CN104782565A - Giant spiny frog inducing spawning method - Google Patents
Giant spiny frog inducing spawning method Download PDFInfo
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- CN104782565A CN104782565A CN201510172847.2A CN201510172847A CN104782565A CN 104782565 A CN104782565 A CN 104782565A CN 201510172847 A CN201510172847 A CN 201510172847A CN 104782565 A CN104782565 A CN 104782565A
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Abstract
The invention provides a giant spiny frog inducing spawning method which comprises the steps that in April, when air temperature is above 20 DEG C, giant spiny frog parents with the female-male ratio of 1 : 1 are placed in a reproduction frog pool for hybridization and spawning, the released density is 1-2 pairs per square meter, the reproduction frog pool is arranged in a reproduction frog chamber, indoor illumination intensity is controlled to be 2000-5000 lux, natural illumination time is kept, pool water temperature is kept at 19-20 DEG C, and dissolved oxygen is at least 5 mg/L. According to the method, the problem that in the prior art, giant spiny frog parent reproduction and spawning cost is high, time and labor are wasted, and frog parents are damaged is solved, the problem that spawning time is interrupted, and large-batch offspring seeds with the same specification cannot be obtained at the same time is solved, the giant spiny frog inducing spawning method can guarantee giant spiny frog parent reproduction spawning size specification orderliness and enable cost to be low, and frog parents are not damaged.
Description
Technical field
The invention belongs to aquaculture field, be specifically related to a kind of Ranaspinosa David induced spawning method.
Background technology
Ranaspinosa David has another name called chukar, the sour jujube frog, stone squama, the stone frog, stone clam etc., and belong to amphibian animal ranid, being mainly distributed in the provinces such as Zhejiang, Anhui, Jiangxi, Hubei, Hunan, Fujian, Guangdong, Guangxi and Hainan Island, is the distinctive large-scale wild frog of China.Ranaspinosa David mild in medicine property, taste is sweet, enters the heart, liver, stomach three warp, has nourishing Yin and falling fire, heart clearing, lung moistening, strong liver stomach kidney, nourishing and fit keeping function, qi-restoratives damage and the effect such as relieving heat toxin, also have certain auxiliary curative effect for treatment infantile malnutrition due to digestive disturbances or intestinalparasites, after being ill weakness, restlessness and thirst etc.Ranaspinosa David not only has very high medical value, also there is very high edibility, the multiple nutritional components such as protein, glucose, amino acid, iron, phosphorus and vitamin are rich in its frog meat, and fat content is very low, edibility is far away higher than bullfrog, be always the first-class delicacies in feast, because its meat is fine and smooth, taste is sweet and refreshing and be rich in abundant nutrition and be referred to as " kings of hundred frogs " by gourmet, be usually classified as mountain delicacy famous dish by restaurant and hotel.Because Ranaspinosa David has very high medical value and edibility, make it become the up-and-coming youngster of China's frog culture industry gradually, become the new focus of increasing peasant income, develop very fast, there are boundless market prospects.
At present; the numerous cultivation of domestic Ranaspinosa David is flourish; breeding for Ranaspinosa David is laid eggs and has been done large quantifier elimination, is all be that Ranaspinosa David creates suitable spawning place mostly about the breeding of Ranaspinosa David in research data, allows its natural spawning; although but such time and labour saving; but it cannot obtain the synchronous fertilized egg of large batch of growth, cannot obtain the Ranaspinosa David seed of neat specification in batches, the active fault of laying eggs of the close frog is continuous; whole breeding period is delayed very long, is unfavorable for the problem of large-scale production.Also the breeding adopting artificial induced spawning technology to impel Ranaspinosa David is had in prior art, although this technology can overcome the problems referred to above, obtain the neat fertilized egg in enormous quantities of size specification and Ranaspinosa David seed, but its cost is relatively high, time-consumingly to take a lot of work, and hormone can be used to the close frog in the process of artificial induced spawning, cause the damage healthy to the close frog.
Summary of the invention
For this reason, it is high that technical problem to be solved by this invention to be in prior art that the Ranaspinosa David parent frog breeds the cost compare of laying eggs, time-consumingly to take a lot of work, and the close frog is had to the problem of damage, and then a kind of Ranaspinosa David seed that can guarantee to obtain neat specification in enormous quantities is provided, cost compare can be made again low and to the close frog undamaged Ranaspinosa David induced spawning method.
For solving the problems of the technologies described above, a kind of Ranaspinosa David induced spawning method of the present invention, be included in April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density is every square metre of 1-2 couple, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 2000-5000lux, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is the step of more than 5mg/L.
Described Ranaspinosa David induced spawning method, described indoor illumination intensity is 3000lux.
Described Ranaspinosa David induced spawning method, described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 2-5m
3/ hour, keep perennial draingage, with natural imitation circle spring sound and moist natural environment.
Described Ranaspinosa David induced spawning method, the described kind of outdoor roof of the frog is provided with water injector, timed spays every day roof 2-3 hour, plays the effect imitating the patter of rain, promotes Ranaspinosa David Maturation and spawning.
Described Ranaspinosa David induced spawning method, described kind of frog pond is cement pit, and area is 4-6m
2, pond height is 1-1.5m, and tilt to delivery port at the bottom of pond, gradient is 10-15 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 2-5m
3/ hour.
Preferably, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour.
Described Ranaspinosa David induced spawning method, is placed with some cobbles in described kind of frog pond, and described cobble having kept flat area is 0.4-0.6m
2plank as the terrestrial of described Ranaspinosa David and bait table.
Described Ranaspinosa David induced spawning method, also comprises the described kind of frog and carry out the step of reinforced cultivating before starting to breed, and to feed the described kind of frog with earthworm, yellow mealworm animal living bait, scale of feeding is the 4-5% of described close frog body weight.
Described Ranaspinosa David induced spawning method, the step that described kind of frog carries out reinforced cultivating before starting to breed also comprises the step of regularly feed with the method for manually filling with food loach and/or pork liver.
Technique scheme of the present invention has the following advantages compared to existing technology,
(1) Ranaspinosa David induced spawning method of the present invention, lay eggs by the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed, breeding density is every square metre of 1-2 couple, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 2000-5000lux, timing every day light application time is 10-12 hour, and keep pond water temperature 19-20 DEG C, dissolved oxygen is the step of more than 5mg/L, the intensity of illumination of being planted frog pond by control is 2000-5000lux, be conducive to promoting that the frog experiences seasonal variation, promote that the synchronous ripe breeding of Ranaspinosa David parent frog sexual gland is laid eggs, suitable and in scale Seedling production, can produce in batches, easy acquisition is with batch same specification seed, owing to not using hormone, do not damage the close frog, nuisanceless, simple to operate, human cost is low,
(2) Ranaspinosa David induced spawning method of the present invention, by kind of the water inlet in frog pond being located at the top in pond, water inlet is provided with tap and drips in pond with certain flow rate, Ranaspinosa David growth spring streams, simulation mountain area environment, to simulate mountain spring environmental induction, lay eggs for Ranaspinosa David and create adapt circumstance condition;
(3) Ranaspinosa David induced spawning method of the present invention, by being provided with water injector at the outer top of kind of frog room, timing every day, to roof water spray 2-3 hour, is simulated the patter of rain that gurgles, is laid eggs to stimulate Ranaspinosa David gonadal maturation;
(4) Ranaspinosa David induced spawning method of the present invention, is set to cement pit by kind of a frog pond, and area is 4-6m
2, pond height is 1.2m, tilts at the bottom of pond to delivery port, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and the flow velocity controlling water is 2-5m
3/ hour, increased water flow amount, imitation current in rainy season are rapid, lay eggs to stimulate Ranaspinosa David gonadal maturation;
(5) Ranaspinosa David induced spawning method of the present invention, described bait comprises loach and pork liver, adopts regularly artificial filling food method, the kind frog sexual maturity that gonad development can be promoted bad, is conducive to Ranaspinosa David parent frog sexual gland and synchronously reaches maturity;
(6) to have cost low for Ranaspinosa David induced spawning method of the present invention, and do not need specific device, existing breeding facility is transformed a little and can be used.
Embodiment
Embodiment 1
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 2 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 2000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, general 1 yellow mealworm bait of throwing something and feeding every day afternoon 17 time, daily ration, feeding quantity is 5% of described froglet body weight.
Embodiment 2
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 5000lux, the controlled light time is that the morning 6 is up to afternoon 18 time,, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L.Conventionally feed Ranaspinosa David, 1 angleworm bait of generally throwing something and feeding every day afternoon 18 time, daily ration, feeding quantity is 4% of described froglet body weight.
Embodiment 3
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keep pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L.Conventionally feed the mixed feed of Ranaspinosa David, generally throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight.
Embodiment 4
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 2m
3/ hour, described kind of frog pond is cement pit, and area is 6m
2, pond height is 1m, and tilt to delivery port at the bottom of pond, gradient is 15 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and the flow velocity controlling water is 5m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.4m
2plank as the terrestrial of described Ranaspinosa David and bait table.
Embodiment 5
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 5m
3/ hour, described kind of frog pond is cement pit, and area is 4m
2, pond height is 1.5m, and tilt to delivery port at the bottom of pond, gradient is 10 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 5m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.6m
2plank as the terrestrial of described Ranaspinosa David and bait table.
Embodiment 6
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 3m
3/ hour, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.5m
2plank as the terrestrial of described Ranaspinosa David and bait table.
Embodiment 7
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 2 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog carries out reinforced cultivating before starting breeding, and to feed the described kind of frog with angleworm bait, scale of feeding is 5% of described close frog body weight.
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 3m
3/ hour, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.5m
2plank as the terrestrial of described Ranaspinosa David and bait table.
Embodiment 8
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog carries out reinforced cultivating before starting breeding, and to feed the described kind of frog with yellow mealworm bait, scale of feeding is 4% of described close frog body weight.
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 3m
3/ hour, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.5m
2plank as the terrestrial of described Ranaspinosa David and bait table.The described kind of outdoor roof portion of the frog is provided with water injector, 2 hours, timed spays every day roof.
Embodiment 9
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 2 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog carries out reinforced cultivating before starting breeding, and to feed the described kind of frog with the mixed feed of earthworm and yellow mealworm, scale of feeding is 5% of described close frog body weight.
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 3m
3/ hour, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.5m
2plank as the terrestrial of described Ranaspinosa David and bait table.The described kind of outdoor roof portion of the frog is provided with water injector, 3 hours, timed spays every day roof.
Embodiment 10
Ranaspinosa David induced spawning method described in the present embodiment, specifically comprises the steps:
April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density be every square metre 1 right, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 3000lux, the controlled light time is that the morning 6 is up to afternoon 18 time, keep natural lighting duration, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is more than 5mg/L, and Ranaspinosa David of conventionally feeding, the mixed feed of general throw something and feed every day afternoon 17 time 1 yellow mealworm and earthworm, daily ration, feeding quantity is 5% of described froglet body weight;
Described kind of frog carries out reinforced cultivating before starting breeding, and to feed the described kind of frog with the mixed feed of earthworm and yellow mealworm, scale of feeding is 5% of described close frog body weight; Also comprise in above-mentioned steps and regularly to feed loach and/or pork liver step with the method for manually filling with food.
Described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 3m
3/ hour, described kind of frog pond is cement pit, and area is 5m
2, pond height is 1.2m, tilts at the bottom of pond to delivery port, and gradient is 12 ° of water controlled in pond by described water inlet and described delivery port is perennial draingage, and flow velocity is 3m
3/ hour, be placed with some cobbles in described kind of frog pond, described cobble having kept flat area is 0.5m
2plank as the terrestrial of described Ranaspinosa David and bait table.The described kind of outdoor roof portion of the frog is provided with water injector, 2.5 hours, timed spays every day roof.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (9)
1. a Ranaspinosa David induced spawning method, it is characterized in that, be included in April, when temperature is more than 20 DEG C, the Ranaspinosa David parent frog taking female-male proportion as 1:1 is positioned in kind of frog pond to breed and lays eggs, breeding density is every square metre of 1-2 couple, kind of a frog indoor are located in described kind of frog pond, and to control indoor illumination intensity be 2000-5000lux, and the controlled light time is that the morning 6 is up to afternoon 18 time, and keeping pond water temperature 19-20 DEG C, dissolved oxygen is the step of more than 5mg/L.
2. Ranaspinosa David induced spawning method according to claim 1, is characterized in that, described indoor illumination intensity is 3000lux.
3. Ranaspinosa David induced spawning method according to claim 1 and 2, is characterized in that, described kind of frog pond water inlet is located at the top in described kind of frog pond, and described water inlet is provided with tap and drips in pond, and flow velocity is 2-5m
3/ hour.
4. the Ranaspinosa David induced spawning method according to any one of claim 1-3, is characterized in that, the outdoor roof portion of described kind of frog is provided with water injector, roof 2-3 hour described in every day timed spays.
5. the Ranaspinosa David induced spawning method according to any one of claim 1-4, it is characterized in that, described kind of frog pond is cement pit, area is 4-6m
2, pond height is 1-1.5m, and tilt to delivery port at the bottom of pond, gradient is 10-15 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and the flow velocity controlling water is 2-5m
3/ hour.
6. the Ranaspinosa David induced spawning method according to any one of claim 1-5, it is characterized in that, described kind of frog pond is cement pit, area is 5m
2, pond height is 1.2m, and tilt to delivery port at the bottom of pond, gradient is 12 °, and the water controlled in pond by described water inlet and described delivery port is perennial draingage, and the flow velocity controlling water is 3m
3/ hour.
7. the Ranaspinosa David induced spawning method according to any one of claim 1-6, is characterized in that, is placed with some cobbles in described kind of frog pond, and described cobble having kept flat area is 0.4-0.6m
2plank as the terrestrial of described Ranaspinosa David and bait table.
8. the Ranaspinosa David induced spawning method according to any one of claim 1-7, it is characterized in that, also comprise the described kind of frog and carry out the step of reinforced cultivating before starting to breed, to feed the described kind of frog with earthworm and/or yellow mealworm animal living bait, scale of feeding is the 4-5% of described close frog body weight.
9. Ranaspinosa David induced spawning method according to claim 8, is characterized in that, the step that described kind of frog carries out reinforced cultivating before starting to breed also comprises the step of regularly feed with the method for manually filling with food loach and/or pork liver.
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| CN106359298A (en) * | 2016-10-19 | 2017-02-01 | 浙江海洋大学 | Giant spiny frog culture method |
| CN109349232A (en) * | 2018-11-30 | 2019-02-19 | 三明市三元区众源棘胸蛙养殖专业合作社 | A kind of Ranaspinosa David combined biological cultivating system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106305590A (en) * | 2016-08-12 | 2017-01-11 | 英德市盈信农业有限公司 | Ecological collection method of giant spiny frog eggs |
| CN106359298A (en) * | 2016-10-19 | 2017-02-01 | 浙江海洋大学 | Giant spiny frog culture method |
| CN109349232A (en) * | 2018-11-30 | 2019-02-19 | 三明市三元区众源棘胸蛙养殖专业合作社 | A kind of Ranaspinosa David combined biological cultivating system |
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