CN105036946A - Preparation method of culture substrate of coprinus comatus - Google Patents

Preparation method of culture substrate of coprinus comatus Download PDF

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Publication number
CN105036946A
CN105036946A CN201510461854.4A CN201510461854A CN105036946A CN 105036946 A CN105036946 A CN 105036946A CN 201510461854 A CN201510461854 A CN 201510461854A CN 105036946 A CN105036946 A CN 105036946A
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CN
China
Prior art keywords
preparation
culture medium
coprinus comatus
wood fragments
crushing straw
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510461854.4A
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Chinese (zh)
Inventor
李睿坚
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chengdu Shengling Biotechnology Co Ltd
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Chengdu Shengling Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Chengdu Shengling Biotechnology Co Ltd filed Critical Chengdu Shengling Biotechnology Co Ltd
Priority to CN201510461854.4A priority Critical patent/CN105036946A/en
Publication of CN105036946A publication Critical patent/CN105036946A/en
Pending legal-status Critical Current

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  • Processing Of Solid Wastes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a preparation method of a culture substrate of coprinus comatus. The culture substrate is prepared by mixing 30-60% of smashed wood chips, 20-40% of secondary smashed straw hydrolyzed by cellulase, 10-15% of secondary smashed wood chip hydrolyzed by laccase, 5-10% of urea and 1-5% of gypsum powder. According to the preparation method of the culture substrate of coprinus comatus, provided by the invention, straw waste is fully utilized, and the cultured coprinus comatus is crisp and refreshing in taste.

Description

The preparation method of the culture medium of a kind of Coprinus comatus
Technical field
The invention belongs to mushroom cultivation field.
Background technology
In mushroom cultivating process, the main two problems one considered is the level of growth of mushroom itself, another is the control of miscellaneous bacteria and insect pest in mushroom cultivating process, the control of these two aspects and balance are except relying on growth conditions, as outside the regulating and control of temperature, humidity etc., also depend on the selection of culture medium, not only if culture medium is beneficial to the growth of mushroom itself but also pole is beneficial to the breeding of miscellaneous bacteria and insect, then not favourable to the cultivation of mushroom.
Summary of the invention
The object of the present invention is to provide one can both meet Coprinus comatus well to grow, obtain the Coprinus comatus that mouthfeel is crisp and refreshing, the preparation method of the culture medium that can effectively suppress varied bacteria growing to be bred again.
Technical scheme of the present invention is as follows: the preparation method of the culture medium of a kind of Coprinus comatus, comprises the following steps:
1) by mass content be 20 ~ 40% crushing straw carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, all operations process is all aseptically carried out;
2) by mass content be 10 ~ 15% wood fragments bits carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, all operations process is all aseptically carried out;
3) by mass content be 30 ~ 60% wood fragments bits with 1% ~ 5% terra alba carry out high-temperature sterilization, consider to be worth doing with described secondary wood fragments thereafter, described secondary crushing straw and 5% ~ 10% urea mix mutually, namely obtain culture medium, mixing process is aseptically carried out.
Its preferred embodiment is: described cellulase be selected from circumscribed beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
Cellulase herein also can select the complex class cellulase of commercial type.
Its another preferred embodiment is: described crushing straw is the crushed material of corn or wheat stalk.Its another preferred embodiment is: described wood fragments bits are the limb crushed material of fruits trees.
The trees of growth fruit that refer to of said fruits trees herein, as pear tree, Japanese plum, peach, apple tree, apricot etc.
Usefulness of the present invention is:
1) Appropriate application waste straw, is beneficial to circulation and the regeneration of resource;
2) culture medium prepared can grow and obtain the crisp and refreshing Coprinus comatus of mouthfeel;
3) this culture medium can suppress the growth of other miscellaneous bacteria and insect, obtains the Coprinus comatus of good quality.
Embodiment
Embodiment 1
1) by mass content be 15% apple tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 35 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 10h of wood fragments bits quality 8%;
2) by mass content be 20% wheat crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 5, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the compound cellulose enzyme reaction 8h of crushing straw quality 10%;
3) by mass content be 50% apple tree wood fragments bits, mass content be 5% terra alba carry out high-temperature sterilization, be the urea of 10% with mass content thereafter, and above-mentioned secondary wood fragments bits fully mix with secondary crushing straw, namely obtain culture medium;
Said process all aseptically carries out.
Embodiment 2
1) by mass content be 15% pear tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 35 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 6h of wood fragments bits quality 6%;
2) by mass content be 35% corn crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 5, at 40 DEG C, add thereafter quality is obtain secondary crushing straw after the circumscribed beta-glucan enzyme reaction 10h of crushing straw quality 8%;
3) by mass content be 40% pear tree wood fragments bits, mass content be 5% terra alba carry out high-temperature sterilization, consider to be worth doing with above-mentioned secondary wood fragments thereafter with secondary crushing straw, and mass content be 5% urea fully mix, namely obtain culture medium, said process aseptically carries out.

Claims (4)

1. a preparation method for the culture medium of Coprinus comatus, is characterized in that: comprise the following steps:
1) by mass content be 20 ~ 40% crushing straw carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, all operations process is all aseptically carried out;
2) by mass content be 10 ~ 15% wood fragments bits carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, all operations process is all aseptically carried out;
3) by mass content be 30 ~ 60% wood fragments bits with 1% ~ 5% terra alba carry out high-temperature sterilization, consider to be worth doing with described secondary wood fragments thereafter, described secondary crushing straw and 5% ~ 10% urea mix mutually, namely obtain culture medium, mixing process is aseptically carried out.
2. the preparation method of the culture medium of Coprinus comatus according to claim 1, is characterized in that: described cellulase be selected from circumscribed beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
3. the preparation method of the culture medium of Coprinus comatus according to claim 1, is characterized in that: described crushing straw is the crushed material of corn or wheat stalk.
4. the preparation method of the culture medium of Coprinus comatus according to claim 1, is characterized in that: described wood fragments bits are the limb crushed material of fruits trees.
CN201510461854.4A 2015-07-31 2015-07-31 Preparation method of culture substrate of coprinus comatus Pending CN105036946A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510461854.4A CN105036946A (en) 2015-07-31 2015-07-31 Preparation method of culture substrate of coprinus comatus

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Application Number Priority Date Filing Date Title
CN201510461854.4A CN105036946A (en) 2015-07-31 2015-07-31 Preparation method of culture substrate of coprinus comatus

Publications (1)

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CN105036946A true CN105036946A (en) 2015-11-11

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106542895A (en) * 2016-10-31 2017-03-29 成都天绿菌业有限公司 A kind of culture medium for cultivating chicken leg mushroom

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101974436A (en) * 2010-09-21 2011-02-16 中国农业科学院农业资源与农业区划研究所 Lignocellulose degrading bacteria and application thereof
CN103497018A (en) * 2013-08-29 2014-01-08 合肥市潜溪山庄农业生态园有限公司 Needle mushroom cultivation matrix formula and its preparation method
CN104541972A (en) * 2014-12-31 2015-04-29 安徽丰原发酵技术工程研究有限公司 Method for cultivating edible fungi through agricultural straws

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101974436A (en) * 2010-09-21 2011-02-16 中国农业科学院农业资源与农业区划研究所 Lignocellulose degrading bacteria and application thereof
CN103497018A (en) * 2013-08-29 2014-01-08 合肥市潜溪山庄农业生态园有限公司 Needle mushroom cultivation matrix formula and its preparation method
CN104541972A (en) * 2014-12-31 2015-04-29 安徽丰原发酵技术工程研究有限公司 Method for cultivating edible fungi through agricultural straws

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
王贺祥,刘庆洪主编: "《食用菌栽培手册》", 31 January 2015 *
申进文编著: "《食用菌生产技术大全》", 31 January 2014 *
赵金海主编: "《生物化学》", 31 January 2013 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106542895A (en) * 2016-10-31 2017-03-29 成都天绿菌业有限公司 A kind of culture medium for cultivating chicken leg mushroom

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