CN104671852A - Polypeptide-containing biofertilizer for organic Chinese cabbages - Google Patents
Polypeptide-containing biofertilizer for organic Chinese cabbages Download PDFInfo
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- CN104671852A CN104671852A CN201310639904.4A CN201310639904A CN104671852A CN 104671852 A CN104671852 A CN 104671852A CN 201310639904 A CN201310639904 A CN 201310639904A CN 104671852 A CN104671852 A CN 104671852A
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Abstract
The invention discloses a polypeptide-containing biofertilizer for organic Chinese cabbages, which is characterized by comprising the following components in parts by weight: 5-20 parts of hydrolytic active polypeptide, 10-20 parts of composite microbial inoculant, 40-50 parts of grass carbon and 20-30 parts of inorganic additive. The preparation method of the hydrolytic active polypeptide comprises the following steps: mixing bean pulp and a 95% sulfuric acid water solution in a mass ratio of 1:2, hydrolyzing at 80-90 DEG C for 24-48 hours, adding ammonium bicarbonate to regulate the pH value to 2-5, filtering to obtain a filtrate, concentrating the filtrate, and carrying out freeze-drying to obtain the powdery hydrolytic active polypeptide. The biofertilizer is low in price, obviously enhances the fertilizer efficiency of the microbial fertilizer, promotes the healthy and vigorous growth of living organisms, is especially suitable for culture of organic Chinese cabbages, and greatly enhances the Chinese cabbage yield.
Description
Technical field
The invention belongs to agricultural fertilizer field, be specifically related to a kind of for organic Chinese cabbage containing polypeptide bio-feritlizer.
Background technology
Along with improving constantly of standard of living, the safety requirements of human consumer to vegetables is also more and more higher, does not use the demand of the organic vegetable of conventional chemical fertilizers also to get more and more.Compare with traditional fertilizer, microbial fertilizer at raising utilization rate of fertilizer, increase crop yield and there is obvious advantage in preserving the ecological environment.Carrying out microbial fertilizer research in a deep going way, having very important significance for realizing agricultural sustainable development.
The compound that two or more amino acid is connected with peptide bond, is called peptide.General 100 compounds be connected with upper amino acid are called protein, and the connected compound of 2-100 amino acid is called polypeptide.Polypeptide biological activity is high, and it can regulate various physiological activity and biochemical reaction.To organism, there is very important irreplaceable regulating effect.Developed country is devoted to, in the research and development of biological polypeptide fertilizer, to obtain good effect in recent years.There are some researches show, soybean polypeptide foliage spray and root perfusion applying mode process Rumex gmelini Turcz., wherein have a significant impact the output increase of resveratrol in root.There are some researches show, use the red bayberry of Lv Feng source polypeptide, peach, Lee, cherry pol all than do not use be improved significantly, soluble solid increases by 9.6%, 0.4%, 2.4%, 12.9% respectively.Lv Feng source polypeptide synergistic urea field test shows, wheat quality improves obviously, thousand seed weight, wet gluten, unit weight, crude protein decile you can well imagine high 0.4g, 6.87%, 7g/L, 2.22%; Corn silking does sth. in advance 2 ~ 4d, and breeding time, the bald point of fringe reduced in advance; Cotton shifts to an earlier date 2 ~ 5d and blooms, and becomes bell number increase by 11% ~ 14.4% etc.In addition, poly aspartic acid (being called for short PASP) is a kind of water-soluble polypeptide, the polypeptide of the fertilizer that is often used in agriculture at present, and PASP contains carboxyl, rapidly and nutrient ionic bond exchanging with the H+ that crop root is secreted, the process that nutrient ion is absorbed by crops can be completed.PASP improves utilization rate of fertilizer, rehabilitating soil pollutes and improve soil nutrient availability.
At present, though there is the multiple polypeptide raw material for polypeptide fertilizer, its price is generally expensive, causes the costliness of fertilizer price, and the use for common peasant household has very large restriction, can not be widely used.
Summary of the invention
The object of the present invention is to provide a kind of for organic Chinese cabbage containing polypeptide bio-feritlizer, this fertilizer contains the polypeptide active substance by degradation of soybean meal, cheap, promotes that the health of living organism is grown up strong and sturdy, is particularly useful for the cultivation of organic Chinese cabbage.
Technical scheme of the present invention is as follows:
For organic Chinese cabbage containing a polypeptide bio-feritlizer, comprise following component by weight: hydrolytic activity polypeptide 5-20 part, complex micro organism fungicide 10-20 part, peat composed of rotten mosses 40-50 part, inorganic additives 20-30 part;
Wherein, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add bicarbonate of ammonia adjust pH to 2-5 after 24-48 hour, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
In the present invention, described complex micro organism fungicide comprises two or more the mixture in subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium).
In the present invention, in described complex micro organism fungicide, living bacteria count is not less than 5x10
8cfu.
In the present invention, described inorganic additives is two or more the mixture in urea, potassiumphosphate, diammonium phosphate, potassium sulfate, magnesium sulfate, zinc sulfate.
Beneficial effect of the present invention: bio-feritlizer of the present invention contains the polypeptide active substance obtained by degradation of soybean meal, its protein raw materials is easy to get, cheap, significantly reduces the cost of fertilizer, is of value to production estimation person widely and uses.Bio-feritlizer of the present invention obviously increases the fertilizer efficiency of microbial fertilizer, promotes that the health of living organism is grown up strong and sturdy, is particularly useful for the cultivation of organic Chinese cabbage, increases considerably Chinese cabbage output.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
For organic Chinese cabbage containing a polypeptide bio-feritlizer, comprise following component by weight: hydrolytic activity polypeptide 5 parts, complex micro organism fungicide 10 parts, the peat composed of rotten mosses 40 parts, inorganic additives 200 parts;
Wherein, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add bicarbonate of ammonia adjust pH to 5 after 24 hours, filter and obtain filtrate, filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
Described complex micro organism fungicide comprises subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium), the mixture of 1:1:1:1 mixing in mass ratio, in mixture, living bacteria count is not less than 5x10
8cfu.
Containing 10% urea, 5% potassiumphosphate, 10% diammonium phosphate, 4% potassium sulfate, 10% magnesium sulfate, 10% zinc sulfate in described inorganic additives.
Embodiment 2
For organic Chinese cabbage containing a polypeptide bio-feritlizer, comprise following component by weight: hydrolytic activity polypeptide 15 parts, complex micro organism fungicide 20 parts, the peat composed of rotten mosses 50 parts, inorganic additives 30 parts;
Wherein, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add bicarbonate of ammonia adjust pH to 4 after 35 hours, filter and obtain filtrate, filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
Described complex micro organism fungicide comprises subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium), the mixture of 1:1:1:1 mixing in mass ratio, in mixture, living bacteria count is not less than 5x10
8cfu.
Containing 10% urea, 5% potassiumphosphate, 10% diammonium phosphate, 4% potassium sulfate, 10% magnesium sulfate, 10% zinc sulfate in described inorganic additives.
Claims (4)
1. for organic Chinese cabbage containing a polypeptide bio-feritlizer, it is characterized in that, comprise following component by weight: hydrolytic activity polypeptide 5-20 part, complex micro organism fungicide 10-20 part, peat composed of rotten mosses 40-50 part, inorganic additives 20-30 part;
Wherein, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add bicarbonate of ammonia adjust pH to 2-5 after 24-48 hour, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
2. according to claim 1 for organic Chinese cabbage containing polypeptide bio-feritlizer, it is characterized in that, described complex micro organism fungicide comprises two or more the mixture in subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium).
3. according to claim 1 and 2 for organic Chinese cabbage containing polypeptide bio-feritlizer, it is characterized in that, in described complex micro organism fungicide, living bacteria count is not less than 5x10
8cfu.
4. according to claim 1 for organic Chinese cabbage containing polypeptide bio-feritlizer, it is characterized in that, described inorganic additives is two or more the mixture in urea, potassiumphosphate, diammonium phosphate, potassium sulfate, magnesium sulfate, zinc sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639904.4A CN104671852A (en) | 2013-11-29 | 2013-11-29 | Polypeptide-containing biofertilizer for organic Chinese cabbages |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310639904.4A CN104671852A (en) | 2013-11-29 | 2013-11-29 | Polypeptide-containing biofertilizer for organic Chinese cabbages |
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|---|---|
| CN104671852A true CN104671852A (en) | 2015-06-03 |
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ID=53307505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310639904.4A Pending CN104671852A (en) | 2013-11-29 | 2013-11-29 | Polypeptide-containing biofertilizer for organic Chinese cabbages |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106396859A (en) * | 2016-08-28 | 2017-02-15 | 广西平乐农药厂 | Liquid fertilizer applied in Chinese cabbage planting process |
| CN109485521A (en) * | 2018-12-03 | 2019-03-19 | 上海农乐生物制品股份有限公司 | A kind of organic fertilizer and preparation method thereof of plant protein peptide compounding |
-
2013
- 2013-11-29 CN CN201310639904.4A patent/CN104671852A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106396859A (en) * | 2016-08-28 | 2017-02-15 | 广西平乐农药厂 | Liquid fertilizer applied in Chinese cabbage planting process |
| CN109485521A (en) * | 2018-12-03 | 2019-03-19 | 上海农乐生物制品股份有限公司 | A kind of organic fertilizer and preparation method thereof of plant protein peptide compounding |
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Application publication date: 20150603 |