CN104961574A

CN104961574A - Culture solution for dendrobium officinale

Info

Publication number: CN104961574A
Application number: CN201510375314.4A
Authority: CN
Inventors: 傅明尧
Original assignee: Individual
Current assignee: Individual
Priority date: 2015-06-30
Filing date: 2015-06-30
Publication date: 2015-10-07
Anticipated expiration: 2035-06-30
Also published as: CN104961574B

Abstract

The invention discloses culture solution for dendrobium officinale. The culture solution comprises the following components by weight per litter: 20-30g/L of ammonium nitrate, 1-2g/L of monopotassium phosphate, 20-30g/L of potassium nitrate, 6-10g/L of calcium nitrate, 3-8g/L of magnesium sulfate, 2-4g/L of manganese sulfate, 0.5-1g/L of ferric sulfate, 0.5-1g/L of sodium molybdate, 0.5-1g/L of vitamin B1, 0.2-0.5g/L of vitamin B6, 0.5-1g/L of vitamin B5, 1-2g/L of Na2-EDTA, 0.5-1g/L of naphthylacetic acid, 0.2-0.6g/L of ferulic acid, 10-15g/L of banana puree, 3-8g/L of grape seeds, 0.1-0.5g/L of water-soluble carbon nano tube, 0.1-0.9g/L of indolebutyric acid, 5-8g/L of white sugar and 1-3g/L of agar. The culture solution disclosed by the invention has the advantages that since the grape seed powder is added into the culture medium, not only can the yield and the quality of the dendrobium officinale be improved, but also the biotransformation rate of the dendrobium officinale can be increased; since the ferulic acid is added into the culture medium, the indolebutyric acid is protected from being decarboxylated, and plays a full role in induction; the culture medium has rich various nutrient substances and plant hormones needed by rooting growth of the dendrobium officinale, extremely-high rooting rate and good growth of roots and stems of the dendrobium officinale can be ensured and simultaneously the content of crude polysaccharide can be increased.

Description

A kind of Dendrobium officinale culture solution

Technical field

The present invention relates to a kind of cultivation of Herba Dendrobii, particularly a kind of Dendrobium officinale culture solution, belongs to field of agricultural cultivation.

Background technology

Herba Dendrobii is perennial herb, belong to epiphytic orchid class, popular name Tiepi Fengdou, because its old stem is outward in black, have another name called for Herba hedyotis costatae, the stem of noble dendrobium is conventional rare Chinese medicine, and applicating history is long, be listed in top grade in Shennong's Herbal, now confirmed that it has nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, relieving cough and moistening lung, the improving eyesight that wets one's whistle, improves immune function of human body, anti-curing oncoma and cardiovascular disorder and improving water flood, prevents and treats cataract, the effect such as to promote longevity.But because growing environment is special and distribution limitation, and excavate for a long time, natural resources is increasingly exhausted, is classified as the natural crude drugs of focused protection by country and encourages artificial commerial growing Herba Dendrobii.

Along with the development of modern biology, carry out vegetative propagation by tissue culture more and more extensive.Tissue culture propagation has that the time is short, breeding coefficient is high, the virus-free pollution of plant, a large amount of nursery and Duo Ji nursery can be carried out, not only economy but also simple, and keep the good character of parent, be thus widely used in the production fields such as the preserving seed of various plants, plant detoxification and agricultural, gardening and forestry.

Chinese patent 201210502454.X discloses a kind of Dendrobium officinale culture solution, containing following component: saltpetre, ammonium nitrate, magnesium sulfate, manganous sulfate, zinc sulfate, potassium primary phosphate, copper sulfate, potassiumiodide, cobalt chloride, boric acid, Sodium orthomolybdate, vitamin V B1, vitamin V B6, vitamin V B5, glycine, ferrous sulfate, disodium ethylene diamine tetraacetate, calcium chloride, naphthylacetic acid, banana puree, mashed potato, white sugar, inositol, agar powder.This nutrient solution is the raw stem of short root enough fast, improves polysaccharide content in Herba Dendrobii, aminoacids content, but its Herba Dendrobii transplanting survival rate does not significantly improve.

Summary of the invention

The object of the present invention is to provide a kind of Dendrobium officinale culture solution.

The technical solution realizing the object of the invention is: a kind of Dendrobium officinale culture solution, comprise ammonium nitrate 20-30g/L, potassium primary phosphate 1-2g/L, saltpetre 20-30g/L, nitrocalcite 6-10g/L, magnesium sulfate 3-8g/L, manganous sulfate 2-4g/L, ferric sulfate 0.5-1g/L, Sodium orthomolybdate 0.5-1g/L, VITMAIN B1 0.5-1g/L, vitamin B6 0.2-0.5g/L, vitamin B5 0.5-1g/L, Na2-EDTA 1-2g/L, naphthylacetic acid 0.5-1g/L, forulic acid 0.2-0.6g/L, banana puree 10-15g/L, Semen Vitis viniferae 3-8g/L, water-soluble carbon nanometer tube 0.1-0.5g/L, indolebutyric acid 0.1-0.9g/L, white sugar 5-8g/L, agar 1-3g/L.

The pH value range of described nutrient solution is between 5.5-6.0.

The present invention compared with prior art, its remarkable advantage:

1. add Semen Vitis viniferae powder in the medium, containing abundant multiple amino acids, VITAMIN and mineral substance in Semen Vitis viniferae, not only can improve the quantity and quality of Herba Dendrobii, the biological transformation ratio of Herba Dendrobii can also be improved.

2. with the addition of forulic acid in the medium, a kind of natural phenolic compound, forulic acid protection indolebutyric acid avoids decarboxylation, makes indolebutyric acid give full play to inducing action.

3. substratum be rich in Herba Dendrobii take root grow up needed for various nutritive substance and plant hormone, the good growth of the rooting rate that Herba Dendrobii is high and rhizome can be guaranteed, improve the Crude polysaccharides content of Herba Dendrobii simultaneously.

Embodiment

Embodiment 1

A kind of Dendrobium officinale culture solution, comprises ammonium nitrate 20g/L, potassium primary phosphate 2g/L, saltpetre 30g/L, nitrocalcite 8g/L, magnesium sulfate 5g/L, manganous sulfate 3g/L, ferric sulfate 0.5g/L, Sodium orthomolybdate 1g/L, vitaminB10 .8g/L, vitamin B6 0.2g/L, vitamin B5 0.5g/L, Na2-EDTA 2g/L, naphthylacetic acid 0.5g/L, forulic acid 0.2g/L, banana puree 10g/L, Semen Vitis viniferae 8g/L, water-soluble carbon nanometer tube 0.5g/L, indolebutyric acid 0.8g/L, white sugar 5g/L, agar 2g/L.

Embodiment 2

A kind of Dendrobium officinale culture solution, comprises ammonium nitrate 25g/L, potassium primary phosphate 1g/L, saltpetre 25g/L, nitrocalcite 10g/L, magnesium sulfate 3g/L, manganous sulfate 4g/L, ferric sulfate 0.5g/L, Sodium orthomolybdate 0.5g/L, vitaminB10 .5g/L, vitamin B6 0.5g/L, vitamin B5 1g/L, Na2-EDTA 1g/L, naphthylacetic acid 1g/L, forulic acid 0.5g/L, banana puree 15g/L, Semen Vitis viniferae 3g/L, water-soluble carbon nanometer tube 0.4g/L, indolebutyric acid 0.1g/L, white sugar 5g/L, agar 3g/L.

Embodiment 3

A kind of Dendrobium officinale culture solution, comprises ammonium nitrate 30g/L, potassium primary phosphate 1.5g/L, saltpetre 20g/L, nitrocalcite 6g/L, magnesium sulfate 8g/L, manganous sulfate 2g/L, ferric sulfate 1g/L, Sodium orthomolybdate 0.6g/L, vitaminB10 .6g/L, vitamin B6 0.4g/L, vitamin B5 0.6g/L, Na2-EDTA1.2g/L, naphthylacetic acid 0.8g/L, forulic acid 0.3g/L, banana puree 12g/L, Semen Vitis viniferae 5g/L, water-soluble carbon nanometer tube 0.1g/L, indolebutyric acid 0.9g/L, white sugar 8g/L, agar 1g/L.

Comparative example 1

A kind of Dendrobium officinale culture solution, comprises ammonium nitrate 28g/L, potassium primary phosphate 2g/L, saltpetre 30g/L, nitrocalcite 8g/L, magnesium sulfate 5g/L, manganous sulfate 3g/L, ferric sulfate 0.5g/L, Sodium orthomolybdate 1g/L, vitaminB10 .8g/L, vitamin B6 0.2g/L, vitamin B5 0.5g/L, Na2-EDTA 2g/L, naphthylacetic acid 0.5g/L, forulic acid 0.2g/L, banana puree 10g/L, water-soluble carbon nanometer tube 0.5g/L, indolebutyric acid 0.8g/L, white sugar 5g/L, agar 2g/L.

Comparative example 2

A kind of Dendrobium officinale culture solution, comprises ammonium nitrate 20g/L, potassium primary phosphate 2g/L, saltpetre 30g/L, nitrocalcite 8g/L, magnesium sulfate 5g/L, manganous sulfate 3g/L, ferric sulfate 0.5g/L, Sodium orthomolybdate 1g/L, vitaminB10 .8g/L, vitamin B6 0.2g/L, vitamin B5 0.5g/L, Na2-EDTA 2g/L, naphthylacetic acid 0.5g/L, banana puree 10g/L, Semen Vitis viniferae 8g/L, water-soluble carbon nanometer tube 0.5g/L, white sugar 5g/L, agar 3g/L.

Aseptically getting long has that the length 2 ~ 2.5cm's of 3 ~ 5 stem segments have the Herba Dendrobii stem section of axillalry bud as explant, the nutrient solution and the common MS nutrient solution that explant are placed in respectively embodiment 1-3 and comparative example 1-2 are cultivated, culture condition: temperature 25 DEG C, illumination condition 2000LX, light application time 12h/d, cultivates and compares after 25 days.Obtained experimental data is as shown in the table:

Claims

1. a Dendrobium officinale culture solution, it is characterized in that, comprise ammonium nitrate 20-30g/L, potassium primary phosphate 1-2g/L, saltpetre 20-30g/L, nitrocalcite 6-10g/L, magnesium sulfate 3-8g/L, manganous sulfate 2-4g/L, ferric sulfate 0.5-1g/L, Sodium orthomolybdate 0.5-1g/L, VITMAIN B1 0.5-1g/L, vitamin B6 0.2-0.5g/L, vitamin B5 0.5-1g/L, Na2-EDTA 1-2g/L, naphthylacetic acid 0.5-1g/L, forulic acid 0.2-0.6g/L, banana puree 10-15g/L, Semen Vitis viniferae 3-8g/L, water-soluble carbon nanometer tube 0.1-0.5g/L, indolebutyric acid 0.1-0.9g/L, white sugar 5-8g/L, agar 1-3g/L.

2. Dendrobium officinale culture solution as claimed in claim 1, it is characterized in that, the pH value range of described nutrient solution is between 5.5-6.0.