CN104692972A - Special bioactive organic fertilizer for cotton and application of fertilizer - Google Patents

Special bioactive organic fertilizer for cotton and application of fertilizer Download PDF

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Publication number
CN104692972A
CN104692972A CN201510094124.5A CN201510094124A CN104692972A CN 104692972 A CN104692972 A CN 104692972A CN 201510094124 A CN201510094124 A CN 201510094124A CN 104692972 A CN104692972 A CN 104692972A
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parts
cotton
organic fertilizer
fertilizer
biological activity
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CN104692972B (en
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胡新军
陈泽鹏
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Sun Yat Sen University
China National Tobacco Corp Guangdong Branch
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Sun Yat Sen University
China National Tobacco Corp Guangdong Branch
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a special bioactive organic fertilizer for cotton and an application of the fertilizer. The special bioactive organic fertilizer for cotton is prepared from an organic fertilizer prepared by treating inapplicable fresh tobacco leaves by using sarcophaga peregrina larvae, microbial bacterial powder and aninorganic salt fertilizer, wherein the microbial bacterial powder is mixed bacterial powder of nitrogen-fixing bacteria, bacillus subtilis and enterobacter cloacae. The bioactive organic fertilizer can meet the nutritional requirement rule of the cotton and has the effects of obviously accelerating growth, development and maturing rate of the cotton, greatly reducing the disease rate and greatly increasing the yield; meanwhile, the fertilizer is low in cost, convenient to apply and long in fertilizer effect; the problem of treatment and reutilization of the inapplicable fresh tobacco leaves are also solved; the new utilization value is given to the inapplicable fresh tobacco leaves; the wastes are utilized; the great economic benefits are generated; the fertilizer has a good prospect of popularization and application.

Description

One grows cotton biological activity organic fertilizer special and application thereof
Technical field
The invention belongs to biological special fertilizer technical field.More specifically, relate to one to grow cotton biological activity organic fertilizer special and application thereof.
Background technology
Cotton ( gossypium spp), be the seed hair of Malvaceae cotton, originate in subtropics, there are China, the U.S., India etc. in the country that output is the highest.Cotton is one of topmost farm crop in the world, and output is large, production cost is low, makes cotton products less expensive.Cotton fibre can make the fabric of plurality of specifications, from merrily and lightheartedly transparent voile fabric to abundant canvas and thick velveteen, is suitable for making all kinds of clothes, UPHOLSTERY FABRIC and technical cloth.Cotton fabric is strong wear-resisting, can wash and at high temperature presses, and cotton makes dress comfortable due to moisture absorption and dehumidification fast; Cotton or a kind of important nectariferous plant.
In recent years, cotton demand side, grind advisory data analysis Future Ten year world economic growth situation according to intelligence and have an optimistic view of, global cotton consumption keeps steady growth.India's textile industry develops rapidly, drives the increase of cotton consumption, estimates that its cotton consumption increment will account for 70% of global cotton consumption increment.In addition, cotton supply respect, global sown areas of cotton in 2013 reduce and cause output of cotton to decline to a great extent, Future Ten year whole world output of cotton by 2013, lower level slowly increases, reached to 2022 and slightly exceed the yield level of base period (2010-2012 average).Whole world output of cotton increasess slowly, and one side is the global repository that the exception in order to digest 2010-2013 accumulation is high; On the other hand, along with the rising of labor cost and the reduction of the relative grain specified value effect of cotton, the magnetism of Cotton Production is reduced.China is as global maximum Cotton Production state, and output of cotton will continue to reduce.Therefore, improve the output of cotton planting and ensure the quality of cotton, the enthusiasm keeping cotton planting and the economic benefit obtained could be continued.
And simultaneously, the annual inapplicable fresh tobacco leaf produced not only takes up room, the harm of environment is also become increasingly conspicuous.The present inventor's early-stage Study shows, and utilizes the various insects such as Sarcophga fuscicauda larva can process inapplicable fresh tobacco leaf, and obtains fertilizer and bioprotein simultaneously, achieves good effect.The bioprotein obtained is widely used, but for the fertilizer obtained, still further do not utilize, because there are some factors being unfavorable for crop growth in the fertilizer utilizing tobacco leaf to produce on the one hand, containing the particular matter being much different from common stalk or ight soil class fertilizer, directly apply to arable farming DeGrain, even certain restraining effect is existed to growing of plant, at present also not about the correlative study of this problem, be difficult to be applied to arable farming for the moment.
Summary of the invention
The technical problem to be solved in the present invention overcomes the deficiency of existing fertilizer special for cotton and the Utilizing question of inapplicable fresh tobacco leaf, there is provided one to grow cotton biological activity organic fertilizer special, this fertilizer is that the fertilizer that obtained by inapplicable for the process of Sarcophga fuscicauda larva fresh tobacco leaf and cotton growth probiotics combine and prepare.
Another object of the present invention is to provide the preparation method of described cotton biological activity organic fertilizer special.
Another object of the present invention is to provide the application of described cotton biological activity organic fertilizer special in cotton is cultivated.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
One grows cotton biological activity organic fertilizer special, the fertilizer obtained by the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process and microbial germ powder, then is equipped with inorganic salt fertilizer and prepares; Described microbial germ powder be vinelandii, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.Various microorganism strains of the present invention is all the bacterial classification categories from the commercially available National agricultural portion that meets relevant regulations, from the horse's mouth, and safe and effective.
As a kind of scheme, described cotton biological activity organic fertilizer special is made up of the component of following weight part: biological organic fertilizer 35 ~ 45 parts, microbial germ powder 2.5 ~ 6.0 parts, calcium superphosphate 3 ~ 4 parts, potassium sulfate 7 ~ 15 parts, potassium primary phosphate 1.5 ~ 3.2 parts, 1 ~ 2 part, calcium sulfate, 0.2 ~ 1 part, zinc sulfate, ammonium molybdate 1.3 ~ 2.9 parts, 3.2 ~ 4.8 parts, iron(ic) chloride, clay 5 ~ 15 parts, borax 10 ~ 15 parts; Wherein, described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains; The vinelandii of described microbial germ powder to be weight ratio be 1 ~ 3:1:0.5, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
Preferably, each ingredients weight parts of described cotton biological activity organic fertilizer special is: biological organic fertilizer 40 ~ 45 parts, microbial germ powder 4 ~ 5.5 parts, calcium superphosphate 3 ~ 4 parts, potassium sulfate 10 ~ 15 parts, potassium primary phosphate 1.5 ~ 2 parts, 1.5 parts, calcium sulfate, 0.5 ~ 0.8 part, zinc sulfate, ammonium molybdate 2 ~ 2.5 parts, 3.5 ~ 4 parts, iron(ic) chloride, clay 8 ~ 10 parts, borax 10 ~ 12 parts.
Preferably, the vinelandii of described microbial germ powder to be weight ratio be 1.5:1:0.5, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
More preferably, each ingredients weight parts of described cotton biological activity organic fertilizer special is: biological organic fertilizer 40 parts, microbial germ powder 5 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts.
In addition, preferably, described vinelandii be azotobacter chroococcum ( azotobacter chroococum) or India Bai Yelin kirschner bacterium ( beijerinckia indica).
More preferably, described vinelandii be azotobacter chroococcum ( azotobacter chroococum).
The preparation process of above-mentioned cotton biological activity organic fertilizer special is as follows:
S1. the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process is utilized to obtain fertilizer;
S2. according to weight ratio, after calcium superphosphate, potassium sulfate, potassium primary phosphate, calcium sulfate, zinc sulfate, ammonium molybdate, iron(ic) chloride, clay, borax are carried out fragmentation, mix thoroughly;
S3., after the particle obtained by S2 again and biological organic fertilizer mix, below moisture to 5% is dried;
S4. the material after drying and microbial germ powder mix, and granulation, obtains cotton biological activity organic fertilizer special.
Wherein, the method for step S1 is:
S11. collect the fragment that inapplicable fresh tobacco leaf is chopped into particle diameter 2 ~ 3cm, add auxiliary material and stir, be mixed with culture material;
S12. culture material surface access Sarcophga fuscicauda newly hatched larvae described in S11;
S13. Sarcophga fuscicauda newly hatched larvae started bio-transformation culture material in accessing the same day;
Taking food S14. through Sarcophga fuscicauda larva 4 ~ 5 days, culture material changes biological organic fertilizer into; Sarcophga fuscicauda larva reaches aging state.
Described culture material is made up of each component of following mass parts: inapplicable fresh tobacco leaf 60 ~ 85%, wheat bran 0 ~ 25%, chaff powder 5%, sawdust 5%, fish meal 5%.
The cotton biological activity organic fertilizer special utilizing above-mentioned preparation method to prepare is also within protection scope of the present invention.
Main purpose of the present invention is to make full use of inapplicable fresh tobacco leaf resource, utilization of waste material on the one hand, also eliminates the environmental problem that inapplicable fresh tobacco leaf produces on the other hand, realizes turning waste into wealth generation economic benefit simultaneously.The early-stage Study of the present inventor obtains a kind of method utilizing the inapplicable fresh tobacco leafs of various biological treatment such as Sarcophga fuscicauda larva simultaneously to obtain fertilizer and bioprotein, achieves good effect.The bioprotein obtained is widely used, but for the fertilizer obtained, does not still further utilize.In addition, as stated in the Background Art, the cultivation of cotton can not utilize chemical fertilizer merely, and best mode is that organic fertilizer and chemical fertilizer combination are used.But, organic fertilizer mainly farm manure conventional at present, as barnyard manure, people's muck, compost, vegetation fertilizer etc., the feature that nutrient is complete although these organic fertilizer have, fertilizer efficiency slow, the effect phase is long, but only should be used as base manure and seed manure, therefore mainly still rely on chemical fertilizer at present.In this case, the present inventor just take into account the novel biological fertilizer that early-stage Study obtains: utilize the fertilizer that the inapplicable fresh tobacco leaf of the various insect process such as Sarcophga fuscicauda larva obtains.
And for how realizing effective utilization of these biological organic fertilizers, to the growing of cotton, setting percentage, performance beneficial effect, suppress to reduce sickness rate, invention has been large quantifier elimination and exploration, first various vinelandii and biological organic fertilizer is utilized to carry out mixture, in addition, again coordinate utilize some can Promoting plant growth, process subterranean pest-insect probiotic bacterium, as: Bacillus popilliae, subtilis, bacillus megaterium, bacillusmusilaginosiengineering, Bacillus licheniformis, carry out composite with biological organic fertilizer.
Result of study shows, the amplitude improved by above-mentioned fertilizer and the composite promoter action of growing of fertilizer to cotton of vinelandii is merely little, and vinelandii and above-mentioned probiotic bacterium are coordinated jointly fertilizer, effect can strengthen greatly, but we also find, due to the singularity of microorganism strains, otherness between different strains, Survival significant difference in the fertilizer that different strains obtains at different insects process fresh tobacco leaf, and the fertilizer that different insects process fresh tobacco leaf obtains is after different strains effect, wherein the content of material is larger, therefore, different probiotic bacteriums and the inapplicable fresh tobacco leaf of different insects process obtain the combination of fertilizer, the final production-increasing function difference highly significant to cotton, the kind even amount ratio of vinelandii and probiotic bacterium, to the contribution obvious difference of final fertilizer efficiency.And we find, the fertilizer that the inapplicable fresh tobacco leaf of different insects process obtains also has very large selectivity for crop.
As at the beginning, we consider and maximumly in tobacco leaf are unfavorable for that the factor of plant-growth is exactly nicotine, but we show early-stage Study result, the fertilizer utilizing the inapplicable fresh tobacco leaf of the insect process such as Sarcophga fuscicauda to obtain is not wherein containing nicotine, or content is very little, obvious impact should be unable to be caused on the growth of plant, therefore we first by vinelandii and subtilis ( bacillus subtilis) etc. the common fertilizer obtained with process tobacco leaf of probiotic bacterium carry out composite, wish that obtaining one can grow by Promoting plant growth, the significant biological active organic fertilizer of fertilizer, but result but makes us feel very unexpected, although fertilizer Middle nutrition element is very abundant, vinelandii also better can provide nitrogenous source for crop is continual, probiotic bacterium has the disease resistance that can improve plant, suppress the advantages such as harmful microbe growth, but cultivation experiments result shows, composite biological active organic fertilizer is not remarkable to the production-increasing function of crop, differ greatly with our expection.
Through a large amount of exploratory development, enterobacter cloacae is also incorporated in fertilizer formula by we, and cultivation experiments result makes us feel very unexpected.The fertilizer that inapplicable for the process of Sarcophga fuscicauda larva fresh tobacco leaf is obtained and vinelandii (especially azotobacter chroococcum ( azotobacter chroococum)), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) carry out mixture according to certain ratio, then carry out composite with suitable inorganic fertilizer, the nutritional needs rule of cotton can not only be met, to cotton grow and setting percentage has obvious promoter action, sickness rate can also be reduced significantly, obvious effect of increasing production.The inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtain fertilizer in not containing nicotine or content very little, why can the enterobacter cloacae of degrading nicotine add to come in, is the fertilizer efficiency difference produced so big? we analyze, may be in the fertilizer on the one hand utilizing tobacco leaf to produce except nicotine, also there is other the factor being unfavorable for crop growth, containing the particular matter being much different from common stalk or ight soil class fertilizer, and enterobacter cloacae also not only just can degrading nicotine, also effect may be had to the unfavorable factor of these the unknowns above-mentioned, on the other hand, the synergy of different microorganisms also can produce unexpected result, again on the one hand, dissimilar fertilizer is selectively to different crops, and this also needs a large amount of exploration and practices to sum up ability pattern out.Be at what kind of about real principle and mechanism, we are also cooperating with R&D institution and are studying.
In sum, the present invention obtain cotton biological activity organic fertilizer special optimum formula be: the fertilizer 40 parts that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains, microbial germ powder 5 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts; Wherein, described microbial germ powder to be weight ratio be 1.5:1:0.5 azotobacter chroococcum ( azotobacter chroococum), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.In formula, azotobacter chroococcum, subtilis and enterobacter cloacae can well coexist, wherein, vinelandii can endlessly for cotton provides nitrogenous source, subtilis can regulate Soil Micro-environment, be conducive to the release of the element such as indissoluble phosphorus in soil, and have restraining effect to harmful microorganism, enterobacter cloacae can act on the factor that some that bring because of tobacco leaf in fertilizer are unfavorable for plant-growth.The more important thing is, three's acting in conjunction, after the fertilizer of the inapplicable fresh tobacco leaf acquisition of Sarcophga fuscicauda larva process, make it can well be applied to the cultivation of cotton, and experimental result also shows, this effect is not merely the contribution of enterobacter cloacae, is the synergistic contribution of three.
The present invention has following significant beneficial effect:
The fertilizer that inapplicable for the process of Sarcophga fuscicauda larva fresh tobacco leaf obtains by the present invention and vinelandii (especially azotobacter chroococcum ( azotobacter chroococum)), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) carry out mixture according to certain ratio, carry out composite with suitable inorganic fertilizer again, obtain a kind of biological active organic fertilizer being exclusively used in cotton, this fertilizer can not only meet the nutritional needs rule of cotton, to cotton grow and setting percentage has obvious promoter action, sickness rate can also be reduced significantly, obvious effect of increasing production, and use conveniently, fertilizer efficiency is long, only need use once in planting season, impose once in fruiting period of blooming, popularizing application prospect is good.
Organic fertilizer wherein can activating soil, improved structure, increases soil aggregate, improves soil physical and chemical property, and be of value to root nodule bacterium activity, increase sources of nitrogen, culture fertility, and the important sources containing various nutrient elements, particularly micronutrient element, fertilizer efficiency is lasting.After fertilizer is manured into soil, various nutrient can be discharged continuously through microbial decomposition and absorb for cotton, constantly can also discharge carbonic acid gas, improve the photosynthesis environment of cotton.The humus that organic fertilizer is formed in soil has multiple stronger surge capability.The various organic acid that organic fertilizer decomposition produces and mineral acid can promote the conversion of insoluble phosphate in soil, improve P availability.Mineral manure nutrient content is high, fertilizer efficiency fast, organic fertilizer and mineral manure compounding application, and both learn from other's strong points to offset one's weaknesses, and emergency is helped mutually, give full play to the yield potential of fertilizer, can ensure the good quality and high output of cotton, improves fertilising benefit.
Vinelandii wherein can increase the nitrogen fixing capacity of cotton root greatly, and the supply continually for nitrogen has great importance.Probiotic bacterium subtilis and enterobacter cloacae have good promoter action for the resistance against diseases of cotton, growing etc. of root, and harmful microbe in soil can be suppressed to spread unchecked, enterobacter cloacae can also act on the adverse factor being unfavorable for growth and development of plants brought by tobacco leaf.Meanwhile, the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains also as the main energy sources material of microorganism, can well can promote activity and the propagation of microorganism, increase its vigor.
Utilize cotton biological activity organic fertilizer special of the present invention to cultivate cotton, not only growth is fast, and setting percentage is high, and shows the detected result of objectionable impurities, and the cotton oil yield of results is high, and importantly harmful substance contents is low, pollution-free, meets food safety.
In addition, utilize the fertilizer that the inapplicable fresh tobacco leaf of insect process obtains, vegetalitas stalk etc. can also be avoided to obtain fertilizer problem, the problem includes: seasonal dependence problem, also solve the process of inapplicable fresh tobacco leaf and heavy Utilizing question simultaneously, give the utility value that it is new, while utilization of waste material, create good economic benefit.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment does not limit in any form to the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
Unless stated otherwise, agents useful for same of the present invention and material are commercial.
Various microorganism strains of the present invention is all the bacterial classification categories from the commercially available National agricultural portion that meets relevant regulations, from the horse's mouth, and safe and effective.
embodiment 1 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 40 parts, microbial germ powder 5 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts according to following weight part.
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains.
The azotobacter chroococcum of described microbial germ powder to be weight ratio be 1.5:1:0.5 ( azotobacter chroococum), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2, preparation process is as follows:
S1. the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process is utilized to obtain fertilizer;
S11. collect the fragment that inapplicable fresh tobacco leaf is chopped into particle diameter 2 ~ 3cm, add auxiliary material and stir, be mixed with culture material;
S12. culture material surface access Sarcophga fuscicauda newly hatched larvae described in S11;
S13. Sarcophga fuscicauda newly hatched larvae started bio-transformation culture material in accessing the same day;
Taking food S14. through Sarcophga fuscicauda larva 4 ~ 5 days, culture material changes biological organic fertilizer into; Sarcophga fuscicauda larva reaches aging state;
S2. according to weight part ratio, after calcium superphosphate, potassium sulfate, potassium primary phosphate, calcium sulfate, zinc sulfate, ammonium molybdate, iron(ic) chloride, clay, borax are carried out fragmentation, mix thoroughly;
S3., after the particle obtained by S2 again and biological organic fertilizer mix, below moisture to 5% is dried;
S4. the material after drying and microbial germ powder mix, and granulation, obtains cotton biological activity organic fertilizer special.
embodiment 2 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 40 parts, microbial germ powder 5 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts according to following weight part.
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains.
The India Bai Yelin kirschner bacterium of described microbial germ powder to be weight ratio be 1.5:1:0.5 ( beijerinckia indica), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2, preparation method is with embodiment 1.
embodiment 3 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 45 parts, microbial germ powder 4 parts, calcium superphosphate 3 parts, potassium sulfate 7 parts, potassium primary phosphate 1.5 parts, 1.5 parts, calcium sulfate, 0.8 part, zinc sulfate, ammonium molybdate 2.5 parts, 3.5 parts, iron(ic) chloride, clay 8 parts, borax 12 parts according to following weight part;
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains;
The India Bai Yelin kirschner bacterium of described microbial germ powder to be weight ratio be 1.5:1:0.5 ( beijerinckia indica), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2, preparation method is with embodiment 1.
embodiment 4 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 40 parts, microbial germ powder 5.5 parts, calcium superphosphate 4 parts, potassium sulfate 15 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.6 part, zinc sulfate, ammonium molybdate 2.5 parts, 4 parts, iron(ic) chloride, clay 10 parts, borax 10 parts according to following weight part;
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains;
The azotobacter chroococcum of described microbial germ powder to be weight ratio be 1.5:1:0.5 ( azotobacter chroococum), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2, preparation method is with embodiment 1.
embodiment 5 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 45 parts, microbial germ powder 6.0 parts, calcium superphosphate 3.5 parts, potassium sulfate 10 parts according to following weight part, potassium primary phosphate 3.2 parts, 1 part, calcium sulfate, 1 part, zinc sulfate, ammonium molybdate 2.9 parts, 3.2 parts, iron(ic) chloride, clay 9 parts, borax 10 parts;
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains;
The azotobacter chroococcum of described microbial germ powder to be weight ratio be 1:1:0.5 ( azotobacter chroococum), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.2, preparation method is with embodiment 1.
embodiment 6 prepares cotton biological activity organic fertilizer special
1, raw material is prepared
Each component is prepared: biological organic fertilizer 35 parts, microbial germ powder 2.5 parts, calcium superphosphate 4 parts, potassium sulfate 13 parts according to following weight part, potassium primary phosphate 3.2 parts, 2 parts, calcium sulfate, 0.2 part, zinc sulfate, ammonium molybdate 1.3 parts, 4.8 parts, iron(ic) chloride, clay 5 parts, borax 15 parts;
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains;
The India Bai Yelin kirschner bacterium of described microbial germ powder to be weight ratio be 3:1:0.5 ( beijerinckia indica), subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2, preparation method is with embodiment 1.
embodiment 7 biological organic fertilizer kind is screened
1, biological active organic fertilizer is prepared according to the formula of embodiment 1 and preparation method, just for fertilizer formula, using the kind of biological organic fertilizer as variable, investigating following 5 kinds of biological organic fertilizers respectively fits in cotton biological activity organic fertilizer special of the present invention, on the impact that cotton seedling grows.
Described 5 kinds of biological organic fertilizers are respectively: Sarcophga fuscicauda larva, chrysomyia megacephala (big head golden fly) larva, black soldier flies larva, lucilia sericata larvae, housefly process the fertilizer of inapplicable fresh tobacco leaf acquisition respectively according to identical method (described in embodiment 1).
2, test method
(1) tested among a small circle by cotton seedling in experimental plot, Zengcheng and screen.By uniform segmentation, experimental plot, first use the above-mentioned all types of cotton biological activity organic fertilizer specials of equivalent respectively, often organize land used 0.5 mu.
(2) conventionally cultivated cotton, evenly spread fertilizer over the fields fertilizer before whole ground, plough soil, make fertilizer side-lower 10 ~ 12 centimeters under soil greatly, rate of fertilizer application is 40 kgs/acre.
Carry out the management of cotton conventional cultivation after seed germination, after germination 5 days time, screening is carried out to cotton seedling and pulls out, the seedling that reservation growing way etc. are consistent as far as possible, obviously inconsistent, excessive, too small seedling is pulled out, meanwhile, ensures that the sparse degree of each subregion is consistent as far as possible.
(3) measure the height of the cotton seedling just germinateed latter 5 days and the cotton seedling of Cultivate administration after 30 days respectively, draw the high growth amount of cotton seedling in 25 days, each process is averaged, and calculates rate of increase.
Height × 100% of rate of increase=high growth amount/germination 5 days cotton seedlings.
(4) add up the morbidity cotton seedling number of Cultivate administration after 30 days (cotton seedling having susceptible phenomenon beyond non-health cotton seedling), calculate sickness rate.
Cotton seedling sum × 100% of sickness rate=morbidity cotton seedling number/statistics.
3, result
(1) result of cotton seedling high growth rate is as shown in table 1
Table 1
(2) result of cotton seedling sickness rate is as shown in table 2
Table 2
Because different insect metabolic rules is different, the material and consumption difference that consume can be utilized, the metabolite that metabolism produces is different with movement, therefore, the fertilizer of the inapplicable fresh tobacco leaf gained of different insects process, although the roughly situation of overall nutrition element is similar, but the content of its nutritive element is than different, trace element is different, the more important thing is the metabolite containing various different complexity, and the difference of these materials and nutritive element causes suitability to Different Crop and selectivity.
Experimental result shows, and growing for cotton, the fertilizer efficiency of the biological activity organic fertilizer special utilizing Sarcophga fuscicauda larva process inapplicable fresh tobacco leaf gained fertilizer to prepare is the most remarkable.
embodiment 8 microbe species screens
1, adopt the method for same embodiment 7, explore the impact of different probiotic bacteriums on cotton biological activity organic fertilizer special fertilizer efficiency.Test the combination of each group of microbial germ powder as table 3(or table 4) shown in.
2, result
(1) result of cotton seedling high growth rate is as shown in table 3
Table 3
(2) result of cotton seedling sickness rate is as shown in table 4
Table 4
Main purpose of the present invention is to make full use of inapplicable fresh tobacco leaf resource, utilization of waste material on the one hand, also eliminates the environmental problem that inapplicable fresh tobacco leaf produces on the other hand, realizes turning waste into wealth generation economic benefit simultaneously.According to the research in early stage, utilize housefly, chrysomyia megacephala (big head golden fly) larva, Sarcophga fuscicauda larva, lucilia sericata larvae and black soldier flies larva all can well process inapplicable fresh tobacco leaf, obtain biological organic fertilizer and albumen.Utilization for described biological organic fertilizer is our focus, due to the singularity of tobacco leaf, wherein containing the particular matter being much different from common stalk or ight soil class fertilizer, directly applies to arable farming effect not remarkable.
Research display, need the effect coordinating special plant-growth probiotic bacterium, described biological organic fertilizer could well be applied to as cultivation, and for the screening of probiotic bacterium, we have done a large amount of work, due to the singularity of microorganism strains, otherness between different strains, Survival significant difference in the fertilizer that different strains obtains at different insects process fresh tobacco leaf, and the fertilizer that different insects process fresh tobacco leaf obtains is after different strains effect, wherein the content of material is larger.
Experimental result shows, growing for cotton, and the mixed bacterium powder of azotobacter chroococcum, subtilis and enterobacter cloacae is the best fit probiotic composition of fertilizer special for cotton of the present invention, can reach best fertilizer efficiency.
the ratio optimization of embodiment 9 biological organic fertilizer
1, biological active organic fertilizer is prepared according to the formula of embodiment 1 and preparation method, just for fertilizer formula, by biological organic fertilizer, namely the weight percentage of the fertilizer of the inapplicable fresh tobacco leaf acquisition of Sarcophga fuscicauda larva process is as variable, tested among a small circle by cotton seedling in experimental plot, Zengcheng and screen, explore the proportioning of biological organic fertilizer to the impact of fertilizer efficiency.
2, experimental technique
(1) with the weight percentage of biological organic fertilizer (i.e. the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtain fertilizer) for variable, prepare a series of cotton biological activity organic fertilizer special; Each experimental field subregion 0.5 mu.
(2) conventionally cultivated cotton, evenly spread fertilizer over the fields fertilizer before whole ground, plough soil, make fertilizer side-lower 10 ~ 12 centimeters under soil greatly, rate of fertilizer application is 40 kgs/acre.
Carry out the management of cotton conventional cultivation after seed germination, after germination 5 days time, screening is carried out to cotton seedling and pulls out, the seedling that reservation growing way etc. are consistent as far as possible, obviously inconsistent, excessive, too small seedling is pulled out, meanwhile, ensures that the sparse degree of each subregion is consistent as far as possible.
(3) measure the height of the cotton seedling just germinateed 5 days and the cotton seedling of Cultivate administration after 30 days respectively, draw the high growth amount of cotton seedling in 25 days, each process is averaged, and calculates rate of increase.
Height × 100% of rate of increase=high growth amount/germination 5 days cotton seedlings.
(4) add up the morbidity cotton seedling number of Cultivate administration after 30 days (cotton seedling having susceptible phenomenon beyond non-health cotton seedling), calculate sickness rate.
Cotton seedling sum × 100% of sickness rate=morbidity cotton seedling number/statistics.
3, result
(1) the result display of cotton seedling high growth rate, within the specific limits, along with the raising of fertilizer weight part in cotton biological activity organic fertilizer special formula, the rate of increase of cotton seedling also continues to raise, in the scope of 35 ~ 45 parts, rate of increase is higher, reaches as high as 202%.Further along with the raising of fertilizer weight part, the rate of increase of cotton seedling but demonstrates downward trend.
The reason analyzing above-mentioned phenomenon may be: first because fertilizer can not only provide various nutrient elements and trace element, constantly can also discharge carbonic acid gas, improve the photosynthesis environment of cotton, but also have for probiotic bacterium provides energy etc. to act on, therefore, along with the raising of fertilizer weight part, rapidly, rate of increase keeps constantly rising in cotton seedling growth.But when the ratio of fertilizer is too high, can causes lack of water in soil, support imbalance, crop root water suction difficulty, also there is the unbalanced problem of nutrition, therefore too high Organic manure content can hinder growing of cotton seedling on the contrary.
(2) the result display of cotton seedling sickness rate, within the specific limits, along with the raising of fertilizer weight part in cotton biological activity organic fertilizer special formula, cotton seedling sickness rate presents the trend first declining and raise, in the scope of 35 ~ 45 parts, sickness rate is lower, 1.9 ~ 5.0%.
In sum, in order to reach the overall benefit of culture of cotton, when in cotton biological activity organic fertilizer special formula of the present invention, the weight part of fertilizer is 35 ~ 45 parts better, the best is 40 parts.
the ratio optimization of embodiment 10 microbial germ powder
1, with the method for embodiment 9, explore the proportioning of microbial germ powder to the impact of fertilizer efficiency, just variable is changed into the weight part of microbial germ powder in cotton biological activity organic fertilizer special formula.
2, result
(1) the result display of cotton seedling high growth rate, within the specific limits, along with the raising of microbial germ powder weight part in cotton biological activity organic fertilizer special formula, the rate of increase of cotton seedling also continues to raise, in the scope of 2 ~ 6.0 parts, rate of increase is higher, reaches as high as 202%.Further along with the raising of microbial germ powder weight part, the rate of increase of cotton seedling but demonstrates downward trend.
(2) the result display of cotton seedling sickness rate, within the specific limits, along with the raising of microbial germ powder weight part in cotton biological activity organic fertilizer special formula, cotton seedling sickness rate presents the trend first declining and stagnate and even slowly raise, in the scope of 2.5 ~ 7.0 parts, sickness rate is lower, 2.0 ~ 5.1%.
In sum, in order to reach the overall benefit of culture of cotton, in cotton biological activity organic fertilizer special formula of the present invention, weight part the best of microbial germ powder is 2.5 ~ 6.0 parts, and the best is 5 parts.
embodiment 11 field production experiment among a small circle
1, between (1) 2013 year to 2014 in Weifang City of Shandong Province, with embodiment 1 ~ 3 prepare cotton biological activity organic fertilizer special carried out culture of cotton experiment in experimental plot.
(2) positive control: commercial Stanley ammonium potassium phosphate composite fertilizer.
Negative control: the cotton biological activity organic fertilizer special not adding microorganism, the fertilizer namely using merely the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process to obtain coordinates inorganic fertilizer, and concrete formula is: the fertilizer 40 parts that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains, microbial germ powder 4 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts.
(3) examination cotton variety is supplied to be that Shandong cotton grinds No. 28.
2, fertilising and cotton cultivation method are with embodiment 9, and rate of fertilizer application is 40kg/ mu.
3, field experiment result is as shown in table 5, data presentation, compared with positive control, uses cotton biological activity organic fertilizer special stimulation ratio of the present invention substantially more than 10%, obvious effect of increasing production.
And the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains is without the effect of any microorganism, directly coordinates with inorganic fertilizer, well can not be applied to the fertilising of crop.
The effect of increasing production of table 5 embodiment 1 ~ 3 fertilizer compared with the control

Claims (10)

1. one to grow cotton biological activity organic fertilizer special, it is characterized in that, the fertilizer obtained by the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process and microbial germ powder, then be equipped with inorganic salt fertilizer and prepare; Described microbial germ powder be vinelandii, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
2. cotton biological activity organic fertilizer special according to claim 1, is characterized in that, be made up of the component of following weight part: biological organic fertilizer 35 ~ 45 parts, microbial germ powder 2.5 ~ 6.0 parts, calcium superphosphate 3 ~ 4 parts, potassium sulfate 7 ~ 15 parts, potassium primary phosphate 1.5 ~ 3.2 parts, 1 ~ 2 part, calcium sulfate, 0.2 ~ 1 part, zinc sulfate, ammonium molybdate 1.3 ~ 2.9 parts, 3.2 ~ 4.8 parts, iron(ic) chloride, clay 5 ~ 15 parts, borax 10 ~ 15 parts;
Described biological organic fertilizer is the fertilizer that the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process obtains;
The vinelandii of described microbial germ powder to be weight ratio be 1 ~ 3:1:0.5, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
3. cotton biological activity organic fertilizer special according to claim 2, it is characterized in that, each ingredients weight parts of described cotton biological activity organic fertilizer special is: biological organic fertilizer 40 ~ 45 parts, microbial germ powder 4 ~ 5.5 parts, calcium superphosphate 3 ~ 4 parts, potassium sulfate 10 ~ 15 parts, potassium primary phosphate 1.5 ~ 2 parts, 1.5 parts, calcium sulfate, 0.5 ~ 0.8 part, zinc sulfate, ammonium molybdate 2 ~ 2.5 parts, 3.5 ~ 4 parts, iron(ic) chloride, clay 8 ~ 10 parts, borax 10 ~ 12 parts;
The vinelandii of described microbial germ powder to be weight ratio be 1.5:1:0.5, subtilis ( bacillus subtilis) and enterobacter cloacae ( enterobacter cloacae) mixed bacterium powder.
4. cotton biological activity organic fertilizer special according to claim 2, it is characterized in that, each ingredients weight parts of described cotton biological activity organic fertilizer special is: biological organic fertilizer 40 parts, microbial germ powder 5 parts, calcium superphosphate 3.5 parts, potassium sulfate 13 parts, potassium primary phosphate 2 parts, 1.5 parts, calcium sulfate, 0.5 part, zinc sulfate, ammonium molybdate 2 parts, 4 parts, iron(ic) chloride, clay 9 parts, borax 12 parts.
5., according to the arbitrary described cotton biological activity organic fertilizer special of claims 1 to 3, it is characterized in that, described vinelandii be azotobacter chroococcum ( azotobacter chroococum) or India Bai Yelin kirschner bacterium ( beijerinckia indica).
6. cotton biological activity organic fertilizer special according to claim 5, is characterized in that, described vinelandii be azotobacter chroococcum ( azotobacter chroococum).
7. the preparation method of 1 ~ 6 arbitrary described cotton biological activity organic fertilizer special described in claim 1, it is characterized in that, step is as follows:
S1. the inapplicable fresh tobacco leaf of Sarcophga fuscicauda larva process is utilized to obtain fertilizer;
S2. proportionally, after calcium superphosphate, potassium sulfate, potassium primary phosphate, calcium sulfate, zinc sulfate, ammonium molybdate, iron(ic) chloride, clay, borax are carried out fragmentation, mix thoroughly;
S3., after the particle obtained by S2 again and biological organic fertilizer mix, below moisture to 5% is dried;
S4. the material after drying and microbial germ powder mix, and granulation, obtains cotton biological activity organic fertilizer special.
8. preparation method according to claim 7, it is characterized in that, the method for step S1 is:
S11. collect the fragment that inapplicable fresh tobacco leaf is chopped into particle diameter 2 ~ 3cm, add auxiliary material and stir, be mixed with culture material;
S12. culture material surface access Sarcophga fuscicauda newly hatched larvae described in S11;
S13. Sarcophga fuscicauda newly hatched larvae started bio-transformation culture material in accessing the same day;
Taking food S14. through Sarcophga fuscicauda larva 4 ~ 5 days, culture material changes biological organic fertilizer into; Sarcophga fuscicauda larva reaches aging state.
9. the cotton biological activity organic fertilizer special for preparing of preparation method described in claim 7.
10. the application of the arbitrary described cotton biological activity organic fertilizer special of claim 1 ~ 6 in cotton is cultivated.
CN201510094124.5A 2015-03-03 2015-03-03 One grows cotton biological activity organic fertilizer special and its application Expired - Fee Related CN104692972B (en)

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