CN104936682B - 微滴界面 - Google Patents

微滴界面 Download PDF

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Publication number
CN104936682B
CN104936682B CN201380068550.0A CN201380068550A CN104936682B CN 104936682 B CN104936682 B CN 104936682B CN 201380068550 A CN201380068550 A CN 201380068550A CN 104936682 B CN104936682 B CN 104936682B
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polar medium
volume
polar
membrane
hydrophobic
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Chinese (zh)
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CN104936682A (zh
Inventor
安德鲁·约翰·赫伦
詹森·罗伯特·海德
克莱夫·加文·布朗
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0002—Organic membrane manufacture
    • B01D67/0023—Organic membrane manufacture by inducing porosity into non porous precursor membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/12—Composite membranes; Ultra-thin membranes
    • B01D69/122—Separate manufacturing of ultra-thin membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B01D71/261—Polyethylene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/26—Polyalkenes
    • B01D71/262—Polypropylene
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/70—Polymers having silicon in the main chain, with or without sulfur, nitrogen, oxygen or carbon only
    • B01D71/701—Polydimethylsiloxane
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/76—Macromolecular material not specifically provided for in a single one of groups B01D71/08 - B01D71/74
    • B01D71/80—Block polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/35—Use of magnetic or electrical fields
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/36—Hydrophilic membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/38—Hydrophobic membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/30—Polyalkenyl halides
    • B01D71/32—Polyalkenyl halides containing fluorine atoms
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/58—Other polymers having nitrogen in the main chain, with or without oxygen or carbon only
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/145555—Hetero-N

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Manufacturing & Machinery (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
CN201380068550.0A 2012-10-26 2013-10-23 微滴界面 Active CN104936682B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261718982P 2012-10-26 2012-10-26
US61/718,982 2012-10-26
PCT/GB2013/052767 WO2014064444A1 (en) 2012-10-26 2013-10-23 Droplet interfaces

Publications (2)

Publication Number Publication Date
CN104936682A CN104936682A (zh) 2015-09-23
CN104936682B true CN104936682B (zh) 2017-12-15

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Country Status (7)

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US (1) US9823235B2 (enExample)
EP (1) EP2911771B1 (enExample)
JP (1) JP6375301B2 (enExample)
CN (1) CN104936682B (enExample)
AU (1) AU2013336430B2 (enExample)
CA (1) CA2889664C (enExample)
WO (1) WO2014064444A1 (enExample)

Families Citing this family (140)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
KR102284661B1 (ko) * 2012-12-07 2021-08-02 옥스포드 유니버시티 이노베이션 리미티드 3d 프린팅에 의한 비말 어셈블리
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
EP3169800B1 (en) 2014-07-14 2020-11-18 Oxford University Innovation Limited Measurement of analytes with membrane channel molecules, and bilayer arrays
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN115851894B (zh) 2014-10-16 2025-06-27 牛津楠路珀尔科技股份有限公司 聚合物的分析
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
WO2017004504A1 (en) 2015-07-02 2017-01-05 The University Of Massachusetts Membrane and droplet-interface bilayer systems and methods
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
US20190093071A1 (en) 2016-03-01 2019-03-28 Oxford University Innovation Limited 3D Printing Of A Cellularised Scaffold
GB201603591D0 (en) 2016-03-01 2016-04-13 Isis Innovation Improved promotors and compositions
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
JP7364333B2 (ja) 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201615741D0 (en) 2016-09-15 2016-11-02 Univ College Cardiff Consultants Ltd Artificial cells
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
CN106474946A (zh) * 2016-10-17 2017-03-08 东华大学 一种具有亲水抗污性能的聚合物膜的制备方法
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
WO2018102218A1 (en) * 2016-11-29 2018-06-07 Kamterter Products, Llc Droplet for fuels
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
AU2018218531B2 (en) 2017-02-10 2021-01-28 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
US11293911B2 (en) 2017-12-28 2022-04-05 Korea Advanced Institute Of Science And Technology Multi-phase liquid composition, device for measuring permeability of drugs, and method for measuring permeability of drugs
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574197B (en) 2018-05-23 2022-01-05 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method and system.
CN112203767B (zh) 2018-05-24 2023-04-11 牛津纳米孔科技公司 电润湿装置中的液滴界面
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201811811D0 (en) 2018-07-19 2018-09-05 Oxford Nanopore Tech Ltd Method
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
JP2022502094A (ja) * 2018-10-08 2022-01-11 アドルフ・メークル・インスティチュート、ユニバーシティー・オブ・フリブール 細孔サイズ、膜親和性、安定性、および抗菌活性を高めるための、細孔形成ペプチドのオリゴヌクレオチドベースの調整
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
JP7499761B2 (ja) 2018-11-08 2024-06-14 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
WO2020109800A1 (en) 2018-11-28 2020-06-04 Oxford Nanopore Technologies Ltd. Sensing system and method of operation
CN113260449B (zh) * 2018-12-11 2023-09-29 豪夫迈·罗氏有限公司 用于膜中自限性蛋白质孔插入的系统和方法
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
KR20210138594A (ko) 2019-03-12 2021-11-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법
GB2580988B (en) 2019-03-19 2022-04-13 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
CA3134796A1 (en) 2019-04-09 2020-10-15 Oxford Nanopore Technologies Limited Pore
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
JP7730005B2 (ja) 2019-05-29 2025-08-27 ナンジン、ユニバーシティ 電極を使用しないナノポアによる分析物の検出
GB201915480D0 (en) 2019-10-25 2019-12-11 Oxford Nanopore Tech Ltd Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
CN118011004A (zh) 2019-12-02 2024-05-10 牛津纳米孔科技公开有限公司 使用纳米孔表征靶多肽的方法
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
GB202016874D0 (en) 2020-10-23 2020-12-09 Oxford Nanopore Tech Ltd Nanopore support structure and manufacture thereof
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
EP4168583A2 (en) 2020-06-18 2023-04-26 Oxford Nanopore Technologies plc Method of characterising a polynucleotide moving through a nanopore
CN115698331A (zh) 2020-06-18 2023-02-03 牛津纳米孔科技公开有限公司 使用检测器选择性地表征多核苷酸的方法
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
WO2022020461A1 (en) 2020-07-22 2022-01-27 Oxford Nanopore Technologies Inc. Solid state nanopore formation
WO2022051888A1 (zh) * 2020-09-08 2022-03-17 三诺生物传感股份有限公司 生物传感器的成膜组合物及其制备方法
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
CN117337333A (zh) 2021-05-19 2024-01-02 牛津纳米孔科技公开有限公司 用于补体链测序的方法
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
US20250006308A1 (en) 2021-11-29 2025-01-02 Oxford Nanopore Technologies Plc Nanopore measurement signal analysis
CN116297721A (zh) * 2021-12-21 2023-06-23 成都齐碳科技有限公司 成膜方法、包含膜的系统及应用
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
WO2023123434A1 (zh) * 2021-12-31 2023-07-06 深圳华大生命科学研究院 一种磷脂/聚合物仿生复合配方膜及其制备方法和应用
GB202202716D0 (en) 2022-02-28 2022-04-13 Oxford Nanopore Tech Plc Apparatus and methods for controlling insertion of a membrane channel into a membrane
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
CN115068336A (zh) * 2022-06-15 2022-09-20 厦门承葛生物科技有限公司 一种基于液滴微流控的肠道菌群包埋工艺
KR20250044691A (ko) 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250048551A (ko) 2022-08-09 2025-04-09 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어
CN119816360A (zh) * 2022-09-16 2025-04-11 深圳华大生命科学研究院 一种离子交联的仿生膜、其制备方法及应用
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
EP4608850A2 (en) 2022-10-28 2025-09-03 Oxford Nanopore Technologies PLC Pore monomers and pores
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
WO2024094966A1 (en) 2022-11-01 2024-05-10 Oxford Nanopore Technologies Plc Biochemical analysis system and method of controlling a biochemical analysis system
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
CN117384486B (zh) * 2023-02-10 2025-06-27 北京普译生物科技有限公司 一种混合仿生膜及其制备方法和应用
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
CN121057766A (zh) * 2023-05-04 2025-12-02 豪夫迈·罗氏有限公司 经修饰的三嵌段共聚物化合物及其使用方法
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
CN119327288A (zh) * 2023-07-18 2025-01-21 国家能源投资集团有限责任公司 一种用于海水电解制氢的正渗透膜及其制备方法
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407099D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407100D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032146A2 (en) * 1999-11-05 2001-05-10 Biocure, Inc. Amphiphilic polymeric vesicles
WO2001088025A1 (en) * 2000-05-16 2001-11-22 Biocure, Inc. Membranes formed from amphiphilic copolymers
CN1703199A (zh) * 2002-10-11 2005-11-30 伊迪亚股份公司 具有增强的可变形性、包含至少三种两亲性物质的聚集物，其用于改善通过半透屏障的转运以及非侵入性药物在体内、具体是通过皮肤的应用
WO2009024775A1 (en) * 2007-08-21 2009-02-26 Isis Innovation Limited Bilayers
CN102460150A (zh) * 2009-04-20 2012-05-16 新加坡科技研究局 囊泡系统及其用途

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807944A (en) * 1996-06-27 1998-09-15 Ciba Vision Corporation Amphiphilic, segmented copolymer of controlled morphology and ophthalmic devices including contact lenses made therefrom
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
JP5114702B2 (ja) * 2005-07-29 2013-01-09 国立大学法人 東京大学 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置
AT502713B1 (de) * 2005-10-19 2008-08-15 Univ Wien Bodenkultur Verfahren zur herstellung von lipid-membranen
GB0614835D0 (en) * 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules
WO2008120816A1 (ja) * 2007-03-30 2008-10-09 Tokyo Institute Of Technology 二分子膜の製造方法および二分子平面膜
US8101274B2 (en) * 2007-06-11 2012-01-24 Spedden Richard H Solid state membranes with surface-embedded glycosylated amphiphilic molecules and micelles formed therefrom
BRPI0706005A2 (pt) 2007-07-24 2009-03-17 Orbisat Da Amazonia Ind E Aero método e sistema de difusão de serviços digitais com recepção particularizada por região
US8293339B2 (en) * 2007-09-17 2012-10-23 Sri International, Inc. Droplet bilayers
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US9447152B2 (en) 2008-07-07 2016-09-20 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
GB0913823D0 (en) * 2009-08-07 2009-09-16 Isis Innovation Bilayers
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
GB201313121D0 (en) * 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032146A2 (en) * 1999-11-05 2001-05-10 Biocure, Inc. Amphiphilic polymeric vesicles
WO2001088025A1 (en) * 2000-05-16 2001-11-22 Biocure, Inc. Membranes formed from amphiphilic copolymers
CN1703199A (zh) * 2002-10-11 2005-11-30 伊迪亚股份公司 具有增强的可变形性、包含至少三种两亲性物质的聚集物，其用于改善通过半透屏障的转运以及非侵入性药物在体内、具体是通过皮肤的应用
WO2009024775A1 (en) * 2007-08-21 2009-02-26 Isis Innovation Limited Bilayers
CN102460150A (zh) * 2009-04-20 2012-05-16 新加坡科技研究局 囊泡系统及其用途

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