CN105010750A - Method for co-producing epsilon-polylysine and protein feed using byproducts of corns - Google Patents
Method for co-producing epsilon-polylysine and protein feed using byproducts of corns Download PDFInfo
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- CN105010750A CN105010750A CN201510417599.3A CN201510417599A CN105010750A CN 105010750 A CN105010750 A CN 105010750A CN 201510417599 A CN201510417599 A CN 201510417599A CN 105010750 A CN105010750 A CN 105010750A
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- enzymolysis
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- polylysine
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The present invention discloses a method for co-producing epsilon-polylysine and protein feed using byproducts of corns. Corn bran and germ meal are treated with amylase and cellulase respectively, which enable starch and a part of cellulose to be hydrolyzed into reducing sugars, the filtrate obtained after solid-liquid separation is mixed with glucose stock solution and the mixture is used as a carbon source, and by coordinating the carbon source with corn syrup which is used as an organic nitrogen source, epsilon-polylysine is produced by conducting liquid state fermentation; the filter residues obtained after the solid-liquid separation is dried to obtain the corn bran containing 12-20% of protein and the germ meal containing 25-35% of protein, and the protein content is higher than that of conventional corn bran and germ meal, thus the added values of corn bran and germ meal are promoted.
Description
Technical field
The invention belongs to the technical field of fermentation and Feed Manufacturing, be specifically related to a kind of method utilizing corn's by-products simultaneously to produce epsilon-polylysine and protein feed.
Background technology
Corn starch-containing about 70%, fatty 4 ~ 5%.In corn deep processing industry, removing principal product starch, starch sugar and corn oil, accessory substance account for 25 ~ 30% of corn composition.Concrete accessory substance kind has maize peel, the plumule dregs of rice, corn steep liquor, albumen powder, glucose mother liquid etc.Various corn's by-products generally sells separately as feedstuff, and profit is very low.Such as corn steep liquor, although protein content is high, because sulphur, ash grade the existence of composition, palatability as feedstuff is poor, the existence of soluble protein and part sugar makes it not easily be processed into solid material, sale radius is little, so selling price is very low, becomes the burden of a lot of corn processing enterprise.Glucose mother liquid is fitted on crystallizing tank through repeatedly returning, due to concentration of oligosaccharide raise gradually cause crystallization difficulty and exited production system, make inquiries even if undersell still to rarely have as the sugared source of one, and take a large amount of storage tank and had influence on the machining chain of enterprise, also becoming the problem of enterprise's headache.Maize peel and the plumule dregs of rice for another example, because cellulosic content is high, protein content is low, and make its animal digestion rate low, inherently a kind of feedstuff of low price, also exists the problem that selling pressure is large.Along with corn processing industry and feedstuff industry increasingly competitive, how solving accessory substance selling pressure and improving added value becomes the task of top priority of enterprise.
Epsilon-polylysine is a kind of novel Natural anti-mildew anticorrisive agent, and be that one is similar to antibiotic but does not belong to antibiotic material, it is not only harmful to human health, and also has certain nutritive value, is the direction of Future Development.Epsilon-polylysine has broad spectrum antibacterial, all has good fungistatic effect to gram-positive bacteria, Gram-negative bacteria, saccharomycete, mould, is the microbiology class anticorrisive agent in current natural antiseptic agent with superior antiseptic property and huge business potential.On April 3rd, 2014, national health State Family Planning Commission have approved the application of epsilon-polylysine as food additives new varieties.Epsilon-polylysine is produced by liquid submerged fermentation, and carbon source kind is a lot, has glucose, fructose, starch, glycerine, wood sugar, arabinose etc.Organic nitrogen source can use beef extract, peptone, fish meal, yeast extract, corn steep liquor etc.In fermentation industry, the selection of carbon source and nitrogenous source often will consider raw material sources and price.
Summary of the invention
The object of the invention is to for the extremely low problem of corn's by-products utilization rate and a kind of method utilizing corn's by-products simultaneously to produce epsilon-polylysine and protein feed is provided, maize peel, the plumule dregs of rice, corn steep liquor, glucose mother liquid are integrated by the method, effectively can utilize the Carbon and nitrogen sources in accessory substance, the protein content of maize peel and the plumule dregs of rice can be promoted again, can large-scale production and application, provide an effective approach for enterprise solves corn's by-products problem.
Technical scheme of the present invention is: a kind of method utilizing corn's by-products simultaneously to produce epsilon-polylysine and protein feed, comprises the following steps:
(1) add water stirring respectively in maize peel and the plumule dregs of rice, makes its water content all be greater than 60%;
(2) in the maize peel and each material base of the plumule dregs of rice of step (1) gained, add amylase respectively and cellulase carries out enzymolysis, the mass ratio of amylase and cellulase is 1:1 ~ 1:3, and enzyme-added total amount is not less than 0.1% of each material basic weight amount; Hydrolysis temperature scope is 30 ~ 70 DEG C, and the processing time is at least 15 h;
(3) after enzymolysis terminates, respectively maize peel enzymolysis mother liquor and plumule dregs of rice enzymolysis mother liquor are carried out Separation of Solid and Liquid, obtain maize peel enzymolysis filtrate I, maize peel enzymolysis filter residue I, plumule dregs of rice enzymolysis filtrate I and plumule dregs of rice enzymolysis filter residue I respectively; Double the water washing maize peel enzymolysis filter residue I of maize peel enzymolysis filtrate I by quality, double the water washing plumule dregs of rice enzymolysis filter residue I of plumule dregs of rice enzymolysis filtrate I by quality; Again carry out Separation of Solid and Liquid and obtain maize peel enzymolysis filtrate II, maize peel enzymolysis filter residue II, plumule dregs of rice enzymolysis filtrate II and plumule dregs of rice enzymolysis filter residue II;
(4) each total filter residue of the middle gained maize peel of step (3) and the plumule dregs of rice, water content is 55 ~ 60%, after using drying plant drying respectively, obtains protein feed maize peel and the plumule dregs of rice;
(5) the whole filtrate of gained I in step (3) mixed with whole filtrate II, then mix with glucose mother liquid, this part syrup is as fermenting carbon source; Separately get corn steep liquor in corn's by-products as fermentation organic nitrogen source;
(6) fermentation medium components of epsilon-polylysine is: syrup, corn steep liquor, (NH
4)
2sO
4and MgSO
4;
(7) according to the epsilon-polylysine zymotechnique of routine, with the described culture medium fermentation production of epsilon-polylysine of step (6).
Syrup in described step (6) comprises filtrate I, filtrate II and glucose mother liquid.
In described step (7), the temperature of fermentation is 25 ~ 40 DEG C.
In described step (7), fermentation epsilon-polylysine producing strains used is streptomyces albus
s.albulus, streptomyces lydicus
s.lydicus, kitasatosporia
kitasatospora.sp, grey orange streptomycete
s.griseoaurantiacus, streptomyces diastatochromogenes
s.diastatochromogenes, bacillus subtilis
bacillus subtilis, streptomyces graminearus
s.graminearus, streptomyces hygroscopicus
s.hygroscopicus, grey brown streptomycete
s.griseofuscus, european bird cherry streptomycete
s.padanusin at least one.
Beneficial effect of the present invention is: the method for the invention amylase and cellulase process maize peel and the plumule dregs of rice, become reduced sugar by starch wherein with few fibers matter enzymolysis.Maize peel and the plumule dregs of rice carry out Separation of Solid and Liquid after enzymolysis, and filtrate produces epsilon-polylysine as carbon source through fermentation, as protein feed after filter residue oven dry.The protein content of ordinary maize skin is 7-10%, and conventional plumule cake protein content is 16-18%, and the cornbran protein content obtained in the present invention is 12-20%, and plumule cake protein content is 25-35%.The method of the invention has the following advantages:
1, production cost low, small investment, instant effect, high efficiency.
2, enzymolysis and fermentation condition extensive, the cycle is short, and production efficiency is high.
3, the maize peel in corn's by-products, the plumule dregs of rice, glucose mother liquid, corn steep liquor are effectively integrated, can fermentation production of epsilon-polylysine, improve again the added value of maize peel and the plumule dregs of rice.
The present invention relates to first with corn's by-products fermentation production of epsilon-polylysine and the added value promoting maize peel and the plumule dregs of rice, for corn deep processing industry process accessory substance provides an effective solution route, has great economic benefit and social benefit.
Detailed description of the invention
Be below embodiment, the present invention is further described, but do not limit the present invention.
Embodiment 1
(1) get maize peel 1 kg, add water 3 kg, then material moisture is 75%; Get the plumule dregs of rice 1 kg, add water 3 kg,
Then material moisture is 75%;
(2) in two kinds of materials, add amylase 1.5 g respectively, cellulase 1.5 g, namely enzyme concentration is 0.15% of material butt.
After stirring, material is put into the baking oven of 50 DEG C, place 24 h;
(3) taken out by the material of maize peel and the plumule dregs of rice, respectively with three layers of gauze parcel, firmly Separation of Solid and Liquid is carried out in extruding, obtains
Obtain filtrate and filter residue.Maize peel material can obtain filtrate 1.5 kg, filter residue 2.5 kg; Plumule dregs of rice material can obtain filtrate 1.8 kg, filter residue 2.2 kg;
(4) in maize peel filter residue, add 3 kg water, mix rear again with gauze extruding, acquisition filtrate 3.2 kg,
Filter residue 2.3 kg; In plumule dregs of rice filter residue, add 3 kg water, again with gauze extruding after mixing, obtain filtrate 3.2 kg, filter residue 2 kg;
(5) respectively the plumule dregs of rice filter residue of the maize peel filter residue of 2.3 kg and 2 kg is put into baking oven, after 80 DEG C of oven dry, obtain maize peel 0.7 kg, protein content is 14%; Obtain the plumule dregs of rice 0.55 kg, protein content is 30%.
Slant medium: glucose 1.0%, corn steep liquor 1.0%, agar 2.0%, pH 7.5,115 DEG C of sterilizing 20 min.
Seed culture medium: glucose 2.0%, corn steep liquor 1.0%, magnesium sulfate 0.1%, pH 7.5,115 DEG C of sterilizing 20 min.
In this embodiment, first time Separation of Solid and Liquid acquisition maize peel filtrate 1.5 kg, plumule dregs of rice filtrate 1.8 kg; Second time Separation of Solid and Liquid obtains maize peel filtrate 3.2 kg, plumule dregs of rice filtrate 3.2kg.These filtrates are mixed, totally 9.7 kg.
Fermentation medium: filtrate 10.3 kg, glucose mother liquid 1 kg, corn steep liquor 0.5 kg, (NH
4)
2sO
4100 g, MgSO
410 g, 115 DEG C of sterilizing 20 min.
By streptomyces albus
s. albulusspore access slant medium, 30 DEG C cultivate 7 days, obtain strain inclined plane; Then connect a ring slant strains and enter seed culture medium, cultivate 1 day for 30 DEG C, obtain the seed liquor of streptomyces albus.
Be load above-mentioned fermentation medium in the fermentation tank of 15 L at volume, by 1 L streptomyces albus seed liquor access fermentation medium.Fermentation temperature is 30 DEG C, and initial pH is 7.5, and throughput is 1.0 vvm, and speed of agitator is 500 rpm.When pH drops to 4.0 naturally, in tank, stream adds the ammoniacal liquor that concentration is 15%, and maintaining zymotic fluid pH is 4.0 ± 0.2, when concentration of reduced sugar is lower than fermentation ends during 5 g/L.Concentration now containing epsilon-polylysine in zymotic fluid is 15 g/L.
Embodiment 2
(1) get maize peel 1 kg, add water 3 kg, then material moisture is 75%; Get the plumule dregs of rice 1 kg, add water 3 kg, then material moisture is 75%.
(2) in two kinds of materials, add amylase 3 g respectively, cellulase 3 g, namely enzyme concentration is 0.3% of material butt.After stirring, material is put into the baking oven of 50 DEG C, place 24 h.
(3) maize peel and the plumule dregs of rice are taken out, respectively with three layers of gauze parcel, firmly extruding is tightened, and obtains filtrate and filter residue.Maize peel material can obtain filtrate 1.7 kg, filter residue 2.3 kg; Plumule dregs of rice material can obtain filtrate 2 kg, filter residue 2 kg.
(4) in maize peel filter residue, add 3 kg water, again with gauze extruding after mixing, obtain filter residue 2 kg, filtrate 3.3 kg; In plumule dregs of rice filter residue, add 3 kg water, again with gauze extruding after mixing, obtain filter residue 1.7 kg, filtrate 3.3 kg.
(5) respectively the plumule dregs of rice filter residue of the maize peel filter residue of 2 kg and 1.7 kg is put into baking oven, 80 DEG C of oven dry, obtain maize peel 0.55 kg, protein content is 18%; Obtain the plumule dregs of rice 0.45 kg, protein content is 35%.
Slant medium: glucose 1.0%, corn steep liquor 1.0%, agar 2.0%, pH 7.5,115 DEG C of sterilizing 20 min.
Seed culture medium: glucose 2.0%, corn steep liquor 1.0%, magnesium sulfate 0.1%, pH 7.5,115 DEG C of sterilizing 20 min.
In this embodiment, first time Separation of Solid and Liquid acquisition maize peel filtrate 1.7 kg, plumule dregs of rice filtrate 2 kg; Second time Separation of Solid and Liquid obtains maize peel filtrate 3.3 kg, plumule dregs of rice filtrate 3.3 kg.These filtrates are mixed, totally 10.3 kg.
Fermentation medium: filtrate 10.3 kg, glucose mother liquid 1 kg, corn steep liquor 0.5 kg, (NH
4)
2sO
4100 g, MgSO
410 g, 115 DEG C of sterilizing 20 min.
By streptomyces albus
s. albulusspore access slant medium, 30 DEG C cultivate 7 days, obtain strain inclined plane; Then connect a ring slant strains and enter seed culture medium, cultivate 1 day for 30 DEG C, obtain the seed liquor of streptomyces albus.
Be load above-mentioned fermentation medium in the fermentation tank of 15 L at volume, by 1 L streptomyces albus seed liquor access fermentation medium.Fermentation temperature is 30 DEG C, and initial pH is 7.5, and throughput is 1.0 vvm, and speed of agitator is 500 rpm.When pH drops to 4.0 naturally, in tank, stream adds the ammoniacal liquor that concentration is 15%, and maintaining zymotic fluid pH is 4.0 ± 0.2, when concentration of reduced sugar is lower than fermentation ends during 5 g/L.Concentration now containing epsilon-polylysine in zymotic fluid is 15 g/L.
Claims (4)
1. utilize corn's by-products to produce a method for epsilon-polylysine and protein feed simultaneously, comprise the following steps:
(1) add water stirring respectively in maize peel and the plumule dregs of rice, makes its water content all be greater than 60%;
(2) in the maize peel and each material base of the plumule dregs of rice of step (1) gained, add amylase respectively and cellulase carries out enzymolysis, the mass ratio of amylase and cellulase is 1:1 ~ 1:3, and enzyme-added total amount is not less than 0.1% of each material basic weight amount; Hydrolysis temperature scope is 30 ~ 70 DEG C, and the processing time is at least 15 h;
(3) after enzymolysis terminates, respectively maize peel enzymolysis mother liquor and plumule dregs of rice enzymolysis mother liquor are carried out Separation of Solid and Liquid, obtain maize peel enzymolysis filtrate I, maize peel enzymolysis filter residue I, plumule dregs of rice enzymolysis filtrate I and plumule dregs of rice enzymolysis filter residue I respectively; Double the water washing maize peel enzymolysis filter residue I of maize peel enzymolysis filtrate I by quality, double the water washing plumule dregs of rice enzymolysis filter residue I of plumule dregs of rice enzymolysis filtrate I by quality; Again carry out Separation of Solid and Liquid and obtain maize peel enzymolysis filtrate II, maize peel enzymolysis filter residue II, plumule dregs of rice enzymolysis filtrate II and plumule dregs of rice enzymolysis filter residue II;
(4) each total filter residue of the middle gained maize peel of step (3) and the plumule dregs of rice, water content is 55 ~ 60%, after using drying plant drying respectively, obtains protein feed maize peel and the plumule dregs of rice;
(5) the whole filtrate of gained I in step (3) mixed with whole filtrate II, then mix with glucose mother liquid, this part syrup is as fermenting carbon source; Separately get corn steep liquor in corn's by-products as fermentation organic nitrogen source;
(6) fermentation medium components of epsilon-polylysine is: syrup, corn steep liquor, (NH
4)
2sO
4and MgSO
4;
(7) according to the epsilon-polylysine zymotechnique of routine, with the described culture medium fermentation production of epsilon-polylysine of step (6).
2. utilize corn's by-products to produce the method for epsilon-polylysine and protein feed according to claim 1 simultaneously, it is characterized in that, the syrup in described step (6) comprises filtrate I, filtrate II and glucose mother liquid.
3. utilize corn's by-products to produce the method for epsilon-polylysine and protein feed according to claim 1 simultaneously, it is characterized in that, in described step (7), the temperature of fermentation is 25 ~ 40 DEG C.
4. utilize corn's by-products to produce the method for epsilon-polylysine and protein feed according to claim 1 simultaneously, it is characterized in that, in described step (7), fermentation epsilon-polylysine producing strains used is streptomyces albus
s.albulus, streptomyces lydicus
s.lydicus, kitasatosporia
kitasatospora.sp, grey orange streptomycete
s.griseoaurantiacus, streptomyces diastatochromogenes
s.diastatochromogenes, bacillus subtilis
bacillus subtilis, streptomyces graminearus
s.graminearus, streptomyces hygroscopicus
s.hygroscopicus, grey brown streptomycete
s.griseofuscus, european bird cherry streptomycete
s.padanusin at least one.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104642729A (en) * | 2015-02-09 | 2015-05-27 | 山东西王糖业有限公司 | Method for producing protein feed by using corn deep-processing by-products as raw material |
| CN110200131A (en) * | 2019-07-01 | 2019-09-06 | 博益德（北京）生物科技有限公司 | A kind of single enzymatic hydrolysis mixed fermentation prepares the method and application of functional fiber feed |
| CN110574824A (en) * | 2018-07-25 | 2019-12-17 | 广州博采生物科技有限公司 | Utilization method of vegetable oil cake meal |
| CN111534474A (en) * | 2020-03-30 | 2020-08-14 | 江南大学 | Recombinant bacillus subtilis and application thereof in production of polylysine |
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Denomination of invention: Simultaneous production method using corn by-product e- Method for producing polylysine and protein feed Effective date of registration: 20211221 Granted publication date: 20180629 Pledgee: China Construction Bank Corporation Zouping sub branch Pledgor: SHANDONG XIWANG SUGAR INDUSTRY Co.,Ltd. Registration number: Y2021980015836 |