CN104671962A - Polypeptide-substance-containing microbial fertilizer - Google Patents

Polypeptide-substance-containing microbial fertilizer Download PDF

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Publication number
CN104671962A
CN104671962A CN201310638649.1A CN201310638649A CN104671962A CN 104671962 A CN104671962 A CN 104671962A CN 201310638649 A CN201310638649 A CN 201310638649A CN 104671962 A CN104671962 A CN 104671962A
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CN
China
Prior art keywords
fertilizer
polypeptide
microbial fertilizer
containing peptide
material according
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Pending
Application number
CN201310638649.1A
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Chinese (zh)
Inventor
崔国臣
崔昌娜
顾艳丽
周悦
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DALIAN SANKE BIO-ENGINEERING Co Ltd
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DALIAN SANKE BIO-ENGINEERING Co Ltd
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Priority to CN201310638649.1A priority Critical patent/CN104671962A/en
Publication of CN104671962A publication Critical patent/CN104671962A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a polypeptide-substance-containing microbial fertilizer. The invention is characterized in that the microbial fertilizer is composed of the following components in percentage by weight: 5-15% of hydrolytic active polypeptide, 3-8% of composite microbial inoculant, 40-60% of organic fertilizer and 20-37% of inorganic fertilizer. The polypeptide-substance-containing microbial fertilizer contains the polypeptide active substances degraded from animal and plant protein raw materials. The protein raw materials are accessible and low in price, thereby greatly lowering the cost of the fertilizer and being beneficial to wide crop production. The polypeptide-substance-containing microbial fertilizer can obviously enhance the fertilizer efficiency of the microbial fertilizer, promote the healthy and vigorous growth of the living organisms and greatly promote the crop yield increase.

Description

A kind of containing peptide material microbial fertilizer
Technical field
The invention belongs to agricultural fertilizer field, be specifically related to a kind of containing peptide material microbial fertilizer.
Background technology
The compound that two or more amino acid is connected with peptide bond, is called peptide.General 100 compounds be connected with upper amino acid are called protein, and the connected compound of 2-100 amino acid is called polypeptide.Polypeptide biological activity is high, and it can regulate various physiological activity and biochemical reaction.To organism, there is very important irreplaceable regulating effect.Developed country is devoted to, in the research and development of biological polypeptide fertilizer, to obtain good effect in recent years.There are some researches show, soybean polypeptide foliage spray and root perfusion applying mode process Rumex gmelini Turcz., wherein have a significant impact the output increase of resveratrol in root.There are some researches show, use the red bayberry of Lv Feng source polypeptide, peach, Lee, cherry pol all than do not use be improved significantly, soluble solid increases by 9.6%, 0.4%, 2.4%, 12.9% respectively.Lv Feng source polypeptide synergistic urea field test shows, wheat quality improves obviously, thousand seed weight, wet gluten, unit weight, crude protein decile you can well imagine high 0.4g, 6.87%, 7g/L, 2.22%; Corn silking does sth. in advance 2 ~ 4d, and breeding time, the bald point of fringe reduced in advance; Cotton shifts to an earlier date 2 ~ 5d and blooms, and becomes bell number increase by 11% ~ 14.4% etc.In addition, poly aspartic acid (being called for short PASP) is a kind of water-soluble polypeptide, the polypeptide of the fertilizer that is often used in agriculture at present, and PASP contains carboxyl, rapidly and nutrient ionic bond exchanging with the H+ that crop root is secreted, the process that nutrient ion is absorbed by crops can be completed.PASP improves utilization rate of fertilizer, rehabilitating soil pollutes and improve soil nutrient availability.
At present, though there is the multiple polypeptide raw material for polypeptide fertilizer, its price is generally expensive, causes the costliness of fertilizer price, and the use for common peasant household has very large restriction, can not be widely used.
Summary of the invention
For at present containing polypeptide fertilizer Problems existing, the invention provides a kind of containing peptide material microbial fertilizer.This fertilizer contains the polypeptide active substance of being degraded by animal-plant material, cheap, promotes that the health of living organism is grown up strong and sturdy, significantly promotes crop yield.
Technical scheme of the present invention is as follows:
A microbial fertilizer containing peptide material, is made up of following component: the hydrolytic activity polypeptide of 5-15%, the complex micro organism fungicide of 3-8%, the fertilizer of 40-60%, the inorganic fertilizer of 20-37% by weight percentage.
In the present invention, described hydrolytic activity polypeptide is that animal-plant material hydrolysis obtains.
In the present invention, described animal/vegetable protein raw material is one or more the mixture in hair-keratin, dregs of beans, rapeseed meal.
In the present invention, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by animal/vegetable protein raw material and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add monoammonium sulfate adjust pH to 2-5 after 24-48 hour, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
In the present invention, described complex micro organism fungicide comprises two or more the mixture in subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium).
In the present invention, described fertilizer component contains the stalk that becomes thoroughly decomposed, the mixture of a kind of in the muck become thoroughly decomposed or two kinds.
In the present invention, described inorganic fertilizer component comprises urea calcium superphosphate, potassium sulfate, and magnesium sulfate is or/and zinc sulfate.
Beneficial effect of the present invention: the present invention contains containing peptide material microbial fertilizer the polypeptide active substance obtained by animal/vegetable protein feed degradation, its protein raw materials is easy to get, cheap, significantly reduce the cost of fertilizer, be of value to production estimation widely and then use.The fertilizer efficiency that obviously can increase microbial fertilizer containing peptide material microbial fertilizer of the present invention, promotes that the health of living organism is grown up strong and sturdy, significantly promotes crop yield.
Embodiment
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.Test method described in embodiment, if no special instructions, is ordinary method; Described reagent and material, if no special instructions, all can obtain from commercial channels, or can prepare in conventional manner.
Embodiment 1
A microbial fertilizer containing peptide material, is made up of following component: the hydrolytic activity polypeptide of 7%, the complex micro organism fungicide of 5%, the fertilizer of 55%, the inorganic fertilizer of 33% by weight percentage.
The preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add monoammonium sulfate adjust pH to 2-5 after 24 hours, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
The mixture that described complex micro organism fungicide comprises subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium) mix by the mass ratio of 1:2:1:2.
Described fertilizer is the stalk that becomes thoroughly decomposed, the mixture that the muck become thoroughly decomposed mixes by the mass ratio of 1:1.
Described inorganic fertilizer component is urea 10%, calcium superphosphate 20%, potassium sulfate 20%, and magnesium sulfate is or/and zinc sulfate 50%.
Embodiment 2
A microbial fertilizer containing peptide material, is made up of following component: the hydrolytic activity polypeptide of 10%, the complex micro organism fungicide of 5%, the fertilizer of 55%, the inorganic fertilizer of 30% by weight percentage.
The preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by dregs of beans and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add monoammonium sulfate adjust pH to 2-5 after 48 hours, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
Described complex micro organism fungicide comprises subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium) by the mixture of 1:1:1 mass ratio.
Described fertilizer is the stalk that becomes thoroughly decomposed, the mixture that the muck become thoroughly decomposed mixes by the mass ratio of 1:2.
Described inorganic fertilizer is urea 10%, calcium superphosphate 20%, potassium sulfate 20%, and magnesium sulfate is or/and zinc sulfate 50%.
Embodiment 3
A microbial fertilizer containing peptide material, is made up of following component: the hydrolytic activity polypeptide of 15%, the complex micro organism fungicide of 8%, the stalk that becomes thoroughly decomposed of 60%, the inorganic fertilizer of 13% by weight percentage.
The preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by hair-keratin and the mixing of 95% aqueous sulfuric acid, at 80 DEG C, be hydrolyzed and add monoammonium sulfate adjust pH to 2-5 after 48 hours, filtration obtains filtrate, and filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
Described complex micro organism fungicide comprises subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum) by the mixture of 1:1:1 mass ratio.
Described inorganic fertilizer component is urea 10%, calcium superphosphate 20%, potassium sulfate 20%, and magnesium sulfate is or/and zinc sulfate 50%.

Claims (7)

1. the microbial fertilizer containing peptide material, is characterized in that, be made up of by weight percentage following component: the hydrolytic activity polypeptide of 5-15%, the complex micro organism fungicide of 3-8%, the fertilizer of 40-60%, the inorganic fertilizer of 20-37%.
2. the microbial fertilizer containing peptide material according to claim 1, is characterized in that, described hydrolytic activity polypeptide is that animal-plant material hydrolysis obtains.
3. the microbial fertilizer containing peptide material according to claim 2, it is characterized in that, described animal/vegetable protein raw material is one or more the mixture in hair-keratin, dregs of beans, rapeseed meal.
4. the microbial fertilizer containing peptide material according to right 3, it is characterized in that, the preparation method of described hydrolytic activity polypeptide is: 1:2 in mass ratio, by animal/vegetable protein raw material and the mixing of 95% aqueous sulfuric acid, at 80-90 DEG C, be hydrolyzed and add monoammonium sulfate adjust pH to 2-5 after 24-48 hour, filter and obtain filtrate, filtrate concentrates postlyophilization, obtains pulverous hydrolytic activity polypeptide.
5. the microbial fertilizer containing peptide material according to claim 1, it is characterized in that, described complex micro organism fungicide comprises two or more the mixture in subtilis (Bacillus subtilis), bacillus licheniformis (Bacillus licheniformis), trichoderma harziarum (Trichoderma harzianum), phosphorus-solubilizing bacteria bacillus megaterium (Bacillus megaterium).
6. the microbial fertilizer containing peptide material according to claim 1, it is characterized in that, described fertilizer component contains the stalk that becomes thoroughly decomposed, the mixture of a kind of in the muck become thoroughly decomposed or two kinds.
7. the microbial fertilizer containing peptide material according to claim 1, it is characterized in that, described inorganic fertilizer component comprises urea, calcium superphosphate, potassium sulfate, and magnesium sulfate is or/and zinc sulfate.
CN201310638649.1A 2013-11-29 2013-11-29 Polypeptide-substance-containing microbial fertilizer Pending CN104671962A (en)

Priority Applications (1)

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CN201310638649.1A CN104671962A (en) 2013-11-29 2013-11-29 Polypeptide-substance-containing microbial fertilizer

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Application Number Priority Date Filing Date Title
CN201310638649.1A CN104671962A (en) 2013-11-29 2013-11-29 Polypeptide-substance-containing microbial fertilizer

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CN104671962A true CN104671962A (en) 2015-06-03

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106631176A (en) * 2016-11-29 2017-05-10 湖北大学 Preparation method and application of slow-release physiological acid salt-polypeptide sulfate for plants
CN107188675A (en) * 2017-06-01 2017-09-22 兰溪市顺光园艺技术有限公司 A kind of flowers chemical fertilizer and preparation method thereof
CN108383668A (en) * 2018-05-25 2018-08-10 合肥市风达农业有限责任公司 A kind of solanaceous vegetables dedicated fertilizer and preparation method thereof
CN111657300A (en) * 2020-06-30 2020-09-15 安徽小溪生态科技发展有限公司 Resource utilization method of livestock and poultry manure and straw and product

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106631176A (en) * 2016-11-29 2017-05-10 湖北大学 Preparation method and application of slow-release physiological acid salt-polypeptide sulfate for plants
CN107188675A (en) * 2017-06-01 2017-09-22 兰溪市顺光园艺技术有限公司 A kind of flowers chemical fertilizer and preparation method thereof
CN108383668A (en) * 2018-05-25 2018-08-10 合肥市风达农业有限责任公司 A kind of solanaceous vegetables dedicated fertilizer and preparation method thereof
CN111657300A (en) * 2020-06-30 2020-09-15 安徽小溪生态科技发展有限公司 Resource utilization method of livestock and poultry manure and straw and product

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Application publication date: 20150603