CN104521957A - Applications of guaiacol in rice stripe disease - Google Patents
Applications of guaiacol in rice stripe disease Download PDFInfo
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- CN104521957A CN104521957A CN201410745727.2A CN201410745727A CN104521957A CN 104521957 A CN104521957 A CN 104521957A CN 201410745727 A CN201410745727 A CN 201410745727A CN 104521957 A CN104521957 A CN 104521957A
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Abstract
The present invention discloses applications of guaiacol in a rice stripe disease, wherein the applying concentration of the guaiacol liquid is 50-500 [mu]g/mL, and an emulsifying agent is added to the guaiacol liquid as a surfactant. According to the present invention, the guaiacol is used for rice stripe disease prevention and treatment, the rice stripe disease prevention and treatment effect of the guaiacol can achieve 53%, and the guaiacol is combined with the chemical pesticide for virus transmission mediator Laodelphax striatellus prevention and treatment so as to achieve the effect of concurrent disease and pest treatment; and experiment results prove that the guaiacol can be used for effectively preventing and treating the rice stripe disease.
Description
Technical field
The invention belongs to technical field of pesticide, relate to the novelty teabag of guaiacol, be specifically related to the application of guaiacol in stripe disease.
Background technology
Stripe disease is vector-borne virus disease by small brown rice planthopper, is commonly called as the cancer on paddy rice.The normal withered booting of diseased plant or fringe minor malformation unreal.There is yellow green striped in sword-like leave bottom in jointing sequela, all not withered heart of all types of rice, but heading deformity, seldom solid.Rice stripe virus is called for short RSV, claims oryza virus 3, belongs to rice stripe virus group (or claiming soft silk virus group) virus.Virion is thread, size 400 × 8nm, is scattered in cytoplasm, vacuole and core, or becomes the unsetting glomeration such as graininess, sand shape, i.e. inclusion body, seemingly has many filamentouss to tangle and agglomerating.Sick leaf sap dilution point of accumulation 1000 ~ 10000 times, passivation temperature be 55 DEG C 3 minutes, subzero 20 DEG C, the longevity in vitro (sick rice) 8 months.The loss caused time serious can reach 80%, is one of disease of serious harm rice harves.
This virus is only by amboceptor insect-borne infection, and other approach does not pass disease.Amboceptor insect is mainly small brown rice planthopper, once obtain poison can pass poison through ovum all the life, though can pass poison as white ridge plant hopper at natural world, effect is little.10 minutes the shortest drug abuse time, walk around to phase 4-23 days, general 10-15 days.Virus, also can through ovum transmission in polypide internal breeding.Virus flies to survive the winter in body at the malicious ash of band, becomes main primary source of infection.The nymph that large and small wheatland is survived the winter, breed after emergence at former wheatland, then migrating passes poison to early rice rice seedling bed or Honda and causes harm and breed, and after early rice results, then migrates and to cause harm to late rice, after late rice results, move back on winter wheat and survive the winter.Paddy rice is easily susceptible in seedling stage to tillering stage.The leaf age long incubation period is also longer, and with plant strain growth, resistance strengthens gradually.At present, in production, main Bian 48% chlopyrifos long-acting sustained-release agent, 20% poison are pungent, and by 0.1% seed dressing dressing seed of grain weight, preventive effect can reach more than 50%; Or adopt " Tianda-2116 "+sky to reach abundant rich (bacterium speed is killed) 30 grams, be watered 30 kilograms, even spraying diseases prevention; Or the anti-stripe disease of eugenol even spraying, wherein, the eugenol aqueous emulsion of 20% is respond well to water prevention rice stripe.
Guaiacol (guaiacol), structural formula is as follows:
Formal name used at school o-methoxyphenol, also known as methyl catechol, guaiacol, a kind of white or micro-yellow crystal or colourless to pale yellow transparent oily liquids, there is special aromatic odor, occurring in nature is present in guaiac resin, pine tar and hardwood empyreumatic oil, be a kind of important fine-chemical intermediate, be widely used in the synthesis of medicine, spices and dyestuff.
Wherein, guaiacol is mainly the good resolve phlegm effect of guaiacol glycerol ether in purposes pharmaceutically, and the structural formula of guaiacol glycerol ether is as follows:
Stomach lining can be stimulated after guaiacol glycerol ether is oral, reflectivity causes bronchial secretion to increase, and sputum is diluted, and the many productive coughs for chronic bronchitis are coughed, pulmonary abscess, bronchiectasis and Secondary cases asthma, itself and antitussive and antiasthmatic medicine share, for the situation of mucus not easily expectoration.
At present, in open source literature, do not retrieve the corresponding data of guaiacol in stripe disease control and application.
Summary of the invention
The object of this invention is to provide the novelty teabag of guaiacol, namely the application of guaiacol in stripe disease is prevented and treated be provided.
The technical solution adopted in the present invention is as follows:
Guaiacol is in the application of stripe disease.
When guaiacol is as water prevention rice stripe, its spraying medicine concentration is 50 ~ 500 μ g/mL, and spraying medicine concentration is 200 ~ 400 μ g/mL more preferably.
On the basis of technique scheme, for improving solvability, the raising antiviral effect of guaiacol, emulsifier can be added as surfactant in guaiacol liquid.
Above-mentioned guaiacol liquid, when preparing, is first got the guaiacol of 40%, is dissolved as the mother liquor of 5000 ~ 20000 μ g/mL with ethanol, then to add emulsifier aqueous solution as surface-active dilution agent be 50 ~ 400 μ g/mL.
The present invention is when dispenser, and the guaiacol liquid getting 50 ~ 500 μ g/mL carries out spray pesticide to seedling cultivation of rice rice shoot, and spray value is 200 ~ 1000L/h m
2, atomisation pressure is 0.3 ~ 0.8kPa, and spraying times is 1 ~ 3 time.
The guaiacol liquid mechanism of action:
(1) suppress the activity of RNA reverse transcriptase RTASE, stop virion synthesis;
(2) stop the absorption of virion on cell, reduce virus and contaminate probability;
(3) strengthen the oxidation resistance in plant corpus, reduce the injury of virus dip-dye to plant;
(4) resistance against diseases of plant is improved.
Compared with prior art, the present invention has following beneficial effect:
Guaiacol is used for the control of stripe disease by the present invention, and guaiacol can reach 53% to the control efficiency of stripe disease, passes the chemical pesticide of virus mediator small brown rice planthopper, thus reach the double effect controlled of disease worm with the use of control.Prove by experiment, for stripe disease, guaiacol can be used to carry out effective prevention and therapy.
Embodiment
For the applicable cases of guaiacol of the present invention in stripe disease is described, checking guaiacol is to the control efficiency of stripe disease, get the guaiacol liquid of four variable concentrations respectively as embodiment 1,2,3 and 4, and with 20% eugenol (eugnol) aqueous emulsion as a comparison case, at indoor measurement guaiacol liquid and 20% eugenol aqueous emulsion to the control efficiency of stripe disease, specific as follows:
1, medicament
Experimental pharmacy: 40% guaiacol stoste, is provided by Anhui Yangzi Chemical Industry Co., Ltd.;
Contrast medicament 20% eugenol (eugnol) aqueous emulsion, Safety of Food Quality research institute of academy of agricultural sciences of Jiangsu Province provides.
2, for examination target
2.1 subjects:
Stripe disease disease (Rice stripe virus(RSV)).
2.2 for prelibation source:
Hold virus research room, Institute of Plant Protection of academy of agricultural sciences of Jiangsu Province with the RSV biography virus mediator small brown rice planthopper of malicious rate more than 50% to provide.
2.2 test plants:
Rice paddy seed (kind: Wu-Yu-Geng 3) floods 48h in clear water, and after showing money or valuables one carries unintentionally, be seeded in (soil organic matter content is 1.42%, pH6.7) in the plastic basin of diameter 6cm 28 DEG C of vernalization, every basin sows 20, keeps ground moistening with bottom way of supplying water.The cultivation of rice seedling is carried out in illumination cultivation room (25 DEG C, relative moisture is 70%, 8D/16L illumination).In rice seedling 1.5 ~ 2 leaf phase, every basin stays the consistent 10 strain rice shoots of growth for spraying medicine and connecing worm.
3, processing method is tested
The preparation of 3.1 liquids
Get the mother liquor that 40% guaiacol mother liquor ethanol is dissolved into 10000 μ g/mL, also can become the mother liquor of 10000 μ g/mL by other dissolution with solvents such as the guaiacol mother liquor methyl alcohol of other concentration; And in liquid mother liquor, add emulsifier make surfactant;
20% eugenol aqueous emulsion is directly diluted to the mother liquor of 20000 μ g/mL with clear water, and in liquid mother liquor, adds emulsifier make surfactant.
The setting of concentration: with the mother liquor of the aqueous solution dilution reagent agent of emulsifier; Guaiacol mother liquor and eugenol are the liquid of 400,200,100,50 μ g/mL by liquor strength final after proportional diluted; Each concentration establishes 4 repetitions, often repeats spraying 10 strain rice seedlings.
3.2 test method
Carry out with reference to " agricultural chemicals indoor biometrics test rule bactericide the 4th part: control wheat powdery mildew test pot-culture method ", adopt cauline leaf spray-on process to measure medicament to the control efficiency of stripe disease disease.
3.2.1 reagent spray method
Rice seeding tray is placed in PWT-510 type spray tower, and (precision spraying area is 0.2m for the guaiacol of variable concentrations of spraying from low to high and eugenol liquid
2, spraying liquid 10mL, spray value is 500L/h m
2, atomisation pressure is 0.5kPa).After spray medicine rice seedlings moves to 25 DEG C, relative temperature is in the illumination greenhouse of 70%.
3.2.2 inoculation method
After spray medicine, the rice seedling basin of 24h is placed in 1000mL glass beaker, rim of a cup nylon gauze tying; Effectively meet worm amount access RSV by 4 heads/plant and pass virus mediator small brown rice planthopper 2-4 Age nymph, catch up with worm every day 2 times, to guarantee that rice seedling evenly obtains poison.After connecing worm, small brown rice planthopper all removes by 48h, and rice seedling is still placed in the illumination growth room of above-mentioned condition, keeps ground moistening with bottom way of supplying water.
3.2.3 investigation method
32d after inoculation, according to (plant protection, 2008,34(6) such as Zhou Tong: method 77-80) investigates the sick severity of each process rice stripe dead leaf:
0 grade: non-evident sympton;
1 grade: have the mottled symptom of slight yellow green, sick leaf is not curling, and plant strain growth is normal;
2 grades: on sick leaf, chlorisis expansion is connected to irregular yellow-white or yellow green streak, and sick leaf is not curling or slightly curling, grows normal;
3 grades: the serious chlorisis of sick leaf, sick leaf roll song is in twisted shape, and yellow wilting symptoms appears in the sick leaf of minority;
4 grades: most of sick leaf roll song is in twisted shape, and yellowing leaf is withered, and it is withered that plant is false withered heart shaped or whole strain.
4, data statistics and analysis
4.1 computational methods
Disease index=(Σ (diseased plant number × relative level numerical value at different levels)/(investigating total strain number × the highest sick level value)) × 100
Control efficiency (%)=((contrast disease index-process disease index)/contrast disease index) × 100
According to the preventive effect probability value regression analysis of each drug concentration logarithm value and correspondence, ask virulence regression equation and calculate EC
50.All calculating is all carried out with DPS data processing software.
5, result of the test
5.1 control efficiency
Adopt cauline leaf mist measuring guaiacol, eugenol to the preventive effect of stripe disease disease, each medicament variable concentrations to the control efficiency of stripe disease disease in table 1.
table 1: guaiacol and eugenol are to the control efficiency (cauline leaf spray-on process) of stripe disease disease
From table 1 result of the test, guaiacol is to the control efficiency of stripe disease disease higher than eugenol, and particularly embodiment 1(guaiacol liquor strength is 400 μ g/mL) control efficiency can reach more than 53%.
5.2 50 7.509085e-218ffective concentration (EC
50)
Each medicament is calculated to the virulence regression equation of stripe disease disease and 50 7.509085e-218ffective concentration (EC by probit value method
50).
Guaiacol is to the toxicity regression formula of stripe disease:
Y=2.3642+1.05545x （r=0.9903）
EC
50=314.2813 μ g/mL(95% confidence limits: 124.3359 ~ 1198.7324 μ g/mL)
Eugenol is to the toxicity regression formula of stripe disease:
Y=2.8595+0.7990x (r=0.9852)
EC
50=477.4463 μ g/mL(95% confidence limits: 141.1283 ~ 3397.1965 μ g/mL)
Result of the test shows that the control efficiency of guaiacol to stripe disease is higher than eugenol.Guaiacol is to the EC of stripe disease
50value is 314.2813 μ g/mL, the EC of eugenol
50value is 477.4463 μ g/mL, and result shows that the activity of guaiacol is 1.52 times of eugenol.
Claims (6)
1. guaiacol is in the application of stripe disease.
2. guaiacol as claimed in claim 1 is in the application of stripe disease, it is characterized in that: the spraying medicine concentration of described guaiacol liquid is 50 ~ 500 μ g/mL.
3. guaiacol as claimed in claim 1 is in the application of stripe disease, it is characterized in that: the spraying medicine concentration of described guaiacol liquid is 200 ~ 400 μ g/mL.
4. guaiacol as claimed in claim 1 is in the application of stripe disease, it is characterized in that: in described guaiacol liquid, add emulsifier as surfactant.
5. guaiacol as claimed in claim 1 is in the application of stripe disease, it is characterized in that: described guaiacol liquid is when preparing, first guaiacol ethanol is dissolved as the mother liquor of 5000 ~ 20000 μ g/mL, then add emulsifier aqueous solution dilution be 50 ~ 500 μ g/mL.
6. guaiacol as claimed in claim 1 is in the application of stripe disease, and it is characterized in that, during dispenser, the guaiacol liquid getting 50 ~ 500 μ g/mL carries out spray pesticide to seedling cultivation of rice rice shoot, and spray value is 200 ~ 1000L/h m
2, atomisation pressure is 0.3 ~ 0.8kPa, and spraying times is 1 ~ 3 time.
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| CN201410745727.2A CN104521957A (en) | 2014-12-09 | 2014-12-09 | Applications of guaiacol in rice stripe disease |
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