CN104885782B - A kind of asparagus breeding method of new high biological transformation ratio - Google Patents

A kind of asparagus breeding method of new high biological transformation ratio Download PDF

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Publication number
CN104885782B
CN104885782B CN201510297219.7A CN201510297219A CN104885782B CN 104885782 B CN104885782 B CN 104885782B CN 201510297219 A CN201510297219 A CN 201510297219A CN 104885782 B CN104885782 B CN 104885782B
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mushroom
bag
parts
breeding method
transformation ratio
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CN104885782A (en
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郭年东
林贵富
叶家华
黄振珊
黄振飞
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Jiangxi Ganzhou Xingwan Modern Agriculture Development Co Ltd
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Jiangxi Ganzhou Xingwan Modern Agriculture Development Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Mushroom Cultivation (AREA)

Abstract

The invention discloses a kind of asparagus breeding method of new high biological transformation ratio.Including bag, sterilizing, precooling, inoculation, cultural hypha, flower bud, open bag, regeneration mushroom culture, adopt the steps such as mushroom;The breeding method of the present invention is compared with waiting conventional cultivation method, under conditions of production bag and bacterium germination management method are all consistent, the characteristics of with obvious high yield, high-quality, product color is consistent, stem even thickness, length is basically identical, and the commodity of product is greatly improved, yield relatively waits conventional cultivation method to improve and about improves more than 30~45%, and commodity value improves about 35%.

Description

A kind of asparagus breeding method of new high biological transformation ratio
Technical field
The present invention relates to fungus growing technique field, specially a kind of acupuncture needle mushroom culture side of new high biological transformation ratio Method.
Background technology
Asparagus is a kind of nutritious, appetizing edible mushroom, protein, vitamin, carbohydrate, mineral matter Equal size enriches, wherein most of essential amino acid, the especially lysine of the human body contained and arginic content are very high, it is beneficial In the development of human body brain cell, therefore it is referred to as " increase intelligence mushroom ", it also has good medical value in addition, commonly using can be with Prevent liver system disease and gastrointestinal ulceration, reduction cholesterol and anti-cancer, effect of anticancer, be loved by people.
Current asparagus typically uses growing straight method cultural method, and it is not high that this cultural method has a fruiting amount, product color Inconsistent, stem thickness is uneven, the defect such as length is uneven, causes the commodity value of asparagus low.
The content of the invention
The technical problem to be solved in the present invention is to overcome existing defect there is provided a kind of acupuncture needle of new high biological transformation ratio Mushroom culture method.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme:
A kind of asparagus breeding method of new high biological transformation ratio, including following steps:
1), bag
After culture medium for golden mushroom pack, the 180min that sterilized by mushroom bag at 120 DEG C, under 0.125MPa is sterilized;After sterilizing The heat-insulated cleaning system for opening insulated room opens exhausting simultaneously, after 20-25 minutes, closes ultraviolet lamp, opens illuminating lamp, opens Stove door;Move mushroom bag to fore-cooling room precooling more than 5 hours, treat that temperature is reduced to 60 DEG C to move to strong cold house, purification is opened by strong cold house System, an air intake not air draft;
2), it is inoculated with
Inoculation is completed in the range of laminar flow hood purification, the humidity of strain is 16 DEG C, and humidity is below 70%;
3), cultural hypha
Temperature control in culturing room is at 18-22 DEG C, and humidity was less than 60%, in culture the 5th day, started ventilation;Culture the 15 days, storehouse temperature is reduced 1-2 DEG C;Start to shake bag when being put in storage the 29-30 days, the globule in bacterium bag is shaken to cause drop and gas is given birth to Mycelia departs from charge level, and water-in-bag pearl can not be shaken above tampon;Shake after bag and storehouse temperature to be down to 12-15 DEG C every 2 days;
4), flower bud
Between in each channel, bedstead half height and position set up 1 fan, open within 24 hours, keep CO2 concentration to keep In below 4000ppm, daily illumination 12-18 hours;
5) bag, is opened
Mushroom bud opens bag when growing to 2-3 centimeters;
6), regeneration mushroom culture
The bacterium bag after bag will be opened and move into mushroom producing room, mushroom producing room temperature control is at 5-8 DEG C, and relative humidity is treated in 75%-80% Increase humidity is to 85%-90% after the lodging of needle point mushroom, when mushroom flower bud length to be regenerated is to 3-5cm, carries out bagging, then ventilation daily 0.2-0.5h, illumination 6-10h;At the 3-10 days of bagging, when mushroom handle length reaches 3-5 centimetres, sack is improved 2-3 lis again Rice;
7) mushroom, is adopted
When mushroom body is long 16-18 centimetres, harvesting in time.
Further, step 1) described in culture medium be 20-35 part of bagasse, 5-10 parts of brewex's grains, sheep manure 10-15 Part, 20-40 parts of dry cow dung, 35-40 parts of corncob, 3-7 parts of cotton seed hulls, 10-15 parts of dregs of beans, 6-10 parts of wheat bran, rice bran 32-44 Part, 2-3 parts of corn flour, 2 parts of land plaster, 0.1-2 parts of lime, 0.1-1 parts of yeast extract.
Further, the step 1) in sterilizing method be:
1), vacuum is run:Vacuum is -0.05MPa;
2) heating operation:45min is incubated after being warming up to 85 DEG C, 45min is incubated after being warming up to 105 DEG C, then heats to 120 ℃;
3), sterilizing operation:Sterilize 180min under 120 DEG C, 0.125MPa;
4) boil in a covered pot over a slow fire and put operation:30min;
5) exhaust operation:Open manual exhaust valve 60min;
6) operation is terminated:Pressure 0MPa.
Further, the step 1) temperature of Zhong Qiang cold houses is set in 18-20 DEG C
Further, the step 2) in strain granular size no more than 1cm2;Inoculum concentration is strain particle mulch Face more than 2/3;
Further, the step 5) in open bag sack be higher than 1 centimeter of charge level.
The breeding method of the present invention is all consistent in production bag and bacterium germination management method compared with waiting conventional cultivation method Under conditions of, the characteristics of with obvious high yield, high-quality, product color is consistent, and stem even thickness, length is basically identical, production The commodity of product is greatly improved, and yield relatively waits conventional cultivation method to improve and about improves more than 30-45%, and commodity value is improved about 35%.
Embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
A kind of asparagus breeding method of new high biological transformation ratio, including following steps:
1), bag
After culture medium for golden mushroom pack, the 180min that sterilized by mushroom bag at 120 DEG C, under 0.125MPa is sterilized;After sterilizing The heat-insulated cleaning system for opening insulated room opens exhausting simultaneously, after 20-25 minutes, closes ultraviolet lamp, opens illuminating lamp, opens Stove door;Move mushroom bag to fore-cooling room precooling more than 5 hours, treat that temperature is reduced to 60 DEG C to move to strong cold house, the temperature of strong cold house is set 18 DEG C are scheduled on, cleaning system, an air intake not air draft are opened by strong cold house;
The culture medium is 20 parts of bagasse, 10 parts of brewex's grains, 15 parts of sheep manure grain, 20 parts of dry cow dung, 40 parts of corncob, cotton 3 parts of seed shell, 10 parts of dregs of beans, 10 parts of wheat bran, 32 parts of rice bran, 3 parts of corn flour, 2 parts of land plaster, 0.1 part of lime, 1 part of yeast extract.
2), it is inoculated with
Inoculation is completed in the range of laminar flow hood purification, the humidity of strain is 16 DEG C, and humidity is below 70%;
3), cultural hypha
Temperature control in culturing room is at 18 DEG C, and humidity was less than 60%, in culture the 5th day, started ventilation;Cultivate the 15th My god, storehouse temperature is reduced by 1 DEG C;Start to shake bag when being put in storage the 29th day, the globule in bacterium bag is shaken to cause drop and aerial hyphae is taken off From charge level, and water-in-bag pearl can not be shaken above tampon;Shake after bag and storehouse temperature to be down to 12 DEG C every 2 days;
4), flower bud
Between in each channel, bedstead half height and position set up 1 fan, open within 24 hours, keep CO2 concentration to keep In below 4000ppm, daily illumination 12 hours;
5) bag, is opened
Mushroom bud opens bag when growing to 2 centimeters;
6), regeneration mushroom culture
The bacterium bag after bag will be opened and move into mushroom producing room, mushroom producing room temperature control is at 5 DEG C, and relative humidity treats needle point mushroom 75% Increase humidity after lodging to 85%, when mushroom flower bud length to be regenerated is to 3cm, carry out bagging, then divulge information 0.2h, illumination 6h daily;When When mushroom handle length reaches 3 centimetres, sack is improved to 2 centimetres again;
7) mushroom, is adopted
Embodiment 2
A kind of asparagus breeding method of new high biological transformation ratio, including following steps:
1), bag
After culture medium for golden mushroom pack, the 180min that sterilized by mushroom bag at 120 DEG C, under 0.125MPa is sterilized;
The method of sterilizing is:
1), vacuum is run:Vacuum is -0.05MPa;
2) heating operation:45min is incubated after being warming up to 85 DEG C, 45min is incubated after being warming up to 105 DEG C, then heats to 120 ℃;
3), sterilizing operation:Sterilize 180min under 120 DEG C, 0.125MPa;
4) boil in a covered pot over a slow fire and put operation:30min;
5) exhaust operation:Open manual exhaust valve 60min;
6) operation is terminated:Pressure 0MPa.
The heat-insulated cleaning system that insulated room is opened after sterilizing opens exhausting simultaneously, after 20-25 minutes, closes ultraviolet lamp, Illuminating lamp is opened, stove door is opened;Move mushroom bag to fore-cooling room precooling more than 5 hours, treat that temperature is reduced to 60 DEG C to move to strong cold house, The temperature of strong cold house is set in 20 DEG C, and cleaning system, an air intake not air draft are opened by strong cold house;
The culture medium is 35 parts of bagasse, 5 parts of brewex's grains, 10 parts of sheep manure grain, 40 parts of dry cow dung, 35 parts of corncob, cotton 7 parts of seed shell, 15 parts of dregs of beans, 6 parts of wheat bran, 44 parts of rice bran, 2 parts of corn flour, 2 parts of land plaster, 2 parts of lime, 0.1 part of yeast extract.
2), it is inoculated with
Inoculation is completed in the range of laminar flow hood purification, the humidity of strain is 16 DEG C, and humidity is below 70%;Strain granular size No more than 1cm2;Inoculum concentration is that strain particle covers charge level more than 2/3;
3), cultural hypha
Temperature control in culturing room is at 22 DEG C, and humidity was less than 60%, in culture the 5th day, started ventilation;Cultivate the 15th My god, storehouse temperature is reduced by 2 DEG C;Start to shake bag when being put in storage the 30th day, the globule in bacterium bag is shaken to cause drop and aerial hyphae is taken off From charge level, and water-in-bag pearl can not be shaken above tampon;Reducing temperature twice has shaken after bag and storehouse temperature to be down into 15 DEG C every 2 days;
4), flower bud
Between in each channel, bedstead half height and position set up 1 fan, open within 24 hours, keep CO2 concentration to keep In below 4000ppm, daily illumination 18 hours;
5) bag, is opened
Mushroom bud opens bag when growing to 3 centimeters;The sack for opening bag is higher than 1 centimeter of charge level.
6), regeneration mushroom culture
The bacterium bag after bag will be opened and move into mushroom producing room, mushroom producing room temperature control is at 8 DEG C, and relative humidity treats needle point mushroom 80% Increase humidity after lodging to 90%, when mushroom flower bud length to be regenerated is to 5cm, carry out bagging, then divulge information 0.5h, illumination 10h daily; The 10th day of bagging, when mushroom handle length reaches 5 centimetres, sack is improved again 3 centimetres;
7) mushroom, is adopted
During 18 centimetres of mushroom body length, harvesting in time.
Finally it should be noted that:The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic. Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., should be included in the present invention's Within protection domain.

Claims (5)

1. a kind of asparagus breeding method of new high biological transformation ratio, it is characterised in that including following steps:
1), bag
After culture medium for golden mushroom pack, the 180min that sterilized by mushroom bag at 120 DEG C, under 0.125MPa is sterilized;The method of sterilizing For:1), vacuum is run:Vacuum is -0.05MPa;2) heating operation:45min is incubated after being warming up to 85 DEG C, 105 DEG C are warming up to After be incubated 45min, then heat to 120 DEG C;3), sterilizing operation:Sterilize 180min under 120 DEG C, 0.125MPa;4) boil in a covered pot over a slow fire and put fortune Row 30min;5) exhaust operation:Open manual exhaust valve 60min;6) operation is terminated:Pressure 0Mpa;
The heat-insulated cleaning system that insulated room is opened after sterilizing opens exhausting simultaneously, after 20-25 minutes, closes ultraviolet lamp, opens Illuminating lamp, opens stove door;Move mushroom bag to fore-cooling room precooling more than 5 hours, treat that temperature is reduced to 60 DEG C and moves to strong cold house, strong cold house Open cleaning system, an air intake not air draft;
2), it is inoculated with
Inoculation is completed in the range of laminar flow hood purification, the temperature of strain is 16 DEG C, and humidity is below 70%;
3), cultural hypha
Temperature control in culturing room is at 18-22 DEG C, and humidity was less than 60%, in culture the 5th day, started ventilation;Cultivate the 15th day, Storehouse temperature is reduced 1-2 DEG C;Start to shake bag when being put in storage the 29-30 days, the globule in bacterium bag is shaken to cause drop and makes aerial hyphae Depart from charge level, and water-in-bag pearl can not be shaken above tampon;Shake after bag and storehouse temperature to be down to 12-15 DEG C every 2 days;
4), flower bud
Between in each channel, bedstead half height and position set up 1 fan, 24 hours open, keep CO2Concentration is maintained at Below 4000ppm, daily illumination 12-18 hours;
5) bag, is opened
Mushroom bud opens bag when growing to 2-3 centimeters;
6), regeneration mushroom culture
The bacterium bag after bag will be opened and move into mushroom producing room, mushroom producing room temperature control is at 5-8 DEG C, and relative humidity treats needle point in 75%-80% Increase humidity is to 85%-90% after mushroom lodging, when mushroom flower bud length to be regenerated is to 3-5cm, carries out bagging, and then divulge information 0.2- daily 0.5h, illumination 6-10h;At the 3-10 days of bagging, when mushroom handle length reaches 3-5 centimetres, sack is improved 2-3 centimetres again;
7) mushroom, is adopted
When mushroom body is long 16-18 centimetres, harvesting in time.
2. a kind of asparagus breeding method of new high biological transformation ratio as claimed in claim 1, it is characterised in that the training Support base be 20-35 part of bagasse, 5-10 parts of brewex's grains, sheep manure 10-15 parts, 20-40 parts of dry cow dung, 40 parts of corncob 35-, 3-7 parts of cotton seed hulls, 10-15 parts of dregs of beans, 6-10 parts of wheat bran, 32-44 parts of rice bran, 2-3 parts of corn flour, 2 parts of land plaster, lime 0.1- 2 parts, 0.1-1 parts of yeast extract.
3. a kind of asparagus breeding method of new high biological transformation ratio as claimed in claim 1, it is characterised in that the step The temperature of rapid 1) Zhong Qiang cold houses is set in 18-20 DEG C.
4. a kind of asparagus breeding method of new high biological transformation ratio as claimed in claim 1, it is characterised in that the step It is rapid 2) in strain granular size no more than 1cm2;Inoculum concentration is that strain particle covers charge level more than 2/3.
5. a kind of asparagus breeding method of new high biological transformation ratio as claimed in claim 1, it is characterised in that the step It is rapid 5) in open bag sack be higher than 1 centimeter of charge level.
CN201510297219.7A 2015-06-03 2015-06-03 A kind of asparagus breeding method of new high biological transformation ratio Active CN104885782B (en)

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Publication number Priority date Publication date Assignee Title
CN105917967A (en) * 2016-05-30 2016-09-07 福建建宁日鑫菌业科技有限公司 Mycelium culture control method of needle mushroom
CN106069178A (en) * 2016-06-12 2016-11-09 吴军 The cultural method of Flammulina velutiper (Fr.) Sing
CN106007833A (en) * 2016-07-11 2016-10-12 赖卫华 Needle mushroom compost and preparation method thereof
CN108901586A (en) * 2017-04-12 2018-11-30 邵阳市云新高科农业开发有限公司 A kind of cultural method of needle mushroom

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CN104620850A (en) * 2013-11-08 2015-05-20 许超群 High-yield regeneration cultivation method for needle mushrooms
CN103828602B (en) * 2014-03-21 2017-06-09 重庆市宽哥农业发展有限公司 A kind of method for cultivating asparagus
CN104221705A (en) * 2014-08-29 2014-12-24 江苏省天健生物科技有限公司 Process flow of enoki mushroom cultivation technology
CN104396573A (en) * 2014-12-19 2015-03-11 苏州市经纬农产品有限公司 Flammulina velutipes cultivation method
CN104488561A (en) * 2015-01-04 2015-04-08 哈尔滨伟平科技开发有限公司 Needle mushroom cultivation method

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