CN105087404A - Extruding granulation fermentation method of yeast culture prepared from brewer's grains and related yeast culture particles - Google Patents
Extruding granulation fermentation method of yeast culture prepared from brewer's grains and related yeast culture particles Download PDFInfo
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Abstract
The invention provides an extruding granulation fermentation method of a yeast culture prepared from brewer's grains, wherein yeast culture particles are prepared by the following steps: inoculating saccharomyces cerevisiae to brewer's grains; adding protease, cellulase and a carbon source; fermenting and enzymatically hydrolyzing; and pelleting and drying so as to obtain yeast culture particles. Preferably, before inoculating, water content of the brewer's grains is regulated to 35-60wt% and the content of crude protein is regulated to 18-35wt%. The saccharomyces cerevisiae is prepared by activating alcohol-fermentation active dry yeast. Fermentation temperature is 20-50 DEG C and fermentation duration is 12-36h. Enzymolysis temperature is 35-65 DEG C and enzymolysis duration is 12-60h. The process of pelleting is carried out in a mode of screw extrusion. Drying temperature is 45-75 DEG C and drying duration is 12-30h. The invention also provides related yeast culture particles. The extruding granulation fermentation method of the yeast culture prepared from brewer's grains disclosed by the invention achieves the full utilization of the brewer's grains, so as to reduce environmental pollution; and the fermentation method is simple in operation, low in cost, applicable to mass production and suitable for popularization and application in a large scale.
Description
Technical field
The present invention relates to a kind of is raw material with brewer's grains, through the method for the explained hereafter food yeast culture that fermentation and enzymolysis are worked in coordination with, belongs to technical field of bioengineering.
Specifically refer to that a kind of take brewer's grains as yeast culture extruder grain fermentation process and the related yeasts culture particle of raw material.
Background technology
Yeast culture is a kind of probiotics, mainly adopts yeast saccharomyces cerevisiae, adopts liquid, solid-stately combine or directly after solid medium top fermentation, process obtained product together with solid substrate under modern zymotechnique controls.This product Main Function composition is extracellular metabolic product, the solid substrate fermenting rear sex change, yeast cell contents and the yeast cells wall that yeast fermentation solid substrate produces.Product composition is complicated, containing mineral substance, vitamin B group, increasing taste material, digestive ferment, mannooligo saccharide, beta-glucan and amino acid and " unknown somatomedin " etc., these materials are perfect nutritional substrate for the microorganism in animal gastrointestinal tract, can microbial growth be promoted, and then improve the production performance of animal.It is the microbial forage additive integrating the multi-efficiencies such as health care and nutrition.
Yeast culture can provide nutritive substance to microorganism in animal digestive tract, thus excites micro-ecological environment in their metabolic activity and liptinite.Compared with antibiotics additive, there is green natural, have no side effect and good palatability, the advantage such as nutritious.
Function and the mechanism thereof of yeast culture are mainly:
(1) improve gastrointestinal bacterial flora to fall structure, improve breeding performonce fo animals;
(2) supplement the nutrients, improve digestive function;
(3) immunity of organisms and disease resistance is improved.
China produces brewer's grains per year more than 1,000 ten thousand tons, and because brewer's grains moisture content is large, protein content is high, thus transport, long storage periods and feeding on all there is larger problem.Current brewer's grains only have small part to be used on ruminating animal, and major part does not all well utilize, and pile up corrupt, cause environmental pollution.
Therefore need to provide a kind of yeast culture preparation method, it can make full use of brewer's grains, thus reduces environmental pollution.
Summary of the invention
In order to overcome above-mentioned shortcoming of the prior art, it take brewer's grains as the yeast culture extruder grain fermentation process of raw material that one object of the present invention is to provide a kind of, and it can make full use of brewer's grains, thus reduces environmental pollution, is suitable for large-scale promotion application.
Another object of the present invention is to provide a kind of take brewer's grains as the yeast culture extruder grain fermentation process of raw material, and it is simple to operate, and cost is low, can be mass-produced, and is suitable for large-scale promotion application.
Another object of the present invention is to provide a kind of yeast culture composition granule, it be applicable to various animal edible, easily absorb, easy to digest, nutritious and balance, be suitable for large-scale promotion application.
For reaching above object, in a first aspect of the present invention, thering is provided a kind of take brewer's grains as the yeast culture extruder grain fermentation process of raw material, be characterized in, yeast saccharomyces cerevisiae is inoculated in described brewer's grains, and add proteolytic enzyme, cellulase and carbon source, and through fermentation and enzymolysis, then granulation dry thus make yeast culture composition granule.
In described brewer's grains, water content is generally 76.4 ~ 82.5%, and crude protein content is generally 22.1 ~ 25.7%, and crude fiber content is generally 12.7 ~ 16.2%.
In order to obtain fermentation and hydrolysis result preferably, preferably, before described inoculation, the water content of described brewer's grains being adjusted to 35 ~ 60% weight, the crude protein content of described brewer's grains is adjusted to 18 ~ 35% weight.
Described adjustment can be adopted in any suitable manner, and preferably, described adjustment regulates material to realize by adding.
Described adjustment material can be any suitable material, and preferably, described adjustment material is selected from one or more of wheat bran and dregs of beans.
Described carbon source can be the carbon source of any suitable fermentation by saccharomyces cerevisiae, and preferably, described carbon source is molasses.
Described yeast saccharomyces cerevisiae can adopt any suitable yeast saccharomyces cerevisiae, and preferably, described yeast saccharomyces cerevisiae is activated by Active Dry Yeast and forms.
Described activation can adopt any suitable method, preferably, described activation be described Active Dry Yeast is added 30 ~ 40 DEG C containing sucrose 1 ~ 8% weight warm water in, stir evenly activation 20 ~ 40 minutes.
In order to obtain good activation effect, preferably, the quality of described warm water is 20 ~ 30 times of the quality of described Active Dry Yeast.
The inoculum size of described Active Dry Yeast, the addition of described proteolytic enzyme, the addition of described cellulase, the addition of described carbon source can be determined as required, preferably, the inoculum size of described Active Dry Yeast is every gram of described brewer's grains siccative inoculation 2 ~ 5 × 10
7individual/g siccative; The addition of described proteolytic enzyme is the protein interpolation 100 ~ 500u in every gram of described brewer's grains; The addition of described cellulase is the robust fibre interpolation 150 ~ 400u in every gram of described brewer's grains; The addition of described carbon source is the 1-3% weight of described brewer's grains.
Described fermentation can adopt any suitable condition to carry out, and preferably, the temperature of described fermentation is 20 DEG C ~ 50 DEG C, and the time of described fermentation is 12 ~ 36 hours.
Brewer's grains, the yeast activity dry yeast activated, proteolytic enzyme, cellulase, carbon source can be fermented after mixing.
Described enzymolysis can adopt any suitable condition to carry out, and preferably, the temperature of described enzymolysis is 35 ~ 65 DEG C, and the time of described enzymolysis is 12 ~ 60 hours.
The temperature of enzymolysis can be reached after fermentation by piling up the mode heated up.
According to above preparation method, after fermentation, yeast viable count>=10 × 10
8cfu/g siccative; After enzymolysis, little peptide content>=10% weight, yeast sporoderm-broken rate>=75%, crude fiber content is 6.1 ~ 8.5%.
Described granulation can be adopted and be carried out in any suitable manner, and preferably, described granulation adopts screw extruding mode to carry out.
The particle diameter of described granulation can be determined as required, and preferably, the particle diameter of described granulation is 3mm-7mm.
Described drying can adopt any suitable condition to carry out, and preferably, the temperature of described drying is 45 ~ 75 DEG C, and the time of described drying is 12 ~ 30 hours.
In a second aspect of the present invention, provide a kind of yeast culture composition granule, be characterized in, adopting above-mentioned is that the yeast culture extruder grain fermentation process of raw material is prepared from brewer's grains.
Beneficial effect of the present invention is mainly:
1, of the present invention take brewer's grains as the yeast culture extruder grain fermentation process of raw material is in brewer's grains, inoculate yeast saccharomyces cerevisiae, and add proteolytic enzyme, cellulase and carbon source, through fermentation and enzymolysis, then granulation dry thus make yeast culture composition granule, therefore, it is possible to make full use of brewer's grains, thus minimizing environmental pollution, be suitable for large-scale promotion application.
2, of the present invention take brewer's grains as the yeast culture extruder grain fermentation process of raw material is in brewer's grains, inoculate yeast saccharomyces cerevisiae, and add proteolytic enzyme, cellulase and carbon source, through fermentation and enzymolysis, then granulation dry thus make yeast culture composition granule, therefore simple to operate, cost is low, can be mass-produced, and is suitable for large-scale promotion application.
3, yeast culture composition granule of the present invention inoculates yeast saccharomyces cerevisiae in brewer's grains, and add proteolytic enzyme, cellulase and carbon source, through fermentation and enzymolysis, then granulation dry thus make, be applicable to various animal edible, easily absorb, easy to digest, nutritious and balance, be suitable for large-scale promotion application.
These and other objects of the present invention, characteristics and advantages, fully demonstrated by following detailed description and claim, and be achieved by means, device and their combination specially pointed out in claims.
Embodiment
The present invention take brewer's grains as raw material, and inoculation yeast saccharomyces cerevisiae, adds zymin, adopts the collaborative technique of fermentation enzymolysis to prepare yeast culture.The present invention generally includes the following step:
1, prepare burden: interpolation wheat bran, dregs of beans etc. regulate material thus the water content of adjustment brewer's grains is 35 ~ 60%, and crude protein content is 18 ~ 35%, and crude fiber content is 8 ~ 12%.
2, actication of culture: by Active Dry Yeast, add 30-40 DEG C of 20-30 times of quality containing sucrose 1-8% (massfraction) warm water in, stir evenly activation 20-40 minute.
3, inoculation mixing: the Active Dry Yeast activated, proteolytic enzyme, cellulase, carbon source are mixed with above-mentioned batching, such as, mixing machine can be utilized to mix.The inoculum size of Active Dry Yeast is every gram of described brewer's grains siccative inoculation 2 ~ 5 × 10
7individual; The addition of proteolytic enzyme is the protein interpolation 100 ~ 500u in every gram of brewer's grains; The addition of cellulase is the robust fibre interpolation 150 ~ 400u in every gram of brewer's grains; The addition of carbon source is the 1-3% weight of brewer's grains.
4, temperature controlled fermentation: control temperature is 20 DEG C ~ 50 DEG C, ferments 12 ~ 36 hours, keep air unobstructed, period stirring once.
5, enzymolysis broken wall: continue after stirring to pile up to heat up, control temperature 35 ~ 65 DEG C of enzymolysis 12 ~ 60 hours.
6, granulation: adopt screw extruding mode fermentation materials to be carried out granulation, grain diameter 3-7mm.
7, dry: control temperature is 45 ~ 75 DEG C dries 12 ~ 30 hours.Final water content 10 ~ 12% weight.
8, product aftertreatment, by qualified for the examination and test of products rear packaging, obtains finished product.
Testing index and packaging meet the regulation of company standard.
In order to more clearly understand technology contents of the present invention, describe in detail especially exemplified by following examples.
Embodiment 1:
Take brewer's grains as a yeast culture extruder grain zymotechnique for raw material, its technological process is as follows:
(1) prepare burden: get brewer's grains 1500kg and add wheat bran 100kg, dregs of beans 400kg, make the water content mass percentage content of this material be 60%, protein mass degree is 35%, and crude fiber content is 8%;
(2) actication of culture: (specification is living bacteria count 2 × 10 by Active Dry Yeast
10individual/g) 2kg, add in 60kg syrup the active dry yeast stirring evenly activation and must activate for 40 minutes, syrup temperature is 40 DEG C, and in syrup, the mass percentage content of sucrose is 8%;
(3) inoculation mixing: gained material in the active dry yeast activated, proteolytic enzyme (specification is 50000u/g) 7kg, cellulase (specification is 50000u/g) 1.28kg, molasses 60kg and step (1) is mixed;
(4) temperature controlled fermentation: control temperature is 50 DEG C, ferments 12 hours, period stirring once, keep air unobstructed, wherein containing yeast viable count 12.5 × 10
8cfu/g siccative;
(5) enzymolysis broken wall: continue to pile up and heat up, control temperature is 65 DEG C, enzymolysis 12 hours, its medium and small peptide content 13.2% weight, yeast sporoderm-broken rate 91.7%, and crude fiber content is 6.1%;
(6) granulation: adopt screw extruding mode that fermentation materials is carried out granulation and obtain yeast culture composition granule, yeast culture grain diameter 7mm;
(7) dry: be 75 DEG C by yeast culture Grain size controlling temperature, drying 12 hours must product, and now the moisture mass percent of product is 10.8%;
(8) product aftertreatment: pack after the examination and test of products, obtains finished product.
Embodiment 2:
Take brewer's grains as a yeast culture extruder grain zymotechnique for raw material, its technological process is as follows:
(1) prepare burden: get brewer's grains 1700kg and add wheat bran 100kg, dregs of beans 150kg, make the water content mass percent of this material be 48%, protein mass degree 28%, crude fiber content is 10%;
(2) actication of culture: (specification is living bacteria count 2 × 10 by Active Dry Yeast
10individual/g) 2kg, add in 45kg syrup the active dry yeast stirring evenly activation and must activate for 30 minutes, syrup temperature is 35 DEG C, and in syrup, the mass percentage content of sucrose is 4%;
(3) inoculation mixing: gained material in the active dry yeast activated, proteolytic enzyme (specification is 50000u/g) 2.1kg, cellulase (specification is 50000u/g) 0.7kg, molasses 39kg and step (1) is mixed;
(4) temperature controlled fermentation: control temperature is 35 DEG C, ferments 24 hours, period stirring once, keep air unobstructed, wherein containing yeast viable count 11.7 × 10
8cfu/g siccative;
(5) enzymolysis broken wall: continue to pile up and heat up, control temperature is 50 DEG C, enzymolysis 36 hours, its medium and small peptide content 12.7% weight, yeast sporoderm-broken rate 89.8%, and crude fiber content is 7.3%;
(6) granulation: adopt screw extruding mode that fermentation materials is carried out granulation and obtain yeast culture composition granule, yeast culture grain diameter 3mm;
(7) dry: be 60 DEG C by yeast culture Grain size controlling temperature, drying 21 hours must product, and now the moisture mass percent of product is 11.5%;
(8) product aftertreatment: pack after the examination and test of products, obtains finished product.
Embodiment 3:
Take brewer's grains as a yeast culture extruder grain zymotechnique for raw material, its technological process is as follows:
(1) prepare burden: get brewer's grains 2000kg and add wheat bran 400kg, dregs of beans 100kg, make the water content mass percent of this material be 35%, protein mass degree 18%, crude fiber content is 12%;
(2) actication of culture: (specification is living bacteria count 2 × 10 by Active Dry Yeast
10individual/g) 1.625kg, add in 32.5kg syrup the active dry yeast stirring evenly activation and must activate for 20 minutes, syrup temperature is 30 DEG C, and in syrup, the mass percentage content of sucrose is 1%;
(3) inoculation mixing: gained material in the active dry yeast activated, proteolytic enzyme (specification is 50000u/g) 0.9kg, cellulase (specification is 50000u/g) 0.9kg, molasses 25kg and step (1) is mixed;
(4) temperature controlled fermentation: control temperature is 20 DEG C, ferments 36 hours, period stirring once, keep air unobstructed, wherein containing yeast viable count 13.0 × 10
8cfu/g siccative;
(5) enzymolysis broken wall: continue to pile up and heat up, control temperature is 35 DEG C, enzymolysis 60 hours, its medium and small peptide content 10% weight, yeast sporoderm-broken rate 75%, and crude fiber content is 8.5%;
(6) granulation: adopt screw extruding mode that fermentation materials is carried out granulation and obtain yeast culture composition granule, yeast culture grain diameter 5.5mm;
(7) dry: be 45 DEG C by yeast culture Grain size controlling temperature, drying 30 hours must product, and now the moisture mass percent of product is 10.8%;
(8) product aftertreatment: pack after the examination and test of products, obtains finished product.
Embodiment 4:
Take brewer's grains as a yeast culture extruder grain zymotechnique for raw material, its technological process is as follows:
(1) prepare burden: get brewer's grains 1600kg and add wheat bran 100kg, dregs of beans 250kg, make the water content mass percent of this material be 54%, protein mass degree 32%, crude fiber content is 9%;
(2) actication of culture: (specification is living bacteria count 2 × 10 by Active Dry Yeast
10individual/g) 1.35kg, add in 35kg syrup the active dry yeast stirring evenly activation and must activate for 35 minutes, syrup temperature is 37 DEG C, and in syrup, the mass percentage content of sucrose is 4%;
(3) inoculation mixing: gained material in the active dry yeast activated, proteolytic enzyme (specification is 50000u/g) 4.5kg, cellulase (specification is 50000u/g) 1kg, molasses 34kg and step (1) is mixed;
(4) temperature controlled fermentation: control temperature is 40 DEG C, ferments 20 hours, period stirring once, keep air unobstructed, wherein containing yeast viable count 12.8 × 10
8cfu/g siccative;
(5) enzymolysis broken wall: continue to pile up and heat up, control temperature is 58 DEG C, enzymolysis 28 hours, its medium and small peptide content 11.9% weight, yeast sporoderm-broken rate 91.2%, and crude fiber content is 7.2%;
(6) granulation: adopt screw extruding mode that fermentation materials is carried out granulation and obtain yeast culture composition granule, yeast culture grain diameter 5.6mm;
(7) dry: be 69 DEG C by yeast culture Grain size controlling temperature, drying 19 hours must product, and now the moisture mass percent of product is 10.9%;
(8) product aftertreatment: pack after the examination and test of products, obtains finished product.
Embodiment 5:
Take brewer's grains as a yeast culture extruder grain zymotechnique for raw material, its technological process is as follows:
(1) prepare burden: get brewer's grains 1900kg and add wheat bran 100kg, dregs of beans 100kg, make the water content mass percent of this material be 43%, protein mass degree 25%, crude fiber content is 10%;
(2) actication of culture: (specification is living bacteria count 2 × 10 by Active Dry Yeast
10individual/g) 2.4kg, add in 50kg syrup the active dry yeast stirring evenly activation and must activate for 25 minutes, syrup temperature is 33 DEG C, and in syrup, the mass percentage content of sucrose is 3%;
(3) inoculation mixing: gained material in the active dry yeast activated, proteolytic enzyme (specification is 50000u/g) 3kg, cellulase (specification is 50000u/g) 1.35kg, molasses 31.5kg and step (1) is mixed to obtain seed culture fluid;
(4) temperature controlled fermentation: control temperature is 30 DEG C, ferments 24 hours, period stirring once, keep air unobstructed, wherein containing yeast viable count 10 × 10
8cfu/g siccative;
(5) enzymolysis broken wall: continued to pile up by fermentation culture and heat up, control temperature is 40 DEG C, enzymolysis 50 hours, its medium and small peptide content 10% weight, yeast sporoderm-broken rate 89.4%, and crude fiber content is 6.7%;
(6) granulation: adopt screw extruding mode that fermentation materials is carried out granulation and obtain yeast culture composition granule, yeast culture grain diameter 6.4mm;
(7) dry: be 50 DEG C by yeast culture Grain size controlling temperature, drying 25 hours must product, and now the moisture mass percent of product is 11.7%;
(8) product aftertreatment: pack after the examination and test of products, obtains finished product.
The present invention is by carrying out fermentation enzymolysis processing by brewer's grains, and final drying forms product, develops high-quality feed raw material, has great significance.
The present invention is without the need to removing brewer's grains rice husk, and obtained yeast culture adopts extruder grain and oven drying at low temperature method to obtain product, shortens the production cycle, and processing parameter is clear and definite, it is characterized in that after the first step fermentation, yeast viable count>=10 × 10
8cfu/g siccative, little peptide content>=10% weight of yeast culture, yeast sporoderm-broken rate>=75%, technique is complete, and obtained finished product is of high nutritive value, and improves the feeding value of product.
The present invention adopts two Buwen's degree to control, and reaches protein, the abundant enzymolysis of Mierocrystalline cellulose and yeast broken wall, the object of releasing nutrients material.The first step control temperature is 20 ~ 50 DEG C, ferments 12 ~ 36 hours, keeps ventilating, and stirring once; Pile up after second step stirring and heat up, control temperature 35 ~ 65 DEG C, enzymolysis 12 ~ 60 hours.
The present invention is compared with existing brewer's grains zymotechnique, and tool has the following advantages and beneficial effect:
1, the fermentation enzymolysis process adopting the bacterium enzyme combined with yeast and proteolytic enzyme, cellulase to work in coordination with, technology stability is good, without mould contamination, product safety.
2, adopt two step method temperature control process, after cultivating, yeast count is high, thorough enzymolysis, and yeast broken wall is abundant, and nutritive value is higher.
3, this product coarse fibre content is more poor and like product is low by more than 50% than conventional beer, solves the present situation that conventional beer grain product can only be suitable for ruminating animal, has widened product and used face, be conducive to the developing in market.
Therefore, the present invention relates to a kind of take brewer's grains as the yeast culture extruder grain fermentation process of raw material, belongs to technical field of bioengineering.This fermentation enzymolysis process is main raw material with brewer's grains, with yeast saccharomyces cerevisiae be fermented bacterium basis on, associated proteins enzyme and cellulase, adopt the explained hereafter food yeast culture that fermentation enzymolysis is collaborative.Its main technique is: brewer's grains regulate water content 35 ~ 60% through adding the raw material such as wheat bran and dregs of beans, crude protein content is 18 ~ 35%, crude fiber content is 8 ~ 12%, inoculate activated yeast saccharomyces cerevisiae, and add proteolytic enzyme, cellulase and carbon source, fermentation materials 20 ~ 50 DEG C is cultivated 12 ~ 36 hours, stirring once, continue to pile up and heat up, control temperature 35 ~ 65 DEG C of enzymolysis 12 ~ 60 hours, make yeast broken wall, the raw material such as protein, Mierocrystalline cellulose is fully degraded, then granulation, dries 12 ~ 30 hours at 45 ~ 75 DEG C, defines yeast culture composition granule.
Present invention process is simple, reliable product quality is stablized, increase substantially the nutritive value of brewer's grains, significantly reduce cellulosic content in brewer's grains, animal health growth needs can be met, nutritive equilibrium, is the safety vegetable type feedstuff raw material of super quality and competitive price, can substitutes the animal feedstuff raw materials such as bone meal, fish meal, plasma proteins.
In sum, of the present invention is that the yeast culture extruder grain fermentation process of raw material can make full use of brewer's grains with brewer's grains, thus reduces environmental pollution, and simple to operate, cost is low, can be mass-produced, and is suitable for large-scale promotion application.
One skilled in the art will understand that the embodiments of the invention shown in foregoing description only limit the present invention as an example and not.
As can be seen here, object of the present invention is complete and be effectively achieved.Function of the present invention and structural principle are shown in an embodiment and are illustrated, do not deviating under described principle, embodiment can do any amendment.So, present invention comprises all variant embodiment based on claim spirit and right.
Claims (10)
1. one kind take brewer's grains as the yeast culture extruder grain fermentation process of raw material, it is characterized in that, in described brewer's grains, inoculate yeast saccharomyces cerevisiae, and add proteolytic enzyme, cellulase and carbon source, through fermentation and enzymolysis, then granulation dry thus make yeast culture composition granule.
2. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, before described inoculation, the water content of described brewer's grains is adjusted to 35 ~ 60% weight, the crude protein content of described brewer's grains is adjusted to 18 ~ 35% weight.
3. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 2 with brewer's grains, it is characterized in that, described adjustment regulates material to realize by adding.
4. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, described yeast saccharomyces cerevisiae is activated by Active Dry Yeast and forms.
5. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 4 with brewer's grains, it is characterized in that, the inoculum size of described Active Dry Yeast is every gram of described brewer's grains siccative inoculation 2 ~ 5 × 10
7individual; The addition of described proteolytic enzyme is the protein interpolation 100 ~ 500u in every gram of described brewer's grains; The addition of described cellulase is the robust fibre interpolation 150 ~ 400u in every gram of described brewer's grains; The addition of described carbon source is the 1-3% weight of described brewer's grains.
6. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, the temperature of described fermentation is 20 DEG C ~ 50 DEG C, and the time of described fermentation is 12 ~ 36 hours.
7. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, the temperature of described enzymolysis is 35 ~ 65 DEG C, and the time of described enzymolysis is 12 ~ 60 hours.
8. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, described granulation adopts screw extruding mode to carry out.
9. be the yeast culture extruder grain fermentation process of raw material as claimed in claim 1 with brewer's grains, it is characterized in that, the temperature of described drying is 45 ~ 75 DEG C, and the time of described drying is 12 ~ 30 hours.
10. a yeast culture composition granule, is characterized in that, adopt is that the yeast culture extruder grain fermentation process of raw material is prepared from brewer's grains as described in any one of claim 1 ~ 9.
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| CN111513191A (en) * | 2020-04-24 | 2020-08-11 | 路德环境科技股份有限公司 | Fermentation method of yeast culture with Maotai-flavor distiller's grains as raw material |
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