CN108210556A - The extracting method of active constituent in a kind of diaphragma juglandis - Google Patents
The extracting method of active constituent in a kind of diaphragma juglandis Download PDFInfo
- Publication number
- CN108210556A CN108210556A CN201810211545.5A CN201810211545A CN108210556A CN 108210556 A CN108210556 A CN 108210556A CN 201810211545 A CN201810211545 A CN 201810211545A CN 108210556 A CN108210556 A CN 108210556A
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- CN
- China
- Prior art keywords
- diaphragma juglandis
- active constituent
- extracting method
- extraction
- diaphragma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a kind of extracting methods of active constituent in diaphragma juglandis.It is adopted the technical scheme that：Using diaphragma juglandis as raw material, its active constituent is extracted using Vacuum cavitation, the method has many advantages, such as that extraction efficiency is high, equipment is simple, easily operated, and obtained diaphragma juglandis extract can be used for treating syndrome of deficiency of kidney yang.
Description
Technical field
The present invention relates to a kind of extracting methods of active constituent in diaphragma juglandis, and in particular to is divided using Vacuum cavitation
Active constituent and for treating kidney-yang deficiency in heart wood, belongs to medicinal plant development field.
Background technology
Drying wooden diaphragm of the diaphragma juglandis for juglandaceae plant English walnut (Juglans regia L.) fruit stone, also known as estrangement
Wood, English walnut every.Gas is micro-, bitter and puckery flavor, mild-natured, enters spleen, kidney two warp, nontoxic, and clinic is mainly used for seminal emission lingering diarrhea, gonorrhoea, blood urine, rushes down
Dysentery, ephritis etc. also have effects that canker sore, bleeding gums.A variety of active ingredients is isolated in diaphragma juglandis, such as flavones, soap
Glycosides, alkaloid, polysaccharide and tannin etc..In the prior art, diaphragma juglandis only has soaked steep in wine of a few peoples to drink, and active ingredient obtains not
To abundant extraction, the more poor tastes of impurity should not make health liquor long-term drinking.Therefore, it is necessary to be opened for pharmacy and food health industry
Active constituent in extraction diaphragma juglandis of sending out extracting method a kind of.The method of reactive compound is extracted from traditional plant material as impregnated
Extraction and circumfluence distillation, but will consume a large amount of organic solvents and energy as extraction time extends.
Vacuum cavitation extraction efficiency is high, equipment is simple and easily operated, suitable for industrialized production.In the present invention,
Active constituent in diaphragma juglandis is extracted using Vacuum cavitation and is used to treat kidney-yang deficiency.
Invention content
The purpose of the present invention is to provide a kind of extracting methods of active constituent in diaphragma juglandis.
To achieve the above object, the present invention uses following technical scheme：By diaphragma juglandis by 3-7 days natural air dryings or
12-24h is dried at 30-50 DEG C, it is spare to cross 60 mesh screens after crushed.Diaphragma juglandis dried powder is taken to add in the ethyl alcohol of 40-80%
Solution, solid-liquid ratio 1:10-1:30, negative pressure cavitation extraction pressure is -0.03--0.08MPa, extraction time 20-60min, is extracted
Number 2-4 times merges extracting solution, is concentrated under reduced pressure into dry.
Advantages of the present invention：
(1) it is good to treat kidney-yang deficiency effect using active constituent in Vacuum cavitation extraction diaphragma juglandis by the present invention；
(2) present invention realizes making full use of for diaphragma juglandis resource, and final products yield is high, and yield is more than 75%.
Description of the drawings
(Tu Zhong chinkuei shin chewan pills treatment group is Control to SOD contents, and kidney-yang deficiency model group is in 1 Sample serum of attached drawing
Model, extract high dose group FXM1, extract middle dose group are FXM2, extract low dose group FXM3)
(Tu Zhong chinkuei shin chewan pills treatment group is Control to MDA contents, and kidney-yang deficiency model group is in 2 Sample serum of attached drawing
Model, extract high dose group FXM1, extract middle dose group are FXM2, extract low dose group FXM3)
(Tu Zhong chinkuei shin chewan pills treatment group is Control, kidney yang for cAMP, cGMP and testosterone concentration in 3 Sample serum of attached drawing
Virtual model group is Model, and extract high dose group FXM1, extract middle dose group is FXM2, and extract low dose group is
FXM3)
Specific embodiment
Embodiment 1：
200g diaphragma juglandis dried powder is added in into 50% ethanol solution, with solid-liquid ratio 1:Negative pressure cavitation is carried out after 20 mixing to carry
It takes, under conditions of negative pressure -0.06MPa, extraction time 30min.Extraction time 3 times, merge extracting solution, be concentrated under reduced pressure into it is dry,
Obtain extract, yield 78.3%
Take the male mice 60 of weight 27-33g, be divided into 6 groups (including blank control group, kidney-yang deficiency model group, gold it is deficient
Shenqi pill treatment group, the high, medium and low dosage group of extract), every group of 10 mouse, except blank control group is injected intraperitoneally with 25mg/kg
Outside physiological saline, hydrocortisone, once a day, successive administration 7 days is injected intraperitoneally with the dosage of 25mg/kg in remaining each group.
In addition to blank control group, kidney-yang deficiency model group are with the dosage distilled water gavage of 10mg/kg, chinkuei shin chewan pills treatment group with
The dosage gavage of 0.75mg/kg, the high, medium and low given low of diaphragma juglandis extract for treating group be respectively 0.5mg/kg, 1mg/kg,
2mg/kg.Before daily intraperitoneal injection or gavage, every mouse weight (g) is recorded；Observe the variation of mouse sign.Gavage terminates
Afterwards, blood sampling separation serum, measures SOD, MDA, cAMP, cGMP and testosterone concentration in serum.The experimental results showed that Vacuum cavitation
The diaphragma juglandis extract of acquisition has good treatment kidney-yang deficiency effect.
Claims (4)
1. a kind of extracting method of active constituent in diaphragma juglandis, it is characterised in that：Diaphragma juglandis crushed after being dried, by powder and extraction
Solvent, which adds in, carries out negative pressure cavitation extraction in negative pressure cavitation instrument, merge extracting solution, is concentrated under reduced pressure into do and be extracted to get to diaphragma juglandis
Object.
2. the extracting method of active constituent in a kind of diaphragma juglandis according to claim 1, it is characterised in that：Described diverts one's attention
Wooden crushed after being dried refers to diaphragma juglandis by 3-7 days natural air dryings or 12-24h is dried at 30-50 DEG C, crosses 60 mesh after crushed
Sieve is spare.
3. the extracting method of active constituent in a kind of diaphragma juglandis according to claim 1, it is characterised in that：The extraction
Ethyl alcohol of the solvent for 40-80%, solid-liquid ratio 1:10-1:30.
4. the extracting method of active constituent in a kind of diaphragma juglandis according to claim 1, it is characterised in that：The negative pressure
Cavitation extracts pressure as -0.03--0.08MPa, extraction time 2-4 times, extraction time 20-60min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810211545.5A CN108210556A (en) | 2018-03-15 | 2018-03-15 | The extracting method of active constituent in a kind of diaphragma juglandis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810211545.5A CN108210556A (en) | 2018-03-15 | 2018-03-15 | The extracting method of active constituent in a kind of diaphragma juglandis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108210556A true CN108210556A (en) | 2018-06-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810211545.5A Pending CN108210556A (en) | 2018-03-15 | 2018-03-15 | The extracting method of active constituent in a kind of diaphragma juglandis |
Country Status (1)
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| CN (1) | CN108210556A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902666A (en) * | 2018-07-23 | 2018-11-30 | 大理市大辣小酸食品有限公司 | It is a kind of using Diaphragma juglandis as colorant of raw material and the preparation method and application thereof |
| CN108997509A (en) * | 2018-07-24 | 2018-12-14 | 山东省医学科学院药物研究所（山东省抗衰老研究中心、山东省新技术制药研究所） | A kind of Preparation method and use of wood polysaccharose of diverting one's attention, wood polysaccharose of diverting one's attention |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102093374A (en) * | 2010-11-30 | 2011-06-15 | 东北林业大学 | Method for efficiently extracting camptothecin derivative |
| CN104256011A (en) * | 2014-09-18 | 2015-01-07 | 鲍刚 | Application of diaphragma juglandis fructus to preparation of drinking tea |
| CN107375464A (en) * | 2017-09-08 | 2017-11-24 | 东北林业大学 | The extracting method of active component in a kind of sorbic acid wastewater |
-
2018
- 2018-03-15 CN CN201810211545.5A patent/CN108210556A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102093374A (en) * | 2010-11-30 | 2011-06-15 | 东北林业大学 | Method for efficiently extracting camptothecin derivative |
| CN104256011A (en) * | 2014-09-18 | 2015-01-07 | 鲍刚 | Application of diaphragma juglandis fructus to preparation of drinking tea |
| CN107375464A (en) * | 2017-09-08 | 2017-11-24 | 东北林业大学 | The extracting method of active component in a kind of sorbic acid wastewater |
Non-Patent Citations (2)
| Title |
|---|
| 杨明珠等: "核桃分心木化学成分与生物活性研究 ", 《天然产物研究与开发》 * |
| 王艳: "维吾尔药核桃分心木不同提取物对肾阳虚模型小鼠的实验研究", 《新疆医科大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902666A (en) * | 2018-07-23 | 2018-11-30 | 大理市大辣小酸食品有限公司 | It is a kind of using Diaphragma juglandis as colorant of raw material and the preparation method and application thereof |
| CN108997509A (en) * | 2018-07-24 | 2018-12-14 | 山东省医学科学院药物研究所（山东省抗衰老研究中心、山东省新技术制药研究所） | A kind of Preparation method and use of wood polysaccharose of diverting one's attention, wood polysaccharose of diverting one's attention |
| CN108997509B (en) * | 2018-07-24 | 2021-01-01 | 山东省医学科学院药物研究所（山东省抗衰老研究中心、山东省新技术制药研究所） | Diaphragma juglandis polysaccharide, preparation method and application thereof |
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