CN104686116A - Topping and cutting propagation method for energy crop--silvergrass - Google Patents
Topping and cutting propagation method for energy crop--silvergrass Download PDFInfo
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- CN104686116A CN104686116A CN201310648126.5A CN201310648126A CN104686116A CN 104686116 A CN104686116 A CN 104686116A CN 201310648126 A CN201310648126 A CN 201310648126A CN 104686116 A CN104686116 A CN 104686116A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to a topping and cutting propagation method for an energy crop--silvergrass. The method comprises the following steps: topping a mother plant before cutting propagation of the silvergrass and cultivating first-grade branches; after the first-grade branches form, eliminating growth points of the first-grade branches to promote formation of second-grade branches; with the mother plant and the first-grade branches with the second-grade branches as materials, carrying out mother plant selection, cutting cultivation, seedbed preparation, cutting preparation, cuttage, management in a seedling stage, seedling lifting and transplanting. Thus, a highly-efficient and stable cutting propagation method for the silvergrass is constructed. The method provided by the invention can greatly increase seedling sources of the energy crop--the silvergrass; meanwhile, the method has the advantages of high propagation coefficient, capacity of realizing good consistency of the genetic basis of cutting seedlings and obtaining strong roots, high survival rate of transplanting and low propagation cost, and is a simple, convenient and effective seedling raising method which is suitable for commercial production of fine silvergrsss strains.
Description
Technical field
The present invention relates to a kind of energy crop---reed (Miscanthus scchariflora (Maxim.) Nakai) Technique on Cuttage Propagation of pinching, specifically reed plant that life is healthy and strong is then carried out to the second branch obtained after secondary is pinched needed for cuttage, it is pruned and after culture of rootage, completes and numerous increment is expanded to material.Lower or the unhandy problem of current reed reproduction coefficient is solved by this top propagation technique; the stem stalk of every Cong Di (about 10 stem stalks) can obtain 200 ~ 300 cuttage seeding; be much higher than currently reported reproduction coefficient (4-8); the reproductive-cost of obvious reduction reed seedling, realizes the seeling industry of the scale of energy crop reed, commercialization, low cost.Belong to biological technical field.
Background technology
The energy is the important guarantee of socio-economic development, develops the Renewable Energy Resources that can not bring harm to environment, seems particularly urgent.Within 2007, No.1 Central File explicitly points out, with the Biomass Industry that bioenergy, biomass product and biomass material are main, it is the rising industry expanded Agricultural Function, promote resources effective utilization, and encourage place with good conditionsi to utilize the resource such as deserted mountain, wasteland, Developing Biomass crop cultivating and growing.Living beings vegetable material main at present comprises woody plant, annual and herbaceos perennial (Hoogwijk M, Faaija A, van den Broek R, Berndcs G, Gieien D, Turkenburg W.Exploration of the ranges of the global potential of biomass for energy.Biomass and Bioenergy, 2003,25 (2): 119-133).Wherein woody plant growth cycle is long, and annual herb planting cost is high, be all not suitable for carrying out large-scale promotion utilization (Shi Yuanchun. talk the Railway Project in Biomass Industry Development of China. Chinese basic science, 2005 (6): 326).And herbaceos perennial has the following advantages as energy-source plant tool: regeneration capacity is strong, once plants, can long term benefit; Maturation is fast, dry matter production is high, photosynthetic efficiency is high, resistance; Be conducive to protection of the environment, maintain the ecological balance; Do not have the danger of nuclear energy and wind energy, tide energy and geothermoenergy limitation (Zhu Xifeng, Zheng Jilu, Lu Qiang, etc. biomass pyrolysis liquefaction device Research and test is studied. Chinese engineering science, 2006,8 (10): 89-93).Therefore research and development perennial herb energy-source plant is necessary, for bio-energy industry provides reliable raw material to ensure.
Reed (M.sacchariflora (Maxim.) Nakai) is grass family awns genus (M.Anderss.) dispersed species, perennial tall and big C4 draft, originate in East Asia, China be awns platymiscium distribution of resource center (Chen Shouliang. Chinese Plants will. Beijing: Science Press, 1997,10 (2): 19).Reed has less investment, adapts to the feature strong, yield of biomass is high, combustion characteristics good and regeneration capacity is strong, desirable draft energy-source plant (Lewandowski I, Heinz A.Delayed harvest of miscanthus-influences on biomass quantity and quality and environmental impacts of energy production.Eur.J.Agron., 2003,19:45-63).Along with implementing and development of national development strategy and a series of new forms of energy strategy, following China will be huge for the demand of biological energy raw material.
Reed both can pass through seminal propagation, also can pass through vegetative reproduction.Although by seminal propagation advantage of lower cost, but due to its gene height heterozygosis, there is serious separation in the offspring of seminal propagation, must cause that the growth of reed colony is very different, yield and quality declines, and utilize the nourish and generate purity and characteristic that can well ensure reed elite germplasm, farthest can ensure the quality of seedling and the output after planting.But traditional subterranean stem division propagation method reproduction coefficient is low, carries underground damage by disease and insect simultaneously, operation inconvenience, cost is relatively high, cannot meet the large-scale production needs of reed as energy-source plant crop reed.To nourish and generate coefficient to improve reed further, both at home and abroad from the nineties in 20th century, just has relevant report successively to the research that reed has carried out tissue culture rapid propagating technology, respectively by seed, stem stalk, young fringe etc. for explant establishes reed tissue culture quick breeding system, the numerous coefficient of the expansion that different explants reaches is different, and it is numerous most effective soon to utilize young fringe to carry out, but its reproduction coefficient is not also more than 4.And the cottage propagation research aspect of reed, Agricultural University Of Hunan for material, establishes its cottage propagation system with raw reed stem section then, although its planting percent can reach 95%, but because it directly utilizes raw stem stalk to be then cuttage material, axillalry bud limited amount, therefore its reproduction coefficient is not also more than 8.
Said method all has respective feature, method for tissue culture operation is inconvenient, facility requirements is relatively high, and cuttage breeding, although operation relative ease, facility requirements are simple, but reproduction coefficient is not high, the demand of reed large scale application really cannot be solved.The present invention is pinched by reed and cultivates one-level branch, further the pinch technology of induction second branch and carry out cottage propagation; not only can keep the good characteristic of maternal plant; and its reproduction coefficient is high; the stem stalk of every Cong Di (about 10 stem stalks) can obtain 200 ~ 300 cuttage seeding; be much higher than currently reported reproduction coefficient; the reproductive-cost of obvious reduction reed seedling, thus realize the seeling industry of the scale of energy crop reed, commercialization, low cost.
Summary of the invention
The object of the invention is to set up energy crop---the efficient Technique on Cuttage Propagation system of reed, it is high that this system has reproduction coefficient, and reproductive-cost is low, easy and simple to handle, well solves the problem of seeling industry of the scale of energy crop reed, commercialization, low cost.
The pinch concrete steps of cuttage breeding method of energy crop provided by the invention---reed are as follows:
(1) maternal plant is selected to cultivate with cuttings
In annual 6 ~ September, choose perennial energy crop---reed is raw healthy and strong plant crop maternal plant then, utilize secateurs to pinch to it and cultivate one-level branch, pinch to promote that second branch is formed to it when one-level branch growth to 40 ~ 50cm, utilize the method on reed plant, obtain second branch needed for cuttage.
(2) matrix prepares and seedbed arrangement
Matrix is configured according to the volume ratio of 2: 2: 1 with garden mould, sand, peat, the matrix working concentration of every cube of mixing is that 25% wetting powder, 1000 times of liquid triazolone solution 200 ~ 300L carry out sterilizing to ground, seedbed matrix, the necessary draining in ground, seedbed is good, and stromal thickness is not less than 10cm.
(3) cuttings prepares
One-level branch with second branch is tiltedly cut into the long cuttings material comprising a second branch of 6 ~ 10cm, blades all in cuttings and leaf sheath is removed except the lobus cardiacus retained on second branch, after soaking in root-growing agent (comprising methyl α-naphthyl acetate (NAA) 50mg/L and indolebutyric acid (IBA) 10mg/L) solution, dry for subsequent use, the top tip avoiding injuring the tender second branch of children will be noted in cuttings set-up procedure.
(4) cuttage technique
Cuttage is carried out in greenhouse or on the cutting bed with moisturizing facility, cutting density is 4cm × 4cm, the degree of depth is advisable just above second branch base portion and one-level branch junction (generally with 2 ~ 3cm) with matrix, 1 cuttage is answered to put in place during cuttage, plug is repeatedly avoided to affect cuttings quality and survival rate, want timely spraying and moisturizing after cuttage completes, spray first to soak completely with soil and be advisable.
(5) management after inserting
With broad spectrum type antibacterial agent carbendazim 1200 times of liquid, spray disinfection, moisturizing are carried out to slotting machine and cuttings after cuttage, in follow-up one week, suitable shading is carried out to slotting machine, the spraying of more than 3 times is carried out every day to maintain the moistening of slotting machine depending on weather condition, second week removes the facility that shades on slotting machine, at least carry out a spraying and moisturizing every day depending on weather condition, with ensure cuttings can Rapid Rooting, survival.
(6) transplantation of seedlings is played
After cuttage completes 3 weeks, the cuttings of having taken root in slotting machine tripped out, the one-level compound stem section inserted together when must remove cuttage during lifting, preferably carries out balled transplanting, can for the production of application after transplanting slow seedling.
Outstanding feature of the present invention creates from internal cause external cause and two aspects the developmental condition being conducive to promoting reed cuttage root-taking seedling, and based on prior art reproduction coefficient feature on the low side, works out to utilize second branch to carry out cuttage to expand numerous technical system.Internal cause is the life then of selection reed, nutrient accumulation enriches, fully-developed stem stalk, external cause is mainly according to existing propagation technique feature, by sprouting of lateral bud after pinching to reed secondary, take root, the research of seedling correlation, create the growing environment that water, gas and nutrient that reed is applicable to are coordinated, thus establish reed rapid propagation system that reproduction coefficient is multiplied.
The comprehensive planting percent of reed of this method cuttage reaches 96.03%; reproduction coefficient high (being up to 30); reproductive-cost is low; easy and simple to handle; well solve energy crop reed and carry out the problem that in large-area applications popularization, high quality seedling lacks, for reed plant husbandry establishes the seedling breeding method of scale, commercialization, low cost.
Embodiment
Embodiment 1:
Late June, with 10 rhizome stalks of robust growth for maternal plant, utilize secateurs to its cultivation (axillalry bud) one-level branch of pinching, through the microscler one-tenth of 6 Zhousheng 51 one-level branches, its length is between 40 ~ 50cm, and now pinching to one-level branch promotes that second branch is formed.After secondary is pinched 3 weeks, form second branch 248, one-level branch with second branch is tiltedly cut into the cuttings material that 6 ~ 10cm is long, comprise a second branch, blades all in cuttings and leaf sheath is removed except the lobus cardiacus retained on second branch, after utilizing the solution of methyl α-naphthyl acetate (NAA) 50mg/L and indolebutyric acid (IBA) 10mg/L to soak cuttings base portion, dry for subsequent use.Matrix is configured according to the volume ratio of 2: 2: 1 with garden mould, sand, peat, and the matrix working concentration of every cube of mixing is that 25% wetting powder, 1000 times of liquid triazolone solution 200 ~ 300L carry out sterilizing to ground, seedbed matrix.By cuttings cuttage in greenhouse on cutting bed, cutting density is 4cm × 4cm, and the degree of depth is advisable just above second branch base portion and one-level branch junction (generally with 2 ~ 3cm) with matrix, spraying and moisturizing after cuttage completes.With broad spectrum type antibacterial agent carbendazim 1200 times of liquid, spray disinfection, moisturizing are carried out to slotting machine and cuttings, slotting machine is suitably shaded one week, carry out 3 spraying and moisturizing every day, second week removes the facility that shades on slotting machine, at least carries out a spraying and moisturizing every day depending on weather condition.After cuttage completes 21 days, the one-level compound stem section inserted together when the cuttings of having taken root in slotting machine being tripped out and remove cuttage, preferably carries out balled transplanting.Cuttage cuttings amounts to 248, seedling of taking root 239 strain, and planting percent is 96.37%, and reproduction coefficient is 23.9.
Embodiment 2:
Early August, with 10 rhizome stalks of robust growth for maternal plant, utilize secateurs to its cultivation (axillalry bud) one-level branch of pinching, through the microscler one-tenth of 5 Zhousheng 56 one-level branches, its length is between 40 ~ 50cm, and now pinching to one-level branch promotes that second branch is formed.After secondary is pinched 3 weeks, form second branch 276, one-level branch with second branch is tiltedly cut into the cuttings material that 6 ~ 10cm is long, comprise a second branch, blades all in cuttings and leaf sheath is removed except the lobus cardiacus retained on second branch, after utilizing the solution of methyl α-naphthyl acetate (NAA) 50mg/L and indolebutyric acid (IBA) 10mg/L to soak cuttings base portion, dry for subsequent use.Matrix is configured according to the volume ratio of 2: 2: 1 with garden mould, sand, peat, and the matrix working concentration of every cube of mixing is that 25% wetting powder, 1000 times of liquid triazolone solution 200 ~ 300L carry out sterilizing to ground, seedbed matrix.By cuttings cuttage in greenhouse on cutting bed, cutting density is 4cm × 4cm, and the degree of depth is advisable just above second branch base portion and one-level branch junction (generally with 2 ~ 3cm) with matrix, spraying and moisturizing after cuttage completes.Carry out spray disinfection, moisturizing with broad spectrum type antibacterial agent carbendazim 1200 times of liquid to slotting machine and cuttings, suitably shade one week to slotting machine, carry out 4 ~ 5 spraying and moisturizing every day, second week removes the facility that shades on slotting machine, carries out 2 spraying and moisturizing every day depending on weather condition.After cuttage completes 3 weeks, the one-level compound stem section inserted together when the cuttings of having taken root in slotting machine being tripped out and remove cuttage, preferably carries out balled transplanting.Cuttage cuttings amounts to 276, seedling of taking root 267 strain, and planting percent is 96.74%, and reproduction coefficient is 26.7.
Claims (8)
1. energy crop---reed is pinched cuttage breeding method.It is characterized in that, comprise the steps:
(1) maternal plant is selected to cultivate with cuttings
Choose perennial energy crop---reed is raw healthy and strong plant crop maternal plant then, it is pinched and cultivates one-level branch, after branch is formed, again pinch to promote that second branch is formed to the one-level branch formed, utilize the method on reed plant, obtain second branch needed for cuttage.
(2) matrix prepares and seedbed arrangement
Matrix mixes with garden mould, sand, peat, and the matrix after mixing uses soil fungicides to carry out sterilizing to ground, seedbed matrix, and the necessary draining in ground, seedbed is good, and stromal thickness is not less than 10cm.
(3) cuttings prepares
One-level branch with second branch is tiltedly cut into the cuttings material comprising a second branch, blades all in cuttings and leaf sheath is removed except the lobus cardiacus retained on second branch, after soaking in root-growing agent (comprising methyl α-naphthyl acetate and the indolebutyric acid of variable concentrations) solution, dry for subsequent use, the top tip avoiding injuring the tender second branch of children will be noted in cuttings set-up procedure.
(4) cuttage technique
Cuttage is carried out in greenhouse or on the cutting bed with moisturizing facility, cutting density is 4cm × 4cm, the degree of depth is advisable just above second branch base portion and one-level branch junction with matrix, 1 cuttage is answered to put in place during cuttage, plug is repeatedly avoided to affect cuttings quality and survival rate, want timely spraying and moisturizing after cuttage completes, spray first to soak completely with soil and be advisable.
(5) management after inserting
With bactericide, spray disinfection, moisturizing are carried out to slotting machine and cuttings after cuttage, in follow-up one week, suitable shading is carried out to slotting machine, the spraying of more than 3 times is carried out every day to maintain the moistening of slotting machine depending on weather condition, second week removes the facility that shades on slotting machine, at least carry out a spraying and moisturizing every day depending on weather condition, with ensure cuttings can Rapid Rooting, survival.
(6) transplantation of seedlings is played
After cuttage completes 3 weeks, the cuttings of having taken root in slotting machine is tripped out, then transplanting can for the production of application.
2. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, it is characterized in that, maternal plant described in step (1), one-level branch are pinched and all utilize secateurs to carry out, maternal plant is pinched and monthlyly to carry out 6 ~ 9, and one-level branch growth is pinched and to be carried out when it grows into 40 ~ 50cm.
3. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, it is characterized in that, matrix described in step (2) presses garden mould: husky: peat=2: the volume ratio configuration of 2: 1, the matrix working concentration of every cube of mixing is that 25% wetting powder, 1000 times of liquid triazolone solution 200L carry out disinfection.
4. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, it is characterized in that, the cuttings described in step (3) is the long one-level branch comprising a second branch of 6 ~ 10cm.
5. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, and it is characterized in that, the root-growing agent described in step (3) comprises methyl α-naphthyl acetate (NAA) 50mg/L and indolebutyric acid (IBA) 10mg/L.
6. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, and it is characterized in that, the cuttage degree of depth described in step (4) is generally advisable with 2 ~ 3cm.
7. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, and it is characterized in that, the bactericide described in step (5) is that broad spectrum type antibacterial agent carbendazim 1200 times of liquid spray.
8. a kind of energy crop according to claim 1---reed is pinched cuttage breeding method, it is characterized in that, the lifting described in step (6), the one-level compound stem section inserted together when must remove cuttage when transplanting, preferably carry out balled transplanting.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105165363A (en) * | 2015-09-30 | 2015-12-23 | 江苏农林职业技术学院 | High-yield seed multiplication method for talinum crassifolium |
| CN107912160A (en) * | 2017-12-28 | 2018-04-17 | 广东美景园林建设有限公司 | A kind of method of the cottage breeding of mangrove acanthus |
| CN108012683A (en) * | 2017-11-29 | 2018-05-11 | 广州市新茂生态农业发展有限公司 | A kind of red autumnal leaves podocarpus blade cuttage breeding method |
| CN109716944A (en) * | 2019-03-18 | 2019-05-07 | 中国农业科学院麻类研究所 | A kind of ramie cuttage breeding method |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105165363A (en) * | 2015-09-30 | 2015-12-23 | 江苏农林职业技术学院 | High-yield seed multiplication method for talinum crassifolium |
| CN105165363B (en) * | 2015-09-30 | 2017-12-08 | 江苏农林职业技术学院 | A kind of high yield breeding method of Talinum crassifolium |
| CN108012683A (en) * | 2017-11-29 | 2018-05-11 | 广州市新茂生态农业发展有限公司 | A kind of red autumnal leaves podocarpus blade cuttage breeding method |
| CN107912160A (en) * | 2017-12-28 | 2018-04-17 | 广东美景园林建设有限公司 | A kind of method of the cottage breeding of mangrove acanthus |
| CN109716944A (en) * | 2019-03-18 | 2019-05-07 | 中国农业科学院麻类研究所 | A kind of ramie cuttage breeding method |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
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