CN104807691B - 肽的回收方法、肽的检测方法及试剂 - Google Patents
肽的回收方法、肽的检测方法及试剂 Download PDFInfo
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- CN104807691B CN104807691B CN201510033385.6A CN201510033385A CN104807691B CN 104807691 B CN104807691 B CN 104807691B CN 201510033385 A CN201510033385 A CN 201510033385A CN 104807691 B CN104807691 B CN 104807691B
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012723 sample buffer Substances 0.000 description 1
- 150000003355 serines Chemical class 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000012089 stop solution Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- NXSIJWJXMWBCBX-NWKQFZAZSA-N α-endorphin Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=CC=C1 NXSIJWJXMWBCBX-NWKQFZAZSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014-010597 | 2014-01-23 | ||
| JP2014010597A JP6161548B2 (ja) | 2014-01-23 | 2014-01-23 | ペプチドの回収方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104807691A CN104807691A (zh) | 2015-07-29 |
| CN104807691B true CN104807691B (zh) | 2019-03-01 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510033385.6A Expired - Fee Related CN104807691B (zh) | 2014-01-23 | 2015-01-23 | 肽的回收方法、肽的检测方法及试剂 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US9840535B2 (https=) |
| EP (1) | EP2902407B1 (https=) |
| JP (1) | JP6161548B2 (https=) |
| CN (1) | CN104807691B (https=) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102015214414B4 (de) * | 2015-07-29 | 2020-10-22 | Berthold Technologies Gmbh & Co. Kg | Verfahren und System zur Ermittlung biologischer Eigenschaften von Proben |
| JP6675167B2 (ja) | 2015-08-28 | 2020-04-01 | シスメックス株式会社 | ペプチドの遊離方法及び回収方法、並びにペプチド遊離剤及び試薬キット |
| JP6979797B2 (ja) | 2017-05-31 | 2021-12-15 | シスメックス株式会社 | 固液分離方法、固液分離装置およびそれに用いるピペットチップ、粒子およびキット |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62266071A (ja) * | 1986-05-14 | 1987-11-18 | テルモ株式会社 | 血漿蛋白質分離装置 |
| JPH04309361A (ja) * | 1991-04-05 | 1992-10-30 | Terumo Corp | 血漿蛋白質分画分離剤および血漿蛋白質分画分離用器具 |
| CA2312048A1 (en) * | 1997-12-03 | 1999-06-10 | Zymogenetics, Inc. | Human thyroid protein zsig45 and dna encoding same |
| WO2005052593A1 (en) * | 2003-10-29 | 2005-06-09 | The University Of Leicester | Detection |
| EP2182003B1 (en) * | 2007-08-21 | 2014-01-22 | Mmt Co., Ltd | Peptide capable of binding to immunoglobulin |
| JP5689074B2 (ja) * | 2009-11-20 | 2015-03-25 | シスメックス株式会社 | 分析対象ペプチドの保護方法および回収方法 |
| JP5636331B2 (ja) * | 2011-04-28 | 2014-12-03 | シスメックス株式会社 | ペプチドの遊離方法および回収方法 |
-
2014
- 2014-01-23 JP JP2014010597A patent/JP6161548B2/ja not_active Expired - Fee Related
-
2015
- 2015-01-14 US US14/596,334 patent/US9840535B2/en not_active Expired - Fee Related
- 2015-01-23 CN CN201510033385.6A patent/CN104807691B/zh not_active Expired - Fee Related
- 2015-01-23 EP EP15152228.1A patent/EP2902407B1/en not_active Not-in-force
Also Published As
| Publication number | Publication date |
|---|---|
| CN104807691A (zh) | 2015-07-29 |
| EP2902407A1 (en) | 2015-08-05 |
| US9840535B2 (en) | 2017-12-12 |
| JP6161548B2 (ja) | 2017-07-12 |
| EP2902407B1 (en) | 2016-04-13 |
| US20150203533A1 (en) | 2015-07-23 |
| JP2015137978A (ja) | 2015-07-30 |
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