CN105766906B - Curcumin is being prepared for preventing by the purposes in the fungicide of the microbial plant disease of pathogenic - Google Patents
Curcumin is being prepared for preventing by the purposes in the fungicide of the microbial plant disease of pathogenic Download PDFInfo
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Abstract
The present invention provides curcumin Toxicity Determination passed through by the purposes in the fungicide of the microbial plant disease of pathogenic, the present invention preparing to be used to prevent, it was demonstrated that curcumin has good inhibitory activity to plant pathogenic fungi.Curcumin has the advantages that efficient and low toxicity, is suitable for the requirement of plant disease chemical prevention as fungicide.The use of bulk sterilization agent at present, causes the resistance to the action of a drug of pathogen to strengthen, and traditional fungicide causes serious pollution to the environment, remains height, directly threatens the food security of the mankind.And curcumin is a kind of degradable, pollution-free, environment amenable micromolecular compound, and its resistance to the action of a drug is poor, to non-target organism and safety of human and livestock, it can ensure the high-quality of agricultural product and fruits and vegetables, meet the requirement of sustainable development, it is studied and market application foreground is wide.
Description
Technical field
The present invention relates to tool Fungicidal active compound, more particularly to curcumin is used to prevent and is drawn by phytopathogen in preparation
Purposes in the fungicide of the plant disease risen.
Background technology
Since 21 century, with scientific and technological progress and the raising of social productive forces, the mankind create unprecedented wealth, with
This at the same time, the problems such as population increase, shortage of resources, environmental pollution, ecological disruption and frequent natural calamity, becomes increasingly conspicuous, serious resistance
The sustainable development of agricultural is hindered, therefore agricultural production also undertakes unprecedented immense pressure.According to FAO (Food and Agriculture Organization of the United Nation)
Estimation, global grain and Cotton Production are lost more than 10% throughout the year because of disease.Plant disease can not only cause crops
The reduction of yield, and quality safety and its international trade of the serious threat to agricultural product are gone back to a certain extent.
The history of plant disease chemical prevention develops with the generation of plant disease, the practice and application of chemical prevention
Has the history in century more than one so far.Chemical prevention have the advantages that quick, wide sterilization spectrum, cost it is low, using simplicity, but
Long-term a large amount of uses of fungicide can cause the environmental pollutions such as soil, air, destroy the ecological balance.As people in recent years are to food
The concern of product safety and problem of environmental pollution, some chemical bactericides have been strictly limited in many developed countries and regions to be made
With.People begin look for it is a kind of to the mankind and it is environmentally friendly and with good prevention effect new control of plant disease strategy.
Novel green fungicide is efficient and nontoxic, it is harmless, pollution-free, do not develop immunity to drugs, not only conform with need of the people to pollution-free food
Ask, and the sustainable development for agricultural provides guarantee, it is therefore desirable to have more efficient, low toxicities, low-residual and environment friend
The fungicide of good type is developed and applies, and plays an important role in agricultural production.
Curcumin, also known as turmeric；Chemical name：(E, E) -1,7- double (4- hydroxy 3-methoxybenzenes base) -1,6- heptan two
Alkene -3,5- diketone；English name：curcumin、6-Heptadiene-3,5-dione,1,7-bis(4-hydroxy-3-
methoxyphenyl)-,(E,E)-1、turmeric yellow.No. CAS：458-37-7, molecular formula：C21H20O6, molecular weight
368.38 molecular structural formula is：
Curcumin is in orange-yellow crystalline powder.183 DEG C of fusing point.It is not soluble in water, it is slightly soluble in ether and benzene, when heating can be dissolved in
Ethanol, is soluble in glacial acetic acid and aqueous slkali.It is fast light, heat resistance is poor.PH value is in yellow when being 7.8, in red when pH value is 9.2
Color.By being obtained in the root of turmeric.Plant origin has the root tuber of zingiberaceous plant Radix Curcumae (Curcuma aromatica Salisb), ginger
Yellow (C.longa L.) rhizome, curcuma zedoary (C.zedoaria (Berg.) Rosc.) rhizome etc., can also chemical synthesis and biology conjunction
Into.Reacted and obtained by vanillic aldehyde and acetylacetone,2,4-pentanedione in production.Main method：Vanillic aldehyde, Ran Houjia are dissolved with anhydrous ethyl acetate
Enter butyl borate and the complex compound generated by acetylacetone,2,4-pentanedione, B2O3, then n-butylamine is added dropwise, drip off stirring 4-5h, stand overnight.
Next day adds 60 DEG C of 0.4N hydrochloric acid and continues to stir 1h, and with 50 DEG C of water-baths insulations, makes that the reaction was complete.Divide dereaction product
Water layer, is washed with water 3-4 times, filters out curcumin, and washing 2-3 times with ethyl acetate obtains crude product, and finished product is obtained with ethyl alcohol recrystallization.
Curcumin is a kind of natural dye, can directly make dyestuff use, it is also possible to makees pH indicator, color change interval 7.8
~9.2, bronzing is become by yellow.In addition, curcumin have cholagogic, antibacterial, anti-oxidant, anti-nitrosation, carcinogenesis, anti-mutagenesis,
The effects such as anti-liver is malicious, anti-infective, antiviral and anticoagulation.It is alternatively arranged as food flavor, it is preservative, colouring agent, cosmetics, strong
Stomach agent, anodyne, diuretics, cholagog, are used for turmeric test paper processed, pH value 8~9, it can also be used to which boric acid is determined on chemical industry
Property analysis.
At present, it there is no the report to agriculture pathogen inhibitory action on curcumin both at home and abroad.
The content of the invention
Object of the present invention is to provide curcumin to prepare for preventing by the microbial plant disease of pathogenic
Fungicide in purposes.The present invention passes through toxicity test, it was demonstrated that curcumin to plant pathogenic fungi has good suppression
Activity.For curcumin as fungicide, its efficient, low toxicity, is suitable for the requirement of plant disease chemical prevention.
For achieving the above object, the present invention is achieved by the following scheme：
The present invention provides curcumin to prepare for preventing in the fungicide by the microbial plant disease of pathogenic
Purposes.
Further：The phytopathogen is Deuteromycotina fungi, Ascomycotina fungi and Mastigomycotina are true
Bacterium.
Further：Effective concentration of curcumin is 15mM-30mM in the fungicide.
Further：The Deuteromycotina fungi includes blueberry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato morning
Epidemic disease bacterium, blueberry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, cucumber rod spore leaf spot fungi,
Verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium and Curvularia
Leaf spot fungi.
Further：The Ascomycotina fungi includes apple anthrax leaf spoting bacteria, blueberry limb ulcer bacteria, apple
Target spot pathogen, Valsa mali and pears rotten pathogenic bacteria.
Further：The Mastigomycotina fungi includes pathogen of soybean root rot and tobacco black shank bacterium.
Further：When the concentration of curcumin is 30mM in the fungicide, curcumin is rotten to Botrytis cinerea, apple
The inhibiting rate of 9 kinds of phytopathogens such as rotten germ and tomato early blight bacterium is up to 99%.
Further：The plant is apple, peach, pears, tomato, blueberry, grape, tobacco, cucumber, cotton, rice, jade
Rice, wheat and soybean.
Compared with prior art, advantages of the present invention and have the technical effect that：The kind mechanism of action of fungicide compares at present
Single, the reproduction speed of pathogen is very fast, therefore resistance also produces comparatively fast, and pollutes big, high residue, threatens the food peace of the mankind
Entirely.
The present invention passes through Toxicity Determination, it was demonstrated that curcumin to plant pathogenic fungi has good inhibitory activity.
Curcumin has the advantages that efficient and low toxicity, is suitable for the requirement of plant disease chemical prevention as fungicide.Largely kill at present
The use of microbial inoculum, causes the resistance to the action of a drug of pathogen to strengthen, and traditional fungicide causes serious pollution to the environment, remains height, direct prestige
Coerce the food security of the mankind.As people increasingly improve pollution-free, pollution-free green food cry, green bactericide is
As the important control method in agricultural production, many green bactericides have been come out one after another, and are widely used in producing, and are achieved
Remarkable result.And curcumin is a kind of degradable, pollution-free, environment amenable micromolecular compound, and its resistance to the action of a drug it is poor,
To non-target organism and safety of human and livestock, it can ensure the high-quality of agricultural product and fruits and vegetables, meet the requirement of sustainable development, it grinds
Study carefully wide with market application foreground.
Brief description of the drawings
Fig. 1 be in the present invention curcumin to the bacteriostatic experiment result of Valsa mali；
Fig. 2 be in the present invention curcumin to the bacteriostatic experiment result of tobacco brown spot pathogen；
Fig. 3 be in the present invention curcumin to the bacteriostatic experiment result of Botrytis cinerea；
Fig. 4 be in the present invention curcumin to the bacteriostatic experiment result of tomato early blight bacterium；
Fig. 5 be in the present invention curcumin to the bacteriostatic experiment result of fusarium graminearum；
Fig. 6 be in the present invention curcumin to the bacteriostatic experiment result of pathogen of soybean root rot；
Fig. 7 be in the present invention curcumin to the bacteriostatic experiment result of tomato wilt bacterium；
Fig. 8 be in the present invention curcumin to the bacteriostatic experiment result of pears rotten pathogenic bacteria；
Fig. 9 be in the present invention curcumin to the bacteriostatic experiment result of Tobacco target spot bacterium；
Figure 10 is the bacteriostatic experiment result that curcumin intends blueberry stem point branch-rot bacterium in the present invention；
Figure 11 be in the present invention curcumin to the bacteriostatic experiment result of tobacco black shank bacterium；
Figure 12 be in the present invention curcumin to the bacteriostatic experiment result of blueberry branch-rot bacterium；
Figure 13 be in the present invention curcumin to the bacteriostatic experiment result of potato wilt bacterium；
Figure 14 be in the present invention curcumin to the bacteriostatic experiment result of Botryosphaeria berengeriana f. sp；
Figure 15 be in the present invention curcumin to the bacteriostatic experiment result of rice blast fungus；
Figure 16 be in the present invention curcumin to the bacteriostatic experiment result of Ustilaginoidea virens；
Figure 17 be in the present invention curcumin to the bacteriostatic experiment result of blueberry limb ulcer bacteria；
Figure 18 be in the present invention curcumin to the bacteriostatic experiment result of cucumber rod spore leaf spot fungi；
Figure 19 be in the present invention curcumin to the bacteriostatic experiment result of verticillium dahliae；
Figure 20 be in the present invention curcumin to the bacteriostatic experiment result of Maize Curvularia；
Figure 21 be in the present invention curcumin to the bacteriostatic experiment result of apple anthrax leaf spoting bacteria；
Embodiment
Technical scheme is described in further detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
First, experiment material
Curcumin is the finished product from the purchase of Sigma companies.7.37g curcumins are weighed in the dimethyl sulfoxide (DMSO) of 20ml, are matched somebody with somebody
Into the mother liquor of 1M, 4 DEG C of preservations of filtration sterilization are stand-by.
Phytopathogen used is the strain of the 4 DEG C of preservations in laboratory in this experiment, and the culture medium used is potato training
Support base (abbreviation PDA).
PDA culture medium formula：Potato (peeling) 200g, glucose 20g, agar 15g, tap water 1000ml, natural PH
Preparation method：Potato is cleaned into peeling, claims 200g to be cut into small pieces, adds boiling is rotten (to boil 20-30 minutes, energy quilt
Glass bar is poked), with eight layers of filtered through gauze in beaker, needed to add 15-20g agar according to experiment, add 20g grapes
Sugar, stirs evenly, and fully slightly cooling supplies water to 1000ml after dissolving, sterilizes 20 minutes for 121 DEG C after packing, spare after cooling.
2nd, experimental method
Using growth rate method.
1st, first by 21 kinds of phytopathogens, 25 DEG C of culture 2d or so are stand-by on PDA plate.
2nd, PDA culture medium heating is dissolved, is cooled to 45-50 DEG C, the curcumin for being separately added into various concentrations is made containing 0,
7.5th, the culture medium (dimethyl sulfoxide (DMSO) that respective volume is added in control) of 15 and 30mM liquids, and pour into respectively cold in culture dish
But.
3rd, with sterile working formality, beaten with card punch at each bacterial strain mycelia edge (upgrowth situation is as far as possible consistent) of culture 2d
Circular bacteria cake (diameter 0.60cm) is taken, then is chosen with transfer needle to drug containing tablet center, culture dish is then inverted in incubator (25
DEG C) in culture.
4th, after processing different time Observe and measure mycelia growing state, and measure diameter and locate using crossing method
Data are managed, inhibiting rate is calculated and takes pictures.
Inhibiting rate (%)=(control hyphal diameter-processing hyphal diameter)/control hyphal diameter × 100
Each processing is repeated 3 times.
3rd, fungistatic effect of the curcumin to 21 kinds of phytopathogen mycelial growths
1. verticillium dahliae：The pathogen for causing cotton verticillium wilt is the verticillium dahliae that Verticillium dahliae belongs to.The pathogen
Host range it is extremely wide, foreign countries' report can cause harm 38 section, 660 kinds of plants, wherein 184 kinds of crops, 153 kinds of weeds.China is through mirror
Fixed, host plant at least 80 kinds of 20 section, field crop includes cotton, sunflower, eggplant, spicy, tomato, tobacco, potato, sweet tea
Melon, watermelon, cucumber, peanut, Kidney bean, mung bean, soybean, sesame, beet etc..The germ can make host plant produce verticillium wilt,
Great economic loss is caused in world wide.Verticillium wilt prevention is difficult, and such as biological control of existing means of prevention, chemistry are anti-
Control, conventional genetic breeding etc. is all unable to reach preferable prevention effect.
2. tobacco black shank bacterium：The germ can endanger all cultivation cigarettes such as flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, Turkish tobaccos, burley tobaccos
Grass, causes tobacco black shank.In temperature on average up to 22 DEG C, rainy time and low-lying humid area, tobacco black shank are spread
Quickly, can destroy in 1~2 week whole vega, it is destructive extremely strong, it is dead that the whole strain of cigarette strain is often resulted in after field infestation.Tobacco is black
Shin disease is a kind of destructive soil-borne disease, and one of global Main Tobacco Diseases.Once occurring, if not in production
Timely processing, will result in serious economic loss.The host range of the germ is relatively narrow, tobacco be its under field conditions (factors) only
One host.Although host range is larger under artificial inoculation conditions, tomato seedling and fruit, the cotton of cotton are also only infected
A few plant such as bell, the stem of papaw and potato, eggplant, capsicum, castor-oil plant, apple, striped cucurbit.
3. pathogen of soybean root rot：Soybean phytophthora root rot is a kind of by the microbial disease of soybean phytophthora, although it
Origin relatively late, but it is that serious phytophthora blight is uniquely endangered in field crop.Its hazard area is big, degree weight,
Oneself is by one of row soybean destructive disease, and the extremely strong survival ability of characteristic and its pathogen for being passed due to its soil, the disease is so far
In the world, major soybean production areas is still in the gesture to expand.The germ host range is narrow, only infects soybean.Can be in all lifes of soybean
The phase of educating infects soybean.Seriously ill, disease short incubation period usually is issued in moist rainy weather condition, then is infected often, is propagated
Rapid with spreading, host is dead in flakes, seldom single plant morbidity.
4. tomato early blight bacterium：The disease may also suppress tomato growth and fruit formed in addition to stem, leaf, fruit is directly endangered, dew
Ground and protecting field are aggrieved all heavier, long-term underproduction 20%-30%, up to more than 50%, or even total crop failure when serious.This cause of disease host
It is in extensive range, in addition to tomato is endangered, it can also endanger the solanaceous vegetable crop such as eggplant, capsicum and potato.
5. rice blast fungus：Rice blast is a kind of disease as caused by rice blast fungus, is rice important in the world
One of disease, same to banded sclerotial blight, bacterial leaf-blight are listed in three major disease of rice.The rice underproduction 10% -20% is typically resulted in, seriously
When can the underproduction 40% -50%, or even total crop failure.The germ not only infects rice, moreover it is possible to the crops such as wheat, barley and grain are infected,
The root of plant is even infected, serious harm is caused to agricultural production.
6. fusarium graminearum：Head blight is a kind of destructive disease, and fringe can be caused rotten, causes Severe Reduction and quality to drop
It is low.With the change of global warming, cropping system and mode, wheat scab constantly spreads extension, often results in wheat
The underproduction, quality reduce, and contain mycotoxin in the wheat seed infected, can cause person poultry poisoning and serious disease.The disease
Bacterium is in addition to wheat is endangered, moreover it is possible to infects the grass family such as a variety of gramineous crops such as barley, oat, rice, corn and goose hat grass
Weeds, in addition, can also infect the crops such as soybean, cotton, sweet potato.
7. tomato wilt bacterium：The specialization of tomato wilt bacterium is extremely strong, only endangers tomato.The bacterium mainly infects plant and leads
Tubing, causes plant wilt by blocking xylem material and Water Transportation.It is a kind of serious harm quality of vegetable and production
The soil-borne disease of amount, the Seedling Stage of tomato during Adult plant be subject to wilt to being infected.Tomato wilt, which is called, withers
Listless disease, occurs serious in old dish canopy room.
8. Valsa mali:Apple tree canker is that apple tree is main, trunk disease of most serious.The disease is except invading
Fruit can also be infected outside dye limb, deadwood, dead tree is caused, causes to ruin garden when serious, so as to cause serious yield and economy
Loss.Apple rot pathogen possesses the extensive host plant of comparison, and harm is normal including apple, Lou Tao, peach and pears etc.
See fruit tree.
9. potato wilt bacterium：Fusarium oxysporum is the Main Pathogenic Bacteria of potato wilt, and the bacterium is a kind of
Universal soil-borne disease fungal pathogens, host range is quite varied, can cause Solanaceae, melon, banana, pulse family, cotton and flowers
Etc. the generation of various plants droop.After host is infected by Fusarium oxysporum, Symptoms are varied, generally result in vascular bundle
Brown stain, plant are wilted withered, bulb and root rot, and plant strain growth is weak etc..Potato wilt is that potato is made in recent years
Into a kind of important fungoid soil-borne disease of harm, occurred in many areas, especially potato weight it is weak journey occurs
Spend even more serious, directly affects potato yield and its economic benefit.
10. apple anthrax leaf spoting bacteria：Apple anthrax leaf blight is a kind of leaf diseases as caused by anthrax bacteria, mainly
The kinds such as loud, high-pitched sound drawing, Jin Guan, Qiao Najin, Qin Guan of apple are endangered, causes apple tree largely to fall leaves and seriously rots with fruit, tree vigo(u)r is tight
Weaken again, cause next year to be had no harvest.Fuji, red magnitude kind are highly disease-resistant, and the loud, high-pitched sound of morbidity is drawn and the adjacent Fuji apple not fallen ill
Tree contrasts clearly in orchard.Apple anthrax-bacilus leaf blight main harm blade, and cause largely to fall leaves.
11. blueberry branch-rot bacterium：The germ belongs to plan Pestalotia, is a kind of important phytopathogen, can be parasitic
The plant of section about more than 50, tikka is shown as after hazard of plant, is rotted, the symptom such as ulcer, seriously affect product yield and
Quality.Therefore, production of the pestalotia bacteria to farm-forestry crop is intended in research has important economic implications.At present, the indigo plant in production
Certain kind of berries main breed, Ru Lanfeng, duke and blue beauty etc., has branch-rot, significantly affects its fruit yield and quality.
12. tobacco brown spot pathogen：Alternaria alternate is the fungal disease for endangering China's tobacco leaf production, can not only make tobacco leaf
Quality, which declines, and blade is incomplete damages, moreover it is possible to reduces its industrial application value, susceptible tobacco leaf makes cigarette since tobacco leaf ingredient differs
The mouthfeel of grass declines.Alternaria altemata host range is wider, in addition to tobacco, can also infect cotton, peanut, soybean, tomato, peach,
The various plants such as Lee, wheat, cause the symptoms such as spot, root rot.
13. Ustilaginoidea virens：Rice green smut is also known as pseudo- smut, green smut, paddy blossom disease, blue or green powder disease, is commonly called as " rich
Produce fruit ", it is that a kind of rice fungus diseases that Rice Panicle generation is infected by the green pyrenomycetes of rice or rice ergot are done harm to, in the world, main rice region is equal
There is generation.This disease not only causes production loss, but also seriously affects rice quality, oneself, which becomes, influences China's Rice Production and rice
One of important disease of quality.
14. cucumber rod spore leaf spot fungi：The germ host range is extensive, and circulation way is various.It further investigation reveals that in recent years,
The leaf spot of the vegetables such as cucumber, tomato as caused by cucumber rod spore leaf spot fungi is in the Shandong of China, Hebei, Liaoning, the Inner Mongol
Occur Deng 11 province, city and region's large area, cause serious economic loss.
15. blueberry intends stem point branch-rot bacterium：It is a kind of important trunk disease on blueberry that blueberry, which intends stem point branch-rot,.Host
Scope is wider, mainly there is the plants such as Larch, peach, pear, Vaccinium, Jianshi maple.
16. Botryosphaeria berengeriana f. sp：Ring rot of apple also known as scurf, wheel line decayed fruit disease, are on apple limb portion and fruit
Important biomolecule disaster, often cause that apple limb bark is coarse, locality is downright bad and fruit rot.Diseased plant fruit-setting rate is low,
Cause tree body weak and yield is reduced, or even garden is ruined in total crop failure.In recent years, with the commerial growing of susceptible kind Fuji apple,
Ring rot of apple incidence increases year by year, and hazard area constantly expands, it has also become the serious plant disease in Chinese apple production, seriously
Threaten the sustainable development of Apple Industry.
17. pears rotten pathogenic bacteria：Pears rot disease also known as bark rot, the major branch and side shoot of main harm pear tree, cause pear tree gesture
It is weak, fruit yield and quality decline.The disease has the characteristics that generation area is wide, incidence is high, unmanageable.In China pears master
There is generation in producing region, is occurred especially with Xinjiang, northwest, North China, northeast and other places serious.Fall ill serious theatre, tree body disease scar is innumerable,
Limb is incomplete, causes a large amount of Si Shuhuohui gardens.
18. blueberry limb ulcer bacteria：Pathogen-the Botryosphaeria for causing blueberry limb canker is a kind of generation
The fungi of criticality distribution, caused Tree resistance are the great diseases occurred in world wide.The host that it is endangered is very wide,
Belong to (broad leaf tree 40 belongs to, 5 categories of coniferous tree) up to 45, woods fruit tree is endangered seriously, eucalyptus, willow, apple, stone can be caused
The symptoms such as the limb ulcer withered deaths of the host plants such as pomegranate, peach, Cassia occidentalis, red bayberry, Chinese chestnut, dry rot, gummosis.
19. Botrytis cinerea：Grape grey mould is the Common Diseases in the microbial grape production of Botrytis cinerea, and
One of maximum disease is endangered, is occurred universal.The disease occurs particularly in southern region of China and northern protecting field grape production
Seriously, oneself becomes the Major Diseases of limitation northern China facility culture grapes industry development.Ruining in the disease or storage
Venereal disease of going out is done harm to, yield and quality is influenced it is very big, every year caused by gray mold grape post-harvest loss 20% -30% or so,
Cause huge economic loss.The germ is a kind of very wide facultative parasite of host range, can infect various fruits, vegetables
And flowers.
20. Maize Curvularia：Maize Curvularia Leaf Spot Disease also known as intends eye spot, black points, and mainly cause harm maize leaves
Piece, also endangers leaf sheath and bract sometimes, and the change of its symptom is very big in different cultivars.The germ host range is wider, can post
It is born on the fruit of tomato, capsicum, can also colonizes on rice, sorghum, wheat and some lawns so that these plants occurs
Disease.Maize Curvularia Leaf Spot Disease is a kind of disease that China North China, northeast corn producing region generally occur in recent years, since the disease is
Sudden disease, occurs sprawling rapidly, and leaf portion scab is intensive in flakes when serious, and grave illness plot diseased plant rate and sick leaf rate are up to
100%, it has also become one of the important disease in China corn producing region.
21. Tobacco target spot bacterium：Tobacco target spot is mainly endangered by the microbial tikka class disease of miliary damping-off, the germ
Evil blade formed scab, scab necrosis part it is frangible formed perforation, when occurring serious scab in flakes, and plant disease epidemic speed compared with
It hurry up, therefore serious threat constituted to tobacco leaf production.The host range of the germ is extensive, tobacco can be not only infected, in nature
In the case of can infect 200 various plants, including the cereal crops such as corn, sorghum, cotton, wheat class, rice, soybean and beet, eggplant
Son, tomato, black nightshade, honor fiber crops and petunia etc..In addition, the germ can infect 188 kinds of plants of 32 sections under artificial inoculation conditions
Thing.
In vitro bacteriostasis life survey of the curcumin to 21 kinds of phytopathogens the results are shown in Table 1.
Inhibitory action measurement result (6d) of 1 curcumin of table to 21 kinds of pathogen mycelial growths
Note：Each processing is set in experiment repeats three times, and data are average value three times repeatedly in table.
As can be known from Table 1, as the rise of curcumin concentration, its inhibition to 21 kinds of phytopathogens also carry successively
It is high.When curcumin concentration is 30mM, its 21 to Deuteromycotina, Ascomycotina and Mastigomycotina kind phytopathogen
Have good inhibition, inhibiting rate more than 85%, wherein to Maize Curvularia, potato wilt bacterium and
The inhibiting rate of 17 kinds of phytopathogens such as Botryosphaeria berengeriana f. sp is more than 90%.Especially to Botrytis cinerea, canker of apple fruit
The fungistatic effect of 9 kinds of pathogens such as bacterium and soybean scab bacterium is best, its inhibiting rate can carry out it effectively more than 99%
Prevention.
Curcumin to the fungistatic effect picture of 21 kinds of phytopathogen mycelial growths as shown in Fig. 1-2 1, it is dense when curcumin
In 30mM, 9 kinds of pathogens such as Botrytis cinerea, Valsa mali and soybean scab bacterium give birth to degree almost without mycelia
It is long.And for 8 kinds of phytopathogens such as Maize Curvularia, potato wilt bacterium and Botryosphaeria berengeriana f. sp, only
Seldom mycelial growth.In addition, to blueberry branch-rot bacterium, fusarium graminearum, blueberry limb ulcer bacteria and tomato wilt
For bacterium, though there is mycelial growth, compared with the control, mycelial growth is weaker, illustrates that curcumin plays good inhibition.
In conclusion in the curcumin very potential prevention applied to phytopathogen.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to foregoing reality
Example is applied the present invention is described in detail, for those of ordinary skill in the art, still can be to foregoing implementation
Technical solution described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these are changed or replace
Change, the essence of appropriate technical solution is departed from the spirit and scope of claimed technical solution of the invention.
Claims (3)
1. curcumin is used to prevent and is existed by the purposes in the fungicide of the microbial plant disease of pathogenic, its feature in preparation
In：The phytopathogen is Deuteromycotina fungi and Ascomycotina fungi；
Effective concentration of curcumin is 15 mM -30mM in the fungicide；
The Deuteromycotina fungi includes blueberry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blueberry branch
Blight bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, cucumber rod spore leaf spot fungi, verticillium dahliae,
Potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens and Maize Curvularia；
The Ascomycotina fungi includes apple anthrax leaf spoting bacteria, blueberry limb ulcer bacteria, Botryosphaeria berengeriana f. sp, apple
Rotten pathogenic bacteria and pears rotten pathogenic bacteria.
2. curcumin according to claim 1 is being prepared for preventing the sterilization by the microbial plant disease of pathogenic
Purposes in agent, it is characterised in that：When the concentration of curcumin is 30mM in the fungicide, curcumin to verticillium dahliae,
Tobacco target spot bacterium, Botrytis cinerea, tobacco brown spot pathogen, apple anthrax leaf spoting bacteria, Valsa mali, soybean phytophthora
Pine root fungus, the inhibiting rate of 9 kinds of phytopathogens of tobacco black shank bacterium and tomato early blight bacterium are up to 99%.
3. curcumin according to claim 1 is being prepared for preventing the sterilization by the microbial plant disease of pathogenic
Purposes in agent, it is characterised in that：The plant is apple, peach, pears, tomato, blueberry, grape, tobacco, cucumber, cotton, water
Rice, corn, wheat and soybean.
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| CN109864075A (en) * | 2018-10-18 | 2019-06-11 | 贵州玉堂春农业发展有限责任公司 | A kind of tealeaves disease and pest control agent and preparation method thereof |
| CN112602535B (en) * | 2020-12-25 | 2023-07-04 | 广东润源中天生物科技有限公司 | Culture medium and culture method for ganoderma lucidum cultivation |
| CN115968884B (en) * | 2023-01-04 | 2024-05-24 | 山东青岛烟草有限公司 | Plant antifreeze agent containing curcumin and raffinose as well as preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101570496A (en) * | 2009-06-12 | 2009-11-04 | 乐山师范学院 | Curcumin-4-nitrogen-containing derivatives, and preparation method and uses thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101570496A (en) * | 2009-06-12 | 2009-11-04 | 乐山师范学院 | Curcumin-4-nitrogen-containing derivatives, and preparation method and uses thereof |
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| Title |
|---|
| A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin;Soheil Zorofchian Moghadamtousi等;《BioMed Research International》;20140429;第2014卷;第1-12页 * |
| Antifungal compounds from turmeric and nutmeg with activity against plant pathogens;Radwan, MM等;《FITOTERAPIA》;20141231;第99卷;第341-346页 * |
| Control of plant diseases using extrac ts from medicinal plants and fungi;J. R. Stangarlin等;《FORMATEX》;20111231;第1033-1042页 * |
| Fungicidal property of Curcuma longa L. rhizome-derived curcumin against phytopathogenic fungi in a greenhouse;Kim, MK等;《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》;20030312;第51卷(第6期);第1578-1581页 * |
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