CN104782576A - Method for breeding calanus finmarchicus with porcelain jar - Google Patents
Method for breeding calanus finmarchicus with porcelain jar Download PDFInfo
- Publication number
- CN104782576A CN104782576A CN201510173133.3A CN201510173133A CN104782576A CN 104782576 A CN104782576 A CN 104782576A CN 201510173133 A CN201510173133 A CN 201510173133A CN 104782576 A CN104782576 A CN 104782576A
- Authority
- CN
- China
- Prior art keywords
- bottle
- algae
- liquid
- wise man
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Mushroom Cultivation (AREA)
- Cultivation Of Seaweed (AREA)
Abstract
The invention discloses a method for breeding calanus finmarchicus with a procelin jar and belongs to the technical field of aquaculture. In the prior art, an outdoor extensive mode and an indoor intensive mode are used for breeding calanus finmarchicus; an outdoor earth pond mode is low in breeding cost but low in yield, the outdoor earth pond mode is limited by the seasons and water temperature, and the calanus finmarchicus is prone to pollution of viruses and bacteria; the intensive breeding mode is high in yield and scarcely influenced by natural conditions, but the cost is high. Compared with the prior art, the method has the advantages that the porcelain jar is firm, durable, not prone to breakage, convenient to clean and capable of being repeatedly used, operation is convenient, and investment is saved; the calanus finmarchicus is fed with germs, cyclotella, skeletonema costatum and rhombus thalassiosira, the calanus finmarchicus grows rapidly, propagates rapidly and is robust and high in yield, and the limitation of the seasons is avoided.
Description
Technical field the invention belongs to aquaculture field, particularly relates to a kind of method that Porcelain Jar cultivates flying horse wise man water flea.
Background technology
Flying horse wise man water flea Calanus fimarchicus is a kind of small ocean copepods zooplankton, be subordinate to Arthropoda Arthropoda, Crustachia Crustacea, calanoida Calanus, wise man water flea section Calanidae, wise Daphnia Calanus, body is long is less than 3mm, battalion swims and parasitic life, is distributed in ocean, warm temperate zone.Being widely distributed in Bohai and Yellow Seas and the East Sea, is the important bait of economic fish such as Pacific herring, chub mackerel and the young, baleen whales, European North Sea Pacific herring.Flying horse wise man is directly fished in Norway coastal waters, and water flea does the mankind, livestock and poultry foodstuff has history decades, has very high nutritive value.In addition, flying horse wise man water flea and ocean current closely related, can be used as ocean current, water body indicator organism; Also can be used as water pollution indicator organism also as the mark finding fishing ground.In filtered water, the suspended particulate such as diatom, bacterium, organic debris is food.
Current copepoda is cultivated outdoor extensive style and indoor intensiveization two kinds.The outdoor extensive cultivation place of large area should be selected in bay, river mouth and nearshore waters.Outside scenery output height often depends on bait micro-algae supply situation, and outdoor earth pond is cultivated copepoda and achieved good result in recent years, but remain unstable in output, continue that supply time is short, the problem such as susceptible viral and germ contamination.From the nineties in last century, people attempt carrying out the intensive cultivation of copepoda.Wise man's water flea class and violent some kind of water flea class, comparatively strong and as the ideal object of intensive cultivation to environmental suitability, for wise water flea kind many genus offshore or the river mouth kind of intensive cultivation, as Acartia genus, chest thorn Daphnia, very wide Daphnia and wide Daphnia, these kind individualities are little, breeding is fast, wide temperature, wide salt, are applicable to laboratory cultures.Relative to wise water flea class, the violent water flea class battalion end dwell life, can High Density Cultivation, the history of life is short, bait is extensive etc., and feature makes it be suitable for intensive large-scale culture.Outdoor earth pond cultivation fee is low, but yields poorly, by limiting and susceptible viral and germ contamination in season and water temperature; Intensive cultivation output is high, little by effect of natural conditions, but cost is high, and for winter and early nursery in spring, the intensive cultivation of copepoda is more applicable.
About the cultivation of flying horse wise man water flea, past Zhao Wen etc. has invented a kind of large-scale cultivation method for marine floating harpacticoida (application number CN200910248644).This invention can improve cultivation efficiency, and reduce production cost, the nursery for marine products economic animal provides reliable food supply.Described violent water flea large-scale cultivation method carries out as follows: violent water flea cultivation salinity water is 20ppt, temperature 24 DEG C, water body dissolved oxygen > 5mg/l, pH=7-8, bait is at least one in lsochrysis zhanjiangensis, Dunaliella salina and Chaetoceros muelleri, and the initial density of violent water flea cultivation is 50-100ind./l breeding water body, and the quantity delivered of bait is 5 ~ 10l/d, upgrade 300l breeding water every day, cultivation to violent water flea density is at least 1500ind./ml; Gather in the crops the violent water flea in 500l water body every day and fill into fresh breeding water, bait consumption is 10-15l/d, continuous culture 30-50d.The method easily pollutes, and power consumption is higher and operating technology requires high, and general workman is difficult to grasp, and practicality is not strong.
In order to overcome the deficiency of current technology, the invention discloses a kind of method that Porcelain Jar cultivates flying horse wise man water flea, compared with conventional art, Heterosis of the present invention exists: 1) adopt Porcelain Jar, sturdy and durable, not easily break up, scrub conveniently, reuse, easy to operate, economize on the use of funds; 2) throw something and feed bacterium and little ring algae, Skeletonema Costatum, rhombus hailian seaweed, flying horse wise man water flea growth and breeding is fast, and physique is healthy and strong, and output is high, is not subject to seasonal restrictions; 3) inflate and use magnetized drinking water with conical flask series connection when cultivating micro-algae, algal grown is fast, and output is high, and the gas be filled with obtains 3 times and utilizes, and has saved resource and energy.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides the cultural method that a kind of Porcelain Jar cultivates flying horse wise man water flea, step is as follows:
Flying horse wise man water flea bait comprises micro-algae, bacterium liquid;
The preparation of step 1 flying horse wise man water flea bait micro-algae
Micro algae culturing liquid is filled a prescription as follows by ratio of weight and the number of copies: 0.4 part, ammonium nitrate, ammonium chloride 0.2 part, diammonium phosphate 0.06 part, double superphosphate 0.1 part, isobutyl fork bishydrazicarbonyl 0.08 part, sodium bicarbonate 1.5 parts, potassium sulfate 0.5 part, zinc chloride 0.002 part, ammonium molybdate 0.0001 part, abscisic acid 0.00001 part, borax 0.03 point, 0.0004 part, copper sulphate, sodium silicate 0.1 part, Fe-EDTA 0.00005 part, people urinates 1.2 parts, boils cooled Magnetized Seawater 1000 parts, get the conical flask of 3 5000 milliliters, wash with test-tube brush, then use distilled water flushing 3 times, rubber stopper is stamped 2 holes, insert 2 glass tubes, through 5-6 millimeter in glass tube, one longer, is air inlet pipe, be inserted into close at the bottom of bottle, another root is shorter, and be escape pipe, rubber stopper 3-5 centimetre is exposed at two ends, the gas tube of second bottle is connected with softer rubber tube or emulsion tube with the blast pipe of first bottle, and the gas be filled with like this can obtain 3 times and utilize, each bottle adds micro algae culturing liquid 2500 milliliters, get purebred, growth is active, pollution-free, the little ring algae Cyclotella spp. of exponential phase of growth, Skeletonema Costatum Skeletonema costatum, rhombus hailian seaweed Thalassionema nitzschioides inoculates respectively, carry out monoculture, namely first bottle (A bottle) inoculates little ring algae, inoculum density 50,000 cells/ml, second bottle (B bottle) inoculates Skeletonema Costatum, inoculum density 40,000 cells/ml, 3rd bottle (C bottle) inoculates rhombus hailian seaweed, inoculum density 80,000 cells/ml, these 3 flasks are put into microdisk electrode room to cultivate, first bottle (A bottle) gas tube upper end is connected with air compressor machine by softer plastic tube or emulsion tube, air-charging incubation, aeration quantity 2-3 liter/min, make cultivation algae liquid keep higher dissolved oxygen and make frustule suspend not sink, microdisk electrode room air-conditioning temperature control, common energy-saving lamp provides illumination, Dual culture 12 days, first 4 days of cultivation, adjust the temperature to 20-22 DEG C, intensity of illumination 2000-2500lux, the 5-8 days of cultivation, adjusts the temperature to 24-26 DEG C, intensity of illumination 4000-4500lux, the 9-12 days cultivated, adjusts the temperature to 26-28 DEG C, intensity of illumination 6000-7000lux, be cultured to the 7th day, each bottle is added 100 milliliters of fresh mediums to supplement used up nutritive salt, the 8th day, each bottle of distilled water added after 50 milliliters of magnetization, to supplement because inflating the moisture that vapors away and diluting algae liquid, be cultured to the 12nd day, little ring algae density can reach 3,500,000 cells/ml, Skeletonema Costatum density can reach 2,400,000 cells/ml, rhombus hailian seaweed density can reach 4,500,000 cells/ml, stop cultivating, pull out pipe, algae liquid is kept in ventilation, dark, 4 DEG C of environment, be prepared for flying horse wise man water flea of throwing something and feeding,
The preparation of step 2 bacterium liquid
Inoculum is filled a prescription as follows by ratio of weight and the number of copies: fermentation cow dung 1 part, fermented rabbit dung 0.8 part, 0.5 part, Chinese cabbage leaf, peanut seedling 1.5 parts, 0.6 part, straw leaf, soya-bean cake 0.4 part, groundnut cake 0.8 part, 0.2 part, wheat bran, corn flour 0.1 part, boils cooling then 1000 parts, magnetized seawater; Put in Plastic Drum, room temperature 18-28 DEG C is soaked 96 hours, period stirred every 8 hours, stir once, finally solid content pulled out and abandon, remaining liquid leaves standstill, precipitating 24 hours, waste dregs, supernatant 100 order silk cover filterings, waste again, filtrate is bacterium liquid, prepares flying horse wise man water flea of throwing something and feeding;
The cultivation of step 3 flying horse wise man water flea
Get the Porcelain Jar that a bite volume is 100 liters, wash, add and boil cooled Magnetized Seawater 40 liters, add bacterium liquid described in step 2 10 liters, stir, get health, the inoculation of active, purebred flying horse wise man water flea, inoculum density 25-40/liter, stir; Insert plastics or rubber tube inflation in cylinder, the lower end of pipe connects a gas exhausting stone, and the upper end of pipe is connected with air compressor machine, air-charging incubation, and aeration quantity is 8-9 liter/min; This cylinder is put into indoor, air-conditioning temperature control, temperature is set to 23-27 DEG C, arranges an electricity-saving lamp above cylinder, intensity of illumination is set to 1500-2500lux, and from the 7th day, every day supplemented bacterium liquid 0.5 liter of throwing something and feeding, little ring algae liquid 1 liter, Skeletonema Costatum liquid 2 liters, rhombus hailian seaweed liquid 1 liter; From the 9th day, change water with boiling cooled Magnetized Seawater, every day, quantity of exchanged water was 1/5 of total Water, the silk cover filtering using mesh to be less than 80 microns when changing water, in case flying horse wise man water flea outflows with water; From the 30th day, gather flying horse wise man water flea once every 5 days, the amount of gathering is 1/3 of flying horse wise man water flea standing crop, and collecting method is the silk cover filtering of 60-100 micron with mesh, then supplements throw something and feed bacterium liquid and algae liquid, continuation cultivation; Cultivate by 60 days, flying horse wise man water flea density can reach 1500-2500/liter, stops cultivating, once all gathers, cultivate complete.
Beneficial outcomes is in order to overcome the deficiency of current technology, and the invention discloses a kind of method that Porcelain Jar cultivates flying horse wise man water flea, compared with conventional art, Heterosis of the present invention exists: 1) adopt Porcelain Jar, sturdy and durable, not easily break up, scrub conveniently, reuse, easy to operate, economize on the use of funds; 2) throw something and feed bacterium and little ring algae, Skeletonema Costatum, rhombus hailian seaweed, flying horse wise man water flea growth and breeding is fast, and physique is healthy and strong, and output is high, is not subject to seasonal restrictions; 3) inflate and use magnetized drinking water with conical flask series connection when cultivating micro-algae, algal grown is fast, and output is high, and the gas be filled with obtains 3 times and utilizes, and has saved resource and energy.
Accompanying drawing explanation
Fig. 1 flying horse wise man water flea form;
Bait micro-algae method is cultivated in Fig. 2 conical flask series connection; Arrow indication is charge air conditioning trend; Wherein little ring algae cultivated by A bottle, and Skeletonema Costatum cultivated by B bottle, and rhombus hailian seaweed cultivated by C bottle;
Fig. 3 cultivates flying horse wise man water flea Porcelain Jar used.
Embodiment
Porcelain Jar cultivates a cultural method for flying horse wise man water flea, and step is as follows:
Flying horse wise man water flea bait comprises micro-algae, bacterium liquid;
The preparation of step 1 flying horse wise man water flea bait micro-algae
Micro algae culturing liquid is filled a prescription as follows by ratio of weight and the number of copies: 0.4 part, ammonium nitrate, ammonium chloride 0.2 part, diammonium phosphate 0.06 part, double superphosphate 0.1 part, isobutyl fork bishydrazicarbonyl 0.08 part, sodium bicarbonate 1.5 parts, potassium sulfate 0.5 part, zinc chloride 0.002 part, ammonium molybdate 0.0001 part, abscisic acid 0.00001 part, borax 0.03 point, 0.0004 part, copper sulphate, sodium silicate 0.1 part, Fe-EDTA 0.00005 part, people urinates 1.2 parts, boils cooled Magnetized Seawater 1000 parts, get the conical flask of 3 5000 milliliters, wash with test-tube brush, then use distilled water flushing 3 times, rubber stopper is stamped 2 holes, insert 2 glass tubes, through 5-6 millimeter in glass tube, one longer, is air inlet pipe, be inserted into close at the bottom of bottle, another root is shorter, and be escape pipe, rubber stopper 3-5 centimetre is exposed at two ends, the gas tube of second bottle is connected with softer rubber tube or emulsion tube with the blast pipe of first bottle, and the gas be filled with like this can obtain 3 times and utilize, each bottle adds micro algae culturing liquid 2500 milliliters, get purebred, growth is active, pollution-free, the little ring algae Cyclotella spp. of exponential phase of growth, Skeletonema Costatum Skeletonema costatum, rhombus hailian seaweed Thalassionema nitzschioides inoculates respectively, carry out monoculture, namely first bottle (A bottle) inoculates little ring algae, inoculum density 50,000 cells/ml, second bottle (B bottle) inoculates Skeletonema Costatum, inoculum density 40,000 cells/ml, 3rd bottle (C bottle) inoculates rhombus hailian seaweed, inoculum density 80,000 cells/ml, these 3 flasks are put into microdisk electrode room to cultivate, first bottle (A bottle) gas tube upper end is connected with air compressor machine by softer plastic tube or emulsion tube, air-charging incubation, aeration quantity 2-3 liter/min, make cultivation algae liquid keep higher dissolved oxygen and make frustule suspend not sink, microdisk electrode room air-conditioning temperature control, common energy-saving lamp provides illumination, Dual culture 12 days, first 4 days of cultivation, adjust the temperature to 20-22 DEG C, intensity of illumination 2000-2500lux, the 5-8 days of cultivation, adjusts the temperature to 24-26 DEG C, intensity of illumination 4000-4500lux, the 9-12 days cultivated, adjusts the temperature to 26-28 DEG C, intensity of illumination 6000-7000lux, be cultured to the 7th day, each bottle is added 100 milliliters of fresh mediums to supplement used up nutritive salt, the 8th day, each bottle of distilled water added after 50 milliliters of magnetization, to supplement because inflating the moisture that vapors away and diluting algae liquid, be cultured to the 12nd day, little ring algae density can reach 3,500,000 cells/ml, Skeletonema Costatum density can reach 2,400,000 cells/ml, rhombus hailian seaweed density can reach 4,500,000 cells/ml, stop cultivating, pull out pipe, algae liquid is kept in ventilation, dark, 4 DEG C of environment, be prepared for flying horse wise man water flea of throwing something and feeding,
The preparation of step 2 bacterium liquid
Inoculum is filled a prescription as follows by ratio of weight and the number of copies: fermentation cow dung 1 part, fermented rabbit dung 0.8 part, 0.5 part, Chinese cabbage leaf, peanut seedling 1.5 parts, 0.6 part, straw leaf, soya-bean cake 0.4 part, groundnut cake 0.8 part, 0.2 part, wheat bran, corn flour 0.1 part, boils cooling then 1000 parts, magnetized seawater; Put in Plastic Drum, room temperature 18-28 DEG C is soaked 96 hours, period stirred every 8 hours, stir once, finally solid content pulled out and abandon, remaining liquid leaves standstill, precipitating 24 hours, waste dregs, supernatant 100 order silk cover filterings, waste again, filtrate is bacterium liquid, prepares flying horse wise man water flea of throwing something and feeding;
The cultivation of step 3 flying horse wise man water flea
Get the Porcelain Jar that a bite volume is 100 liters, wash, add and boil cooled Magnetized Seawater 40 liters, add bacterium liquid described in step 2 10 liters, stir, get health, the inoculation of active, purebred flying horse wise man water flea, inoculum density 25-40/liter, stir; Insert plastics or rubber tube inflation in cylinder, the lower end of pipe connects a gas exhausting stone, and the upper end of pipe is connected with air compressor machine, air-charging incubation, and aeration quantity is 8-9 liter/min; This cylinder is put into indoor, air-conditioning temperature control, temperature is set to 23-27 DEG C, arranges an electricity-saving lamp above cylinder, intensity of illumination is set to 1500-2500lux, and from the 7th day, every day supplemented bacterium liquid 0.5 liter of throwing something and feeding, little ring algae liquid 1 liter, Skeletonema Costatum liquid 2 liters, rhombus hailian seaweed liquid 1 liter; From the 9th day, change water with boiling cooled Magnetized Seawater, every day, quantity of exchanged water was 1/5 of total Water, the silk cover filtering using mesh to be less than 80 microns when changing water, in case flying horse wise man water flea outflows with water; From the 30th day, gather flying horse wise man water flea once every 5 days, the amount of gathering is 1/3 of flying horse wise man water flea standing crop, and collecting method is the silk cover filtering of 60-100 micron with mesh, then supplements throw something and feed bacterium liquid and algae liquid, continuation cultivation; Cultivate by 60 days, flying horse wise man water flea density can reach 1500-2500/liter, stops cultivating, once all gathers, cultivate complete.
People described in step 1 urinates preparation method and gets people and urinate 1000 milliliters, loads after conical flask boils 5 minutes, cools for subsequent use;
Ammonium nitrate described in step 1, ammonium chloride, diammonium phosphate, double superphosphate, isobutyl fork bishydrazicarbonyl, sodium bicarbonate, potassium sulfate, zinc chloride, ammonium molybdate, abscisic acid, borax, copper sulphate, sodium silicate, Fe-EDTA chemicals purity all needs chemical pure rank;
Described in step 1,2 and 3, magnetized drinking water preparation method ordinary sea water filters through absorbent cotton, and after boiling, cooling, after flowing through water magnetizing appts, is Magnetized Seawater.Water magnetizing appts magnetic induction intensity is 0.3T, and flow is 3t/h, and flow velocity is 0.2m/s.
The cow dung of ferment described in step 2 preparation method gets cow dung 50 kilograms, adds 3 kilograms, wheat bran, corn flour 1 kilogram, and 4 liters, running water, fully stirs, and piles coniform, spontaneous fermentation 15 days, and rear stand straticulation is exposed to the sun under sunlight 3 days, cow dung of must fermenting;
The rabbit of ferment described in step 2 excrement preparation method presses the part by weight mixing of 60: 10: 12: 15: 2: 1 dry rabbit excrement, green grass, soya-bean cake, rice bran, wheat bran and humus soil, stir, load airtight in cylinder fermentation, after 30 days, pour out, at clean water mire, stand straticulation, sun 72 hours, obtains fermented rabbit dung;
The leaf of Chinese cabbage described in step 2, peanut seedling, straw leaf will be cut into 1-3 centimeter length, to facilitate dip operation.
Last it is noted that above embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to previous embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in previous embodiment, or carries out equivalent replacement to wherein portion of techniques feature; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.
Claims (1)
1. cultivate a cultural method for flying horse wise man water flea with Porcelain Jar, it is characterized in that:
Flying horse wise man water flea bait comprises micro-algae, bacterium liquid;
The preparation of step 1 flying horse wise man water flea bait micro-algae
Micro algae culturing liquid is filled a prescription as follows by ratio of weight and the number of copies: 0.4 part, ammonium nitrate, ammonium chloride 0.2 part, diammonium phosphate 0.06 part, double superphosphate 0.1 part, isobutyl fork bishydrazicarbonyl 0.08 part, sodium bicarbonate 1.5 parts, potassium sulfate 0.5 part, zinc chloride 0.002 part, ammonium molybdate 0.0001 part, abscisic acid 0.00001 part, borax 0.03 point, 0.0004 part, copper sulphate, sodium silicate 0.1 part, Fe-EDTA 0.00005 part, people urinates 1.2 parts, boils cooled Magnetized Seawater 1000 parts, get the conical flask of 3 5000 milliliters, wash with test-tube brush, then use distilled water flushing 3 times, rubber stopper is stamped 2 holes, insert 2 glass tubes, through 5-6 millimeter in glass tube, one longer, is air inlet pipe, be inserted into close at the bottom of bottle, another root is shorter, and be escape pipe, rubber stopper 3-5 centimetre is exposed at two ends, the gas tube of second bottle is connected with softer rubber tube or emulsion tube with the blast pipe of first bottle, and the gas be filled with like this can obtain 3 times and utilize, each bottle adds micro algae culturing liquid 2500 milliliters, get purebred, growth is active, pollution-free, the little ring algae Cyclotella spp. of exponential phase of growth, Skeletonema Costatum Skeletonema costatum, rhombus hailian seaweed Thalassionema nitzschioides inoculates respectively, carry out monoculture, namely first bottle (A bottle) inoculates little ring algae, inoculum density 50,000 cells/ml, second bottle (B bottle) inoculates Skeletonema Costatum, inoculum density 40,000 cells/ml, 3rd bottle (C bottle) inoculates rhombus hailian seaweed, inoculum density 80,000 cells/ml, these 3 flasks are put into microdisk electrode room to cultivate, first bottle (A bottle) gas tube upper end is connected with air compressor machine by softer plastic tube or emulsion tube, air-charging incubation, aeration quantity 2-3 liter/min, make cultivation algae liquid keep higher dissolved oxygen and make frustule suspend not sink, microdisk electrode room air-conditioning temperature control, common energy-saving lamp provides illumination, Dual culture 12 days, first 4 days of cultivation, adjust the temperature to 20-22 DEG C, intensity of illumination 2000-2500lux, the 5-8 days of cultivation, adjusts the temperature to 24-26 DEG C, intensity of illumination 4000-4500lux, the 9-12 days cultivated, adjusts the temperature to 26-28 DEG C, intensity of illumination 6000-7000lux, be cultured to the 7th day, each bottle is added 100 milliliters of fresh mediums to supplement used up nutritive salt, the 8th day, each bottle of distilled water added after 50 milliliters of magnetization, to supplement because inflating the moisture that vapors away and diluting algae liquid, be cultured to the 12nd day, little ring algae density can reach 3,500,000 cells/ml, Skeletonema Costatum density can reach 2,400,000 cells/ml, rhombus hailian seaweed density can reach 4,500,000 cells/ml, stop cultivating, pull out pipe, algae liquid is kept in ventilation, dark, 4 DEG C of environment, be prepared for flying horse wise man water flea of throwing something and feeding,
The preparation of step 2 bacterium liquid
Inoculum is filled a prescription as follows by ratio of weight and the number of copies: fermentation cow dung 1 part, fermented rabbit dung 0.8 part, 0.5 part, Chinese cabbage leaf, peanut seedling 1.5 parts, 0.6 part, straw leaf, soya-bean cake 0.4 part, groundnut cake 0.8 part, 0.2 part, wheat bran, corn flour 0.1 part, boils cooling then 1000 parts, magnetized seawater; Put in Plastic Drum, room temperature 18-28 DEG C is soaked 96 hours, period stirred every 8 hours, stir once, finally solid content pulled out and abandon, remaining liquid leaves standstill, precipitating 24 hours, waste dregs, supernatant 100 order silk cover filterings, waste again, filtrate is bacterium liquid, prepares flying horse wise man water flea of throwing something and feeding;
The cultivation of step 3 flying horse wise man water flea
Get the Porcelain Jar that a bite volume is 100 liters, wash, add and boil cooled Magnetized Seawater 40 liters, add bacterium liquid described in step 2 10 liters, stir, get health, the inoculation of active, purebred flying horse wise man water flea, inoculum density 25-40/liter, stir; Insert plastics or rubber tube inflation in cylinder, the lower end of pipe connects a gas exhausting stone, and the upper end of pipe is connected with air compressor machine, air-charging incubation, and aeration quantity is 8-9 liter/min; This cylinder is put into indoor, air-conditioning temperature control, temperature is set to 23-27 DEG C, arranges an electricity-saving lamp above cylinder, intensity of illumination is set to 1500-2500lux, and from the 7th day, every day supplemented bacterium liquid 0.5 liter of throwing something and feeding, little ring algae liquid 1 liter, Skeletonema Costatum liquid 2 liters, rhombus hailian seaweed liquid 1 liter; From the 9th day, change water with boiling cooled Magnetized Seawater, every day, quantity of exchanged water was 1/5 of total Water, the silk cover filtering using mesh to be less than 80 microns when changing water, in case flying horse wise man water flea outflows with water; From the 30th day, gather flying horse wise man water flea once every 5 days, the amount of gathering is 1/3 of flying horse wise man water flea standing crop, and collecting method is the silk cover filtering of 60-100 micron with mesh, then supplements throw something and feed bacterium liquid and algae liquid, continuation cultivation; Cultivate by 60 days, flying horse wise man water flea density can reach 1500-2500/liter, stops cultivating, once all gathers, cultivate complete.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173133.3A CN104782576B (en) | 2015-04-13 | 2015-04-13 | A kind of cultural method with the wise water flea of Porcelain Jar culture flying horse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510173133.3A CN104782576B (en) | 2015-04-13 | 2015-04-13 | A kind of cultural method with the wise water flea of Porcelain Jar culture flying horse |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104782576A true CN104782576A (en) | 2015-07-22 |
| CN104782576B CN104782576B (en) | 2017-09-29 |
Family
ID=53548136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510173133.3A Expired - Fee Related CN104782576B (en) | 2015-04-13 | 2015-04-13 | A kind of cultural method with the wise water flea of Porcelain Jar culture flying horse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104782576B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996365A (en) * | 2015-08-04 | 2015-10-28 | 厦门大学 | Screening and cultural method for initial feed bestiolina similis of ocean fancy fishes |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1405749A1 (en) * | 1986-08-04 | 1988-06-30 | Азовский научно-исследовательский институт рыбного хозяйства | Method of storing the eggs of rotizera at rest, brashiouus calyciflorous calycyflorus pallas |
| CN101731185A (en) * | 2009-12-23 | 2010-06-16 | 大连水产学院 | Large-scale cultivation method for marine floating harpacticoida |
| CN202588097U (en) * | 2012-04-09 | 2012-12-12 | 上海海洋大学 | Water flow buffering device |
| CN102948399A (en) * | 2012-04-13 | 2013-03-06 | 环境保护部南京环境科学研究所 | Daphnia magna breeding method for evaluating safety of transgenic rice |
| CN103834569A (en) * | 2014-03-03 | 2014-06-04 | 临沂大学 | Microalga concentration culture solution and application thereof |
| CN103966100A (en) * | 2014-05-26 | 2014-08-06 | 临沂大学 | Culture medium and culture method for culturing isochrysis galbana and heterogloea sp. by use of slaughtering plant wastewater |
| CN104212718A (en) * | 2014-09-01 | 2014-12-17 | 临沂大学 | Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor |
| CN104480018A (en) * | 2014-12-11 | 2015-04-01 | 临沂大学 | Method for preparing and storing fruiting dunaliella algae paste |
| CN104488831A (en) * | 2015-01-15 | 2015-04-08 | 厦门大学 | Method for cultivating larval stage feed copepods for ocean fancy fishes |
-
2015
- 2015-04-13 CN CN201510173133.3A patent/CN104782576B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1405749A1 (en) * | 1986-08-04 | 1988-06-30 | Азовский научно-исследовательский институт рыбного хозяйства | Method of storing the eggs of rotizera at rest, brashiouus calyciflorous calycyflorus pallas |
| CN101731185A (en) * | 2009-12-23 | 2010-06-16 | 大连水产学院 | Large-scale cultivation method for marine floating harpacticoida |
| CN202588097U (en) * | 2012-04-09 | 2012-12-12 | 上海海洋大学 | Water flow buffering device |
| CN102948399A (en) * | 2012-04-13 | 2013-03-06 | 环境保护部南京环境科学研究所 | Daphnia magna breeding method for evaluating safety of transgenic rice |
| CN103834569A (en) * | 2014-03-03 | 2014-06-04 | 临沂大学 | Microalga concentration culture solution and application thereof |
| CN103966100A (en) * | 2014-05-26 | 2014-08-06 | 临沂大学 | Culture medium and culture method for culturing isochrysis galbana and heterogloea sp. by use of slaughtering plant wastewater |
| CN104212718A (en) * | 2014-09-01 | 2014-12-17 | 临沂大学 | Culture medium and culture method for culturing chlorella pyrenoidosa by sheep manure leach liquor |
| CN104480018A (en) * | 2014-12-11 | 2015-04-01 | 临沂大学 | Method for preparing and storing fruiting dunaliella algae paste |
| CN104488831A (en) * | 2015-01-15 | 2015-04-08 | 厦门大学 | Method for cultivating larval stage feed copepods for ocean fancy fishes |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104996365A (en) * | 2015-08-04 | 2015-10-28 | 厦门大学 | Screening and cultural method for initial feed bestiolina similis of ocean fancy fishes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104782576B (en) | 2017-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104170618B (en) | Zero-emission no pollution high-efficiency agriculture ecological recycle system | |
| CN104969886B (en) | A kind of fish and vegetable symbiotic breeding method | |
| CN205596919U (en) | Gather formula inner loop equipment of breeding fish | |
| CN104082248B (en) | The method of the simple and easy cultivation freshwater rotifer of a kind of small-scale | |
| CN204392947U (en) | A kind of fish and vegetable symbiotic water circulation cultivates planting unit | |
| CN104255632A (en) | Totally-closed all-transparent intellectualized organic prawn ecological breeding device | |
| CN103444634B (en) | Construction method for micro-ecosystem | |
| CN106342732A (en) | Loach fry culture method | |
| CN105706810A (en) | Novel method for culturing fish in rice field | |
| CN106035244A (en) | In situ enclosure-type large-scale farming method for cladocerans in freshwater lake | |
| CN104593262A (en) | Series cultivation and rapid collection method for marine microalgae | |
| CN104719669A (en) | Preparing and feeding method for chlamys farreri larva baits | |
| CN104054614A (en) | Ecological polyculture method of river cabs, freshwater shrimps and rhodeus sinensis | |
| CN105754903A (en) | Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale | |
| CN102057888A (en) | Artificial culturing method and device for large water flea | |
| CN103141422A (en) | Cultivation method for green crab seedlings in pond | |
| CN104521654B (en) | A kind of method that pond eutrophication is administered by rice cultivation | |
| CN103004662B (en) | Method for cultivating loach water flower seedlings by using fresh water benthic diatoms | |
| CN104719207A (en) | Method for cultivating branchionus plicatilis through waste plastic buckets | |
| CN110150200A (en) | Misgurnus auguillicaudatus seed method for breeding graded | |
| CN104480017A (en) | Efficient cultivating and harvesting method for nitzschia closterium | |
| CN205813024U (en) | A kind of pond Three-dimensional cultivating system | |
| CN107384832A (en) | A kind of method of inexpensive mass propgation Azotica | |
| CN106614171A (en) | High-efficiency river crab seedling growing integrated cultivation method | |
| CN104782576B (en) | A kind of cultural method with the wise water flea of Porcelain Jar culture flying horse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201217 Address after: 331300 Chengbei Industrial Park, Xingan County, Ji'an City, Jiangxi Province Patentee after: Jiangxi Shuangjia Technology Co.,Ltd. Address before: Room 1602, B1 # Building, Lijinxiu Jiangnan (West District), east of Quyun Road, north of Yinhe No. 2 Road, Suzhou City, Anhui Province Patentee before: Anhui Huiteng Intellectual Property Co.,Ltd. Effective date of registration: 20201217 Address after: Room 1602, B1 # Building, Lijinxiu Jiangnan (West District), east of Quyun Road, north of Yinhe No. 2 Road, Suzhou City, Anhui Province Patentee after: Anhui Huiteng Intellectual Property Co.,Ltd. Address before: 276000 Linyi University, middle section of Shuangling Road, Linyi City, Shandong Province Patentee before: LINYI University |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170929 Termination date: 20210413 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |