CN105766906A - Use of curcumin in preparation of fungicide for preventing and controlling plant diseases caused by plant pathogens - Google Patents
Use of curcumin in preparation of fungicide for preventing and controlling plant diseases caused by plant pathogens Download PDFInfo
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Abstract
The invention provides use of curcumin in preparation of a fungicide for preventing and controlling plant diseases caused by plant pathogens. Indoor toxicity determination proves that curcumin has high inhibitory activity on plant pathogenic fungi. As a sterilizing agent, the curcumin has the advantages of high efficiency and low toxicity, and meets the requirement of chemical prevention and control of the plant diseases. The drug tolerance of the pathogens is enhanced due to use of a large quantity of sterilizing agents at present, and the conventional sterilizing agents have the defects of great environmental pollution and high residue content and direct threat food safety of human beings. Curcumin is a degradable, pollution-free and environment-friendly small molecular compound, has poor drug tolerance, and is safe to non-target organisms and human beings and live stocks, so that high quality of agricultural products, fruits and vegetables can be ensured, the requirement of sustainable development is met, and the research and market application prospects are wide.
Description
Technical field
The present invention relates to tool Fungicidal active compound, particularly to curcumin in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.
Background technology
Since 21 century, raising along with scientific and technological progress and social productive forces, the mankind create unprecedented wealth, meanwhile, the problems such as population increase, shortage of resources, environmental pollution, ecological disruption and frequent natural calamity become increasingly conspicuous, seriously hindering the sustainable development of agricultural, therefore agricultural production also undertakes unprecedented immense pressure.Estimating according to FAO (Food and Agriculture Organization of the United Nation), global grain and Cotton Production are lost in more than 10% because of disease throughout the year.Plant disease not only can cause the minimizing of crop yield, and to a certain extent also serious threat to the quality safety of agricultural product and international trade thereof.
The history of plant disease chemical prevention develops along with the generation of plant disease, and the practice of chemical prevention and application be the existing history in century more than one so far.The advantages such as chemical prevention has instant effect, fungicidal spectrum is wide, cost is low, easy to use, but long-term a large amount of uses of antibacterial can cause the environmental pollution such as soil, air, destroy ecological balance.Along with people's concern to food safety and problem of environmental pollution in recent years, some chemical bactericides have been strictly limited use at many developed countries and regions.People begin look for a kind of to the mankind with environmentally friendly and have the new control of plant disease strategy of good prevention effect.Novel green antibacterial is efficient and nontoxic, harmless, pollution-free, do not develop immunity to drugs, not only conform with people's demand to pollution-free food, and the sustainable development for agricultural provides guarantee, therefore, need more efficient, low toxicity, low-residual and environmentally friendly antibacterial is developed and application, play an important role in agricultural production.
Curcumin, has another name called Rhizoma Curcumae Longae；Chemical name: double; two (4-hydroxy 3-methoxybenzene the base)-1,6-heptadiene-3,5-diketone of (E, E)-1,7-；English name: curcumin, 6-Heptadiene-3,5-dione, 1,7-bis (4-hydroxy-3-methoxyphenyl)-, (E, E)-1, turmericyellow.No. CAS: 458-37-7, molecular formula: C21H20O6, molecular weight 368.38, molecular structural formula is:
Curcumin is orange-yellow crystalline powder.Fusing point 183 DEG C.Water insoluble, it is slightly soluble in ether and benzene, ethanol can be dissolved in during heating, be soluble in glacial acetic acid and aqueous slkali.Fast light, thermostability is poor.PH value is yellow when being 7.8, and pH value takes on a red color when being 9.2.Obtained by the root of Rhizoma Curcumae Longae.Plant origin has the tuber of zingiberaceous plant Radix Curcumae (CurcumaaromaticaSalisb), Rhizoma Curcumae Longae (C.longaL.) rhizome, Rhizoma Curcumae (C.zedoaria (Berg.) Rosc.) rhizome etc., it is also possible to chemosynthesis and biosynthesis.Reacted with acetylacetone,2,4-pentanedione by vanillin on producing and obtain.Main method: dissolve vanillin with anhydrous ethyl acetate, is subsequently adding butyl borate and the complex generated by acetylacetone,2,4-pentanedione, B2O3, then drips n-butylamine, drip off stirring 4-5h, place overnight.The 0.4N hydrochloric acid adding 60 DEG C next day continues stirring 1h, and with 50 DEG C of water bath heat preservations, makes to react completely.Divide the water layer of dereaction product, wash with water 3-4 time, leach curcumin, obtain crude product 2-3 time with ethyl acetate washing, obtain finished product with ethyl alcohol recrystallization.
Curcumin is a kind of natural dye, can directly make dyestuff and use, it is possible to as pH, color change interval 7.8～9.2, yellow redden brown.Additionally, curcumin has the effects such as function of gallbladder promoting, antibacterial, antioxidation, anti-nitrosation, carcinogenesis, antimutagenic, anti-liver poison, infection, antiviral and anticoagulation.It is alternatively arranged as food flavor, preservative, coloring agent, cosmetics, gastrotonica, analgesic, diuretic, choleretic, chemical industry is used for turmeric test paper processed, pH value 8～9, it is also possible in the qualitative analysis of boric acid.
At present, there is no both at home and abroad about curcumin the agricultural inhibiting report of pathogen.
Summary of the invention
It is an object of the invention to provide curcumin in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.The present invention passes through toxicity test, it was demonstrated that plant pathogenic fungi is had a good inhibitory activity by curcumin.Curcumin is as antibacterial, and it is efficient, low toxicity, is suitable for the requirement of plant disease chemical prevention.
For achieving the above object, the present invention is achieved by the following technical solutions:
The invention provides curcumin in preparation for preventing and treating the purposes in the antibacterial by the microbial plant disease of pathogenic.
Further: described phytopathogen is Deuteromycotina fungus, Ascomycotina fungus and Mastigomycotina fungus.
Further: in described antibacterial, effective working concentration of curcumin is 15mM-30mM.
Further: described Deuteromycotina fungus includes blue berry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blue berry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, Fructus Cucumidis sativi rod spore leaf spot fungi, verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium and Maize Curvularia.
Further: described Ascomycotina fungus includes Fructus Mali pumilae anthrax leaf spoting bacteria, blue berry branch ulcer bacteria, Botryosphaeria berengeriana f. sp, Valsa mali and pears rotten pathogenic bacteria.
Further: described Mastigomycotina fungus includes pathogen of soybean root rot and tobacco black shank bacterium.
Further: when in described antibacterial, the concentration of curcumin is 30mM, curcumin to the suppression ratio of the 9 kind of plant pathogen such as Botrytis cinerea, Valsa mali and tomato early blight bacterium up to 99%.
Further: described plant is Fructus Mali pumilae, Fructus Persicae, pears, Fructus Lycopersici esculenti, blue berry, Fructus Vitis viniferae, Nicotiana tabacum L., Fructus Cucumidis sativi, Cotton Gossypii, Oryza sativa L., Semen Maydis, Semen Tritici aestivi and Semen sojae atricolor.
Compared with prior art, advantages of the present invention and have the technical effect that the kind mechanism of action of current antibacterial is more single, the reproduction speed of pathogen is very fast, therefore resistance also produces comparatively fast, and pollutes greatly, and high residue threatens the food safety of the mankind.
The present invention passes through Toxicity Determination, it was demonstrated that plant pathogenic fungi is had a good inhibitory activity by curcumin.Curcumin, as antibacterial, has efficiently and the advantage of low toxicity, is suitable for the requirement of plant disease chemical prevention.The use of current bulk sterilization agent, causes that the Drug resistance of pathogen strengthens, and traditional antibacterial is big for environment pollution, residual height, directly threatens the food safety of the mankind.Along with cry pollution-free, pollution-free green food is improved by people day by day, green bactericide has become the important prevention and controls in agricultural production, and many green bactericide are come out one after another, and is widely used in producing, and achieves remarkable result.And curcumin is a kind of degradable, micromolecular compound pollution-free, environment amenable, and its Drug resistance is poor, to non-target organism and person poultry safety, ensure that the high-quality of agricultural product and fruit and vegerable, meet the requirement of sustainable development, its research and market application foreground are wide.
Accompanying drawing explanation
Fig. 1 is the curcumin bacteriostatic experiment result to Valsa mali in the present invention；
Fig. 2 is the curcumin bacteriostatic experiment result to tobacco brown spot pathogen in the present invention；
Fig. 3 is the curcumin bacteriostatic experiment result to Botrytis cinerea in the present invention；
Fig. 4 is the curcumin bacteriostatic experiment result to tomato early blight bacterium in the present invention；
Fig. 5 is the curcumin bacteriostatic experiment result to fusarium graminearum in the present invention；
Fig. 6 is the curcumin bacteriostatic experiment result to pathogen of soybean root rot in the present invention；
Fig. 7 is the curcumin bacteriostatic experiment result to tomato wilt bacterium in the present invention；
Fig. 8 is the curcumin bacteriostatic experiment result to pears rotten pathogenic bacteria in the present invention；
Fig. 9 is the curcumin bacteriostatic experiment result to Tobacco target spot bacterium in the present invention；
Figure 10 is the bacteriostatic experiment result that in the present invention, blue berry is intended stem point branch-rot bacterium by curcumin；
Figure 11 is the curcumin bacteriostatic experiment result to tobacco black shank bacterium in the present invention；
Figure 12 is the curcumin bacteriostatic experiment result to blue berry branch-rot bacterium in the present invention；
Figure 13 is the curcumin bacteriostatic experiment result to potato wilt bacterium in the present invention；
Figure 14 is the curcumin bacteriostatic experiment result to Botryosphaeria berengeriana f. sp in the present invention；
Figure 15 is the curcumin bacteriostatic experiment result to rice blast fungus in the present invention；
Figure 16 is the curcumin bacteriostatic experiment result to Ustilaginoidea virens in the present invention；
Figure 17 is the curcumin bacteriostatic experiment result to blue berry branch ulcer bacteria in the present invention；
Figure 18 is the curcumin bacteriostatic experiment result to Fructus Cucumidis sativi rod spore leaf spot fungi in the present invention；
Figure 19 is the curcumin bacteriostatic experiment result to verticillium dahliae in the present invention；
Figure 20 is the curcumin bacteriostatic experiment result to Maize Curvularia in the present invention；
Figure 21 is the curcumin bacteriostatic experiment result to Fructus Mali pumilae anthrax leaf spoting bacteria in the present invention；
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, technical scheme is described in further detail.
Embodiment 1
One, experiment material
Curcumin is the finished product bought from Sigma company.Weighing 7.37g curcumin in the dimethyl sulfoxide of 20ml, be made into the mother solution of 1M, filtration sterilization 4 DEG C preservation is stand-by.
Phytopathogen used in this experiment is the strain that laboratory 4 DEG C preserves, and the culture medium of employing is potato culture (being called for short PDA).
PDA culture medium prescription: Rhizoma Solani tuber osi (peeling) 200g, glucose 20g, agar 15g, tap water 1000ml, natural PH
Compound method: Rhizoma Solani tuber osi is cleaned peeling, 200g is claimed to be cut into small pieces, add water well-done (boiling 20-30 minute, can be poked by Glass rod), by eight layers of filtered through gauze in beaker, need to add 15-20g agar according to experiment, adding 20g glucose, stir, after fully dissolving, slightly cooling supplies water to 1000ml, after subpackage, 121 DEG C of sterilizings 20 minutes, standby after cooling.
Two, experimental technique
Adopt growth rate method.
1, first by stand-by for 21 kind of plant pathogen, 25 DEG C of cultivation about 2d on PDA plate.
2, the heating of PDA culture medium being dissolved, be cooled to 45-50 DEG C, the curcumin being separately added into variable concentrations makes the culture medium (adding the dimethyl sulfoxide of respective volume in comparison) containing 0,7.5,15 and 30mM medicinal liquid, and pours cooling in culture dish respectively into.
3, with sterile working's formality, beat at each bacterial strain mycelia edge (upgrowth situation consistent) cultivating 2d with card punch and take circle bacterium cake (diameter 0.60cm) as far as possible, choose to pastille flat board central authorities with Inoculating needle again, then culture dish is inverted in incubator (25 DEG C) and cultivates.
4, the growing state of different time Observe and measure mycelia after processing, and adopt decussation method record diameter and process data, calculate suppression ratio and take pictures.
Suppression ratio (%)=(comparison hyphal diameter-process hyphal diameter)/comparison hyphal diameter × 100
Each process is repeated 3 times.
Three, the curcumin fungistatic effect to 21 kind of plant pathogen mycelial growths
1. verticillium dahliae: the pathogen causing cotton verticillium wilt is the verticillium dahliae that Verticillium belongs to.The host range of this pathogen is extremely wide, abroad reports 38 section 660 kind of plant, wherein crops 184 kinds of can causing harm, 153 kinds of weeds.China is identified, and host plant at least 20 section 80 kinds, field crop includes Cotton Gossypii, Helianthi, Fructus Solani melongenae, spicy, Fructus Lycopersici esculenti, Nicotiana tabacum L., Rhizoma Solani tuber osi, Fructus Melo, Citrullus vulgaris, Fructus Cucumidis sativi, Semen arachidis hypogaeae, Kidney bean, Semen phaseoli radiati, Semen sojae atricolor, Semen Sesami, Radix Betae etc..This pathogenic bacteria can make host plant produce verticillium wilt, worldwide causes great economic loss.Verticillium wilt preventing and treating difficulty, existing means of prevention such as Biological control, chemical prevention, conventional genetic breeding etc. are all unable to reach desirable prevention effect.
2. tobacco black shank bacterium: this pathogenic bacteria can endanger all cultivations Nicotiana tabacum L. such as flue-cured tobacco, air-curing of tobacco leaves, suncured tabacco, Turkish tobaccos, burley tobaccos, causes black shank.Reaching 22 DEG C at temperature on average, rainy time and low-lying humid area, black shank spreads quickly, whole vega can be made to destroy in 1～2 week, destructive extremely strong, often results in the whole strain death of cigarette strain after field infestation.Black shank is a kind of destructive soil-borne disease, is also one of global Main Tobacco Diseases.Once occur in production, if processed not in time, will result in serious economic loss.The host range of this pathogenic bacteria is narrower, and Nicotiana tabacum L. is its unique host under field conditions (factors).Although host range is larger under artificial inoculation conditions, but also only infect a few plant such as stem and Rhizoma Solani tuber osi, Fructus Solani melongenae, Fructus Capsici, Semen Ricini, Fructus Mali pumilae, striped calabash of tomato seedling and fruit, the cotton boll of Cotton Gossypii, papaw.
3. pathogen of soybean root rot: soybean phytophthora root rot is a kind of by the microbial disease of soybean phytophthora, although its origin is relatively later, but it is the phytophthora blight that in field crop, unique harm is serious.Its hazard area is big, degree weight, and oneself is by the extremely strong survival ability of one of row Semen sojae atricolor destructive disease, the characteristic passed due to its soil and pathogen thereof, this disease so far at world's major soybean production areas still in the gesture expanded.This pathogenic bacteria host range is narrow, only infects Semen sojae atricolor.Semen sojae atricolor can be infected at all period of duration of Semen sojae atricolor.Generally issue seriously ill in moist rainy weather condition, disease short incubation period, then infect often, and propagate and spread rapidly, host is dead in flakes, and little individual plant is fallen ill.
4. tomato early blight bacterium: this disease, except directly endangering stem, leaf, fruit, may also suppress tomato growth and fruit is formed, and open country and protecting field are injured all heavier, long-term underproduction 20%-30%, up to more than 50% time serious, even have no harvest.This cause of disease host range is extensive, except harm Fructus Lycopersici esculenti, also can endanger the solanaceous vegetable crops such as Fructus Solani melongenae, Fructus Capsici and Rhizoma Solani tuber osi.
5. rice blast fungus: rice blast is by the microbial a kind of disease of rice blast, is one of important in the world rice disease, and same banded sclerotial blight, bacterial leaf-blight are listed in the big disease of Oryza sativa L. three.Typically result in the Oryza sativa L. underproduction 10% 20%, time serious can the underproduction 40% 50%, even have no harvest.This pathogenic bacteria not only infects Oryza sativa L., moreover it is possible to infects the crops such as Semen Tritici aestivi, Fructus Hordei Vulgaris and foxtail millet, even infects the root of plant, agricultural production causes serious harm.
6. fusarium graminearum: head blight is a kind of destructive disease, can cause fringe rotten, causes Severe Reduction and quality to reduce.Along with the change of global warming, cropping system and mode, wheat scab constantly spreads extension, often results in wheat yield, quality reduction, and containing mycotoxin in the wheat seed infected, can cause person poultry poisoning and serious disease.This pathogenic bacteria is except harm Semen Tritici aestivi, moreover it is possible to infect the grassy weed such as the multiple gramineous crop such as Fructus Hordei Vulgaris, Herba bromi japonici, Oryza sativa L., Semen Maydis and goose hat grass, additionally, also can infect the crops such as Semen sojae atricolor, Cotton Gossypii, Radix Ipomoeae.
7. tomato wilt bacterium: the specialization of tomato wilt bacterium is extremely strong, only endangers Fructus Lycopersici esculenti.This bacterium mainly infects flora conduit tissue, causes plant wilt by blocking xylem material and Water Transportation.It is the soil-borne disease of a kind of serious harm quality of vegetable and yield, and the Seedling Stage of Fructus Lycopersici esculenti is all subject to infecting of wilt during Adult plant.Tomato wilt is again wilt disease, occurs serious in Lao Caipeng room.
8. Valsa mali: apple tree canker is the trunk disease that apple tree is main, the most serious.This disease can also infect fruit except infecting branch, causes deadwood, extremely sets, and causes the garden of ruining time serious, thus causing serious yield and economic loss.Apple rot pathogen has and compares host plant widely, and harm includes the common fruit tree of Fructus Mali pumilae, Lou Tao, Fructus Persicae and pears etc..
9. potato wilt bacterium: Fusarium oxysporum is the Main Pathogenic Bacteria of potato wilt, and this bacterium is a kind of universal soil-borne disease fungal pathogens, host range is very extensive, can cause the generation of the various plants droops such as Solanaceae, melon, Fructus Musae, pulse family, cotton and flowers.After host is infected by Fusarium oxysporum, Symptoms is varied, generally results in vascular bundle brown stain, and plant is wilted withered, bulb and root rot, and plant strain growth is weak.Potato wilt is a kind of important fungoid soil-borne disease in recent years Rhizoma Solani tuber osi worked the mischief, in the existing generation in a lot of areas, especially even more serious at Rhizoma Solani tuber osi weight weak ground occurrence degree, directly affects potato yield and economic benefit thereof.
10. Fructus Mali pumilae anthrax leaf spoting bacteria: Fructus Mali pumilae anthrax leaf blight is by the microbial a kind of leaf diseases of anthrax, the kinds such as the loud, high-pitched sound of main harm Fructus Mali pumilae is drawn, Jin Guan, Qiao Najin, Qin Guan, causing apple tree to fall leaves in a large number and fruit seriously rots, tree vigo(u)r seriously undermines, and causes that next year has no harvest.Fuji, red magnitude kind height are disease-resistant, and the loud, high-pitched sound of morbidity is drawn and contrasted clearly in orchard with the adjacent Fuji apple trees do not fallen ill.Fructus Mali pumilae anthrax leaf blight main harm blade, and cause a large amount of fallen leaves.
11. blue berry branch-rot bacterium: this pathogenic bacteria belongs to plan Pestalotia, be a kind of important phytopathogen, can the plant of parasitic about section more than 50, show as tikka after hazard of plant, rot, the symptom such as ulcer, have a strong impact on the yield and quality of product.Therefore, the production of farm-forestry crop is had important economic implications by research plan pestalotia bacteria.At present, the blue berry main breed in production, Ru Lanfeng, duke and blue beauty etc., all there is branch-rot to occur, its fruit yield of appreciable impact and quality.
12. tobacco brown spot pathogen: Alternaria alternate is the fungal disease of harm China tobacco leaf production, can not only make quality of tobacco decline and blade incompleteness is damaged, moreover it is possible to reducing its industrial application and be worth, susceptible Nicotiana tabacum L. makes the mouthfeel of Nicotiana tabacum L. decline owing to tobacco leaf ingredient differs.Alternaria altemata host range is relatively wide, outside smoke abatement grass, also can infect the various plants such as Cotton Gossypii, Semen arachidis hypogaeae, Semen sojae atricolor, Fructus Lycopersici esculenti, Fructus Persicae, Lee, Semen Tritici aestivi, cause the symptom such as speckle, root rot.
13. Ustilaginoidea virens: rice green smut, also known as pseudo-smut, green smut, paddy blossom disease, Calomelas disease, is commonly called as " high yield fruit ", being infected, by the green pyrenomycetes of rice or rice Clavicipitaceae, a kind of rice fungus diseases evil that Rice Panicle occurs, in the world, all there is generation in main rice district.This disease not only causes production loss, and has a strong impact on rice quality, and oneself becomes one of important disease of affecting China's Rice Production and rice quality.
14. Fructus Cucumidis sativi rod spore leaf spot fungi: this pathogenic bacteria host range is extensive, and circulation way is various.Further investigation reveals that in recent years, the leaf spot of the vegetables such as the Fructus Cucumidis sativi rod microbial Fructus Cucumidis sativi of spore leaf spot, Fructus Lycopersici esculenti occur in 11 province, city and region's large area such as the Shandong of China, Hebei, Liaoning, the Inner Mongol, cause serious economic loss.
15. blue berry intends stem point branch-rot bacterium: it is a kind of important trunk disease on blue berry that blue berry intends stem point branch-rot.Host range is relatively wide, mainly has the plants such as Larch, Fructus Persicae, pear, Vaccinium, Jianshi maple.
16. Botryosphaeria berengeriana f. sp: it is sick that ring rot of apple has another name called scurf, wheel stricture of vagina decayed fruit, is the important biomolecule disaster on Fructus Mali pumilae branch portion and fruit, often cause that Fructus Mali pumilae branch bark is coarse, locality is downright bad and fruit rot.Ill plant fruit-setting rate is low, causes tree asthenia weak and yield reduces, and even garden is ruined in total crop failure.In recent years, along with the commerial growing of susceptible kind Fuji apple, ring rot of apple sickness rate increases year by year, and hazard area constantly expands, it has also become the serious plant disease in Chinese apple production, the sustainable development of Apple Industry in serious threat.
17. pears rotten pathogenic bacteria: pears canker has another name called bark rot, the major branch of main harm pear tree and side shoot, causing that pear tree gesture is weak, fruit yield and quality decline.This disease has that generation area is wide, sickness rate is high, unmanageable feature.All there is generation in China's pears main producing region, especially occur with Xinjiang, northwest, North China, northeast and other places serious.Falling ill serious theatre, body disease scar is innumerable, branch is incomplete for tree, causes a large amount of Si Shuhuohui garden.
18. blue berry branch ulcer bacteria: the pathogen Botryosphaeria causing blue berry branch Peptic Ulcers is a universal fungus of class, the Tree resistance caused is the great disease occurred in world wide.The host of its harm is very wide, reach 45 genus (broad leaf tree 40 genus, 5, coniferous tree genus), the harm of woods fruit tree is serious, the symptoms such as the branch ulcer withered death of the host plants such as Eucalyptus, willow, Fructus Mali pumilae, Punica granatum L., peach tree, Semen Cassiae, Fructus Myricae rubrae, Semen Castaneae, dry rot, gummosis can be caused.
19. Botrytis cinerea: grape grey mould is the Common Diseases that the microbial Fructus Vitis viniferae of Botrytis cinerea produces, also it is endanger one of maximum disease, occurs general.This disease is particularly acute in south China area and north protecting field Fructus Vitis viniferae produce, and oneself becomes the Major Diseases of restriction northern China facility culture grapes industry development.Destructive disease in this disease or storage, affects very big on yield and quality, and the Fructus Vitis viniferae post-harvest loss caused because of gray mold every year, about 20% 30%, causes huge economic loss.This pathogenic bacteria is the facultative parasite that a kind of host range is very wide, can infect various fruits, vegetable and flowers.
20. Maize Curvularia: Maize Curvularia Leaf Spot Disease, also known as plan eye spot, black points, maize leaf of mainly causing harm, also endangers sheath and bract sometimes, and its symptom variation is very big in different cultivars.This pathogenic bacteria host range is wider, it is possible to parasitize on the fruit of Fructus Lycopersici esculenti, Fructus Capsici, it is also possible to colonize on Oryza sativa L., Sorghum vulgare Pers., Semen Tritici aestivi and some lawns so that these plant generation diseases.Maize Curvularia Leaf Spot Disease is a kind of disease that China North China, northeast corn producing region generally occur in recent years, owing to this disease is sudden disease, spread rapidly, time serious, leaf portion scab is intensive in flakes, severe disease plot diseased plant rate and sick leaf rate are up to 100%, it has also become one of the important disease in China's Semen Maydis producing region.
21. Tobacco target spot bacterium: Tobacco target spot is by the microbial tikka class disease of miliary damping-off, this pathogenic bacteria main harm blade forms scab, and scab necrosis part is frangible forms perforation, and when occurring serious, scab is in flakes, and plant disease epidemic speed, therefore constitutes serious threat to tobacco leaf production.The host range of this pathogenic bacteria is extensive, is possible not only to infect Nicotiana tabacum L., can infect 200 various plants in natural situation, cereal crops and Radix Betae, Fructus Solani melongenae, Fructus Lycopersici esculenti, Herba Solani Nigri, honor fiber crops and the petunia etc. such as including Semen Maydis, Sorghum vulgare Pers., Cotton Gossypii, wheat and barley, Oryza sativa L., Semen sojae atricolor.Additionally, this pathogenic bacteria can infect 188 kind of plant of 32 sections under artificial inoculation conditions.
The in vitro bacteriostasis life of 21 kind of plant pathogen is surveyed result in Table 1 by curcumin.
Table 1 curcumin inhibitory action measurement result (6d) to 21 kinds of pathogen mycelial growths
Note: in test, each process sets three repetitions, in table, data are the meansigma methods repeated for three times.
As can be known from Table 1, along with the rising of curcumin concentration, the inhibition of 21 kind of plant pathogen is also improved by successively.When curcumin concentration is 30mM, 21 kind of plant pathogen of Deuteromycotina, Ascomycotina and Mastigomycotina are all had good inhibition by it, suppression ratio is all more than 85%, wherein to the suppression ratio of the 17 kind of plant pathogen such as Maize Curvularia, potato wilt bacterium and Botryosphaeria berengeriana f. sp more than 90%.Especially the fungistatic effect of 9 kinds of pathogen such as Botrytis cinerea, Valsa mali and soybean scab bacterium is best, it, all more than 99%, can effectively be prevented and treated by its suppression ratio.
Curcumin is to the fungistatic effect picture of 21 kind of plant pathogen mycelial growths such as shown in Fig. 1-2 1, and when the concentration of curcumin is when 30mM, 9 kinds of pathogen such as Botrytis cinerea, Valsa mali and soybean scab bacterium are almost without mycelial growth.And for the 8 kind of plant pathogen such as Maize Curvularia, potato wilt bacterium and Botryosphaeria berengeriana f. sp, only little mycelial growth.Additionally, for blue berry branch-rot bacterium, fusarium graminearum, blue berry branch ulcer bacteria and tomato wilt bacterium, though there being mycelial growth, but compared with the control, mycelial growth is more weak, illustrate that curcumin serves good inhibition.In sum, in the very potential preventing and treating being applied to phytopathogen of curcumin.
Above example is only in order to illustrate technical scheme, but not is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent replacement；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of present invention technical scheme required for protection.
Claims (8)
1. curcumin is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic.
2. curcumin according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described phytopathogen is Deuteromycotina fungus, Ascomycotina fungus and Mastigomycotina fungus.
3. curcumin according to claim 1 and 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: in described antibacterial, effective working concentration of curcumin is 15mM-30mM.
4. curcumin according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Deuteromycotina fungus includes blue berry and intends stem point branch-rot bacterium, tomato wilt bacterium, tomato early blight bacterium, blue berry branch-rot bacterium, tobacco brown spot pathogen, rice blast fungus, fusarium graminearum, Fructus Cucumidis sativi rod spore leaf spot fungi, verticillium dahliae, potato wilt bacterium, Botrytis cinerea, Ustilaginoidea virens, Tobacco target spot bacterium and Maize Curvularia.
5. curcumin according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Ascomycotina fungus includes Fructus Mali pumilae anthrax leaf spoting bacteria, blue berry branch ulcer bacteria, Botryosphaeria berengeriana f. sp, Valsa mali and pears rotten pathogenic bacteria.
6. curcumin according to claim 2 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described Mastigomycotina fungus includes pathogen of soybean root rot and tobacco black shank bacterium.
7. curcumin according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterized in that: when in described antibacterial, the concentration of curcumin is 30mM, curcumin to the suppression ratio of the 9 kind of plant pathogen such as Botrytis cinerea, Valsa mali and tomato early blight bacterium up to 99%.
8. curcumin according to claim 1 is preparing the purposes for preventing and treating in the antibacterial by the microbial plant disease of pathogenic, it is characterised in that: described plant is Fructus Mali pumilae, Fructus Persicae, pears, Fructus Lycopersici esculenti, blue berry, Fructus Vitis viniferae, Nicotiana tabacum L., Fructus Cucumidis sativi, Cotton Gossypii, Oryza sativa L., Semen Maydis, Semen Tritici aestivi and Semen sojae atricolor.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109864075A (en) * | 2018-10-18 | 2019-06-11 | 贵州玉堂春农业发展有限责任公司 | A kind of tealeaves disease and pest control agent and preparation method thereof |
| CN112602535A (en) * | 2020-12-25 | 2021-04-06 | 广东润源中天生物科技有限公司 | Ganoderma lucidum culture medium and culture method |
| CN115968884A (en) * | 2023-01-04 | 2023-04-18 | 青岛农业大学 | Plant antifreeze agent containing curcumin and raffinose and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570496A (en) * | 2009-06-12 | 2009-11-04 | 乐山师范学院 | Curcumin-4-nitrogen-containing derivatives, and preparation method and uses thereof |
-
2016
- 2016-03-30 CN CN201610191056.9A patent/CN105766906B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101570496A (en) * | 2009-06-12 | 2009-11-04 | 乐山师范学院 | Curcumin-4-nitrogen-containing derivatives, and preparation method and uses thereof |
Non-Patent Citations (4)
| Title |
|---|
| J. R. STANGARLIN等: "Control of plant diseases using extrac ts from medicinal plants and fungi", 《FORMATEX》 * |
| KIM, MK等: "Fungicidal property of Curcuma longa L. rhizome-derived curcumin against phytopathogenic fungi in a greenhouse", 《JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY》 * |
| RADWAN, MM等: "Antifungal compounds from turmeric and nutmeg with activity against plant pathogens", 《FITOTERAPIA》 * |
| SOHEIL ZOROFCHIAN MOGHADAMTOUSI等: "A Review on Antibacterial, Antiviral, and Antifungal Activity of Curcumin", 《BIOMED RESEARCH INTERNATIONAL》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109864075A (en) * | 2018-10-18 | 2019-06-11 | 贵州玉堂春农业发展有限责任公司 | A kind of tealeaves disease and pest control agent and preparation method thereof |
| CN112602535A (en) * | 2020-12-25 | 2021-04-06 | 广东润源中天生物科技有限公司 | Ganoderma lucidum culture medium and culture method |
| CN115968884A (en) * | 2023-01-04 | 2023-04-18 | 青岛农业大学 | Plant antifreeze agent containing curcumin and raffinose and preparation method and application thereof |
| CN115968884B (en) * | 2023-01-04 | 2024-05-24 | 山东青岛烟草有限公司 | Plant antifreeze agent containing curcumin and raffinose as well as preparation method and application thereof |
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