CN106213508A - A kind of extracting method of ginsenoside - Google Patents
A kind of extracting method of ginsenoside Download PDFInfo
- Publication number
- CN106213508A CN106213508A CN201610582049.1A CN201610582049A CN106213508A CN 106213508 A CN106213508 A CN 106213508A CN 201610582049 A CN201610582049 A CN 201610582049A CN 106213508 A CN106213508 A CN 106213508A
- Authority
- CN
- China
- Prior art keywords
- ginsenoside
- extracting method
- described step
- radix ginseng
- weight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229940089161 ginsenoside Drugs 0.000 title claims abstract description 35
- 229930182494 ginsenoside Natural products 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 241000208340 Araliaceae Species 0.000 claims abstract description 22
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 22
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 22
- 235000008434 ginseng Nutrition 0.000 claims abstract description 22
- 239000000284 extract Substances 0.000 claims abstract description 17
- 238000005119 centrifugation Methods 0.000 claims abstract description 11
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 239000012982 microporous membrane Substances 0.000 claims abstract description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 9
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 9
- 239000011780 sodium chloride Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 30
- 239000011347 resin Substances 0.000 claims description 15
- 229920005989 resin Polymers 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000003463 adsorbent Substances 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 5
- 239000012141 concentrate Substances 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 244000199866 Lactobacillus casei Species 0.000 claims description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 3
- 238000010828 elution Methods 0.000 claims description 3
- 229940017800 lactobacillus casei Drugs 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 2
- 238000001228 spectrum Methods 0.000 claims 1
- 230000003068 static effect Effects 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 239000000178 monomer Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 5
- 238000002425 crystallisation Methods 0.000 description 4
- 230000008025 crystallization Effects 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000005374 membrane filtration Methods 0.000 description 2
- 235000013618 yogurt Nutrition 0.000 description 2
- VCNKUCWWHVTTBY-UHFFFAOYSA-N 18alpha-Oleanane Natural products C1CCC(C)(C)C2CCC3(C)C4(C)CCC5(C)CCC(C)(C)CC5C4CCC3C21C VCNKUCWWHVTTBY-UHFFFAOYSA-N 0.000 description 1
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- OORMXZNMRWBSTK-LGFJJATJSA-N dammarane Chemical compound C1CCC(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@H]([C@H](C)CCCC(C)C)[C@H]4CC[C@@H]3[C@]21C OORMXZNMRWBSTK-LGFJJATJSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- SIOMFBXUIJKTMF-UHFFFAOYSA-N hypoglauterpenic acid Natural products C1CC(O)C(C)(C)C2=CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C SIOMFBXUIJKTMF-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- BPAWXSVOAOLSRP-UHFFFAOYSA-N oleanane Natural products CCCCCCCCCCCCCCCC(=O)OC1CCC2(C)C(CCC3(C)C2CC=C4C5CC(C)(C)CCC5(C)C(O)CC34C)C1(C)C BPAWXSVOAOLSRP-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses the extracting method of a kind of ginsenoside, saline soak will be used after Radix Ginseng chip drying, clean, after initial strain fermentation, centrifugation, filtering with microporous membrane, continuous chromatography, then add secondary strain fermentation, be performing centrifugal separation on, filtering with microporous membrane obtains ginsenoside.The present invention extracts single ginsenoside by twice fermentable, and added value is high；Extract the ginsenoside after monomer still to sell as product, remarkable in economical benefits；Using large-scale production, yield is big.
Description
Technical field
The invention belongs to food compositions field, particularly relate to the extracting method of a kind of ginsenoside.
Background technology
Ginsenoside is the principle active component of Radix Ginseng, and the ginsenoside monomer the most clearly known there are about more than 40 kinds；?
Content in Radix Ginseng is about 4%.Ginsenoside has similar basic structure, all contains and is become four by 30 carbon atom arrangement
The gonane steroid core of individual ring, they are divided into two groups according to the difference of glycosyl framework: dammarane type and oleanane type.
The method preparing highly purified ginsenoside at present is mainly HPLC method, and the method cost is high, need complexity
Instrument and equipment, be difficult to amplify large-scale production.
Summary of the invention
In order to overcome the deficiencies in the prior art, it is an object of the invention to provide the extraction of the high ginsenoside of a kind of yield
Method.
For reaching above-mentioned purpose, the present invention is achieved by the following technical solutions.
The extracting method of a kind of ginsenoside, comprises the following steps:
1) by using saline soak 1 ~ 5min after Radix Ginseng chip drying, then wash, add the water of Radix Ginseng weight 3 ~ 5 times,
Add initial strain fermentation；
2) by step 1) gained Preliminary fermentation liquid centrifugation, taking clear liquid, clear liquid is degerming through filtering with microporous membrane；
3) by step 2) gained filtrate through continuous chromatography select separate to obtain extract；
4) by extract obtained for step 3) adding 3 ~ water of 5 times, add secondary strain fermentation；
5) by step 4) gained ferment in second time liquid centrifugation, taking clear liquid, clear liquid is degerming through filtering with microporous membrane；
6) ginsenoside is obtained by spray-dried for step 5) gained filtrate.
In the most described step 1), initial strain is the one in bacillus acidophilus, lactobacillus casei.
In the most described step 1), the consumption of strain is the 1 ~ 5% of Radix Ginseng weight, and fermentation temperature controls at 26 ~ 34 DEG C,
Ferment 24 ~ 72 hours.
The most described step 2), 4) in use aperture be the microporous filter membrane of 0.5 ~ 1 μm.
In the most described step 3), continuous chromatography uses resin continuous chromatography post, is 38 ~ 42% ethanol by volumetric concentration
Eluting, collects eluent, concentrate eluant, quiescent crystallization.
The most described resin is SP825 type adsorbent resin, SP700 type adsorbent resin, CHP20P type adsorbent resin
In one.
In the most described step 4), secondary strain is lactobacillus rhamnosus.
In the most described step 4), the consumption of strain is the 1 ~ 5% of extract weight, and fermentation temperature controls 26 ~ 34
DEG C, ferment 24 ~ 72 hours.
Beneficial effect: compared with prior art, it is an advantage of the current invention that:
1) extracting ginsenoside by secondary fermentable, added value is high；The more existing extractive technique of yield improves more than 50%；
2) ginsenoside can sell as product, remarkable in economical benefits；
3) using large-scale production, yield is big.
Detailed description of the invention
Below in conjunction with example, the present invention is described in further detail.
Bacillus acidophilus involved in the present invention, lactobacillus casei, lactobacillus rhamnosus Jun You Southern Yangtze University provide.
SP825 type adsorbent resin involved by the present invention, SP700 type adsorbent resin, CHP20P type absorption tree
Fat, is commercially available prod.
Embodiment 1
The extracting method of a kind of ginsenoside, comprises the following steps:
1) by after Radix Ginseng chip drying with 4 times of Radix Ginseng weight, weight percent concentration be 1.0% saline soak 3min, then
Clean 2 times with pure water, clean saline, add the water of Radix Ginseng weight 4 times, add weight be Radix Ginseng weight 3% addicted to yogurt bar
Bacterium is fermented, and fermentation temperature controls at 30 DEG C, ferments 48 hours；
2) by step 1) gained Preliminary fermentation liquid centrifugation under 5000 ~ 8000 r/min, taking clear liquid, clear liquid via hole diameter is
The filtering with microporous membrane of 0.5 ~ 1 μm is degerming；
3) by step 2) gained filtrate uses SP825 type adsorbent resin continuous chromatography post, is 40% ethanol elution by volumetric concentration,
Collect eluent, concentrate eluant, quiescent crystallization, obtain extract；
4) by extract obtained for the step 3) water adding 4 times, the lactobacillus rhamnosus sealing of weight is extract weight 3% is added
Fermentation, fermentation temperature controls at 30 DEG C, ferments 48 hours；
5) by step 4) gained ferment in second time liquid centrifugation under 7000 r/min, taking clear liquid, clear liquid via hole diameter is 0.5 μm
Filtering with microporous membrane is degerming；
6) ginsenoside is obtained by spray-dried for step 5) gained filtrate.Use the yield of ginsenoside obtained by this law with
HPLC method compares raising 55%.
Embodiment 2
The extracting method of a kind of ginsenoside, comprises the following steps:
1) by after Radix Ginseng chip drying with 2 times of Radix Ginseng weight, weight percent concentration be 1.5% saline soak 1min, then
Clean 1 time with pure water, clean saline, add the water of Radix Ginseng weight 3 times, add the cheese milk bar that weight is Radix Ginseng weight 1%
Bacterium sealing and fermenting, fermentation temperature controls at 34 DEG C, ferments 24 hours；
2) by step 1) gained Preliminary fermentation liquid centrifugation under 8000 r/min, taking clear liquid, clear liquid via hole diameter is the micro-of 1 μm
Hole membrane filtration is degerming；
3) by step 2) gained filtrate uses SP700 type adsorbent resin continuous chromatography post, is 38% ethanol elution by volumetric concentration,
Collect eluent, concentrate eluant, quiescent crystallization, obtain extract；
4) by extract obtained for the step 3) water adding 3 times, the lactobacillus rhamnosus sealing of weight is extract weight 1% is added
Fermentation, fermentation temperature controls at 34 DEG C, ferments 24 hours；
5) by step 4) gained ferment in second time liquid centrifugation under 8000 r/min, taking clear liquid, clear liquid via hole diameter is the micro-of 1 μm
Hole membrane filtration is degerming；
6) ginsenoside is obtained by spray-dried for step 5) gained filtrate.Use the yield of ginsenoside obtained by this law with
HPLC method compares raising 57%.
Embodiment 3
The extracting method of a kind of ginsenoside, comprises the following steps:
1) by after Radix Ginseng chip drying with 5 times of Radix Ginseng weight, weight percent concentration be 0.5% saline soak 5min, then
Clean 3 times with pure water, clean saline, add the water of Radix Ginseng weight 5 times, add weight be Radix Ginseng weight 5% addicted to yogurt bar
Bacterium sealing and fermenting, fermentation temperature controls at 26 DEG C, ferments 72 hours；
2) by step 1) gained Preliminary fermentation liquid centrifugation under 5000 r/min, taking clear liquid, clear liquid via hole diameter is 0.5 μm
Filtering with microporous membrane is degerming；
3) by step 2) gained filtrate uses CHP20P type adsorbent resin continuous chromatography post, is that 42% ethanol is washed by volumetric concentration
De-, collect eluent, concentrate eluant, quiescent crystallization, obtain extract；
4) by extract obtained for the step 3) water adding 5 times, the lactobacillus rhamnosus sealing of weight is extract weight 5% is added
Fermentation, fermentation temperature controls at 26 DEG C, ferments 72 hours；
5) by step 4) gained ferment in second time liquid centrifugation under 5000 r/min, taking clear liquid, clear liquid via hole diameter is 0.5 μm
Filtering with microporous membrane is degerming；
6) ginsenoside is obtained by spray-dried for step 5) gained filtrate.Use the yield of ginsenoside obtained by this law with
HPLC method compares raising 56%.
The present invention is illustrated according to above-described embodiment, it will be appreciated that above-described embodiment limits this most in any form
Invention, the technical scheme that all employing equivalents or equivalent transformation mode are obtained, within all falling within protection scope of the present invention.
Claims (9)
1. the extracting method of a ginsenoside, it is characterised in that comprise the following steps:
1) by using saline soak 1 ~ 5min after Radix Ginseng chip drying, then wash, add the water of Radix Ginseng weight 3 ~ 5 times,
Add initial strain fermentation；
2) by step 1) gained Preliminary fermentation liquid centrifugation, taking clear liquid, clear liquid is degerming through filtering with microporous membrane；
3) by step 2) gained filtrate through continuous chromatography select separate to obtain extract；
4) by extract obtained for step 3) adding 3 ~ water of 5 times, add secondary strain fermentation；
5) by step 4) gained ferment in second time liquid centrifugation, taking clear liquid, clear liquid is degerming through filtering with microporous membrane；
6) ginsenoside is obtained by spray-dried for step 5) gained filtrate.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that initial bacterium in described step 1)
Plant as the one in bacillus acidophilus, lactobacillus casei.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that strain in described step 1)
Consumption is the 1 ~ 5% of Radix Ginseng weight, and fermentation temperature controls at 26 ~ 34 DEG C, ferments 24 ~ 72 hours.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that described step 1) is mesohalobic
Weight percent concentration is 0.5 ~ 1.5%, and consumption is 2 ~ 5 times of Radix Ginseng weight.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that described step 2), 4) in adopt
With the microporous filter membrane that aperture is 0.5 ~ 1 μm.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that continuous color in described step 3)
Spectrum uses resin continuous chromatography post, is 38 ~ 42% ethanol elutions by volumetric concentration, collects eluent, concentrate eluant, static knot
Brilliant.
The extracting method of a kind of ginsenoside the most according to claim 6, it is characterised in that described resin is SP825 type
One in adsorbent resin, SP700 type adsorbent resin, CHP20P type adsorbent resin.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that secondary bacterium in described step 4)
Plant as lactobacillus rhamnosus.
The extracting method of a kind of ginsenoside the most according to claim 1, it is characterised in that strain in described step 4)
Consumption is the 1 ~ 5% of extract weight, and fermentation temperature controls at 26 ~ 34 DEG C, ferments 24 ~ 72 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610582049.1A CN106213508A (en) | 2016-07-22 | 2016-07-22 | A kind of extracting method of ginsenoside |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610582049.1A CN106213508A (en) | 2016-07-22 | 2016-07-22 | A kind of extracting method of ginsenoside |
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| CN106213508A true CN106213508A (en) | 2016-12-14 |
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| CN201610582049.1A Pending CN106213508A (en) | 2016-07-22 | 2016-07-22 | A kind of extracting method of ginsenoside |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107779475A (en) * | 2017-09-30 | 2018-03-09 | 安徽利民生物科技股份有限公司 | A kind of extracting method of sponge gourd saponin(e |
| CN108403741A (en) * | 2018-04-18 | 2018-08-17 | 佛山市飞程信息技术有限公司 | A kind of method of microwave radiation exaraction ginsenoside |
| CN109096356A (en) * | 2018-08-30 | 2018-12-28 | 佛山市欧若拉生物科技有限公司 | A kind of ginsenoside preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1723912A (en) * | 2005-07-14 | 2006-01-25 | 中国医药研究开发中心有限公司 | Medicinal composition contg. ginseng secondary glycosides, prepn. method and application thereof |
| CN101032535A (en) * | 2006-12-22 | 2007-09-12 | 吉林人参研究院 | Extracting purified ginsenoside from leaves of Panax quinquefolium and ginseng at the same time and the preparing method thereof |
| CN102846685A (en) * | 2012-09-25 | 2013-01-02 | 吉林省宏久生物科技股份有限公司 | Preparation technology and method for improving total content of ginseng saponin through fermentation method |
-
2016
- 2016-07-22 CN CN201610582049.1A patent/CN106213508A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1723912A (en) * | 2005-07-14 | 2006-01-25 | 中国医药研究开发中心有限公司 | Medicinal composition contg. ginseng secondary glycosides, prepn. method and application thereof |
| CN101032535A (en) * | 2006-12-22 | 2007-09-12 | 吉林人参研究院 | Extracting purified ginsenoside from leaves of Panax quinquefolium and ginseng at the same time and the preparing method thereof |
| CN102846685A (en) * | 2012-09-25 | 2013-01-02 | 吉林省宏久生物科技股份有限公司 | Preparation technology and method for improving total content of ginseng saponin through fermentation method |
Non-Patent Citations (1)
| Title |
|---|
| 宋艳: "人参（根）生物转化研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107779475A (en) * | 2017-09-30 | 2018-03-09 | 安徽利民生物科技股份有限公司 | A kind of extracting method of sponge gourd saponin(e |
| CN108403741A (en) * | 2018-04-18 | 2018-08-17 | 佛山市飞程信息技术有限公司 | A kind of method of microwave radiation exaraction ginsenoside |
| CN109096356A (en) * | 2018-08-30 | 2018-12-28 | 佛山市欧若拉生物科技有限公司 | A kind of ginsenoside preparation method |
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Application publication date: 20161214 |