CN105037516A - 玉米oxs2基因家族、其编码蛋白及应用 - Google Patents
玉米oxs2基因家族、其编码蛋白及应用 Download PDFInfo
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Abstract
本发明公开了玉米OXS2基因家族、其编码蛋白及应用。玉米OXS2蛋白家族中的ZmOXS2b的氨基酸序列如SEQ？ID？NO.2所示，其编码基因如SEQ？ID？NO.1所示；ZmO2L1的氨基酸序列如SEQ？ID？NO.4所示，其编码基因如SEQ？ID？NO.3所示。本发明所克隆到的两个玉米OXS2基因都可以被重金属胁迫诱导。酵母抗性检测表明，这两个基因的异源表达可增强酵母抗氧化剂二酰胺的能力，ZmOXS2b的功能片段AT3也可以增强酵母抗二酰胺的能力。当它们在拟南芥中大量表达时，可以增强拟南芥抗镉（Cd）的能力。该家族的另外一个成员ZmOXS2a与其他两个成员高度同源，预测具有相同的功能。因此，这三个基因将具有极大的潜力应用于培育具有抗逆特性的转基因作物中，从而提高粮食作物的产量。
Description
技术领域
本发明属于分子生物学领域，具体涉及玉米OXS2基因家族、其编码蛋白及应用。
背景技术
目前，世界上的许多耕地都有轻度的重金属污染，这些重金属污染物主要有镉、铜、锌、镍、钴、铅、砷等。这些重金属污染主要是由于长期使用磷酸肥料、污水处理不利、工业废料的污染、农业灌溉的不科学造成的。植物体在面对这些重金属胁迫时一方面会产生活性氧（ROS）。在植物中，大多数金属产生活性氧（ROS）是重金属毒性的间接作用结果。这种间接的作用包括它们与抗氧化系统的反应，扰乱电子传递链，或者扰乱新陈代谢的必需元素的合成。植物体中，重金属胁迫所造成的另一个较严重的结果就是脂质的过氧化，这种脂质的过氧化可以导致生物膜的损伤。丙二醛(MDA)是生物膜中多不饱和脂肪酸的一种重要的分解产物，可以用来作为生物膜氧化胁迫的指示剂。重金属会导致植物体出现很多的病症。以镉为例，镉(Cd)是一种高毒性的重金属。镉处理会抑制植物的很多生理过程，比如光合作用、细胞延长、固氮和矿物营养吸收等。耕地中镉的正常含量不能超过100mg/kg。植物体处于镉胁迫时，就会出现光合效率降低、水分吸收降低以及营养吸收降低等性状。植物在含有过多的隔的土壤中，会出现萎黄病、生长抑制并最终导致植物体死亡。
玉米（学名：Zeamays）是重要的粮食作物和饲料来源，也是全世界总产量最高的粮食作物，目前也是我国种植面积最大的农作物。现代科技条件下，玉米深加工被广泛应用于食品工业，医药工业和化学工业，具有广阔的应用前景。但是，随着耕地污染的加重，各种主要的农作物的产量，包括玉米在内都受到严重的影响。所以，研究玉米中的抗性基因及其功能，对于提高玉米以及其他粮食作物的增产具有重大意义。
发明内容
本发明的目的在于提供玉米OXS2基因家族、其编码蛋白及应用。
本发明所采取的技术方案是：
玉米OXS2蛋白，其含有如下（1）-（4）任一项所示的氨基酸序列：
（1）SEQIDNO.2所示的氨基酸序列；
（2）SEQIDNO.4所示的氨基酸序列；
（3）SEQIDNO.6所示的氨基酸序列；
（4）SEQIDNO.2或SEQIDNO.4或SEQIDNO.6所示的氨基酸序列经取代、缺失和/或增加一个或多个氨基酸和/或末端修饰后且具有提高植物抗逆功能的序列。
编码玉米OXS2蛋白的基因。
玉米OXS2蛋白AT3片段，其含有如下（1）-（3）任一项所示的氨基酸序列：
（1）SEQIDNO.7所示的氨基酸序列；
（2）SEQIDNO.9所示的氨基酸序列；
（3）SEQIDNO.7或SEQIDNO.9所示的氨基酸序列经取代、缺失和/或增加一个或多个氨基酸和/或末端修饰后且具有提高植物抗逆功能的序列。
编码AT3片段的基因。
含有玉米OXS2蛋白编码基因序列或AT3片段编码基因序列的重组载体、重组菌或转基因细胞系、转基因植物株系。
玉米OXS2蛋白、其编码基因或AT3片段、其编码基因在培育抗逆植物中的应用。
玉米OXS2蛋白、其编码基因或AT3片段、其编码基因在抗逆植物辅助育种中的应用。
所述抗逆性状包括抗重金属污染，抗氧化胁迫、抗高温胁迫、抗低温胁迫、抗盐碱、抗旱、抗病虫害中的至少一种。
所述重金属包括镉、汞、金、银、铜、铁、铅、砷、铬中的至少一种。
一种抗逆植物的培育方法，包括将玉米OXS2蛋白编码基因序列或AT3片段编码基因序列转入受体植物中，得到转基因植物；所述转基因植物与受体植物相比，其对逆境的抗性提高。
本发明的有益效果是：
本发明在玉米中克隆了OXS2的两个同源基因ZmOXS2b与ZmO2L1。这两个基因的表达都可以被重金属胁迫诱导。当它们在拟南芥中大量表达时，可以特异性地增强拟南芥抗重金属镉（Cd）的能力。因此，这两个基因将具有极大的潜力应用于培育具有抗重金属的转基因作物中，从而提高粮食作物的产量。
附图说明
图1为ZmOXS2b与ZmO2L1基因PCR产物的1%的琼脂糖凝胶电泳图；
图2为玉米中ZmOXS2b与ZmO2L1的基因结构示意图（蓝色长方型：ANKYRIN重复序列；红色长方形：锌指结构域；绿色方框：多聚谷氨酰胺序列；倒三角：预测出核序列；黑色长条：AT3片段；所有蛋白编码区中并不存在内含子。数字表示从转录起始位点开始的DNA基因组位置）；
图3为qRT-PCR检测的ZmOXS2b与ZmO2L1的相对表达量（生长15天的玉米植株使其处于0或者200μMCdCl2处理下，误差线表示三次独立实验的标准差）；
图4为滴板检测ZmOXS2b，ZmO2L1、AT3片段在粟酒裂殖酵母中的组成型表达（用转化空载体的酵母作为负对照，三角形表示，浓度依次十倍稀释）；
图5拟南芥种子种植在水平放置的1/2MS与含有75μMCdCl2浓度的1/2MS培养基上11天（每种转基因植株的三个独立的转基因株系被用来检测）；
图6为生长在1/2MS与含有75μMCdCl2的1/2MS培养基上11天的拟南芥幼苗（5棵）的鲜重（每个株系不少于20棵幼苗被用来测量，误差线表示三次独立重复实验的标准偏差）；
图7为拟南芥种子种植在竖直放置的1/2MS与含有75μMCdCl2浓度的1/2MS培养基上11天（每种转基因植株的三个独立的转基因株系被用来检测）。
具体实施方式
下面结合实施例对本发明作进一步的说明，但并不局限于此。
以下实施例中所采用的分子生物学实验技术包括PCR扩增、质粒提取、质粒转化、DNA片段连接、酶切、凝胶电泳等，如无特殊说明，通常按照常规方法操作，具体可参见《分子克隆实验指南》(第三版)(SambrookJ,RussellDW，JanssenK,ArgentineJ.黄培堂等译，2002，北京：科学出版社)，或按照制造厂商所建议的条件。
一、材料与方法
1、生物材料与处理：
本实验采用的玉米品种为中国岭南广泛种植的甜玉米。野生型拟南芥（Arabidopsisthaliana）采用Columbia（Col-0）生态型。
本实验基因的克隆与玉米的Cd处理均采用华南地区广泛种植的品种：丰甜1号。在玉米种子发芽9天（5天浸泡于水中，4天置于空气中）后，将种子转移至MS水状态培养基上生长。生长条件：22℃，16/8光周期。生长过程中，1天补充2次氧气，每次半小时。当玉米在水培养基上生长五天后，将所有的小植物分为两部分，一部分放置于含有200μMCdCl2的MS水培营养液中，一部分更换不含Cd的营养液正常生长，同时，开始以置换营养液的时间为零点，以此后的0小时、3小时、6小时、12小时、24小时、48小时开始收集植株的叶片。每3个植株的叶片为一个样品，液氮速冻，-80℃储存。
2、转基因拟南芥的获得
拟南芥种子用1比13的次氯酸钠消毒两次共8分钟，再用无菌水洗6次共六分钟，种于MS培养基。含有种子的MS培养基放入4℃冰箱春化3d，然后置于光照培养箱中以20℃16h、20℃8h为一光周期培养。培养10d后将幼苗移至营养土：蛭石：珍珠岩=1：1：1的塑料盒中定期浇水培养至开花，然后用于基因转化。
运用电转法将带有ZmOXS2与ZmO2L基因的表达载体pCambia3300导入根癌农杆菌GV3101中。筛选抗利福平和卡那霉素的农杆菌进行菌落PCR。挑选阳性的菌落摇菌，扩配，用浸花法浸染拟南芥。在含100μg/mL除草剂（ppt）的1/2MS培养基上筛选T0的种子（T1代幼苗），取T1代和Col野生型植株的叶片提取DNA，以野生型与突变体为阴性对照进行PCR鉴定，选取阳性株。每种转基因拟南芥得到大约十株T1代植株，通过分离比，筛选这四种转基因拟南芥的单位点插入纯合体种子保存，并从中对每个转化随机挑选3个株系作为后续研究的材料再进行抗性检测。我们对作为研究材料所有独立株系进行RT-PCR验证，发现转基因均有较高的表达。
3、酵母的抗性检测
在长有酵母的EMM固体筛选培养基上挑选菌落，加入到含有3-5mlEMM液体筛选培养基的50ml离心管中，充分分散、混匀。250rpm，30℃，摇床上摇2-18小时（OD>0.3）。稀释母液至OD=0.1，250rpm，30℃，摇床上摇4-8小时，使OD值略大于0.3，稀释调节OD值至0.3。以0.3OD的酵母液为母液，分别稀释到1/10，1/100，1/1000倍不同浓度梯度，各取3μL溶液，依次滴至含有不同浓度diamide，H2O2，Cd的EMM固体筛选培养基上。30℃倒置培养5-7天。
二、实验结果
1、玉米OXS2基因的克隆
以中国南方地区广泛种植的甜玉米丰甜1号为材料，提取其基因组，并以其基因组DNA为模板，设计引物克隆广东甜玉米中的 ZmOXS2b与ZmO2L1基因，引物序列如表1所述。利用高保真DNA聚合酶（High-FidelityDNAPolymerase）PCR反应体系。取50μLPCR产物进行1%的琼脂糖凝胶电泳分析，如图1所述。取目标条带进行测序，所得ZmOXS2b基因的核苷酸序列如SEQIDNO.1所示，其所编码的蛋白序列如SEQIDNO.2所示；ZmO2L1基因的核苷酸序列如SEQIDNO.3所示，其所编码的蛋白序列如SEQIDNO.4所示。
表1PCR引物
2、玉米OXS2基因家族信息
如图2所示，ZmOXS2b与ZmO2L1蛋白都含有两个锚蛋白重复序列和两个锌指结构域。
3、玉米OXS2家族基因响应重金属Cd诱导
为了检验ZmOXS2家族基因在玉米中的表达情况，我们用水培的方法将刚发芽9天的玉米植株种植在营养液中，一半的样品用含有200μMCdCl2的MS营养液处理，一半在无CdCl2的MS营养液中生长。在Cd处理后的0，3，6，12，24，48小时收集三棵植株的叶片。qRT-PCR的实验技术用来检测这两个基因的表达情况。如图3所示，在没有胁迫处理的条件下，ZmOXS2家族基因在不同的时间点表达的水平较一致，其中，ZmO2L1的表达水平较高。然而，在转移至含有CdCl2的MS培养基的3小时后，ZmOXS2b与ZmO2L1的表达量与未处理的样品相比升高了大约两倍。随着处理时间的延长，ZmOXS2b的表达量逐步下降，然而ZmO2L1的表达量在Cd处理的第24小时达到最高值，48小时又有回落。综合来看ZmOXS2b与ZmO2L1可以被Cd瞬时激活。这表明，ZmOXS2家族基因有潜在的功能可能参与植物中重金属胁迫抗性的调控。
4、玉米OXS2家族基因在酵母中的抗性检测
我们克隆了ZmOXS2b和ZmO2L1基因全长，并克隆到酵母表达载体pART1中，转化酵母。如图4所示，在酵母中过量表达ZmOXS2b与ZmO2L1可以增强酵母对二酰胺的抗性。因为化学物质二酰胺能够直接引起氧化反应，所以ZmOXS2b与ZmO2L1有可能在氧化胁迫响应机制上起作用。
我们进一步在ZmOXS2b与ZmO2L1中筛选出一段AT3序列，并将这些小片段的DNA序列加上启动子ATG转入酵母表达载体pART1中，在酵母中验证其抗性。如图4所示，抗性检测结果表明，ZmOXS2b的AT3片段的转基因酵母具有抗二酰胺氧化胁迫的能力。因此，我们推测玉米OXS2家族基因的AT3片段与玉米的抗性相关，具有极大的潜力应用于培育具有抗逆性状的转基因作物中。
ZmOXS2b的AT3片段如SEQIDNO.7所示，其编码的核苷酸序列如SEQIDNO.8所示；ZmO2L1的AT3片段如SEQIDNO.9所示，其编码的核苷酸序列如SEQIDNO.10所示。
5、玉米OXS2家族基因在拟南芥中的抗性检测
为了进一步在植物中验证ZmOXS2家族基因在植物中的功能，我们将其转化拟南芥以进行抗性检测。通过花序侵染法，得到大约各10株以Col为背景的ZmOXS2b与ZmO2L1过表达转基因拟南芥的独立株系。通过分离比，筛选这四种转基因拟南芥的单位点插入纯合体种子保存，并从中对每个转化随机挑选3个株系作为后续研究的材料。我们对作为研究材料所有独立株系进行RT-PCR验证，发现转基因均有较高的表达。
随后，我们对这些转基因拟南芥WT(ZmOXS2b）和WT(ZmO2L1)以及它们的野生型对照进行了胁迫的处理。如图5和图6所示，在正常的生长条件下，野生型转基因株系生长状况一致。当用的75μMCdCl2处理时，以野生型为背景过量表达ZmOXS2b与ZmO2L1的转基因拟南芥的叶子的伸长状态明显好于野生型，同时转基因植株的鲜重也明显高于野生型(图6)。当培养基在同种条件下垂直放置时，以Col为背景过量表达ZmOXS2b与ZmO2L1的转基因拟南芥的根的生长状态也明显的好于野生型(图7)。
以上实验结果表明，在拟南芥中大量表达ZmOXS2b与ZmO2L1基因，可以特异性地增强拟南芥抗重金属镉（Cd）的能力。因此，这两个基因将具有极大的潜力应用于培育具有抗逆性状的转基因作物中，从而提高粮食作物的产量。
我们再后续的实验中发现了玉米OXS2家族基因中的另一个同源基因ZmOXS2a，其核苷酸序列如SEQIDNO.5所示，其编码的氨基酸序列如SEQIDNO.6所示。经序列分析，ZmOXS2a与ZmOXS2b、ZmO2L1具有较高的同源性。因此我们预测ZmOXS2a基因同样具有极大的潜力应用于培育具有抗逆性状的转基因作物中，从而提高粮食作物的产量。
<110>中国科学院华南植物园
<120>玉米OXS2基因家族、其编码蛋白及应用
<130>
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<213>sweetcorn（ZmOXS2b）
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<213>sweetcorn（ZmO2L1）
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gacatgaccgctgctgctgctgctctagggcttctacctggatctcctaccaggcacttt1140
gtgccaccacctctgtcaccatctgctgccaacaatggtggaggtgctgctgctcattgg1200
ctccaaggtagcaggctgcgctcttctttcaatgcaagagatgcacaggctgatgacctt1260
ggctcgctcctggaatgggaatctcaataccttggagcgctcagcctcccacaaagcagc1320
cgatcccagccccgtctttctactggtctgactattcgtccaacagcagttgctccatcc1380
tacctcgaagagatgtatgcttcagacatggctatgtcgccaaggttcaccaatgaccaa1440
ggtcactcagtcttctcaccagcacacaaatcagctcttctaaacaagtttcatcatcaa1500
cagaagggccttttgtcgcctgtcaataccaacaggatgtactcaccaaggggccttgat1560
ccatcaattatccattctccatttggtggcatgtctcctcggtcccctcggactatggag1620
ctaacatctcccctaagtgtccgtgttggtgttggagctgctgttactccgcgtgatatg1680
ttggatcagttttcttccctgaacaagcaccaggtgccatctgtgggatcgccacggaat1740
cttaatgcctcatggggcaacattggtaccccaaagagtaaggtcgactggggtgtggac1800
gacgacgaactagtgcgcttgaggcaccctgtgcaacatggaaatacagaagaggaacca1860
gatgtgtcttgggtgcagtcactggtaaaccatgctgagctgaatggcaagaggggtgaa1920
atggcaagcatggcttctccatcgattaacaagcctgacctgagcttttag1971
<210>4
<211>656
<212>PRT
<213>sweetcorn（ZmO2L1）
<400>4
MetGlyAspLeuAlaAspLeuAlaGlyAlaGlyGluAlaAlaArgSer
151015
HisLeuAlaAlaGlyGlyGlnArgAspArgLeuAlaAlaLeuLeuGlu
202530
LeuAlaAlaAlaAspAspValAlaGlyPheArgGluAlaLeuAlaGly
354045
GlyGlyGlyGluGlyAspThrAlaGluLeuAlaAspGlyValGlyMet
505560
TrpTyrGlyArgSerLysAlaTyrGluProArgThrProLeuMetVal
65707580
AlaAlaThrTyrGlySerValGluValValSerLeuLeuLeuAlaLeu
859095
GlyCysValAspValAsnArgArgProGlyValAspGlyAlaThrPro
100105110
LeuHisCysAlaAlaSerGlyGlySerArgAsnAlaValAlaValVal
115120125
LysLeuLeuLeuGlyAlaGlyAlaAspProValThrProAspSerAla
130135140
GlyArgLeuProAlaAspValIleLeuSerProProAlaSerProAsp
145150155160
AlaLeuGlyAspLeuGluMetLeuLeuGlyArgArgArgGlyLeuAla
165170175
ValAlaThrSerValProSerLeuSerSerSerProProLeuSerSer
180185190
SerProAspGluSerAsnArgSerProSerSerArgSerSerSerLeu
195200205
SerProIleThrValAspArgAlaLysLysGluTyrProValAspPro
210215220
ThrLeuProAspIleLysSerArgValTyrAlaSerAspGluPheArg
225230235240
MetPheAlaPheLysValArgProCysSerArgAlaTyrSerHisAsp
245250255
TrpThrGluCysProPheValHisProGlyGluAsnAlaArgArgArg
260265270
AspProArgLysHisProTyrThrAlaValProCysProAsnPheArg
275280285
ArgProGlyGlyCysProSerGlyAspSerCysGluPheSerHisGly
290295300
ValPheGluSerTrpLeuHisProSerGlnTyrArgThrArgLeuCys
305310315320
LysGluGlyAlaAlaCysAlaArgArgIleCysPhePheAlaHisAsp
325330335
GluAspGluLeuArgHisValProHisAsnSerGlyAlaGlyLeuLeu
340345350
SerProArgAlaSerSerSerIleAspMetThrAlaAlaAlaAlaAla
355360365
LeuGlyLeuLeuProGlySerProThrArgHisPheValProProPro
370375380
LeuSerProSerAlaAlaAsnAsnGlyGlyGlyAlaAlaAlaHisTrp
385390395400
LeuGlnGlySerArgLeuArgSerSerPheAsnAlaArgAspAlaGln
405410415
AlaAspAspLeuGlySerLeuLeuGluTrpGluSerGlnTyrLeuGly
420425430
AlaLeuSerLeuProGlnSerSerArgSerGlnProArgLeuSerThr
435440445
GlyLeuThrIleArgProThrAlaValAlaProSerTyrLeuGluGlu
450455460
MetTyrAlaSerAspMetAlaMetSerProArgPheThrAsnAspGln
465470475480
GlyHisSerValPheSerProAlaHisLysSerAlaLeuLeuAsnLys
485490495
PheHisHisGlnGlnLysGlyLeuLeuSerProValAsnThrAsnArg
500505510
MetTyrSerProArgGlyLeuAspProSerIleIleHisSerProPhe
515520525
GlyGlyMetSerProArgSerProArgThrMetGluLeuThrSerPro
530535540
LeuSerValArgValGlyValGlyAlaAlaValThrProArgAspMet
545550555560
LeuAspGlnPheSerSerLeuAsnLysHisGlnValProSerValGly
565570575
SerProArgAsnLeuAsnAlaSerTrpGlyAsnIleGlyThrProLys
580585590
SerLysValAspTrpGlyValAspAspAspGluLeuValArgLeuArg
595600605
HisProValGlnHisGlyAsnThrGluGluGluProAspValSerTrp
610615620
ValGlnSerLeuValAsnHisAlaGluLeuAsnGlyLysArgGlyGlu
625630635640
MetAlaSerMetAlaSerProSerIleAsnLysProAspLeuSerPhe
645650655
<210>5
<211>2241
<212>DNA
<213>corn（ZmOXS2a）
<400>5
atgaacggcatgccgatctctgcgtcctctgcggcaagcgtcgacagtggcgcggtggcg60
gccggcacgcccatgaagaatgcaacggcggcggacgctctcgccgagatggcgagacat120
cttaccgtcgacacggaagacaccttcgcgggcctgctggagctcgccgctgacgacgac180
gcagaggggctgcgcctcgcgctggagcgcgcgccacctgccgccgcggacgaggctggc240
ctctggtacggccgccggaaggtcctggagcaccgcaccccgctgatggtcgctgccacc300
tatgggagccttgccgcgctccgcctgctagtgtccatctcgtatgtggacgtcaaccgc360
cgctcgggcacggacggcaccactgccctccactgcgccgcctctggtggctcacggacg420
gctgttgagtcggtcaagctgcttctgggagccggggcagacgctaacaccatggatgac480
gccgggcgtcgtccggctgatgtgatctctgtgccaccgaagatgtttgatgccaagttc540
gcccttcaagatcttcttggattccccaagtctgagcatggcatgctccgggtggtgaca600
aggtccaccaactcgatctcatcgcctgtttcatcccccactgcagaggatgcacggtca660
ccgtctgcttctgtgatgatgatgacaaagttcgctgacttgcctagggttgcgacatcg720
gagaagaaggaatatccagtggatccatcccttccagacatcaagaacagcatatatgct780
tccgatgagttccgcatgtactcgttcaagatccgcccttgctcacgggcatactcgcat840
gactggactgagtgcccatttgtccacccaggagagaatgctcggcgccgagacccccgc900
aagtaccactacagttgtgtgccatgcccagatttcaggaagggcgtatgccggcgcgga960
gacatgtgtgaatatgctcatggagtgtttgagtgctggctccatccagcacagtaccgg1020
acgcgcctatgcaaggacggcacaagttgcaatcgtcgcgtctgtttctttgcccacaca1080
actgatgaactccgtccattgtatgtctcgactggatcagcggtgccatctccaagagct1140
tcagcaacagctgctatggagatggctgcagcaatgggcttgatgcccggttccccatca1200
tcggtttccgcggtaatgtccccgtttaccccaccaatgtccccttcgggcaatgggatg1260
cccccttcattgggctggcagcagccaaatgttccaactttacatcttcctggcagcagc1320
cttcagtcaagcaggctccgaacatcacttagtgcaagggatatgcctgctgatgactac1380
tccatgatgcgggatcttgattcccaacttatgaatgatctttgctattcccgtctcagt1440
tcatccacagggaaccactctgctagaaccaagtccctgaatccatcaaacctggatgac1500
ctcttctctgctgaaatgttatcatccccaaggtatagtaatgctgaccagggtgcaatg1560
ttttcaccttctcacaaggctgctatcctgaatcagttccagcagcagcagcaagcactg1620
ctgtctccaatcaacacaggagtttactctccaaagggcgtggacaaccagcagttacct1680
tcacactcatcgctgctgcaagcatcactcgggatgccctcccctggccggatgtctcca1740
cgatgtgtcgaatcgggctccccaatgagctcccacctagccgctgctcttgtccagcgg1800
gagaagcagcagcagacaatgagaagtctcagctctcgtgaccttggtcctagcgcggca1860
cgagcttcggctcttggctcccctctaagctcatcatggtctaagtggggatctccttcg1920
gggacaccagattggggcataaatggtgaagaattgggtaagctccggcgctcatcgtcc1980
ttcgagctgaggtctggtggtgacgaccctgatctctcttgggtgcatacactggtcaag2040
gaatctccaccggagaagcaagttactacggctgaatcaattagttccgttggaccctca2100
ccactaatgcctcccggcatgaacaatggcgacggttctggcctgagcacccagctggat2160
ggacacgaccaagccgcggttataggagcgttgcttgaacagatgcagcttgaccagcag2220
attggtagtctagcaacatag2241
<210>6
<211>746
<212>PRT
<213>corn（ZmOXS2a）
<400>6
MetAsnGlyMetProIleSerAlaSerSerAlaAlaSerValAspSer
151015
GlyAlaValAlaAlaGlyThrProMetLysAsnAlaThrAlaAlaAsp
202530
AlaLeuAlaGluMetAlaArgHisLeuThrValAspThrGluAspThr
354045
PheAlaGlyLeuLeuGluLeuAlaAlaAspAspAspAlaGluGlyLeu
505560
ArgLeuAlaLeuGluArgAlaProProAlaAlaAlaAspGluAlaGly
65707580
LeuTrpTyrGlyArgArgLysValLeuGluHisArgThrProLeuMet
859095
ValAlaAlaThrTyrGlySerLeuAlaAlaLeuArgLeuLeuValSer
100105110
IleSerTyrValAspValAsnArgArgSerGlyThrAspGlyThrThr
115120125
AlaLeuHisCysAlaAlaSerGlyGlySerArgThrAlaValGluSer
130135140
ValLysLeuLeuLeuGlyAlaGlyAlaAspAlaAsnThrMetAspAsp
145150155160
AlaGlyArgArgProAlaAspValIleSerValProProLysMetPhe
165170175
AspAlaLysPheAlaLeuGlnAspLeuLeuGlyPheProLysSerGlu
180185190
HisGlyMetLeuArgValValThrArgSerThrAsnSerIleSerSer
195200205
ProValSerSerProThrAlaGluAspAlaArgSerProSerAlaSer
210215220
ValMetMetMetThrLysPheAlaAspLeuProArgValAlaThrSer
225230235240
GluLysLysGluTyrProValAspProSerLeuProAspIleLysAsn
245250255
SerIleTyrAlaSerAspGluPheArgMetTyrSerPheLysIleArg
260265270
ProCysSerArgAlaTyrSerHisAspTrpThrGluCysProPheVal
275280285
HisProGlyGluAsnAlaArgArgArgAspProArgLysTyrHisTyr
290295300
SerCysValProCysProAspPheArgLysGlyValCysArgArgGly
305310315320
AspMetCysGluTyrAlaHisGlyValPheGluCysTrpLeuHisPro
325330335
AlaGlnTyrArgThrArgLeuCysLysAspGlyThrSerCysAsnArg
340345350
ArgValCysPhePheAlaHisThrThrAspGluLeuArgProLeuTyr
355360365
ValSerThrGlySerAlaValProSerProArgAlaSerAlaThrAla
370375380
AlaMetGluMetAlaAlaAlaMetGlyLeuMetProGlySerProSer
385390395400
SerValSerAlaValMetSerProPheThrProProMetSerProSer
405410415
GlyAsnGlyMetProProSerLeuGlyTrpGlnGlnProAsnValPro
420425430
ThrLeuHisLeuProGlySerSerLeuGlnSerSerArgLeuArgThr
435440445
SerLeuSerAlaArgAspMetProAlaAspAspTyrSerMetMetArg
450455460
AspLeuAspSerGlnLeuMetAsnAspLeuCysTyrSerArgLeuSer
465470475480
SerSerThrGlyAsnHisSerAlaArgThrLysSerLeuAsnProSer
485490495
AsnLeuAspAspLeuPheSerAlaGluMetLeuSerSerProArgTyr
500505510
SerAsnAlaAspGlnGlyAlaMetPheSerProSerHisLysAlaAla
515520525
IleLeuAsnGlnPheGlnGlnGlnGlnGlnAlaLeuLeuSerProIle
530535540
AsnThrGlyValTyrSerProLysGlyValAspAsnGlnGlnLeuPro
545550555560
SerHisSerSerLeuLeuGlnAlaSerLeuGlyMetProSerProGly
565570575
ArgMetSerProArgCysValGluSerGlySerProMetSerSerHis
580585590
LeuAlaAlaAlaLeuValGlnArgGluLysGlnGlnGlnThrMetArg
595600605
SerLeuSerSerArgAspLeuGlyProSerAlaAlaArgAlaSerAla
610615620
LeuGlySerProLeuSerSerSerTrpSerLysTrpGlySerProSer
625630635640
GlyThrProAspTrpGlyIleAsnGlyGluGluLeuGlyLysLeuArg
645650655
ArgSerSerSerPheGluLeuArgSerGlyGlyAspAspProAspLeu
660665670
SerTrpValHisThrLeuValLysGluSerProProGluLysGlnVal
675680685
ThrThrAlaGluSerIleSerSerValGlyProSerProLeuMetPro
690695700
ProGlyMetAsnAsnGlyAspGlySerGlyLeuSerThrGlnLeuAsp
705710715720
GlyHisAspGlnAlaAlaValIleGlyAlaLeuLeuGluGlnMetGln
725730735
LeuAspGlnGlnIleGlySerLeuAlaThr
740745
<210>7
<211>486
<212>DNA
<213>sweetcorn（ZmOXS2bAT3）
<400>7
ctgtctcctcgggccacggctatccctgagccagcgtctccgatgagctccaggctgctc60
gcggcccttgcgcagcgagagaagatgcagcagcagacgctgagaagcatgagctcccgt120
gaccttgcctctggcgcctccgtcctggttgggtcaccggttacctctagctggtccaaa180
tggggaatccccccgagcacaccagattggggtgctgacgacgaggaactcggccgcctc240
aagcgatcctcgtcctttgagctccggagcggtgccaacggagatgagccggacctctca300
tgggtcaatacccttgtgaaggagccgacgccggagaagccatccatcaacgggacaact360
gcaaaggagaccattgcttctttgagtgaggccgcgagtcatgaggacatcggcggcgag420
gacgacacagccggggtcatcggtggctggcttgaacagctccagctggatgagatggta480
gtctag486
<210>8
<211>161
<212>PRT
<213>sweetcorn（ZmOXS2bAT3）
<400>8
LeuSerProArgAlaThrAlaIleProGluProAlaSerProMetSer
151015
SerArgLeuLeuAlaAlaLeuAlaGlnArgGluLysMetGlnGlnGln
202530
ThrLeuArgSerMetSerSerArgAspLeuAlaSerGlyAlaSerVal
354045
LeuValGlySerProValThrSerSerTrpSerLysTrpGlyIlePro
505560
ProSerThrProAspTrpGlyAlaAspAspGluGluLeuGlyArgLeu
65707580
LysArgSerSerSerPheGluLeuArgSerGlyAlaAsnGlyAspGlu
859095
ProAspLeuSerTrpValAsnThrLeuValLysGluProThrProGlu
100105110
LysProSerIleAsnGlyThrThrAlaLysGluThrIleAlaSerLeu
115120125
SerGluAlaAlaSerHisGluAspIleGlyGlyGluAspAspThrAla
130135140
GlyValIleGlyGlyTrpLeuGluGlnLeuGlnLeuAspGluMetVal
145150155160
Val
<210>9
<211>459
<212>DNA
<213>sweetcorn（ZmOXSL1AT3）
<400>9
ttgtcgcctgtcaataccaacaggatgtactcaccaaggggccttgatccatcaattatc60
cattctccatttggtggcatgtctcctcggtcccctcggactatggagctaacatctccc120
ctaagtgtccgtgttggtgttggagctgctgttactccgcgtgatatgttggatcagttt180
tcttccctgaacaagcaccaggtgccatctgtgggatcgccacggaatcttaatgcctca240
tggggcaacattggtaccccaaagagtaaggtcgactggggtgtggacgacgacgaacta300
gtgcgcttgaggcaccctgtgcaacatggaaatacagaagaggaaccagatgtgtcttgg360
gtgcagtcactggtaaaccatgctgagctgaatggcaagaggggtgaaatggcaagcatg420
gcttctccatcgattaacaagcctgacctgagcttttag459
<210>10
<211>152
<212>PRT
<213>sweetcorn（ZmOXSL1AT3）
<400>10
LeuSerProValAsnThrAsnArgMetTyrSerProArgGlyLeuAsp
151015
ProSerIleIleHisSerProPheGlyGlyMetSerProArgSerPro
202530
ArgThrMetGluLeuThrSerProLeuSerValArgValGlyValGly
354045
AlaAlaValThrProArgAspMetLeuAspGlnPheSerSerLeuAsn
505560
LysHisGlnValProSerValGlySerProArgAsnLeuAsnAlaSer
65707580
TrpGlyAsnIleGlyThrProLysSerLysValAspTrpGlyValAsp
859095
AspAspGluLeuValArgLeuArgHisProValGlnHisGlyAsnThr
100105110
GluGluGluProAspValSerTrpValGlnSerLeuValAsnHisAla
115120125
GluLeuAsnGlyLysArgGlyGluMetAlaSerMetAlaSerProSer
130135140
IleAsnLysProAspLeuSerPhe
145150
<210>11
<211>18
<212>DNA
<213>人工序列
<400>11
atgggggaagcctccgac18
<210>12
<211>25
<212>DNA
<213>人工序列
<400>12
ctagactaccatctcatccagctgg25
<210>13
<211>20
<212>DNA
<213>人工序列
<400>13
atgggcgaccttgctgatct20
<210>14
<211>25
<212>DNA
<213>人工序列
<400>14
ctaaaagctcaggtcaggcttgtta25
Claims (10)
1.玉米OXS2蛋白，其含有如下（1）-（4）任一项所示的氨基酸序列：
（1）SEQIDNO.2所示的氨基酸序列；
（2）SEQIDNO.4所示的氨基酸序列；
（3）SEQIDNO.6所示的氨基酸序列；
（4）SEQIDNO.2或SEQIDNO.4或SEQIDNO.6所示的氨基酸序列经取代、缺失和/或增加一个或多个氨基酸和/或末端修饰后且具有提高植物抗逆功能的序列。
2.编码权利要求1所述玉米OXS2蛋白的基因。
3.玉米OXS2蛋白AT3片段，其含有如下（1）-（3）任一项所示的氨基酸序列：
（1）SEQIDNO.7所示的氨基酸序列；
（2）SEQIDNO.9所示的氨基酸序列；
（3）SEQIDNO.7或SEQIDNO.9所示的氨基酸序列经取代、缺失和/或增加一个或多个氨基酸和/或末端修饰后且具有提高植物抗逆功能的序列。
4.编码权利要求3所述AT3片段的基因。
5.含有权利要求2或4所述基因序列的重组载体、重组菌或转基因细胞系、转基因植物株系。
6.玉米OXS2蛋白、其编码基因或AT3片段、其编码基因在培育抗逆植物中的应用，其中，所述玉米OXS2蛋白如权利要求1所示，所述AT3片段如权利要求3所示。
7.玉米OXS2蛋白、其编码基因或AT3片段、其编码基因在抗逆植物辅助育种中的应用，其中，所述玉米OXS2蛋白如权利要求1所示，所述AT3片段如权利要求3所示。
8.根据权利要求6或7所述的应用，其特征在于，所述抗逆性状包括抗重金属污染，抗氧化胁迫、抗高温胁迫、抗低温胁迫、抗盐碱、抗旱、抗病虫害中的至少一种。
9.根据权利要求8所述的应用，其特征在于，所述重金属包括镉、汞、金、银、铜、铁、铅、砷、铬中的至少一种。
10.一种抗逆植物的培育方法，包括将权利要求2或4所述的基因转入受体植物中，得到转基因植物；所述转基因植物与受体植物相比，其对逆境的抗性提高。
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| CN108864264A (zh) * | 2017-05-11 | 2018-11-23 | 中国科学院华南植物园 | 玉米OXS2a基因、其编码蛋白及应用 |
| CN111893134A (zh) * | 2020-07-30 | 2020-11-06 | 中国科学院华南植物园 | Oxs2基因及其编码蛋白在提高植物抗盐胁迫性中的应用 |
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| CN102229937A (zh) * | 2011-06-07 | 2011-11-02 | 河南农业大学 | 玉米bHLH类转录因子基因ZmMIT1及其编码蛋白的应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108864264A (zh) * | 2017-05-11 | 2018-11-23 | 中国科学院华南植物园 | 玉米OXS2a基因、其编码蛋白及应用 |
| CN108864264B (zh) * | 2017-05-11 | 2020-12-29 | 中国科学院华南植物园 | 玉米OXS2a基因、其编码蛋白及应用 |
| CN111893134A (zh) * | 2020-07-30 | 2020-11-06 | 中国科学院华南植物园 | Oxs2基因及其编码蛋白在提高植物抗盐胁迫性中的应用 |
| CN111893134B (zh) * | 2020-07-30 | 2022-07-19 | 中国科学院华南植物园 | Oxs2基因及其编码蛋白在提高植物抗盐胁迫性中的应用 |
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