CN102106466A - Feed yeast culture additives, preparation method and application thereof for high producing dairy cows - Google Patents
Feed yeast culture additives, preparation method and application thereof for high producing dairy cows Download PDFInfo
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- CN102106466A CN102106466A CN2009102174219A CN200910217421A CN102106466A CN 102106466 A CN102106466 A CN 102106466A CN 2009102174219 A CN2009102174219 A CN 2009102174219A CN 200910217421 A CN200910217421 A CN 200910217421A CN 102106466 A CN102106466 A CN 102106466A
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Abstract
The invention relates to feed yeast culture additives, the preparation method and application thereof for high producing dairy cows, which aims to overcome the defects that the antibiotic additives have poor influence on the quality and taste of animal products, and the safety problems are caused due to drug resistance and drug residue, which causes the application of the antibiotic additives in animal husbandry production to be limited greatly. The feed yeast culture additives comprise Saccharomyces cereviseae, and the preparation method comprises the following steps of rejuvenating the introduced saccharomyces cereviseae under the aseptic processing technical condition, conducting amplification culture of saccharomyces cereviseae under the aseptic processing condition, fermenting, drying, and then fabricating a finished product through a pelletizer. The feed yeast culture additives are used for feed additives.
Description
Technical field:
What the present invention relates to is a kind of feed addictive that improves ruminant animal nutrition, particularly a kind of preparation method of high-yield milk cow feed yeast cultivating additive and application.
Background technology:
Since nearly half a century, huge effect has been brought into play in the development that antibiotic is produced herding; But follow the raising of people to food security consciousness, the antibiotics additive occurs the harmful effect and the safety issues such as drug resistance and medicament residue of livestock products quality and local flavor etc. thereupon, and it is very limited in application in animal husbandry; It is imperative to seek nontoxic pair, noresidue and free of contamination natural green feed addictive.
Summary of the invention:
The purpose of this invention is to provide a kind of can directly adding in the feed, can stablize microorganism bowed pastern balance in the cud, the concentration of cellulose-decomposing bacteria group and anaerobic bacteria in the raising cud, improve volatile fatty acid VFA output and utilization rate, change volatile fatty acid VFA ratio, made the additive of ruminal pH value rising, the present invention also provides the preparation method of this additive, realization is improved food conversion ratio and the digestion and the absorption of diet nutrient material, and then improves the milk cow milk production and improve the purpose of milk-quality.
Above-mentioned purpose realizes by following technical scheme:
The preparation method of high-yield milk cow feed yeast cultivating additive; its composition comprises: saccharomyces cerevisiae; the saccharomyces cerevisiae of introducing is carried out the rejuvenation of saccharomyces cerevisiae under the aseptic technique condition; under the condition of sterile working, carry out saccharomyces cerevisiae and enlarge cultivation; insert fermentation, and carry out the drying back and make finished product by comminutor.
The preparation method of described high-yield milk cow feed yeast cultivating additive, described rejuvenation are that it is changed in the test tube that sterilized solid medium is housed, and cultivate 3-5 days under 30 ℃ of constant temperatures; 4 ℃ of preservations; Described saccharomyces cerevisiae enlarges to be cultivated: inject aseptic 0.9% physiological saline in the test tube of cultured saccharomyces cerevisiae, bacteria suspension is made in concussion, the capacity of being inoculated into is in 1 liter the conical flask that 250 milliliters of saccharomyces cerevisiae fluid nutrient mediums are housed then, cultivates 36 hours for 30 ℃ in the isothermal vibration incubator.
The preparation method of high-yield milk cow feed yeast cultivating additive, described solid medium: at first maize straw, brewex's grains and corn flour are mixed in 20: 75: 5 ratio, then its mixture is crushed to the 60-80 order, again with itself and water in 1: 1 abundant mixing of ratio, put into 121 ℃ of autoclave sterilizations 30 minutes, take out the back at last and under nature, cool off.
The preparation method of described high-yield milk cow feed yeast cultivating additive, described access fermentation: will enlarge nutrient solution with 35% access amount, and be inoculated into and stir in the solid medium of sterilization afterwards 27 ℃-30 ℃ condition bottom fermentation 48-72 hour.
A kind of preparation method of above-mentioned high-yield milk cow feed yeast cultivating additive, described drying is solid medium to be put into 40 ℃ of-50 ℃ of baking ovens dry, drying time is 25 minutes; Described by comminutor make finished product be with corn flour and water be mixed into paste; cultured solid medium is put into wherein; fully mix at 1: 1 by weight; once more its mixture is put into the oven dry in 20 minutes of 40 ℃ of-50 ℃ of baking ovens; at last mixture is poured in the granulation machine, under 45 ℃ of conditions, carried out low temperature and granulate.
A kind of high-yield milk cow feed yeast cultivating additive that utilizes above-mentioned method to make.
A kind of above-mentioned application of additive in high-yield milk cow feed.
Beneficial effect:
1. the present invention can improve function of rumen fully, the interior profitable strain quantity of its cud is rolled up, and protozoon quantity reduces, and promotes interior microorganism of cud and flora can breed propagation in a large number, keep the interior microbiota of cud and get balance, increase the quantity of cellulose-decomposing bacteria.
2. the present invention is because the increase of cellulose-decomposing bacteria, the roughage of daily ration can be decomposed fully, the digestibility that has improved concentrate supplement improves, daily ration is is fully digested and assimilated in vivo, and then improve food conversion ratio, the augment nutritional composition is is fully digested and assimilated, and finally reaches the purpose of the production performance that improves animal.
The average daily milk yield of control group is 21.05kg/d, and the average daily milk yield of test group is 25.25kg/d.Therefore the test group of adding yeast culture increases 4.20kg/d than the average milk production of control group.
3. yeast culture is a kind of feed addictive that comprises yeast cells and grown cultures agent thereof, it is a kind of direct microorganism fodder, directly microorganism fodder is meant that these microorganisms comprise bacterium, fungi, yeast etc. by can directly feeding to a kind of product of animal that microbial cell is formed.The brewer's yeast culture of utilization of the present invention stimulates some beneficial bacterium growth to improving the microorganism ecological environment of ruminant tumor gastric, promotes that the effect of the growth of animal and the raising output of milk is comparatively obvious.
Based on the national conditions of China, the antibiotics additive has been listed in restriction and has used product in animal feed, substitutes one of antibiotic staple product so yeast culture has become, and its actual production meaning is far-reaching.It has, and cost of material is low, and product is stable, and long shelf-life provides stable properties of product, also contains multiple nutritional components, as other nutriments such as rich in amino acid, vitamin, enzymes, has the required nutriment of cow rumen growth of microorganism.Also having nontoxic pair, noresidue and free of contamination characteristics when promoting livestock birds health and production performance, is the feed addictive of natural green.
Add brewer's yeast or brewer's yeast culture for improving digesting and assimilating of nutriment in the milk cow diet, promote the performance of production performance and improve the milk cow health level, the aspects such as immunologic function that strengthen milk cow all have positive effect.Studies show that simultaneously, add the activity that the brewer's yeast culture can stimulate rumen microorganism in the daily ration, improve the stability of lumen fermentation, thereby improve the production performance of animal.The yeast culture that adds low dosage in the daily ration can improve the output of milk that can improve milk cow and improve milk-quality.Pass through great deal of experimental, show that yeast culture is to improving the microbial ecological of ruminant tumor gastric, stimulate some bacteria beneficial growth, promote that the effect of the growth of animal and the raising output of milk is comparatively obvious, and the trophism of ruminant obviously is better than nonruminant.
Contrast test: it is two kinds of 0mg/kg and 100mg/kg that described additive adds interpolation concentration in the finished product, and control group also be that concentration is added in 0mg/kg and two kinds of contrasts of 100mg/kg, by the daily ingestion amount interpolation of cattle farm milk cow.
The selection of described milk cow: the cattle farm amount of livestock on hand is 1500 lactating cows, and average milk production is at the 20kg/ head; 1000 cow heads of body condition scoring 3.5-4 basically identical, no mammitis and other diseases etc.; Be divided into 2 processed group and control group and test group at random, 500 of each processed group.
Test period is 180d; What adopt is model C S that Tianjin prosperous stockbreeding machine Co., Ltd the produces unit of milking, and goes up machine 2 times every day for the examination ox, and unit writes down lactation amount automatically, and 2 sums are the lactation amount of milk cow 1d; Record milk production every day is measured milk-quality after off-test, comprise protein ratio, butterfat percnetage, milk sugar rate and dry digestibility index.
1. butterfat percnetage:
Use the M301529 crema tester, survey once weekly.
2. lactoprotein, lactose, dry:
Adopt FT120 type dairy component analyzer to carry out newborn conventional determining;
The collection of breast sample: carry out the collection of newborn sample with test 90d and 180d respectively, suction pipe is from top to bottom slowly inserted at the bucket end, make milk liquid flow in the suction pipe naturally, adopt the sample breast of the required mensuration 50ml band plug plastic tube of packing into, each 50ml that gathers, gather the back sample and put and-20 ℃ of refrigerated storages, wait to be ready to use in newborn conventional determining;
Data statistics: test data is put in order with working software Excel, and variance analysis adopts data statistics software SPSS13.0 neutral line model GLM program to analyze; The average daily milk yield of control group is 21.05kg/d, and the average daily milk yield of test group is 25.25kg/d.Therefore the test group of adding yeast culture increases 4.20kg/d than the average milk production of control group.
The specific embodiment:
Embodiment 1:
The preparation method of high-yield milk cow feed yeast cultivating additive; its composition comprises: common saccharomyces cerevisiae saccharomycescerevisiae; the saccharomyces cerevisiae of introducing is carried out the rejuvenation of saccharomyces cerevisiae under the aseptic technique condition; under the condition of sterile working, carry out saccharomyces cerevisiae and enlarge cultivation; insert fermentation, and carry out the drying back and make finished product by comminutor.
Embodiment 2:
The preparation method of embodiment 1 described high-yield milk cow feed yeast cultivating additive, the rejuvenation of described bacterial classification: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube strains of being preserved, under 30 ℃ of constant temperatures, cultivate 3-5d; 4 ℃ of preservations were transplanted once in 4-5 month.
Described bacterial classification enlarges to be cultivated: under the condition of sterile working, carry out saccharomyces cerevisiae expansion cultivation and will inject aseptic 0.9% physiological saline in the cultured test tube, bacteria suspension is made in concussion, the capacity of being inoculated into is in the conical flask that the 300ml fluid nutrient medium is housed of 1L then, in the isothermal vibration incubator 30 ℃, 180r/min cultivates 36h.
Embodiment 3:
In the preparation method of embodiment 1 or 2 described high-yield milk cow feed yeast cultivating additives, described solid medium preparation, at first maize straw, brewex's grains and corn flour are mixed in 20: 75: 5 ratio, then its mixture is crushed to the 60-80 order, again with itself and water in 1: 1 abundant mixing of ratio, put into 121 ℃ of autoclaves 30min that sterilizes, take out the back at last and under nature, cools off.
Embodiment 4:
The preparation method of embodiment 1 or 2 described high-yield milk cow feed yeast cultivating additives, describedly insert fermentation: the brewer's yeast bacteria culture fluid that enlarges with 35% access amount, is inoculated into and stirs the back in the solid medium of sterilization at 27 ℃-30 ℃ condition bottom fermentation 48-72h; Carry out drying afterwards, its solid medium is put into 40 ℃ of-50 ℃ of baking ovens dry, drying time is for well about 25min.
The preparation method of high-yield milk cow feed yeast cultivating additive; it is described that to make finished product by comminutor be that corn flour is mixed with water; stir into paste for good too dense not thick or too thin; cultured solid medium is put into wherein; fully mix at 1: 1 by weight, once more its mixture is put into the oven dry in 20 minutes of 40 ℃ of-50 ℃ of baking ovens, mixture is poured in the granulation machine at last; carry out low temperature and granulate under 45 ℃ of conditions, particle diameter is relatively good in the 8mm left and right sides.
Claims (7)
1. the preparation method of a high-yield milk cow feed yeast cultivating additive; its composition comprises: saccharomyces cerevisiae; it is characterized in that: the saccharomyces cerevisiae of introducing is carried out the rejuvenation of saccharomyces cerevisiae under the aseptic technique condition; under the condition of sterile working, carry out saccharomyces cerevisiae and enlarge cultivation; insert fermentation, and carry out the drying back and make finished product by comminutor.
2. the preparation method of high-yield milk cow feed yeast cultivating additive according to claim 1 is characterized in that: described rejuvenation is that it is changed in the test tube that sterilized solid medium is housed, and cultivates 3-5 days under 30 ℃ of constant temperatures; 4 ℃ of preservations; Described saccharomyces cerevisiae enlarges to be cultivated: inject aseptic 0.9% physiological saline in the test tube of cultured saccharomyces cerevisiae, bacteria suspension is made in concussion, the capacity of being inoculated into is in 1 liter the conical flask that 250 milliliters of saccharomyces cerevisiae fluid nutrient mediums are housed then, cultivates 36 hours for 30 ℃ in the isothermal vibration incubator.
3. according to the preparation method of claim 2 high-yield milk cow feed yeast cultivating additive, it is characterized in that: described solid medium: at first maize straw, brewex's grains and corn flour are mixed in 20: 75: 5 ratio, then its mixture is crushed to the 60-80 order, again with itself and water in 1: 1 abundant mixing of ratio, put into 121 ℃ of autoclave sterilizations 30 minutes, take out the back at last and under nature, cool off.
4. the preparation method of high-yield milk cow feed yeast cultivating additive according to claim 3, it is characterized in that: described access fermentation: will enlarge nutrient solution with 35% access amount, and be inoculated into and stir in the solid medium of sterilization afterwards 27 ℃-30 ℃ condition bottom fermentation 48-72 hour.
5. a claim 1 or 2 or the preparation method of 3 or 4 described high-yield milk cow feed yeast cultivating additives, it is characterized in that: described drying is solid medium to be put into 40 ℃ of-50 ℃ of baking ovens dry, and drying time is 25 minutes; Described by comminutor make finished product be with corn flour and water be mixed into paste; cultured solid medium is put into wherein; fully mix at 1: 1 by weight; once more its mixture is put into the oven dry in 20 minutes of 40 ℃ of-50 ℃ of baking ovens; at last mixture is poured in the granulation machine, under 45 ℃ of conditions, carried out low temperature and granulate.
6. high-yield milk cow feed yeast cultivating additive that utilizes claim 1,2,3,4,5 method to make.
7. the application of additive in high-yield milk cow feed that utilizes claim 6.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103549116A (en) * | 2013-10-29 | 2014-02-05 | 江苏谷硅新材料股份有限公司 | Process for preparing yeast culture by taking hulled spirit distilled grains as raw material |
| CN105087404A (en) * | 2015-09-11 | 2015-11-25 | 宿迁市爱维贝生物科技有限公司 | Extruding granulation fermentation method of yeast culture prepared from brewer's grains and related yeast culture particles |
| CN105166327A (en) * | 2015-10-20 | 2015-12-23 | 江苏益元泰生物技术有限公司 | Movable fermentation process for preparing wet-state yeast culture taking brewer's grains as raw materials |
-
2009
- 2009-12-24 CN CN2009102174219A patent/CN102106466A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103549116A (en) * | 2013-10-29 | 2014-02-05 | 江苏谷硅新材料股份有限公司 | Process for preparing yeast culture by taking hulled spirit distilled grains as raw material |
| CN103549116B (en) * | 2013-10-29 | 2015-04-22 | 江苏谷硅新材料股份有限公司 | Process for preparing yeast culture by taking hulled spirit distilled grains as raw material |
| CN105087404A (en) * | 2015-09-11 | 2015-11-25 | 宿迁市爱维贝生物科技有限公司 | Extruding granulation fermentation method of yeast culture prepared from brewer's grains and related yeast culture particles |
| CN105166327A (en) * | 2015-10-20 | 2015-12-23 | 江苏益元泰生物技术有限公司 | Movable fermentation process for preparing wet-state yeast culture taking brewer's grains as raw materials |
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Application publication date: 20110629 |