CN104782933A - Preparation method of environment-friendly bait for pinna - Google Patents
Preparation method of environment-friendly bait for pinna Download PDFInfo
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- CN104782933A CN104782933A CN201510127969.XA CN201510127969A CN104782933A CN 104782933 A CN104782933 A CN 104782933A CN 201510127969 A CN201510127969 A CN 201510127969A CN 104782933 A CN104782933 A CN 104782933A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses a preparation method of environment-friendly bait for pinna and belongs to the field of aquaculture. The method is characterized in that high quality benthic diatom is cultured by an optimized culture medium, magnetized water, green light, palm bark, a glass goldfish bowl and the like, and then the mixed bait for the pinna is prepared by taking the benthic diatom as a major component and adding a small amount of sheep manure, rabbit manure, porcine blood powder, yeast powder, corn distiller, potamogeton malaianus powder, water caltrop powder, hydrilla varticillata powder, bone tankage, earthworm powder, fly maggot powder, artemia powder, kelp powder, garlic powder, methionine, lysine, potato powder, locust powder, lucerne meal, clam worm powder, trash fish and spiral shell meat meal. The bait is scientific and reasonable in formulation, nutrient-rich and lower in cost, is used for feeding the pinna and does not pollute water, the pinna grows quickly, has a strong constitution and is high in survival rate, and the yield is increased by 160%.
Description
Technical field the invention belongs to aquaculture field, particularly relates to a kind of preparation method of pinniform pen shell environmental protection bait.
Background technology
Pinniform pen shell is a kind of sea mollusk, red also known as Jiang Yao, beautiful Yao, sea, is subordinate to Mollusca, lamellibranchiata, pen shell section.Mainly be distributed in Singapore, Malaysia, Korea S, China's Mainland, TaiWan, China are coastal, often perch in the sand mud substrate in the shallow sea dark to 20 meters, intertidal zone.Shell is large and thin, wide after paracone, wedge shape.Surface tool radial rib, rib has triangle slightly oblique rear clavula.Color light brown is to dark brown, slightly transparent during children.Byssus hair-like is flourishing.Uprightly silt is inserted with beak end, with byssus set seabed, when assembling in groups, seabed stone forest seemingly.Once settling down, no longer move throughout one's life, with unicellular alga and organic debris for staple food.Posterior adductor muscle is flourishing, and account for body long more than 1/3, edible, justify greatly, meat is tender delicious, nutritious, and its dried product is exactly the dried adductor of a pen shell.Pinniform pen shell mainly distributes the torrid zone and Subtropical Coastal, and China is the highest with Guangdong, ALONG COASTAL FUJIAN output.Japan, Mexico, Bolivia and Indian Ocean coastal state are used as ticbit.Coastal have common saying: sea cucumber abalone pen shell shellfish, and meaning and pen shell Becquerel and sea cucumber abalone compare favourably.Pen shell lives in abyssal floor, fishes for difficulty, and diver must dive seabed, can catch, therefore landings is less, and supply falls short of demand in outlet, and common fish market is difficult to see.In international market, pen shell shellfish is also fix a price by head size.Pen shell shellfish is except being rich in animal protein, calcium phosphate and vitamin, and Taurine content is also very high.The position that pen shell is the niciest is Bei Zhu, milky, and meat is tender and crisp, can directly dip in sauce and eat something rare, also can rinse food, steams, quick-fried, stir-fry etc. or make congee soup, is famous and precious delicacy, also can bag boiled dumpling, has a distinctive flavour.Shellfish juice or a kind of senior flavouring.Pen shell shell and cedductor all can be used as medicine.Shell is containing angle sclerotin, calcium carbonate etc.Pen shell shellfish is also rich in mucinoid, and the sugar by nearly 8% and 10% albumen form, and sugar is made up of N-acetyl glucose amine and galactosamine, galactolipin, fucose etc.Also containing myosin, actin, fibrin, paramyosinogen, tropomyosin bletilla ten several amino acids.Still containing adenosine triphosphate, phosphate, oxyindole oxidizing ferment.Visceral mass argentiferous, manganese etc.Shell has clearing heat and detoxicating, to relieve dizziness, high fever, infantile convulsions, epilepsy, etc. calmness merit, for preventing and treating hypertension, wet sore etc.Merit in the enriching yin and nourishing kidney of shellfish post tool, tune.According to " new compilation of materia medica ": under pen shell in controlled atmosphere, sharp the five internal organs, treatment is quenched one's thirst, place food in the abdomen that disappears." book on Chinese herbal medicine asks former " is said: pen shell can grow Kidney-Yin, only urine.For the disease such as the consume of prolonged illness essence and blood, the five internal organs virtual loss, weakness of the spleen and the stomach, malnutritive, diabetes, impotence and seminal emission, difficulty in urination and defecation.
Marine benthic diatom is the most important bait of pinniform pen shell.There is provided superior in quality, bait that quantity is sufficient ensures that pen shell grows up healthy and sound, survival rate is high, the important step of stable high yield.Past is normal causes that pinniform pen shell physique is thin and weak, survival rate is low, even mortality because of bait deficiency, although past a lot of scholar and researcher transports with regard to pinniform pen shell parent shellfish, accelerate the ripening, seedling fostering, semi, grow, adapt to sea area etc. and carried out large quantity research and test and had made great progress, but still to a certain degree with in scope having that bait is under-supply or of low quality, seed physique is poor, pinniform pen shell yields poorly, produce the problems such as shakiness.About Seedling production and the benthic diatom cultural method of pen shell, past Zhang Jian etc. discloses a kind of method for culturing seedlings (application number CN201010614627) of Pinna pectinata, this goal of the invention is to provide a kind of method for culturing seedlings of Pinna pectinata, comprise the following steps: select Pinna pectinata as close shellfish, cultivate through accelerating, close shellfish is ripe gradually.After being dried in the shade by close shellfish, with 30W ultraviolet lamps irradiates 3 hours, make ripe close shellfish discharge essence, ovum, essence, ovum are fertilized voluntarily in water.Be fertilized and develop into D shape veliger after 25 hours.When larvae development becomes D shape veliger, adopt 300 order trawlnets the collection of D shape veliger to be placed in nursery pond and cultivate.When foot appears in larva, throw in collector bag and collect seedling.Topple after being scrubbed by juvenile mollusk collector bag, spread by juvenile mollusk and be covered with in another pond described in sand; Food organisms after juvenile mollusk attachment are flat algae and Pyramimonas sp.This technology weed survival rate is high, but floor space is large, and troublesome poeration, easily pollutes, and the culture-cycle is long.In addition, Chen Yuezhong has invented a kind of method (application number CN201110094895) that the Bao Youti high-quality bait such as avette algae are cultivated in extensive slewing, specifically comprise: (1) collects the Bao larvae attachment substratum having the growth of a large amount of avette algae, cleaning film until adherance film surface totally but the golden yellow vitrina that still visible one deck is light in the sun; (2) film after cleaning is wrapped weight to be bundled into bouquet shape and to throw in filtering sea, apply benthic diatom slewing and cultivate nutrient solution: NaNO
350-80mg/L, NaH
2pO
4h
2o 0.3-1mg/L, FeC
6h
5o
75H
2o 1-2mg/L, Na
2siO
39H
2o 3-5mg/L, diatom nutrient 2-4mg/L, under 1500-2500lux illumination, 5-10 days is cultivated in continuous charge, film is taken out and again cleans, (3) continue to cultivate in the fresh filtering sea cultivating nutrient solution containing benthic diatom slewing, after 4-7 days, take out and clean, cultivate continuation in the fresh filtering sea of nutrient solution in cultivation containing benthic diatom slewing more afterwards and cultivate.The cultivation time of Bao Youti high-quality bait benthic diatom can be foreshortened to 15-30 days by 20-100 days by this invention.But this technical matters is complicated, and cost is higher, be difficult to promote.
In order to overcome the deficiency of current technology, the invention provides a kind of preparation method of pinniform pen shell environmental protection bait, technological core is first with the culture medium optimized, magnetized drinking water, purple light, fortune windmillpalm sheath-fibre, glass goldfish tanks etc. cultivate high-quality benthic diatom, then be key component with benthic diatom, add a small amount of sheep excrement, rabbit excrement, pig blood, dusty yeast, maize alcohol stillage, p.malaianus, water caltrop, hydrilla verticillata, animal tankage, earthworm powder, fly maggot powder, halogen worm, Kelp Powder, garlic powder, methionine, lysine, dehydrated potato powder, locust powder, clover, clam worm powder, trash fish, snail shell digested tankage makes pinniform pen shell mixed feed.Compared with conventional art, the technology of the present invention novelty shows: 1) this bait formula science, rationally, nutritious, cost is lower, in order to pinniform pen shell of throwing something and feeding, growth is fast, and physique is healthy and strong, and survival rate is high, and output can improve 160%; 2) attachment of benthic diatom adopts mesh sheet, poplar root, film, pasture and water etc. in the past, and cost is high, easily pollutes, operation inconvenience, I adopts fortune windmillpalm sheath-fibre now, and advantage is that fortune windmillpalm sheath-fibre fibrous root is many, can be large for the surface area of benthic diatom attachment, resistance to rotten, resistance to immersion, pollute little, cost is low; 3) in cultivation benthic diatom process, adopt optimization culture liquid, fertilizer and inorganic fertilizer, quick-acting fertilizer and slow-release fertilizer collocation mixing are used, and growth of diatom algae is fast, and output is high; 4) adopt magnetized drinking water and green light to cultivate benthic diatom, growth is fast, and output is high, and in cell, high unsaturated fatty acid EPA, DHA content obviously increase, and is conducive to pinniform pen shell and grows; 5) adopt fishbowl air-charging incubation diatom, scrub conveniently, output is high, and light transmission is good, and cost is low, not easily breaks up or scratches, reusable, not easily pollutes; 6) after cultivation terminates, the collection of diatom in the past more adopts centrifugal process, power consumption is large, centrifuge is expensive, cost is too high, and this technology adopts poly-phosphorus iron chloride (PPFC), poly-phosphorus aluminium chloride (PPAC) and ferric-polysilicate (PSF) to make compound precipitants, spends few, precipitation is completely, thoroughly, rapidly, simple to operation; 7) benthic diatom growth rhythm is respected, the early, middle and late phase of cultivating provides different light time, intensity and temperature, light application time is shorter in early days, intensity is less, avoid high light to cause light to injure to frustule, comparatively intense light irradiation and higher temperature are provided late period, be conducive to the high unsaturated fatty acid such as EPA, DHA in cell and accumulate in a large number, and at cultivation middle and later periods extra-nutrition salt, facilitate diatom and continue, grow fast.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides a kind of preparation method of pinniform pen shell environmental protection bait, step is as follows:
The preparation of step 1 benthic diatom nutrient solution
Nutrient solution formula (by weight): containing the industrial ammonia 1.2 parts of ammonia 25-28%, cow dung leachate 1 part, human urine 0.4 part, peptide urea 0.1 part, 0.03 part, ammonium sulfate, 0.2 part, ammonium nitrate, potassium sulfate 0.2 part, 0.5 part, potassium chloride, IBDU 0.1 part, oxamides 0.2 part, ammonium magnesium phosphate 0.05 part, APP 0.08 part, 0.02 part, calcium polyphosphate potassium, sodium metasilicate 0.6 part, herbal liquid medicine 1.2 parts, polyamines 0.001 part, abscisin 0.004 part, sodium acid carbonate 3 parts, soil extract 1.2 parts, ooze extract 1 part, Sodium Benzoate 0.02 part, potassium sorbate 0.002 part, plant ash 0.4 part, scalding and magnetization after 1000 parts, clean seawater.Abundant mixing, stir after, the acetic acid solution (volume ratio) with 15% and sodium acid carbonate (weight ratio) adjust ph of 20% are to 7.5-7.8.
The collection of step 2 benthic diatom
Get fortune windmillpalm sheath-fibre, be bundled into bundle, running water is cleaned, be placed in the Porcelain Jar of the liquor potassic permanganate filling 80ppm and soak 8 hours, rinse with running water, hang over that coastal waters (bank 300-5000 rice off sea) sea-tangle is floating to be cut down, in underwater, 0.5-2 rice is dark, hang 7-10 days, namely fortune windmillpalm sheath-fibre attached to a large amount of benthic diatom, as the algae kind that next step is cultivated, be brought up, put into and fill in the Plastic Drum of ordinary sea water, for subsequent use;
The cultivation of step 3 benthic diatom
Get a simple glass goldfish jar, cuboid, volume 200 liters, wash, with the liquor potassic permanganate soaking disinfection 5 hours of 80ppm, outwell, with distilled water flushing 3 times, add nutrient solution described in step 1 120 liters, the fortune windmillpalm sheath-fibre 3-4 being attached with benthic diatom put into described in step 2 restraints, and goldfish jar mouth is put a batten, with cord, fortune windmillpalm sheath-fibre be suspended in fish jar instead of sink at the bottom of fish jar, air-charging incubation, aeration quantity should not be too large, allows frustule float and not sink.Intensity of illumination 600-1200lux, illumination should not be too strong, otherwise green algae is understood amount reproduction thus suppresses the growth of diatom.Fish jar covering suitable for reading one block of glass, with the bacterium in blocks dust and air, virus, assorted algae etc., the gas be filled with in nutrient solution can distribute from batten.Glass jar and glass above, coated with green plastic film, are irradiated into the spectrum mainly green light of algae liquid like this, are conducive to synthesis and the accumulation of the high unsaturated fatty acids such as algae EPA, DHA.Glass jar is placed in microdisk electrode room and cultivates, air-conditioning temperature control, and fluorescent tube irradiates, first 5 days of cultivation, control temperature 18 DEG C, intensity of illumination 600-900lux, light dark period L: D=16h: 8h, namely every day 16 h light, 8 h dark.Cultivate 6-9 days, adjust the temperature to 21 DEG C, intensity of illumination 900-1200lux, light dark period L: D=20h: 4h, namely every day 20 h light, 4 h dark.Be cultured to the 6th day, add the nutrient solution 10 liters described in step 1, to supplement used up nutritive salt, be cultured to the 7th day, add distilled water 5 liters, to dilute algae liquid and to supplement the moisture vapored away; Be cultured to the 9th day, diatom density can reach 1,200 ten thousand milliliters/milliliter, stops cultivating, and can collect frustule.
The collection of step 4 benthic diatom
Get poly-phosphorus iron chloride (PPFC), poly-phosphorus aluminium chloride (PPAC) and ferric-polysilicate (PSF) (commercially available), in poly-phosphorus iron chloride: poly-phosphorus aluminium chloride: ferric-polysilicate=1: the ratio (weight ratio) of 2: 0.6 fully mixes, make compound precipitants, the amount adding 120 milligrams by often liter of algae liquid adds compound precipitants, stir, leave standstill after 36 hours, most frustule sinks to bottom, abandon supernatant, bottom yellow green toothpaste-like diatom algae cream can be obtained, collect with hard spoon, load beaker, preservative film seals, be placed in-4 DEG C of refrigerators, save backup.
The making of step 5 environmental protection mixed feed
Benthic diatom algae cream 35 parts, 1.5 parts, sheep excrement, 0.5 part, rabbit excrement, Swine blood meal 0.2 part, dusty yeast 0.4 part, maize alcohol stillage 2.4 parts, p.malaianus 0.3 part, water caltrop 0.7 part, hydrilla verticillata 0.5 part, animal tankage 0.1 part, earthworm powder 0.8 part, fly maggot powder 0.5 part, halogen worm 0.3 part, Kelp Powder 1.2 parts, 0.2 part, garlic powder, methionine 1.4 parts, lysine 0.7 part, dehydrated potato powder 5 parts, 1 part, locust powder, alfalfa meal 3 parts, 0.8 part, clam worm powder, trash fish 1.4 parts, snail shell digested tankage 2.4 parts.Often kind of raw material dries or 65 DEG C of drying machine dryings, and break into powdery, particle diameter is less than 40 microns, and mixing and blending machine fully stirs, mix, and environmental protection bait completes.
Step 6 mixed feed feeding method
When throwing something and feeding, get a Plastic Drum, add mixed feed, what add 25 times (weight ratios) again boils cooled seawater, be stirred well to evenly, make scattered paste shape, evenly can splash towards the Zhong Quan pond, pond of cultivating pen shell, early, middle and late each 1 time of every day, each feeding volume accounts for 0.2% of pen shell gross weight by bait weight.
Beneficial outcomes
In order to overcome the deficiency of current technology, the invention provides a kind of preparation method of pinniform pen shell environmental protection bait, technological core first cultivates high-quality benthic diatom with the culture medium, magnetized drinking water, purple light, fortune windmillpalm sheath-fibre, glass goldfish tank etc. optimized, after be key component with benthic diatom, add a small amount of sheep excrement, rabbit excrement, pig blood, dusty yeast, maize alcohol stillage, p.malaianus powder, water caltrop powder, hydrilla verticillata powder, fish meal, animal tankage, earthworm powder, fly maggot powder, artemia powder, Kelp Powder etc. make pinniform pen shell mixed feed.Compared with conventional art, novelty of the present invention shows: 1) this bait formula science, rationally, nutritious, cost is lower, in order to pinniform pen shell of throwing something and feeding, growth is fast, and physique is healthy and strong, and survival rate is high, and output is high; 2) attachment of benthic diatom adopts waste and old mesh sheet, poplar root, film, pasture and water etc. in the past, and cost is high, easily pollutes, operation inconvenience, existing I adopts fortune windmillpalm sheath-fibre, and advantage is that fortune windmillpalm sheath-fibre fibrous root is many, can be large for the surface area of benthic diatom attachment, resistance to rotten, resistance to immersion, pollute little, cost is low; 3) in cultivation benthic diatom process, adopt optimization culture liquid, fertilizer and inorganic fertilizer, quick-acting fertilizer and slow-release fertilizer collocation mixing are used, and growth of diatom algae is fast, and output is high; 4) adopt magnetized drinking water and green light to cultivate benthic diatom, growth is fast, and output is high, and in cell, high unsaturated fatty acid EPA, DHA content obviously increase, and is conducive to pinniform pen shell and grows; 5) adopt fishbowl air-charging incubation diatom, scrub conveniently, output is high, and light transmission is good, and cost is low, not easily breaks up or scratches, reusable, not easily pollutes; 6) after cultivation terminates, the collection of diatom in the past more adopts centrifugal process, power consumption is large, centrifuge is expensive, cost is too high, and this technology adopts poly-phosphorus iron chloride (PPFC), poly-phosphorus aluminium chloride (PPAC) and ferric-polysilicate (PSF) to make compound precipitants, spends few, precipitation is completely, thoroughly, rapidly, simple to operation; 7) benthic diatom growth rhythm is respected, the early, middle and late phase of cultivating provides different illumination intensity and temperature, light application time is shorter in early days, intensity is less, avoid high light to cause light to injure to frustule, comparatively intense light irradiation and higher temperature are provided late period, be conducive to the high unsaturated fatty acid such as EPA, DHA in cell and accumulate in a large number, and at cultivation middle and later periods extra-nutrition salt, facilitate diatom and continue, grow fast.
Detailed description of the invention
A preparation method for pinniform pen shell environmental protection bait, step is as follows:
The preparation of step 1 benthic diatom nutrient solution
Nutrient solution formula (by weight): containing the industrial ammonia 1.2 parts of ammonia 25%-28%, cow dung leachate 1 part, people urinates liquid 0.4 part, peptide urea 0.1 part, 0.03 part, ammonium sulfate, 0.2 part, ammonium nitrate, potassium sulfate 0.2 part, 0.5 part, potassium chloride, IBDU 0.1 part, oxamides 0.2 part, ammonium magnesium phosphate 0.05 part, APP 0.08 part, 0.02 part, calcium polyphosphate potassium, sodium metasilicate 0.6 part, herbal liquid medicine 1.2 parts, polyamines 0.001 part, abscisin 0.004 part, sodium acid carbonate 3 parts, soil extract 1.2 parts, ooze extract 1 part, Sodium Benzoate 0.02 part, potassium sorbate 0.002 part, plant ash 0.4 part, scalding and magnetization after 1000 parts, clean seawater, abundant mixing, stir after, the acetic acid solution (volume ratio) with 15% and sodium acid carbonate (weight ratio) adjust ph of 20% are to 7.5-7.8,
The collection of step 2 benthic diatom
Get fortune windmillpalm sheath-fibre, be bundled into bundle, running water is cleaned, be placed in the Porcelain Jar of the liquor potassic permanganate filling 80ppm and soak 8 hours, rinse with running water, hang over that coastal waters (bank 300-5000 rice off sea) sea-tangle is floating to be cut down, in underwater, 0.5-2 rice is dark, hang 7-10 days, namely fortune windmillpalm sheath-fibre attached to a large amount of benthic diatom, as the algae kind that next step is cultivated, be brought up, put into and fill in the Plastic Drum of ordinary sea water, for subsequent use;
The cultivation of step 3 benthic diatom
Get a simple glass goldfish jar, cuboid, volume 200 liters, wash, with the liquor potassic permanganate soaking disinfection 5 hours of 80ppm, outwell, with distilled water flushing 3 times, add nutrient solution described in step 1 120 liters, the fortune windmillpalm sheath-fibre 3-4 being attached with benthic diatom put into described in step 2 restraints, and goldfish jar mouth is put a batten, with cord, fortune windmillpalm sheath-fibre be suspended in fish jar instead of sink at the bottom of fish jar, air-charging incubation, aeration quantity should not be too large, allows frustule float and not sink.Intensity of illumination 600-1200lux, illumination should not be too strong, otherwise green algae is understood amount reproduction thus suppresses the growth of diatom; Fish jar covering suitable for reading one block of glass, the gas overflowed in nutrient solution can distribute from batten; Glass jar and glass above, coated with the plastic sheeting of green, irradiate the spectrum mainly green light into algae liquid like this, are conducive to synthesis and the accumulation of the high unsaturated fatty acids such as algae EPA, DHA; Glass jar is placed in microdisk electrode room and cultivates, air-conditioning temperature control, and fluorescent tube irradiates, first 5 days of cultivation, control temperature 18 DEG C, intensity of illumination 600-900lux, light dark period L: D=16h: 8h, namely every day 16 h light, 8 h dark; Cultivate 6-9 days, adjust the temperature to 21 DEG C, intensity of illumination 900-1200lux, light dark period L: D=20h: 4h, namely every day 20 h light, 4 h dark.Be cultured to the 6th day, add the nutrient solution 10 liters described in step 1, with the consumption of the salt that supplements the nutrients, be cultured to the 7th day, add distilled water 5 liters, to dilute algae liquid and to supplement the moisture vapored away; Be cultured to the 9th day, diatom density can reach 1,200 ten thousand milliliters, stops cultivating, and can collect frustule;
The collection of step 4 benthic diatom
Get poly-phosphorus iron chloride (PPFC), poly-phosphorus aluminium chloride (PPAC) and ferric-polysilicate (PSF) (commercially available), in poly-phosphorus iron chloride: poly-phosphorus aluminium chloride: ferric-polysilicate=1: the ratio (weight ratio) of 2: 0.6 fully mixes, make compound precipitants, the amount adding 120 milligrams by often liter of algae liquid adds compound precipitants, stir, leave standstill after 36 hours, most frustule sinks to bottom, abandon supernatant, bottom yellow green toothpaste-like diatom algae cream can be obtained, collect, load beaker, preservative film seals, be placed in-4 DEG C of refrigerators, save backup,
The making of step 5 environmental protection mixed feed
Benthic diatom algae cream 35 parts, 1.5 parts, sheep excrement, 0.5 part, rabbit excrement, Swine blood meal 0.2 part, dusty yeast 0.4 part, maize alcohol stillage 2.4 parts, 0.3 part, p.malaianus powder, 0.7 part, water caltrop powder, 0.5 part, hydrilla verticillata powder, animal tankage 0.1 part, earthworm powder 0.8 part, fly maggot powder 0.5 part, artemia powder 0.3 part, Kelp Powder 1.2 parts, 0.2 part, garlic powder, methionine 1.4 parts, lysine 0.7 part, dehydrated potato powder 5 parts, 1 part, locust powder, alfalfa meal 3 parts, 0.8 part, clam worm powder, trash fish 1.4 parts, snail shell digested tankage 2.4 parts; Often kind of raw material dries or 65 DEG C of drying machine dryings, and break into powdery, mixing and blending machine fully stirs, mix, environmental protection bait completes;
Step 6 mixed feed feeding method
When throwing something and feeding, get a Plastic Drum, add mixed feed, what add 25 times (weight ratios) again boils cooled seawater, be stirred well to evenly, make scattered paste shape, evenly can splash towards the Zhong Quan pond, pond of cultivating pen shell, early, middle and late each 1 time of every day, each feeding volume accounts for 0.2% of pen shell gross weight by bait weight.
Industrial ammonia in step 1 in nutrient solution formula, cow dung leachate, human urine, peptide urea, ammonium sulfate etc. will add by the order provided in formula, to avoid chemical reaction;
Various inorganic nutrient salts in step 1 in nutrient solution formula can be made into mother liquor in advance and be placed in 4 DEG C of refrigerator cold-storages and save backup, and do not exceed 30 days standing time;
Described in step 1, people urinates preparation method: get people and urinate 1000 milliliters, after conical flask boils 5 minutes, cools for subsequent use;
The leachate of cow dung described in step 1 preparation method: get cow dung 50 kilograms, add 3 kilograms, wheat bran, corn flour 1 kilogram, 4 liters, running water, abundant stirring, pile coniform, spontaneous fermentation 15 days, rear stand straticulation, be exposed to the sun under sunlight 3 days, after get 3 kilograms and put into bucket, add 10 liters of distilled water, fully stir evenly, leave standstill, 100 order silk cover filterings after 24 hours, waste, get filtrate heating, boil 45 minutes, kill bacterium wherein, mould, virus etc., cooling, precipitate 12 hours, abandon sediment, get supernatant, obtain cow dung leachate, load grinding port plug reagent bottle, sealing, be placed in 4 DEG C of refrigerators for subsequent use,
The preparation method of soil extract described in step 1: get the woods 1000 parts, soil at the middle and upper levels, adding distil water 2000 parts, then add NaOH 2.5 parts, heating, boils 45 minutes, cooling, precipitation 12 hours, and get supernatant, Filter paper filtering, filtrate is soil extract;
Ooze described in step 1 extracts liquid and preparation method thereof: get the more fertile ooze that what is not black again, add 2 parts of fresh water, stir with 1 part of mud, leave standstill 1-2 minute, get upper strata mud in aluminum pot, the amount adding 1 gram of NaOH by every 1000 milliliters of mud adds NaOH, boils 25 minutes.Constantly need stir when boiling, boil rear cooling, leave standstill that within 24 hours, to get supernatant for subsequent use;
Ammonium sulfate described in step 1, ammonium nitrate, potassium sulfate, potassium chloride, IBDU, oxamides, ammonium magnesium phosphate, APP, calcium polyphosphate potassium, the chemicals purity such as sodium acid carbonate all need chemical pure rank;
The composition of Chinese herbal medicine described in step 1 and mass fraction are: the bighead atractylodes rhizome 10 parts, Radix Codonopsis 8 parts, northern stilbene 5 parts, 5 parts, mountain, Huaihe River, glossy ganoderma 5 parts, 10 parts, Radix Glycyrrhizae, tarragon 30 parts, Fructus Aurantii 12 parts, Divine Comedy 21 parts, Radix Codonopsis 33 parts, the tuber of dwarf lilyturf 13 parts, radix scrophulariae 22 parts, the coptis 5 parts, cape jasmine 15 parts, the root of large-flowered skullcap 5 parts, 10 parts, mulberry leaf, golden cypress 7 parts, the root bark of white mulberry 10 parts, the radix paeoniae rubrathe 20 parts, the capsule of weeping forsythia 14 parts, matrimony vine 1 part, distilled water 150 parts; First soak 60 minutes, decoct with marmite, big fire (intense fire) is heated, and boiled rear little fire (slow fire) is boiled after 25 minutes and stopped heating, cool, leave standstill 6 hours, get supernatant 100 order silk cover filterings, waste, obtain composite Chinese herbal liquid, 4 DEG C of Refrigerator stores, for subsequent use;
Cultivate diatom magnetized drinking water preparation method used in step 1: after seawater boils, cooling, after flowing through water magnetizing appts, is magnetized drinking water.Water magnetizing appts magnetic induction intensity is 0.3T, and flow is 2t/h, and flow velocity is 0.3m/s;
Cultivate in step 3 inside when benthic diatom fishbowl used is bought and with some ornamental pasture and water, stone, shell, artificial hillock etc., may be removed, with increase Water space, handled easily and avoid pollute;
The excrement of sheep described in step 5, rabbit excrement need at the 5-7 days that is exposed to the sun in the sun, and rear pulverizer breaks into powder; In step 5, all raw materials must break into powder after dry (drying or 65 DEG C of drying machine dryings), and powder diameter is less than 40 microns;
Described in step 2 and 3, benthic diatom mainly refers to: little shape boat-shaped algae, the two eyebrow algae of coffee, Navichla corymbosa, half-naked boat-shaped algae, variable two eyebrow algae eye mutation, the mutation of two sharp quarrel algae thin head, two water chestnut seam boat algae, rhombus algae etc.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (1)
1. a preparation method for pinniform pen shell environmental protection bait, is characterized in that step is as follows:
The preparation of step 1 benthic diatom nutrient solution
Nutrient solution formula (by weight): containing the industrial ammonia 1.2 parts of ammonia 25%-28%, cow dung leachate 1 part, people urinates liquid 0.4 part, peptide urea 0.1 part, 0.03 part, ammonium sulfate, 0.2 part, ammonium nitrate, potassium sulfate 0.2 part, 0.5 part, potassium chloride, IBDU 0.1 part, oxamides 0.2 part, ammonium magnesium phosphate 0.05 part, APP 0.08 part, 0.02 part, calcium polyphosphate potassium, sodium metasilicate 0.6 part, herbal liquid medicine 1.2 parts, polyamines 0.001 part, abscisin 0.004 part, sodium acid carbonate 3 parts, soil extract 1.2 parts, ooze extract 1 part, Sodium Benzoate 0.02 part, potassium sorbate 0.002 part, plant ash 0.4 part, scalding and magnetization after 1000 parts, clean seawater, abundant mixing, stir after, the acetic acid solution (volume ratio) with 15% and sodium acid carbonate (weight ratio) adjust ph of 20% are to 7.5-7.8,
The collection of step 2 benthic diatom
Get fortune windmillpalm sheath-fibre, be bundled into bundle, running water is cleaned, be placed in the Porcelain Jar of the liquor potassic permanganate filling 80ppm and soak 8 hours, rinse with running water, hang over that coastal waters (bank 300-5000 rice off sea) sea-tangle is floating to be cut down, in underwater, 0.5-2 rice is dark, hang 7-10 days, namely fortune windmillpalm sheath-fibre attached to a large amount of benthic diatom, as the algae kind that next step is cultivated, be brought up, put into and fill in the Plastic Drum of ordinary sea water, for subsequent use;
The cultivation of step 3 benthic diatom
Get a simple glass goldfish jar, cuboid, volume 200 liters, wash, with the liquor potassic permanganate soaking disinfection 5 hours of 80ppm, outwell, with distilled water flushing 3 times, add nutrient solution described in step 1 120 liters, the fortune windmillpalm sheath-fibre 3-4 being attached with benthic diatom put into described in step 2 restraints, and goldfish jar mouth is put a batten, with cord, fortune windmillpalm sheath-fibre be suspended in fish jar instead of sink at the bottom of fish jar, air-charging incubation, aeration quantity should not be too large, allows frustule float and not sink.Intensity of illumination 600-1200lux, illumination should not be too strong, otherwise green algae is understood amount reproduction thus suppresses the growth of diatom; Fish jar covering suitable for reading one block of glass, the gas overflowed in nutrient solution can distribute from batten; Glass jar and glass above, coated with the plastic sheeting of green, irradiate the spectrum mainly green light into algae liquid like this, are conducive to synthesis and the accumulation of the high unsaturated fatty acids such as algae EPA, DHA; Glass jar is placed in microdisk electrode room and cultivates, air-conditioning temperature control, and fluorescent tube irradiates, first 5 days of cultivation, control temperature 18 DEG C, intensity of illumination 600-900lux, light dark period L: D=16h: 8h, namely every day 16 h light, 8 h dark; Cultivate 6-9 days, adjust the temperature to 21 DEG C, intensity of illumination 900-1200lux, light dark period L: D=20h: 4h, namely every day 20 h light, 4 h dark.Be cultured to the 6th day, add the nutrient solution 10 liters described in step 1, with the consumption of the salt that supplements the nutrients, be cultured to the 7th day, add distilled water 5 liters, to dilute algae liquid and to supplement the moisture vapored away; Be cultured to the 9th day, diatom density can reach 1,200 ten thousand milliliters, stops cultivating, and can collect frustule;
The collection of step 4 benthic diatom
Get poly-phosphorus iron chloride (PPFC), poly-phosphorus aluminium chloride (PPAC) and ferric-polysilicate (PSF) (commercially available), in poly-phosphorus iron chloride: poly-phosphorus aluminium chloride: ferric-polysilicate=1: the ratio (weight ratio) of 2: 0.6 fully mixes, make compound precipitants, the amount adding 120 milligrams by often liter of algae liquid adds compound precipitants, stir, leave standstill after 36 hours, most frustule sinks to bottom, abandon supernatant, bottom yellow green toothpaste-like diatom algae cream can be obtained, collect, load beaker, preservative film seals, be placed in-4 DEG C of refrigerators, save backup,
The making of step 5 environmental protection mixed feed
Benthic diatom algae cream 35 parts, 1.5 parts, sheep excrement, 0.5 part, rabbit excrement, Swine blood meal 0.2 part, dusty yeast 0.4 part, maize alcohol stillage 2.4 parts, 0.3 part, p.malaianus powder, 0.7 part, water caltrop powder, 0.5 part, hydrilla verticillata powder, animal tankage 0.1 part, earthworm powder 0.8 part, fly maggot powder 0.5 part, artemia powder 0.3 part, Kelp Powder 1.2 parts, 0.2 part, garlic powder, methionine 1.4 parts, lysine 0.7 part, dehydrated potato powder 5 parts, 1 part, locust powder, alfalfa meal 3 parts, 0.8 part, clam worm powder, trash fish 1.4 parts, snail shell digested tankage 2.4 parts; Often kind of raw material dries or 65 DEG C of drying machine dryings, and break into powdery, mixing and blending machine fully stirs, mix, environmental protection bait completes;
Step 6 mixed feed feeding method
When throwing something and feeding, get a Plastic Drum, add mixed feed, what add 25 times (weight ratios) again boils cooled seawater, be stirred well to evenly, make scattered paste shape, evenly can splash towards the Zhong Quan pond, pond of cultivating pen shell, early, middle and late each 1 time of every day, each feeding volume accounts for 0.2% of pen shell gross weight by bait weight.
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| CN108668959A (en) * | 2018-03-30 | 2018-10-19 | 镇沅今晨山水产科技有限公司 | A kind of artificial breeding method of great Kong Select-Committee |
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