CN105131146A - Combined extraction of beta-glucan and beta-mannan in yeast cell walls - Google Patents
Combined extraction of beta-glucan and beta-mannan in yeast cell walls Download PDFInfo
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- CN105131146A CN105131146A CN201510515576.6A CN201510515576A CN105131146A CN 105131146 A CN105131146 A CN 105131146A CN 201510515576 A CN201510515576 A CN 201510515576A CN 105131146 A CN105131146 A CN 105131146A
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- mannosans
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- degreasing
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Abstract
Beta-(1,3)-D-glucan and mannan in yeast cell walls have the biological functions of activating immune cells, regulating the body immunity, having antibacterial, anti-inflammatory, antivirus and antitumor functions and the like, and have wide application prospects. The invention relates to a method for extracting beta-(1,3)-D-glucan and mannan from the saccharomyces cerevisiae cell walls. The yeast cell walls are subjected to six steps of wall breaking, high temperature extraction, centrifugal separation, alcohol precipitation, drying, degreasing and enzymolysis to prepare beta-(1,3)-D-glucan and beta-mannan.
Description
Technical field
The present invention relates to the method extracting β-(1,3)-D-dextran and beta-mannase from yeast cells wall.It is characterized in that, for taking yeast cells wall as raw material, preparing β-(1,3)-D-dextran and beta-mannase by broken wall, high temperature extraction, centrifugation, alcohol precipitation, drying, degreasing, enzymolysis six steps.
Background technology
Yeast saccharomyces cerevisiae polysaccharide is one of important sources of polysaccharide, β wherein-(1,3) the multiple life that the cell polysaccharide such as-D-dextran, mannosans participates in cell regulates, biological functions such as having immune cell activated, regulate immunity of organisms, tool antisepsis and anti-inflammation, be antiviral, antitumor.Yeast saccharomyces cerevisiae polysaccharide can extract from brewing fermentation liquid, but is more obtain from yeast cells wall.Yeast cells wall accounts for 30% of dry cell weight, and the beta-glucan by 35% ~ 45%, the mannosans of 40% ~ 45%, the protein of 5% ~ 10%, the lipid of 3% ~ 8%% and 1% ~ 2% chitin form, 1% ~ 3% inorganic salt composition.Cell walls from inside to outside divides 3 layers, is respectively dextran, protein, mannosans.
β-(1,3)-D-dextran has result for the treatment of to illnesss such as tumour, hepatitis, diabetes, cardiovascular, hyperlipidemia; Be a kind of good biological effect conditioning agent, it improves body immunity by stimulating immune system, interdepends in airframe systems people, very crucial to maintenance homeostasis.Yeast mannans has immunity, and the effect such as antitumor.Because human body self cannot synthesize β-(1,3)-D-dextran and mannosans, by they successful separation and Extraction, significant for relevant disease treatment.
At present, more to the extracting method of yeast cell wall beta-(1,3)-D-dextran and mannosans both at home and abroad, mainly contain acidity extraction, alkalinity extraction, soda acid one method, organic solvent extraction, ultrasonic extraction, enzyme process etc.Various method cuts both ways, and relative merits are obvious.
The states such as Japan, the U.S. further investigate in a large number to β-(1,3)-D-dextran and mannosans, will extract β-(1,3)-dextran and be widely used in the fields such as medicine, food, aquaculture, makeup.China is wine brewing big country, and yeast cells wall source is sufficient.The yeast slurry being rich in yeast cells wall is mostly processed as cheap roughage or is directly discharged, and causes very large resource waste and environmental pollution.Therefore, seek and optimize a kind of highly effective extraction method, for improving β-(1,3)-D-dextran, mannosans yield and quality further, the minimizing wasting of resources and environmental pollution have extremely important meaning.
For the market requirement of β-(1,3)-D-dextran, mannosans, the invention provides a kind of is raw material with yeast cells wall, extracts the method for β-(1,3)-D-dextran and mannosans.
Summary of the invention
The present invention relates to yeast cells wall is raw material, extracts the method for β-(1,3)-D-dextran and mannosans.The present invention includes: (1) broken wall (2) high temperature extraction (3) centrifugation (4) alcohol precipitation, drying (5) degreasing (6) enzymolysis six steps.
(1) broken wall
Yeast cells wall dispersion is sized mixing into the suspension liquid of cell concentration about 10%, after colloidal mill grinding, add clarifixator and carry out broken wall treatment.
(2) high temperature extraction
By the yeast cells wall after broken wall treatment at 120 DEG C of extracting 3 ~ 4h.
(3) centrifugation
Put into whizzer, centrifugation after the homogeneous liquid cooling obtained by high temperature extraction, obtain supernatant liquor and precipitation.
(4) concentrated, alcohol precipitation
By the centrifugal supernatant liquor suction filtration obtained, removing suspended impurity, after vacuum concentration, adds ethanol and carries out substep alcohol precipitation.Filtration, vacuum-drying obtain mannosans.The centrifugal lower sediment obtained obtains beta-glucan by lyophilize.
(5) degreasing
The mannosans obtain (4) and beta-glucan are made into suspension from different organic solvents, reflux respectively, carry out skimming treatment, obtain degreasing mannosans and degreasing beta-glucan.
(6) enzymolysis
The degreasing mannosans obtained and degreasing beta-glucan are mixed with protein enzyme solution respectively, controls temperature of reaction and carry out enzymolysis processing.After reaction terminates, the residue obtained washes with water, centrifugal, then adds water and redissolve post-heating and to go out enzyme, and centrifugal, vacuum-drying, obtains mannosans and beta-glucan product.
Claims (9)
1. one kind is extracted the method for β-(1,3)-D-dextran and mannosans from yeast cells wall.β-(1,3)-D-dextran and mannosans have immune cell activated, regulate immunity of organisms, tool antisepsis and anti-inflammation, the biological function such as antiviral, antitumor.
2. the extracting method of β according to claim 1-(1,3)-D-dextran and mannosans comprises broken wall, high temperature extraction, centrifugation, alcohol precipitation, drying, degreasing, enzymolysis six steps.
3. wall breaking technology according to claim 2, is characterized in that yeast cells wall to make suspension liquid, after colloidal mill grinding, adds clarifixator and processes.
4. the high temperature extraction in claim 2, refers in a kettle., is controlled by temperature of reaction system at 115 ~ 125 DEG C, extracting 3 ~ 4h.
5. step with centrifugal separation according to claim 2 carries out centrifugation with whizzer after being characterised in that the homogeneous liquid cooling obtained by high temperature extraction, obtains supernatant liquor and precipitation.
6. the feature of alcohol precipitation according to claim 2 is the centrifugal supernatant liquor suction filtration obtained, and removing suspended impurity, after vacuum concentration, adds ethanol and carry out substep alcohol precipitation, filtration.
7. drying according to claim 2 is characterised in that the solid (5 solids obtained are callose crude products, and 6 solids obtained are mannosans crude products) by (5) and (6) obtain processes with lyophilize and vacuum-drying respectively.
8. degreasing according to claim 2 is characterised in that the mannosans that (7) obtained and beta-glucan are made into suspension from different organic solvents, reflux respectively, carries out skimming treatment, obtains degreasing mannosans and degreasing beta-glucan.
9. enzymolysis according to claim 2 is characterised in that and the degreasing mannosans obtained and degreasing beta-glucan is mixed with protein enzyme solution respectively, controls temperature of reaction and carries out enzymolysis, obtain mannosans and beta-glucan product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510515576.6A CN105131146A (en) | 2015-08-18 | 2015-08-18 | Combined extraction of beta-glucan and beta-mannan in yeast cell walls |
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| CN201510515576.6A CN105131146A (en) | 2015-08-18 | 2015-08-18 | Combined extraction of beta-glucan and beta-mannan in yeast cell walls |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505903A (en) * | 2016-01-05 | 2016-04-20 | 中国科学院天津工业生物技术研究所 | Method for preparing induction sugar and inducing the synthesis of cellulose through yeast cells |
| CN108524925A (en) * | 2018-04-03 | 2018-09-14 | 潍坊华英生物科技有限公司 | A kind of inactive yeast bacterium injection |
| CN109645109A (en) * | 2019-01-07 | 2019-04-19 | 浙江大学 | The fruit disease control method of resistant activity is lured based on brewing yeast cell wall product |
| WO2020034948A1 (en) * | 2018-08-13 | 2020-02-20 | Lifenergy Biotech Corp. | Method for in vitro activation of immune cells |
| CN112760346A (en) * | 2020-12-29 | 2021-05-07 | 唐山拓普生物科技有限公司 | Method for extracting yeast cell wall polysaccharide with high immunocompetence |
| CN114081107A (en) * | 2021-12-16 | 2022-02-25 | 河北省农林科学院滨海农业研究所 | Feed additive for improving immunity and egg quality of laying hens and preparation method thereof |
| CN116370360A (en) * | 2023-02-09 | 2023-07-04 | 北京活力蓝晶生物科技有限公司 | Production process for producing yeast cell wall extract hydrolysate by using organic acid |
| CN117467648A (en) * | 2023-12-27 | 2024-01-30 | 潍坊新希望六和饲料科技有限公司 | Application of yeast zymolyte in preparation of feed additive |
-
2015
- 2015-08-18 CN CN201510515576.6A patent/CN105131146A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505903A (en) * | 2016-01-05 | 2016-04-20 | 中国科学院天津工业生物技术研究所 | Method for preparing induction sugar and inducing the synthesis of cellulose through yeast cells |
| CN105505903B (en) * | 2016-01-05 | 2019-03-22 | 中国科学院天津工业生物技术研究所 | Induction sugar and cellulase induction synthetic method are prepared using yeast cells |
| CN108524925A (en) * | 2018-04-03 | 2018-09-14 | 潍坊华英生物科技有限公司 | A kind of inactive yeast bacterium injection |
| CN108524925B (en) * | 2018-04-03 | 2020-12-01 | 潍坊华英生物科技有限公司 | Inactivated yeast injection |
| WO2020034948A1 (en) * | 2018-08-13 | 2020-02-20 | Lifenergy Biotech Corp. | Method for in vitro activation of immune cells |
| CN109645109A (en) * | 2019-01-07 | 2019-04-19 | 浙江大学 | The fruit disease control method of resistant activity is lured based on brewing yeast cell wall product |
| CN112760346A (en) * | 2020-12-29 | 2021-05-07 | 唐山拓普生物科技有限公司 | Method for extracting yeast cell wall polysaccharide with high immunocompetence |
| CN112760346B (en) * | 2020-12-29 | 2022-09-09 | 唐山拓普生物科技有限公司 | Method for extracting yeast cell wall polysaccharide with high immunocompetence |
| CN114081107A (en) * | 2021-12-16 | 2022-02-25 | 河北省农林科学院滨海农业研究所 | Feed additive for improving immunity and egg quality of laying hens and preparation method thereof |
| CN114081107B (en) * | 2021-12-16 | 2023-08-04 | 河北省农林科学院滨海农业研究所 | Feed additive for improving immunity and egg quality of laying hens and preparation method thereof |
| CN116370360A (en) * | 2023-02-09 | 2023-07-04 | 北京活力蓝晶生物科技有限公司 | Production process for producing yeast cell wall extract hydrolysate by using organic acid |
| CN116370360B (en) * | 2023-02-09 | 2023-11-14 | 北京活力蓝晶生物科技有限公司 | Production process for producing yeast cell wall extract hydrolysate by using organic acid |
| CN117467648A (en) * | 2023-12-27 | 2024-01-30 | 潍坊新希望六和饲料科技有限公司 | Application of yeast zymolyte in preparation of feed additive |
| CN117467648B (en) * | 2023-12-27 | 2024-04-23 | 潍坊新希望六和饲料科技有限公司 | Application of yeast zymolyte in preparation of feed additive |
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Addressee: Hu Xiangzheng Document name: Notification of before Expiration of Request of Examination as to Substance |
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Application publication date: 20151209 |