CN105385747A - Method for preparing phenolic acid compound through fatsia japonica - Google Patents
Method for preparing phenolic acid compound through fatsia japonica Download PDFInfo
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- CN105385747A CN105385747A CN201510666896.1A CN201510666896A CN105385747A CN 105385747 A CN105385747 A CN 105385747A CN 201510666896 A CN201510666896 A CN 201510666896A CN 105385747 A CN105385747 A CN 105385747A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
Abstract
The invention relates to a method for preparing a phenolic acid compound through fatsia japonica. According to the method, the phenolic acid compound is obtained with fatsia japonica as a raw material through extraction and separation or obtained through biological conversion of aspergillus niger and eurotium after extraction and separation. According to the method, the phenolic acid compound in fatsia japonica is researched and quantitatively analyzed for the first time, and the total amount of the phenolic acid compound in fatsia japonica is 0.279-1.38%. Besides, the yield of a dicaffeoylquinic acid compound is increased through the biological conversion method, and a new raw material and a new method are provided for obtaining the phenolic acid compound.
Description
Technical field
The invention provides a kind of the new raw material source and the preparation method that prepare phenolic acid compound, particularly use aspergillus niger enzyme, loose capsule bacterium enzyme liquid is converted and improves the method for product formation, belongs to the field of bioconversion of natural product.
Background technology
Phenolic acid compound is that a class is extensively present in plant materials and derives by carbohydrate metabolism the class secondary metabolite formed, and has a benzene nucleus, mostly is the derivative of P-hydroxybenzoic acid and p-Coumaric Acid (styracin).Mainly contain hydroxy cinnamate acids, comprise coffic acid, P-coumaric acid and forulic acid etc.; Hydroxy-benzoic acid class, comprises Protocatechuic Acid and gallic acid etc.; Depside, comprise chlorogenic acid and isochlorogenic acid etc. (child, Du Lili, Wang Dong, Liu Xiaoqiu. the progress [J] of Chinese medicine liposoluble ingredient. contemporary Chinese Chinese medicine, 2006,8 (2): 25-28).
Caffeoylquinic acids (Caffeoylquinicacid, CQA) is a class by the coffic acid of quininic acid and different number by the phenolic acids natural component of esterification condensation.Comprise caffeoylquinic acid, (dragon is gentle and quiet for dicaffeoylquinic acid and three caffeoylquinic acids, methyl chlorogenate, Chlorogenic ethyl ester etc., Zhang Sheng, Yuan Ling, Li Yinhua, Liu Zhonghua. RP-HPLC look boils 6 kinds of chlorogenic acid compounds [J] in method Simultaneously test coffee berry. chromatogram, 2011 (5): 439-422).
Chlorogenic acid (Chlorogenicacids, CGA) caffeotannic acid is had another name called, chemistry 3-O-Cafeoylquinic acid (3-O-caffeoylquinicacid) by name, be plant materials a kind of phenylpropanoids through shikimic acid pathway generation in aerobic repiration process, extensively exist with high dicotyledons and pteridophyte.Be soluble in ethanol, methyl alcohol polar solvent, be slightly soluble in ethyl acetate.Chlorogenic acid have the important physiologically active such as antibacterial, antiviral, antitumor, hypotensive (Chen Shaohua, Wang Yaqin, Luo Lixin. the progress [J] of research on chlorogenic acid. food science and technology, 2008 (02): 195-198; Deng Liang, Yuan Hua, analogy ancestor river flowing from Guizhou Province through Hunan into Dongting Lake. the progress [J] of chlorogenic acid. chemistry and biotechnology, 2005 (7): 4-6).
Common dicaffeoylquinic acid (Dicaffeoylquinicacids, Di-CQA) 1 is had, 3-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, Isochlorogenic acid C etc., these compounds generally have hepatic cholagogic effect.From Chinese medicine Inula Flower, be separated the 1,5-Dicaffeoylquinic acid (IBE-5) obtained is a kind of anti-hepatitis B virus of new texture type and the compound of hiv virus, formally enters human clinical trial's stage a few days ago.But 1,5-Dicaffeoylquinic acid content in plant is lower, purifying process is complicated, and be difficult to carry out large-scale production, can only be synthesized obtain by artificial chemistry, its synthetic method obtains Chinese invention patent mandate quininic acid.
1,5-Dicaffeoylquinic acid (1,5-Dicaffeoylquiniccid) molecular structure
Ministry of Health's " drug standard " includes has Chinese patent medicine 170 kinds that is clearing heat and detoxicating, antisepsis and anti-inflammation, all containing chlorogenic acid and be main component.The polyphenols such as chlorogenic acid are called as " the 7th class nutrient substance ", be widely used in healthcare industry, the health care medicine that with the addition of chlorogenic acid has the feature (Deng Liang such as clearing heat and detoxicating, beauty treatment moisturizing, releasing tobacco and wine are too much, Yuan Hua, analogy ancestor river flowing from Guizhou Province through Hunan into Dongting Lake. the progress [J] of chlorogenic acid. chemistry and biotechnology, 2005, (7): 4-6).The phenolic hydroxyl structure of natural phenolic acid compounds has physiologically active widely, as anti-oxidant, scavenging free radicals, anti-ultraviolet radiation, hepatic cholagogic, antibacterial and antivirus action.Be applied in widely food, medicine, daily cosmetics industry (Wujiang great waves. the biological activity of chlorogenic acid and application [J] thereof. modern agriculture science and technology, 2009 (19): 349-350).
Rhizoma et Radix Dysosmae Veitchii (Fatsiajaponica) is Araliaceae (Araliaceae) evergreen shrubs, has another name called eight gold medal dishes, eight hands, hand tree and Euphorbia antiquorum etc., belongs to cloudy raw subtropics adaptive plant.Originate in China Taiwan and Japan, existing Yangtze valley areas to the south extensively distributes.Record according to " Taiwan medicinal plant will ", Rhizoma et Radix Dysosmae Veitchii taste is pungent, bitter, warm in nature, mild toxicity.Its leaf, root, micromicro are used as medicine, can preventing phlegm from forming and stopping coughing, blood-activating analgetic, wound etc.In recent years, medical circle is commonly used Rhizoma et Radix Dysosmae Veitchii and is used as medicine together with the rhizome of other plant, the disease such as Hepatoma therapy, urinary stone disease.Research shows that triterpene saponin componds is the activeconstituents in Rhizoma et Radix Dysosmae Veitchii, also have flavones, volatilization wet goods composition in addition, and the research of phenolic acid compound rarely has report.
2015, Huang Jiandan etc. are extracted the total flavones composition of Rhizoma et Radix Dysosmae Veitchii flower and measure its anti-oxidant activity, find that Rhizoma et Radix Dysosmae Veitchii flower total flavones has DPPH radical scavenging activity and is slightly better than vitamins C (Huang Jiandan, Jiang Yihua. the brightly yellowish ketone optimal extraction technology of Rhizoma et Radix Dysosmae Veitchii and oxidation activity [J] thereof. Chemical Industry in Guangzhou, 2015,43 (4): 64-67).The propagation of Rhizoma et Radix Dysosmae Veitchii branches and leaves extract to human breast cancer cell line Bcap-37, Non-small cell lung carcinoma A549 and human B cell type acute lymphoblastic leukemia cell BALL-1 has certain restraining effect (Zhao Yang, Liang Chao, Li Tuo etc. Rhizoma et Radix Dysosmae Veitchii branches and leaves extract is in the application prepared in cancer therapy drug and preparation [P]. Chinese patent: CN201410352419.3).Lu Zulin research finds to be used as medicine with Rhizoma et Radix Dysosmae Veitchii rhizome, to Hepatoma therapy have certain curative effect (Lu Zulin. the clinical experience [J] of Rhizoma et Radix Dysosmae Veitchii Hepatoma therapy. Zhejiang combination of Chinese tradiational and Western medicine magazine, 1995,19 (2): 13).
There is research and utilization aspergillus niger hydrolysed chlorogenic acid can obtain quininic acid and coffic acid (Lu Dingqiang, Wang Jun, Ling Xiuquan etc. the Aspergillus niger strain of a plant height yield of chlorogenic acid hydrolase and application [P] thereof. Chinese patent: 200810156047.1), obtains coffic acid and quininic acid being worth higher chlorogenic acid decomposition.Research of the present invention finds that aspergillus niger or loose capsule bacterium can transform coffic acid and quininic acid generates dicaffeoylquinic acid, and can obtain the product such as quinic acid, coffic acid.This obtains quininic acid from above-mentioned patent hydrolysed chlorogenic acid and coffic acid is different, and the present invention provides effective means for obtaining dicaffeoylquinic acid.
Aspergillus niger (Aspergillusniger) belongs to Deuteromycotina, hyphomycetes, hyphomycetales, Moniliaceae is a Common Species in Aspergillus, it is important fermentation industry bacterial classification, as long as be citric acid, glyconic acid and gallic acid etc. produce bacterium (Guo Yanmei, Zheng Ping, Sun Jibin. aspergillus niger is as cell factory: knowledge prepare with technical foundation [J]. biotechnology journal, 2010,26 (10): 1410-1418).Eurotium Ascomycota, loose capsule Gammaproteobacteria, Eurotiale, amylase, lipase, proteolytic enzyme etc. can be produced, the present invention loose capsule bacterium used is of the present invention group and once used bacterial strain, comprise coronoid process fall apart capsule bacterium (Eurotiumcristatum), thorn spore fall apart capsule bacterium (Eurotiumechinulatum), thank to Wa Shi and to fall apart capsule bacterium (Eurotiumchevalieri) etc.(Chen Yuru, Sun Huan, Qian Yuanyuan etc. loose capsule bacterium utilizes inner ether sugar and Mierocrystalline cellulose pyrolytic behavior etc. to produce melanic method [P]. Chinese patent: 201310131501.9).
At present, have no the research of phenolic acid compound in Rhizoma et Radix Dysosmae Veitchii is reported.
Summary of the invention
The present invention utilizes Araliaceae Rhizoma et Radix Dysosmae Veitchii to extract phenolic acid compound, and utilizes bioconversion method to improve the output of di-coffee mesitoyl quinine acid compounds, adds the approach obtaining phenolic acid compound.
The present invention explores the phenolic acid compound in Rhizoma et Radix Dysosmae Veitchii first, result shows that the phenolic acid compound kind in Rhizoma et Radix Dysosmae Veitchii is more, there are coffic acid, quinic acid, chlorogenic acid and 3,4 dicaffeoylquinic acid (as shown in Figure 3), 3,5 dicaffeoylquinic acid (as shown in Figure 4), 4,5 dicaffeoylquinic acid (as shown in Figure 5)) etc.In Rhizoma et Radix Dysosmae Veitchii, phenolic acid compound content is shown in Table 1, and in Rhizoma et Radix Dysosmae Veitchii, phenolic acid compound content is stem skin, tender leaf, fruit, climax leaves, Lao Ye from high to low successively, and wherein the proportion that accounts for of dicaffeoylquinic acid is the highest.Can also infer that phenolic acid compound has downward trend gradually in the blade ripening process of Rhizoma et Radix Dysosmae Veitchii according to the content of phenolic acid compound simultaneously.The comprehensive utilization of Rhizoma et Radix Dysosmae Veitchii of the present invention provides foundation, provides new raw material type for obtaining phenolic acid compound.
Table 1 Rhizoma et Radix Dysosmae Veitchii different sites phenolic acid compound content (mgg
-1)
The invention provides a kind of method utilizing new resources Araliaceae Rhizoma et Radix Dysosmae Veitchii to prepare phenolic acid compound.Chlorogenic acid, coffic acid, quinic acid and dicaffeoylquinic acid (Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C) can be obtained.
The method of phenolic acid compound is prepared in a kind of Rhizoma et Radix Dysosmae Veitchii; Be take Rhizoma et Radix Dysosmae Veitchii as raw material, carry out extraction and isolation; Or obtain phenolic acid compound through aspergillus niger, loose capsule bacterium bio-transformation again after extraction and isolation.
Extract the method for phenolic acid compound with Araliaceae Rhizoma et Radix Dysosmae Veitchii raw material, it is characterized in that, described method comprises:
Described extraction operation comprises any one in lixiviate, ultrasonic extraction, refluxing extraction, microwave extraction extraction;
Described conversion operation comprises any one in conversion;
Described purification process comprises one or more in high speed centrifugation purifying, resin purification, organic extractant solution, ultrafiltration purification;
Described pending Rhizoma et Radix Dysosmae Veitchii is specially the fruit of Araliaceae Rhizoma et Radix Dysosmae Veitchii, leaf, stem, root;
Described Extraction solvent comprises: the aqueous solution or volume fraction are ethanol, the methyl alcohol of 10%-100%; PH is 3.0-7.0.
Method of the present invention aspergillus niger or loose capsule bacterium is produced enzyme liquid to add Rhizoma et Radix Dysosmae Veitchii herb extracting solution and carry out bio-transformation.
Described conversion operation comprises with Rhizoma et Radix Dysosmae Veitchii stem, leaf and fruit for raw material, the method that Rhizoma et Radix Dysosmae Veitchii herb extracting solution improves dicaffeoylquinic acid content is transformed by enzyme liquid, the described extracting solution containing phenolic acid compound is after transforming 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4, the content of 5-dicaffeoylquinic acid increases by 969% respectively, and 277%, 253%.
Aspergillus niger of the present invention and loose capsule bacterium can be screened from nature, also can from CGMCC CICC CFCC the Spawn preservation organization such as ATCC buy.
In aforesaid method, invert point 30 DEG C-90 DEG C (optimum temps is 45 DEG C), transformation system pH is 3.0-9.0 (Optimal pH is 5.0), transformation time 0.1h-48h (the optimal conversion time is 24h).
In aforesaid method, conversion of substrate is Rhizoma et Radix Dysosmae Veitchii extracting solution, wherein the concentration 1% ~ 50% (optimum concn 5%) of phenolic acid compound.
The method purifying such as the post separation that the product in conversion fluid of the present invention is conventional.
Advantage of the present invention:
1. phenolic acid compound rich content in Araliaceae Rhizoma et Radix Dysosmae Veitchii, wherein the proportion that accounts for of dicaffeoylquinic acid is the highest, and the total amount of phenolic acid compound is between 0.279% ~ 1.38%.
2. the biomass of Araliaceae Rhizoma et Radix Dysosmae Veitchii raw material is large, and cultivated area is wide, and in the present invention, the application of the microorganism such as aspergillus niger and loose capsule bacterium provides the method being obtained more particular phenol acid compounds by bio-transformation, has broad application prospects.
Accompanying drawing explanation
Fig. 1 Rhizoma et Radix Dysosmae Veitchii fruit extracting solution HPLC schemes (note: 1-coffic acid; 2-quinic acid; 3-3,4 dicaffeoylquinic acid; 4-3,5 dicaffeoylquinic acid; 5-4,5 dicaffeoylquinic acid).
HPLC figure (24h) after the bio-transformation of Fig. 2 extracting solution.(note: 1-coffic acid; 2-quinic acid; 3-3,4 dicaffeoylquinic acid; 4-3,5 dicaffeoylquinic acid; 5-4,5 dicaffeoylquinic acid).
Isochlorogenic acid B second order ms figure in Fig. 3 extracting solution.
3,5-diCQA second order ms figure in Fig. 4 extracting solution.
Isochlorogenic acid C second order ms figure in Fig. 5 extracting solution.
Embodiment
Carry out clear, complete description to the technical scheme in the embodiment of the present invention below, described embodiment is only the present invention's part embodiment, instead of whole embodiments, and in embodiment, bacterial classification used is conventional aspergillus niger, loose capsule bacterium.
Embodiment 1:
Rhizoma et Radix Dysosmae Veitchii fruit 500g pulverized 60 mesh sieves, added the ethanolic soln that volume fraction is 70%, supersound extraction by solid-liquid ratio 1:20, and rotary evaporation is extremely without ethanol.Extracting solution is extracted with ethyl acetate, and obtains the extraction liquid containing Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C, quinic acid and the raffinate containing chlorogenic acid, coffic acid, quininic acid.3 of purity 85% is obtained through the further separation and purification of column chromatography after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.28g, 3 of purity 92%, 5-dicaffeoylquinic acid 2.9g, 4 of purity 90%, 5-dicaffeoylquinic acid 0.23g, raffinate can also obtain the product such as coffic acid, quinic acid.
Embodiment 2:
Identical with embodiment 1, be that the ethanolic soln of 70% replaces with the methanol solution that volume fraction is 70% by volume fraction.
Embodiment 3:
Rhizoma et Radix Dysosmae Veitchii fruit 500g pulverized 60 mesh sieves, added by solid-liquid ratio 1:20 the ethanolic soln that volume fraction is 70%, supersound extraction 1 hour, and once, united extraction liquid, extracting solution rotary evaporation is extremely without ethanol in repetitive operation.Extracting solution ethyl acetate equal proportion extraction, obtains the extraction liquid containing Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C, quinic acid and the raffinate containing chlorogenic acid, coffic acid, quininic acid.3 of purity 90% is obtained through the further separation and purification of AB-8 macroporous resin after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.31g, 3 of purity 96%, 5-dicaffeoylquinic acid 2.85g, 4 of purity 90%, 5-dicaffeoylquinic acid 0.37g, raffinate can also obtain the product such as coffic acid, quinic acid.
Embodiment 4:
Identical with embodiment 2, be that the ethanolic soln of 70% replaces with the methanol solution that volume fraction is 70% by volume fraction.
Embodiment 5:
Get after Rhizoma et Radix Dysosmae Veitchii tender leaf 500g dries and pulverized 60 mesh sieves.Add by solid-liquid ratio 1:20 the ethanolic soln that volume fraction is 70%, supersound extraction 1 hour, once, united extraction liquid, extracting solution rotary evaporation is extremely without ethanol in repetitive operation.Extracting solution ethyl acetate equal proportion extraction, obtains the extraction liquid containing Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C, quinic acid and the raffinate containing chlorogenic acid, coffic acid, quininic acid.3 of purity 92% is obtained through the further separation and purification of column chromatography after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.25g, 3 of purity 96%, 5-dicaffeoylquinic acid 3.1g, 4 of purity 90%, 5-dicaffeoylquinic acid 0.29g, raffinate can also obtain the product such as coffic acid, quinic acid.
Embodiment 6:
Get after Rhizoma et Radix Dysosmae Veitchii climax leaves 500g dries and pulverized 60 mesh sieves.Add by solid-liquid ratio 1:20 the ethanolic soln that volume fraction is 70%, supersound extraction 1 hour, once, united extraction liquid, extracting solution rotary evaporation is extremely without ethanol in repetitive operation.Extracting solution ethyl acetate equal proportion extraction, obtains the extraction liquid containing Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C, quinic acid and the raffinate containing chlorogenic acid, coffic acid, quininic acid.3 of purity 90% is obtained through the further separation and purification of column chromatography after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.14g, 3 of purity 92%, 5-dicaffeoylquinic acid 2.3g, 4 of purity 90%, 5-dicaffeoylquinic acid 0.24g, raffinate can also obtain the product such as coffic acid, quinic acid.
Embodiment 7:
Get after Rhizoma et Radix Dysosmae Veitchii Lao Ye 500g dries and pulverized 60 mesh sieves.Add by solid-liquid ratio 1:20 the ethanolic soln that volume fraction is 70%, supersound extraction 1 hour, once, united extraction liquid, extracting solution rotary evaporation is extremely without ethanol in repetitive operation.Extracting solution ethyl acetate equal proportion extraction, obtains the extraction liquid containing Isochlorogenic acid B, 3,5-diCQA, Isochlorogenic acid C, quinic acid and the raffinate containing chlorogenic acid, coffic acid, quininic acid.3 of purity 90% is obtained through the further separation and purification of column chromatography after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.12g, 3 of purity 93%, 5-dicaffeoylquinic acid 2.1g, 4 of purity 90%, 5-dicaffeoylquinic acid 0.22g, raffinate can also obtain the products such as chlorogenic acid, coffic acid, quinic acid.
Embodiment 8:
Get after Rhizoma et Radix Dysosmae Veitchii fruit 500g dries and pulverized 60 mesh sieves.Add by solid-liquid ratio 1:20 the ethanolic soln that volume fraction is 70%, supersound extraction 1 hour, once, united extraction liquid, extracting solution rotary evaporation is extremely without ethanol in repetitive operation.With the aspergillus niger solid koji substratum of routine, aspergillus niger enzyme liquid is obtained through adding flooding after fermentation, the enzyme liquid of 100mL adds the Rhizoma et Radix Dysosmae Veitchii fruit extracting solution that 100mL contains 10% (w/v) phenolic acid compound, and transformation system pH transforms 24h in 5.0,45 DEG C of water-baths.Conversion fluid ethyl acetate equal proportion extracts, 3 of purity 90% is obtained through the further separation and purification of column chromatography after the organic solvent removing of extraction liquid, 4-dicaffeoylquinic acid 0.23g, 3 of purity 95%, 5-dicaffeoylquinic acid 3.7g, the Isochlorogenic acid C 0.37g of purity 93%, raffinate can also obtain the products such as chlorogenic acid, coffic acid, quinic acid.
Embodiment 9:
Identical with embodiment 8, Rhizoma et Radix Dysosmae Veitchii fruit is replaced with Rhizoma et Radix Dysosmae Veitchii tender leaf.
Embodiment 10:
Identical with embodiment 8, Rhizoma et Radix Dysosmae Veitchii fruit is replaced with Rhizoma et Radix Dysosmae Veitchii climax leaves.
Embodiment 11:
Identical with embodiment 8, Rhizoma et Radix Dysosmae Veitchii fruit is replaced with Rhizoma et Radix Dysosmae Veitchii Lao Ye.
Embodiment 12:
Identical with embodiment 8, be that the ethanolic soln of 70% replaces with the methanol solution that volume fraction is 70% by volume fraction.
Embodiment 13:
Identical with embodiment 8, aspergillus niger is replaced with coronoid process and to fall apart capsule bacterium.
Embodiment 14:
Identical with embodiment 8,45 DEG C of water-baths are replaced with 30 DEG C.
Embodiment 15:
Identical with embodiment 8,45 DEG C of water-baths are replaced with 90 DEG C.
Embodiment 16:
Identical with embodiment 8,0.1h will be replaced with switching time.
Embodiment 17:
Identical with embodiment 8,48h will be replaced with switching time.
Embodiment 18:
Identical with embodiment 8, converting system pH is replaced with 3.
Embodiment 19:
Identical with embodiment 8, converting system pH is replaced with 9.
Claims (5)
1. prepare the method for phenolic acid compound with Rhizoma et Radix Dysosmae Veitchii for one kind; It is characterized in that, take Rhizoma et Radix Dysosmae Veitchii as raw material, carry out extraction and isolation; Or obtain phenolic acid compound through aspergillus niger, loose capsule bacterium bio-transformation again after extraction and isolation.
2. method according to claim 1, is characterized in that, with Rhizoma et Radix Dysosmae Veitchii herb for raw material carries out extraction and isolation, the Extraction solvent extracting herb comprises: the aqueous solution or volume fraction are ethanol, the methyl alcohol of 10% ~ 100%.
3. method according to claim 2, is characterized in that, the pH of described Extraction solvent is 3.0-7.0.
4. method according to claim 1, is characterized in that, with Rhizoma et Radix Dysosmae Veitchii herb for raw material, with aspergillus niger, loose capsule bacterium for microbial, obtains enzyme liquid transform associated products through solid or liquid fermenting.
5. method according to claim 4, is characterized in that, invert point 30 DEG C-90 DEG C, and transformation system pH is 3.0-9.0, transformation time 0.1h-48h.
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| CN105755050A (en) * | 2016-05-06 | 2016-07-13 | 华中农业大学 | Method for biologically synthesizing nano silver through fatsia japonica leaf extract |
| CN106508966A (en) * | 2016-10-13 | 2017-03-22 | 常州亚环环保科技有限公司 | Method for preparing slow-release biomass algistat |
| CN108524336A (en) * | 2018-06-28 | 2018-09-14 | 浙江树人学院 | A kind of Fatsia japonica natural activity face cream and preparation method thereof |
| CN110093279A (en) * | 2018-03-21 | 2019-08-06 | 株式会社考莱 | The manufacturing method of new aspergillus niger A-T1 bacterial strain and the natural antimicrobial substance with this |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105755050A (en) * | 2016-05-06 | 2016-07-13 | 华中农业大学 | Method for biologically synthesizing nano silver through fatsia japonica leaf extract |
| CN105755050B (en) * | 2016-05-06 | 2021-09-03 | 华中农业大学 | Method for biologically synthesizing nano silver by using illicium verum leaf extract |
| CN106508966A (en) * | 2016-10-13 | 2017-03-22 | 常州亚环环保科技有限公司 | Method for preparing slow-release biomass algistat |
| CN110093279A (en) * | 2018-03-21 | 2019-08-06 | 株式会社考莱 | The manufacturing method of new aspergillus niger A-T1 bacterial strain and the natural antimicrobial substance with this |
| CN108524336A (en) * | 2018-06-28 | 2018-09-14 | 浙江树人学院 | A kind of Fatsia japonica natural activity face cream and preparation method thereof |
| CN108524336B (en) * | 2018-06-28 | 2021-05-11 | 浙江树人学院 | Natural active radix et rhizoma Dysosmatis repens skin cream and preparation method thereof |
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