CN103789092B

CN103789092B - Essential oil separating and purifying method of oat bran

Info

Publication number: CN103789092B
Application number: CN201410014764.6A
Authority: CN
Inventors: 王玉琢; 逯增晟; 段云
Original assignee: Zhangjiakou Yikang Biotechnology Co ltd
Current assignee: Zhangjiakou Yikang Biotechnology Co ltd
Priority date: 2014-01-14
Filing date: 2014-01-14
Publication date: 2017-01-18
Anticipated expiration: 2034-01-14
Also published as: CN103789092A

Abstract

The invention relates to an essential oil separating and purifying method of oat bran. The method comprises the following steps: finely grinding and screening the oat bran; carrying out enzymolysis by medium temperature amylase; carrying out enzymolysis by alpha-amylase; carrying out enzymolysis by protease and a glucose saccharifying enzyme; separating by a tank horizontal spiral centrifugal machine at 3800-4000r/min, and separating the essential oil from centrifugal clean liquid through an oil-water separator at a rotary speed of 14000-15000r/min; putting water-containing essential oil into ceramic film microfiltration equipment to remove microorganisms and macromolecular impurities in the essential oil; inputting the purified essential oil into ceramic film ultrafiltration concentration equipment, concentrating and separating other polysaccharides and monosaccharides as well as other micromolecular impurities in the essential oil, and finally concentrating to remove moisture. According to the invention, the extraction process is replaced by matching ceramic film separating technology with centrifugal equipment and the like in use, and the essential oil is extracted under the premise of not using any chemical agents, so that the natural product is extracted by a physical method.

Description

Essential oil isolation and purification method in a kind of oat bran

Technical field

The present invention relates to essential oil isolation and purification method in a kind of oat bran.

Background technology

Oat, also known as bromegrass, wild wheat, are the important Cereal grain of the mankind, there are plantation in many countries in the world.In river Northern Zhangjiakou region governmental input substantial contribution develops organic oat industry, and at present, Zhangjiakou region amount of oat yield occupies China the One.Meanwhile, Zhangjiakou region oat secondary industry compares concentration, such as oatmeal, and the product such as oatmeal has found a good sale in domestic various places.

Oat bran is outcast product in oat process, and people ignored the nutritive value that it is rich in the past, only Using oat bran as culturing feed, significantly waste its nutritive value.It is up to 9.8% in beta-glucosan from oat bran content, Oat egg white matter content is up to 10.2%, and oat oil content is up to 9.4%.Beta glucan, have regulation blood fat, metabolism of blood glucose and The effect of multiple diseases such as prevention intestinal cancer.Oat egg white matter has 8 kinds of amino acid necessary to the mankind, its each amino acid ratio ratio More reasonable.Oat essential oil is the cosmetic material of high-quality, has the effects such as moisturizing, anti aging effect, anti-skin oxidative.

Relatively more early to oat extract consciousness abroad, it is concentrated mainly on American-European countries, and produced in batches.And it is domestic Oat extraction industry development ratio is later, only develops part oat extract at present, major part is also in laboratory development. Domestic existing producer develops beta glucan extraction process in oat bran, and has commercially produced in batches and sold, and swallow Malt extract oil, oat egg white matter product Yin Qiben saves price, extractive technique, and extraction yield etc. limits rare producer input amount at home Produce.

Existing oat extract industry someone achieves the method extracting oat nutrient content respectively at home.But swallow The project that wheat extract industry realizes large-scale production is few.Guangzhou at home, Xi'an, Jiangsu, there is oat beta glucan on the ground such as Hebei Extraction company.But oat extract industrialized production is mostly in development, realizes large-scale production few.A lot of techniques Still it is in ethanol precipitation, ammonium sulfate precipitation etc. extracts means.This technique can bring secondary pollution to product, does not ensure its safety Use.

Content of the invention

The purpose of the present invention is to be used with coordinative composition of equipments such as centrifugations using Ceramic Membranes Separating Technique, replaces extraction process, Do not use on the premise of any chemical agent by extraction of essential oil out, realize natural products Physical and extract.

Technical scheme is as follows:

Essential oil isolation and purification method in a kind of oat bran is it is characterised in that methods described step is as follows:

(1) oat bran fine gtinding screening, grain fineness 80-100 mesh；

(2) oat bran and water quality proportioning 1:6, puts into reactor and is warming up to 50-60 DEG C, add by volume

1.2% middle temperature amylase, digests 1.5-2 hour；

(3) feed liquid is warming up to 90-102 DEG C, adds 0.5% AMS by volume, digest 45-55min；

(4) feed liquid is cooled to 55-65 DEG C, adds 0.2% protease and 0.5% glucase by volume, digests 45- 55min；

(5) put tank horizontal screw centrifuge to separate, 3800-4000 r/min, remove wheat bran and other bulky grain dregs, retain from Heart clear liquid；

(6) centrifugal clear liquid, through oil-water separator, rotating speed 14000-15000r/min, essential oil is separated；

(7) aqueous essential oil is put into microfiltration of ceramic membrane equipment, remove microorganism and big molecular impurity in essential oil；

(8) purify essential oil and put into ceramic membrane ultrafitration concentrator, concentrating and separating fall in essential oil other polysaccharide monose and other Small molecular weight impurity, finally concentrates and removes moisture removal.

The technique of the present invention is suitable for enterprise's volume production, puts into less, and product quality is high, and this technique easily realizes the different rule of technique Mould produces.Meanwhile, the present invention is easily controlled microorganism, and realizes no protective system interpolation.Meet American-European safety requirements, product makes Wide with scope.Compared with prior art:

1st, not using chemical agent, purely mechanic equipment extracts the present invention.

2nd, equipment operation is simple, and extraction time is short, easy industrialized production.

3rd, the method for the present invention no protective system.

Specific embodiment

Embodiment 1:

(1) oat bran fine gtinding screening, grain fineness 80 mesh；

(2) oat bran and water quality proportioning 1:6, puts into reactor and is warming up to 50 DEG C, adds 1.2% middle temperature to form sediment by volume Powder enzyme, digests 1.5 hours；

(3) feed liquid is warming up to 90 DEG C, adds 0.5% AMS by volume, digest 45min；

(4) feed liquid is cooled to 55 DEG C, adds 0.2% protease and 0.5% glucase by volume, digests 45min；

(5) put tank horizontal screw centrifuge to separate, 3800r/min, remove wheat bran and other bulky grain dregs, retain centrifugation clear Liquid；

(6) centrifugal clear liquid, through oil-water separator, rotating speed 14000r/min, essential oil is separated；

Embodiment 2:

(1) oat bran fine gtinding screening, grain fineness 90 mesh；

(2) oat bran and water quality proportioning 1:6, puts into reactor and is warming up to 55 DEG C, add by volume

1.2% middle temperature amylase, digests 2 hours；

(3) feed liquid is warming up to 95 DEG C, adds 0.5% AMS by volume, digest 50min；

(4) feed liquid is cooled to 60 DEG C, adds 0.2% protease and 0.5% glucase by volume, digests 50min；

(5) put tank horizontal screw centrifuge to separate, 4000 r/min, remove wheat bran and other bulky grain dregs, retain centrifugation clear Liquid；

(6) centrifugal clear liquid, through oil-water separator, rotating speed 14500r/min, essential oil is separated；

Embodiment 3:

(1) oat bran fine gtinding screening, grain fineness 100 mesh；

(2) oat bran and water quality proportioning 1:6, puts into reactor and is warming up to 60 DEG C, adds 1.2% middle temperature to form sediment by volume Powder enzyme, digests 2 hours；

(5) put tank horizontal screw centrifuge to separate, 3900 r/min, remove wheat bran and other bulky grain dregs, retain centrifugation clear Liquid；

(6) centrifugal clear liquid, through oil-water separator, rotating speed 15000r/min, essential oil is separated；

Claims

1. in a kind of oat bran essential oil isolation and purification method it is characterised in that methods described step is as follows:

(1) oat bran fine gtinding screening, grain fineness 80-100 mesh；

(2) oat bran and water quality proportioning 1:6, puts into reactor and is warming up to 50-60 DEG C, adds 1.2% middle temperature to form sediment by volume Powder enzyme, digests 1.5-2 hour；

(5) put into horizontal screw centrifuge to separate, 3800-4000r/min, remove wheat bran and other bulky grain dregs, retain centrifugation clear Liquid；

(8) purify essential oil and put into ceramic membrane ultrafitration concentrator, concentrating and separating falls in essential oil other polysaccharide monose and other little point Sub- impurity, finally concentrates and removes moisture removal.