CN103789093A - Method for extracting plural nutrient materials from oat bran by one-step process - Google Patents
Method for extracting plural nutrient materials from oat bran by one-step process Download PDFInfo
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- CN103789093A CN103789093A CN201410014765.0A CN201410014765A CN103789093A CN 103789093 A CN103789093 A CN 103789093A CN 201410014765 A CN201410014765 A CN 201410014765A CN 103789093 A CN103789093 A CN 103789093A
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Abstract
The invention relates to a method for extracting a plurality of nutrient materials from oat bran by the one-step process. The method mainly comprises the following steps of: finely grinding and sieving the oat bran; performing enzymolysis with medium temperature amylase for 1.5 to 2 hours; performing enzymolysis with alpha-amylase; carrying out enzymolysis with 0.3% protease and 0.6% glucose saccharifying enzyme; separating through a decanter centrifuge at speed of 3500 to 4000 rotations per minute to remove bran and other large particular dregs and remaining the centrifugal supernatant; processing the centrifugal supernatant by an oil-water separator at a rotating speed of 14000 to 15000 rotations per minute to separate out the essential oil; processing the separated-out rough essential oil by ceramic membrane microfiltration equipment so as to remove macromolecular impurities; separating and purifying through ceramic membrane ultrafiltration equipment to remove micromolecular impurities; then concentrating to obtain the essential oil. The method has the beneficial effects that the oat bran resource is reasonably utilized, no chemical agents are used, the extraction is completely performed by mechanical equipment, the equipment operation is simple, cross operation of equipment enables the decrease of equipment investment, and the extraction costs a little time; in addition, the obtained product is high yield and purity and easy for industrial production.
Description
Technical field
The present invention relates to a kind of single stage method and extract the method for multiple nutritional components material in oat bran.
Background technology
Oat, has another name called bromegrass, wild wheat, is the important Cereal grains of the mankind, and there is plantation in many countries in the world.Drop into substantial contribution in body in Zhangjiakou Area, Hebei Province district government and develop organic oat industry, at present, Zhangjiakou region amount of oat yield occupies China first.Meanwhile, Zhangjiakou region oat secondary industry is more concentrated, for example rolled oats, and the products such as oatmeal have been found a good sale in domestic various places.
Oat bran is outcast product in the oat course of processing, and former people ignore its nutritive value being rich in, and only using oat bran as culturing feed, has greatly wasted its nutritive value.At beta-glucosan from oat bran content, up to 9.8%, avenin matter content is up to 10.2%, and oat oil content is up to 9.4%.Beta-glucan, has the effect of various diseases such as regulating blood fat, metabolism of blood glucose and prevention intestinal cancer.Avenin matter has necessary 8 seed amino acids of the mankind, and its each amino acid ratio is more reasonable.Oat essential oil is the cosmetic material of high-quality, has moisturizing, anti aging effect, the effects such as anti-skin oxidation.
To oat extract consciousness relatively early, mainly concentrate on American-European countries, and produced in batches abroad.And domestic oat extraction industry development is more late, only develop at present part oat extract, major part is also in laboratory development.Domestic existing producer has developed beta glucan extraction process in oat bran, and existing batch production and sale on market, and oat essential oil, avenin matter product Yin Qiben economizes price, extractive technique, and extraction yields etc. are limited in domestic rare producer and drop into volume production.
Existing oat extract industry has people to realize the method for extracting respectively an oat nutritive ingredient at home.It is few that but oat extract industry realizes the project of scale production.Guangzhou at home, Xi'an, Jiangsu, the ground such as Hebei have oat beta glucan to extract company.But oat extract suitability for industrialized production major part, in development, realizes scale production few.A lot of technique is still in ethanol precipitation, the extraction means such as ammonium sulfate precipitation.This technique can be brought secondary pollution to product, does not ensure its safe handling.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of single stage method can extract the method for multiple nutritional components material in oat bran.
Technical scheme of the present invention is:
Single stage method is extracted a method for multiple nutritional components material in oat bran, it is characterized in that, described method steps is as follows:
(1) oat bran fine grinding screening, particle fineness 80-120 order;
(2) oat bran and quality proportioning 1:6, drops into reactor and is warming up to 55-65 ℃, adds by volume 1.2% middle temperature amylase, enzymolysis 1.5-2 hour;
(3) feed liquid is warming up to 90-100 ℃, adds by volume 0.5% α-amylase, enzymolysis 45-55min;
(4) feed liquid is cooled to 55-65 ℃, adds by volume 0.3% proteolytic enzyme and 0.6% glucase,
Enzymolysis 30-45min, puts tank and is for further processing;
(5) horizontal screw centrifuge separates, and 3500-4000r/min removes wheat bran and other macrobead dregs, retains centrifugal clear liquid;
(6) centrifugal clear liquid is through oil-water separator, and rotating speed 14000-15000r/min, separates essential oil; Isolated essential oil crude product, through microfiltration of ceramic membrane equipment, is removed macromole impurity, then passes through ceramic membrane ultrafitration device separates purifying, removes small molecular weight impurity; The finally essential oil finished product of concentrated system.
Further, described method further comprises the steps:
(7) feed liquid of step (6) being removed to grease is through microfiltration of ceramic membrane equipment, remove macromole impurity, then pass through ceramic membrane ultrafitration separation and purification, remove small molecular weight impurity, and preserve through liquid, ceramic membrane concentrated solution is beta glucan precision processing liquid, makes powder through freeze-drier, obtains oat beta glucan product.
Further, described method further comprises the steps:
(8) what step (7) is produced sees through liquid through ceramic membrane nanofiltration device separates purifying, removes small molecular weight impurity, after ceramic membrane equipment is concentrated, has both obtained oat polypeptide product.
Beneficial effect of the present invention is:
1, present method has rationally been utilized oat bran resource, does not use chemical agent.Pure mechanical means is extracted.
2, operation of equipment is simple, and can reduce facility investment by cross-reference, and extraction time is short, and product yield is high, and purity is high, easily suitability for industrialized production.
3, each product, without protective system, does not use organic medicament, the use safety of avoiding medicament to bring.Meet safe handling.
4, a step multi-product, has saved oat bran resource, saves labor force, reduces production cost.
Embodiment
The ceramic membrane using in the embodiment of the present invention, with titanium dioxide, zirconium dioxide is raw material, sintering film forming pipe plates the film of different pore size at ceramic membrane film inside pipe wall, realize separation and purification object.
Embodiment 1
(1) oat bran fine grinding screening, particle fineness 80 orders;
(2) oat bran and quality proportioning 1:6, drops into reactor and is warming up to 55 ℃, adds by volume 1.2% middle temperature amylase, enzymolysis 1.5 hours;
(3) feed liquid is warming up to 90, adds by volume 0.5% α-amylase, enzymolysis 45min;
(4) feed liquid is cooled to 55 ℃, adds by volume 0.3% proteolytic enzyme and 0.6% glucase,
Enzymolysis 30min, puts tank and is for further processing;
(5) horizontal screw centrifuge separates, and 3500r/min removes wheat bran and other macrobead dregs, retains centrifugal clear liquid;
(6) centrifugal clear liquid is through oil-water separator, and rotating speed 14000r/min, separates essential oil; Isolated essential oil crude product, through microfiltration of ceramic membrane equipment, is removed macromole impurity, then passes through ceramic membrane ultrafitration device separates purifying, removes small molecular weight impurity; The finally essential oil finished product of concentrated system.
Embodiment 2
By the feed liquid of removing grease in embodiment 1 through microfiltration of ceramic membrane equipment, remove macromole impurity, then pass through ceramic membrane ultrafitration separation and purification, remove small molecular weight impurity, and preserve through liquid, ceramic membrane concentrated solution is beta glucan precision processing liquid, makes powder through freeze-drier, obtains oat beta glucan product.
Embodiment 3
Purify through ceramic membrane nanofiltration device separates with the liquid that sees through producing in embodiment 2, remove small molecular weight impurity, after ceramic membrane equipment is concentrated, both obtained oat polypeptide product.
Embodiment 4
(1) oat bran fine grinding screening, particle fineness 100 orders;
(2) oat bran and quality proportioning 1:6, drops into reactor and is warming up to 60 ℃, adds by volume 1.2% middle temperature amylase, enzymolysis 2 hours;
(3) feed liquid is warming up to 95 ℃, adds by volume 0.5% α-amylase, enzymolysis 50min;
(4) feed liquid is cooled to 60 ℃, adds by volume 0.3% proteolytic enzyme and 0.6% glucase,
Enzymolysis 40min, puts tank and is for further processing;
(5) horizontal screw centrifuge separates, and 4000r/min removes wheat bran and other macrobead dregs, retains centrifugal clear liquid;
(6) centrifugal clear liquid is through oil-water separator, and rotating speed 14500r/min, separates essential oil; Isolated essential oil crude product, through microfiltration of ceramic membrane equipment, is removed macromole impurity, then passes through ceramic membrane ultrafitration device separates purifying, removes small molecular weight impurity; The finally essential oil finished product of concentrated system.
Embodiment 5
By the feed liquid of removing grease in embodiment 4 through microfiltration of ceramic membrane equipment, remove macromole impurity, then pass through ceramic membrane ultrafitration separation and purification, remove small molecular weight impurity, and preserve through liquid, ceramic membrane concentrated solution is beta glucan precision processing liquid, makes powder through freeze-drier, obtains oat beta glucan product.
Embodiment 6
Purify through ceramic membrane nanofiltration device separates with the liquid that sees through producing in embodiment 2, remove small molecular weight impurity, after ceramic membrane equipment is concentrated, both obtained oat polypeptide product.
Embodiment 7
(1) oat bran fine grinding screening, particle fineness 80-120 order;
(2) oat bran and quality proportioning 1:6, drops into reactor and is warming up to 65 ℃, adds by volume 1.2% middle temperature amylase, enzymolysis 2 hours;
(3) feed liquid is warming up to 100 ℃, adds by volume 0.5% α-amylase, enzymolysis 55min;
(4) feed liquid is cooled to 65 ℃, adds by volume 0.3% proteolytic enzyme and 0.6% glucase,
Enzymolysis 45min, puts tank and is for further processing;
(5) horizontal screw centrifuge separates, and 4000r/min removes wheat bran and other macrobead dregs, retains centrifugal clear liquid;
(6) centrifugal clear liquid is through oil-water separator, and rotating speed 15000r/min, separates essential oil; Isolated essential oil crude product, through microfiltration of ceramic membrane equipment, is removed macromole impurity, then passes through ceramic membrane ultrafitration device separates purifying, removes small molecular weight impurity; The finally essential oil finished product of concentrated system.
Embodiment 8
By the feed liquid of removing grease in embodiment 7 through microfiltration of ceramic membrane equipment, remove macromole impurity, then pass through ceramic membrane ultrafitration separation and purification, remove small molecular weight impurity, and preserve through liquid, ceramic membrane concentrated solution is beta glucan precision processing liquid, makes powder through freeze-drier, obtains oat beta glucan product.
Embodiment 9
Purify through ceramic membrane nanofiltration device separates with the liquid that sees through producing in embodiment 8, remove small molecular weight impurity, after ceramic membrane equipment is concentrated, both obtained oat polypeptide product.
Claims (3)
1. single stage method is extracted a method for multiple nutritional components material in oat bran, it is characterized in that, described method steps is as follows:
(1) oat bran fine grinding screening, particle fineness 80-120 order;
(2) oat bran and quality proportioning 1:6, drops into reactor and is warming up to 55-65 ℃, adds by volume 1.2% middle temperature amylase, enzymolysis 1.5-2 hour;
(3) feed liquid is warming up to 90-100 ℃, adds by volume 0.5% α-amylase, enzymolysis 45-55min;
(4) feed liquid is cooled to 55-65 ℃, adds by volume 0.3% proteolytic enzyme and 0.6% glucase,
Enzymolysis 30-45min, puts tank and is for further processing;
(5) horizontal screw centrifuge separates, and 3500-4000r/min removes wheat bran and other macrobead dregs, retains centrifugal clear liquid;
(6) centrifugal clear liquid is through oil-water separator, and rotating speed 14000-15000r/min, separates essential oil; Isolated essential oil crude product, through microfiltration of ceramic membrane equipment, is removed macromole impurity, then passes through ceramic membrane ultrafitration device separates purifying, removes small molecular weight impurity; The finally essential oil finished product of concentrated system.
2. method according to claim 1, is characterized in that, described method further comprises the steps:
(7) feed liquid of step (6) being removed to grease is through microfiltration of ceramic membrane equipment, remove macromole impurity, then pass through ceramic membrane ultrafitration separation and purification, remove small molecular weight impurity, and preserve through liquid, ceramic membrane concentrated solution is beta glucan precision processing liquid, makes powder through freeze-drier, obtains oat beta glucan product.
3. method according to claim 2, is characterized in that, described method further comprises the steps:
(8) what step (7) is produced sees through liquid through ceramic membrane nanofiltration device separates purifying, removes small molecular weight impurity, after ceramic membrane equipment is concentrated, has both obtained oat polypeptide product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410014765.0A CN103789093B (en) | 2014-01-14 | A kind of one-step method extracts the method for multiple nutritional components material in bran of Fagopyrum esculentum Moench |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410014765.0A CN103789093B (en) | 2014-01-14 | A kind of one-step method extracts the method for multiple nutritional components material in bran of Fagopyrum esculentum Moench |
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| CN103789093A true CN103789093A (en) | 2014-05-14 |
| CN103789093B CN103789093B (en) | 2016-11-30 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105440101A (en) * | 2015-12-02 | 2016-03-30 | 华南理工大学 | Method for preparing buckwheat protein concentrate rich in polyphenols |
| CN106307445A (en) * | 2016-08-18 | 2017-01-11 | 青岛花帝食品配料有限公司 | Method for preparing beef bone essence |
| CN106905142A (en) * | 2017-04-18 | 2017-06-30 | 四川龙蟒福生科技有限责任公司 | A kind of isolation and purification method of S abscisic acids |
| CN107647285A (en) * | 2017-09-25 | 2018-02-02 | 贺州学院 | A kind of enzyme solution of oat |
| CN109280683A (en) * | 2017-08-15 | 2019-01-29 | 广东丸美生物技术股份有限公司 | Preparation method of composite plant hydrolyzed protein peptide |
| CN109400751A (en) * | 2019-01-07 | 2019-03-01 | 张家口康生物科技有限公司 | A kind of low content oat beta-glucan extracting method |
| CN111187763A (en) * | 2019-11-22 | 2020-05-22 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for oat hydrolysis and application thereof |
| CN112521525A (en) * | 2020-11-30 | 2021-03-19 | 福州美乐莲生物科技有限公司 | Production process for separating and purifying beta-glucan from oat bran |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105440101A (en) * | 2015-12-02 | 2016-03-30 | 华南理工大学 | Method for preparing buckwheat protein concentrate rich in polyphenols |
| CN105440101B (en) * | 2015-12-02 | 2019-01-29 | 华南理工大学 | A kind of preparation method rich in polyphenol buckwheat protein concentrate |
| CN106307445A (en) * | 2016-08-18 | 2017-01-11 | 青岛花帝食品配料有限公司 | Method for preparing beef bone essence |
| CN106905142A (en) * | 2017-04-18 | 2017-06-30 | 四川龙蟒福生科技有限责任公司 | A kind of isolation and purification method of S abscisic acids |
| CN109280683A (en) * | 2017-08-15 | 2019-01-29 | 广东丸美生物技术股份有限公司 | Preparation method of composite plant hydrolyzed protein peptide |
| CN107647285A (en) * | 2017-09-25 | 2018-02-02 | 贺州学院 | A kind of enzyme solution of oat |
| CN109400751A (en) * | 2019-01-07 | 2019-03-01 | 张家口康生物科技有限公司 | A kind of low content oat beta-glucan extracting method |
| CN111187763A (en) * | 2019-11-22 | 2020-05-22 | 武汉新华扬生物股份有限公司 | Complex enzyme preparation for oat hydrolysis and application thereof |
| CN112521525A (en) * | 2020-11-30 | 2021-03-19 | 福州美乐莲生物科技有限公司 | Production process for separating and purifying beta-glucan from oat bran |
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