CN104619848B - 细胞转染方法 - Google Patents

细胞转染方法 Download PDF

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Publication number
CN104619848B
CN104619848B CN201380031734.XA CN201380031734A CN104619848B CN 104619848 B CN104619848 B CN 104619848B CN 201380031734 A CN201380031734 A CN 201380031734A CN 104619848 B CN104619848 B CN 104619848B
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avian
embryos
cells
genetically modified
polynucleotide
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CN104619848A (zh
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S·G·蒂亚克
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Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
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Mat Malta Advanced Technologies Ltd
Commonwealth Scientific and Industrial Research Organization CSIRO
Aviagen Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N2800/90—Vectors containing a transposable element

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CN201380031734.XA 2012-04-20 2013-04-19 细胞转染方法 Active CN104619848B (zh)

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US201261636331P 2012-04-20 2012-04-20
US61/636,331 2012-04-20
US201361783823P 2013-03-14 2013-03-14
US61/783,823 2013-03-14
AU2013204327 2013-04-12
AU2013204327A AU2013204327B2 (en) 2012-04-20 2013-04-12 Cell transfection method
PCT/AU2013/000414 WO2013155572A1 (en) 2012-04-20 2013-04-19 Cell transfection method

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CN (2) CN104619848B (https=)
AU (2) AU2013204327B2 (https=)
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Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
AU2013204327B2 (en) 2012-04-20 2016-09-01 Aviagen Cell transfection method
EP3241902B1 (en) 2012-05-25 2018-02-28 The Regents of The University of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
CN105101951B (zh) * 2012-10-29 2021-08-03 新加坡科技研究局 一种用于基因-药物治疗的新型试剂
EP3363902B1 (en) 2012-12-06 2019-11-27 Sigma Aldrich Co. LLC Crispr-based genome modification and regulation
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
DK2931897T3 (en) 2012-12-12 2018-02-05 Broad Inst Inc CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
MX374090B (es) 2013-03-14 2025-03-05 Caribou Biosciences Inc Composiciones y métodos de ácidos nucleicos dirigidos al ácido nucleico.
KR20250012194A (ko) 2013-06-17 2025-01-23 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용
MX2015017312A (es) 2013-06-17 2017-04-10 Broad Inst Inc Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas.
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
EP3725885A1 (en) 2013-06-17 2020-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
EP3011030B1 (en) 2013-06-17 2023-11-08 The Broad Institute, Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物
BR112016013207A2 (pt) 2013-12-12 2017-09-26 Massachusetts Inst Technology administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
EP3470089A1 (en) * 2013-12-12 2019-04-17 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
MX2016007325A (es) 2013-12-12 2017-07-19 Broad Inst Inc Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos.
AU2014361781B2 (en) 2013-12-12 2021-04-01 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing
CN103782953A (zh) * 2014-01-23 2014-05-14 固镇县玉鹏蔬菜开发有限责任公司 一种小鹅瘟的预防方法
CN105265383A (zh) * 2014-10-18 2016-01-27 颍上县永祥旱粮研究所 一种林下鸡养殖方法
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016106244A1 (en) 2014-12-24 2016-06-30 The Broad Institute Inc. Crispr having or associated with destabilization domains
CN104789599A (zh) * 2015-04-13 2015-07-22 浙江省农业科学院 一种提高慢病毒胚盘下腔注射制备转基因家禽效率的方法
TWI813532B (zh) 2015-06-18 2023-09-01 美商博得學院股份有限公司 降低脱靶效應的ｃｒｉｓｐｒ酶突變
WO2016205759A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
WO2017024343A1 (en) * 2015-08-07 2017-02-16 Commonwealth Scientific And Industrial Research Organisation Method for producing an animal comprising a germline genetic modification
EP3380611A4 (en) 2015-11-24 2020-02-12 Commonwealth Scientific and Industrial Research Organisation PRODUCTION OF VIRUSES IN A CELL CULTURE
BR112018010503A2 (pt) 2015-11-24 2018-11-13 Commw Scient Ind Res Org ovo de ave, método para replicar um vírus, vírus, método para produzir uma composição de vacina, composição de vacina, ave transgênica, e método para produzir uma ave
WO2017111144A1 (ja) * 2015-12-25 2017-06-29 国立研究開発法人産業技術総合研究所 遺伝子改変家禽卵
EP3629719B1 (en) * 2017-05-25 2023-08-23 Eggxyt Ltd Methods for gender determination of avian embryos in unhatched eggs and means thereof
CA3065317A1 (en) 2017-05-31 2018-12-06 Commonwealth Scientific And Industrial Research Organisation Trait selection in avians
CN109275622A (zh) * 2017-07-20 2019-01-29 龚海华 一种蛋鸡的循环养殖方法
CN107723313A (zh) * 2017-10-30 2018-02-23 扬州大学 一种外源物质导入鸡胚的方法
MX2020004811A (es) * 2017-11-15 2020-10-07 Weird Science Llc Métodos y composiciones de reconstrucción no mieloablativa de médula ósea.
KR102103103B1 (ko) * 2018-08-16 2020-04-21 (주)라트바이오 인위적 뉴클레아제를 생산하는 형질전환 동물 및 형질전환 배아
EP3953480B1 (en) 2019-04-08 2025-12-03 Dna Twopointo Inc. Transposition of nucleic acids into eukaryotic genomes with a transposase from heliothis
EP3953481A4 (en) * 2019-04-08 2023-01-25 Dna Twopointo Inc. INTEGRATION OF NUCLEIC ACID CONSTRUCTS INTO EUKARYOTIC CELLS USING A TRANSPOSASE FROM ORYZIAS
WO2025226643A1 (en) * 2024-04-23 2025-10-30 TransCytos, LLC Devices and methods for transfection
CN118575784B (zh) * 2024-06-27 2025-09-02 江苏省家禽科学研究所 一种提高肉鸡肌肉营养价值的养殖方法

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5162215A (en) * 1988-09-22 1992-11-10 Amgen Inc. Method of gene transfer into chickens and other avian species
GB8906214D0 (en) 1989-03-17 1989-05-04 Nat Res Dev Introducing an exogenous gene into birds
US5631329A (en) 1990-08-27 1997-05-20 Dendritech, Inc. Process for producing hyper-comb-branched polymers
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5266106A (en) 1992-10-22 1993-11-30 Xerox Corporation Ink compositions with dendrimer grafts
WO1996031549A1 (de) 1995-04-07 1996-10-10 Mueller Egbert Dendrimere pfropfpolymerisate
US20030069173A1 (en) 1998-03-16 2003-04-10 Life Technologies, Inc. Peptide-enhanced transfections
JP4320054B2 (ja) 1996-08-13 2009-08-26 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド ポリヌクレオチド送達のための組成物および方法
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
AU736087B2 (en) 1997-08-04 2001-07-26 University Of Massachusetts Avian primordial germ cell (PGC) cell line and a method for long term culturing thereof
EP1019491B1 (en) 1997-08-04 2005-12-07 University of Massachusetts, a Public Institution of Higher Education of The Commonwealth of Massachusetts, Production of avian embryonic germ (eg) cell lines by prolonged culturing of pgcs, use thereof for cloning and chimerization
DK1023442T3 (da) * 1997-10-16 2011-03-14 Univ Georgia Transgenetiske fugle og fremstilling af proteiner
ATE378398T1 (de) 1999-02-11 2007-11-15 Hanmi Pharm Ind Co Ltd Pluripotente embryonale keimzellinie aus vögeln
US6140118A (en) 1999-08-11 2000-10-31 Wisconsin Alumni Research Foundation Avian blastodermal cell lines
JP4609869B2 (ja) 1999-12-03 2011-01-12 独立行政法人科学技術振興機構 トランスポゾン転移酵素および遺伝子改変方法
US8202979B2 (en) 2002-02-20 2012-06-19 Sirna Therapeutics, Inc. RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid
JP4300287B2 (ja) 2002-02-14 2009-07-22 国立大学法人東京海洋大学 分離始原生殖細胞の移植による生殖細胞系列への分化誘導法
JP4364474B2 (ja) 2002-02-15 2009-11-18 大学共同利用機関法人情報・システム研究機構 哺乳動物において機能的なトランスポゾン
US20040172667A1 (en) * 2002-06-26 2004-09-02 Cooper Richard K. Administration of transposon-based vectors to reproductive organs
US7527966B2 (en) * 2002-06-26 2009-05-05 Transgenrx, Inc. Gene regulation in transgenic animals using a transposon-based vector
KR100502889B1 (ko) 2003-04-21 2005-07-20 한미약품(주) 조류 원시생식세포의 생식선 전이 효율의 개선방법
US20060174362A1 (en) * 2005-02-01 2006-08-03 Origen Therapeutics, Inc. Long-term culture of avian primordial germ cells (PGCs)
WO2006084035A2 (en) 2005-02-01 2006-08-10 Origen Therapeutics, Inc. Transgenic chickens
WO2007017759A2 (en) * 2005-03-31 2007-02-15 Ronen Kahana Creating poultry and other animals resistant to viral disease
US20070293449A1 (en) 2006-06-20 2007-12-20 Nastech Pharmaceutical Company Inc. Compositions and methods for delivery of double-stranded rna
US9217026B2 (en) * 2006-11-13 2015-12-22 Sangamo Biosciences, Inc. Method of inactivating a glucocorticoid receptor gene in an isolated cell
MX2009012317A (es) * 2007-05-16 2010-04-01 Mat Malta Advanced Technologie Tratamiento y prevencion de influenza.
JP5554231B2 (ja) * 2007-06-13 2014-07-23 コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション トリの生産形質の改変
WO2009023800A1 (en) * 2007-08-14 2009-02-19 Origen Therapeutics Transgenic chickens with an inactivated endogenous gene locus
NZ583783A (en) * 2007-09-20 2012-10-26 Commw Scient Ind Res Org Avian interferon-lambda polypeptide and genetic sequences encoding the same
KR102262704B1 (ko) * 2009-08-11 2021-06-09 상가모 테라퓨틱스, 인코포레이티드 표적화 변형에 대한 동형접합성 유기체
BR112013021785A8 (pt) * 2011-02-25 2018-07-03 Recombinetics Inc animais geneticamente modificados e métodos para fazer os mesmos
AU2013204327B2 (en) 2012-04-20 2016-09-01 Aviagen Cell transfection method
WO2014138792A1 (en) * 2013-03-14 2014-09-18 Commonwealth Scientific And Industrial Research Organisation Double-stranded rna
JP2016522679A (ja) * 2013-04-04 2016-08-04 プレジデント アンド フェローズ オブ ハーバード カレッジ ＣＲＩＳＰＲ／Ｃａｓ系を用いたゲノム編集の治療的使用
GB201310031D0 (en) * 2013-06-05 2013-07-17 Pirbright Inst The Cell
EP3169778B1 (en) * 2014-07-14 2023-10-25 Washington State University Nanos knock-out that ablates germline cells
WO2017024343A1 (en) * 2015-08-07 2017-02-16 Commonwealth Scientific And Industrial Research Organisation Method for producing an animal comprising a germline genetic modification
BR112018010503A2 (pt) * 2015-11-24 2018-11-13 Commw Scient Ind Res Org ovo de ave, método para replicar um vírus, vírus, método para produzir uma composição de vacina, composição de vacina, ave transgênica, e método para produzir uma ave
CN105524941A (zh) * 2016-01-19 2016-04-27 扬州大学 一种特异于禽类原始生殖细胞的基因转移系统及方法
TW202241259A (zh) * 2020-12-31 2022-11-01 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） 不育禽類胚胎、其產生及用途

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
" Suppression of avian influenza transmission in genetically modified chicken";LYALL，J.et al.;《Science》;20110114;第331卷(第6014期);摘要 *
"Gene Transfer into Circulating Primordial Germ Cells of Quail Embryos";ONO，T.et al.;《Experimental Animals》;19951231;第44卷(第4期);摘要，第276页左栏第一段，第278页左栏 *
"Production of Biofunctional Recombinant Human Interleukin 1 Receptor Antagonist (rhIL1RN) from Transgenic Quail Egg White";KWON，S.C.et al.;《BIOLOGY OF REPRODUCTION》;20100210;第82卷(第6期);摘要 *
"Transgenic chimera quail production by microinjecting lentiviral vector into the blood vessel of the early embryo";SUN，P.et al.;《Animal Science Journal》;20120430;第83卷(第4期);摘要 *

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