CN109206336B - Method for preparing ceramide from rice bran by fermentation method - Google Patents
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- CN109206336B CN109206336B CN201811208330.4A CN201811208330A CN109206336B CN 109206336 B CN109206336 B CN 109206336B CN 201811208330 A CN201811208330 A CN 201811208330A CN 109206336 B CN109206336 B CN 109206336B
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- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 title claims abstract description 74
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 title claims abstract description 74
- 229940106189 ceramide Drugs 0.000 title claims abstract description 74
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 title claims abstract description 74
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 title claims abstract description 74
- 238000000855 fermentation Methods 0.000 title claims abstract description 64
- 230000004151 fermentation Effects 0.000 title claims abstract description 64
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 59
- 235000009566 rice Nutrition 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 55
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 58
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 42
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 40
- 241000222336 Ganoderma Species 0.000 claims abstract description 24
- 239000007787 solid Substances 0.000 claims abstract description 24
- 239000012535 impurity Substances 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 7
- 238000010298 pulverizing process Methods 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000002156 mixing Methods 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 12
- 241001489091 Ganoderma sinense Species 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 239000008223 sterile water Substances 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 210000002421 cell wall Anatomy 0.000 claims description 5
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- 238000005520 cutting process Methods 0.000 claims description 4
- 241001480597 Ganoderma tsugae Species 0.000 claims description 3
- 230000008569 process Effects 0.000 abstract description 8
- 238000000605 extraction Methods 0.000 abstract description 7
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- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 12
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 6
- 239000003960 organic solvent Substances 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 241000640636 Ganoderma leucocontextum Species 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
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- 239000003208 petroleum Substances 0.000 description 3
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- 108010059892 Cellulase Proteins 0.000 description 2
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- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 206010003645 Atopy Diseases 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
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- 241001149422 Ganoderma applanatum Species 0.000 description 1
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- 229920002488 Hemicellulose Polymers 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C231/00—Preparation of carboxylic acid amides
- C07C231/22—Separation; Purification; Stabilisation; Use of additives
- C07C231/24—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
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- General Chemical & Material Sciences (AREA)
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Abstract
The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for preparing ceramide from rice bran by a fermentation method. The method comprises the following steps: removing impurities from testa oryzae, pulverizing, sieving, adding Ganoderma into testa oryzae powder, and fermenting; separating the fermentation liquor, taking clear liquid to obtain ceramide solution, and concentrating and crystallizing the obtained ceramide solution to obtain ceramide solid. The invention utilizes the ganoderma lucidum to ferment the rice bran, which not only can obviously improve the dissolution of ceramide in the rice bran, but also can generate a certain amount of ceramide, and the invention does not need any derivatization process, has simple operation, convenient extraction, short period, environmental protection, no regional climate limitation and can obviously reduce the economic cost.
Description
Technical Field
The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for preparing ceramide from rice bran by a fermentation method.
Background
Ceramide (Ceramide), also known as molecular nail and Serlomex, is a signal transduction substance widely existing in eukaryotic cells, and mainly exists in the form of structural components of cell membranes or intracellular free molecules. Ceramide is an active ingredient of medicines, foods and cosmetics which has been focused in recent years, and it is reported that the biological function of ceramide mainly includes the following aspects: firstly, cell adhesion; ② regulating cell proliferation and differentiation; inducing apoptosis; regulating protein secretion; inflammation. The cosmetic composition can be used as a barrier to prevent water loss, and has the effects of protecting and repairing skin, improving human immunity, preventing and resisting cancer, and the like.
The rice bran is the surface layer attached to the unpolished rice after unhulled rice, and consists of the epicarp, mesocarp, crosslinked layer, seed coat and aleurone layer produced in the process of whitening, 64 percent of rice nutrients and more than 90 percent of essential elements for human body are concentrated in the rice bran, the chemical components of the rice bran are mainly saccharides, fat and protein, and the rice bran is known as a "Tianciyuan" in foreign countries. The ceramide content in rice bran is high, and as in "a composition having anti-atopic activity containing ceramide extracted from rice bran as an active ingredient [ (publication) No. CN105311041A ]" and "a method of extracting high purity ceramide from rice bran [ (publication) No. CN106008253B ]", both methods of preparing ceramide from rice bran by heating and extracting for a long time using an organic solvent such as ethanol, petroleum ether, etc., but because of the cell wall structure of plant cells, substances in the wall are not easily separated and extracted, so that energy consumption is large, the amount of organic solvent is large, and the extraction yield is low "a process of preparing ceramide by fermentation [ (publication) No. CN106119310A ]", the ceramide is prepared by fermentation using penicillium purpurogenum, although the process of preparing ceramide is simplified, but methanol is used as a solvent in the preparation process, residual methanol can cause harm to human bodies, and the production period of the invention is longer.
In order to obtain ceramide more efficiently, a preparation method which is high in efficiency, low in cost, simple in separation step and environment-friendly is urgently needed.
Disclosure of Invention
Aiming at the technical problems, the invention provides the method for preparing the ceramide from the rice bran by the fermentation method, the rice bran is used as the raw material, the ganoderma lucidum is used for fermenting the rice bran, the dissolution of the ceramide in the rice bran can be obviously improved, and the ganoderma lucidum can also generate a certain amount of ceramide, so that any derivatization process is not needed, the operation is simple, the extraction is convenient, the period is short, and the method is green and environment-friendly.
The invention is realized by the following technical scheme:
a method for preparing ceramide from rice bran by fermentation method comprises fermenting rice bran with Ganoderma to degrade cell wall of rice bran; homogenizing and breaking the wall after fermentation to obtain wall-broken homogenate, and removing impurities to obtain ceramide solution.
Further, when the rice bran is fermented by using the ganoderma lucidum, the fermentation conditions need to be controlled so that the ganoderma lucidum can be deeply fermented.
Further, in order to make ganoderma lucidum carry out submerged fermentation on rice bran, the specific fermentation conditions are as follows: mixing the ganoderma seed liquid and the rice bran raw material powder according to the mass ratio of (1-5) to 1, putting the mixture into a fermentation tank, fermenting for 2-5 days at the temperature of 28 +/-2 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.010-0.025 mol/L, wherein the fermentation condition can ensure that ganoderma lucidum can grow well in the fermentation tank, rich hyphae are produced, the ganoderma lucidum mycelia are proliferated in large quantity, and ceramide compounds can be enriched.
Further, the preparation method of the ganoderma lucidum seed liquid comprises the following steps:
activating strains: inoculating Ganoderma mycelia with culture medium cut from the slant with an inoculating hook into the middle of solid culture medium, and culturing at 28 + -2 deg.C for 3-5 days;
preparing a seed solution: smashing the solid culture medium with the ganoderma lucidum by using an inoculation hook when the ganoderma lucidum mycelia overgrow the surface of the solid culture medium, and then adding a proper amount of sterile water to obtain ganoderma lucidum seed liquid, wherein the use amount of the sterile water is 2-5 times of the volume of the solid culture medium.
Further, the method for homogenizing and breaking the wall after fermentation specifically comprises the following steps: after fermentation, adding distilled water with equal weight into the fermented product, mixing, homogenizing for 1-5 times at normal temperature and 70-120Mpa by high pressure homogenizer until no obvious Ganoderma mycelium exists, and obtaining wall-broken homogenate.
Further, the method for removing impurities comprises the following steps: removing impurities in the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
Further, before the rice bran is fermented by ganoderma lucidum, the rice bran needs to be treated, and the treatment method comprises the following steps: removing impurities from testa oryzae, pulverizing, and sieving.
Further, the Ganoderma lucidum is one of Ganoderma lucidum (G.lucidum), Ganoderma sinense (G.sinense), Ganoderma tsugae (G.tsugae Murr.), Ganoderma sinense (G.lingzhi), Ganoderma sinense (G.leuccontextum), and Ganoderma sinense (G.leuccontextum).
Further, mixing the prepared ceramide solution with an ethanol solution with the mass fraction of 95% at a normal temperature according to the weight ratio of 1:5, uniformly stirring, standing overnight, filtering out precipitates, concentrating under reduced pressure at the temperature of 30-40 ℃ under the pressure of 0.001-0.01 MPa until the solution is turbid, and cooling and crystallizing under normal pressure to obtain a ceramide solid, wherein the mass fraction of ceramide in the ceramide solid is 0.1-1.0%.
Further, the method specifically comprises the following steps:
raw material treatment: removing impurities from testa oryzae, pulverizing, and sieving;
activating strains: cutting 0.5-1cm from the inclined plane with inoculating hook2Inoculating the slant Ganoderma mycelia with the culture medium into the middle part of the solid culture medium, and culturing at 28 + -2 deg.C for 3-5 days.
Preparing a seed solution: mashing the solid culture medium with Ganoderma with inoculating hook, and adding appropriate amount of sterile water to obtain Ganoderma seed solution.
Fermentation culture: mixing the ganoderma lucidum seed liquid and the rice bran raw material powder according to the mass ratio of (1-5) to 1, putting the mixture into a fermentation tank, and fermenting for 2-5 days under the condition that ganoderma lucidum grows optimally (namely the temperature is 28 +/-2 ℃, and the oxygen concentration of the space in the fermentation tank is 0.010-0.025 mol/L);
homogenizing and breaking walls: after fermentation, adding distilled water with equal weight into the fermented product, mixing uniformly, and homogenizing for 2 times at normal temperature and 90Mpa by a high-pressure homogenizer to obtain wall-broken homogenate;
removing impurities: removing impurities of the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
Wherein, the Ganoderma (Ganoderma) is an important dual-purpose fungus for medicine and food in basidiomycetes, polyporaceae and Ganoderma, and is one of the traditional and rare medicinal materials in China. During the deep fermentation of ganoderma, mycelia are proliferated in a large amount, not only can polysaccharide, triterpene and other active substances be generated, but also abundant extracellular enzyme systems such as cellulase, hemicellulase, ligninase, protease, amylase and the like can be generated, the enzymes can perform various extracellular biochemical reactions outside cells, cell walls can be effectively degraded, the dissolution of substances in the walls can be promoted, and meanwhile, ceramide compounds can be enriched due to the large proliferation of the ganoderma mycelia. Therefore, the method for preparing the ceramide from the rice bran by the ganoderma lucidum fermentation method can obtain higher ceramide yield.
The invention has the beneficial technical effects that:
according to the method, the rice bran is fermented by adopting the ganoderma lucidum, and the extracellular enzyme system (comprising cellulase, hemicellulose, ligninase, protease and the like) generated by the ganoderma lucidum can effectively degrade the cell wall of the rice bran, so that the dissolution of ceramide is remarkably improved, meanwhile, the ganoderma lucidum can proliferate in a large quantity, and a certain amount of ceramide can be generated.
The method has the advantages of simple operation, convenient extraction, no use of organic solvents such as ethanol, petroleum ether, methanol and the like in the whole process, safe and pollution-free products, and capability of greatly reducing the production cost and environmental pollution and promoting the large-scale industrial production of the ceramide by utilizing the method to produce the ceramide. In addition, the method adopts ganoderma lucidum to carry out submerged fermentation on the rice bran, the fermentation time is 2 to 5 days, and the fermentation period is short.
The ceramide solution prepared by the method is decompressed and concentrated, and the mass fraction of ceramide in the obtained ceramide solid is 0.1-1.0%.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The ganoderma lucidum used in the following examples is a strain of the following species: ganoderma lucidum (Ganoderma lucidum), Ganoderma sinense (G.sinense), Ganoderma Applanatum (G.aplatatum), and Ganoderma leucocontextum (G.leucocontextum). Each strain was purchased from China general microbiological culture Collection center (CGMCC).
Aiming at the problems that in the prior art, organic solvents such as methanol, ethanol, petroleum ether and the like are adopted to prepare ceramide from rice bran, substances in walls are not easy to separate and extract, the energy consumption in the extraction process is large, the use amount of the organic solvents is large, and the extraction yield is low. The embodiment of the invention provides a method for preparing ceramide from rice bran by a fermentation method.
Example 1
A method for preparing ceramide from rice bran by a fermentation method specifically comprises the following steps:
(1) raw material treatment: drying rice bran in an oven at 80 deg.C, removing impurities, pulverizing, and sieving with 20-40 mesh sieve;
(2) activating strains: cutting Ganoderma (G.lucidum) with inoculating hook to 1cm from the inclined plane2Inoculating slant mycelia with culture medium into the middle of solid culture medium, and culturing at 30 deg.C for 5 days;
(3) preparing a seed solution: mashing the solid culture medium with Ganoderma with inoculating hook, and adding appropriate amount of sterile water to obtain Ganoderma seed solution;
(4) fermentation culture: mixing the ganoderma lucidum seed liquid and the rice bran raw material powder according to the mass ratio of 5:1, putting the mixture into a fermentation tank, culturing for 5 days at the temperature of 30 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.025 mol/L;
(5) homogenizing and breaking walls: after fermentation, adding distilled water with equal weight into the fermented product, mixing, and homogenizing for 2 times at normal temperature and 100Mpa by high pressure homogenizer to obtain wall-broken homogenate;
(6) removing impurities: removing impurities of the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
In this example, the ceramide solution obtained is concentrated and crystallized to obtain a ceramide solid, which is specifically prepared by: mixing the prepared ceramide solution with an ethanol solution with the mass fraction of 95% at a weight ratio of 1:5 at normal temperature, uniformly stirring, standing overnight, filtering out precipitates, concentrating under reduced pressure at 30-40 ℃ under the pressure of 0.001-0.01 MPa until the solution is turbid, cooling and crystallizing under normal pressure to obtain a ceramide solid, wherein the mass fraction of the ceramide in the obtained ceramide solid is 10%.
Example 2
A method for preparing ceramide from rice bran by a fermentation method specifically comprises the following steps:
(1) raw material treatment: drying rice bran in an oven at 80 deg.C, removing impurities, pulverizing, and sieving with 20-40 mesh sieve;
(2) activating strains: cutting Ganoderma leucocontextum (G.leucocotextum) with inoculating hook to 1cm from slant2Inoculating slant mycelia with culture medium into the middle of solid culture medium, and culturing at 28 deg.C for 5 days;
(3) preparing a seed solution: mashing the solid culture medium with Ganoderma with inoculating hook, and adding appropriate amount of sterile water to obtain Ganoderma seed solution;
(4) fermentation culture: mixing the ganoderma lucidum seed liquid and the rice bran raw material powder according to the mass ratio of 3:1, putting the mixture into a fermentation tank, culturing for 5 days at the temperature of 28 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.02 mol/L;
(5) homogenizing and breaking walls: after fermentation, adding distilled water with equal weight into the fermented product, mixing, and homogenizing for 5 times at room temperature and 70MPa with a high-pressure homogenizer to obtain wall-broken homogenate;
(6) removing impurities: removing impurities of the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
(7) Concentrating the ceramide solution under reduced pressure to obtain ceramide stock solution with total ceramide content of 0.5%.
According to the method, the rice bran is fermented by the ganoderma lucidum, so that the dissolution of ceramide in the rice bran can be remarkably improved, and the ganoderma lucidum can also generate a certain amount of ceramide, so that any derivatization process is not needed, the operation is simple, and the extraction is convenient; the fermentation period is short, the effect is quick, and the method is not limited by regional climate; the process for producing the ceramide can greatly reduce the dosage of the organic solvent, reduce the environmental influence and reduce the economic cost.
Claims (8)
1. A method for preparing ceramide from rice bran by fermentation is characterized in that the rice bran is used as a raw material, ganoderma lucidum is adopted to ferment the rice bran so as to degrade cell walls of the rice bran, and meanwhile, the ganoderma lucidum can propagate in a large quantity to generate a certain amount of ceramide; homogenizing and breaking the wall after fermentation to obtain wall-broken homogenate, and removing impurities to obtain a ceramide solution;
when the rice bran is fermented by adopting the ganoderma lucidum, the fermentation conditions are controlled so that the ganoderma lucidum is subjected to submerged fermentation;
in order to make ganoderma lucidum carry out submerged fermentation on rice bran, the specific fermentation conditions are as follows: mixing the ganoderma lucidum seed liquid and the rice bran raw material powder according to the mass ratio of (1-5) to 1, putting the mixture into a fermentation tank, fermenting for 2-5 days at the temperature of 28 +/-2 ℃, and controlling the oxygen concentration of the space in the fermentation tank to be 0.010-0.025 mol/L.
2. The method for preparing ceramide from rice bran by fermentation according to claim 1, wherein the ganoderma lucidum seed solution is prepared by the method comprising:
activating strains: inoculating Ganoderma mycelia with culture medium cut from the slant with an inoculating hook into the middle of solid culture medium, and culturing at 28 + -2 deg.C for 3-5 days;
preparing a seed solution: mashing the solid culture medium with Ganoderma with inoculating hook, and adding appropriate amount of sterile water to obtain Ganoderma seed solution, wherein the amount of sterile water is 2-5 times of the volume of the solid culture medium.
3. The method for preparing ceramide from rice bran by fermentation according to claim 1, wherein the homogenate is broken after fermentation by the following specific method: after fermentation, adding distilled water with equal weight into the fermented product, mixing, and homogenizing for 1-5 times at 70-120Mpa at normal temperature by high pressure homogenizer to obtain wall-broken homogenate.
4. The method for preparing ceramide from rice bran through fermentation according to claim 1, wherein the impurities are removed by: removing impurities in the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
5. The method of claim 1, wherein the rice bran is treated by a fermentation process prior to fermentation with the ganoderma lucidum, the fermentation process comprising: removing impurities from testa oryzae, pulverizing, and sieving.
6. The method for preparing ceramide from rice bran according to any one of claims 1-5, wherein the Ganoderma lucidum is selected from Ganoderma lucidum, Ganoderma sinensis, Ganoderma sinense, and Ganoderma tsugae.
7. The method for preparing ceramide from rice bran by a fermentation method according to claim 1, wherein the prepared ceramide solution and 95% ethanol solution are mixed at a weight ratio of 1:5 at normal temperature, are uniformly stirred and placed overnight, are filtered and precipitated, are concentrated under reduced pressure at 30-40 ℃ under the condition of 0.001-0.01 MPa until the solution is turbid, and are cooled and crystallized under normal pressure to obtain a ceramide solid, wherein the ceramide mass fraction in the ceramide solid is 0.1-1.0%.
8. The method for preparing ceramide from rice bran through fermentation according to claim 1, which comprises the following steps:
raw material treatment: removing impurities from testa oryzae, pulverizing, and sieving;
activating strains: cutting 0.5-1cm from the inclined plane with inoculating hook2 Inoculating the slant Ganoderma mycelia with the culture medium into the middle part of the solid culture medium, and culturing at 28 + -2 deg.C for 3-5 days;
preparing a seed solution: mashing the solid culture medium with Ganoderma with inoculating hook, and adding appropriate amount of sterile water to obtain Ganoderma seed solution;
fermentation culture: mixing the ganoderma lucidum seed liquid and the rice bran raw material powder according to the mass ratio of (1-5) to 1, putting the mixture into a fermentation tank, and controlling the oxygen concentration of the space in the fermentation tank to be 0.010-0.025 mol/L under the condition of 28 +/-2 ℃ for 2-5 days;
homogenizing and breaking walls: after fermentation, adding distilled water with equal weight into the fermented product, mixing, and homogenizing for 1-5 times at 70-120Mpa at normal temperature by high pressure homogenizer to obtain wall-broken homogenate;
removing impurities: removing impurities of the wall-broken homogenate by filtering or centrifuging, and collecting filtrate or supernatant after centrifuging to obtain ceramide solution.
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