CN105027980A - Hericium erinaceus cultivation method - Google Patents
Hericium erinaceus cultivation method Download PDFInfo
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- CN105027980A CN105027980A CN201510462049.3A CN201510462049A CN105027980A CN 105027980 A CN105027980 A CN 105027980A CN 201510462049 A CN201510462049 A CN 201510462049A CN 105027980 A CN105027980 A CN 105027980A
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- hericium erinaceus
- crushing straw
- cultivation method
- temperature sterilization
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a Hericium erinaceus cultivation method, and the method comprises the steps of mixing secondary wood chip 30-50% in quality content and hydrolyzed by laccase, chopped straw 20-40% in quality content, 5%-10% secondary chopped straw hydrolyzed by cellulose, 1%-5% calcium superphosphate and 1%-5% gesso to get the cultivation matrix; then performing Hericium erinaceus inoculation in the cultivation matrix for growing to get the Hericium erinaceus that tastes crisp by utilizing the waste straw.
Description
Technical field
The invention belongs to mushroom cultivation field.
Background technology
In mushroom cultivating process, the main two problems one considered is the level of growth of mushroom itself, another is the control of miscellaneous bacteria and insect pest in mushroom cultivating process, the control of these two aspects and balance are except relying on growth conditions, as outside the regulating and control of temperature, humidity etc., also depend on the selection of culture medium, not only if culture medium is beneficial to the growth of mushroom itself but also pole is beneficial to the breeding of miscellaneous bacteria and insect, then not favourable to the cultivation of mushroom.
Summary of the invention
The object of the present invention is to provide one can both meet Hericium erinaceus well to grow, obtain the Hericium erinaceus that mouthfeel is crisp and refreshing, the Hericium erinaceus culture method that can effectively suppress varied bacteria growing to be bred again.
Technical scheme of the present invention is as follows: the cultivation method of a kind of Hericium erinaceus, comprises the following steps:
1) by mass content be 5 ~ 10% crushing straw carry out high-temperature sterilization, thereafter use acetum regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, all operations process is all aseptically carried out;
2) by mass content be 30 ~ 50% wood fragments bits carry out high-temperature sterilization, thereafter use acetum regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, all operations process is all aseptically carried out;
3) be that the crushing straw of 20 ~ 40% and superphosphate, the white sugar of 1% ~ 5%, the land plaster of 1% ~ 5% of 1% ~ 5% carry out high-temperature sterilization by mass content, thereafter to consider to be worth doing with described secondary wood fragments and described secondary crushing straw mixes mutually, namely obtain culture medium, mixed process is aseptically carried out;
4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, about 70% is reached to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, after inoculation in lower than 25 DEG C, shading, carry out under dry environment sending out bacterium and a growth, every other day moisture is sprayed to bacterium bag in this process, keep bacterium bag water content to be 60% ~ 70%, ventilate every day 1 ~ 2 time, can gather after 10 ~ 15 days.
Its preferred embodiment is: described cellulase be selected from circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
Cellulase herein also can select the complex class cellulase of commercial type.
Its another preferred embodiment is: described crushing straw is the crushed material of corn or wheat stalk.Its another preferred embodiment is: described wood fragments bits are the limb crushed material of fruits trees.
The trees of growth fruit that refer to of said fruits trees herein, as pear tree, Japanese plum, peach, apple tree, apricot etc.
Usefulness of the present invention is:
1) Appropriate application waste straw, is beneficial to circulation and the regeneration of resource;
2) this cultivation method can obtain the crisp and refreshing Hericium erinaceus of mouthfeel;
3) this cultivation method can suppress the growth of other miscellaneous bacteria and insect, obtains the Hericium erinaceus of good quality.
Embodiment
Embodiment 1
1) by mass content be 50% apple tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 4, at 40 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 12h of wood fragments bits quality 10%;
2) by mass content be 10% wheat crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the compound cellulose enzyme reaction 8h of crushing straw quality 8%;
3) by mass content be 30% wheat crushing straw, the mass content superphosphate that is 1%, the mass content white sugar that is 4%, mass content be 5% land plaster carry out high-temperature sterilization, thereafter to consider to be worth doing with above-mentioned secondary wood fragments and secondary crushing straw fully mixes, namely obtain culture medium, said process aseptically carries out;
4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, about 70% is reached to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, after inoculation in 20 DEG C, shading, carry out under dry environment sending out bacterium and a growth, every other day moisture is sprayed to bacterium bag in this process, keep bacterium bag water content to be 70%, ventilate every day 2 times, gather after 10 days.
Embodiment 2
1) by mass content be 35% pear tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 35 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 8h of wood fragments bits quality 10%;
2) by mass content be 10% corn crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 5, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the circumscribed beta glucan enzyme reaction 6h of crushing straw quality 10%;
3) by mass content be 40% corn crushing straw, the mass content superphosphate that is 5%, the mass content white sugar that is 5%, mass content be 5% land plaster carry out high-temperature sterilization, thereafter to consider to be worth doing with above-mentioned secondary wood fragments and secondary crushing straw fully mixes, namely obtain culture medium, said process aseptically carries out;
4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, about 70% is reached to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, after inoculation in 20 DEG C, shading, carry out under dry environment sending out bacterium and a growth, every other day moisture is sprayed to bacterium bag in this process, keep bacterium bag water content to be 65%, ventilate every day 1 time, gather after 15 days.
Claims (4)
1. a cultivation method for Hericium erinaceus, is characterized in that: comprise the following steps:
1) by mass content be 5 ~ 10% crushing straw carry out high-temperature sterilization, thereafter use acetum regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, all operations process is all aseptically carried out;
2) by mass content be 30 ~ 50% wood fragments bits carry out high-temperature sterilization, thereafter use acetum regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, all operations process is all aseptically carried out;
3) be that the crushing straw of 20 ~ 40% and superphosphate, the white sugar of 1% ~ 5%, the land plaster of 1% ~ 5% of 1% ~ 5% carry out high-temperature sterilization by mass content, thereafter to consider to be worth doing with described secondary wood fragments and described secondary crushing straw mixes mutually, namely obtain culture medium, mixed process is aseptically carried out;
4) culture medium is aseptically packed, and the water sprayed in bag through high-temperature sterilization, about 70% is reached to water content of substrate, obtain bacterium bag, inoculate in bacterium bag, after inoculation in lower than 25 DEG C, shading, carry out under dry environment sending out bacterium and a growth, every other day moisture is sprayed to bacterium bag in this process, keep bacterium bag water content to be 60% ~ 70%, ventilate every day 1 ~ 2 time, can gather after 10 ~ 15 days.
2. the cultivation method of Hericium erinaceus according to claim 1, is characterized in that: described cellulase be selected from circumscribed 1,4 beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
3. the cultivation method of Hericium erinaceus according to claim 1, is characterized in that: described crushing straw is the crushed material of corn or wheat stalk.
4. the cultivation method of Hericium erinaceus according to claim 1, is characterized in that: described wood fragments bits are the limb crushed material of fruits trees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510462049.3A CN105027980A (en) | 2015-07-31 | 2015-07-31 | Hericium erinaceus cultivation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510462049.3A CN105027980A (en) | 2015-07-31 | 2015-07-31 | Hericium erinaceus cultivation method |
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| Publication Number | Publication Date |
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| CN105027980A true CN105027980A (en) | 2015-11-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510462049.3A Pending CN105027980A (en) | 2015-07-31 | 2015-07-31 | Hericium erinaceus cultivation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667778A (en) * | 2017-10-22 | 2018-02-09 | 长沙爱扬医药科技有限公司 | Utilize the method for Yellow Fructus Gardeniae discarded object cultivation Hericium erinaceus |
| CN111690544A (en) * | 2020-07-10 | 2020-09-22 | 山东省科学院生物研究所 | Oyster mushroom capable of producing laccase and application thereof |
Citations (5)
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|---|---|---|---|---|
| CN102329171A (en) * | 2011-07-15 | 2012-01-25 | 安徽省庆元堂徽菊有限公司 | Culture base material for cultivating shiitake by using chrysanthemum straws and method for cultivating shiitake by using culture base material |
| CN103601553A (en) * | 2013-11-25 | 2014-02-26 | 青岛茂丰有机蔬菜有限公司 | Method for using iron-enriched hericium erinaceus culture medium |
| CN104429607A (en) * | 2014-12-09 | 2015-03-25 | 张鹏 | Application method for zinc-rich hericium erinaceus culture medium |
| CN104429621A (en) * | 2014-12-25 | 2015-03-25 | 皖南大鹏天然产物有限公司 | Production method for extracting hericium erinaceus mycelia of hericium erinaceus polysaccharide |
| CN104541972A (en) * | 2014-12-31 | 2015-04-29 | 安徽丰原发酵技术工程研究有限公司 | Method for cultivating edible fungi through agricultural straws |
-
2015
- 2015-07-31 CN CN201510462049.3A patent/CN105027980A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329171A (en) * | 2011-07-15 | 2012-01-25 | 安徽省庆元堂徽菊有限公司 | Culture base material for cultivating shiitake by using chrysanthemum straws and method for cultivating shiitake by using culture base material |
| CN103601553A (en) * | 2013-11-25 | 2014-02-26 | 青岛茂丰有机蔬菜有限公司 | Method for using iron-enriched hericium erinaceus culture medium |
| CN104429607A (en) * | 2014-12-09 | 2015-03-25 | 张鹏 | Application method for zinc-rich hericium erinaceus culture medium |
| CN104429621A (en) * | 2014-12-25 | 2015-03-25 | 皖南大鹏天然产物有限公司 | Production method for extracting hericium erinaceus mycelia of hericium erinaceus polysaccharide |
| CN104541972A (en) * | 2014-12-31 | 2015-04-29 | 安徽丰原发酵技术工程研究有限公司 | Method for cultivating edible fungi through agricultural straws |
Non-Patent Citations (1)
| Title |
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| 周光龙: "漆酶制剂及其在食用菌生产中的应用", 《食用菌》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107667778A (en) * | 2017-10-22 | 2018-02-09 | 长沙爱扬医药科技有限公司 | Utilize the method for Yellow Fructus Gardeniae discarded object cultivation Hericium erinaceus |
| CN111690544A (en) * | 2020-07-10 | 2020-09-22 | 山东省科学院生物研究所 | Oyster mushroom capable of producing laccase and application thereof |
| CN111690544B (en) * | 2020-07-10 | 2021-12-07 | 山东省科学院生物研究所 | Oyster mushroom capable of producing laccase and application thereof |
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