CN109182152B - Culture medium for improving active ingredients of cordyceps sinensis mycelia - Google Patents
Culture medium for improving active ingredients of cordyceps sinensis mycelia Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
Abstract
The invention discloses a culture medium for improving active ingredients of cordyceps sinensis mycelia. The culture medium for improving the active ingredients of the cordyceps sinensis mycelia comprises: potato juice, nutrition powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, radix sophorae flavescentis extract and medical stone water. Compared with the prior art, the culture medium for improving the active ingredients of the cordyceps sinensis mycelia provided by the invention can shorten the culture period of cordyceps sinensis, obviously improve the contents of cordyceps polysaccharide, cordycepic acid, cordycepin and adenosine in the cordyceps sinensis mycelia, and has extremely high nutritional value.
Description
Technical Field
The invention relates to a culture medium for improving active ingredients of cordyceps sinensis mycelia.
Background
Cordyceps of the genus Eumycota, Ascomycotina, Pyrenomycetes, Clavicipitales, Clavicipitaceae. Has effects of invigorating vital essence, tonifying deficiency, relieving cough, and eliminating phlegm. Has multiple pharmacological actions of immunoregulation, tumor resistance, leukocyte increasing, blood pressure lowering, anti-aging, fatigue resistance, endocrine regulation, bacteriostasis, virus resistance and the like, and is regarded as a precious traditional Chinese medicine for nourishing and strengthening in the past. It is formed by that cordyceps sinensis fungus of cordyceps of ergotaceae, namely bat moth larvae parasitized in alpine meadow soil by bat moth hirsutella sinensis, the larvae are rigidified, and long rod-shaped stroma is extracted from the stiff worm head end in summer under proper conditions (namely a complex body formed by fruiting bodies of the cordyceps sinensis fungus and stiff worm hypha nuclei (larva corpses)). It is mainly produced in alpine zone and snowy mountain grassland with altitude of 3500-5000m in provinces such as Qinghai, Tibet, Sichuan, Yunnan and Gansu of China. Modern researches show that the artificial cordyceps mycelia and natural cordyceps have similar chemical components, pharmacological effects and clinical effects and have lower toxicity than natural ones, which provides basis for replacing natural cordyceps with artificially cultured cordyceps mycelia.
Hirsutella hepiali Chen et Shen is separated from fresh Cordyceps sinensis, and is approved by Ministry of health of the people's republic of China to be listed in a fungus strain list for health food, is a natural Cordyceps sinensis artificial substitute, and has the functions of tonifying lung and kidney, stopping bleeding and reducing phlegm. Can be used for treating chronic cough, asthma, cough with hemoptysis, sexual impotence, spermatorrhea, and soreness of waist and knees. Modern medicine finds that the traditional Chinese medicine also has the effects of tranquilizing, resisting convulsion, reducing blood pressure, improving myocardial ischemia, resisting aging, improving immunity, bidirectionally regulating human endocrine, resisting rejection and lung cancer, lymphoma and liver cancer, and the medicinal value of the traditional Chinese medicine is more and more important to the pharmaceutical industry at home and abroad. At present, the medicines containing hirsutella hepiali Chen et Shen powder in the market comprise bailing capsules, bailing tablets, bailing granules and bailing chewable tablets. The health food containing hirsutella hepiali Chen et Shen powder comprises hirsutella hepiali Chen et Shen powder, hirsutella hepiali Chen et Shen mycelium capsule, etc. In the prior art, the culture period of cordyceps sinensis mycelia is long, the quality of the cordyceps sinensis mycelia is uneven, the content of active ingredients is low, and the cordyceps sinensis mycelia do not have the effect of natural cordyceps sinensis.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the culture medium for improving the active ingredients of the cordyceps sinensis mycelia, which can obviously improve the contents of cordyceps polysaccharide, cordycepic acid, cordycepin and adenosine in the cordyceps sinensis mycelia and has extremely high nutritional value.
The specific technical scheme is as follows:
a culture medium for improving active ingredients of cordyceps sinensis mycelia comprises the following raw materials: potato juice, nutrition powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate and medical stone water.
According to another technical scheme, the culture medium for improving the active ingredients of the cordyceps sinensis mycelia comprises the following raw materials: potato juice, nutrition powder, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, radix sophorae flavescentis extract and medical stone water.
Preferably, the culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 4-8 parts of potato juice, 2-6 parts of nutrition powder, 1-3 parts of glucose, 0.1-0.5 part of peptone, 0.02-0.08 part of monopotassium phosphate, 0.02-0.06 part of magnesium sulfate, 0.5-1 part of radix sophorae flavescentis extract and 180 parts of medical stone water.
The potato juice is prepared by the following method, wherein the parts are all by weight: peeling potatoes, removing bud eyes, crushing by a crusher to obtain potato residues, mixing 8-12 parts of potato residues with 30-50 parts of water, preserving the heat at 90-100 ℃ for 40-60 minutes, and filtering by using 200-mesh and 300-mesh filter cloth to obtain potato juice.
The nutritional powder is Hericium erinaceus extract and/or egg albumen powder. Preferably, the nutrition powder is a mixture of a hericium erinaceus extract and egg albumen powder, wherein the mass ratio of the hericium erinaceus extract to the egg albumen powder is (1-5): (1-5).
The hericium erinaceus extract is prepared by the following method, wherein the parts are all by weight: drying hericium erinaceus at 40-60 ℃ to constant weight, crushing, sieving with a 60-100-mesh sieve to obtain hericium erinaceus powder, mixing 5-10 parts of hericium erinaceus powder with 150 parts of 100-containing water, stirring for 5-10 minutes at 300 revolutions per minute of 100-containing water, then adding 0.02-0.06 part of acid protease and 0.05-0.1 part of cellulase, adjusting the pH to 3.5-4.5 by 10% hydrochloric acid by mass fraction, stirring for 1-3 hours at 300 revolutions per minute of 100-containing water at 35-45 ℃ to obtain enzymatic hydrolysate, preserving the enzymatic hydrolysate at 90-100 ℃ for 5-10 minutes, then performing vacuum concentration on the supernatant to 1.08-1.12g/mL at 40 ℃ for 10-20 minutes at 5000 revolutions per minute of 3000-containing water, and performing spray drying to obtain the hericium erinaceus extract.
The medical stone water is prepared by the following method, wherein the parts are all by weight: mixing 1 part of Maifanitum with 20-40 parts of water, standing at 20-30 deg.C for 20-30 min, performing ultrasonic treatment for 10-20 min, and filtering with 800 mesh filter cloth to obtain filtrate as Maifanitum water; the ultrasonic treatment conditions are as follows: the ultrasonic temperature is 40-60 ℃, the ultrasonic frequency is 25-35KHz, and the ultrasonic power is 100-.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing radix sophorae flavescentis, sieving with a 20-50-mesh sieve to obtain radix sophorae flavescentis powder, mixing 5-10 parts of radix sophorae flavescentis powder with 60-100 parts of water, adding 0.01-0.1 part of mixed enzyme, adjusting the pH to 5.5-6.5 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 40-50 ℃ for 1-3 hours at 300 revolutions per minute to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 85-95 deg.C under reflux for 60-90 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 40-80 parts of water into the residue, extracting at 85-95 deg.C under reflux for 40-60 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure to density of 1.08-1.12g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
The mixed enzyme is a mixture of 70-80 wt% of cellulase and 20-30 wt% of alpha-amylase. The invention also provides a method for culturing the cordyceps sinensis mycelia by using the cordyceps sinensis mycelia. The method comprises the following specific steps:
a method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: filling a 2000mL conical flask with 800-;
(2) seed tank culture: filling 80-120L of the culture medium for improving the active ingredients of the cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 125 ℃ for 20-30 minutes in 120-30 ℃, cooling to 20-30 ℃, adding the erlenmeyer flask liquid strain culture solution into the fermentation tank, and culturing for 8-12 days under the conditions that the temperature is 18-25 ℃, the stirring speed is 160-200 revolutions per minute, the tank pressure is 0.04-0.06MPa and the ventilation amount of sterile air is (0.5-1) vvm in the fermentation process to obtain a seed tank culture solution;
(3) culturing in a fermentation tank: placing 1000-1300L of the culture medium for improving the active ingredients of the cordyceps sinensis mycelia in a 2000L fermentation tank, sterilizing at 125-120 ℃ for 20-30 minutes, cooling to 20-30 ℃, adding the culture solution in the seeding tank into the fermentation tank, culturing for 8-12 days under the conditions that the temperature is 18-25 ℃, the stirring speed is 160-200 r/min, the tank pressure is 0.04-0.06MPa and the ventilation amount of sterile air is (0.5-1) vvm in the fermentation process to obtain the culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 3000-;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.01-0.03MPa and temperature of 30-40 deg.C to constant weight, pulverizing, and sieving with 60-100 mesh sieve to obtain Cordyceps sinensis mycelia.
The aeration of the sterile air is the volume of sterile air passing through a unit volume of culture medium per minute, such as: culture medium in fermentation tankHas a volume of 3m3Introducing 1.5 m/min3The aeration ratio of (3: 1.5) to 1: 0.5 and an aeration rate of 0.5 vvm.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia, provided by the invention, can shorten the culture period of the cordyceps sinensis mycelia, remarkably improve the contents of cordyceps polysaccharide, cordycepic acid, cordycepin and adenosine in the cordyceps sinensis mycelia, and has extremely high nutritional value.
Detailed Description
In the present invention, all the equipment and materials are commercially available or commonly used in the art, and the methods in the following examples are conventional in the art unless otherwise specified.
Alpha-amylase, provided by Wuhan Baixing Biotech Co., Ltd, with an enzyme activity of 5 ten thousand U/g.
The cellulase is provided by Wuhan Baixing biological science and technology limited company, and the enzyme activity is 5 ten thousand U/g.
Acid protease, provided by Nanning Donghuangdao Biotechnology Ltd, with enzyme activity of 5 ten thousand U/g
The medical stone is provided by Wen county Hongyu active carbon factory, and has a particle size of 100 meshes.
Radix Sophorae Flavescentis, dried root of Sophora flavescens ait. Provided by bozhou Baichun pharmaceuticals, ltd, origin: jilin.
The glucose is food grade glucose provided by Wan Shunxin Biotechnology limited in Hunan.
Peptone, supplied by Weifeng Biotech Ltd, Zheng Zhou.
Monopotassium phosphate, provided by corridor house Peng color Fine chemical Co., Ltd, pharmaceutical grade.
Magnesium sulfate, magnesium sulfate heptahydrate provided by corridor, Peng color, Fine chemistry, Inc., pharmaceutical grade.
Hericium erinaceum, fruiting body of Hericium erinaceum (Bull. exFr.) Pers. of Hydnaceae of Polyporales of Basidiomycetes of fungi. Xiyao county Xiangguang medicinal materials, Inc., origin: yunnan province.
Egg albumen powder provided by Wuhan Baixing biotechnology limited company.
The hirsutella hepiali Chen et Shen strain is purchased from China center for industrial microorganism strain preservation, and the strain number is as follows: 14017.
the crusher adopts a fruit crusher produced by Towei mechanical equipment factory of Jingjiang city, model PS, and the crushing granularity is 5-8 mm.
Determining the content of cordycepin and adenosine: the test is carried out according to the content determination of cordycepin and adenosine in cordyceps militaris cultivated in different culture media, such as Guo Tao, Zhang, and the like.
And (3) determining the content of cordyceps polysaccharide and cordycepic acid: the test is carried out according to the 'influence of different culture solutions on the contents of cordyceps militaris polysaccharide and cordycepic acid' of Zhu Ming Wei, Wen Lu and the like.
Example 1
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate and 150 parts of medical stone water.
The nutritional powder is egg albumen powder.
The potato juice is prepared by the following method, wherein the parts are all by weight: peeling potatoes, removing bud eyes, crushing by using a crusher to obtain potato residues, mixing 10 parts of potato residues with 40 parts of water, preserving heat at 95 ℃ for 50 minutes, and filtering by using 300-mesh filter cloth to obtain potato juice.
The medical stone water is prepared by the following method, wherein the parts are all by weight: mixing 1 part of medical stone and 30 parts of water, standing for 25 minutes at 25 ℃, performing ultrasonic treatment for 15 minutes, and filtering by adopting 800-mesh filter cloth, wherein the filtrate is medical stone water, and the ultrasonic treatment conditions are as follows: the ultrasonic temperature is 50 ℃, the ultrasonic frequency is 30KHz, and the ultrasonic power is 120W.
Comparative example 1
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate and 150 parts of water.
The nutritional powder is egg albumen powder.
The potato juice is prepared by the following method, wherein the parts are all by weight: peeling potatoes, removing bud eyes, crushing by using a crusher to obtain potato residues, mixing 10 parts of potato residues with 40 parts of water, preserving heat at 95 ℃ for 50 minutes, and filtering by using 300-mesh filter cloth to obtain potato juice.
Example 2
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutritional powder is egg albumen powder.
The preparation method of the potato juice is the same as that of example 1.
The preparation method of the medical stone water is the same as that of the example 1.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing sophora flavescens ait, sieving with a 40-mesh sieve to obtain sophora flavescens ait powder, mixing 8 parts of sophora flavescens ait powder with 80 parts of water, adding 0.05 part of mixed enzyme, adjusting the pH to 6.0 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 45 ℃ at 200 rpm for 2 hours to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 90 deg.C under reflux for 80 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 60 parts of water into the residue, extracting at 90 deg.C under reflux for 50 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure at absolute pressure of 0.02MPa and temperature of 50 deg.C to density of 1.10g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
The mixed enzyme is a mixture of 75 wt% of cellulase and 25 wt% of alpha-amylase.
Example 3
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutritional powder is egg albumen powder.
The preparation method of the potato juice is the same as that of example 1.
The preparation method of the medical stone water is the same as that of the example 1.
The preparation method of the sophora flavescens extracting solution comprises the following steps of: crushing radix sophorae flavescentis, sieving with a 40-mesh sieve to obtain radix sophorae flavescentis powder, mixing 8 parts of radix sophorae flavescentis powder with 80 parts of water, performing reflux extraction at 90 ℃ for 80 minutes, filtering with 300-mesh filter cloth to obtain filtrate and filter residue, adding 60 parts of water into the filter residue, performing reflux extraction at 90 ℃ for 50 minutes, filtering with 300-mesh filter cloth, combining the two filtrates, and performing reduced pressure concentration at the absolute pressure of 0.02MPa and the temperature of 50 ℃ until the density of 50 ℃ is 1.10g/mL to obtain the radix sophorae flavescentis extract.
Example 4
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutritional powder is egg albumen powder.
The preparation method of the potato juice is the same as that of example 1.
The preparation method of the medical stone water is the same as that of the example 1.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing sophora flavescens ait, sieving with a 40-mesh sieve to obtain sophora flavescens ait powder, mixing 8 parts of sophora flavescens ait powder with 80 parts of water, adding 0.05 part of cellulase, adjusting the pH to 6.0 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 45 ℃ at 200 rpm for 2 hours to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 90 deg.C under reflux for 80 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 60 parts of water into the residue, extracting at 90 deg.C under reflux for 50 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure at absolute pressure of 0.02MPa and temperature of 50 deg.C to density of 1.10g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
Example 5
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutritional powder is egg albumen powder.
The preparation method of the potato juice is the same as that of example 1.
The preparation method of the medical stone water is the same as that of the example 1.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing sophora flavescens ait, sieving with a 40-mesh sieve to obtain sophora flavescens ait powder, mixing 8 parts of sophora flavescens ait powder with 80 parts of water, adding 0.05 part of alpha-amylase, adjusting the pH to 6.0 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 45 ℃ at 200 rpm for 2 hours to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 90 deg.C under reflux for 80 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 60 parts of water into the residue, extracting at 90 deg.C under reflux for 50 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure at absolute pressure of 0.02MPa and temperature of 50 deg.C to density of 1.10g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
Example 6
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutritional powder is Hericium erinaceus extract.
The hericium erinaceus extract is prepared by the following method, wherein the parts are all by weight: drying hericium erinaceus at 50 ℃ to constant weight, crushing, sieving with a 80-mesh sieve to obtain hericium erinaceus powder, mixing 8 parts of hericium erinaceus powder with 120 parts of water, stirring for 8 minutes at 200 revolutions per minute, adding 0.04 part of acid protease and 0.06 part of cellulase, adjusting the pH to 4.0 by using hydrochloric acid with the mass fraction of 10%, stirring for 2 hours at 40 ℃ at 200 revolutions per minute to obtain an enzymatic hydrolysate, preserving the temperature of the enzymatic hydrolysate at 95 ℃ for 6 minutes, separating the core at 4000 revolutions per minute for 20 minutes, concentrating the supernatant at the absolute pressure of 0.02MPa and the temperature of 40 ℃ under reduced pressure until the density of 40 ℃ is 1.10g/mL, and performing spray drying to obtain a hericium erinaceus extract, wherein the spray drying conditions are as follows: the air inlet temperature is 170 ℃, and the air outlet temperature is 90 ℃.
The potato juice is prepared by the following method, wherein the parts are all by weight: peeling potatoes, removing bud eyes, crushing by using a crusher to obtain potato residues, mixing 10 parts of potato residues with 40 parts of water, preserving heat at 95 ℃ for 50 minutes, and filtering by using 300-mesh filter cloth to obtain potato juice.
The medical stone water is prepared by the following method, wherein the parts are all by weight: mixing 1 part of medical stone and 30 parts of water, standing for 25 minutes at 25 ℃, performing ultrasonic treatment for 15 minutes, and filtering by adopting 800-mesh filter cloth, wherein the filtrate is medical stone water, and the ultrasonic treatment conditions are as follows: the ultrasonic temperature is 50 ℃, the ultrasonic frequency is 30KHz, and the ultrasonic power is 120W.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing sophora flavescens ait, sieving with a 40-mesh sieve to obtain sophora flavescens ait powder, mixing 8 parts of sophora flavescens ait powder with 80 parts of water, adding 0.05 part of mixed enzyme, adjusting the pH to 6.0 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 45 ℃ at 200 rpm for 2 hours to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 90 deg.C under reflux for 80 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 60 parts of water into the residue, extracting at 90 deg.C under reflux for 50 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure at absolute pressure of 0.02MPa and temperature of 50 deg.C to density of 1.10g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
The mixed enzyme is a mixture of 75 wt% of cellulase and 25 wt% of alpha-amylase.
The test result of the content of the active ingredients of the obtained cordyceps sinensis mycelia comprises the following steps: 65.35mg/g of cordyceps polysaccharide, 163.21 mg/g of cordycepic acid, 7.36mg/g of cordycepin and 10.17mg/g of adenosine.
Example 7
A method for preparing Cordyceps sinensis mycelia comprises the following steps:
(1) inoculating and culturing: putting 1000mL of culture medium for improving active ingredients of cordyceps sinensis mycelia into 2000mL of conical bottles, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, inoculating hirsutella hepiali Chen et Shen strains under an aseptic condition, and culturing on a constant-temperature shaking table at 20 ℃ until the concentration of mycelia is 32 wt% and the rotation speed of the shaking table is 160 r/min to obtain a conical bottle liquid strain culture solution;
(2) seed tank culture: loading 100L of culture medium for improving active ingredients of Cordyceps sinensis mycelia into a 200L fermentation tank, sterilizing at 121 deg.C for 30 min, cooling to 25 deg.C, adding conical flask liquid strain culture solution into the fermentation tank, culturing at 20 deg.C and stirring speed of 180 r/min for 10 days under the conditions of tank pressure of 0.05MPa and ventilation of sterile air of 0.6vvm during fermentation to obtain seed tank culture solution;
(3) culturing in a fermentation tank: putting 1200L of culture medium for improving active ingredients of cordyceps sinensis mycelia into a 2000L fermentation tank, sterilizing at 121 ℃ for 30 minutes, cooling to 25 ℃, adding culture solution in a seeding tank into the fermentation tank, and culturing for 10 days at 20 ℃ and a stirring speed of 180 r/min, wherein the tank pressure is 0.05MPa and the ventilation quantity of sterile air is 0.6vvm in the fermentation process to obtain culture solution in the fermentation tank;
(4) centrifuging: centrifuging the culture solution in the fermentation tank at 4000 rpm for 30 minutes, and removing the supernatant to obtain a precipitate;
(5) ultra-low temperature vacuum drying: drying the precipitate at absolute pressure of 0.02MPa and temperature of 35 deg.C to constant weight, pulverizing, and sieving with 80 mesh sieve to obtain Cordyceps sinensis mycelia.
The culture medium for improving the active ingredients of the cordyceps sinensis mycelia is prepared by mixing the following raw materials in parts by weight: 6 parts of potato juice, 4 parts of nutrition powder, 2 parts of glucose, 0.2 part of peptone, 0.06 part of monopotassium phosphate, 0.04 part of magnesium sulfate, 0.6 part of radix sophorae flavescentis extract and 150 parts of medical stone water.
The nutrition powder is a mixture of a hericium erinaceus extract and egg albumen powder, wherein the mass ratio of the hericium erinaceus extract to the egg albumen powder is 3:1.
the hericium erinaceus extract is prepared by the following method, wherein the parts are all by weight: drying hericium erinaceus at 50 ℃ to constant weight, crushing, sieving with a 80-mesh sieve to obtain hericium erinaceus powder, mixing 8 parts of hericium erinaceus powder with 120 parts of water, stirring for 8 minutes at 200 revolutions per minute, adding 0.04 part of acid protease and 0.06 part of cellulase, adjusting the pH to 4.0 by using hydrochloric acid with the mass fraction of 10%, stirring for 2 hours at 40 ℃ at 200 revolutions per minute to obtain an enzymatic hydrolysate, preserving the temperature of the enzymatic hydrolysate at 95 ℃ for 6 minutes, separating the core at 4000 revolutions per minute for 20 minutes, concentrating the supernatant at the absolute pressure of 0.02MPa and the temperature of 40 ℃ under reduced pressure until the density of 40 ℃ is 1.10g/mL, and performing spray drying to obtain a hericium erinaceus extract, wherein the spray drying conditions are as follows: the air inlet temperature is 170 ℃, and the air outlet temperature is 90 ℃.
The potato juice is prepared by the following method, wherein the parts are all by weight: peeling potatoes, removing bud eyes, crushing by using a crusher to obtain potato residues, mixing 10 parts of potato residues with 40 parts of water, preserving heat at 95 ℃ for 50 minutes, and filtering by using 300-mesh filter cloth to obtain potato juice.
The medical stone water is prepared by the following method, wherein the parts are all by weight: mixing 1 part of medical stone and 30 parts of water, standing for 25 minutes at 25 ℃, performing ultrasonic treatment for 15 minutes, and filtering by adopting 800-mesh filter cloth, wherein the filtrate is medical stone water, and the ultrasonic treatment conditions are as follows: the ultrasonic temperature is 50 ℃, the ultrasonic frequency is 30KHz, and the ultrasonic power is 120W.
The preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing sophora flavescens ait, sieving with a 40-mesh sieve to obtain sophora flavescens ait powder, mixing 8 parts of sophora flavescens ait powder with 80 parts of water, adding 0.05 part of mixed enzyme, adjusting the pH to 6.0 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 45 ℃ at 200 rpm for 2 hours to obtain an enzymatic hydrolysate;
(2) extracting the enzymolysis solution at 90 deg.C under reflux for 80 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 60 parts of water into the residue, extracting at 90 deg.C under reflux for 50 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure at absolute pressure of 0.02MPa and temperature of 50 deg.C to density of 1.10g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
The mixed enzyme is a mixture of 75 wt% of cellulase and 25 wt% of alpha-amylase.
The test result of the content of the active ingredients of the obtained cordyceps sinensis mycelia comprises the following steps: 79.45mg/g of cordyceps polysaccharide, 186.64 mg/g of cordycepic acid, 8.41mg/g of cordycepin and 12.36mg/g of adenosine.
Test example 1
The contents of cordyceps polysaccharide and cordycepic acid in the cordyceps sinensis mycelia obtained in the embodiment are tested, and specific test results are shown in table 1.
TABLE 1 Cordyceps sinensis mycelia, Cordyceps polysaccharide and cordycepic acid content test results table
Test example 2
The contents of cordycepin and adenosine in the cordyceps sinensis mycelia obtained in the examples were tested, and the specific test results are shown in table 2.
TABLE 2 Cordyceps sinensis mycelia Cordycepin and adenosine content test results table
| Cordycepin, mg/g | Adenosine, mg/g | |
| Example 1 | 6.25 | 8.27 |
| Comparative example 1 | 4.82 | 7.11 |
| Example 2 | 7.28 | 9.96 |
| Example 3 | 6.47 | 8.74 |
| Example 4 | 6.73 | 9.01 |
| Example 5 | 6.58 | 8.92 |
In the embodiment 1, the medical stone water containing a plurality of trace elements can obviously promote the growth of the cordyceps sinensis mycelia and improve the content of various beneficial components. In example 2, the sophora flavescens ait is added, and the extraction method of the sophora flavescens ait is screened, and the optimal extraction method is preferably selected, so that the obtained sophora flavescens ait can obviously improve the content of various beneficial components in cordyceps sinensis mycelia. Comparing example 2 with examples 3-5, it was found that the use of amylase and cellulase had a better synergistic effect. This is probably because the sophora flavescens contains more starch, which hinders the dissolution of the active ingredients, and the enzymolysis of the starch reduces the enzymolysis hindering degree of the cellulose, and improves the enzymolysis efficiency.
Claims (5)
1. A culture medium for improving active ingredients of cordyceps sinensis mycelia is characterized by being prepared by mixing the following raw materials in parts by weight: 4-8 parts of potato juice, 2-6 parts of nutrition powder, 1-3 parts of glucose, 0.1-0.5 part of peptone, 0.02-0.08 part of monopotassium phosphate, 0.02-0.06 part of magnesium sulfate, 0.5-1 part of radix sophorae flavescentis extract and 180 parts of medical stone water; the nutritional powder is Hericium erinaceus extract and/or egg albumen powder.
2. The culture medium for improving active ingredients of cordyceps sinensis mycelia according to claim 1, wherein the potato juice is prepared by the following method, wherein the parts are all parts by weight: peeling potatoes, removing bud eyes, crushing by a crusher to obtain potato residues, mixing 8-12 parts of potato residues with 30-50 parts of water, preserving the heat at 90-100 ℃ for 40-60 minutes, and filtering by using 200-mesh and 300-mesh filter cloth to obtain potato juice.
3. The medium for increasing active ingredients of cordyceps sinensis mycelia according to claim 1, wherein: the hericium erinaceus extract is prepared by the following method, wherein the parts are all by weight: drying hericium erinaceus at 40-60 ℃ to constant weight, crushing, sieving with a 60-100-mesh sieve to obtain hericium erinaceus powder, mixing 5-10 parts of hericium erinaceus powder with 150 parts of 100-containing water, stirring for 5-10 minutes at 300 revolutions per minute of 100-containing water, then adding 0.02-0.06 part of acid protease and 0.05-0.1 part of cellulase, adjusting the pH to 3.5-4.5 by 10% hydrochloric acid by mass fraction, stirring for 1-3 hours at 300 revolutions per minute of 100-containing water at 35-45 ℃ to obtain enzymatic hydrolysate, preserving the enzymatic hydrolysate at 90-100 ℃ for 5-10 minutes, then performing vacuum concentration on the supernatant to 1.08-1.12g/mL at 40 ℃ for 10-20 minutes at 5000 revolutions per minute of 3000-containing water, and performing spray drying to obtain the hericium erinaceus extract.
4. The culture medium for increasing active ingredients of cordyceps sinensis mycelia according to claim 1, wherein the medical stone water is prepared by the following method, wherein the parts are all parts by weight: mixing 1 part of Maifanitum with 20-40 parts of water, standing at 20-30 deg.C for 20-30 min, performing ultrasonic treatment for 10-20 min, and filtering with 800 mesh filter cloth to obtain filtrate as Maifanitum water; the ultrasonic treatment conditions are as follows: the ultrasonic temperature is 40-60 ℃, the ultrasonic frequency is 25-35KHz, and the ultrasonic power is 100-.
5. The culture medium for improving active ingredients of cordyceps sinensis mycelia according to claim 1, wherein the preparation method of the sophora flavescens extracting solution comprises the following steps of:
(1) crushing radix sophorae flavescentis, sieving with a 20-50-mesh sieve to obtain radix sophorae flavescentis powder, mixing 5-10 parts of radix sophorae flavescentis powder with 60-100 parts of water, adding 0.01-0.1 part of mixed enzyme, adjusting the pH to 5.5-6.5 by using a citric acid aqueous solution with the mass fraction of 10%, and stirring at 40-50 ℃ for 1-3 hours at 300 revolutions per minute to obtain an enzymatic hydrolysate; the mixed enzyme is a mixture of 70-80 wt% of cellulase and 20-30 wt% of alpha-amylase;
(2) extracting the enzymolysis solution at 85-95 deg.C under reflux for 60-90 min, filtering with 300 mesh filter cloth to obtain filtrate and residue, adding 40-80 parts of water into the residue, extracting at 85-95 deg.C under reflux for 40-60 min, filtering with 300 mesh filter cloth, mixing the two filtrates, and concentrating under reduced pressure to density of 1.08-1.12g/mL at 50 deg.C to obtain radix Sophorae Flavescentis extract.
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Citations (3)
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| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
| CN101177665A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | Industrial method for culturing north winter worm summer herb with high-content of cordycepin |
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| WO2000030475A1 (en) * | 1998-11-26 | 2000-06-02 | Wakamoto Pharmaceutical Co., Ltd. | Hair-nourishing foods |
| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
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