 35-38 DEG C after mixing
It has a rest oscillation treatment, then slowly filters, the conidia powder that will be left on filter membrane is washed with water 2-3 times；
4）By clean conidia powder at 50-80 DEG C dry 5-8h, then by the conidia powder after drying after Mechanical Crushing mistake
120-200 mesh sieves, and is packed into capsule after ultraviolet disinfection, refills bottle closure.
Step 3）In 10-15mL dilute hydrochloric acid is added per 1000mL feed liquids.
Step 3）Described in intermittent oscillation processing be oscillation 40s after stop 20s, circular treatment 10-20min.
The remarkable advantage of the present invention is：Trichoderma viride is one of highest bacterial strain of cellulase-producing activity, may be used also
The multiple active enzymes such as chitinase are generated, lucidum spore powder is handled using its metabolite after being cultivated, it can be to spirit
The rigid shell of the outer quilt of Ganoderma lucidum spore damages；By trichoderma viride metabolite, treated that conidia powder adds between dilute hydrochloric acid coordinates
It has a rest oscillation treatment, the environment that similar human stomach is wriggled can be formed, active force using its generation can make spore wall construction into one
Step is loose, further Mechanical Crushing is used to handle in this spore, can effectively improve Mechanical Crushing efficiency, makes gained Reishi sporule
Sporoderm-broken rate reaches 99% or more or even 100%, to so that the nutritional ingredient in lucidum spore powder is preferably discharged and by human body intestines
Stomach directly absorbs, and compared with the sporoderm-broken rate of 70% or more tradition, is significantly improved.
Specific implementation mode
In order to make content of the present invention easily facilitate understanding, With reference to embodiment to of the present invention
Technical solution is described further, but the present invention is not limited only to this.
The group of PDA liquid medium used becomes：Potato 200g, glucose 20g, distilled water are settled to 100mL.
Embodiment 1
A kind of preparation method of ganoderma spove powder capsule, specifically comprises the following steps：
1）Trichoderma viride is inoculated in PDA liquid medium, fermented and cultured 5 days at 35 DEG C, centrifugation takes supernatant；
2）The water of 1.5 times of its volume is added in the conidia powder gathered, 0.5% step 1 of conidia powder weight is then added）
Gained supernatant places 8h after mixing, is during which slowly stirred primary；
3）The amount of 10mL, 10% dilute hydrochloric acid of mass concentration is added by every 1000mL feed liquids, in step 2）It is added in gained feed liquid
Dilute hydrochloric acid, intermittent oscillation processing is carried out after mixing at 38 DEG C, that is, stops 20s, circular treatment 10min after vibrating 40s, then
It slowly filters, the conidia powder that will be left on filter membrane is washed with water 3 times；
4）Clean conidia powder is dried into 5h at 80 DEG C, then the conidia powder after drying is crossed into 120 mesh after Mechanical Crushing
It sieves, is packed into capsule after ultraviolet disinfection, refills bottle closure.
The sporoderm-broken rate of gained ganoderma spove powder is 99.2%.
Embodiment 2
A kind of preparation method of ganoderma spove powder capsule, specifically comprises the following steps：
1）Trichoderma viride is inoculated in PDA liquid medium, fermented and cultured 6 days at 32 DEG C, centrifugation takes supernatant；
2）The water of 1.3 times of its volume is added in the conidia powder gathered, 0.6% step 1 of conidia powder weight is then added）
Gained supernatant places 9h after mixing, is during which slowly stirred primary；
3）The amount of 12mL, 10% dilute hydrochloric acid of mass concentration is added by every 1000mL feed liquids, in step 2）It is added in gained feed liquid
Dilute hydrochloric acid, intermittent oscillation processing is carried out after mixing at 32 DEG C, that is, stops 20s, circular treatment 15min after vibrating 40s, then
It slowly filters, the conidia powder that will be left on filter membrane is washed with water 3 times；
4）Clean conidia powder is dried into 6h at 60 DEG C, then the conidia powder after drying is crossed into 150 mesh after Mechanical Crushing
It sieves, is packed into capsule after ultraviolet disinfection, refills bottle closure.
The sporoderm-broken rate of gained ganoderma spove powder is 99.6%.
Embodiment 3
A kind of preparation method of ganoderma spove powder capsule, specifically comprises the following steps：
1）Trichoderma viride is inoculated in PDA liquid medium, fermented and cultured 8 days at 30 DEG C, centrifugation takes supernatant；
2）The water of 1.2 times of its volume is added in the conidia powder gathered, 0.8% step 1 of conidia powder weight is then added）
Gained supernatant places 10h after mixing, is during which slowly stirred primary；
3）The amount of 15mL, 10% dilute hydrochloric acid of mass concentration is added by every 1000mL feed liquids, in step 2）It is added in gained feed liquid
Dilute hydrochloric acid, intermittent oscillation processing is carried out after mixing at 35 DEG C, that is, stops 20s, circular treatment 20min after vibrating 40s, then
It slowly filters, the conidia powder that will be left on filter membrane is washed with water 2 times；
4）Clean conidia powder is dried into 5h at 50 DEG C, then the conidia powder after drying is crossed into 200 mesh after Mechanical Crushing
It sieves, is packed into capsule after ultraviolet disinfection, refills bottle closure.
The sporoderm-broken rate of gained ganoderma spove powder is 100%.
1. ganoderma spove powder capsule obtained by embodiment 1-3 is stored 3 under the conditions of 37-40 DEG C, relative humidity 75%
A month, moisture, heavy metal and seed borne fungi are then carried out, the results are shown in Table 1.
1 testing result of table
By table 1 as it can be seen that ganoderma spove powder capsule property of the present invention is stablized, meet state health standards.
2. by the ganoderma spove powder directly obtained using mechanical crushing crusher machine and 1 broken wall spirit of the embodiment of the present invention
Ganoderma lucidum spore powder carries out immune performance test, and test method is：By weight 70-90g, the healthy SD rat 100 of half male and half female,
It is randomly divided into 5 groups, every group 20.5 groups of rats are set to blank control group, mechanical breaking-wall method control group（0.667g/kg）, it is low
Dosage group（0.333g/kg）, middle dose group（0.667g/kg）, high dose group（1.000g/kg）, according to dosage respectively to rat into
Row gastric infusion, once a day, successive administration is after 20 days, measurement rat body weight, spleen weight and thymic weight, and calculating spleen/
Weight ratio and thymus gland/weight ratio, and serum hemolysin is measured.Result of calculation is shown in Table 2.
Influence of 2 lucidum spore powder of table to Rats Organs and Tissues/weight ratio, serum hemolysin（）
As can be seen from Table 2, the broken wall spirit that gained ganoderma spove powder is crushed with tradition machinery after processed by the invention
Ganoderma lucidum spore powder is compared, and immunoregulation effect improves notable；And rear gained ganoderma spove powder processed by the invention is in high agent
It is still non-toxic to rat under the conditions of amount, illustrate product safety of the present invention.
3. by the ganoderma spove powder directly obtained using mechanical crushing crusher machine and 1 wall-broken ganoderma spore of embodiment
Powder carries out tumor suppression experiment, and test method is：It is random to divide by weight 70-90g, the healthy SD rat 100 of half male and half female
It is 5 groups, every group 20.5 groups of rats are set to blank control group, mechanical breaking-wall method control group（0.667g/kg）, low dose group
（0.333g/kg）, middle dose group（0.667g/kg）, high dose group（1.000g/kg）, gavage according to dosage is carried out to rat respectively
Administration, once a day, blank control group gives equivalent distilled water, and successive administration is inoculated with S-180 sarcoma cells, then after 20 days
Continue administration 10 days.Rat body weight is recorded before experiment, before tumor inoculation with 10 days after tumor inoculation, the results are shown in Table 3.
Influence of 3 lucidum spore powder of table to rat body weight（）
By table 3 as it can be seen that compared with the ganoderma spove powder that tradition machinery crushes, rear gained processed by the invention is broken
The tumor inhibition effect of wall lucidum spore powder is notable.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (1)
1. a kind of preparation method of ganoderma spove powder capsule, it is characterised in that：Specifically comprise the following steps：
1）Trichoderma viride is inoculated in PDA liquid medium, fermented and cultured 5-8 days at 30-35 DEG C, centrifugation takes supernatant；
2）The water of 1.2-1.5 times of its volume is added in the conidia powder gathered, conidia powder weight 0.5-0.8% steps are then added
Rapid 1）Gained supernatant places 8-10h after mixing, is during which slowly stirred primary；
3）In step 2）The dilute hydrochloric acid that mass concentration is 10% is added in gained feed liquid, carrying out interval at 35-38 DEG C after mixing shakes
Processing is swung, is then slowly filtered, the conidia powder that will be left on filter membrane is washed with water 2-3 times；
4）Clean conidia powder is dried into 5-8h at 50-80 DEG C, then the conidia powder after drying is crossed into 120- after Mechanical Crushing
200 mesh sieve, and are packed into capsule after ultraviolet disinfection, refill bottle closure；
Step 3）In 10-15mL dilute hydrochloric acid is added per 1000mL feed liquids；
Step 3）Described in intermittent oscillation processing be oscillation 40s after stop 20s, circular treatment 10-20min.
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| CN105106251B true CN105106251B (en) | 2018-07-24 |
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| CN105601071A (en) * | 2016-01-28 | 2016-05-25 | 耿春茂 | Complex enzyme preparation for wall breaking of sludge |
| CN111705093A (en) * | 2020-06-29 | 2020-09-25 | 天津科技大学 | Method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with green trichoderma |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302856A (en) * | 2000-12-25 | 2001-07-11 | 中山大学 | Process for extracting intracapsular lipid matter from ganoderma ectosporium |
| EP1092765A3 (en) * | 1999-10-12 | 2004-01-02 | Xin Liu | Germination-activated red Ganoderma lucidum spores and method for producing the same |
| CN104107197A (en) * | 2014-06-18 | 2014-10-22 | 无锡飞凤生物科技有限公司 | Ultralow temperature wall-breaking method of ganoderma lucidum spore powder |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1092765A3 (en) * | 1999-10-12 | 2004-01-02 | Xin Liu | Germination-activated red Ganoderma lucidum spores and method for producing the same |
| CN1302856A (en) * | 2000-12-25 | 2001-07-11 | 中山大学 | Process for extracting intracapsular lipid matter from ganoderma ectosporium |
| CN104107197A (en) * | 2014-06-18 | 2014-10-22 | 无锡飞凤生物科技有限公司 | Ultralow temperature wall-breaking method of ganoderma lucidum spore powder |
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