CN106146684A - The isolation and purification method of ganoderma spove powder GL-B - Google Patents
The isolation and purification method of ganoderma spove powder GL-B Download PDFInfo
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- CN106146684A CN106146684A CN201610591274.1A CN201610591274A CN106146684A CN 106146684 A CN106146684 A CN 106146684A CN 201610591274 A CN201610591274 A CN 201610591274A CN 106146684 A CN106146684 A CN 106146684A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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Abstract
The invention provides the isolation and purification method of a kind of ganoderma spove powder GL-B; comprise the steps: 1) preparation of ganoderma spove powder: by conidia powder through 50 DEG C; 10h vacuum drier is dried and selects pre-treatment with 180 mesh sieves; take 1kg conidia powder and put in lucidium spore powder wall breaking machine; equipment is started in capping, and the broken time is 19min, and shutting down for 4 times during this period uncaps carries out gluing striking off of wall conidia powder; after broken wall terminates, sampling and measuring sporoderm-broken rate；2) preparation of GL-B: ganoderma spove powder is added distilled water, ultrasonic extraction, Sevag method removing protein, centrifugal, supernatant 80% ethanol, alcohol precipitation 12 h, the distilled water redissolution of gained precipitation under the conditions of 4 DEG C, obtain GL-B solution.The technique of the extraction GL-B of the present invention is simple, GL-B yield is high, pollution-free, treating capacity big, has preferable industrial applications prospect.
Description
Technical field
The present invention relates to the isolation and purification method of a kind of ganoderma spove powder GL-B, belong to biological technical field.
Background technology
Reishi sporule has the outer wall that one layer of extremely difficult chitin being digested by human body hydrochloric acid in gastric juice is constituted, the not conidia powder human body of broken wall
Cannot digest and assimilate, only open this layer of outer wall, the active ingredient being wrapped tightly by outer wall could farthest be immediately used by the body suction
Receive.After broken wall, the absorptivity to lucidum spore powder for the human body can improve 45 times more than.Spore powder with crushed sporoderm has than not spore powder with crushed sporoderm
Higher external malicious tumoricidal activity.
GL-B is the secondary metabolite of Polyporaceae Ganoderma fungal mycelium, is present in the bacterium of Ganoderma fungi
In filament and fructification.Ganoderma lucidum fruitbody is pulverized and is added the fermentation of a small amount of culture medium, and after fermentation, raw material centrifuges, supernatant cryogenic vacuum
Concentration, dialysis, ethanol precipitates, and acetone washs, removing protein, is spray-dried, and is eventually adding 10% water promotion powder and mixes.Glossy ganoderma
Polysaccharide be light brown to chocolate brown powder, dissolve in hot water.
GL-B has many pharmacologically actives: can improve immunity of organisms, accelerates blood microcirculation, improves blood and supplies
Oxygen ability, reduces the invalid oxygen demand under body inactive state, eliminates interior free yl, improves the closed stratum of body cell film,
Anti-radiation, improves liver, marrow, blood synthetic DNA, RNA, the ability of protein, extends the life-span etc..Multiple pharmacology of glossy ganoderma
Activity is mostly relevant with GL-B.
GL-B is primarily present in the cell membrane internal layer of conidia powder, causes GL-B to be difficult to extract processing.Therefore, break
Wall is the primary premise that conidia powder functional component effectively utilizes.
Content of the invention
In place of it is an object of the invention to overcome the deficiencies in the prior art, provide a kind of ganoderma spove powder GL-B
Isolation and purification method.
The isolation and purification method of the ganoderma spove powder GL-B of the present invention, comprises the steps:
1) preparation of ganoderma spove powder: by conidia powder through 50 DEG C, 10h vacuum drier is dried before selecting with 180 mesh sieves
Processing, taking 1kg conidia powder and put in lucidium spore powder wall breaking machine, equipment is started in capping, and the broken time is 19min, during this period
Shutting down for 4 times uncaps carries out striking off of viscous wall conidia powder, after broken wall terminates, sampling and measuring sporoderm-broken rate；
2) preparation of GL-B: ganoderma spove powder is added distilled water, ultrasonic extraction, Sevag method removing protein, centrifuges,
Supernatant 80% ethanol, alcohol precipitation 12 h under the conditions of 4 DEG C, gained precipitation distilled water redissolves, and obtains GL-B solution.
Preferably,
Described step 2) in, described ultrasound condition is Extracting temperature 70 DEG C, extraction time 34 min.
Described step 2) in, described centrifugal condition is 4800 r/min, 20 min.
The technique of the extraction GL-B of the present invention is simple, GL-B yield is high, pollution-free, treating capacity big, has preferably
Industrial applications prospect.
Detailed description of the invention
The isolation and purification method of the ganoderma spove powder GL-B of the present invention, comprises the steps:
1) preparation of ganoderma spove powder: by conidia powder through 50 DEG C, 10h vacuum drier is dried before selecting with 180 mesh sieves
Processing, taking 1kg conidia powder and put in lucidium spore powder wall breaking machine, equipment is started in capping, and the broken time is 19min, during this period
Shutting down for 4 times uncaps carries out striking off of viscous wall conidia powder, after broken wall terminates, sampling and measuring sporoderm-broken rate；
2) preparation of GL-B: ganoderma spove powder is added distilled water, ultrasonic extraction, Sevag method removing protein, centrifuges,
Supernatant 80% ethanol, alcohol precipitation 12 h under the conditions of 4 DEG C, gained precipitation distilled water redissolves, and obtains GL-B solution.
Described step 2) in, described ultrasound condition is Extracting temperature 70 DEG C, extraction time 34 min.
Described step 2) in, described centrifugal condition is 4800 r/min, 20 min.
Claims (3)
1. the isolation and purification method of ganoderma spove powder GL-B, it is characterised in that comprise the steps:
1) preparation of ganoderma spove powder: by conidia powder through 50 DEG C, 10h vacuum drier is dried before selecting with 180 mesh sieves
Processing, taking 1kg conidia powder and put in lucidium spore powder wall breaking machine, equipment is started in capping, and the broken time is 19min, during this period
Shutting down for 4 times uncaps carries out striking off of viscous wall conidia powder, after broken wall terminates, sampling and measuring sporoderm-broken rate；
2) preparation of GL-B: ganoderma spove powder is added distilled water, ultrasonic extraction, Sevag method removing protein, centrifuges,
Supernatant 80% ethanol, alcohol precipitation 12 h under the conditions of 4 DEG C, gained precipitation distilled water redissolves, and obtains GL-B solution.
2. the isolation and purification method of ganoderma spove powder GL-B according to claim 1, it is characterised in that described
Step 2) in, ultrasound condition is Extracting temperature 70 DEG C, extraction time 34 min.
3. the isolation and purification method of ganoderma spove powder GL-B according to claim 1, it is characterised in that described
Step 2) in, described centrifugal condition is 4800 r/min, 20 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610591274.1A CN106146684A (en) | 2016-07-26 | 2016-07-26 | The isolation and purification method of ganoderma spove powder GL-B |
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| CN201610591274.1A CN106146684A (en) | 2016-07-26 | 2016-07-26 | The isolation and purification method of ganoderma spove powder GL-B |
Publications (1)
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| CN106146684A true CN106146684A (en) | 2016-11-23 |
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|---|---|---|---|
| CN201610591274.1A Pending CN106146684A (en) | 2016-07-26 | 2016-07-26 | The isolation and purification method of ganoderma spove powder GL-B |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423284A (en) * | 2019-08-14 | 2019-11-08 | 浙江万寿康药业有限公司 | Lucid ganoderma spore powder polysaccharide has effects that auxiliary inhibits the application in the functional food of colon cancer in preparation |
| CN111041871A (en) * | 2019-12-31 | 2020-04-21 | 江苏鑫缘丝绸科技有限公司 | Dyeing method of silk floss piece based on aspergillus niger spore powder |
| CN111074646A (en) * | 2019-12-31 | 2020-04-28 | 江苏鑫缘丝绸科技有限公司 | Silk floss piece dyeing method based on monascus spore powder |
| CN111116773A (en) * | 2020-03-02 | 2020-05-08 | 浙江养芝康生物科技有限公司 | Ganoderma lucidum β glucan GLBG component and preparation process and application thereof |
| WO2020093510A1 (en) * | 2018-11-06 | 2020-05-14 | 中科健康产业集团江苏药业有限公司 | Separation and purification method for polysaccharide in ganoderma lucidum spores |
| CN111286997A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on phellinus igniarius spore powder |
| CN111286998A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on ganoderma lucidum spore powder |
| CN113278535A (en) * | 2021-06-30 | 2021-08-20 | 四川省中医药科学院 | New ganoderma lucidum strain ZL167 and new application thereof |
| CN114874349A (en) * | 2022-06-15 | 2022-08-09 | 河北大学 | Method for separating and purifying ganoderan based on field flow separation technology |
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| CN102008515A (en) * | 2010-11-26 | 2011-04-13 | 江南大学 | Construction method of ganoderma spore powder polysaccharide fingerprint and standard fingerprint of ganoderma spore powder polysaccharide |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020093510A1 (en) * | 2018-11-06 | 2020-05-14 | 中科健康产业集团江苏药业有限公司 | Separation and purification method for polysaccharide in ganoderma lucidum spores |
| CN110423284A (en) * | 2019-08-14 | 2019-11-08 | 浙江万寿康药业有限公司 | Lucid ganoderma spore powder polysaccharide has effects that auxiliary inhibits the application in the functional food of colon cancer in preparation |
| CN111041871B (en) * | 2019-12-31 | 2022-06-10 | 江苏鑫缘丝绸科技有限公司 | Dyeing method of silk floss piece based on aspergillus niger spore powder |
| CN111041871A (en) * | 2019-12-31 | 2020-04-21 | 江苏鑫缘丝绸科技有限公司 | Dyeing method of silk floss piece based on aspergillus niger spore powder |
| CN111074646A (en) * | 2019-12-31 | 2020-04-28 | 江苏鑫缘丝绸科技有限公司 | Silk floss piece dyeing method based on monascus spore powder |
| CN111074646B (en) * | 2019-12-31 | 2022-06-10 | 江苏鑫缘丝绸科技有限公司 | Silk floss piece dyeing method based on monascus spore powder |
| CN111116773A (en) * | 2020-03-02 | 2020-05-08 | 浙江养芝康生物科技有限公司 | Ganoderma lucidum β glucan GLBG component and preparation process and application thereof |
| CN111286998A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on ganoderma lucidum spore powder |
| CN111286997B (en) * | 2020-03-23 | 2022-06-10 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on phellinus igniarius spore powder |
| CN111286997A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on phellinus igniarius spore powder |
| CN113278535A (en) * | 2021-06-30 | 2021-08-20 | 四川省中医药科学院 | New ganoderma lucidum strain ZL167 and new application thereof |
| CN114874349A (en) * | 2022-06-15 | 2022-08-09 | 河北大学 | Method for separating and purifying ganoderan based on field flow separation technology |
| CN114874349B (en) * | 2022-06-15 | 2023-03-10 | 河北大学 | Method for separating and purifying ganoderan based on field flow separation technology |
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