CN104541667B - A kind of method promoting Desmodium styracifolium seed germination - Google Patents
A kind of method promoting Desmodium styracifolium seed germination Download PDFInfo
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- CN104541667B CN104541667B CN201510053982.5A CN201510053982A CN104541667B CN 104541667 B CN104541667 B CN 104541667B CN 201510053982 A CN201510053982 A CN 201510053982A CN 104541667 B CN104541667 B CN 104541667B
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Abstract
The present invention provides a kind of method promoting Desmodium styracifolium seed germination, comprises the following steps: step one, with poly-L alcoholic solution soak Desmodium styracifolium seed；Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, cultivate under the conditions of constant temperature alternate illumination, and described constant temperature alternate illumination condition is: cultivate under 30 DEG C of dark conditions every day 12 hours, then cultivate 12 hours under 30 DEG C of illumination conditions.The present invention has effectively broken the strong dormancy of Desmodium styracifolium seed, low cost, non-environmental-pollution, without dangerous, simple to operate, improve germination percentage and the germination regularity of Desmodium styracifolium seed, the germinating energy of Desmodium styracifolium I level seed is brought up to 82 89%, germination percentage has brought up to 89 96%.
Description
Technical field
The present invention relates to plant seed germination technical field.It is more particularly related to tool one promotees
The method entering Desmodium styracifolium seed germination.
Background technology
Desmodium styracifolium is pulse family beggar-ticks upright suffruticose draft, and aerial part is used as medicine, and is two broadly
One of district's large class Chinese medicine, has dampness removing removing jaundice, effect of inducing diuresis for treating strangurtia, red for jaundice urine, heat
Drench, urolithiasis, difficulty and pain in micturition, the treatment of the diseases such as oedema oliguria.Owing to its medicinal efficacy is notable, medicine city
The demand of Desmodium styracifolium medicinal material is constantly increased by field, and wild resource cannot meet the market demand, manually
Cultivation Desmodium styracifolium not only protects wild resource, has preferable economic benefit and social benefit simultaneously.
Artificial cultivation Desmodium styracifolium is typically seminal propagation, but due to the Desmodium styracifolium strong existence of seed seed coat
Strong dormancy, thus cause that emergence rate is low, it is irregular to emerge, then affect plant strain growth.With
Time, the fruit phase of Desmodium styracifolium is longer, and seed quality and the strong degree of Different Harvesting Time all exist significance difference
Different, seed quality is uneven emerges when being also and cause artificial cultivation uneven major reason.
At present, production releases the seed dormant method of Desmodium styracifolium and be mainly concentrated sulfuric acid corrosion seed coat or machine
Tool friction scratches seed coat.The concentrated sulfuric acid has severe corrosive, and operation has certain danger；Etching time is relatively
Difficult assurance, etching time length easily causes acid evil；Concentrated sulfuric acid price is high, difficult recovery, easily causes environmental pollution.
The method operating force of mechanical friction is difficult to uniformly arrive every seed, easily causes seed and damages completely or have no
The phenomenon of damage.Therefore, need badly a kind of low cost, non-environmental-pollution, without side dangerous, simple to operate
Method releases Desmodium styracifolium seed dormancy, improves germination percentage and germination regularity.
Summary of the invention
It is an object of the invention to solve at least the above and/or defect, and provide after at least and will say
Bright advantage.
A further object of the invention is by a kind of method promoting Desmodium styracifolium seed germination, and it is one
Kind of low cost, non-environmental-pollution, without the seed dormant method of releasing Desmodium styracifolium dangerous, simple to operate,
Improve germination percentage and the germination regularity of Desmodium styracifolium seed.
In order to realize according to object of the present invention and further advantage, it is provided that a kind of promotion Desmodium styracifolium
The method of seed germination, comprises the following steps:
Step one, with polyglycol solution soak Desmodium styracifolium seed；
Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, in constant temperature alternate illumination
Under the conditions of cultivate, described constant temperature alternate illumination condition is: it is little that every day cultivates 12 under 30 DEG C of dark conditions
Time, then cultivate 12 hours under 30 DEG C of illumination conditions.
Preferably, the described method promoting Desmodium styracifolium seed germination, before described step one first
Desmodium styracifolium seed is divided into three grades, then by I grade, II grade and III grade of seed with mass fraction is respectively
20%-30% polyglycol solution soaks 44-48 hour, 36-40 hour and 20-24 hour, immersion process
Middle holding solution temperature is 20-30 DEG C；
Wherein, I grade of seed, long 1.8-2.3mm, wide 1.2-1.7mm, mass of 1000 kernel is more than 1.80 grams；Ⅱ
Level seed, long 1.5-2.3mm, wide 1.0-1.7mm, mass of 1000 kernel is 1.65-1.85 gram；III grade of seed, long
1.2-2.3mm, wide 0.8-1.7mm, mass of 1000 kernel is less than 1.65 grams.
Preferably, the described method promoting Desmodium styracifolium seed germination, after described step one,
Also include: Desmodium styracifolium seed is put into the liquor natrii hypochloritis that mass fraction is 1% and soaks 15-30 minute,
Then distilled water flushing is used 3-5 time.
Preferably, the described method promoting Desmodium styracifolium seed germination, in described step one, extensively gold
The degree of depth that money grass seed is immersed in polyglycol solution at least 5cm.
Preferably, the described method promoting Desmodium styracifolium seed germination, described culture medium is agar matter
Amount mark is the solid medium of 1%.
Preferably, the described method promoting Desmodium styracifolium seed germination, after described step one, also
Including:
Step a, Desmodium styracifolium seed is placed in glass container, and allows Desmodium styracifolium seed hold at glass
Forming individual layer in device, adding concentration is the salt solution of 10%-15%, and salt water depth is 1-1.5mm；
Step b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is
220 volts, pulse frequency be 400-500MHz, shelve 20-30 minute；
Step c, by step b in triplicate.
Preferably, the described method promoting Desmodium styracifolium seed germination, after described step one, also
Including:
The Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then presses 1:3
Weight ratio Desmodium styracifolium seed is mixed with vermiculite, add mass fraction be the Gibberellins solution of 0.05%,
24-36 hour is stood in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, with mass fraction be
The abscisic acid solution of 0.4% soaks 24h, is mixed with vermiculite by Desmodium styracifolium seed by the weight ratio of 1:3, adds
Add the abscisic acid solution that mass fraction is 0.4%, in the thermostatic chamber that temperature is 20 DEG C, stand 24-36 hour；
Sift out Desmodium styracifolium seed, soak 24h by the Gibberellins solution that mass fraction is 0.2%, by the weight of 1:3
Amount ratio mixes with vermiculite, and adding mass fraction is the Gibberellins solution of 0.2%, at the constant temperature that temperature is 25 DEG C
Indoor standing 36-48 hour.
Preferably, the described method promoting Desmodium styracifolium seed germination, the average grain diameter of described vermiculite
For 2-4 millimeter, water content is 30%-40%.
Preferably, the described method promoting Desmodium styracifolium seed germination, the intensity of described illumination is
1500-2000Lx。
The present invention at least includes following beneficial effect:
(1) present invention has effectively broken the strong dormancy of Desmodium styracifolium seed, low cost, non-environmental-pollution,
Without dangerous, simple to operate, improve germination percentage and the germination regularity of Desmodium styracifolium seed, by wide money
The germinating energy of I grade of seed of grass brings up to 82-89%, and germination percentage has brought up to 89-96%.
(2) Desmodium styracifolium seed is divided into I grade, II grade and III grade by the present invention, soaks the most respectively
Process, decrease the time of invalid immersion, and avoid excessive imbibition, cause seed to damage, affect seed
Germinate.
(3) step a, Desmodium styracifolium seed is placed in glass container, and allow Desmodium styracifolium seed at glass
Forming individual layer in glass container, adding concentration is the salt solution of 10%-15%, and salt water depth is 1-1.5mm；Step
Rapid b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is 220
Volt, pulse frequency are 400-500MHz, shelve 20-30 minute；Step c, by step b in triplicate.
The sucting expansion seed electric shock obtained through immersion is carried out optimal stimulation, it is possible to help to break Desmodium styracifolium kind
The dormancy of son, stimulates cell division, improves the metabolic speed of seed, wide money after shocking by electricity
The germination percentage ratio of grass seed does not carry out the high 10-13% that shocks by electricity.
(4) Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then
Being mixed with vermiculite by Desmodium styracifolium seed by the weight ratio of 1:3, adding mass fraction is the gibberellin of 0.05%
Solution, stands 24-36 hour in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, use quality
Mark is the abscisic acid solution immersion 24h of 0.4%, by the weight ratio of 1:3 by Desmodium styracifolium seed and vermiculite
Mixing, adds the abscisic acid solution that mass fraction is 0.4%, stands in the thermostatic chamber that temperature is 20 DEG C
24-36 hour；Sift out Desmodium styracifolium seed, soak 24h by the Gibberellins solution that mass fraction is 0.2%,
Mixing with vermiculite by the weight ratio of 1:3, adding mass fraction is the Gibberellins solution of 0.2%, in temperature is
36-48 hour is stood in the thermostatic chamber of 25 DEG C.First seed dormancy is suppressed with the gibberellin of low concentration,
Promote germination, then improve the cold hardiness of plant, drought resistance and Salt-endurance with abscisic acid, but
Abscisic acid has certain seed dormant effect that makes, and the most again promotes cell with high concentration gibberellin
Division, thus accelerate seed germination.Vermiculite has good heat insulating ability, gas permeability and moisture retention, uses
Vermiculite, as matrix, then adds gibberellin in vermiculite and abscisic acid is strengthened gibberellin further and comes off
The action effect of acid, it is to avoid temperature, humidity is uneven and gas permeability causes the desmodium seed can not
The degree of breaking dormancy or breaking dormancy is uneven.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will
By the research of the present invention and practice are understood by the person skilled in the art.
Detailed description of the invention
Below in conjunction with example, the present invention is described in further detail, to make those skilled in the art's reference
Specification word can be implemented according to this.
Should be appreciated that used herein such as " have ", " comprising " and " including " term not
Allot other element one or more or the existence of a combination thereof or interpolation.
<example 1>
A kind of method promoting Desmodium styracifolium seed germination, comprises the following steps:
Step one, with polyglycol solution soak Desmodium styracifolium seed；
Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, in constant temperature alternate illumination
Under the conditions of cultivate, described constant temperature alternate illumination condition is: it is little that every day cultivates 12 under 30 DEG C of dark conditions
Time, then cultivate 12 hours under 30 DEG C of illumination conditions.
The described method promoting Desmodium styracifolium seed germination, first by Desmodium styracifolium before described step one
Seed is divided into three grades, is then 20% poly-second two with mass fraction respectively by I grade, II grade and III grade of seed
Alcoholic solution soaks 44 hours, 36 hours and 20 hours, and keeping solution temperature in immersion process is 20 DEG C；
Wherein, I grade of seed, long 1.8-2.3mm, wide 1.2-1.7mm, mass of 1000 kernel is more than 1.80 grams；Ⅱ
Level seed, long 1.5-2.3mm, wide 1.0-1.7mm, mass of 1000 kernel is 1.65-1.85 gram；III grade of seed, long
1.2-2.3mm, wide 0.8-1.7mm, mass of 1000 kernel is less than 1.65 grams.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes: will
Desmodium styracifolium seed is put into the liquor natrii hypochloritis that mass fraction is 1% and is soaked 15 minutes, then with distillation
Water rinses 3 times.
The described method promoting Desmodium styracifolium seed germination, in described step one, Desmodium styracifolium seed soaks
The degree of depth in polyglycol solution is not 5cm.
The described method promoting Desmodium styracifolium seed germination, described culture medium be agar mass fraction be 1%
Solid medium.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
Step a, Desmodium styracifolium seed is placed in glass container, and allows Desmodium styracifolium seed at glass container
Middle formation individual layer, adding concentration is the salt solution of 10%, and salt water depth is 1-1.5mm；
Step b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is
220 volts, pulse frequency be 400MHz, shelve 20 minutes；
Step c, by step b in triplicate.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
The Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then presses 1:3
Weight ratio Desmodium styracifolium seed is mixed with vermiculite, add mass fraction be the Gibberellins solution of 0.05%,
24 hours are stood in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, be 0.4% with mass fraction
Abscisic acid solution soak 24h, by the weight ratio of 1:3, Desmodium styracifolium seed is mixed with vermiculite, interpolation matter
Amount mark is the abscisic acid solution of 0.4%, stands 24 hours in the thermostatic chamber that temperature is 20 DEG C；Sift out
Desmodium styracifolium seed, soaks 24h by the Gibberellins solution that mass fraction is 0.2%, by the weight ratio of 1:3
Mixing with vermiculite, adding mass fraction is the Gibberellins solution of 0.2%, in the thermostatic chamber that temperature is 25 DEG C
Stand 36 hours.
The described method promoting Desmodium styracifolium seed germination, the average grain diameter of described vermiculite is 2-4 millimeter,
Water content is 30%.
The described method promoting Desmodium styracifolium seed germination, the intensity of described illumination is 1500Lx.
<example 2>
A kind of method promoting Desmodium styracifolium seed germination, comprises the following steps:
Step one, with polyglycol solution soak Desmodium styracifolium seed；
Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, in constant temperature alternate illumination
Under the conditions of cultivate, described constant temperature alternate illumination condition is: it is little that every day cultivates 12 under 30 DEG C of dark conditions
Time, then cultivate 12 hours under 30 DEG C of illumination conditions.
The described method promoting Desmodium styracifolium seed germination, first by Desmodium styracifolium before described step one
Seed is divided into three grades, is then that 20%-30% gathers with mass fraction respectively by I grade, II grade and III grade of seed
Ethylene glycol solution soaks 48 hours, 40 hours and 24 hours, and in immersion process, holding solution temperature is
30℃；
Wherein, I grade of seed, long 1.8-2.3mm, wide 1.2-1.7mm, mass of 1000 kernel is more than 1.80 grams；Ⅱ
Level seed, long 1.5-2.3mm, wide 1.0-1.7mm, mass of 1000 kernel is 1.65-1.85 gram；III grade of seed, long
1.2-2.3mm, wide 0.8-1.7mm, mass of 1000 kernel is less than 1.65 grams.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes: will
Desmodium styracifolium seed is put into the liquor natrii hypochloritis that mass fraction is 1% and is soaked 30 minutes, then with distillation
Water rinses 5 times.
The described method promoting Desmodium styracifolium seed germination, in described step one, Desmodium styracifolium seed soaks
The not degree of depth position 10cm in polyglycol solution.
The described method promoting Desmodium styracifolium seed germination, described culture medium be agar mass fraction be 1%
Solid medium.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
Step a, Desmodium styracifolium seed is placed in glass container, and allows Desmodium styracifolium seed at glass container
Middle formation individual layer, adding concentration is the salt solution of 15%, and salt water depth is 1-1.5mm；
Step b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is
220 volts, pulse frequency be 500MHz, shelve 30 minutes；
Step c, by step b in triplicate.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
The Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then presses 1:3
Weight ratio Desmodium styracifolium seed is mixed with vermiculite, add mass fraction be the Gibberellins solution of 0.05%,
36 hours are stood in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, be 0.4% with mass fraction
Abscisic acid solution soak 24h, by the weight ratio of 1:3, Desmodium styracifolium seed is mixed with vermiculite, interpolation matter
Amount mark is the abscisic acid solution of 0.4%, stands 36 hours in the thermostatic chamber that temperature is 20 DEG C；Sift out
Desmodium styracifolium seed, soaks 24h by the Gibberellins solution that mass fraction is 0.2%, by the weight ratio of 1:3
Mixing with vermiculite, adding mass fraction is the Gibberellins solution of 0.2%, in the thermostatic chamber that temperature is 25 DEG C
Stand 48 hours.
The described method promoting Desmodium styracifolium seed germination, the average grain diameter of described vermiculite is 2-4 millimeter,
Water content is 40%.
The described method promoting Desmodium styracifolium seed germination, the intensity of described illumination is 2000Lx.
<example 3>
A kind of method promoting Desmodium styracifolium seed germination, comprises the following steps:
Step one, with polyglycol solution soak Desmodium styracifolium seed；
Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, in constant temperature alternate illumination
Under the conditions of cultivate, described constant temperature alternate illumination condition is: it is little that every day cultivates 12 under 30 DEG C of dark conditions
Time, then cultivate 12 hours under 30 DEG C of illumination conditions.
The described method promoting Desmodium styracifolium seed germination, first by Desmodium styracifolium before described step one
Seed is divided into three grades, is then that 20%-30% gathers with mass fraction respectively by I grade, II grade and III grade of seed
Ethylene glycol solution soaks 45 hours, 38 hours and 22 hours, and in immersion process, holding solution temperature is
25℃；
Wherein, I grade of seed, long 1.8-2.3mm, wide 1.2-1.7mm, mass of 1000 kernel is more than 1.80 grams；Ⅱ
Level seed, long 1.5-2.3mm, wide 1.0-1.7mm, mass of 1000 kernel is 1.65-1.85 gram；III grade of seed, long
1.2-2.3mm, wide 0.8-1.7mm, mass of 1000 kernel is less than 1.65 grams.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes: will
Desmodium styracifolium seed is put into the liquor natrii hypochloritis that mass fraction is 1% and is soaked 20 minutes, then with distillation
Water rinses 4 times.
The described method promoting Desmodium styracifolium seed germination, in described step one, Desmodium styracifolium seed soaks
The degree of depth in polyglycol solution is not 8cm.
The described method promoting Desmodium styracifolium seed germination, described culture medium be agar mass fraction be 1%
Solid medium.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
Step a, Desmodium styracifolium seed is placed in glass container, and allows Desmodium styracifolium seed at glass container
Middle formation individual layer, adding concentration is the salt solution of 12%, and salt water depth is 1-1.5mm；
Step b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is
220 volts, pulse frequency be 450MHz, shelve 22 minutes；
Step c, by step b in triplicate.
The described method promoting Desmodium styracifolium seed germination, after described step one, also includes:
The Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then presses 1:3
Weight ratio Desmodium styracifolium seed is mixed with vermiculite, add mass fraction be the Gibberellins solution of 0.05%,
30 hours are stood in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, be 0.4% with mass fraction
Abscisic acid solution soak 24h, by the weight ratio of 1:3, Desmodium styracifolium seed is mixed with vermiculite, interpolation matter
Amount mark is the abscisic acid solution of 0.4%, stands 30 hours in the thermostatic chamber that temperature is 20 DEG C；Sift out
Desmodium styracifolium seed, soaks 24h by the Gibberellins solution that mass fraction is 0.2%, by the weight ratio of 1:3
Mixing with vermiculite, adding mass fraction is the Gibberellins solution of 0.2%, in the thermostatic chamber that temperature is 25 DEG C
Stand 40 hours.
The described method promoting Desmodium styracifolium seed germination, the average grain diameter of described vermiculite is 2-4 millimeter,
Water content is 35%.
The described method promoting Desmodium styracifolium seed germination, the intensity of described illumination is 1800Lx.
In order to the effect of the present invention is described, inventor provides comparative experiments as follows:
<comparative example 1>
In stepb, Desmodium styracifolium seed is not shocked by electricity, complete with example 3 of remaining parameter
Identical, technical process is the most identical.
<comparative example 2>
With after gibberellin or desulfurization acid soak Desmodium styracifolium seed, Desmodium styracifolium seed is not mixed with vermiculite
Close storage.Remaining parameter is identical with example 3, and technical process is the most identical.
[each example of table 1 and the germination percentage of each comparative example and germinating energy]
| Germination percentage | Germinating energy | |
| Example 1 | 89% | 82% |
| Example 2 | 91% | 85% |
| Example 3 | 96% | 89% |
| Comparative example 1 | 84% | 85% |
| Comparative example 2 | 88% | 80% |
From upper table 1 it can be seen that in example 3 due to electric shock Desmodium styracifolium seed, its germination percentage and germination
Gesture is significantly higher than the situation not carrying out shocking by electricity in comparative example 1.And in example 3, due to gibberellin
After desulfurization acid soak Desmodium styracifolium seed, then by Desmodium styracifolium seed and vermiculite combined storage, its
Bud rate and germinating energy are also apparently higher than the situation in comparative example 2.
Visible, the present invention uses electric shock Desmodium styracifolium seed and by Desmodium styracifolium seed and vermiculite combined storage
Method all have and improve germinating energy and the effect of germination percentage significantly.
<example 4>
On September 10th, 2014 buys 2 kilograms of 1 batch of Desmodium styracifolium seed from Yulin medicinal material market, experiment
Indoor clean, classification, obtain I grade of seed, 0.528 kilogram, III grade seed of 1.106 kilograms, II grade seed
0.215 kilogram, account for the 55.3% of total seed number, 26.4%, 10.8% respectively.By 500 clean seeds respectively
Being soaked in 150 milliliters of PEG solution, the PEG concentration of seed soaking and seed soaking time refer to table 2.Not soak
The seed planted compares.
Table 2 Desmodium styracifolium seed-soaking processing mode record
Liquor natrii hypochloritis's immersion that the Desmodium styracifolium seed of imbibition after PEG seed soaking puts into concentration 1% is disappeared
Poison 15～30 minutes, with distilled water flushing 3～5 times after sterilization.Control treatment seed is not disinfected.
1% agar that Desmodium styracifolium seed after hypochlorite disinfectant is placed in the culture dish of a diameter of 9cm is sent out
On bud bed, 30 DEG C of constant temperature, alternate illumination that 12 hours illumination/12 hour are dark illumination box in
Cultivate 8 days.50 seeds put by each culture dish, and each process is repeated 4 times, the seed of control treatment
Germination condition is ibid.6th day potentiality of seed, the 8th day survey percentage of seedgermination, statistics after pendulum kind
It is shown in Table 3.From table 3, by the technology of the present invention can be effectively facilitated the sprouting of Desmodium styracifolium seed with
Seedling, and the germinating energy 19% of control treatment, germination percentage 35% compares, sending out of I grade of seed of Desmodium styracifolium
Bud gesture brings up to 82～89%, and germination percentage has brought up to 89～96%.The technology of the present invention is to Desmodium styracifolium seed
Sprouting has significant facilitation.
Table 3 Desmodium styracifolium germination is added up
<example 5>
A kind of method promoting Desmodium styracifolium seed germination, comprises the following steps:
(1) seed cleans classification: graded index includes seed appearance color, cleanliness, mass of 1000 kernel, water content,
I grade, II grade, III grade qualified seed respectively:
I grade of seed seed kidney shape, flat, long 1.8mm～2.3mm, wide 1.2mm～1.7mm, mass of 1000 kernel
＞ 1.80 grams.Surface yellow or light yellow, full glossy.Cleanliness ＞ 95%.Water content 7～10%.
Account for the 60% of seed sum.
II grade of seed seed kidney shape, flat, long 1.5mm～2.3mm, wide 1.0mm～1.7mm, thousand
Weigh 1.65～1.85 grams.Surface dark-brown, brownish red or bronzing, full glossy.Cleanliness 90～95%.
Water content 7～10%.Account for the 30% of seed sum.
III grade of seed seed kidney shape, flat, long 1.2mm～2.3mm, wide 0.8mm～1.7mm, thousand
Weight ＜ 1.65 grams.Surface look green, green and brown, the fullest shrivelled shrinkage.Cleanliness ＞ 90%.Water content 7～
10%.Account for the 10% of seed sum.
(2) PEG seed soaking:
I grade of seed with 20～30% polyethylene glycol (PEG) solution soak clean seed 44～48 hours, water temperature is protected
Hold at 20～30 DEG C, after having soaked seed, again separate the poor seed emerged.
II grade of seed with 20～30% polyethylene glycol (PEG) solution soak clean seed 36～40 hours, water temperature is protected
Hold at 20～30 DEG C, after having soaked seed, again separate the poor seed emerged.
III grade of seed with 20～30% polyethylene glycol (PEG) solution soak clean seed 20～24 hours, water temperature is protected
Hold at 20～30 DEG C, after having soaked seed, again separate the poor seed emerged.
(3) sterilization: after being soaked seed by PEG, the Desmodium styracifolium seed of imbibition puts into the hypochlorous acid that mass fraction is 1%
Sodium solution soaking disinfection 15～30 minutes, with distilled water flushing 3～5 times after sterilization.
(4) germinate: the Desmodium styracifolium seed sterilized is placed on the germinating bed of 1% agar medium and germinates
Cultivating, within the 5th～6 day, seed is sprouted for the first time, can germinate complete to the 8th day.The suitable bar of germination
Part is: temperature is 25～35 DEG C.Illumination is nature light.
Although embodiment of the present invention are disclosed as above, but it is not restricted to specification and embodiment party
Listed utilization in formula.It can be applied to various applicable the field of the invention completely.For being familiar with ability
For the personnel in territory, it is easily achieved other amendment.Therefore without departing substantially from claim and etc. homotype
Enclosing under limited universal, the present invention is not limited to specific details and shown here as the reality with description
Example.
Claims (8)
1. the method promoting Desmodium styracifolium seed germination, it is characterised in that comprise the following steps:
Step one, with polyglycol solution soak Desmodium styracifolium seed；
Step 2, Desmodium styracifolium seed step one obtained are placed in culture medium, in constant temperature alternate illumination
Under the conditions of cultivate, described constant temperature alternate illumination condition is: it is little that every day cultivates 12 under 30 DEG C of dark conditions
Time, then cultivate 12 hours under 30 DEG C of illumination conditions；First by Desmodium styracifolium before described step one
Seed is divided into three grades, is then that 20%-30% gathers with mass fraction respectively by I grade, II grade and III grade of seed
Ethylene glycol solution soaks 44-48 hour, 36-40 hour and 20-24 hour, keeps solution in immersion process
Temperature is 20-30 DEG C；
Wherein, I grade of seed, long 1.8-2.3mm, wide 1.2-1.7mm, mass of 1000 kernel is more than 1.80 grams；Ⅱ
Level seed, long 1.5-2.3mm, wide 1.0-1.7mm, mass of 1000 kernel is 1.65-1.85 gram；III grade of seed, long
1.2-2.3mm, wide 0.8-1.7mm, mass of 1000 kernel is less than 1.65 grams.
2. the method promoting Desmodium styracifolium seed germination as claimed in claim 1, it is characterised in that
After described step one, also include: Desmodium styracifolium seed is put into the sodium hypochlorite that mass fraction is 1% molten
Immersion is steeped 15-30 minute, then uses distilled water flushing 3-5 time.
3. the method promoting Desmodium styracifolium seed germination as claimed in claim 2, it is characterised in that institute
State in step one, the degree of depth that Desmodium styracifolium seed is immersed in polyglycol solution at least 5cm.
4. the method promoting Desmodium styracifolium seed germination as claimed in claim 2, it is characterised in that institute
State culture medium be agar mass fraction be the solid medium of 1%.
5. the method promoting Desmodium styracifolium seed germination as described in arbitrary in claim 1-4, its feature
It is, after described step one, also includes:
Step a, Desmodium styracifolium seed is placed in glass container, and allows Desmodium styracifolium seed at glass container
Middle formation individual layer, adding concentration is the salt solution of 10%-15%, and salt water depth is 1-1.5mm；
Step b, Desmodium styracifolium seed carrying out shock treatment, shock by electricity second time 15-20, shock voltage is
220 volts, pulse frequency be 400-500MHz, shelve 20-30 minute；
Step c, by step b in triplicate.
6. the method promoting Desmodium styracifolium seed germination as described in arbitrary in claim 1-4, its feature
It is, after described step one, also includes:
The Gibberellins solution that Desmodium styracifolium seed mass fraction is 0.05% is soaked 24h, then presses 1:3
Weight ratio Desmodium styracifolium seed is mixed with vermiculite, add mass fraction be the Gibberellins solution of 0.05%,
24-36 hour is stood in the refrigerator that temperature is 2-4 DEG C；Sift out Desmodium styracifolium seed, with mass fraction be
The abscisic acid solution of 0.4% soaks 24h, is mixed with vermiculite by Desmodium styracifolium seed by the weight ratio of 1:3, adds
Add the abscisic acid solution that mass fraction is 0.4%, in the thermostatic chamber that temperature is 20 DEG C, stand 24-36 hour；
Sift out Desmodium styracifolium seed, soak 24h by the Gibberellins solution that mass fraction is 0.2%, by the weight of 1:3
Amount ratio mixes with vermiculite, and adding mass fraction is the Gibberellins solution of 0.2%, at the constant temperature that temperature is 25 DEG C
Indoor standing 36-48 hour.
7. the method promoting Desmodium styracifolium seed germination as claimed in claim 6, it is characterised in that institute
The average grain diameter stating vermiculite is 2-4 millimeter, and water content is 30%-40%.
8. the method promoting Desmodium styracifolium seed germination as claimed in claim 1, it is characterised in that institute
The intensity stating illumination is 1500-2000Lx.
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| CN107278414B (en) * | 2017-07-07 | 2023-05-16 | 陕西省西安植物园 | Method for promoting germination of rare endangered plant water chestnut seeds |
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| PEG引发种子的研究进展;崔秀敏等;《塔里木农垦大学学报》;20001231;第12卷(第4期);47-52 * |
| 广金钱草种子发芽条件的研究;董青松等;《中国种业》;20061231(第5期);32-33 * |
| 广金钱草种子发芽试验的研究;董青松等;《种子》;20061130;第25卷(第11期);86-89 * |
| 海南省广金钱草种子的繁育技术和质量标准;杨春雨等;《中国药业》;20141120;第23卷(第22期);37-38 * |
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