CN105010750B - A kind of method for producing epsilon-polylysine and protein feed simultaneously using corn's by-products - Google Patents
A kind of method for producing epsilon-polylysine and protein feed simultaneously using corn's by-products Download PDFInfo
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- CN105010750B CN105010750B CN201510417599.3A CN201510417599A CN105010750B CN 105010750 B CN105010750 B CN 105010750B CN 201510417599 A CN201510417599 A CN 201510417599A CN 105010750 B CN105010750 B CN 105010750B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of methods for producing ε polylysines and protein feed simultaneously using corn's by-products.Maize peel and the plumule dregs of rice are handled respectively with amylase and cellulase, starch therein and few fibers matter are hydrolyzed into reduced sugar, filtrate after separation of solid and liquid is mixed with glucose mother liquid as carbon source, and cooperation corn pulp carries out liquid state fermentation production ε polylysines as organic nitrogen source；Filter residue after separation of solid and liquid can obtain the maize peel containing protein 12~20% and the plumule dregs of rice containing protein 25~35% after drying, and the protein content than conventional maize peel and the plumule dregs of rice is high, thus improves the added value of maize peel and the plumule dregs of rice.
Description
Technical field
The technical field that the invention belongs to ferment with Feed Manufacturing is specifically related to a kind of using corn's by-products while raw
The method for producing epsilon-polylysine and protein feed.
Background technology
Corn starch-containing 70% or so, fatty 4~5%.In corn deep processing industry, principal product starch, starch are removed
Sugar and corn oil, by-product account for the 25~30% of corn ingredient.Specific by-product species have maize peel, the plumule dregs of rice, corn pulp,
Albumen powder, glucose mother liquid etc..Various corn's by-products are sold separately generally as feedstuff, and profit is very low.It is such as beautiful
Rice ＆ peanut milk, although protein content is high, due to the presence of sulphur, the grey ingredient that grades, the palatability as feedstuff is poor, soluble egg
White and part sugar presence makes its not easy to be processed into solid material, and sale radius is small, so selling price is very low, becomes very much
The burden of corn processing enterprise.Glucose mother liquid is fitted on crystallizing tank by repeatedly returning, and causes since concentration of oligosaccharide gradually rises
Crystallization is difficult and is exited production system, is made inquiries even if underselling still to rarely have as a kind of sugar source, and occupy a large amount of
Storage tank and the machining chain for having influenced enterprise, the problem of also becoming enterprise's headache.Maize peel and the plumule dregs of rice for another example, due to fibre
It is high to tie up matter content, protein content is low so that its animal digestion rate is low, and inherently a kind of feedstuff of low price, there is also pins
Sell the problem of pressure is big.As corn processing industry and feedstuff industry are increasingly competitive, by-product selling pressure how is solved
And raising added value becomes the task of top priority of enterprise.
Epsilon-polylysine is a kind of novel Natural anti-mildew preservative, is that one kind is similar to antibiotic but is not belonging to antibiotic
Substance, it is not only harmful to human health, and is the direction of Future Development also with certain nutritive value.Epsilon-polylysine
With broad spectrum antibacterial, there is good fungistatic effect to gram-positive bacteria, Gram-negative bacteria, saccharomycete, mould, be mesh
There is the microbiology class preservative of superior antiseptic property and huge business potential in preceding natural antiseptic agent.On April 3rd, 2014, state
Hygienic State Family Planning Commission of family has approved application of the epsilon-polylysine as food additives new varieties.Epsilon-polylysine can pass through liquid depth
Layer fermenting and producing, there are many carbon source kind, there is glucose, fructose, starch, glycerine, xylose, arabinose etc..Organic nitrogen source can be with
With beef extract, peptone, fish meal, yeast extract, corn pulp etc..In fermentation industry, the selection of carbon source and nitrogen source often will
Consider raw material sources and price.
Invention content
A kind of utilization corn by-product is provided it is an object of the invention to be directed to the problem of corn's by-products utilization rate is extremely low
The method that object produces epsilon-polylysine and protein feed simultaneously, this method is by maize peel, the plumule dregs of rice, corn pulp, glucose mother liquid
It is integrated, carbon source and nitrogen source in by-product can be efficiently used and is promoted the protein content of maize peel and the plumule dregs of rice,
It can solve the problems, such as that corn's by-products provide an effective approach for enterprise with large-scale production and application.
The technical scheme is that：A kind of side for producing epsilon-polylysine and protein feed simultaneously using corn's by-products
Method includes the following steps：
（1）Add water stirring in maize peel and the plumule dregs of rice respectively, its water content is made to be all higher than 60%；
（2）Respectively in step（1）Added in each material base of maize peel and the plumule dregs of rice of gained amylase and cellulase into
The mass ratio of row enzymolysis, amylase and cellulase is 1:1~1:3, and enzyme total amount is not less than each material base weight amount
0.1%；Ranging from 30~70 DEG C of hydrolysis temperature, processing time is at least 15 h；
（3）After enzymolysis, maize peel is digested into mother liquor respectively and plumule dregs of rice enzymolysis mother liquor carries out separation of solid and liquid, respectively
Obtain maize peel enzymolysis filtrate I, maize peel enzymolysis filter residue I, plumule dregs of rice enzymolysis filtrate I and plumule dregs of rice enzymolysis filter residue I；With quality two
The water washing maize peel enzymolysis filter residue I of times maize peel enzymolysis filtrate I, with the washing of the twice plumule dregs of rice enzymolysis filtrate I of quality
Wash plumule dregs of rice enzymolysis filter residue I；Separation of solid and liquid is carried out again obtains maize peel enzymolysis filtrate II, maize peel enzymolysis filter residue II, plumule
The dregs of rice digest filtrate II and plumule dregs of rice enzymolysis filter residue II；
（4）Step（3）Each total filter residue of middle gained maize peel and the plumule dregs of rice, water content is 55~60%, respectively with drying
After equipment drying, protein feed maize peel and the plumule dregs of rice are obtained；
（5）By step（3）Middle gained whole filtrate I mixes, then mixed with glucose mother liquid with whole filtrates II, the part
Syrup is as fermenting carbon source；The another corn pulp taken in corn's by-products is as fermentation organic nitrogen source；
（6）The fermentation medium components of epsilon-polylysine are：Syrup, corn pulp, (NH4)2SO4And MgSO4；
（7）According to conventional epsilon-polylysine zymotechnique, step is used（6）The culture medium fermentation production of epsilon-poly- bad ammonia
Acid.
The step（6）In syrup include filtrate I, filtrate II and glucose mother liquid.
The step（7）The temperature of middle fermentation is 25~40 DEG C.
The step（7）Epsilon-polylysine producing strains used in middle fermentation are streptomyces albusS.albulus, streptomyces lydicusS.lydicus, kitasatosporiaKitasatospora.sp, the orange streptomycete of ashS.griseoaurantiacus, the chromogenic chain of amylase
MouldS.diastatochromogenes, bacillus subtilisBacillus subtilis, streptomyces graminearusS.graminearus, streptomyces hygroscopicusS.hygroscopicus, the brown streptomycete of ashS.griseofuscus, european bird cherry streptomyceteS.padanusAt least one of.
Beneficial effects of the present invention are：The method of the invention amylase and cellulase processing maize peel and plumule
The dregs of rice digest starch therein and few fibers matter into reduced sugar.Maize peel and the plumule dregs of rice carry out separation of solid and liquid after enzymolysis,
Filtrate produces epsilon-polylysine as carbon source through fermentation, and protein feed is used as after filter residue drying.The protein content of ordinary maize skin is
7-10%, conventional plumule cake protein content be 16-18%, and the cornbran protein content obtained in the present invention be 12-20%, plumule
Cake protein content is 25-35%.The method of the invention has the following advantages：
1st, production cost is low, small investment, quick, high efficiency.
2nd, enzymolysis and fermentation condition are extensive, and the period is short, and production efficiency is high.
3rd, in corn's by-products maize peel, the plumule dregs of rice, glucose mother liquid, corn pulp effectively integrate, Ji Nengfa
Ferment produces epsilon-polylysine, and improves the added value of maize peel and the plumule dregs of rice.
The present invention is related to for the first time with corn's by-products fermentation production of epsilon-polylysine and promotes maize peel and the plumule dregs of rice
Added value handles by-product for corn deep processing industry and provides an effective solution route, has great economic benefit
And social benefit.
Specific embodiment
It is embodiment below, the present invention is further described, but there is no limit to the present invention.
Embodiment 1
（1）1 kg of maize peel is taken, adds in 3 kg of water, then material moisture is 75%；1 kg of the plumule dregs of rice is taken, adds in 3 kg of water,
Then material moisture is 75%；
（2）1.5 g of amylase is added in into two kinds of materials respectively, 1.5 g of cellulase, i.e. enzyme concentration are material butt
0.15%。
After stirring evenly, material is put into 50 DEG C of baking oven, places 24 h；
（3）The material of maize peel and the plumule dregs of rice is taken out, respectively with three layers of gauze wrapped, firmly squeezes and carries out solid-liquid point
From obtaining
Obtain filtrate and filter residue.Maize peel material can obtain 1.5 kg of filtrate, 2.5 kg of filter residue；Plumule dregs of rice material can be filtered
1.8 kg of liquid, 2.2 kg of filter residue；
（4）3 kg water are added in into maize peel filter residue, after mixing after squeezed again with gauze, obtain filtrate 3.2 kg
,
2.3 kg of filter residue；3 kg water are added in into plumule dregs of rice filter residue, are squeezed again with gauze after mixing, obtain filtrate
3.2 kg, 2 kg of filter residue；
（5）The plumule dregs of rice filter residue of the maize peel filter residue of 2.3 kg and 2 kg are put into baking oven respectively, after 80 DEG C of drying,
Obtain 0.7 kg of maize peel, protein content 14%；Obtain 0.55 kg of the plumule dregs of rice, protein content 30%.
Slant medium：Glucose 1.0%, corn pulp 1.0%, agar 2.0%, 7.5,115 DEG C of 20 min of sterilizing of pH.
Seed culture medium：Glucose 2.0%, corn pulp 1.0%, magnesium sulfate 0.1%, 7.5,115 DEG C of 20 min of sterilizing of pH.
In this embodiment, first time separation of solid and liquid obtains maize peel filtrate 1.5 kg, 1.8 kg of plumule dregs of rice filtrate；Second
Secondary separation of solid and liquid obtains maize peel filtrate 3.2 kg, plumule dregs of rice filtrate 3.2kg.These filtrates are mixed, totally 9.7 kg.
Fermentation medium：10.3 kg of filtrate, 1 kg of glucose mother liquid, corn pulp 0.5 kg, (NH4)2SO4100 g,
MgSO410 g, 115 DEG C of 20 min of sterilizing.
By streptomyces albusS. albulusSpore access slant medium, 30 DEG C cultivate 7 days, obtain strain inclined plane；
Then a ring slant strains are connect and enter seed culture medium, 30 DEG C are cultivated 1 day, obtain the seed liquor of streptomyces albus.
Above-mentioned fermentation medium is packed into the fermentation tank for being 15 L in volume, 1 L streptomyces albus seed liquor is accessed and is sent out
Ferment culture medium.Fermentation temperature is 30 DEG C, and initial pH is 7.5, and ventilatory capacity is 1.0 vvm, and speed of agitator is 500 rpm.When pH from
When so dropping to 4.0, stream plus a concentration of 15% ammonium hydroxide into tank, it is 4.0 ± 0.2 to maintain zymotic fluid pH, when concentration of reduced sugar is low
Fermentation ends when 5 g/L.A concentration of 15 g/L containing epsilon-polylysine in zymotic fluid at this time.
Embodiment 2
（1）1 kg of maize peel is taken, adds in 3 kg of water, then material moisture is 75%；1 kg of the plumule dregs of rice is taken, adds in 3 kg of water,
Then material moisture is 75%.
（2）3 g of amylase is added in into two kinds of materials respectively, 3 g of cellulase, i.e. enzyme concentration are material butt
0.3%.After stirring evenly, material is put into 50 DEG C of baking oven, places 24 h.
（3）Maize peel and the plumule dregs of rice are taken out, respectively with three layers of gauze wrapped, firmly squeezes and tightens, obtain filtrate and filter
Slag.Maize peel material can obtain 1.7 kg of filtrate, 2.3 kg of filter residue；Plumule dregs of rice material can obtain 2 kg of filtrate, 2 kg of filter residue.
（4）3 kg water are added in into maize peel filter residue, are squeezed again with gauze after mixing, obtain 2 kg of filter residue, filtrate 3.3
kg；3 kg water are added in into plumule dregs of rice filter residue, are squeezed again with gauze after mixing, obtain 1.7 kg of filter residue, 3.3 kg of filtrate.
（5）The plumule dregs of rice filter residue of the maize peel filter residue of 2 kg and 1.7 kg are put into baking oven respectively, 80 DEG C of drying obtain
Obtain 0.55 kg of maize peel, protein content 18%；Obtain 0.45 kg of the plumule dregs of rice, protein content 35%.
Slant medium：Glucose 1.0%, corn pulp 1.0%, agar 2.0%, 7.5,115 DEG C of 20 min of sterilizing of pH.
Seed culture medium：Glucose 2.0%, corn pulp 1.0%, magnesium sulfate 0.1%, 7.5,115 DEG C of 20 min of sterilizing of pH.
In this embodiment, first time separation of solid and liquid obtains maize peel filtrate 1.7 kg, 2 kg of plumule dregs of rice filtrate；Second
Separation of solid and liquid obtains maize peel filtrate 3.3 kg, 3.3 kg of plumule dregs of rice filtrate.These filtrates are mixed, totally 10.3 kg.
Fermentation medium：10.3 kg of filtrate, 1 kg of glucose mother liquid, corn pulp 0.5 kg, (NH4)2SO4100 g,
MgSO410 g, 115 DEG C of 20 min of sterilizing.
By streptomyces albusS. albulusSpore access slant medium, 30 DEG C cultivate 7 days, obtain strain inclined plane；
Then a ring slant strains are connect and enter seed culture medium, 30 DEG C are cultivated 1 day, obtain the seed liquor of streptomyces albus.
Above-mentioned fermentation medium is packed into the fermentation tank for being 15 L in volume, 1 L streptomyces albus seed liquor is accessed and is sent out
Ferment culture medium.Fermentation temperature is 30 DEG C, and initial pH is 7.5, and ventilatory capacity is 1.0 vvm, and speed of agitator is 500 rpm.When pH from
When so dropping to 4.0, stream plus a concentration of 15% ammonium hydroxide into tank, it is 4.0 ± 0.2 to maintain zymotic fluid pH, when concentration of reduced sugar is low
Fermentation ends when 5 g/L.A concentration of 15 g/L containing epsilon-polylysine in zymotic fluid at this time.
Claims (3)
1. a kind of method for being produced epsilon-polylysine and protein feed simultaneously using corn's by-products, is included the following steps：
（1）Add water stirring in maize peel and the plumule dregs of rice respectively, its water content is made to be all higher than 60%；
（2）Respectively in step（1）Amylase is added in each material base of maize peel and the plumule dregs of rice of gained and cellulase carries out enzyme
The mass ratio of solution, amylase and cellulase is 1:1~1:3, and enzyme total amount is not less than the 0.1% of each material base weight amount；Enzyme
It is 30~70 DEG C to solve temperature range, and processing time is at least 15 h；
（3）After enzymolysis, maize peel is digested into mother liquor respectively and plumule dregs of rice enzymolysis mother liquor carries out separation of solid and liquid, is respectively obtained
Maize peel enzymolysis filtrate I, maize peel enzymolysis filter residue I, plumule dregs of rice enzymolysis filtrate I and plumule dregs of rice enzymolysis filter residue I；It is twice with quality
The water washing maize peel enzymolysis filter residue I of maize peel enzymolysis filtrate I, with the water washing embryo of the twice plumule dregs of rice enzymolysis filtrate I of quality
The bud dregs of rice digest filter residue I；Separation of solid and liquid is carried out again obtains maize peel enzymolysis filtrate II, maize peel enzymolysis filter residue II, plumule dregs of rice enzyme
Solve filtrate II and plumule dregs of rice enzymolysis filter residue II；
（4）Step（3）Each total filter residue of middle gained maize peel and the plumule dregs of rice, water content is 55~60%, uses drying equipment respectively
After drying, protein feed maize peel and the plumule dregs of rice are obtained；
（5）By step（3）Middle gained whole filtrate I mixes, then mixed with glucose mother liquid with whole filtrates II, the part syrup
As fermenting carbon source；The another corn pulp taken in corn's by-products is as fermentation organic nitrogen source；
（6）The fermentation medium components of epsilon-polylysine are：Syrup, corn pulp, (NH4)2SO4And MgSO4；Wherein syrup includes filter
Liquid I, filtrate II and glucose mother liquid；
（7）According to conventional epsilon-polylysine zymotechnique, step is used（6）The culture medium fermentation production of epsilon-polylysine.
2. the method for producing epsilon-polylysine and protein feed simultaneously using corn's by-products according to claim 1, special
Sign is, the step（7）The temperature of middle fermentation is 25~40 DEG C.
3. the method for producing epsilon-polylysine and protein feed simultaneously using corn's by-products according to claim 1, special
Sign is, the step（7）Epsilon-polylysine producing strains used in middle fermentation are streptomyces albusS.albulus, streptomyces lydicusS.lydicus, kitasatosporiaKitasatospora.sp, the orange streptomycete of ashS.griseoaurantiacus, the chromogenic chain of amylase
MouldS.diastatochromogenes, bacillus subtilisBacillus subtilis, streptomyces graminearusS.graminearus, streptomyces hygroscopicusS.hygroscopicus, the brown streptomycete of ashS.griseofuscus, european bird cherry streptomyceteS.padanusAt least one of.
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| CN104642729A (en) * | 2015-02-09 | 2015-05-27 | 山东西王糖业有限公司 | Method for producing protein feed by using corn deep-processing by-products as raw material |
| CN110574824A (en) * | 2018-07-25 | 2019-12-17 | 广州博采生物科技有限公司 | Utilization method of vegetable oil cake meal |
| CN110200131A (en) * | 2019-07-01 | 2019-09-06 | 博益德（北京）生物科技有限公司 | A kind of single enzymatic hydrolysis mixed fermentation prepares the method and application of functional fiber feed |
| CN111534474B (en) * | 2020-03-30 | 2022-06-10 | 江南大学 | Recombinant bacillus subtilis and application thereof in production of epsilon-polylysine |
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Denomination of invention: Simultaneous production method using corn by-product e- Method for producing polylysine and protein feed Effective date of registration: 20211221 Granted publication date: 20180629 Pledgee: China Construction Bank Corporation Zouping sub branch Pledgor: SHANDONG XIWANG SUGAR INDUSTRY Co.,Ltd. Registration number: Y2021980015836 |
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