CN105027978A

CN105027978A - Mushroom cultivation method

Info

Publication number: CN105027978A
Application number: CN201510461970.6A
Authority: CN
Inventors: 李睿坚
Original assignee: Chengdu Shengling Biotechnology Co Ltd
Current assignee: Chengdu Shengling Biotechnology Co Ltd
Priority date: 2015-07-31
Filing date: 2015-07-31
Publication date: 2015-11-11

Abstract

The invention discloses a mushroom cultivation method, and the method comprises the steps of mixing 30-60% wood chip, 20-40% secondary chopped straw hydrolyzed by cellulose, 10%-15% secondary wood chip hydrolyzed by laccase, 5%-10% white sugar and 1%-5% gesso to get the cultivation matrix; performing mushroom inoculation in the cultivation matrix for growing to get the mushroom that has large petal and small straw and tastes crisp.

Description

A kind of cultivation method of mushroom

Technical field

The invention belongs to mushroom cultivation field.

Background technology

In mushroom cultivating process, the main two problems one considered is the level of growth of mushroom itself, another is the control of miscellaneous bacteria and insect pest in mushroom cultivating process, the control of these two aspects and balance are except relying on growth conditions, as outside the regulating and control of temperature, humidity etc., also depend on the selection of culture medium, not only if culture medium is beneficial to the growth of mushroom itself but also pole is beneficial to the breeding of miscellaneous bacteria and insect, then not favourable to the cultivation of mushroom.

Summary of the invention

The object of the present invention is to provide one can both meet mushroom well to grow, obtain a mushroom mushroom that large stem stalk is little, mouthfeel is crisp and refreshing, the method for cultivating mushroom that can effectively suppress varied bacteria growing to be bred again.

Technical scheme of the present invention is as follows: a kind of cultivation method of mushroom, comprises the following steps:

1) by mass content be 20 ~ 40% crushing straw carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, all operations process is all aseptically carried out;

2) by mass content be 10 ~ 15% wood fragments bits carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, all operations process is all aseptically carried out;

3) by mass content be 30 ~ 60% wood fragments bits carry out high-temperature sterilization with the white sugar of 5% ~ 10%, the land plaster of 1% ~ 5%, consider to be worth doing with described secondary wood fragments thereafter and described secondary crushing straw mixes mutually, namely obtain culture medium, mixed process is aseptically carried out;

4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, reach about 70% to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, place under room temperature, shading, humidity are the environment of 60% ~ 70% after inoculation and within 5 ~ 10 days, carry out sending out bacterium;

5) grow under the bacterium bag after a bacterium being positioned over natural light irradiation and draughty open-air atmosphere, every other day to 1 ~ 2 moisture of sprinkling on bacterium bag, keep bacterium bag water content 60% ~ 70%, can gather after 30 ~ 60 days.

Its preferred embodiment is: described cellulase be selected from circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.

Cellulase herein also can select the complex class cellulase of commercial type.

Its another preferred embodiment is: described crushing straw is the crushed material of corn or wheat stalk.

Its another preferred embodiment is: described wood fragments bits are the limb crushed material of fruits trees.

The trees of growth fruit that refer to of said fruits trees herein, as pear tree, Japanese plum, peach, apple tree, apricot etc.

Usefulness of the present invention is:

1) Appropriate application waste straw, is beneficial to circulation and the regeneration of resource;

2) cultivate that the mushroom mushroom that obtains is piece large and stem stalk is little, mouthfeel is crisp and refreshing;

3) this cultivation method can suppress the growth of other miscellaneous bacteria and insect, obtains the mushroom of good quality.

Embodiment

Embodiment 1

1) by mass content be 15% apple tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 35 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 6h of wood fragments bits quality 10%;

2) by mass content be 20% wheat crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the compound cellulose enzyme reaction 7h of crushing straw quality 8%;

3) by mass content be 50% apple tree wood fragments bits, mass content be 10% white sugar, mass content be 5% land plaster carry out high-temperature sterilization, thereafter consider to be worth doing with above-mentioned secondary wood fragments and fully mix with secondary crushing straw, namely obtain culture medium, this process is aseptically carried out;

4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, reach about 70% to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, place under room temperature, shading, humidity are the environment of 60% ~ 70% after inoculation and within 5 days, carry out sending out bacterium;

5) grow under the bacterium bag after a bacterium being positioned over natural light irradiation and draughty open-air atmosphere, every other day to 1 moisture of sprinkling on bacterium bag, keep bacterium bag water content 60% ~ 70%, gather after 60 days.

Embodiment 2

1) by mass content be 15% pear tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 5, at 40 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 6h of wood fragments bits quality 6%;

2) by mass content be 35% corn crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 4, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the circumscribed beta glucan enzyme reaction 8h of crushing straw quality 7%;

3) by mass content be 40% pear tree wood fragments bits, mass content be 5% white sugar, mass content be 5% land plaster carry out high-temperature sterilization, thereafter to consider to be worth doing with above-mentioned secondary wood fragments and secondary crushing straw fully mixes, namely obtain culture medium, said process aseptically carries out;

4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, reach about 70% to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, place under room temperature, shading, humidity are the environment of 60% ~ 70% after inoculation and within 10 days, carry out sending out bacterium;

5) grow under the bacterium bag after a bacterium being positioned over natural light irradiation and draughty open-air atmosphere, every other day to 2 moisture of sprinkling on bacterium bag, keep bacterium bag water content 60% ~ 70%, namely gather after 40 days.

Claims

1. a cultivation method for mushroom, is characterized in that: comprise the following steps:

2. the cultivation method of mushroom according to claim 1, is characterized in that: described cellulase be selected from circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.

3. the cultivation method of mushroom according to claim 1, is characterized in that: described crushing straw is the crushed material of corn or wheat stalk.

4. the cultivation method of mushroom according to claim 1, is characterized in that: described wood fragments bits are the limb crushed material of fruits trees.