CN109096356A - A kind of ginsenoside preparation method - Google Patents
A kind of ginsenoside preparation method Download PDFInfo
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- CN109096356A CN109096356A CN201811002123.3A CN201811002123A CN109096356A CN 109096356 A CN109096356 A CN 109096356A CN 201811002123 A CN201811002123 A CN 201811002123A CN 109096356 A CN109096356 A CN 109096356A
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- ginseng
- strain
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- ginsenoside
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07J—STEROIDS
- C07J17/00—Normal steroids containing carbon, hydrogen, halogen or oxygen, having an oxygen-containing hetero ring not condensed with the cyclopenta(a)hydrophenanthrene skeleton
- C07J17/005—Glycosides
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of ginsenoside preparation methods, including fresh ginseng, initial strain, secondary strain, compound biological enzyme and low-carbon alcohols, the utility model has the advantages that the present invention uses fresh ginseng, whole drying is carried out first, it is dried in the case where not destroying its crust so that ginseng moisture will not carry away the corpuscle in it when its moisture evaporates, save its main matter, ginseng after drying, which is first sliced, to crush again can make ginseng when crushing, uniform particle sizes, ginseng granules enzymatic hydrolysis can be destroyed into its fiber mechanism, the substances such as its interior starch of cell wall lysis, avoid the case where adverse effect is caused to purification in subsequent handling appearance, low-carbon alcohols are to extract compound biological enzyme, the fermentation of initial strain and secondary strain can further dispel the impurity in ginseng, process of the invention is simple, operation side Just.
Description
Technical field
The invention discloses a kind of ginsenoside preparation methods, belong to saponin(e preparation preparation technical field.
Background technique
Ginsenoside is a kind of steroid compound, triterpenoid saponin.It is primarily present in Panax medicinal material.Ginsenoside quilt
The active constituent being considered as in ginseng, thus become the target of research.Because ginsenoside affects multiple metabolic pathway, institute
It is also complicated with its efficiency, and the monomer component of various ginsenosides is difficult to separate.
In the prior art, catalyst or biological inoculum type used in the abstraction process of ginsenoside are excessively numerous
It is miscellaneous, cause it to filter out process comparatively also troublesome.
Summary of the invention
It is used in the abstraction process of ginsenoside to urge the technical problem to be solved by the present invention is to overcome in the prior art
Agent or biological inoculum type are excessively many and diverse, cause it to filter out process comparatively also troublesome problem, provide a kind of people
Join saponin(e preparation method, to solve the above problems.
To achieve the above object, the invention provides the following technical scheme: a kind of ginsenoside preparation method, including Freshman
Ginseng, initial strain, secondary strain, compound biological enzyme and low-carbon alcohols, preparation step:
(1) qualified fresh ginseng will be examined to wash, then dried, until moisture content is lower than 2%；
(2) it by the ginseng defoliation after washing, drying, slice, then dries, slice thickness 2mm；
(3) ginseng slice is crushed, obtains ginseng granules, enzyme is then carried out to ginseng granules using compound biological enzyme
Solution, filtering obtain enzymatic hydrolysis material；
(4) material extracting in water will be digested, filtering obtains filter residue, and Aqueous extracts retain；
(5) filter residue is repeatedly extracted using low-carbon alcohols, obtains multiple alcohol extract；
(6) merge Aqueous extracts and multiple alcohol extract, obtain Ginseng extract；
(7) a certain amount of water is added in Ginseng extract, until solution weight is 4 times of script ginseng weight, added
Initial strain ferments, and the dosage of strain is the 5% of ginseng weight, and fermentation temperature is controlled at 30-34 DEG C, ferments 56 hours,
Obtain Preliminary fermentation liquid；
(8) Preliminary fermentation liquid is centrifugated, and takes clear liquid, clear liquid obtains filtrate through filtering with microporous membrane degerming；
(9) filtrate separates to obtain extract through continuous chromatography selection, and extract adds 4 times of water, adds secondary strain fermentation, strain
Dosage be extract weight 4%, fermentation temperature control at 30-34 DEG C, ferment 64 hours, obtain secondary fermentation liquid；
(10) filtrate is spray-dried obtains ginsenoside.
Preferably, the initial strain is lactobacillus acidophilus.
Preferably, the secondary strain is Lactobacillus rhamnosus.
Preferably, the compound biological enzyme includes cellulase, amylase and pectase.
Preferably, when the filter residue carries out multiple alcohol extracting, successively using 4 times 35vol.%, 55vol.% and
The low-carbon alcohol extracting of 75vol.% is multiple, and each extraction time is 2h, and the alcohol extract repeatedly extracted retains, wherein alcohol extracting is adopted
It is extracted with adverse current.
Preferably, the low-carbon alcohols are C3 low-carbon alcohols.
Preferably, the continuous chromatography uses resin continuous chromatography column, it is 38~42% ethanol elutions with volumetric concentration,
Collect eluent, concentrate eluant, quiescent crystallization.
Preferably, the miillpore filter uses aperture for 0.5~1 μm.
Compared with prior art, beneficial effects of the present invention are as follows:
The present invention uses fresh ginseng, carries out whole drying first, in the case where not destroying its crust to its into
Row drying saves its main matter so that ginseng moisture will not carry away corpuscle in it when its moisture evaporates, will
Ginseng after drying, which is first sliced to crush again, can make ginseng when crushing, uniform particle sizes, and ginseng granules enzymatic hydrolysis can be destroyed
The substances such as its fiber mechanism, cell wall lysis its interior starch, avoid and cause dysgenic feelings to purification in subsequent handling
Condition occurs, and low-carbon alcohols can be dispelled further in ginseng to extract compound biological enzyme, the fermentation of initial strain and secondary strain
Impurity, process of the invention is simple, easy to operate.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
A kind of ginsenoside preparation method, including fresh ginseng, initial strain, secondary strain, compound biological enzyme and low-carbon
Alcohol, preparation step:
(1) qualified fresh ginseng will be examined to wash, then dried, until moisture content is lower than 2%；
(2) it by the ginseng defoliation after washing, drying, slice, then dries, slice thickness 2mm；
(3) ginseng slice is crushed, obtains ginseng granules, enzyme is then carried out to ginseng granules using compound biological enzyme
Solution, filtering obtain enzymatic hydrolysis material；
(4) material extracting in water will be digested, filtering obtains filter residue, and Aqueous extracts retain；
(5) filter residue is repeatedly extracted using low-carbon alcohols, obtains multiple alcohol extract；
(6) merge Aqueous extracts and multiple alcohol extract, obtain Ginseng extract；
(7) a certain amount of water is added in Ginseng extract, until solution weight is 4 times of script ginseng weight, added
Initial strain ferments, and the dosage of strain is the 5% of ginseng weight, and fermentation temperature is controlled at 30-34 DEG C, ferments 56 hours,
Obtain Preliminary fermentation liquid；
(8) Preliminary fermentation liquid is centrifugated, and takes clear liquid, clear liquid obtains filtrate through filtering with microporous membrane degerming；
(9) filtrate separates to obtain extract through continuous chromatography selection, and extract adds 4 times of water, adds secondary strain fermentation, strain
Dosage be extract weight 4%, fermentation temperature control at 30-34 DEG C, ferment 64 hours, obtain secondary fermentation liquid；
(10) filtrate is spray-dried obtains ginsenoside.
Preferably, the initial strain is lactobacillus acidophilus.
Preferably, the secondary strain is Lactobacillus rhamnosus.
Preferably, the compound biological enzyme includes cellulase, amylase and pectase.
Preferably, when the filter residue carries out multiple alcohol extracting, successively using 4 times 35vol.%, 55vol.% and
The low-carbon alcohol extracting of 75vol.% is multiple, and each extraction time is 2h, and the alcohol extract repeatedly extracted retains, wherein alcohol extracting is adopted
It is extracted with adverse current.
Preferably, the low-carbon alcohols are C3 low-carbon alcohols.
Preferably, the continuous chromatography uses resin continuous chromatography column, it is 38~42% ethanol elutions with volumetric concentration,
Collect eluent, concentrate eluant, quiescent crystallization.
Preferably, the miillpore filter uses aperture for 0.5~1 μm.
Specifically, the present invention uses fresh ginseng, whole drying is carried out first, in the case where not destroying its crust
It is dried so that ginseng moisture will not carry away corpuscle in it when its moisture evaporates, saves its main object
Matter, the ginseng after drying, which is first sliced, to crush again can make ginseng when crushing, uniform particle sizes, can be with by ginseng granules enzymatic hydrolysis
The substances such as its fiber mechanism, cell wall lysis its interior starch are destroyed, avoids and adverse effect is caused to purification in subsequent handling
The case where occur, low-carbon alcohols can further dispel people to extract compound biological enzyme, the fermentation of initial strain and secondary strain
Impurity in ginseng, process of the invention is simple, easy to operate.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of ginsenoside preparation method, which is characterized in that including fresh ginseng, initial strain, secondary strain, compound bio
Enzyme and low-carbon alcohols, preparation step:
(1) qualified fresh ginseng will be examined to wash, then dried, until moisture content is lower than 2%；
(2) it by the ginseng defoliation after washing, drying, slice, then dries, slice thickness 2mm；
(3) ginseng slice is crushed, obtains ginseng granules, then ginseng granules digested using compound biological enzyme, mistake
Filter obtains enzymatic hydrolysis material；
(4) material extracting in water will be digested, filtering obtains filter residue, and Aqueous extracts retain；
(5) filter residue is repeatedly extracted using low-carbon alcohols, obtains multiple alcohol extract；
(6) merge Aqueous extracts and multiple alcohol extract, obtain Ginseng extract；
(7) a certain amount of water is added in Ginseng extract, until solution weight is 4 times of script ginseng weight, added initial
Strain ferments, and the dosage of strain is the 5% of ginseng weight, and fermentation temperature is controlled at 30-34 DEG C, is fermented 56 hours, obtains just
Originate zymotic fluid；
(8) Preliminary fermentation liquid is centrifugated, and takes clear liquid, clear liquid obtains filtrate through filtering with microporous membrane degerming；
(9) filtrate separates to obtain extract through continuous chromatography selection, and extract adds 4 times of water, adds secondary strain fermentation, the use of strain
Amount is the 4% of extract weight, and fermentation temperature controls at 30-34 DEG C, ferments 64 hours, obtain secondary fermentation liquid；
(10) filtrate is spray-dried obtains ginsenoside.
2. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the initial strain is acidophilus cream
Bacillus.
3. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the secondary strain is rhamnose
Lactobacillus.
4. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the compound biological enzyme includes fibre
Tie up plain enzyme, amylase and pectase.
5. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the filter residue carries out multiple alcohol extracting
Successively multiple using the low-carbon alcohol extracting of 4 times 35vol.%, 55vol.% and 75vol.% when taking, each extraction time is
2h, the alcohol extract repeatedly extracted retain, wherein alcohol extracting is extracted using adverse current.
6. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the low-carbon alcohols are C3 low-carbon
Alcohol.
7. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the continuous chromatography uses resin
Continuous chromatography column is 38~42% ethanol elutions with volumetric concentration, collects eluent, concentrate eluant, quiescent crystallization.
8. a kind of ginsenoside preparation method according to claim 1, it is characterised in that: the miillpore filter uses aperture
It is 0.5~1 μm.
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| CN201811002123.3A CN109096356A (en) | 2018-08-30 | 2018-08-30 | A kind of ginsenoside preparation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170885A (en) * | 2018-09-12 | 2019-01-11 | 北京化工大学 | A method of extracting ginseng effective component |
| CN112168750A (en) * | 2020-08-31 | 2021-01-05 | 长春罗兰生物科技有限公司 | Preparation method of ginsenoside extract and application of ginsenoside extract in skin care product |
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| KR20050053048A (en) * | 2003-12-02 | 2005-06-08 | 주식회사 동원고려인삼 | Process for preparing an red ginseng extract containing a large amount of ginsenoside rg3 |
| CN103142669A (en) * | 2013-03-07 | 2013-06-12 | 安徽丰润生物技术有限公司 | Enzyme method for extracting ginsenoside and obtained ginsenoside extractive |
| CN106213508A (en) * | 2016-07-22 | 2016-12-14 | 江苏丹枫神源生物科技有限公司 | A kind of extracting method of ginsenoside |
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2018
- 2018-08-30 CN CN201811002123.3A patent/CN109096356A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050053048A (en) * | 2003-12-02 | 2005-06-08 | 주식회사 동원고려인삼 | Process for preparing an red ginseng extract containing a large amount of ginsenoside rg3 |
| CN103142669A (en) * | 2013-03-07 | 2013-06-12 | 安徽丰润生物技术有限公司 | Enzyme method for extracting ginsenoside and obtained ginsenoside extractive |
| CN106213508A (en) * | 2016-07-22 | 2016-12-14 | 江苏丹枫神源生物科技有限公司 | A kind of extracting method of ginsenoside |
Non-Patent Citations (1)
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| 王中立等: "人参皂苷的提取及总皂苷纯化工艺的研究进展", 《世界科学技术-中医药现代化》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170885A (en) * | 2018-09-12 | 2019-01-11 | 北京化工大学 | A method of extracting ginseng effective component |
| CN112168750A (en) * | 2020-08-31 | 2021-01-05 | 长春罗兰生物科技有限公司 | Preparation method of ginsenoside extract and application of ginsenoside extract in skin care product |
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