CN115843487B - Pre-emergence treatment method of crisp melon seeds - Google Patents
Pre-emergence treatment method of crisp melon seeds Download PDFInfo
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a pre-emergence treatment method of crisp melon seeds, which comprises the following steps: (1) soaking the melon seeds in warm water; (2) Taking out the melon seeds treated in the step (1), washing the melon seeds with distilled water, and then soaking the melon seeds in an aqueous solution of hydrolysis-resistant stable ion titanium; (3) Taking out the crisp melon seeds treated in the step (2), spreading and wrapping the crisp melon seeds in the wetted light-permeable sprouting paper, and then placing the crisp melon seeds in a culture dish; (4) Placing the culture dish in the step (3) in a red and blue light alternate irradiation environment for germination acceleration treatment; (5) And (5) observing germination conditions of the crisp melon seeds, and sowing the seeds after the seeds germinate with white exposure. The invention utilizes the novel mode of seed treatment and addition of hydrolysis-resistant stable ion titanium, effectively improves the germination rate, germination vigor, radicle length and seedling strengthening index of the crisp melon seeds, obtains proper hypocotyl length, avoids lodging in the later stage, and has the effects of seed disinfection, insect prevention and disease prevention and consistent growth vigor.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to a pre-emergence treatment method of crisp melon seeds.
Background
The melon (Culumis melo L.var. Connomon Group) is a variety of melon seeds of the genus melon of the family Cucurbitaceae, also known as claw honey, and is popular with local consumers in Jianghuai river basin of the origin Anhui province. Along with the improvement of the living standard of people, the demand for the crisp melons is continuously increased, so that melon farmers continuously expand the planting area of the crisp melons. As early as 2014, the production area of the crisp melon in the Pan-ji region of Huainan city is enlarged to 25000 mu, and the number of farmers is about 4000.
Along with the increase of market demands, the demand for crisp melon seedlings is increased continuously, so that how to cultivate high-quality commercial seedlings becomes a problem to be solved urgently, wherein the seedling raising link of seeds is particularly important, but the conventional mode of wrapping seeds with cloth after soaking for a period of time has the disadvantages of low germination rate and germination vigor, inconsistent germination time, uneven growth vigor, overgrowth and lodging, and a plurality of diseased seedlings, weak seedlings and high seedlings bring about no small loss and trouble to seedling raising.
Disclosure of Invention
The invention mainly aims to provide a pre-emergence treatment method for melon seeds, which can improve the germination rate and the germination vigor, ensure that the germination time is consistent and can obtain melon seeds with proper embryo axial length.
In order to achieve the above purpose, the invention provides a pre-emergence treatment method of crisp melon seeds, which comprises the following steps:
(1) Soaking the crisp melon seeds in warm water;
(2) Taking out the melon seeds treated in the step (1), washing the melon seeds with distilled water, and then soaking the melon seeds in an aqueous solution of hydrolysis-resistant stable ion titanium;
(3) Taking out the crisp melon seeds treated in the step (2), spreading and wrapping the crisp melon seeds in the wetted light-permeable sprouting paper, and then placing the crisp melon seeds in a culture dish;
(4) Placing the culture dish in the step (3) in a red and blue light alternate irradiation environment for germination acceleration treatment;
(5) And (5) observing germination conditions of the crisp melon seeds, and sowing the seeds after the seeds germinate with white exposure.
Further, in the step (1), the warm water is distilled water, the temperature is 50-55 ℃, and the soaking treatment time is 10-15 minutes.
Further, in the step (2), the concentration of titanium ions of the hydrolysis-resistant stable ion titanium aqueous solution is 5-15 mg/L, and the dosage is 5-6 times of the volume of the melon seeds.
Further, in the step (2), the soaking treatment is to soak for 6 to 12 hours at the temperature of 25 to 30 ℃.
In the step (3), filter paper is used as the sprouting paper.
In the step (3), the laying and wrapping mode is to lay the crisp melon seeds on the surface of a layer of wetted sprouting paper, and then cover a layer of wetted sprouting paper on the crisp melon seeds.
Further, in the step (4), the temperature condition of germination accelerating treatment is 25-30 ℃.
Further, in the step (4), the wavelength of red light alternately irradiated by red light and blue light is 400-520 nm, the single irradiation time is 10-14 hours, the wavelength of blue light is 610-720 nm, and the single irradiation time is 10-14 hours.
Further, in the step (4), the germination accelerating process supplements water to the germination paper to maintain the wet state.
The hydrolysis resistant stable ion titanium has the function of resisting bacteria, can ensure that the germination rate of seeds is high, the seeds are not easy to mildew and rot in soil, simultaneously, the radicle of the seeds is elongated, the growth speed of seedlings is accelerated, and the root development and the branch and leaf growth of plants can be promoted in the later stage of the plants.
The red light is favorable for sugar synthesis, the carbohydrate synthesis, accelerating and improving the stem node development of plants, the redundant energy is converted into heat energy, so that the water is evaporated, the blue light is favorable for protein synthesis, has great influence on the growth of plants and the formation of buds, can inhibit the elongation of the plants to enable the plants to form a short and strong shape, can also govern cell differentiation, and is favorable for anthocyanin and vitamin synthesis.
The beneficial effects of the invention are as follows:
the invention uses hydrolysis resistant stable ionic titanium aqueous solution, in which H 2 O as Ti 4+ And is oxidized to O 2 And H + . Thus, titanium ions can inhibit the proliferation of pathogenic bacteria, promote stress resistance in plants, and may be related to redox properties. The titanium compound has growth promoting effect on crops, can improve plant biomass, various enzyme activities, chlorophyll content, iron content and other key elements, and can be used for compensating nitrogen deficiency.
In addition, the invention discovers that proper hypocotyl length can be obtained by combining the germination accelerating treatment and the hydrolysis resistant stable ion titanium aqueous solution treatment in the red and blue light alternate irradiation environment for treating the crisp melon seeds, and the lodging of the crisp melon in the later stage can be avoided.
The pre-emergence treatment method for the crisp melon seeds effectively improves the germination rate, the germination vigor, the radicle length and the strong seedling index of the crisp melon seeds by utilizing the novel mode of seed treatment and addition of hydrolysis-resistant stable ion titanium, obtains proper hypocotyl length, avoids lodging in the later stage, and has the effects of disinfecting seeds, preventing insects and diseases and keeping the vigor consistent.
Drawings
FIG. 1 is a graph showing the germination rate of three melon varieties subjected to hydrolysis resistance treatment in test one compared with three melon varieties not subjected to hydrolysis resistance stable ion titanium treatment, wherein cont represents untreated melon seeds and ASIT represents treated melon seeds.
FIG. 2 is a graph comparing germination vigor of three melon varieties subjected to hydrolysis resistance treatment with three melon varieties not subjected to hydrolysis resistance stable ion titanium treatment in experiment one, wherein cont represents untreated melon seeds and ASIT represents treated melon seeds.
FIG. 3 is a graph showing germination comparison of three melon varieties subjected to hydrolysis-resistant treatment in experiment one with melon seeds not subjected to hydrolysis-resistant stable ion titanium treatment, wherein cont represents untreated melon seeds and ASIT represents treated melon seeds.
Fig. 4 is a graph comparing the growth of three melon varieties subjected to hydrolysis-resistant treatment with that of melon which has not been subjected to hydrolysis-resistant stable ion titanium treatment in experiment one, wherein cont represents untreated melon seeds and ASIT represents treated melon seeds.
Detailed Description
The invention is further described below with reference to examples:
the various materials and equipment used in the examples and experiments below, unless otherwise specified, are commercially available products well known in the art. Wherein the hydrolysis-resistant stable ion titanium is Taigule hydrolysis-resistant stable ion titanium purchased from Middling titanium agriculture chemical (Tianjin) technology Co., ltd; the filter paper is a qualitative filter paper purchased from national pharmaceutical chemicals company, inc. 102; examples 1 to 6 and experiments two and three used melon seeds were fruit type sheep horn crisp-crisp seven purchased from Huai south city, the red army species company, inc.
Example 1
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in distilled water at 55deg.C for 10 min, and stirring to make the seeds fully contact with water;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution at the temperature of 28 ℃ for 6 hours, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 5 times of the volume of the melon seeds, and the concentration of titanium ions is 5mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) The culture dish in the step (3) is placed in a red-blue light alternate irradiation environment for germination, the wavelength of red light is 500nm, the single irradiation time is 12 hours, the wavelength of blue light is 700nm, the single irradiation time is 12 hours, the red light irradiation is started, the red-blue light alternate irradiation is carried out, the temperature is kept at 28 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Example 2
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in distilled water at 55deg.C for 10 min, and stirring to make the seeds fully contact with water;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution for 9 hours at the temperature of 28 ℃, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 5 times of the volume of the melon seeds, and the concentration of titanium ions is 7mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) Placing the culture dish in the step (3) in a red-blue light alternate irradiation environment for germination, firstly carrying out red light irradiation, then carrying out blue light irradiation, wherein the wavelength of red light is 480nm, the single irradiation time is 12 hours, the wavelength of blue light is 650nm, the single irradiation time is 12 hours, the red light irradiation is started, the red light and the blue light alternate irradiation is carried out, the temperature is kept at 28 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Example 3
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in distilled water at 55deg.C for 10 min, and stirring to make the seeds fully contact with water;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution at the temperature of 25 ℃ for 12 hours, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 6 times of the volume of the melon seeds, and the concentration of titanium ions is 10mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) Placing the culture dish in the step (3) in a red-blue light alternate irradiation environment for germination, firstly carrying out red light irradiation, then carrying out blue light irradiation, wherein the wavelength of red light is 520nm, the single irradiation time is 12 hours, the wavelength of blue light is 720nm, the single irradiation time is 12 hours, the red light irradiation is started, the red light and the blue light alternate irradiation is carried out, the temperature is kept at 25 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Example 4
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in warm water at 50deg.C for 15 min, and stirring to make the seeds fully contact with water until the water temperature is reduced to normal temperature;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution at the temperature of 28 ℃ for 6 hours, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 6 times of the volume of the melon seeds, and the concentration of titanium ions is 5mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) Placing the culture dish in the step (3) in a red-blue light alternate irradiation environment for germination, firstly carrying out red light irradiation, then carrying out blue light irradiation, wherein the wavelength of red light is 400nm, the single irradiation time is 12 hours, the wavelength of blue light is 610nm, the single irradiation time is 12 hours, the red light irradiation is started, the red-blue light alternate irradiation is carried out, the temperature is kept at 28 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Example 5
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in warm water at 50deg.C for 15 min, and stirring to make the seeds fully contact with water until the water temperature is reduced to normal temperature;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution for 9 hours at the temperature of 30 ℃, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 5 times of the volume of the melon seeds, and the concentration of titanium ions is 12mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) Placing the culture dish in the step (3) in a red-blue light alternate irradiation environment for germination, firstly carrying out red light irradiation, then carrying out blue light irradiation, wherein the wavelength of red light is 450nm, the single irradiation time is 10 hours, the wavelength of blue light is 650nm, the single irradiation time is 14 hours, the red light irradiation is started, the red light and the blue light alternate irradiation is carried out, the temperature is kept at 30 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Example 6
The pre-emergence treatment method of the crisp melon seeds comprises the following steps:
(1) Removing impurities from mature and uniform crisp melon seeds, soaking in warm water at 50deg.C for 15 min, and stirring to make the seeds fully contact with water until the water temperature is reduced to normal temperature;
(2) Taking out the melon seeds treated in the step (1), slightly rubbing seed coats, washing the seed coats with distilled water, then putting the seed coats into an hydrolysis-resistant stable ion titanium aqueous solution, and soaking the seed coats in the hydrolysis-resistant stable ion titanium aqueous solution at the temperature of 30 ℃ for 12 hours, wherein the dosage of the hydrolysis-resistant stable ion titanium aqueous solution is 5 times of the volume of the melon seeds, and the concentration of titanium ions is 15mg/L;
(3) Taking out the melon seeds treated in the step (2), spreading the melon seeds on the surface of the wetted filter paper, covering a layer of wetted filter paper on the melon seeds, and putting the melon seeds into a glass culture dish;
(4) Placing the culture dish in the step (3) in a red-blue light alternate irradiation environment for germination, firstly carrying out red light irradiation, then carrying out blue light irradiation, wherein the wavelength of red light is 500nm, the single irradiation time is 14 hours, the wavelength of blue light is 650nm, the single irradiation time is 10 hours, the red light irradiation is started, the red light and the blue light alternate irradiation is carried out, the temperature is kept at 30 ℃, and distilled water is sprayed to the surface of filter paper by a watering can every day in the germination process to keep the melon seeds in a moist state;
(5) And (5) timely observing the germination condition of the seeds, and sowing the seeds after the seeds germinate in a white state.
Test one
Influence of hydrolysis-resistant stable ion titanium aqueous solution on germination and seedling growth of different varieties of melon seeds
Three types of crisp melon seeds, namely fruit type crisp-crisp seven types (hereinafter referred to as crisp seven types) purchased from the limited company of the red army seed industry in the south of China, green-collar type crisp (hereinafter referred to as green-collar type crisp) purchased from the limited company of the green-collar type seed industry in the south of Beijing, and Pu Tian Run brand crisp and sweet sheep (hereinafter referred to as Pu Tian Run Yan Ke) purchased from the limited company of the source seed industry in the Ying county, were treated respectively as experimental groups, and step (2) was omitted from the method of example 1 to treat three types of crisp melon seeds, namely crisp seven types, green-collar type crisp and Pu Tian Run type crisp, respectively, as control groups.
The test was carried out by bagging 200 grains/part for each, and testing 18 parts of melon seeds for three materials, 9 parts of the test group and the other 9 parts of the control group, and repeating the above test 3 times for each material.
And 9 crisp melon seeds of an experimental group and 9 crisp melon seeds of a control group are duly sowed in the plug tray while growth indexes such as germination rate, germination vigor and the like are measured, and the seedling vigor is observed according to the conventional management of seedling culture.
The test results are shown in fig. 1-4, wherein cont represents untreated melon seeds and ASIT represents treated melon seeds. The result shows that the treatment method has remarkable promotion effect on the aspects of the germination rate, the germination vigor, the seedling growth vigor and the like of the crisp melon seeds.
Test II
Influence of different seed soaking times and different hydrolysis resistance stable ion titanium concentrations on seed germination and seedling growth of crisp melon
The crisp melon seeds of example 2 were treated by the pretreatment method before germination, and the soaking times of 0, 6, 9, and 12 hours for the hydrolysis-resistant stable ion titanium aqueous solution were respectively set for two changing factors of 0, 5, 7, and 10mg/L titanium ion concentration.
Timely observing the germination condition of seeds, recording the germination vigor of the seeds after 1d, recording the germination rate, radicle length and embryo axis length of the seeds after 3d, sowing the seeds in a plug tray at proper time, observing the growth vigor of plants after 30d according to the conventional management of seedling culture, and recording the growth index. The test was randomly bagged for use at 200 seeds per serving and repeated 3 times. The results of the implementation are shown in tables 1 and 2.
TABLE 1 influence of treatments with different seed soaking time and different hydrolysis resistance stabilizing ion titanium concentrations on seed germination of melon
TABLE 2 influence of treatments with different seed soaking time and different hydrolysis-resistant stable ion titanium concentrations on melon seedling growth
The result shows that the treatment method has obvious promotion effect on germination indexes such as germination rate, germination potential, radicle length, hypocotyl length and the like of the crisp melon seeds, and has good influence on seedling growth vigor such as root length, fresh weight, leaf area and strong seedling index, especially has uniform growth vigor, and the axial length of the three-day embryo can reach more proper 13-17 mm, can avoid overgrowth lodging in later stage, and is pleasant to see in seedling production. Wherein the combination of soaking seed for 9h and stabilizing ion titanium concentration for hydrolysis resistance of 7mg/L is optimal.
Test three
Influence of different illumination conditions on germination and seedling growth of melon seeds
The melon seeds of example 2 were treated for S5 XS 2 by the pretreatment method, and red light irradiation was performed for 24 hours, blue light irradiation was performed for 24 hours, and the germination accelerating light condition changing factor was set for 24 hours.
Based on example 2, the titanium ion concentration was changed to 0mg/L, the germination accelerating conditions were changed to 24 hours under dark conditions, and other conditions were unchanged, to serve as a control group.
Timely observing the germination condition of seeds, recording the germination vigor of the seeds after 1d, recording the germination rate, radicle length and embryo axis length of the seeds after 3d, sowing the seeds in a plug tray at proper time, observing the growth vigor of plants after 30d according to the conventional management of seedling culture, and recording the growth index. The test was randomly bagged for use at 200 seeds per serving and repeated 3 times. The results of the implementation are shown in tables 3 and 4.
TABLE 3 influence of different illumination conditions on seed germination of melon
Illumination conditions | Germination percentage/100% | Germination potential/100% | Radicle length/mm | Embryo axial length/mm |
Red light irradiation for 24 hours | 98.00 | 94.50 | 28.60 | 16.80 |
Blue light irradiation for 24 hours | 99.00 | 95.33 | 31.50 | 14.28 |
Light-shielding for 24 hours | 96.00 | 90.67 | 24.00 | 11.60 |
Control group | 80.30 | 73.00 | 21.50 | 10.28 |
TABLE 4 influence of different illumination conditions on melon seedling growth
Illumination conditions | Root length/mm | Fresh weight/g | Leaf area/mm 2 | Index of strong seedlings |
Red light irradiation for 24 hours | 12.89 | 7.69 | 2200 | 9.2 |
Blue light irradiation for 24 hours | 16.57 | 7.81 | 2055 | 9.5 |
Light-shielding for 24 hours | 11.91 | 6.14 | 1915 | 9.0 |
Control group | 10.89 | 6.02 | 1818 | 7.4 |
The result shows that the red and blue light irradiation can improve the germination condition and seedling growth vigor of the melon seeds to different degrees, wherein the red light can promote the growth and development of the overground parts of plants, the blue light can promote the growth of the underground parts of the plants, and the stem node overgrowth is inhibited. The red and blue light irradiation and hydrolysis resistant stable ionic titanium aqueous solution treatment can promote seed germination and also can play a role in preventing high-foot lodging in the seedling stage of crisp melon.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the invention.
Claims (4)
1. The pre-emergence treatment method of the crisp melon seeds is characterized by comprising the following steps of:
(1) Soaking the crisp melon seeds in warm water;
in the step (1), the warm water is distilled water, the temperature is 50-55 ℃, and the soaking treatment time is 10-15 minutes;
(2) Taking out the melon seeds treated in the step (1), washing the melon seeds with distilled water, and then soaking the melon seeds in an aqueous solution of hydrolysis-resistant stable ion titanium;
in the step (2), the titanium ion concentration of the hydrolysis-resistant stable ion titanium aqueous solution is 5-15 mg/L, and the dosage is 5-6 times of the volume of the melon seeds; the soaking treatment is to soak for 6 to 12 hours at the temperature of 25 to 30 ℃;
(3) Taking out the crisp melon seeds treated in the step (2), spreading and wrapping the crisp melon seeds in the wetted light-permeable sprouting paper, and then placing the crisp melon seeds in a culture dish;
(4) Placing the culture dish in the step (3) in a red and blue light alternate irradiation environment for germination acceleration treatment;
in the step (4), the temperature condition of germination accelerating treatment is 25-30 ℃; the wavelength of red light alternately irradiated by red light and blue light is 400-520 nm, the single irradiation time is 10-14 hours, the wavelength of blue light is 610-720 nm, and the single irradiation time is 10-14 hours;
(5) And (5) observing germination conditions of the crisp melon seeds, and sowing the seeds after the seeds germinate with white exposure.
2. The method of pre-emergence treatment of melon seeds according to claim 1 wherein in step (3), the sprouting paper is filter paper.
3. The method of pre-emergence treatment of melon seeds according to claim 1, wherein in step (3), the method of laying and wrapping in step (3) is to lay the melon seeds on a layer of wetted sprouting paper surface, and then cover the melon seeds with a layer of wetted sprouting paper.
4. The method of pre-emergence treatment of melon seeds according to claim 1 wherein in step (4), the germination accelerating process supplements the germination paper with water to maintain a moist state.
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