CN104755625B - 使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法 - Google Patents

使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法 Download PDF

Info

Publication number
CN104755625B
CN104755625B CN201380055160.XA CN201380055160A CN104755625B CN 104755625 B CN104755625 B CN 104755625B CN 201380055160 A CN201380055160 A CN 201380055160A CN 104755625 B CN104755625 B CN 104755625B
Authority
CN
China
Prior art keywords
methionine
homoserine
phospho
amino acid
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380055160.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN104755625A (zh
Inventor
缪里·梅尔卡姆
多米尼克·路易斯
卡琳·加拉尔东
多米尼克·托马斯
菲力浦·马利尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adisseo France SAS
Original Assignee
Adisseo France SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Adisseo France SAS filed Critical Adisseo France SAS
Publication of CN104755625A publication Critical patent/CN104755625A/zh
Application granted granted Critical
Publication of CN104755625B publication Critical patent/CN104755625B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P11/00—Preparation of sulfur-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/38—Nucleosides
    • C12P19/40—Nucleosides having a condensed ring system containing a six-membered ring having two nitrogen atoms in the same ring, e.g. purine nucleosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01048—Cystathionine gamma-synthase (2.5.1.48)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
CN201380055160.XA 2012-10-26 2013-10-25 使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法 Active CN104755625B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12190150.8 2012-10-26
EP12190150 2012-10-26
PCT/EP2013/072380 WO2014064244A2 (en) 2012-10-26 2013-10-25 Means and methods for the enzymatic production of l-methionine from o-phospho-l-homoserine and methanethiol

Publications (2)

Publication Number Publication Date
CN104755625A CN104755625A (zh) 2015-07-01
CN104755625B true CN104755625B (zh) 2020-02-07

Family

ID=47071182

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380055160.XA Active CN104755625B (zh) 2012-10-26 2013-10-25 使用突变的胱硫醇γ-合酶从O-磷酸-L-高丝氨酸和甲硫醇制备L-甲硫氨酸的方法

Country Status (10)

Country Link
US (1) US9803225B2 (enExample)
EP (1) EP2912185A2 (enExample)
JP (1) JP6438402B2 (enExample)
KR (1) KR102126318B1 (enExample)
CN (1) CN104755625B (enExample)
BR (1) BR112015007212B1 (enExample)
IN (1) IN2015DN02439A (enExample)
MY (1) MY176164A (enExample)
RU (1) RU2650859C2 (enExample)
WO (1) WO2014064244A2 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10382927B2 (en) * 2015-08-20 2019-08-13 Samsung Electronics Co., Ltd. Method of text input for wearable devices
EP3330380A1 (en) * 2016-12-05 2018-06-06 Evonik Degussa GmbH Process for producing l-methionine from methional
US11162122B2 (en) 2017-07-11 2021-11-02 Adisseo France S.A.S. Methionine-producing yeast
KR102025867B1 (ko) * 2017-07-13 2019-09-27 씨제이제일제당 주식회사 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법
EP3875590A4 (en) * 2018-10-30 2023-02-15 Green Earth Institute Co., Ltd. PROCESS FOR THE PRODUCTION OF AN ORGANIC COMPOUND AND CORYNEFORM BACTERIUM
US20220315965A1 (en) * 2019-06-25 2022-10-06 Zymergen Inc. Engineered biosynthetic pathways for production of cystathionine by fermentation
KR102744891B1 (ko) 2022-03-24 2024-12-19 씨제이제일제당 주식회사 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법
JP2026508194A (ja) * 2023-03-01 2026-03-10 フーナン リアー バイオテック カンパニー リミテッド Ｌ－メチオニンをｌ－ホモセリンから直接調製するプロセス
CN117165549B (zh) * 2023-08-30 2026-08-11 天津科技大学 一种胱硫醚-γ-合酶突变体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017112A1 (en) * 1992-02-20 1993-09-02 Genencor International, Inc. Biosynthesis of methionine using a reduced source of sulfur
WO2011031285A1 (en) * 2009-09-08 2011-03-17 Shai Ufaz Improving essential amino acid content in algae and cyanobacteria for animal feed

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1846547A2 (en) * 2005-02-07 2007-10-24 Metabolic Explorer Microorganisms comprising enzymes expressed with low gamma-elimination activity
KR20080036608A (ko) * 2005-07-18 2008-04-28 바스프 에스이 메티오닌 생산 재조합 미생물

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017112A1 (en) * 1992-02-20 1993-09-02 Genencor International, Inc. Biosynthesis of methionine using a reduced source of sulfur
WO2011031285A1 (en) * 2009-09-08 2011-03-17 Shai Ufaz Improving essential amino acid content in algae and cyanobacteria for animal feed

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions;Vartanian J P et al;《Nucleic Acids Research》;19961231;2627-2631 *
Purification and properties of cystathionine gamma-synthase from wheat(Triticum aestivum L);Kreft B D et al;《Plant Physiology》;19941231;1215-1220 *
代谢途径分析在S－腺苷甲硫氨酸生产中的应用;郑荣勤;《广西轻工业》;20090131;8－9 *

Also Published As

Publication number Publication date
KR20150076159A (ko) 2015-07-06
WO2014064244A3 (en) 2014-08-14
US9803225B2 (en) 2017-10-31
BR112015007212A2 (pt) 2018-01-30
JP2015533280A (ja) 2015-11-24
JP6438402B2 (ja) 2018-12-12
US20150275247A1 (en) 2015-10-01
RU2650859C2 (ru) 2018-04-17
IN2015DN02439A (enExample) 2015-09-04
KR102126318B1 (ko) 2020-06-25
MY176164A (en) 2020-07-24
CN104755625A (zh) 2015-07-01
WO2014064244A2 (en) 2014-05-01
RU2015119400A (ru) 2016-12-20
BR112015007212B1 (pt) 2022-07-05
EP2912185A2 (en) 2015-09-02

Similar Documents

Publication Publication Date Title
JP6438402B2 (ja) Ｏ−ホスホ−ｌ−ホモセリンおよびメタンチオールからｌ−メチオニンを酵素的に産生する手段および方法
JP5497628B2 (ja) 組成物およびメチオニン生産方法
EP2314710B1 (en) Method for the production of methionine by culturing a microorganism modified to enhance production of cysteine
MX2011001962A (es) Produccion de metionina sin n-acil-metionina.
JP2008529485A (ja) 低いγ脱離活性を有する発現酵素を含む微生物
US20050255568A1 (en) Methods and compositions for amino acid production
JP2010530223A (ja) 調節解除されたビタミンｂ１２系を有する微生物
CN109790106A (zh) N-乙酰基高丝氨酸
US9234223B2 (en) Method for producing L-cysteine
US20140342399A1 (en) Microorganism and method for overproduction of gamma-glutamylcysteine and derivatives of this dipeptide by fermentation
WO2021050371A1 (en) Biotin synthases for efficient production of biotin
TWI884715B (zh) 從l-高絲胺酸直接製備l-甲硫胺酸的方法
JP2017143756A (ja) Ｌ−システインの製造法
CN103710319B (zh) 组合物及甲硫氨酸的生产方法
CN102703398B (zh) 组合物及甲硫氨酸的生产方法
WO2015067331A1 (en) S-adenosylmethionine (sam) synthase variants for the synthesis of artificial cofactors
HK1102828A (en) Recombinant enzyme with altered feedback sensitivity

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant