CN104745533A - 产生非节段负链rna 病毒的细胞和方法 - Google Patents
产生非节段负链rna 病毒的细胞和方法 Download PDFInfo
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109913423A (zh) * | 2019-03-27 | 2019-06-21 | 扬州大学 | 一种稳定表达猪德尔塔冠状病毒N蛋白的重组Vero细胞系及应用 |
| CN111218474A (zh) * | 2018-11-23 | 2020-06-02 | 成都生物制品研究所有限责任公司 | 一种副粘病毒的拯救系统和副粘病毒拯救方法 |
| CN120648655A (zh) * | 2025-08-21 | 2025-09-16 | 内蒙古自治区农牧业科学院 | 一株t7-293t细胞及其在牛副流感病毒3型感染性克隆拯救病毒中的应用 |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DK1375512T3 (da) | 2002-06-20 | 2009-10-26 | Pasteur Institut | Infektiös cDNA af en godkendt vaccinestamme af mæslingevirus. Anvendelse til immunogene sammensætninger |
| EP1375670B1 (en) | 2002-06-20 | 2013-06-12 | Institut Pasteur | Recombinant measles viruses expressing epitopes of antigens of RNA viruses and use of the recombinant viruses for the preparation of vaccine compositions |
| CA2432738A1 (en) | 2003-02-26 | 2004-08-26 | Philippe Despres | New dengue and west nile viruses proteins and genes coding the foregoing, and their use in vaccinal, therapeutic and diagnostic applications |
| WO2012038832A2 (en) * | 2010-09-24 | 2012-03-29 | Institut Pasteur | Generation of replicating chimeric measles virus - retrovirus particles |
| CN103906843B (zh) * | 2011-06-08 | 2016-12-07 | 维什瓦斯·乔希 | 双质粒哺乳动物表达系统 |
| FR2994697B1 (fr) * | 2012-08-24 | 2016-05-13 | Centre De Coop Int En Rech Agronomique Pour Le Dev (Cirad) | Souche vaccinale marquee du virus de la peste des petits ruminants, et son procede d'obtention |
| EP2712871A1 (en) | 2012-09-27 | 2014-04-02 | Institut Pasteur | Recombinant Measles virus expressing Chikungunya virus polypeptides and their applications |
| EP2759301A1 (en) | 2013-01-24 | 2014-07-30 | Institut Pasteur | Use of a genetically modified infectious measles virus with enhanced pro-apoptotic properties (MV-DeltaC virus) |
| US20140255447A1 (en) * | 2013-03-05 | 2014-09-11 | Biomune Company | Production of avian embryo cells |
| WO2015092710A1 (en) | 2013-12-19 | 2015-06-25 | Glaxosmithkline Biologicals, S.A. | Contralateral co-administration of vaccines |
| EP3242939A4 (en) | 2015-01-05 | 2018-10-17 | Seagull BioSolutions Pvt. Ltd. | Dna molecules producing custom designed replicating and non-replicating negative stranded rna viruses and uses there of |
| EP3184119A1 (en) | 2015-12-23 | 2017-06-28 | Themis Bioscience GmbH | Chromatography based purification strategies for measles scaffold based viruses |
| EP3255056A1 (en) * | 2016-06-06 | 2017-12-13 | Commissariat À L'Énergie Atomique Et Aux Énergies Alternatives | Method for producing self-assemblying paramyxoviral nucleocapsid-like particles and their uses |
| US11351246B2 (en) | 2017-05-09 | 2022-06-07 | Invectys SAS | Recombinant measles vaccine expressing hTERT |
| EP3412307A1 (en) | 2017-06-07 | 2018-12-12 | Institut Pasteur | Recombinant measles virus expressing zika virus proteins and their applications |
| CN107557340A (zh) * | 2017-09-26 | 2018-01-09 | 南京普菲科医药科技有限公司 | 一种可诱导性拯救弹状病毒的细胞系及其构建方法与应用 |
| SG11202004234SA (en) | 2017-11-09 | 2020-06-29 | Pasteur Institut | A zika virus chimeric polyepitope comprising non-structural proteins and its use in an immunogenic composition |
| AU2018392826B2 (en) | 2017-12-21 | 2025-10-09 | Centre National De La Recherche Scientifique | Lassa vaccine |
| EP3820884B1 (en) * | 2018-07-13 | 2025-09-03 | Valneva SE | Method for rescuing and producing a virus in avian cells |
| CN109355301A (zh) * | 2018-08-22 | 2019-02-19 | 深圳大学 | 一种石斑鱼重组NNVcp的密码子优化的核酸序列、融合表达载体、制备方法及其本身 |
| EP3673917A1 (en) | 2018-12-28 | 2020-07-01 | Themis Bioscience GmbH | Norovirus vaccines |
| CA3166811A1 (en) | 2020-02-13 | 2021-08-19 | Nicolas Robert Xavier Escriou | Measles-vectored covid-19 immunogenic compositions and vaccines |
| EP3865180A1 (en) | 2020-02-13 | 2021-08-18 | Institut Pasteur | Live recombinant measles virus expressing coronavirus antigens - its use in eliciting immunity against coronaviruses |
| EP4142786A4 (en) * | 2020-04-27 | 2024-06-19 | Ohio State Innovation Foundation | LIVE ATTENUATED MEASLES VIRUS VACCINE AGAINST SARS-COV-2 |
| EP3936517A1 (en) * | 2020-07-08 | 2022-01-12 | Institut Pasteur | An improved measles virus vaccine vector based on multiple tandem additional transcription units |
| CA3204201A1 (en) | 2021-01-13 | 2022-07-21 | Thierry Heidmann | Measles-hiv or measles-htlv vaccine |
| US20250304921A1 (en) * | 2022-05-27 | 2025-10-02 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Reverse genetics system for feline morbillivirus |
| EP4501946A1 (en) | 2023-07-31 | 2025-02-05 | LoopLab Bio GmbH | Self-amplifying biologics for targeted therapy |
| WO2026013289A1 (en) | 2024-07-12 | 2026-01-15 | Institut Pasteur | Measles-mumps-rubella (mmr) vaccines |
Family Cites Families (9)
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| ATE181112T1 (de) * | 1995-08-09 | 1999-06-15 | Schweiz Serum & Impfinst | Dem genom von minussträngigen rna-viren entsprechende cdna und verfahren zur herstellung von infektiösen minussträngigen rna-viren |
| HK1050210B (en) | 1999-10-12 | 2007-08-24 | Institut Pasteur | Lentiviral triplex dna, and vectors and recombinant cells containing lentiviral triplex dna |
| JP2004503233A (ja) | 2000-06-16 | 2004-02-05 | インスティティ・パスツール | デング熱ウイルスエンベロープ糖タンパク質のプロ−アポトーシスフラグメント |
| EP1375670B1 (en) | 2002-06-20 | 2013-06-12 | Institut Pasteur | Recombinant measles viruses expressing epitopes of antigens of RNA viruses and use of the recombinant viruses for the preparation of vaccine compositions |
| DE10234579A1 (de) * | 2002-07-30 | 2004-02-12 | Zf Friedrichshafen Ag | Getriebe |
| EP1633860A2 (en) * | 2003-06-09 | 2006-03-15 | Wyeth | IMPROVED METHOD FOR THE RECOVERY OF NON-SEGMENTED, NEGATIVE-STRANDED RNA VIRUSES FROM cDNA |
| CA2549188A1 (fr) | 2003-12-02 | 2005-06-23 | Universite Paris 7 | Utilisation des proteines et des peptides codes par le genome d'une nouvelle souche de coronavirus associe au sras |
| FR2870126B1 (fr) | 2004-05-17 | 2009-07-17 | Pasteur Institut | Vecteur lentiviral recombinant d'expression d'une proteine de flaviviridae et ses applications comme vaccin |
| JP2008535868A (ja) * | 2005-04-14 | 2008-09-04 | ザ ユニバーシティー オブ クイーンズランド | 免疫調節組成物およびそのための使用 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111218474A (zh) * | 2018-11-23 | 2020-06-02 | 成都生物制品研究所有限责任公司 | 一种副粘病毒的拯救系统和副粘病毒拯救方法 |
| CN109913423A (zh) * | 2019-03-27 | 2019-06-21 | 扬州大学 | 一种稳定表达猪德尔塔冠状病毒N蛋白的重组Vero细胞系及应用 |
| CN120648655A (zh) * | 2025-08-21 | 2025-09-16 | 内蒙古自治区农牧业科学院 | 一株t7-293t细胞及其在牛副流感病毒3型感染性克隆拯救病毒中的应用 |
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| KR101469007B1 (ko) | 2014-12-05 |
| US9499799B2 (en) | 2016-11-22 |
| CN101631798A (zh) | 2010-01-20 |
| EP2091962A2 (en) | 2009-08-26 |
| CA2673262C (en) | 2019-03-05 |
| CA2673262A1 (en) | 2008-07-03 |
| MX2009006755A (es) | 2009-07-31 |
| DK2091962T3 (en) | 2018-10-08 |
| US20140051148A1 (en) | 2014-02-20 |
| WO2008078198A2 (en) | 2008-07-03 |
| US8586364B2 (en) | 2013-11-19 |
| EP2091962B1 (en) | 2018-07-04 |
| ES2423518T3 (es) | 2013-09-20 |
| WO2008078198A3 (en) | 2008-10-30 |
| BRPI0719469B1 (pt) | 2019-07-02 |
| ES2686168T3 (es) | 2018-10-16 |
| EP1939214B1 (en) | 2013-07-10 |
| JP2010512779A (ja) | 2010-04-30 |
| JP2015015955A (ja) | 2015-01-29 |
| EP1939214A1 (en) | 2008-07-02 |
| DK1939214T3 (da) | 2013-10-14 |
| JP5902371B2 (ja) | 2016-04-13 |
| BRPI0719469C1 (pt) | 2021-05-25 |
| KR20090103922A (ko) | 2009-10-01 |
| US20100144040A1 (en) | 2010-06-10 |
| BRPI0719469A2 (pt) | 2014-02-11 |
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