CN108203471A - A kind of technology for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment - Google Patents
A kind of technology for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment Download PDFInfo
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- CN108203471A CN108203471A CN201611175854.9A CN201611175854A CN108203471A CN 108203471 A CN108203471 A CN 108203471A CN 201611175854 A CN201611175854 A CN 201611175854A CN 108203471 A CN108203471 A CN 108203471A
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- zymotic fluid
- polysaccharide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
The invention discloses a kind of technologies for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment.Specifically using pholiota nameko, oyster mushroom, mushroom and the similar macro-fungi of other characters as target material, it is added in the ferment of veterinary antibiotics preparation, continues glycolysis in prior fermentation liquid, extract the purpose fungi polysaccharide in glycolysis liquid.It is characterized in that largely extracting mushroom fungi polysaccharide, it is made to be applied to the fields such as food, health products, drug, auxiliary is antiviral, reduce blood glucose, reducing blood lipid, promotes the effect of immunity of organisms.
Description
Technical field
The invention discloses a kind of technologies for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment.Specifically
Using pholiota nameko, oyster mushroom, mushroom and the similar macro-fungi of other characters as target material, it is added to veterinary antibiotics preparation
In ferment, continue glycolysis in prior fermentation liquid, extract the purpose fungi polysaccharide in glycolysis liquid.It is characterized in that largely extract mushroom
Mushroom fungi polysaccharide makes it be applied to the fields such as food, health products, drug, and auxiliary is antiviral, reduces blood glucose, reducing blood lipid, elevator
The effect of body immunity.The invention belongs to food or field of agricultural products processing.
Background technology
Fungi polysaccharide is widely present in the form of teichoic acid, muramic acid or glycosaminoglycan, peptide glycan or proteoglycans
Fungal cell's wall construction.Fungi polysaccharide is generally considered or facts have proved with anticancer adjuvant, reduces ray damage, protection mucous membrane
The effect of tissue.Wherein lentinan, pachymaran etc. have been applied to clinical auxiliary treatment.Fungi polysaccharide is as functional component page
Start to be applied to cosmetic field.The preferred raw material for developing drug and health food both at home and abroad at present is become.For a long time,
Cell wall structure polysaccharide is because chemical constitution is close, physical arrangement is firm, it is difficult to be detached by standard enzymatic and crush method fully degraded.
Macro-fungi mushroom, yeast and domestic fungus have the advantages that wide in variety, yield is high, and fungi polysaccharide can be opened
It is big to send out potentiality.Ferment brewing process using fruits and vegetables as raw material is related to the complexity of a variety of enzymes of various bacteria, mould and its secretion
Interaction, continuous action and secondary action process, under the autolysis of enzyme, bacterium, mould and a small amount of plant cell wall quilt
Depth degradation, cell wall polysaccharide and intracellular exo polysaccharides are fully discharged.Selection ferment making in, early period, add in mushroom or
Other fungies can increase purpose fungi polysaccharide yield.
Using the technology of ferment self-dissolving degradation Mushroom production fungi polysaccharide, fungi polysaccharide can be largely prepared, is food, medicine
Product, health products and cosmetic industry provide raw material.
Invention content
The purpose of the present invention is obtain a kind of technology for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment.
The technology using ferment fermentation and continue ferment autolysis, can largely prepare purpose fungi polysaccharide, be food, drug,
Health products and cosmetic industry provide raw material.
The present invention is achieved by the following technical solutions, and described utilizes ferment self-dissolving degradation Mushroom production fungi polysaccharide
Technology, include the following steps：
A, using water fruits and vegetables as primary raw material, polishing is slurry, obtains virgin pulp liquid, and addition is sugared in virgin pulp liquid, selection addition yeast,
Lactic acid bacteria and aspergillus seal, and carry out several days of preliminary enzymatic hydrolysis and fermentation under the conditions of certain temperature, filter residue, obtain ferment mother hair
Zymotic fluid；
B, mushroom reconciles into slurries after being polished into slurries or dry product polishing, is closed by a certain percentage with the ferment mother zymotic fluid of step A
And select to add in enzyme, sealing continues the regular period of fermenting, filters residue, obtains production zymotic fluid；
C, the polysaccharide in extraction production zymotic fluid, obtains purpose fungi polysaccharide.
Water fruits and vegetables described in step A include apple, pears, peach, cherry, grape, muskmelon, celery, persimmon, radish, south
Melon and other plant products with similar features.
The sugar added in virgin pulp liquid described in step A, its ratio be the 0% ~ 10% of virgin pulp liquid, including glucose, sucrose,
Lactose and sugar alcohol.
Selection described in step A adds in yeast, lactic acid bacteria and aspergillus, and additional proportion is the 0% ~ 5% of virgin pulp liquid, including
Saccharomyces cerevisiae, brewer's yeast, lactobacillus, vinegar milk bacillus, streptococcus thermophilus, monascus, aspergillus oryzae, aspergillus niger and it is other have phase
Like the strain of function.
Several days of preliminary enzymatic hydrolysis and fermentation are carried out under the conditions of certain temperature described in step A, temperature is 50 ~ 60 DEG C,
Number of days is 14 ~ 90 days.
Mushroom described in step B, including pholiota nameko, mushroom, oyster mushroom and other macro-fungis.
Enzyme described in step B, including with protease, cellulase, pectase.
The polysaccharide in extraction production zymotic fluid described in step C, extracting method include alcohol extracting method, liquid phase method, electrophoresis
Method, membrane separation process, acid precipitation method and dialysis.
Specific embodiment
Following embodiment makes it clearer and more definite, but be never used for limiting the scope of the invention for illustrating the present invention.
Embodiment 1
1）Apple is polished slurry, obtain virgin pulp liquid, virgin pulp liquid is packed into glass jar to 2/3 capacity, seals, under the conditions of 15 ~ 25 DEG C
Fermentation 4 ~ 6 weeks, filters residue with double gauze, obtains female zymotic fluid；
2）The pholiota nameko and mushroom of equivalent are taken, is polished into mushroom slurries, mushroom slurries are merged with female zymotic fluid of equal proportion, is filled
Enter glass jar to 3/4 capacity, sealing ferment 3 ~ 6 months under the conditions of 15 ~ 25 DEG C, taking-up, with 1500 rpm of large capacity centrifuge from
The heart takes supernatant, obtains production zymotic fluid；
3）Mixed fungus polysaccharide is obtained using ethanol precipitation, then cross post separation with macroreticular resin to production zymotic fluid, obtains essence
Purpose fungi polysaccharide processed.
Embodiment 2
1）It the apple of equivalent, grape, goes walnut, celery polishing slurry, obtains virgin pulp liquid, add in the beer ferment of virgin pulp liquid 0.1%
Female, vinegar milk bacillus active bacteria formulation and cellulase add in the sucrose of virgin pulp liquid 2%, and loading glass jar to 2/3 capacity seals, 18 ~
It ferments 14 ~ 21 days under the conditions of 25 DEG C, filters residue with double gauze, obtain female zymotic fluid；
2）The pholiota nameko of equivalent, mushroom, oyster mushroom are taken, is polished into mushroom slurries, mushroom slurries with female zymotic fluid according to 1:2 ratio
Example merges, and is packed into glass jar to 3/4 capacity, seals, ferment 3 ~ 6 months under the conditions of 15 ~ 22 DEG C, take out, with large capacity centrifuge
1500 rpm are centrifuged, and take supernatant, obtain production zymotic fluid；
3）Mixed fungus polysaccharide, the desalination of 8000M semi-permeable membranes and monosaccharide, three times chlorine are obtained using ethanol precipitation to production zymotic fluid
Imitative-n-butanol takes off albumen, then crosses post separation with macroreticular resin, obtains refined purpose fungi polysaccharide.
Claims (8)
- A kind of 1. technology for continuing fermentation self-dissolving degradation Mushroom production fungi polysaccharide using ferment, it is characterised in that following steps：A, using water fruits and vegetables as primary raw material, polishing is slurry, obtains virgin pulp liquid, 0% ~ 10% sugar is added in virgin pulp liquid, selection adds Enter yeast, lactic acid bacteria and aspergillus, seal, carry out several days of preliminary enzymatic hydrolysis and fermentation under the conditions of certain temperature, filter residue, obtain ferment Plain mother's zymotic fluid；B, mushroom reconciles into slurries after being polished into slurries or polishing, merges by a certain percentage with the ferment mother zymotic fluid of step A, selects Addition enzyme is selected, is sealed, continues the regular period of fermenting, filters residue, obtains production zymotic fluid；C, the polysaccharide in extraction production zymotic fluid, obtains purpose fungi polysaccharide.
- 2. the water fruits and vegetables according to claim 1A include apple, pears, peach, cherry, grape, muskmelon, celery, persimmon, trailing plants One or more of fore-telling, pumpkin and other plant products with similar features, and the present invention is not limited to the example above ranges.
- 3. the sugar added in the virgin pulp liquid according to claim 1A, its ratio be the 0% ~ 10% of virgin pulp liquid, including glucose, One or more of sucrose, lactose and sugar alcohol, and the present invention is not limited to the example above ranges.
- 4. the selection according to claim 1A adds in yeast, lactic acid bacteria, aspergillus and enzyme, it can add in and also be added without, can add Enter Saccharomyces cerevisiae, brewer's yeast, lactobacillus, vinegar milk bacillus, streptococcus thermophilus, monascus, aspergillus oryzae, aspergillus niger and other have One or more of strain of identity function, and the present invention is not limited to the example above ranges.
- 5. carrying out several days of preliminary enzymatic hydrolysis and fermentation under the conditions of the certain temperature according to claim 1A, temperature is 50 ~ 60 DEG C, number of days is 14 ~ 90 days.
- 6. according to the mushroom described in claim 1B, including one kind in pholiota nameko, mushroom, oyster mushroom and other macro-fungis or It is several, and the present invention is not limited to the example above ranges.
- 7. according to the enzyme described in claim 1B, enzyme preparation additive amount is the 0.01% ~ 0.1% of female zymotic fluid, including protease, One or more of cellulase and pectase, and the present invention is not limited to the example above ranges.
- 8. the polysaccharide in extraction production zymotic fluid according to claim 1C, method include alcohol extracting method, liquid phase method, electrophoresis One or more of method, membrane separation process, acid precipitation method and dialysis, and the present invention is not limited to the example above ranges.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110128563A (en) * | 2019-06-06 | 2019-08-16 | 森田药妆股份有限公司 | A kind of ferment hydrolysis pholiota nameko polysaccharide and its preparation method and application |
| CN110236199A (en) * | 2019-07-10 | 2019-09-17 | 吉林农业大学 | It is a kind of using ferment lentinan as functional component elimination oral peculiar smell preparation |
| CN111718429A (en) * | 2020-07-21 | 2020-09-29 | 暨南大学 | Mushroom edible fungus polysaccharide and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106119055A (en) * | 2016-09-14 | 2016-11-16 | 天津替代医学科技股份有限公司 | A kind of multi-functional ferment vinegar |
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2016
- 2016-12-19 CN CN201611175854.9A patent/CN108203471A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119055A (en) * | 2016-09-14 | 2016-11-16 | 天津替代医学科技股份有限公司 | A kind of multi-functional ferment vinegar |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110128563A (en) * | 2019-06-06 | 2019-08-16 | 森田药妆股份有限公司 | A kind of ferment hydrolysis pholiota nameko polysaccharide and its preparation method and application |
| CN110236199A (en) * | 2019-07-10 | 2019-09-17 | 吉林农业大学 | It is a kind of using ferment lentinan as functional component elimination oral peculiar smell preparation |
| CN111718429A (en) * | 2020-07-21 | 2020-09-29 | 暨南大学 | Mushroom edible fungus polysaccharide and preparation method and application thereof |
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