CN111705093A - Method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with green trichoderma - Google Patents
Method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with green trichoderma Download PDFInfo
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- CN111705093A CN111705093A CN202010603398.3A CN202010603398A CN111705093A CN 111705093 A CN111705093 A CN 111705093A CN 202010603398 A CN202010603398 A CN 202010603398A CN 111705093 A CN111705093 A CN 111705093A
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- Prior art keywords
- ganoderma lucidum
- trichoderma viride
- polysaccharide
- fruiting body
- ganoderma
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention belongs to the field of polysaccharide preparation, and particularly relates to a method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with trichoderma viride, which comprises the following steps: pulverizing Ganoderma fruiting body with pulverizer, adding distilled water into Ganoderma fruiting body powder, adding 1-7V% Trichoderma viride fermentation liquid, shake-bed culturing to obtain zymocyte liquid, adding methanol for precipitation, precipitating with ethanol, separating, and removing supernatant to obtain polysaccharide. According to the application, the ganoderma lucidum fruiting body powder is fermented by the trichoderma viride fermentation liquor, and a proper amount of methanol is added for precipitation to obtain the ganoderma lucidum polysaccharide, the result shows that the extraction rate of the polysaccharide prepared by the trichoderma viride fermentation liquor is improved by 4 times, and the total antioxidant capacity result shows that the ganoderma lucidum polysaccharide extracted by the fermentation method has strong antioxidant activity.
Description
Technical Field
The invention belongs to the field of polysaccharide preparation, and particularly relates to a method for preparing polysaccharide by fermenting ganoderma lucidum sporocarp with trichoderma viride.
Background
Ganoderma lucidum, which belongs to the genus Ganoderma, has been widely used as a tonic in China for the past 2000 years to improve health and longevity. Ganoderma lucidum is involved in clinical treatment of various diseases, such as chronic bronchitis, asthma, cardiovascular diseases, neurasthenia, chronic viral hepatitis, male sexual dysfunction, chemotherapy toxicity, anti-tumor, etc. Ganoderma lucidum is rich in various bioactive compounds, such as triterpenes, polysaccharides, organic acids, alkaloids, furans and sterols.
A large number of researches prove that the polysaccharide separated from the ganoderma lucidum has various pharmacological activities of resisting tumors, regulating immunity, reducing blood sugar, reducing blood fat, protecting liver and the like, and the ganoderma lucidum polysaccharide also has various efficacies of resisting radiation, resisting fatigue, improving blood circulation and the like. The ganoderan can effectively remove hydroxyl free radicals and superoxide anions in vivo, and has certain antioxidant capacity. The traditional methods for extracting ganoderma lucidum polysaccharide mainly comprise a hot water extraction method, an acid-base solution extraction method and the like. The hot water extraction method has the disadvantages of low efficiency, long time consumption and easy reduction of polysaccharide activity. At present, researches find that an ultrasonic-assisted extraction method, a microwave-assisted method, an enzyme method and the like are also sequentially applied to the extraction of the ganoderma lucidum polysaccharide. But the requirement for ultrasonic operation is high, and the extraction, separation and purification of polysaccharide are not facilitated.
Disclosure of Invention
The invention aims to provide a method for preparing polysaccharide by fermenting ganoderma lucidum sporocarp with trichoderma viride.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with trichoderma viride comprises the following steps: pulverizing Ganoderma fruiting body with pulverizer, adding distilled water into Ganoderma fruiting body powder, adding 1-7V% Trichoderma viride fermentation liquid, shake-bed culturing to obtain zymocyte liquid, adding methanol for precipitation, precipitating with ethanol, separating, and removing supernatant to obtain polysaccharide.
Preferably, the addition amount of the Trichoderma viride fermentation broth is 5V%.
The shake culture conditions are 25 ℃ and 140r/min shake culture for 36 h.
The ratio of the ganoderma lucidum fruit body powder to the distilled water is 1: 20 (w/v).
Green colourThe colony count of the trichoderma fermentation liquor is 108CFU/m。
The crushed ganoderma lucidum fruit bodies are powder with the grain diameter of 40 meshes.
Compared with the prior art, the invention has the beneficial effects that:
according to the application, the ganoderma lucidum fruiting body powder is fermented by the trichoderma viride fermentation liquor, and a proper amount of methanol is added for precipitation to obtain the ganoderma lucidum polysaccharide, the result shows that the extraction rate of the polysaccharide prepared by the trichoderma viride fermentation liquor is improved by 4 times, and the total antioxidant capacity result shows that the ganoderma lucidum polysaccharide extracted by the fermentation method has strong antioxidant activity.
Drawings
FIGS. 1-2 show respectively
Detailed Description
In order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the following preferred embodiments.
Example 1: a method for preparing polysaccharide by fermenting ganoderma lucidum fruiting bodies with trichoderma viride comprises the following steps: 1): the trichoderma is added with 10mL of sterile water to prepare trichoderma suspension for later use. And (3) taking a 250mL conical flask, filling 60mL PDA culture medium, sterilizing, adding 150 mu L of trichoderma viride suspension, and fermenting in a constant-temperature shaking table at 25 ℃ and 140r/min for 7 d. Centrifuging the fermentation liquor to obtain supernatant, performing microfiltration with 0.22 μm microporous membrane, and removing mycelium and spore to obtain sterile fermentation liquor of Trichoderma viride; the colony count of the Trichoderma viride suspension was 108CFU/ml. The trichoderma used in the invention is purchased from the institute of microbiology (model: 3.15278) of the Chinese academy of sciences. 2) Preparing polysaccharide by fermenting ganoderma lucidum sporocarp with trichoderma viride: pulverizing Ganoderma fruiting body with pulverizer to obtain powder with particle size of 40 meshes, mixing the powder with a weight ratio of 1: adding distilled water into Ganoderma encarpium powder at a ratio of 20(w/v), adding 0-7 v% Trichoderma viride fermentation liquid, shake culturing at 25 deg.C and 140r/min for 36 hr to obtain zymocyte liquid, adding appropriate amount of methanol for precipitation, precipitating with ethanol, separating, and removing supernatant to obtain polysaccharide.
FIGS. 1-2 show the polysaccharide content obtained by adding different amounts of sterile fermentation broth of Trichoderma viride, respectively, and the overall antioxidant properties of the obtained polysaccharides. The results show that compared with the sterile fermentation liquor (0%) without adding the Trichoderma viride, the sterile fermentation liquor with the Trichoderma viride can obviously improve the content of the prepared polysaccharide and the total antioxidant performance of the polysaccharide.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.
Claims (6)
1. A method for preparing polysaccharide by fermenting lucid ganoderma sporocarp with trichoderma viride is characterized by comprising the following steps: pulverizing Ganoderma fruiting body with pulverizer, adding distilled water into Ganoderma fruiting body powder, adding 1-7V% Trichoderma viride fermentation liquid, shake-bed culturing to obtain zymocyte liquid, adding methanol for precipitation, precipitating with ethanol, separating, and removing supernatant to obtain polysaccharide.
2. The method for preparing polysaccharides from fruiting body of Ganoderma lucidum karst fermented with Trichoderma viride as claimed in claim 1, wherein the fermentation broth of Trichoderma viride is added in an amount of 5V%.
3. The method for preparing polysaccharides from fruiting body of Ganoderma lucidum karst fermented with Trichoderma viride as claimed in claim 1, wherein the shake cultivation is carried out at 25 deg.C and 140r/min for 36 h.
4. The method for preparing polysaccharides from fermented ganoderma lucidum fruiting body of trichoderma viride as claimed in claim 1, wherein the ratio of ganoderma lucidum fruiting body powder to distilled water is 1: 20 (w/v).
5. The method for preparing polysaccharides from fruiting body of Ganoderma lucidum karst fermented with Trichoderma viride as claimed in claim 1, wherein the colony count of the fermentation broth of Trichoderma viride is 108CFU/m。
6. The method for preparing polysaccharides from the fruiting body of Ganoderma lucidum karst fermented with Trichoderma viride as claimed in claim 1, wherein the fruiting body of Ganoderma lucidum karst is pulverized into powder of 40 mesh size.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113912744A (en) * | 2021-05-21 | 2022-01-11 | 云南西草资源开发有限公司 | Method for extracting tricholoma matsutake polysaccharide by using thiobacillus ferrooxidans |
| CN113912750A (en) * | 2021-11-11 | 2022-01-11 | 开平健之源保健食品有限公司 | Method for extracting ganoderma lucidum fruiting body polysaccharide through fermentation pretreatment |
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| US20080112966A1 (en) * | 2006-03-23 | 2008-05-15 | Gow Robert T | Extracts and Methods Comprising Ganoderma Species |
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| JP2018104308A (en) * | 2016-12-22 | 2018-07-05 | 生展生物科技股▲分▼有限公司 | Liquid fermented products of ganoderma lucidum for use in improving symptoms of alcoholic liver disease, alcoholic fatty liver, and inflammation |
| CN110396531A (en) * | 2019-08-09 | 2019-11-01 | 上海市农业科学院 | Biofermentation degradation prepares ganoderma active polysaccharide and its analysis method |
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2020
- 2020-06-29 CN CN202010603398.3A patent/CN111705093A/en active Pending
Patent Citations (6)
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| JPH10287584A (en) * | 1997-04-08 | 1998-10-27 | Oubiken:Kk | Physiologically active substance and its production |
| US20080112966A1 (en) * | 2006-03-23 | 2008-05-15 | Gow Robert T | Extracts and Methods Comprising Ganoderma Species |
| CN105106251A (en) * | 2015-09-17 | 2015-12-02 | 福建省幸福生物科技有限公司 | Method for preparing wall-broken ganoderma lucidum spore powder capsules |
| CN106167530A (en) * | 2016-07-08 | 2016-11-30 | 中国科学院上海高等研究院 | A kind of combined-enzyme method extracts the method for ganoderan |
| JP2018104308A (en) * | 2016-12-22 | 2018-07-05 | 生展生物科技股▲分▼有限公司 | Liquid fermented products of ganoderma lucidum for use in improving symptoms of alcoholic liver disease, alcoholic fatty liver, and inflammation |
| CN110396531A (en) * | 2019-08-09 | 2019-11-01 | 上海市农业科学院 | Biofermentation degradation prepares ganoderma active polysaccharide and its analysis method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113912744A (en) * | 2021-05-21 | 2022-01-11 | 云南西草资源开发有限公司 | Method for extracting tricholoma matsutake polysaccharide by using thiobacillus ferrooxidans |
| CN113912750A (en) * | 2021-11-11 | 2022-01-11 | 开平健之源保健食品有限公司 | Method for extracting ganoderma lucidum fruiting body polysaccharide through fermentation pretreatment |
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