CN105746516A - Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen - Google Patents
Application of denatonium benzoate in preparing bactericide for preventing and controlling plant diseases caused by phytopathogen Download PDFInfo
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- CN105746516A CN105746516A CN201610191166.5A CN201610191166A CN105746516A CN 105746516 A CN105746516 A CN 105746516A CN 201610191166 A CN201610191166 A CN 201610191166A CN 105746516 A CN105746516 A CN 105746516A
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- denatonium benzoate
- preventing
- antibacterial
- bacterium
- pathogenic
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- VWTINHYPRWEBQY-UHFFFAOYSA-N denatonium Chemical compound [O-]C(=O)C1=CC=CC=C1.C=1C=CC=CC=1C[N+](CC)(CC)CC(=O)NC1=C(C)C=CC=C1C VWTINHYPRWEBQY-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 229960001610 denatonium benzoate Drugs 0.000 title claims abstract description 64
- 201000010099 disease Diseases 0.000 title claims abstract description 53
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 53
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 33
- 239000003899 bactericide agent Substances 0.000 title abstract description 9
- 230000001717 pathogenic effect Effects 0.000 claims abstract description 31
- 244000052769 pathogen Species 0.000 claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims description 50
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- 235000003095 Vaccinium corymbosum Nutrition 0.000 claims description 22
- 235000017537 Vaccinium myrtillus Nutrition 0.000 claims description 22
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- 244000052616 bacterial pathogen Species 0.000 claims description 21
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- 239000001606 7-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]oxy-5-hydroxy-2-(4-hydroxyphenyl)chroman-4-one Substances 0.000 description 1
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- 241000272814 Anser sp. Species 0.000 description 1
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- 241000033870 Citrullus lanatus subsp. vulgaris Species 0.000 description 1
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- 235000005590 Larix decidua Nutrition 0.000 description 1
- NNJVILVZKWQKPM-UHFFFAOYSA-N Lidocaine Chemical compound CCN(CC)CC(=O)NC1=C(C)C=CC=C1C NNJVILVZKWQKPM-UHFFFAOYSA-N 0.000 description 1
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- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
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- RRKTZKIUPZVBMF-IBTVXLQLSA-N brucine Chemical compound O([C@@H]1[C@H]([C@H]2C3)[C@@H]4N(C(C1)=O)C=1C=C(C(=CC=11)OC)OC)CC=C2CN2[C@@H]3[C@]41CC2 RRKTZKIUPZVBMF-IBTVXLQLSA-N 0.000 description 1
- RRKTZKIUPZVBMF-UHFFFAOYSA-N brucine Natural products C1=2C=C(OC)C(OC)=CC=2N(C(C2)=O)C3C(C4C5)C2OCC=C4CN2C5C31CC2 RRKTZKIUPZVBMF-UHFFFAOYSA-N 0.000 description 1
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- DFPMSGMNTNDNHN-ZPHOTFPESA-N naringin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC=2C=C3O[C@@H](CC(=O)C3=C(O)C=2)C=2C=CC(O)=CC=2)O[C@H](CO)[C@@H](O)[C@@H]1O DFPMSGMNTNDNHN-ZPHOTFPESA-N 0.000 description 1
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- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of denatonium benzoate in preparing a bactericide for preventing and controlling plant diseases caused by phytopathogen.It is proved through indoor toxicity measurement that denatonium benzoate has good inhibitory activity to phytopathogen.Serving as a bactericide, denatonium benzoate has the advantages of being efficient and low in toxicity and meeting the requirement for chemically preventing and controlling plant diseases.At present, bactericide resistance of pathogen is enhanced as a great number of bactericides are used, and the traditional bactericides pollute the environment seriously, are high in residual amount and directly threat food safety of human.Denatonium benzoate is a small molecule compound which is degradable, free of pollution and environmentally friendly, is low in bactericide resistance, safe to non-target organisms, people and livestock and capable of ensuring high quality of agricultural products, fruits and vegetables, meets the requirement for sustainable development and has wide research and market application prospects.
Description
Technical field
The present invention relates to tool Fungicidal active compound, particularly to denatonium benzoate in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.
Background technology
Enter new century; facing mankind more challenge and crisis; the problems such as wherein population increase, shortage of resources, environmental pollution, ecological disruption and frequent natural calamity become increasingly conspicuous, and seriously hinder the sustainable development of agricultural, and therefore agricultural production also undertakes unprecedented immense pressure.Estimating according to FAO (Food and Agriculture Organization of the United Nation), global grain and Cotton Production are lost in more than 10% because of disease throughout the year.Plant disease not only can cause the minimizing of crop yield, and to a certain extent also serious threat to the quality safety of agricultural product and international trade thereof.
From the angle controlling crop pest, current antibacterial has the irreplaceable effect of other materials, but its Drug resistance is all being increased the weight of by the drug injury problem of antibacterial and pathogen year by year, and its long-term a large amount of uses can cause environmental pollution, ecological balance to destroy.China's agricultural to develop towards healthy sustainable direction, it is necessary to develop efficient, low toxicity, low-residual and environmentally friendly antibacterial is applied in production practices.Along with environment, ecological concern are increased by the mankind increasingly, solve resistance, strengthen plant immunization ability, safe and efficient and environment compatibility well then becomes the direction of antibacterial production development.
Denatonium benzoate, has another name called bitter, chemical name: benzoic acid [2-[(2,6-3,5-dimethylphenyl) amino]-2-oxoethyl]-N, N-diethylbenzyl ammonium；English name: DenatoniumBenzoate, Benzenemethanaminium, N-[2-[(2,6-dimethylphenyl) amino]-2-oxoethyl]-N, N-diethyl-, benzoate；No. CAS: 3734-33-6；Molecular formula: C28H34N2O3；Molecular weight: 446.5882；Molecular structural formula is:
White crystalline powder, fusing point is 164-168 DEG C, odorless, has bitterness strongly, is highly soluble in water, ethanol, ethylene glycol etc., and its aqueous solution is in neutrality.Denatonium benzoate is to be currently known compound the most bitter in the world, if setting the bitter degree of quinine as benchmark 1, the bitter degree of this product is then 1000, concentration is the solution of 10ppm, it has been so bitter that cannot to stand for vast majority of people, its overwhelming majority application is all relevant with its bitterness, is the inexpensive efficient substitute of brucine, guassin, quinine, the bent glycoside of wood, naringin.
Denatonium benzoate is a kind of quaternary ammonium salt formed by quaternary ammonium salt cationic and inert anion such as benzoate anion ion or saccharin anion binding.The cationic structure of this material is similar with local anesthetic lidocaine, and its difference is only many benzyl functional groups on the nitrogen-atoms of amino.
By as bitters (or aversive agent) in the usual article of everyday use of denatonium benzoate and industrial goods, to avoid people to eat other poisonous tasteless material by mistake.Such as add in the middle of ethylene glycol or methanol, antifreezing agent, paint, detergent for toilet, liquid soap and the shampoo of industrial alcohol and common drinks mouthfeel similar, it is possible to prevent the generation of the poisoning that people, animal cause because eating by mistake and prevent animal from biting article (making consumption is 1～50ppm).In addition also can add in special nail polish, for avoiding the bad habit of child's fingernail biting, and expel the expellant of large-scale wild beast.Owing to working concentration is extremely low, it is safety non-toxic under normal conditions of use.
At present, there is no both at home and abroad about denatonium benzoate the agricultural inhibiting report of pathogen.
Summary of the invention
It is an object of the invention to provide denatonium benzoate in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.The present invention passes through toxicity test, it was demonstrated that plant pathogenic fungi is had a good inhibitory activity by denatonium benzoate.Denatonium benzoate is as antibacterial, and it is efficient, low toxicity, is suitable for the requirement of plant disease chemical prevention.
For achieving the above object, the present invention is achieved by the following technical solutions:
The invention provides denatonium benzoate in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.
Further: described phytopathogen is Deuteromycotina fungus, Ascomycotina fungus and Mastigomycotina fungus.
Further: in described antibacterial, effective working concentration of denatonium benzoate is 5mM-10mM.
Further: described Deuteromycotina fungus includes blue berry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blue berry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, Fructus Cucumidis sativi rod spore leaf spot fungi, verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium, cercospora black spot of peanut bacterium and Maize Curvularia.
Further: described Ascomycotina fungus includes blue berry branch ulcer bacteria, Botryosphaeria berengeriana f. sp, Valsa mali and pears rotten pathogenic bacteria.
Further: described Mastigomycotina fungus includes Lignum cinnamomi camphorae phytophthora root rot bacterium and tobacco black shank bacterium.
Further: when in described antibacterial, the concentration of denatonium benzoate is 10mM, denatonium benzoate to the suppression ratio of the 7 kind of plant pathogen such as botrytis cinerea, blue berry branch ulcer bacteria and Botryosphaeria berengeriana f. sp up to 99%.
Further: described plant is Lignum cinnamomi camphorae, Fructus Mali pumilae, Fructus Persicae, pears, Fructus Lycopersici esculenti, blue berry, Fructus Vitis viniferae, Nicotiana tabacum L., Fructus Cucumidis sativi, Cotton Gossypii, Oryza sativa L., Semen Maydis, Semen Tritici aestivi, Semen arachidis hypogaeae and Semen sojae atricolor.
Compared with prior art, advantages of the present invention and have the technical effect that the kind mechanism of action of current antibacterial is more single, the reproduction speed of pathogen is very fast, therefore resistance also produces comparatively fast, and pollutes greatly, and high residue threatens the food safety of the mankind.
The present invention passes through Toxicity Determination, it was demonstrated that plant pathogenic fungi is had a good inhibitory activity by denatonium benzoate.Denatonium benzoate, as antibacterial, has efficiently and the advantage of low toxicity, is suitable for the requirement of plant disease chemical prevention.The use of current bulk sterilization agent, causes that the Drug resistance of pathogen strengthens, and traditional antibacterial is big for environment pollution, residual height, directly threatens the food safety of the mankind.Along with cry pollution-free, pollution-free green food is improved by people day by day, green bactericide has become the important prevention and controls in agricultural production, and many green bactericide are come out one after another, and is widely used in producing, and achieves remarkable result.And denatonium benzoate is a kind of degradable, micromolecular compound pollution-free, environment amenable, and its Drug resistance is poor, to non-target organism and person poultry safety, ensure that the high-quality of agricultural product and fruit and vegerable, meet the requirement of sustainable development, its research and market application foreground are wide.
Accompanying drawing explanation
Fig. 1 is the bacteriostatic experiment result that in the present invention, blue berry is intended stem point branch-rot bacterium by denatonium benzoate；
Fig. 2 is the denatonium benzoate bacteriostatic experiment result to tomato early blight bacterium in the present invention；
Fig. 3 is the denatonium benzoate bacteriostatic experiment result to tobacco brown spot pathogen in the present invention；
Fig. 4 is the denatonium benzoate bacteriostatic experiment result to botrytis cinerea in the present invention；
Fig. 5 is the denatonium benzoate bacteriostatic experiment result to fusarium graminearum in the present invention；
Fig. 6 is the denatonium benzoate bacteriostatic experiment result to potato wilt bacterium in the present invention；
Fig. 7 is the denatonium benzoate bacteriostatic experiment result to Tobacco target spot bacterium in the present invention；
Fig. 8 is the denatonium benzoate bacteriostatic experiment result to Maize Curvularia in the present invention；
Fig. 9 is the denatonium benzoate bacteriostatic experiment result to Fructus Cucumidis sativi rod spore leaf spot fungi in the present invention；
Figure 10 is the denatonium benzoate bacteriostatic experiment result to blue berry branch-rot bacterium in the present invention；
Figure 11 is the denatonium benzoate bacteriostatic experiment result to verticillium dahliae in the present invention；
Figure 12 is the denatonium benzoate bacteriostatic experiment result to cercospora black spot of peanut bacterium in the present invention；
Figure 13 is the denatonium benzoate bacteriostatic experiment result to blue berry branch ulcer bacteria in the present invention；
Figure 14 is the denatonium benzoate bacteriostatic experiment result to Valsa mali in the present invention；
Figure 15 is the denatonium benzoate bacteriostatic experiment result to Botryosphaeria berengeriana f. sp in the present invention；
Figure 16 is the denatonium benzoate bacteriostatic experiment result to pears rotten pathogenic bacteria in the present invention；
Figure 17 is the denatonium benzoate bacteriostatic experiment result to Lignum cinnamomi camphorae phytophthora root rot bacterium in the present invention；
Figure 18 is the denatonium benzoate bacteriostatic experiment result to tobacco black shank bacterium in the present invention.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, technical scheme is described in further detail.
Embodiment 1
One, experiment material
Denatonium benzoate is the finished product bought from Sigma company.Weighing 8.92g denatonium benzoate in the ultra-pure water of 200ml, be made into the mother solution of 0.1M, filtration sterilization 4 DEG C preservation is stand-by.
Phytopathogen used in this experiment is the strain that laboratory 4 DEG C preserves, and the culture medium of employing is potato culture (being called for short PDA).
PDA culture medium prescription: Rhizoma Solani tuber osi (peeling) 200g, glucose 20g, agar 15g, tap water 1000ml, natural PH
Compound method: Rhizoma Solani tuber osi is cleaned peeling, 200g is claimed to be cut into small pieces, add water well-done (boiling 20-30 minute, can be poked by Glass rod), by eight layers of filtered through gauze in beaker, need to add 15-20g agar according to experiment, adding 20g glucose, stir, after fully dissolving, slightly cooling supplies water to 1000ml, after subpackage, 121 DEG C of sterilizings 20 minutes, standby after cooling.
Two, experimental technique
Adopt growth rate method.
1, first by stand-by for 18 kind of plant pathogen, 25 DEG C of cultivation about 6d on PDA plate.
2, the heating of PDA culture medium being dissolved, be cooled to 45-50 DEG C, the denatonium benzoate being separately added into variable concentrations makes the culture medium containing 0,5,10 and 20mM medicinal liquid, and pours cooling in culture dish respectively into.
3, with sterile working's formality, beat at each bacterial strain mycelia edge (upgrowth situation consistent) cultivating 6d with card punch and take circle bacterium cake (diameter 0.60cm) as far as possible, choose to pastille flat board central authorities with Inoculating needle again, then culture dish is inverted in incubator (25 DEG C) and cultivates.
4, the growing state of different time Observe and measure mycelia after processing, and adopt decussation method record diameter and process data, calculate suppression ratio and take pictures.
Suppression ratio (%)=(comparison hyphal diameter-process hyphal diameter)/comparison hyphal diameter × 100
Each process is repeated 3 times.
Three, the denatonium benzoate fungistatic effect to 18 kind of plant pathogen mycelial growths
1. verticillium dahliae: the pathogen causing cotton verticillium wilt is the verticillium dahliae that Verticillium belongs to.The host range of this pathogen is extremely wide, abroad reports 38 section 660 kind of plant, wherein crops 184 kinds of can causing harm, 153 kinds of weeds.China is identified, and host plant at least 20 section 80 kinds, field crop includes Cotton Gossypii, Helianthi, Fructus Solani melongenae, spicy, Fructus Lycopersici esculenti, Nicotiana tabacum L., Rhizoma Solani tuber osi, Fructus Melo, Citrullus vulgaris, Fructus Cucumidis sativi, Semen arachidis hypogaeae, Kidney bean, Semen phaseoli radiati, Semen sojae atricolor, Semen Sesami, Radix Betae etc..This pathogenic bacteria can make host plant produce verticillium wilt, worldwide causes great economic loss.Verticillium wilt preventing and treating difficulty, existing means of prevention such as Biological control, chemical prevention, conventional genetic breeding etc. are all unable to reach desirable prevention effect.
2. tobacco black shank bacterium: this pathogenic bacteria can endanger all cultivations Nicotiana tabacum L. such as flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, Turkish tobaccos, burley tobaccos, causes black shank.Reaching 22 DEG C at temperature on average, rainy time and low-lying humid area, black shank spreads quickly, whole vega can be made to destroy in 1～2 week, destructive extremely strong, often results in the whole strain death of cigarette strain after field infestation.Black shank is a kind of destructive soil-borne disease, is also one of global Main Tobacco Diseases.Once occur in production, if processed not in time, will result in serious economic loss.The host range of this pathogenic bacteria is narrower, and Nicotiana tabacum L. is its unique host under field conditions (factors).Although host range is larger under artificial inoculation conditions, but also only infect a few plant such as stem and Rhizoma Solani tuber osi, Fructus Solani melongenae, Fructus Capsici, Semen Ricini, Fructus Mali pumilae, striped calabash of tomato seedling and fruit, the cotton boll of Cotton Gossypii, papaw.
3. cercospora black spot of peanut bacterium: the cercospora black spot of peanut is commonly called as " black subcutaneous ulcer ", " black puckery ", for modal leaf portion, domestic and international Semen arachidis hypogaeae producing region fungal disease, all can occur in the Semen arachidis hypogaeae whole season of growth, but its onset peak comes across the middle and late growth stage of Semen arachidis hypogaeae more, therefore have the title of " speckle in evening " disease.Often resulting in plant to fall leaves in a large number, cause shell development and be obstructed, yield falls sharply.This pathogenic bacteria only encroaches on Semen arachidis hypogaeae, does not still find other hosts so far.Pathogenic bacteria mainly survives the winter on invalid body with Stroma, mycelia and conidium, it is also possible to ascocarp is in invalid body, or is attached on kind of a shell, seed with conidium and survives the winter, and becomes the source of morbidity in the 2nd year.The conidium that pathogenic bacteria mycelia directly produces when condition is suitable is propagated with wind and rain.The scab produced primarily occur ins on the Lao Ye of soil table, can cause nearly all leaf abscission before results.
4. tomato early blight bacterium: this disease, except directly endangering stem, leaf, fruit, may also suppress tomato growth and fruit is formed, and open country and protecting field are injured all heavier, long-term underproduction 20%-30%, up to more than 50% time serious, even have no harvest.This cause of disease host range is extensive, except harm Fructus Lycopersici esculenti, also can endanger the solanaceous vegetable crops such as Fructus Solani melongenae, Fructus Capsici and Rhizoma Solani tuber osi.
5. fusarium graminearum: head blight is a kind of destructive disease, can cause fringe rotten, causes Severe Reduction and quality to reduce.Along with the change of global warming, cropping system and mode, wheat scab constantly spreads extension, often results in wheat yield, quality reduction, and containing mycotoxin in the wheat seed infected, can cause person poultry poisoning and serious disease.This pathogenic bacteria is except harm Semen Tritici aestivi, moreover it is possible to infect the grassy weed such as the multiple gramineous crop such as Fructus Hordei Vulgaris, Herba bromi japonici, Oryza sativa L., Semen Maydis and goose hat grass, additionally, also can infect the crops such as Semen sojae atricolor, Cotton Gossypii, Radix Ipomoeae.
6. Valsa mali: apple tree canker is the trunk disease that apple tree is main, the most serious.This disease can also infect fruit except infecting branch, causes deadwood, extremely sets, and causes the garden of ruining time serious, thus causing serious yield and economic loss.Apple rot pathogen has and compares host plant widely, and harm includes the common fruit tree of Fructus Mali pumilae, Lou Tao, Fructus Persicae and pears etc..
7. potato wilt bacterium: Fusarium oxysporum is the Main Pathogenic Bacteria of potato wilt, and this bacterium is a kind of universal soil-borne disease fungal pathogens, host range is very extensive, can cause the generation of the various plants droops such as Solanaceae, melon, Fructus Musae, pulse family, cotton and flowers.After host is infected by Fusarium oxysporum, Symptoms is varied, generally results in vascular bundle brown stain, and plant is wilted withered, bulb and root rot, and plant strain growth is weak.Potato wilt is a kind of important fungoid soil-borne disease in recent years Rhizoma Solani tuber osi worked the mischief, in the existing generation in a lot of areas, especially even more serious at Rhizoma Solani tuber osi weight weak ground occurrence degree, directly affects potato yield and economic benefit thereof.
8. blue berry branch-rot bacterium: this pathogenic bacteria belongs to plan Pestalotia, is a kind of important phytopathogen, can the plant of parasitic about section more than 50, show as tikka after hazard of plant, rot, the symptom such as ulcer, have a strong impact on the yield and quality of product.Therefore, the production of farm-forestry crop is had important economic implications by research plan pestalotia bacteria.At present, the blue berry main breed in production, Ru Lanfeng, duke and blue beauty etc., all there is branch-rot to occur, its fruit yield of appreciable impact and quality.
9. tobacco brown spot pathogen: Alternaria alternate is the fungal disease of harm China tobacco leaf production, can not only make quality of tobacco decline and blade incompleteness is damaged, moreover it is possible to reducing its industrial application and be worth, susceptible Nicotiana tabacum L. makes the mouthfeel of Nicotiana tabacum L. decline owing to tobacco leaf ingredient differs.Alternaria altemata host range is relatively wide, outside smoke abatement grass, also can infect the various plants such as Cotton Gossypii, Semen arachidis hypogaeae, Semen sojae atricolor, Fructus Lycopersici esculenti, Fructus Persicae, Lee, Semen Tritici aestivi, cause the symptom such as speckle, root rot.
10. Fructus Cucumidis sativi rod spore leaf spot fungi: this pathogenic bacteria host range is extensive, and circulation way is various.Further investigation reveals that in recent years, the leaf spot of the vegetables such as the Fructus Cucumidis sativi rod microbial Fructus Cucumidis sativi of spore leaf spot, Fructus Lycopersici esculenti occur in 11 province, city and region's large area such as the Shandong of China, Hebei, Liaoning, the Inner Mongol, cause serious economic loss.
11. blue berry intends stem point branch-rot bacterium: it is a kind of important trunk disease on blue berry that blue berry intends stem point branch-rot.Host range is relatively wide, mainly has the plants such as Larch, Fructus Persicae, pear, Vaccinium, Jianshi maple.
12. Botryosphaeria berengeriana f. sp: it is sick that ring rot of apple has another name called scurf, wheel stricture of vagina decayed fruit, is the important biomolecule disaster on Fructus Mali pumilae branch portion and fruit, often cause that Fructus Mali pumilae branch bark is coarse, locality is downright bad and fruit rot.Ill plant fruit-setting rate is low, causes tree asthenia weak and yield reduces, and even garden is ruined in total crop failure.In recent years, along with the commerial growing of susceptible kind Fuji apple, ring rot of apple sickness rate increases year by year, and hazard area constantly expands, it has also become the serious plant disease in Chinese apple production, the sustainable development of Apple Industry in serious threat.
13. pears rotten pathogenic bacteria: pears canker has another name called bark rot, the major branch of main harm pear tree and side shoot, causing that pear tree gesture is weak, fruit yield and quality decline.This disease has that generation area is wide, sickness rate is high, unmanageable feature.All there is generation in China's pears main producing region, especially occur with Xinjiang, northwest, North China, northeast and other places serious.Falling ill serious theatre, body disease scar is innumerable, branch is incomplete for tree, causes a large amount of Si Shuhuohui garden.
14. blue berry branch ulcer bacteria: the pathogen Botryosphaeria causing blue berry branch Peptic Ulcers is a universal fungus of class, the Tree resistance caused is the great disease occurred in world wide.The host of its harm is very wide, reach 45 genus (broad leaf tree 40 genus, 5, coniferous tree genus), the harm of woods fruit tree is serious, the symptoms such as the branch ulcer withered death of the host plants such as Eucalyptus, willow, Fructus Mali pumilae, Punica granatum L., peach tree, Semen Cassiae, Fructus Myricae rubrae, Semen Castaneae, dry rot, gummosis can be caused.
15. botrytis cinerea: graw mold of tomato is that the pathogen of Botrytis cinerea infects caused Common Diseases, it is endanger one of maximum disease, occurs general.Except harm Fructus Lycopersici esculenti, also can endanger 20 various crop such as harmful Fructus Solani melongenae, Fructus Capsici, Fructus Cucumidis sativi, calabash courd.The time harm that low temperature, continuous rainy weather are many is serious.Cause the withered and substantial amounts of etching of stem and leaf, decayed fruit when falling ill serious, directly affect yield.This pathogenic bacteria is the facultative parasite that a kind of host range is very wide, can infect various fruits, vegetable and flowers.
16. Lignum cinnamomi camphorae phytophthora root rot bacterium: mould, the Cortex Cinnamomi epidemic disease poison mould also known as brown epidemic disease of Camphor tree epidemic disease, cinnamon butt rot and branch ulcer etc. can be caused, cause extreme loss.In China, Camphor tree epidemic disease is mould except the phytophthora root rot causing cinnamon, it is also possible to cause Cedrus deoclar (Roxb.) G. Don epidemic disease rot.This pathogenic bacteria is distributed widely in the areas such as America, Europe and East Asia, South Asia and Oceania, can infect include forest and crops more than 3000 various plants, be one of the important pathogen of forest disease control.
17. Maize Curvularia: Maize Curvularia Leaf Spot Disease, also known as plan eye spot, black points, maize leaf of mainly causing harm, also endangers sheath and bract sometimes, and its symptom variation is very big in different cultivars.This pathogenic bacteria host range is wider, it is possible to parasitize on the fruit of Fructus Lycopersici esculenti, Fructus Capsici, it is also possible to colonize on Oryza sativa L., Sorghum vulgare Pers., Semen Tritici aestivi and some lawns so that these plant generation diseases.Maize Curvularia Leaf Spot Disease is a kind of disease that China North China, northeast corn producing region generally occur in recent years, owing to this disease is sudden disease, spread rapidly, time serious, leaf portion scab is intensive in flakes, severe disease plot diseased plant rate and sick leaf rate are up to 100%, it has also become one of the important disease in China's Semen Maydis producing region.
18. Tobacco target spot bacterium: Tobacco target spot is by the microbial tikka class disease of miliary damping-off, this pathogenic bacteria main harm blade forms scab, and scab necrosis part is frangible forms perforation, and when occurring serious, scab is in flakes, and plant disease epidemic speed, therefore constitutes serious threat to tobacco leaf production.The host range of this pathogenic bacteria is extensive, is possible not only to infect Nicotiana tabacum L., can infect 200 various plants in natural situation, cereal crops and Radix Betae, Fructus Solani melongenae, Fructus Lycopersici esculenti, Herba Solani Nigri, honor fiber crops and the petunia etc. such as including Semen Maydis, Sorghum vulgare Pers., Cotton Gossypii, wheat and barley, Oryza sativa L., Semen sojae atricolor.Additionally, this pathogenic bacteria can infect 188 kind of plant of 32 sections under artificial inoculation conditions.
The in vitro bacteriostasis life of 18 kind of plant pathogen is surveyed result in Table 1 by denatonium benzoate.
Table 1 denatonium benzoate inhibitory action measurement result (6d) to 18 kinds of pathogen mycelial growths
Note: in test, each process sets three repetitions, in table, data are the meansigma methods repeated for three times.
As can be known from Table 1, along with the rising of denatonium benzoate concentration, the inhibition of 18 kind of plant pathogen is also had raising in various degree by it.When denatonium benzoate concentration is 10mM, 18 kind of plant pathogen of Deuteromycotina, Ascomycotina and Mastigomycotina are all had good inhibition by it, suppression ratio is all more than 85%, wherein to the suppression ratio of the 9 kind of plant pathogen such as botrytis cinerea, blue berry branch ulcer bacteria and Botryosphaeria berengeriana f. sp more than 90%.Especially the fungistatic effect of 7 kinds of pathogen such as Lignum cinnamomi camphorae phytophthora root rot bacterium, Valsa mali and pears rotten pathogenic bacteria is best, it, all more than 99%, can effectively be prevented and treated by its suppression ratio.
Denatonium benzoate is to the fungistatic effect picture of 18 kind of plant pathogen mycelial growths such as shown in Fig. 1-18, and when the concentration of denatonium benzoate is when 10mM, 7 kinds of pathogen such as Lignum cinnamomi camphorae phytophthora root rot bacterium, Valsa mali, tobacco black shank bacterium are almost without mycelial growth.And for the 8 kind of plant pathogen such as botrytis cinerea, blue berry branch ulcer bacteria and Botryosphaeria berengeriana f. sp, only little mycelial growth.Additionally, for tobacco brown spot pathogen, cercospora black spot of peanut bacterium and Tobacco target spot bacterium, though there being mycelial growth, but compared with the control, mycelial growth is more weak, illustrate that denatonium benzoate serves good inhibition.In sum, in the very potential preventing and treating being applied to phytopathogen of denatonium benzoate.
Above example is only in order to illustrate technical scheme, but not is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent replacement；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of present invention technical scheme required for protection.
Claims (8)
1. denatonium benzoate is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic.
2. denatonium benzoate according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described phytopathogen is Deuteromycotina fungus, Ascomycotina fungus and Mastigomycotina fungus.
3. denatonium benzoate according to claim 1 and 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: in described antibacterial, effective working concentration of denatonium benzoate is 5mM-10mM.
4. denatonium benzoate according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Deuteromycotina fungus includes blue berry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blue berry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, Fructus Cucumidis sativi rod spore leaf spot fungi, verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium, cercospora black spot of peanut bacterium and Maize Curvularia.
5. denatonium benzoate according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Ascomycotina fungus includes blue berry branch ulcer bacteria, Botryosphaeria berengeriana f. sp, Valsa mali and pears rotten pathogenic bacteria.
6. denatonium benzoate according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Mastigomycotina fungus includes Lignum cinnamomi camphorae phytophthora root rot bacterium and tobacco black shank bacterium.
7. denatonium benzoate according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterized in that: when in described antibacterial, the concentration of denatonium benzoate is 10mM, denatonium benzoate to the suppression ratio of the 7 kind of plant pathogen such as botrytis cinerea, blue berry branch ulcer bacteria and Botryosphaeria berengeriana f. sp up to 99%.
8. denatonium benzoate according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described plant is Lignum cinnamomi camphorae, Fructus Mali pumilae, Fructus Persicae, pears, Fructus Lycopersici esculenti, blue berry, Fructus Vitis viniferae, Nicotiana tabacum L., Fructus Cucumidis sativi, Cotton Gossypii, Oryza sativa L., Semen Maydis, Semen Tritici aestivi, Semen arachidis hypogaeae and Semen sojae atricolor.
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|---|---|---|---|---|
| BE1028640B1 (en) * | 2020-09-28 | 2022-04-25 | Sentinalco | Process for manufacturing a denatonium benzoate concentrate in liquid form and the installations required for said manufacturing process without risk of gas/dust explosion |
| CN117678601A (en) * | 2023-12-08 | 2024-03-12 | 秦皇岛禾苗生物技术有限公司 | Application of benidiammonium in preventing and controlling plant bacterial diseases |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101553205A (en) * | 2006-10-17 | 2009-10-07 | 拉伯泰克技术研发有限公司 | Adhesive label with bittering agent and fluidifying agents for natural airway secretions |
| US8492312B2 (en) * | 2005-11-10 | 2013-07-23 | Regents Of The University Of Minnestoa | Systemic plant conditioning composition |
-
2016
- 2016-03-30 CN CN201610191166.5A patent/CN105746516B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN101553205A (en) * | 2006-10-17 | 2009-10-07 | 拉伯泰克技术研发有限公司 | Adhesive label with bittering agent and fluidifying agents for natural airway secretions |
Non-Patent Citations (1)
| Title |
|---|
| T.F. KENNEDY等: "An investigation of seed treatments for the control ofcrow damage to newly-sown wheat", 《IRISH JOURNAL OF AGRICULTURAL AND FOOD RESEARCH》 * |
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|---|---|---|---|---|
| BE1028640B1 (en) * | 2020-09-28 | 2022-04-25 | Sentinalco | Process for manufacturing a denatonium benzoate concentrate in liquid form and the installations required for said manufacturing process without risk of gas/dust explosion |
| CN117678601A (en) * | 2023-12-08 | 2024-03-12 | 秦皇岛禾苗生物技术有限公司 | Application of benidiammonium in preventing and controlling plant bacterial diseases |
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